JPH0984528A - Feed for pisciculture - Google Patents
Feed for piscicultureInfo
- Publication number
- JPH0984528A JPH0984528A JP7242853A JP24285395A JPH0984528A JP H0984528 A JPH0984528 A JP H0984528A JP 7242853 A JP7242853 A JP 7242853A JP 24285395 A JP24285395 A JP 24285395A JP H0984528 A JPH0984528 A JP H0984528A
- Authority
- JP
- Japan
- Prior art keywords
- feed
- fish
- oligopeptide
- protein
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、養殖魚の連鎖球菌
症等、その病原菌進入部位が主として消化管である細菌
性疾病に対する抗病性を向上させ、かつ低酸素水塊等の
環境悪化に対する抵抗性をも向上させて、これら養殖魚
の生残率の向上および成育を促進させるための飼料に関
する。TECHNICAL FIELD [0001] The present invention improves the resistance to streptococcal disease of farmed fish and other bacterial diseases in which the pathogen entry site is mainly the digestive tract and resistance to environmental deterioration such as hypoxic water mass. The present invention also relates to a feed for improving the survival rate and improving the survival rate and growth of these cultured fish.
【０００２】[0002]
【従来の技術】養殖漁業の発展に伴い、全国各地の内湾
等養殖適地ではどこも養殖場が団地化し過密状態で養殖
が行われている。その結果、各種疾病の病原菌は常在状
態となり、病害の発生は慢性化している。この対策とし
て各種の抗生物質、合成抗菌剤等が使用されているが、
これら薬剤に対する耐性菌の出現により完全な治療、防
除が出来ず毎年多大な被害を被っている。2. Description of the Related Art With the development of aquaculture, the aquaculture farms are integrated into aquaculture areas in all suitable places for aquaculture such as inland bays throughout the country. As a result, pathogenic bacteria of various diseases become resident, and the disease has become chronic. Various antibiotics, synthetic antibacterial agents, etc. are used as measures against this,
Due to the emergence of resistant bacteria to these drugs, complete treatment and control cannot be performed, and a great deal of damage is incurred each year.
【０００３】また、近年の沿岸海域の富栄養化および養
殖場の自家汚染等漁場環境悪化による漁業被害も大きな
問題である。漁場環境悪化が原因になって起こるとされ
る海洋現象の一つに低酸素水塊の出現がある。この現象
は、初秋気温下降期に多く発生し、海底部から湧昇して
きた低酸素水および硫化物等の有害物に養殖魚がさらさ
れて被害を受ける。しかし、現在のところこの低酸素水
塊による漁業被害を防止する有効な手段はまだ開発され
ていない。Further, damage to the fishing industry due to deterioration of the fishing ground environment such as eutrophication of coastal sea areas and self-pollution of farms in recent years is also a serious problem. The appearance of low oxygen water mass is one of the ocean phenomena that is thought to occur due to the deterioration of the fishing ground environment. This phenomenon frequently occurs during the early fall of the temperature and the cultured fish are exposed to and harmed by the low oxygen water and sulfides and other harmful substances that have risen from the sea floor. However, at present, no effective means has been developed to prevent the fishery damage caused by this low oxygen water mass.
【０００４】近年、魚類疾病対策としてこれら抗菌抗生
物質等による対症療法だけでなく、ワクチンや免疫賦活
剤による病害予防の研究も盛んに行われているが、その
実用化はまだ緒についたばかりと言えよう。たとえば、
魚類ワクチンではビブリオ病ワクチン等まだ一部の魚
種、一部の疾病について実用化されているに過ぎず、免
疫賦活剤においても菌体膜成分、または菌体膜成分から
抽出したグルカンをはじめとする多糖類が一部利用され
ているに過ぎない〔魚病研究 29, 1, 11 (1994)、魚病
研究 29, 2, 73 (1994)〕。In recent years, as measures against fish diseases, not only symptomatic treatments with these antibacterial antibiotics but also disease prevention with vaccines and immunostimulants have been actively conducted, but it can be said that its practical application has just begun. See. For example,
In fish vaccines, only some fish species such as Vibrio disease vaccines and some diseases have already been put to practical use, and in the immunostimulant, bacterial cell components or glucans extracted from bacterial cell components have been used. The polysaccharides used are only partially utilized [Fish Disease Research 29 , 1, 11 (1994), Fish Disease Research 29 , 2, 73 (1994)].
