JPH0912454A - Theanine-containing composition - Google Patents
Theanine-containing compositionInfo
- Publication number
- JPH0912454A JPH0912454A JP7184923A JP18492395A JPH0912454A JP H0912454 A JPH0912454 A JP H0912454A JP 7184923 A JP7184923 A JP 7184923A JP 18492395 A JP18492395 A JP 18492395A JP H0912454 A JPH0912454 A JP H0912454A
- Authority
- JP
- Japan
- Prior art keywords
- theanine
- composition
- waves
- learning efficiency
- wave
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、テアニンを含有する組
成物がα波を出現、持続させる増強効果、また、学習効
率向上効果を持ち、その機能を食品、清涼飲料、乾燥
品、嗜好品および医薬品へ応用することを目的とする組
成物に関する。INDUSTRIAL APPLICABILITY The present invention provides a composition containing theanine, which has an enhancing effect of appearing and sustaining α-waves and an improving effect of learning efficiency, and its function is foods, soft drinks, dried products and favorite products. And to a composition intended for application in medicine.
【０００２】[0002]
【従来の技術】脳から出る微弱な電気を記録した脳波
は、周波数範囲によってδ波、θ波、α波、β波に分け
られる。その中でもα波は心が落ち着き、ゆったりした
気分の時に現れるため、リラックスの指標として挙げら
れる。α波はスロー、ミッド、ファストに分けられ、ス
ローは休息する方向に集中し、意識が低下して、ぼうっ
としている時、ミッドは緊張のないリラックスした状態
で集中しており、頭がさえている時、ファストは緊張し
た意識集中状態で、あまりゆとりのない時に出る脳波で
あるため、リラックス状態をはかるための有効な手段と
して注目されている。従来、このようなα波を出現させ
る物質としては、アプル香が知られている（G.E.Schwar
tz et al:Psycholology 16,281,1988 ）が、食品等への
応用においては、食品イメージと香りの相性、また、香
り自体の好き嫌いがあり、使用範囲が制限されてしまう
のが現状である。また、α波発生増強法としては胸式呼
吸に比べ、腹式呼吸では２倍以上のα波が発生するとい
う呼吸制御によるα波増強の可能性が認められている
（八木寛・作田利明：電子通信学会技術研究報告 Vol.
86,No.55,p1-8,1986）他、音楽や映像による方法も認め
られている。しかしながら、これらによってα波が現れ
る心の状態をつくりだすにはある種の訓練や機器、時
間、経費、場所が必要となり、容易にα波を増強させる
ことはできない。また、学習能力を向上させる成分とし
てドコサヘキサエン酸（ＤＨＡ）が広く用いられてお
り、またシアル酸を含有したスフィンゴ糖脂質の１つで
あるガングリオシドは学習能力の向上効果と深い関わり
があると推察されている（S.E.Karpiak,F.Vilim and Ma
hadik:Dev.Neurosci.,6,p127-135,1984)。しかしなが
ら、これらの学習能力を向上させる成分は独特の風味を
有するため、食品等への応用範囲が制限され、また天然
物に少量しか含まれていないため有効成分の分離・精製
が煩雑であり高コストであること等の問題点を有してい
る。2. Description of the Related Art An electroencephalogram, which records weak electricity emitted from the brain, is divided into δ wave, θ wave, α wave, and β wave depending on the frequency range. Among them, α waves appear when the mind is calm and relaxed, so they can be used as an index of relaxation. The alpha wave is divided into slow, mid, and fast, the slow is concentrated in the direction of rest, and when the consciousness is lowered and drowsy, the mid is concentrated in a relaxed state without tension, even the head is even When fasting, the fast is a tense state of concentration, and is an electroencephalogram that appears when there is not much space, so it is attracting attention as an effective means for achieving a relaxed state. Conventionally, an apple scent is known as a substance that causes such an α wave to appear (GESchwar
tz et al: Psycholology 16,281,1988) has limited the range of use in application to foods because of the compatibility between the food image and the scent, and the likes and dislikes of the scent itself. In addition, as a method for enhancing α-wave generation, it is possible to enhance α-waves by breathing control, in which more than twice the α-waves are generated in abdominal breathing compared to chest breathing (Yagi Yasu and Sakuta Toshiaki: IEICE Technical Report Vol.
86, No.55, p1-8, 1986) and other methods using music and images are also accepted. However, it requires some kind of training, equipment, time, cost, and place to create the state of mind in which α waves appear, and α waves cannot be easily enhanced. Docosahexaenoic acid (DHA) is widely used as a component for improving learning ability, and it is speculated that ganglioside, which is one of the glycosphingolipids containing sialic acid, is closely related to the effect of improving learning ability. (SEKarpiak, F.Vilim and Ma
hadik: Dev. Neurosci., 6, p127-135, 1984). However, since these ingredients that improve learning ability have unique flavors, the range of application to foods is limited, and since a small amount is contained in natural products, separation and purification of active ingredients is complicated and high. It has problems such as cost.
