JPH0967262A - Skin activator and skin activating food - Google Patents
Skin activator and skin activating foodInfo
- Publication number
- JPH0967262A JPH0967262A JP7248631A JP24863195A JPH0967262A JP H0967262 A JPH0967262 A JP H0967262A JP 7248631 A JP7248631 A JP 7248631A JP 24863195 A JP24863195 A JP 24863195A JP H0967262 A JPH0967262 A JP H0967262A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- skin
- component
- tochu
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、杜仲若しくはその
抽出物及び人参若しくはその抽出物を必須成分とする皮
膚賦活剤及び皮膚賦活食品、さらに詳しくは、生体の新
陳代謝を促進し、特に皮膚のターンオーバーを改善する
皮膚賦活剤及び皮膚賦活食品に関する。TECHNICAL FIELD The present invention relates to a skin activating agent and a skin activating food containing Tochu or an extract thereof and ginseng or an extract thereof as an essential component, and more particularly to promoting the metabolism of a living body, and in particular, turning the skin. The present invention relates to a skin activating agent and a skin activating food for improving overcoat.
【０００２】[0002]
【従来の技術】通常若い肌は、新陳代謝を活発に行い、
疲労、機能低下等を素早く改善する。しかしながら、加
齢変化に伴い、組織の新陳代謝は低下し、特に、皮膚の
ターンオーバー期間が長くなり、組織疲労と萎縮、酸化
が進むことにより本来の機能が低下する現象が観察され
ている。この新陳代謝の低下により健康が害されるおそ
れがあり、各種疾病にかかりやすく、また疾病からの回
復を遅らせてしまう。さらに、新陳代謝の低下により肌
のハリの低下、小ジワ、くすみ等の問題を生じる。そこ
で、この新陳代謝の低下を改善する対応策が考慮されて
いる。2. Description of the Related Art Normally, young skin actively undergoes metabolism,
Quickly improve fatigue and functional decline. However, it has been observed that with age-related changes, the metabolism of tissues declines, and in particular, the turnover period of the skin becomes longer, and tissue fatigue, atrophy, and oxidation progress, leading to a decline in the original function. This decrease in metabolism may impair health, is prone to various diseases, and delays recovery from the diseases. Furthermore, the decrease in metabolism causes problems such as a decrease in skin firmness, fine lines and dullness. Therefore, countermeasures for improving this decrease in metabolism are being considered.
【０００３】これを改善するため現在上市されている商
品の一つに、コラーゲンと杜仲葉とＤＮＡ核酸とカルシ
ウムと各種ビタミンを配合したものがある。また、これ
にさらにフラクトオリゴ糖を加えたものもある。これら
を食生活中に取り入れることにより新陳代謝を促進する
というものである。また、新陳代謝を促進する組成物に
関し、特開平１−１０８９６３号公報で開示されたもの
がある。これは、朝鮮人参と杜仲葉若しくはそれらの抽
出物からになり、これを飲用することにより新陳代謝が
促進されるというものである。しかしながら、この組成
物においては、朝鮮人参の配合により尿の出が悪くなる
という欠点が報告されている。In order to improve this, one of the products currently on the market is one containing collagen, Tochu leaf, DNA nucleic acid, calcium and various vitamins. Further, there is also one in which fructooligosaccharide is further added. By incorporating these in the diet, it is intended to promote metabolism. Further, regarding a composition that promotes metabolism, there is one disclosed in JP-A-1-108963. It consists of ginseng and Tochu leaf or their extracts, and its metabolism promotes its metabolism. However, it has been reported that this composition has a drawback in that urine output becomes poor due to the incorporation of ginseng.
【０００４】さらに、第４８回日本栄養・食糧学会で目
鳥らによりコラーゲン分解物と、杜仲葉とを含む低蛋白
質食品がラットの肉芽形成を促進する効果がある旨報告
されている。本報告によれば、低蛋白質食に杜仲葉と人
参を混合した飼料を４週与えたラットの一部に、４〜５
週の１週間蛋白質に代えて分解コラーゲンを与えたとこ
ろ、低蛋白質食を５週続けたラットよりも肉芽量が多
く、さらに組織中のヒドロキシプロリンの含量も多く、
高蛋白質食を続けたラットと同程度の肉芽形成促進能が
あることが報告されている。しかも、高蛋白質食を続け
たラットと比較して体重の増加は少なく、低蛋白質食を
続けたラットと同程度の体重増加しか示さなかった。し
かるに、これを人間の一般生活に当てはめた場合には、
コラーゲンと杜仲と人参を同時に摂取することにより、
良好に新陳代謝の促進が図れることが示唆される。Furthermore, it has been reported at the 48th Japan Society of Nutrition and Food Science that low-protein foods containing collagen hydrolysates and Tochu leaf have an effect of promoting granulation in rats. According to this report, some rats fed a low-protein diet mixed with Tochu-leaf and carrot for 4 weeks had 4-5
When degrading collagen was given instead of protein for 1 week, the amount of granulation was higher than that of the rat that continued a low protein diet for 5 weeks, and the content of hydroxyproline in the tissue was also higher.
It has been reported that it has the same ability to promote granulation as that of a rat that has continued on a high protein diet. Moreover, the increase in body weight was less than that of the rats that continued on the high protein diet, and the weight gain was similar to that of the rats that continued on the low protein diet. However, if this is applied to human general life,
By simultaneously ingesting collagen, Tochu and carrot,
It is suggested that metabolism can be promoted well.