【０００５】一方、食品工業の分野では近年、コーンペ
プチド（トウモロコシ蛋白加水分解ペプチド）、小麦ペ
プチド（小麦蛋白加水分解ペプチド）等植物蛋白由来ペ
プチドの機能性が注目されており〔食品と開発 27, 46
(1992)〕、養魚における栄養補給および病気発生の抑
制を目的とし、大豆ペプチドの利用開発が進められてい
る〔月刊フードケミカル食品と開発 8, 64 (1988)〕。
しかしながら、コーンペプチド（トウモロコシ蛋白加水
分解ペプチド）、小麦ペプチドを魚類の細菌性疾病予防
に用いた報告、および魚類の抵抗性増強に利用した報告
はない。On the other hand, in the field of food industry, the functionality of plant protein-derived peptides such as corn peptide (corn protein hydrolyzing peptide) and wheat peptide (wheat protein hydrolyzing peptide) has been attracting attention in recent years [food and development 27 , 46
(1992)], the use development of soybean peptides is being promoted for the purpose of nutritional supplementation and suppression of disease occurrence in fish farming [Monthly Food Chemical Foods and Development 8 , 64 (1988)].
However, there are no reports of using corn peptide (maize protein hydrolyzing peptide) or wheat peptide for the prevention of bacterial diseases in fish, and no report of using it for enhancing the resistance of fish.
【０００６】[0006]
【発明が解決しようとする課題】本発明の目的は、養殖
ブリにおいて慢性的な発生が見られる連鎖球菌症に対す
る抗病性の向上、および突発的に発生する低酸素水塊等
養殖場の環境悪化による養殖魚の斃死率の低下をはかり
漁業被害を低減するための飼料を提供することにある。DISCLOSURE OF THE INVENTION The object of the present invention is to improve the anti-disease properties against streptococcal disease, which is chronically observed in cultured yellowtail, and the environment of aquaculture farms such as hypoxic water lumps that suddenly occur. It is intended to provide feed for reducing the mortality rate of farmed fish due to deterioration and reducing damage to fisheries.
【０００７】[0007]
【課題を解決するための手段】本発明者らは、上記課題
を解決すべく、鋭意研究を重ねた結果、小麦蛋白加水分
解ペプチド、トウモロコシ蛋白加水分解ペプチド等の植
物蛋白由来ペプチドを添加した飼料を養殖魚に投与する
ことにより、ブリ連鎖球菌症等の細菌性疾病に対する抗
病性を向上させ、また、低酸素水塊等の養殖場環境悪化
に対する抵抗性を増強してこれら養殖魚の斃死率を低下
させることを見出し、本発明を完成するに至った。本発
明は、小麦蛋白加水分解ペプチドおよびトウモロコシ蛋
白加水分解ペプチド等の植物蛋白由来ペプチドを含有し
てなる養魚用飼料に関する。[Means for Solving the Problems] As a result of intensive studies to solve the above problems, the present inventors have found that a feed containing a peptide derived from a plant protein such as wheat protein hydrolyzed peptide and corn protein hydrolyzed peptide has been added. To improve the disease resistance against bacterial diseases such as Streptococcus pneumoniae, and to enhance resistance to deterioration of the farm environment such as hypoxic water mass, thereby increasing the mortality rate of these cultured fish. It was found that the above-mentioned decrease was achieved, and the present invention was completed. TECHNICAL FIELD The present invention relates to a fish feed containing a peptide derived from a plant protein such as a wheat protein hydrolyzed peptide and a corn protein hydrolyzed peptide.