【０００５】[0005]
【発明が解決しようとする課題】しかしながら、上記組
成の新陳代謝促進剤を調製したところで、組織合成促進
は図れるものの、皮膚のターンオーバーは十分促進でき
ず、肌のハリの低下、小ジワ、くすみ等の問題は未だ解
決されず、さらなる改良が望まれている。本発明は、蛋
白質を通常の食生活以上に摂取する必要がなく、しかも
新陳代謝を促進し、特に皮膚のターンオーバーを促進す
る皮膚賦活剤及び皮膚賦活食品を開発することを目的と
する。However, when the metabolism enhancer having the above composition is prepared, the tissue synthesis can be promoted, but the turnover of the skin cannot be sufficiently promoted, and the firmness of the skin is reduced, fine lines, dullness, etc. The problem of is not solved yet, and further improvement is desired. It is an object of the present invention to develop a skin activating agent and a skin activating food that do not require ingestion of protein more than usual dietary habits, and promote metabolism, especially skin turnover.
【０００６】[0006]
【課題を解決するための手段】前記目的を解決するため
に本発明者らが鋭意検討を重ねた結果、杜仲と人参とを
必須成分とし、デオキシリボ核酸等の選択成分を含有す
る皮膚賦活剤を用いることにより、該成分が皮膚の新陳
代謝を促進し、組織合成能が高まり、皮膚のターンオー
バーを促進し、皮膚賦活剤として有効に機能することを
見出し本発明の完成に至った。すなわち、本発明の皮膚
賦活剤は、杜仲若しくはその抽出物と、人参若しくはそ
の抽出物とを必須成分とし、これにデオキシリボ核酸、
コンドロイチン硫酸、コラーゲン若しくはその加水分解
物、ハトムギエキスの一種又は二種以上を選択して配合
することを特徴とする。また、本発明の皮膚賦活食品
は、前記皮膚賦活剤を含有することを特徴とする。Means for Solving the Problems As a result of intensive studies by the present inventors in order to solve the above-mentioned object, a skin activating agent containing Euglena and carrot as essential components and a selective component such as deoxyribonucleic acid was selected. It has been found that the use of these components promotes the metabolism of the skin, enhances the tissue synthesizing ability, promotes the turnover of the skin, and effectively functions as a skin activating agent, thereby completing the present invention. That is, the skin activating agent of the present invention contains Tochu or its extract and ginseng or its extract as essential components, and deoxyribonucleic acid,
One or more chondroitin sulfates, collagens or hydrolysates thereof, and adlay extracts are selected and blended. The skin activating food of the present invention is characterized by containing the skin activating agent.
【０００７】[0007]
【発明の実施の形態】以下、本発明の実施形態をさらに
詳細に説明する。本発明は必須成分として、杜仲及び人
参若しくはこれらの抽出物を含む。本発明に用いられる
杜仲は、杜仲の葉をそのまま若しくは杜仲エキスを抽出
した抽出物として用いる。DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in more detail. The present invention includes Tochu and ginseng or extracts thereof as essential components. As the Tochu used in the present invention, the Tochu leaf is used as it is or as an extract obtained by extracting the Tochu extract.
【０００８】すなわち、杜仲の葉をそのまま用いる場合
には、細かく裁断したものをそのまま、若しくはそれを
乾燥させる、若しくは前記粉砕した杜仲の葉を常法に従
い、例えば８０〜１２０℃で０．５〜２時間焙煎して得
たものを用いることが可能である。また、杜仲抽出物と
して用いる場合には、杜仲の葉をそのまま若しくはその
乾燥物を常法に従い、例えば水あるいは水性有機溶剤に
て、室温あるいは８０〜１００℃にて抽出して得られた
抽出液を濾過後、そのまま、若しくは必要に応じて濃縮
し、若しくは乾燥して粉末として用いることが可能であ
る。また、必要に応じては、発酵処理を行った後、焙
煎、濾過、濃縮等を経て得ることも可能である。That is, when the Tochu leaves are used as they are, finely chopped ones are dried as they are, or the crushed Tochu leaves are subjected to a conventional method, for example, at 0.5 to 80 at 120 to 120 ° C. It is possible to use the one obtained by roasting for 2 hours. When used as a Tochu extract, an extract obtained by extracting Tochu leaves as they are or a dried product thereof according to a conventional method, for example, with water or an aqueous organic solvent at room temperature or 80 to 100 ° C. Can be used as a powder after filtration, as it is, or if necessary, concentrated or dried. In addition, if necessary, it can be obtained by carrying out a fermentation treatment, followed by roasting, filtration, concentration and the like.
【０００９】また、本発明に用いられる人参は、朝鮮人
参をそのまま若しくは人参エキスを抽出した抽出物とし
て用いる。朝鮮人参は上記杜仲と同様に処理し、本発明
に用いることが可能である。さらに本発明は、上記必須
成分に加えて、選択成分としてデオキシリボ核酸、コン
ドロイチン硫酸、コラーゲン若しくはその加水分解物、
ハトムギエキスの一種若しくは二種以上を選択して配合
する。デオキシリボ核酸は、サケ、イワシ、フグ、サバ
等の白子に、コンドロイチン硫酸はフカヒレ等に多量に
含まれている。なお本発明は、上記必須成分に加えて選
択成分の一種または二種以上を配合することにより本発
明の特徴的効果を初めて発揮することができるのであ
る。The ginseng used in the present invention may be ginseng as it is or as an extract obtained by extracting a ginseng extract. Ginseng can be treated in the same manner as the above Tochu and can be used in the present invention. Furthermore, the present invention, in addition to the above essential components, deoxyribonucleic acid, chondroitin sulfate, collagen or a hydrolyzate thereof as a selective component,
One or more kinds of coix extract are selected and blended. Deoxyribonucleic acid is contained in a large amount of salmon, sardines, puffer fish, mackerel, etc., and chondroitin sulfate is contained in shark fins, etc. in large amounts. The present invention can exhibit the characteristic effects of the present invention for the first time by blending one or more selected components in addition to the above essential components.
【００１０】本発明の皮膚賦活剤及び皮膚賦活食品を用
いる場合には、通常摂取者が一日に杜仲若しくはその抽
出物（以下、杜仲という）０．０２〜３ｇ／ｋｇ、人参
若しくはその抽出物（以下、人参という）０．００５〜
１．５ｇ／ｋｇとすることが好ましい。杜仲０．０２ｇ
未満、人参０．００５ｇ未満では、本発明の目的とする
効果を得ることができず、一方、杜仲３ｇ、人参１．５
ｇ以上としてもそれ以上の効果を望めない。また、本発
明の皮膚賦活剤及び皮膚賦活食品中の選択成分の総配合
量は、必須成分である杜仲及び人参の総量に対して、
２：１〜１：１の範囲で配合することが好ましい。When the skin activating agent and the skin activating food of the present invention are used, the ingestor usually takes 0.02 to 3 g / kg of Tochu or its extract (hereinafter referred to as Tochu), ginseng or its extract. (Hereinafter referred to as carrot) 0.005
It is preferably set to 1.5 g / kg. Toshinaka 0.02g
If less than 0.005 g of ginseng, the desired effect of the present invention cannot be obtained, while on the other hand 3 g of Tochu and 1.5 g of ginseng
Even if it is over g, no further effect can be expected. Further, the total amount of the selected components in the skin activating agent and the skin activating food of the present invention is, relative to the total amount of the essential ingredients, Tochu and carrot,
It is preferable to mix in the range of 2: 1 to 1: 1.
【００１１】本発明の皮膚賦活剤は、経口投与で適用す
ることが好ましく、散剤、顆粒、錠剤、カプセル剤、菓
子や清涼飲料水や主食等の食品等主種の使用形態をとる
ことができ、食品に配合することにより皮膚賦活食品と
することが可能である。さらに、本発明の皮膚賦活食品
には上記必須成分、選択成分の他、蛋白質、ローヤルゼ
リー、ムコ多糖類などの動物性抽出物、ビタミン類、不
飽和脂肪酸、等の従来公知の皮膚賦活食品を配合するこ
とも可能である。さらに、本発明の効果を損なわない範
囲で、必要に応じて食品に用いられる賦形剤、増量剤、
甘味剤、香味剤、着色剤等の添加剤を配合することも可
能である。The skin activating agent of the present invention is preferably applied orally and can be used in the form of powders, granules, tablets, capsules, main ingredients such as confectionery, soft drinks and foods such as staple foods. It is possible to make a skin-activating food by mixing it with food. Furthermore, in addition to the above-mentioned essential components and selected components, the skin activating food of the present invention contains proteins, royal jelly, animal extracts such as mucopolysaccharides, vitamins, unsaturated fatty acids, and other conventionally known skin activating foods. It is also possible to do so. Further, as long as the effect of the present invention is not impaired, if necessary, an excipient used in foods, a bulking agent,
It is also possible to add additives such as a sweetening agent, a flavoring agent and a coloring agent.
【００１２】組織合成能の回復 本発明者は本発明のラット肉芽形成に及ぼす影響につい
て検討した。まず、ホルマリン濾紙法（ＦＦＰ法：A.Ta
naka et al, Endocrinol.Japan.1960(4),357〜364）に
より評価を行った。まず、実験方法について説明する。
本実験は、Ｒａｏらの報告（Rao et al, Leather Scien
ce, Vol.33(1),1986,1〜7）をもとに、長期間低蛋白質
食で飼育することによって新陳代謝を大きく低下させた
ラットにおける組織合成能の回復に対する本発明の影響
を検討したものである。 Recovery of tissue synthesizing ability The present inventor examined the effect of the present invention on rat granulation. First, formalin filter paper method (FFP method: A.Ta
naka et al, Endocrinol. Japan. 1960 (4), 357-364). First, the experimental method will be described.
This experiment was reported by Rao et al. (Rao et al, Leather Scien
ce, Vol. 33 (1), 1986, 1-7), and examined the effect of the present invention on the recovery of tissue synthesis ability in rats whose metabolism was significantly reduced by feeding on a low-protein diet for a long period of time. It was done.
【００１３】（１）実験動物 ６週齢のＷｉｓｔｅｒ系雄ラット２０匹を、一週間の予
備飼育後、４群に分け一群５匹とした。 （２）飼料 各群に表１に示す飼料及び飲料水を自由に与えた。(1) Experimental animals Twenty six-week-old Wister male rats were preliminarily reared for one week and divided into four groups to give five rats per group. (2) Feed Each group was given the feed and drinking water shown in Table 1 freely.