【０００８】[0008]
【発明の実施の形態】本発明の養殖魚として、ブリ、カ
ンパチ、マダイ、ヒラメ、トラフグ等をあげることがで
きる。本発明に用いる小麦またはトウモロコシ蛋白質由
来加水分解ペプチドとしては、小麦またはトウモロコシ
起源の蛋白質を、酸、アルカリ、加熱または種々のプロ
テアーゼ等の酵素処理により加水分解して得られる、ペ
プチド鎖長２〜２０、中でも２〜６個のアミノ酸からな
るオリゴペプチドを主成分としてなるペプチドが好適で
ある。BEST MODE FOR CARRYING OUT THE INVENTION Examples of the cultured fish of the present invention include yellowtail, amberjack, red sea bream, flounder, and tiger puffer fish. The wheat or corn protein-derived hydrolyzed peptide used in the present invention is a peptide having a peptide chain length of 2 to 20, which is obtained by hydrolyzing a protein of wheat or corn origin by acid, alkali, heat, or enzymatic treatment with various proteases. Among them, a peptide containing an oligopeptide consisting of 2 to 6 amino acids as a main component is preferable.
【０００９】該ペプチドは、特開平２−２９５４３７、
特開平２−４９５７９、特開平５−２３６９０９、特開
平６−２４５７９０等に記載の方法に準じて取得するこ
とができる。即ち、酸性、中性、アルカリ性プロテアー
ゼ処理等を組み合わせて取得することができる。酸性プ
ロテアーゼとしては、微生物由来のプロテアーゼであ
る、ラピターゼ（武田化学社製）、モルシン（シグマ社
製）、動物由来のプロテアーゼである、トリプシン、ペ
プシン等をあげることができる。The peptide is described in JP-A-2-295437,
It can be obtained according to the method described in JP-A-2-49579, JP-A-5-236909, JP-A-6-245790 and the like. That is, it can be obtained by combining acidic, neutral, and alkaline protease treatments. Examples of the acidic protease include microbial proteases such as rapitase (manufactured by Takeda Chemical Co., Ltd.), morcin (manufactured by Sigma), and animal-derived proteases such as trypsin and pepsin.
【００１０】酸性プロテアーゼは基質蛋白質１００ｇ当
たり０．０５〜２ｇ（１万〜８０万ユニット）、好まし
くは０．１〜１ｇ（２万〜４０万ユニット）の割合で使
用するのがよい。酸性プロテアーゼによる加水分解処理
は、通常、ｐＨ３〜４、２０〜６０℃、５〜２４時間行
う。The acid protease is used in a proportion of 0.05 to 2 g (10,000 to 800,000 units), preferably 0.1 to 1 g (20,000 to 400,000 units) per 100 g of the substrate protein. The hydrolysis treatment with acidic protease is usually performed at pH 3 to 4, 20 to 60 ° C. for 5 to 24 hours.
【００１１】アルカリ性プロテアーゼとしては、微生物
由来のプロテアーゼである、アルカラーゼ０．６Ｌ、エ
スペラーゼ８．０Ｌ、サビナーゼ８．０Ｌ（いずれもノ
ボインダストリージャパン社製）、オリエンタラーゼ２
２（上田化学工業社製）、プロチンＡ（大和化成社
製）、好アルカリ性細菌（Bacillus. No.221）由来のア
ルカリ性プロテアーゼ（名糖産業社製）等をあげること
ができる。The alkaline proteases are microbial-derived proteases such as Alcalase 0.6L, Esperase 8.0L, Savinase 8.0L (all manufactured by Novo Industry Japan), Orientalase 2
2 (manufactured by Ueda Chemical Industry Co., Ltd.), Protin A (manufactured by Daiwa Kasei Co., Ltd.), alkaline protease (manufactured by Meito Sangyo Co., Ltd.) derived from an alkalophilic bacterium ( Bacillus No. 221), and the like.
【００１２】アルカリ性プロテアーゼは基質蛋白質１０
０ｇ当たり０．０５〜２ｇ（１万〜４０万ユニット）、
好ましくは０．１〜１ｇ（２万〜２０万ユニット）の割
合で使用するのがよい。アルカリ性プロテアーゼによる
加水分解処理は、通常、ｐＨ８〜１２、好ましくはｐＨ
９〜１１、４０〜７０℃、好ましくは５０〜６０℃、５
〜３０時間行う。Alkaline protease is a substrate protein 10
0.05 to 2 g per 0 g (10,000 to 400,000 units),
It is preferable to use it at a rate of 0.1 to 1 g (20,000 to 200,000 units). The hydrolysis treatment with alkaline protease is usually pH 8 to 12, preferably pH.
9-11, 40-70 ° C, preferably 50-60 ° C, 5
Do for ~ 30 hours.