【表１】 ──────────────────────────────────── １群 ２群 ３群 ４群 ──────────────────────────────────── ６％蛋白質含有実験食 100.0% 98.7% 98.7% 98.7% 調整食ａ − 1.3% − − 調整食ｂ − − 1.3% − 調整食ｃ − − − 1.3% ──────────────────────────────────── 各調整食１．３％の組成を以下に示す。なお、配合量は
ｍｇで示す。また、６％蛋白質含有実験食の組成は後述
する。[Table 1] ──────────────────────────────────── 1 group 2 groups 3 groups 4 groups ── ────────────────────────────────── 6% Protein-containing experimental food 100.0% 98.7% 98.7% 98.7% Adjusted food a − 1.3% − − Adjusted food b − − 1.3% − Adjusted food c − − − 1.3% ───────────────────────────── ──────── The composition of each prepared food 1.3% is shown below. The blending amount is shown in mg. The composition of the experimental diet containing 6% protein will be described later.
【００１４】[0014]
【表２】 ──────────────────────────────────── 調整食ａ 調整食ｂ 調整食ｃ ──────────────────────────────────── 杜仲・人参エキス ４３０ ４３０ − コラーゲン ２２０ − ２２０ サケ白子抽出物 １１０ − １１０ フカヒレエキス ９０ − ９０ ハトムギエキス ５０ − ５０ 部分α化澱粉 − ４７０ ４３０ ────────────────────────────────────[Table 2] ──────────────────────────────────── Adjusted diet a Adjusted diet b Adjusted diet c ─ ─────────────────────────────────── Morinaka / Ginseng Extract 430 430-Collagen 220-220 Salmon Shirako Extract 110-110 Shark fin extract 90-90 Coix seed extract 50-50 Partially pregelatinized starch-470 430 ─────────────────────────────── ──────
【００１５】なお、ハトムギエキス５０ｍｇには原生薬
換算で１０００ｍｇのハトムギに相当し、サケ白子抽出
物には、１００ｍｇのデオキシリボ核酸、フカヒレエキ
スには、３０ｍｇのコンドロイチン硫酸が含有される。
また、杜仲・人参エキスは、以下の方法で得ることが可
能である。すなわち、杜仲葉及び人参をそれぞれ１０倍
量の水で熱時抽出する。これを濾過後、それぞれを乾燥
し粉末とした。杜仲エキス及び人参エキスを杜仲：人参
＝３：１となるように混合し、かつこの混合エキスを４
３０ｍｇが杜仲葉１．５ｇ、人参０．５ｇに相当するよ
う調製することにより得た。したがって、杜仲・人参エ
キスには、１．５ｇ相当の杜仲葉と、０．５ｇ相当の人
参が含まれている。50 mg of adlay extract corresponds to 1000 mg of adlay in terms of a crude drug, salmon sardine extract contains 100 mg of deoxyribonucleic acid, and shark fin extract contains 30 mg of chondroitin sulfate.
The Tochu / Ginseng extract can be obtained by the following method. That is, each of Tochu leaf and carrot is extracted with 10 times the amount of water when hot. After filtering this, each was dried and made into powder. Tochu extract and carrot extract are mixed so that Tochu: carrot = 3: 1 and this mixed extract is mixed to 4
It was obtained by preparing 30 mg so as to correspond to 1.5 g of Tochu leaf and 0.5 g of carrot. Therefore, the Tochu / Ginseng extract contains 1.5 g of Tochu leaf and 0.5 g of ginseng.
【００１６】（３）評価 ［ＦＦＰ法］実験開始日より３週間目にエーテル麻酔の
下、７％ホルマリンを２０μｌ染み込ませた直径６ｍ
ｍ、重量８ｍｇの濾紙（TOYO No.126）をラット背部皮
下の４カ所に埋め込んだ。同日より所定の飼料、飲料水
でさらに１週間飼育後、エーテル麻酔下、心臓より採血
して屠殺した。(3) Evaluation [FFP method] Under anesthesia with ether 3 weeks after the start of the experiment, 20 μl of 7% formalin was impregnated with a diameter of 6 m.
A filter paper (TOYO No. 126) having a weight of 8 mg and a weight of 8 mg was embedded at four subcutaneous sites on the back of the rat. From the same day, the animals were further raised for 1 week with prescribed feed and drinking water, and then anesthetized with ether, blood was collected from the heart and killed.
【００１７】上記飼料を用いて実験を行った結果を表３
に示す。なお、＊はStudent's t-test（有意水準p<0.0
5）により統計処理を行った場合に有意差が認められる
ものを示す。The results of experiments conducted using the above feeds are shown in Table 3.
Shown in * Indicates Student's t-test (significance level p <0.0
5) shows a significant difference when statistical processing is performed.