【００１３】中性プロテアーゼとしては、微生物または
植物由来の中性プロテアーゼを用いることができ、プロ
テアーゼアマノＮ（天野製薬社製）、アロアーゼＡＰ−
１０（ヤクルト社製）、オリエンターゼ９０Ｎ（上田化
学工業社製）、プロチンＰＣ１０Ｆ（大和化成社製）等
をあげることができる。中性プロテアーゼは基質蛋白質
１００ｇ当たり０．０５〜２ｇ（２万〜８０万ユニッ
ト）、好ましくは０．１〜１ｇ（１万〜４０万ユニッ
ト）の割合で使用するのがよい。As the neutral protease, a microbial or plant-derived neutral protease can be used. Protease Amano N (manufactured by Amano Pharmaceutical Co., Ltd.), Aroase AP-
10 (manufactured by Yakult), Orientase 90N (manufactured by Ueda Chemical Industry Co., Ltd.), Protin PC10F (manufactured by Daiwa Kasei Co., Ltd.) and the like. The neutral protease is used in a ratio of 0.05 to 2 g (20,000 to 800,000 units), preferably 0.1 to 1 g (10,000 to 400,000 units) per 100 g of the substrate protein.
【００１４】中性プロテアーゼによる加水分解処理は、
通常、ｐＨ５〜８、４０〜６０℃、好ましくは５０〜６
０℃、５〜３０時間行う。上記プロテアーゼによる加水
分解処理は、単独で行っても、それらの処理を組み合わ
せて行ってもよい。本発明に用いる小麦またはトウモロ
コシ蛋白質由来加水分解ペプチドは、上記加水分解処理
により取得されるペプチドの他、植物性蛋白ＳＫ−５
（千葉製粉社製）、ペプチーノ（日本食品化工社製）等
の市販されているペプチド鎖長２〜２０、中でも２〜６
個のアミノ酸からなるオリゴペプチドを主成分としてな
る小麦またはトウモロコシ蛋白質由来加水分解ペプチド
を用いることもできる。Hydrolysis treatment with a neutral protease is
Usually pH 5-8, 40-60 ° C, preferably 50-6
Perform at 0 ° C. for 5 to 30 hours. The hydrolysis treatment with the protease may be performed alone or in combination. The hydrolyzed peptides derived from wheat or corn protein used in the present invention include, in addition to the peptides obtained by the above-mentioned hydrolysis treatment, plant protein SK-5.
(Chiba Flour Milling Co., Ltd.), Pepcino (Nippon Shokuhin Kako Co., Ltd.), and other commercially available peptide chain lengths of 2 to 20, especially 2 to 6
It is also possible to use a wheat or corn protein-derived hydrolyzed peptide having an oligopeptide consisting of individual amino acids as a main component.
【００１５】小麦またはトウモロコシ蛋白質由来加水分
解ペプチドを添加する飼料としては、魚粉、オキアミミ
ール、小麦粉、油脂、ビタミン、ミネラル等の粉末原料
にバインダーとしてグルテン、アルファー澱粉と水を加
えてエクストルーダー成形機でペレット状に成形後乾燥
した固形乾燥配合飼料、イワシ、サバ、イカナゴ等の鮮
魚、冷凍魚をミンチにした生餌飼料、および、ペレット
に成形しない上記配合飼料の粉末とミンチ状生餌飼料を
１対１から１対５の比率で混合後、ペレッターで成形し
たモイストペレット飼料を用いることができる。As the feed to which the hydrolyzed peptide derived from wheat or corn protein is added, an extruder molding machine is used in which gluten, alpha starch and water are added as binders to powder raw materials such as fish meal, krill meal, wheat flour, fats and oils, vitamins and minerals. Solid dry blended feed molded into pellets and dried with fresh fish such as sardines, mackerel, lizard, minced frozen fish, and powder of the above compounded feed not minced into pellets and minced raw feed. After mixing at a ratio of 1: 1 to 1: 5, a moist pellet feed molded by a pelleter can be used.
【００１６】小麦またはトウモロコシ蛋白質由来加水分
解ペプチドの投与量としては、通常用いられる飼料に
０．０１〜１％の割合で添加し、養殖魚体重１ｋｇあた
りペプチドとして１０ｍｇ〜１０００ｍｇ／日、好まし
くは２５ｍｇ〜５００ｍｇ／日の量を投与する。投与方
法は、所定量のペプチドを添加した飼料を、魚体重の１
〜１０％の割合で、連日、または隔日、または週５日等
の間歇投与を行う。The hydrolyzed peptide derived from wheat or corn protein is added to a commonly used feed in a proportion of 0.01 to 1%, and 10 mg to 1000 mg / day, preferably 25 mg, of peptide per 1 kg of cultured fish weight. Dosage of ~ 500 mg / day. The administration method was as follows.
The intermittent administration is performed at a rate of -10% every day, every other day, or 5 days a week.
【００１７】[0017]
【実施例】以下、実施例をあげて本発明を具体的に説明
する。 〔実施例１〕イワシミンチ５０部と粉末ブリ用配合飼料
（マルハ社製ニューハマチモイストＳ）５０部を混合し
て合計１００部としたものに、小麦蛋白質（グルテン）
１％または後記参考例１で取得した小麦蛋白質酵素加水
分解物（以下「小麦ペプチド」と称する）、およびトウ
モロコシペプチド「ペプチーノ」（トウモロコシの蛋白
を酵素加水分解して得られたペプチド：日本食品化学社
製）を外割りでそれぞれ０．０１％、０．１％、１％を
加えた混合飼料を作製した。モイストペレッター（サン
コーテクノ社製）を用いて、該混合飼料から表１に示し
たモイスト飼料を作製した。モイストペレットの形状
は、径５ｍｍ、長さ１０ｍｍとした。The present invention will be described below in detail with reference to examples. [Example 1] Wheat protein (gluten) was added to 50 parts of sardines minced and 50 parts of powdered yellowtail compound feed (New Hamachi Moist S manufactured by Maruha Co., Ltd.) to make a total of 100 parts.
1% or enzymatically hydrolyzed wheat protein (hereinafter referred to as "wheat peptide") obtained in Reference Example 1 below, and corn peptide "pepticino" (peptide obtained by enzymatically hydrolyzing corn protein: Nippon Food Chemistry) (Manufactured by the company) was mixed with 0.01%, 0.1%, and 1% to prepare mixed feeds. The moist feed shown in Table 1 was prepared from the mixed feed using a moist pelleter (manufactured by Sanko Techno Co., Ltd.). The shape of the moist pellets was 5 mm in diameter and 10 mm in length.
【００１８】[0018]
【表１】 [Table 1]
【００１９】平均体重約１００ｇのブリ幼魚１６０尾を
２０尾づつの８群に分けて実験室内に設置された１ｍ3
円形プラスチック水槽８基に収容し、それぞれ試験区−
１、２、３、４、５、６、７および８とした。試験区−
１には表１に示した試験飼料−１を、試験区−２には飼
料−２を、試験区−３には飼料−３を、試験区−４には
飼料−４を、試験区−５には飼料−５を、試験区−６に
は飼料−６を、試験区−７には飼料−７、試験区−８に
は飼料−８をそれぞれ給餌して３週間の飼育を行った。
給餌量は供試魚体重の約５％を投与した。従って魚体重
１ｋｇあたりのペプチーノ投与量は、それぞれ試験区−
３と試験区−６では５ｍｇ／日、試験区−４と試験区−
７では５０ｍｇ／日、試験区−５と試験区−８では５０
０ｍｇ／日となった。1m 3 of 160 juvenile yellow-tailed fish with an average weight of about 100 g were divided into 8 groups of 20 fish each and placed in the laboratory.
It is housed in 8 round plastic water tanks, and
1, 2, 3, 4, 5, 6, 7, and 8. Test area-
1, the test feed-1 shown in Table 1, the test plot-2, the feed-2, the test plot-3, the feed-3, the test plot-4, the feed-4, and the test plot- 5 was fed with feed-5, test plot-6 was fed with feed-6, test plot-7 was fed with feed-7, and test plot-8 was fed with feed-8, respectively, and raised for 3 weeks. .
About 5% of the body weight of the test fish was administered. Therefore, the dose of pepticino per 1 kg of fish weight was
5 mg / day in 3 and test section-6, test section-4 and test section-
50 mg / day for 7 and 50 for test plots 5 and 8
It became 0 mg / day.