【表３】 ──────────────────────────────────── １群 ２群 ３群 ４群 ──────────────────────────────────── 体重増(g) 71± 7 62± 7 49± 6 76± 5 肉芽湿重量(mg) 152±10 205±14* 189± 7* 152± 7 ────────────────────────────────────[Table 3] ──────────────────────────────────── 1 group 2 groups 3 groups 4 groups ── ────────────────────────────────── Weight gain (g) 71 ± 7 62 ± 7 49 ± 6 76 ± 5 Granulation wet weight (mg) 152 ± 10 205 ± 14 * 189 ± 7 * 152 ± 7 ────────────────────────────── ───────
【００１８】選択成分であるデオキシリボ核酸等のみを
配合した４群では、１群と比較して体重増加及び肉芽組
織合成とも差はなかった。また、実験食に本発明を配合
した２群及び実験食に杜仲・人参を配合した３群では、
体重増加は１群と同程度であった。しかしながら、両群
とも肉芽湿重量において、コントロールとなる１群に対
して有意差が認められ、本発明を配合した２群において
顕著な増加を示した。したがって、杜仲−人参を加える
ことにより肉芽組織合成能が高くなり、さらに、デオキ
シリボ核酸等の選択成分を同時に加えて与えることによ
り、組織合成能の回復をはかることが可能であり、新陳
代謝が促進されることが示唆される。There was no difference in weight gain and granulation tissue synthesis in the 4 groups containing only deoxyribonucleic acid, which was a selective component, as compared with 1 group. In addition, in the 2 groups in which the present invention was added to the experimental diet and the 3 groups in which Tochu and carrot were added to the experimental diet,
Weight gain was similar to group 1. However, in both groups, a significant difference in the wet weight of granulation was observed as compared with the control 1 group, and a marked increase was shown in the 2 groups to which the present invention was added. Therefore, the addition of Tochu-Ginseng enhances the ability to synthesize granulation tissue, and by additionally adding selective components such as deoxyribonucleic acid, it is possible to restore the ability to synthesize tissue and promote metabolism. It is suggested that
【００１９】皮膚のターンオーバーの回復 次に、ダンシルクロライド法による皮膚のターンオーバ
ーの検討を行った。 （１）実験動物 ６週齢のＷｉｓｔａｒ系雄ラット２０匹を１週間の予備
飼育後、４群に分け１群５匹とした。なお、各群の動物
はダンシルクロライド塗布３日前より１匹飼いとした。 Recovery of Skin Turnover Next, skin turnover was examined by the dansyl chloride method. (1) Experimental animal Twenty six-week-old male Wistar rats were preliminarily reared for one week, and then divided into four groups to give five rats per group. The animals in each group were kept one from 3 days before the application of dansyl chloride.
【００２０】（２）飼料 各群に表４に示す飼料及び飲料水は自由に与えた。(2) Feed The feed and drinking water shown in Table 4 were freely given to each group.
【表４】 ──────────────────────────────────── １群 ２群 ３群 ４群 ──────────────────────────────────── １１％蛋白質含有実験食 100.0% 97.0% 97.0% 97.0% 調整食ａ − 3.0% − − 調整食ｂ − − 3.0% − 調整食ｃ − − − 3.0% ──────────────────────────────────── 各調整食は、表２に示す物を用いた。なお、１１％蛋白
質含有実験食については後述する。[Table 4] ──────────────────────────────────── 1 group 2 groups 3 groups 4 groups ── ────────────────────────────────── 11% Protein-containing experimental food 100.0% 97.0% 97.0% 97.0% Adjusted food a − 3.0% − − Adjusted food b − − 3.0% − Adjusted food c − − − 3.0% ───────────────────────────── ──────── The ingredients shown in Table 2 were used as the prepared foods. The experimental diet containing 11% protein will be described later.
【００２１】（３）評価 各動物につき、体重、各臓器の重量及び、ダンシルクロ
ライド法による皮膚の蛍光消失日を測定した。 ［体重及び各臓器の重量の測定］実験開始日より６週間
目にエーテル麻酔下で、心臓より採血し屠殺した。 ［ダンシルクロライド法の蛍光消失日の測定］実験開始
日より各群の動物に飼料及び飲料水を自由に与えた。実
験開始日より４週間目に、エーテル麻酔下、ラットの背
部を剃毛し、２％ダンシルクロライド／ＥｔＯＨ溶液５
μｌをラット背部（ラット自身でふれることができない
部分）に塗布した。塗布後、２４時間目より蛍光強度を
測定し、蛍光の５０％消失日を算定した。なお、各統計
処理は、Duncan Multipl testを用いて有意水準をp<0.0
5として対照群（１群）と比較解析した。(3) Evaluation For each animal, the weight, the weight of each organ, and the day on which skin fluorescence disappeared by the dansyl chloride method were measured. [Measurement of Body Weight and Weight of Each Organ] Six weeks after the start of the experiment, blood was collected from the heart under ether anesthesia and sacrificed. [Measurement of fluorescence disappearance date by dansyl chloride method] From the start date of the experiment, animals in each group were provided with feed and drinking water ad libitum. Four weeks after the start of the experiment, the rat's back was shaved under ether anesthesia and 2% dansyl chloride / EtOH solution 5
μl was applied to the back of the rat (the part where the rat itself cannot touch). The fluorescence intensity was measured from the 24th hour after the application, and the day when 50% of the fluorescence disappeared was calculated. For each statistical processing, the Duncan Multipl test was used to set the significance level to p <0.0.
5 was compared and analyzed with the control group (1 group).
【００２２】まず、各群の体重変化を表５に示す。First, Table 5 shows changes in body weight of each group.
【表５】 ──────────────────────────────────── 開始日の体重Ａ（ｇ） 最終日の体重Ｂ（ｇ） Ｂ−Ａ（ｇ） ──────────────────────────────────── １群 250 ± 3 388 ± 7 138 ± 6 ２群 249 ± 4 378 ± 6 129 ± 3 ３群 246 ± 4 368 ± 7 122 ± 7 ４群 249 ± 6 387 ± 15 138 ± 10 ──────────────────────────────────── この１群と比較して、杜仲−人参及び／またはデオキシ
リボ核酸等を与えた２〜４群はそれぞれ、１２９ｇ、１
２２ｇ、１３８ｇであり、１群との間に有意差は認めら
れなかった。[Table 5] ──────────────────────────────────── Weight on start date A (g) Last day Body weight B (g) BA (g) ───────────────────────────────────── 1st group 250 ± 3 388 ± 7 138 ± 6 2 group 249 ± 4 378 ± 6 129 ± 3 3 group 246 ± 4 368 ± 7 122 ± 7 4 group 249 ± 6 387 ± 15 138 ± 10 ───────── ──────────────────────────── Compared with this one group, Tochu-carrot and / or deoxyribonucleic acid etc. The four groups are 129g and 1 respectively
It was 22 g and 138 g, and there was no significant difference with the 1 group.
【００２３】次に、各群の各臓器の体重に対する割合を
表６に示す。Next, Table 6 shows the ratio of each organ to the body weight of each group.
【表６】 ──────────────────────────────────── 肝臓 腎臓 脾臓 心臓 胸腺 (×102) (×103) (×103) (×103) (×103 ) ──────────────────────────────────── １群 2.85±0.09 5.60±0.20 1.75±0.08 2.65±0.04 1.45±0.10 ２群 2.70±0.06 5.61±0.07 1.67±0.05 2.62±0.03 1.40±0.10 ３群 2.74±0.03 5.62±0.16 1.73±0.08 2.69±0.04 1.38±0.04 ４群 2.74±0.11 5.75±0.27 1.63±0.06 2.70±0.06 1.38±0.06 ──────────────────────────────────── 上記結果より明らかなように、２〜４群の各種臓器の比
体重値には対照群である１群と比較して、全ての臓器に
おいて有意差は認められず、解剖時の肉眼所見によって
も変化は認められなかった。したがって、体重の変化と
各臓器の比体重値を鑑みると、本発明の効果は、ラット
の成長や各種臓器には影響を及ぼさないことが示唆され
る。[Table 6] ──────────────────────────────────── Liver Kidney Spleen Heart Heart Thymus (× 10 2 ) (× 10 3 ) (× 10 3 ) (× 10 3 ) (× 10 3 ) ─────────────────────────────── ────── 1st group 2.85 ± 0.09 5.60 ± 0.20 1.75 ± 0.08 2.65 ± 0.04 1.45 ± 0.10 2nd group 2.70 ± 0.06 5.61 ± 0.07 1.67 ± 0.05 2.62 ± 0.03 1.40 ± 0.10 3rd group 2.74 ± 0.03 5.62 ± 0.16 1.73 ± 0.08 2.69 ± 0.04 1.38 ± 0.04 4 groups 2.74 ± 0.11 5.75 ± 0.27 1.63 ± 0.06 2.70 ± 0.06 1.38 ± 0.06 ─────────────────────────── ────────── As is clear from the above results, there is a significant difference in the specific body weight values of the various organs in groups 2 to 4 in all organs as compared to the control group 1. Was not observed, and no change was observed even by the macroscopic findings at the time of dissection. Therefore, in view of the change in body weight and the specific weight value of each organ, it is suggested that the effect of the present invention does not affect the growth of rats and various organs.
【００２４】次に、各群のダンシルクロライド法による
蛍光消失日の結果を表７に示す。Next, Table 7 shows the results of fluorescence disappearance date of each group by the dansyl chloride method.
【表７】 ──────────────────────────────────── 蛍光の５０％消失日 ──────────────────────────────────── １群 4.73 ±0.41 ２群 3.66 ±0.01* ３群 5.13 ±0.66 ４群 3.95 ±0.41 ──────────────────────────────────── 以上の結果より明らかなように皮膚ターンオーバーに関
しては、蛍光強度の５０％消失日が２群の４．７３日と
比較して、杜仲−人参エキス若しくはデオキシリボ核酸
等を加えた３群及び４群は、蛍光強度の５０％消失日が
５．１３日、３．９５日であり、２群と比較して有意差
は認められない。[Table 7] ──────────────────────────────────── 50% disappearance date of fluorescence ──── ──────────────────────────────── 1 group 4.73 ± 0.41 2 groups 3.66 ± 0.01 * 3 groups 5.13 ± 0.66 4 groups 3.95 ± 0.41 ──────────────────────────────────── As seen from the above results, skin turnover Regarding 50% of the fluorescence intensity disappearance day was 4.73 days in the 2 groups, the 50% disappearance date of the fluorescence intensity was 5% in the 3rd and 4th groups to which Tochu-Ginseng extract or deoxyribonucleic acid was added. 13 days, 3.95 days, showing no significant difference compared with the 2 groups.
【００２５】一方、本発明である杜仲−人参エキスとデ
オキシリボ核酸等を加えて与えた２群では、蛍光強度の
５０％消失日が３．６６日と、２群と比べ有意に消失速
度が速く、新陳代謝が活発化され、しかもターンオーバ
ー期間が短くなっていることが示唆される。したがっ
て、本発明を人間に適用した場合、新陳代謝を促進し、
特に皮膚のターンオーバーを促進することが可能であ
り、老化に伴う新陳代謝の低下、特に皮膚のターンオー
バーの促進が図られ、若々しい肌を得ることができるこ
とが示唆される。On the other hand, 50% of the fluorescence intensity disappearance date was 3.66 days in the 2 groups to which the Tochu-Ginseng extract of the present invention and deoxyribonucleic acid were added, which is significantly faster than the 2 groups. It is suggested that metabolism is activated and the turnover period is shortened. Therefore, when the present invention is applied to humans, it promotes metabolism,
In particular, it is possible to promote the turnover of the skin, and it is suggested that the metabolism due to aging is lowered, especially the turnover of the skin is promoted, and youthful skin can be obtained.