【００２０】飼育試験３週間経過後、各試験区に対して
連鎖球菌症原因菌による感染攻撃試験を実施した。感染
攻撃試験方法は、ブレインハートインフュージョン液体
培地で２５℃、２４時間培養したブリ連鎖球菌症原因菌
（Enterococcus seriolicida）菌体を表１に示した試験
飼料−１に添加して経口投与した。投与量は試験魚１尾
あたり生菌数として３×１０8cells／日の量を３日間投
与した。After 3 weeks from the breeding test, an infection attack test with streptococcal causative bacteria was carried out on each test plot. The infection attack test method was carried out by orally administering the Streptococcus erieriocida ( Enterococcus seriolicida ) cells cultured in a brain heart infusion liquid medium at 25 ° C. for 24 hours to the test feed-1 shown in Table 1. The dose was 3 × 10 8 cells / day as the viable cell count per test fish, which was administered for 3 days.
【００２１】菌投与終了後は再び上記試験飼料をそれぞ
れの試験区に投与して２週間飼育を続け、その間の斃死
状態を観察した。水温２５±１℃、換水率１０回／日の
流水飼育条件下で実施した。試験結果を表２に示した。After the administration of the bacteria, the above test feed was again administered to each test section and the breeding was continued for 2 weeks, and the mortality during the period was observed. Water temperature was 25 ± 1 ° C., and the water exchange rate was 10 times / day. The test results are shown in Table 2.
【００２２】[0022]
【表２】 [Table 2]
【００２３】〔実施例２〕１０ｍ×１０ｍ×８ｍの網生
簀３０基が設置されているブリ養殖漁場の中で一養殖生
産者が所有する３基の網生簀を対象に小麦ペプチドの投
与試験を実施した。魚体重約７５０ｇのブリがそれぞれ
８０００尾ずつ収容された網生簀３基に、冷凍イワシ２
部に対しハマチ用マッシュ（マルハ社製）１部からなる
モイストペレットよりなる通常飼料、通常飼料に小麦蛋
白質（グルテン）を０．１％添加した飼料あるいは通常
飼料に小麦ペプチドを０．１％添加した飼料のいずれか
を、一日当たりそれぞれ魚体重の４〜５％の量になるよ
うに投与した。[Example 2] A wheat peptide administration test was conducted on three net cages owned by one aquaculture producer in a yellowtail aquaculture fishery where 30 net cages measuring 10 m x 10 m x 8 m were installed. Carried out. Two sardines with three fish cages each containing 8,000 fish weighing about 750g and two frozen sardines
Oral feed consisting of moist pellets consisting of 1 part of mash for malt (Maruha Co., Ltd.), feed containing 0.1% wheat protein (gluten) in normal feed or 0.1% wheat peptide in normal feed Each of the prepared diets was administered in an amount of 4 to 5% of fish body weight per day.
【００２４】試験は８月１日により９月３０日までの２
ケ月間実施し、その間の病害発生状況、斃死状況を観察
した。結果を表３に示した。The test is from August 1st to September 30th 2
It was carried out for a month, and the occurrence of diseases and deaths during that period were observed. The results are shown in Table 3.
【００２５】[0025]
【表３】 [Table 3]
【００２６】試験期間中、連鎖球菌症が発生し、通常飼
料および小麦蛋白質を添加した試験区では一生簀当たり
一日平均１．７〜２．４尾の斃死が見られたが、小麦ペ
プチドを投与した試験区では、一生簀当たり一日平均
１．０尾で、対照区に比べ１／２の斃死で推移し、本疾
病に対する抵抗性が見られた。また、９月３０日に試験
を実施していた海域に低酸素水塊が出現し、同海域の養
殖ブリが大量斃死をする被害を受け、試験実施中の生簀
でも大量斃死が見られた。During the test period, streptococcal disease occurred, and in the test group to which normal feed and wheat protein were added, death of 1.7 to 2.4 fish per day was observed on average per day, but wheat peptide was added. In the administered test group, the average of 1.0 fish per day in the cage was dying half that in the control group, and resistance to this disease was observed. In addition, a hypoxic water mass appeared in the sea area where the test was conducted on September 30, causing a large number of deaths of the cultured yellowtail in the sea area, and a large number of deaths were also seen in the cage during the test.