【００２６】飼料の説明 上記検討で用いた６％蛋白質含有実験食及び１１％蛋白
質含有実験食の組成を表７に示す。 Description of feeds Table 7 shows the composition of the experimental diet containing 6% protein and the experimental diet containing 11% protein used in the above examination.
【表７】 ──────────────────────────────────── ６％実験食 １１％実験食 ──────────────────────────────────── ミルクカゼイン ７．０％ １３．０％ コーンスターチ ６３．０ ５７．０ グラニュー糖 １０．０ １０．０ コーンオイル ６．０ ６．０ アビセルセルロース ３．０ ３．０ ＫＣフロック ２．０ ２．０ オカノール（α化澱粉） １．０ １．０ 混合ビタミン １．０ １．０ 混合ミネラル ７．０ ７．０ ────────────────────────────────────[Table 7] ──────────────────────────────────── 6% Experimental food 11% Experimental food ── ────────────────────────────────── Milk Casein 7.0% 13.0% Corn Starch 63.0 57. 0 Granulated sugar 10.0 10.0 Corn oil 6.0 6.0 Avicel cellulose 3.0 3.0 KC floc 2.0 2.0 Ocanol (pregelatinized starch) 1.0 1.0 Mixed vitamin 1.0 1.0 Mixed minerals 7.0 7.0 ─────────────────────────────────────
【００２７】なお、上記表７の混合ビタミン及び混合ミ
ネラルはそれぞれ表７及び表８に記載の組成の混合物で
あり、単位はｍｇである。The mixed vitamins and mixed minerals in Table 7 above are mixtures having the compositions shown in Tables 7 and 8, respectively, and the unit is mg.
【表８】 ──────────────────────────────────── 混合ビタミン ──────────────────────────────────── ビタミンＡ・Ｄ3（５０万IU/１０万IU） ２．４ ｍｇ Ｅ（５０％） ２０．０ Ｋ3 ０．４ Ｂ1 １．５ Ｂ2 １．５６ Ｂ6 １．０２ Ｂ12（０．１％） ５．０ ビオチン（２．０％） ０．５ ＤＬ−パントテン酸Ｃａ ４．０ ＰＡＢＡ １０．１５ ニコチン酸 １０．１５ イノシトール １５．０ 葉酸 ０．２ 塩化コリン ３００．０ コーンスターチ ６２８．１２ ──────────────────────────────────── 合 計 １０００．０ ｍｇ ────────────────────────────────────[Table 8] ──────────────────────────────────── Mixed vitamins ──────── ──────────────────────────── Vitamin A ・ D 3 (500,000 IU / 100,000 IU) 2.4 mg E (50% ) 20.0 K 3 0.4 B 1 1.5 B 2 1.56 B 6 1.02 B 12 (0.1%) 5.0 biotin (2.0%) 0.5 DL- pantothenic acid Ca 4.0 PABA 10.15 Nicotinic acid 10.15 Inositol 15.0 Folic acid 0.2 Choline chloride 300.0 Corn starch 628.12 ────────────────────── ─────────────── Total 1000.0 mg ────────────────────────────── ───────
【００２８】[0028]
【表９】 ──────────────────────────────────── 混合ミネラル ──────────────────────────────────── ＣａＣＯ3 １３５５．４ ｍｇ ＫＨ3ＰＯ4 １７３０．０ ＣａＨＰＯ4・２Ｈ2Ｏ １５００．０ ＭｇＳＯ4・７Ｈ2Ｏ ８００．０ ＮａＣｌ ６００．０ ＦｅＣ6Ｃ5Ｏ7・５Ｈ2Ｏ １９０．０ ５ＺｎＯ・２ＣＯ2・４Ｈ2Ｏ ６．０ ＣｕＳＯ4・５Ｈ2Ｏ １．２６ ＣｏＣＬ2・６Ｈ2Ｏ ０．４ Ｃａ（ＩＯ3）2 １．５４ ＭｎＳＯ4・４Ｈ2Ｏ １５．４ コーンスターチ ８００．０ ──────────────────────────────────── 合 計 ７０００．０ ｍｇ ────────────────────────────────────[Table 9] ──────────────────────────────────── Mixed minerals ──────── ──────────────────────────── CaCO 3 1355.4 mg KH 3 PO 4 1730.0 CaHPO 4 · 2H 2 O 1500.0 MgSO 4 · 7H 2 O 800.0 NaCl 600.0 FeC 6 C 5 O 7 · 5H 2 O 190.0 5ZnO · 2CO 2 · 4H 2 O 6.0 CuSO 4 · 5H 2 O 1.26 CoCL 2 · 6H 2 O 0.4 Ca (IO 3 ) 2 1.54 MnSO 4 .4H 2 O 15.4 Cornstarch 800.0 ─────────────────────── ───────────── Total 7000.0 mg ──────────────────────────────── ────
【００２９】[0029]
【発明の効果】本発明の皮膚賦活剤及び皮膚賦活食品
は、新陳代謝を促進し、特に皮膚のターンオーバーを促
進することが可能であるという優れた効果を発揮するも
のである。INDUSTRIAL APPLICABILITY The skin activating agent and the skin activating food of the present invention exhibit an excellent effect of promoting metabolism, and particularly accelerating turnover of the skin.
フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ６１Ｋ 38/17 Ａ６１Ｋ 37/12 38/00 37/18 Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI technical display area A61K 38/17 A61K 37/12 38/00 37/18
Claims (2)
その抽出物とを必須成分とし、これにデオキシリボ核
酸、コンドロイチン硫酸、コラーゲン若しくはその加水
分解物、ハトムギエキスの一種又は二種以上を選択して
配合することを特徴とする皮膚賦活剤。1. Tochu or its extract and ginseng or its extract are essential components, and deoxyribonucleic acid, chondroitin sulfate, collagen or its hydrolyzate, and one or more kinds of coix extract are selected. A skin activating agent characterized by being blended.
ることを特徴とする皮膚賦活食品。2. A skin activating food, comprising the skin activating agent according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24863195A JP3308433B2 (en) | 1995-08-31 | 1995-08-31 | Skin activating food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24863195A JP3308433B2 (en) | 1995-08-31 | 1995-08-31 | Skin activating food |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0967262A true JPH0967262A (en) | 1997-03-11 |
| JP3308433B2 JP3308433B2 (en) | 2002-07-29 |
Family
ID=17180991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24863195A Expired - Lifetime JP3308433B2 (en) | 1995-08-31 | 1995-08-31 | Skin activating food |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3308433B2 (en) |
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|---|---|---|---|---|
| KR20010075842A (en) * | 2000-01-20 | 2001-08-11 | 김미혜 | Functional food |
| JP2002275078A (en) * | 2001-01-11 | 2002-09-25 | Kanebo Ltd | Lipolysis promoter |
| JP2004035456A (en) * | 2002-07-03 | 2004-02-05 | Pola Chem Ind Inc | Promoter for keratinocyte maturation and oral administration composition containing the same |
| JP2007039423A (en) * | 2004-12-24 | 2007-02-15 | Meiji Milk Prod Co Ltd | Fermented milk for skin amelioration and/or treatment and method for producing the same |
| JP2007063177A (en) * | 2005-08-31 | 2007-03-15 | Hayashibara Biochem Lab Inc | Composition for oral intake intended for lustrous skin |
| WO2008059927A1 (en) | 2006-11-15 | 2008-05-22 | Meiji Seika Kaisha, Ltd. | Collagen peptide composition and food or beverage containing the same |
| WO2008078387A1 (en) * | 2006-12-26 | 2008-07-03 | Meiji Dairies Corporation | Fermented milk for improving and/or treating skin and method for producing the same |
| JP2009149541A (en) * | 2007-12-19 | 2009-07-09 | Tottori Univ | Food or drink and medicine composition for reducing blood ammonia concentration |
| WO2010125910A1 (en) | 2009-04-28 | 2010-11-04 | 明治製菓株式会社 | Collagen peptide composition having good blood transfer properties, and food and drink containing same |
| JP2012188453A (en) * | 2004-12-24 | 2012-10-04 | Meiji Co Ltd | Skin improver and method for improving skin |
| JP2013116889A (en) * | 2011-10-31 | 2013-06-13 | Kowa Co | Boi containing composition |
| JP2014214141A (en) * | 2013-04-30 | 2014-11-17 | 興和株式会社 | Sinomenium stem-containing composition |
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| JPH06256139A (en) * | 1993-03-01 | 1994-09-13 | Kanebo Ltd | Beautifying cosmetic |
| JPH06336418A (en) * | 1993-05-28 | 1994-12-06 | Kose Corp | Cosmetic |
| JPH07278012A (en) * | 1994-04-11 | 1995-10-24 | Sunstar Inc | Metabolic promoter and food containing the same blended |
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| KR20010075842A (en) * | 2000-01-20 | 2001-08-11 | 김미혜 | Functional food |
| JP2002275078A (en) * | 2001-01-11 | 2002-09-25 | Kanebo Ltd | Lipolysis promoter |
| JP2004035456A (en) * | 2002-07-03 | 2004-02-05 | Pola Chem Ind Inc | Promoter for keratinocyte maturation and oral administration composition containing the same |
| JP2007039423A (en) * | 2004-12-24 | 2007-02-15 | Meiji Milk Prod Co Ltd | Fermented milk for skin amelioration and/or treatment and method for producing the same |
| JP2012188453A (en) * | 2004-12-24 | 2012-10-04 | Meiji Co Ltd | Skin improver and method for improving skin |
| JP2007063177A (en) * | 2005-08-31 | 2007-03-15 | Hayashibara Biochem Lab Inc | Composition for oral intake intended for lustrous skin |
| WO2008059927A1 (en) | 2006-11-15 | 2008-05-22 | Meiji Seika Kaisha, Ltd. | Collagen peptide composition and food or beverage containing the same |
| WO2008078387A1 (en) * | 2006-12-26 | 2008-07-03 | Meiji Dairies Corporation | Fermented milk for improving and/or treating skin and method for producing the same |
| JP2009149541A (en) * | 2007-12-19 | 2009-07-09 | Tottori Univ | Food or drink and medicine composition for reducing blood ammonia concentration |
| WO2010125910A1 (en) | 2009-04-28 | 2010-11-04 | 明治製菓株式会社 | Collagen peptide composition having good blood transfer properties, and food and drink containing same |
| JP2013116889A (en) * | 2011-10-31 | 2013-06-13 | Kowa Co | Boi containing composition |
| JP2014214141A (en) * | 2013-04-30 | 2014-11-17 | 興和株式会社 | Sinomenium stem-containing composition |
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