【００２７】通常飼料および小麦蛋白質を添加した試験
区の生簀では、それぞれ１８００尾と１７５０尾の斃死
魚があったが、小麦ペプチドを投与していた試験区の生
簀では１８０尾の斃死で、対照区の１／１０にとどまっ
た。この結果より、小麦ペプチドを投与することによ
り、漁場環境悪化に対する養殖ブリの抵抗性が著しく増
強されたものと考えられた。There were 1800 and 1750 dead fish in the cages of the test group to which the normal feed and wheat protein were added, respectively, but 180 cages were killed in the cage of the test group to which the wheat peptide had been administered, which was the control. It stayed at 1/10 of the ward. From this result, it was considered that the resistance of the cultured yellowtail to the deterioration of the fishing ground environment was significantly enhanced by the administration of the wheat peptide.
【００２８】〔参考例１〕 小麦蛋白質酵素加水分解物
の製造 小麦蛋白質（グルテン）４０ｋｇを４００Ｌの水に懸濁
し、希塩酸添加によりｐＨ５．５に調整し、生澱粉分解
酵素「ダビアーゼ」（ダイキン工業株式会社製）５０ｇ
を添加し、５０℃で５時間反応させた後、フィルタープ
レスにて固液分離し、小麦蛋白質のウエットケーキ３５
ｋｇを得た。このウエットケーキを蒸留水３５０Ｌに再
懸濁させ、水酸化ナトリウムを添加してｐＨ１２に調整
し、アルカリ性プロテアーゼ・アルカラーゼ（ノボイン
ダストリージャパン社製）８０ｇを添加し、ｐＨ９．０
に調整しつつ２０時間反応させた。反応後、硫酸または
塩酸でｐＨ５．５に中和した後、フィルタープレスにて
固液分離して酵素加水分解物を得た。Reference Example 1 Production of Wheat Protein Enzyme Hydrolyzate 40 kg of wheat protein (gluten) was suspended in 400 L of water, and the pH was adjusted to 5.5 by adding dilute hydrochloric acid, and the raw starch degrading enzyme “Daviase” (Daikin Industry Co., Ltd.) was used. 50g)
Was added and reacted at 50 ° C. for 5 hours, followed by solid-liquid separation with a filter press to obtain a wet cake of wheat protein 35
I got kg. This wet cake was resuspended in 350 L of distilled water, sodium hydroxide was added to adjust the pH to 12, 80 g of alkaline protease / alcalase (manufactured by Novo Industry Japan) was added, and pH was 9.0.
It was made to react for 20 hours while adjusting to. After the reaction, the mixture was neutralized to pH 5.5 with sulfuric acid or hydrochloric acid, and then solid-liquid separated with a filter press to obtain an enzymatic hydrolyzate.
【００２９】該加水分解物をイオン交換樹脂にて脱塩、
脱色、脱臭、活性炭処理および加熱殺菌の後乾燥して白
色〜淡黄色粉末である小麦蛋白質酵素加水分解物「小麦
ペプチド」を得た。該小麦ペプチドのアミノ酸組成を表
４に、Shodex OHpak KB802.5カラムを用いたＨＰＬＣパ
ターンを図１に示した。ＨＰＬＣは以下の条件で行っ
た。The hydrolyzate is desalted with an ion exchange resin,
Decolorization, deodorization, activated carbon treatment, heat sterilization and drying were performed to obtain a wheat protein enzyme hydrolyzate "wheat peptide" which was a white to pale yellow powder. The amino acid composition of the wheat peptide is shown in Table 4, and the HPLC pattern using a Shodex OHpak KB802.5 column is shown in FIG. HPLC was performed under the following conditions.
【００３０】 カラム：Shodex OHpak KB802.5 移動相：アセトニトリル：水：酢酸＝４０：５９．９：１（Ｖ／Ｖ） 流速 ：０．５ｍｌ／ｍｉｎ 圧力 ：３０ｋｇ／ｍ2 温度 ：室温 検出器：HITACHI UV monitor L-4000,280nm データ処理器：HITACHI D-2500Column: Shodex OHpak KB802.5 Mobile phase: Acetonitrile: Water: Acetic acid = 40: 59.9: 1 (V / V) Flow rate: 0.5 ml / min Pressure: 30 kg / m 2 Temperature: Room temperature Detector: HITACHI UV monitor L-4000,280nm Data processor: HITACHI D-2500
【００３１】[0031]
【表４】 [Table 4]
【００３２】[0032]
【発明の効果】本発明によれば、養殖魚の連鎖球菌症
等、その病原菌進入部位が主として消化管である細菌性
疾病に対する抗病性の向上、および低酸素水塊等の環境
悪化に対する抵抗性を向上させ、これ等養殖魚の生残率
の向上および成育を促進させるための飼料を提供するこ
とができる。INDUSTRIAL APPLICABILITY According to the present invention, improved resistance to bacterial diseases such as streptococcal disease of farmed fish whose pathogen entry site is mainly the digestive tract, and resistance to environmental deterioration such as low oxygen water mass. It is possible to provide a feed for improving the survival rate, improving the survival rate and promoting the growth of these cultured fish.
【図１】参考例１で得られたペプチド組成物を高速液体
クロマトグラフィー（ＨＰＬＣ）にかけたときの溶出曲
線を示す図である。FIG. 1 is a diagram showing an elution curve when the peptide composition obtained in Reference Example 1 was subjected to high performance liquid chromatography (HPLC).
１…アルブミン（分子量43,000） ２…リボヌクレアーゼＡ（分子量13,700） ３…チロシン（分子量187）およびフェニルアラニン
（分子量165）1 ... Albumin (molecular weight 43,000) 2 ... Ribonuclease A (molecular weight 13,700) 3 ... Tyrosine (molecular weight 187) and phenylalanine (molecular weight 165)
───────────────────────────────────────────────────── フロントページの続き (72)発明者 松浦 一郎 東京都板橋区中台３−27 Ｋ−212 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Ichiro Matsuura 3-27 Nakadai, Itabashi-ku, Tokyo K-212
Claims (4)
加水分解して得られるオリゴペプチドを含有してなる養
殖魚用飼料。1. A feed for cultured fish comprising an oligopeptide obtained by hydrolyzing wheat protein or corn protein.
０のオリゴペプチドである請求項１記載の養殖魚用飼
料。2. The oligopeptide has a peptide chain length of 2 to 2.
The feed for cultured fish according to claim 1, which is an oligopeptide of No. 0.
のオリゴペプチドである請求項１記載の養殖魚用飼料。3. The oligopeptide has a peptide chain length of 2 to 6
The feed for cultured fish according to claim 1, which is the oligopeptide of
ウモロコシ蛋白質をを、好アルカリ性細菌由来のアルカ
リ性プロテアーゼにより加水分解することにより得られ
るオリゴペプチドである請求項１〜３いずれかに記載の
養殖魚用飼料。4. The feed for farmed fish according to claim 1, wherein the oligopeptide is an oligopeptide obtained by hydrolyzing wheat protein or corn protein with an alkaline protease derived from an alkalophilic bacterium. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7242853A JPH0984528A (en) | 1995-09-21 | 1995-09-21 | Feed for pisciculture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7242853A JPH0984528A (en) | 1995-09-21 | 1995-09-21 | Feed for pisciculture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0984528A true JPH0984528A (en) | 1997-03-31 |
Family
ID=17095253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7242853A Withdrawn JPH0984528A (en) | 1995-09-21 | 1995-09-21 | Feed for pisciculture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0984528A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009284835A (en) * | 2008-05-30 | 2009-12-10 | Fuji Oil Co Ltd | Green liver syndrome-preventive fish feed |
| CN105076831A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Compound feed for juvenile fish of channa maculate and preparation method of compound feed |
| CN105076830A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Compound feed for channa maculate and preparation method of compound feed |
-
1995
- 1995-09-21 JP JP7242853A patent/JPH0984528A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009284835A (en) * | 2008-05-30 | 2009-12-10 | Fuji Oil Co Ltd | Green liver syndrome-preventive fish feed |
| CN105076831A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Compound feed for juvenile fish of channa maculate and preparation method of compound feed |
| CN105076830A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Compound feed for channa maculate and preparation method of compound feed |
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