【０００３】また、従来テアニンは玉露の旨味成分とし
て知られ、茶をはじめとする食品の香味および調味成分
として重要でありその需要が高まりつつある。また一方
では、テアニンを含めたγ−グルタミル誘導体は、動・
植物体における生理活性物質として作用することが知ら
れている。例えば、テアニンはカフェインの中枢興奮作
用を抑制する物質であると考えられ（Chem.Pharm.Bul
l., 19(7) 1301-1307(1971),同19(6) 1257-1261(1971),
同34(7) 3053-3057(1986),薬学雑誌 95(7) 892-895(197
5), Agric.Biol.Chem., 51,3281-3286(1987), 同52, 31
73-3174(1988））、その生理活性物質としての有用性が
期待されている。さらに、テアニンの応用として、従
来、アセトアルデヒド毒性の抑制剤（特開平６−４０９
０１号）が開示されている。また、動物実験によるテア
ニンの抗ストレス剤（特開平６−１００４４２号）が開
示されている。本来、抗ストレス剤にはストレスの緩解
目的、抗てんかん作用、睡眠導入の目的にベンゾジアゼ
ピン系やチエノジアゼピン系の薬物が用いられている
が、多くの場合、副作用が危惧されるため、安全である
とは言い難い。このようにテアニンは、甘味に関係する
アミノ酸であると同時に、脳に関係する生理効果が調べ
られているが、脳波への影響については全く明らかでは
ない。現在までには音楽による脳波への影響や香りによ
る脳波への影響等、聴覚、嗅覚、視覚等の感覚の脳波へ
の影響は認められている。Further, theanine is conventionally known as an umami component of gyokuro, is important as a flavor and seasoning component of foods such as tea, and its demand is increasing. On the other hand, γ-glutamyl derivatives including theanine
It is known to act as a physiologically active substance in plants. For example, theanine is thought to be a substance that suppresses the central excitatory effect of caffeine (Chem.Pharm.Bul.
l., 19 (7) 1301-1307 (1971), ibid. 19 (6) 1257-1261 (1971),
Ibid 34 (7) 3053-3057 (1986), Pharmaceutical Journal 95 (7) 892-895 (197)
5), Agric.Biol.Chem., 51, 3281-3286 (1987), ibid 52, 31.
73-3174 (1988)), and its usefulness as a physiologically active substance is expected. Furthermore, as an application of theanine, a conventional inhibitor of acetaldehyde toxicity (Japanese Patent Laid-Open No. 6-409).
No. 01) is disclosed. Further, an anti-stress agent for theanine in animal experiments (JP-A-6-100442) is disclosed. Originally, benzodiazepines and thienodiazepines are used as antistress agents for the purpose of relieving stress, antiepileptic action, and sleep induction, but in many cases, side effects are feared, so it is safe. Hard to say. As described above, theanine is an amino acid related to sweetness, and at the same time, a physiological effect related to the brain has been investigated, but its effect on the electroencephalogram is not clear at all. Up to now, the influence of the senses such as hearing, smell and sight on the brain waves such as the influence of music on the brain waves and the influence of fragrance on the brain waves have been recognized.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、簡単な摂取
によって容易に精神的リラックスと深く係わっているα
波を発生させ、持続させ、学習効率を向上させる物質を
提供するものである。DISCLOSURE OF THE INVENTION The present invention is easily associated with mental relaxation by simple intake.
It provides substances that generate and sustain waves and improve learning efficiency.
【０００５】[0005]
【課題を解決するための手段】本発明者らは、このよう
なα波出現および持続に効果のある、また、学習効率向
上に効果のある物質の検討を行ったところ、緑茶に多く
含まれているアミノ酸の一種、テアニンがこのような効
果を持つことを見いだし、本発明を完成した。テアニン
のα波出現増強効果、学習効率向上効果についてはこれ
まで知られておらず、本発明者らが初めて見いだした新
規効果である。以下、本発明について詳述する。[Means for Solving the Problems] The present inventors have examined substances that are effective in the appearance and maintenance of such α-waves and in improving learning efficiency. The present invention has been completed by discovering that theanine, a type of amino acid, has such an effect. The effect of enhancing the appearance of α-waves and the effect of improving learning efficiency of theanine have not been known so far, and they are novel effects discovered by the present inventors for the first time. Hereinafter, the present invention will be described in detail.
【０００６】本発明に用いられるテアニンは、茶の葉に
含まれているグルタミン酸誘導体で、茶の旨味の主成分
である。その含有量は、他のアミノ酸よりも高く、乾燥
茶葉１００ｇ中に玉露（上）では２４６６．１mg、玉露
（並）では２００７．７mg、煎茶（上）では１４９６．
６mg、煎茶（並）では６５２．５mg、番茶では４１６．
７mg、ほうじ茶では２１．７mgと報告されている（茶研
報 No.40, 65, 1973）。また、呈味を用途とする食品添
加物として使用されており、その添加量には制限はな
い。本発明に用いられるテアニンの製造法としては、有
機合成法（Chem.Pharm.Bull., 19(7) 1301-1307(197
1))、発酵法（特開平５−６８５７８号、特開平５−３
２８９８６号）、植物細胞培養法（特開平５−１２３１
６６）等があり、いずれの方法でも良い。また、このよ
うな方法により得られたテアニンはＬ−体、Ｄ−体、Ｄ
Ｌ−体いずれも使用可能であるが、中でもＬ−体は天然
物由来であるため、含まれる不純物も食品として摂取で
き、高度精製の必要がないため、本発明においてはＬ−
体が好ましい。Theanine used in the present invention is a glutamic acid derivative contained in tea leaves, which is the main component of the umami of tea. Its content is higher than other amino acids, and 2466.1 mg of gyokuro (above), 2007.7 mg of gyokuro (normal) and 1496. of sencha (above) in 100 g of dried tea leaves.
6mg, Sencha (normal) 652.5mg, Bancha 416.
It is reported to be 7 mg and 21.7 mg for Hojicha (Tea Kenkyu No. 40, 65, 1973). It is also used as a food additive for taste, and the amount added is not limited. The method for producing theanine used in the present invention includes an organic synthesis method (Chem. Pharm. Bull., 19 (7) 1301-1307 (197).
1)), fermentation method (JP-A-5-68578, JP-A-5-3)
28986), a plant cell culture method (JP-A-5-1231).
66) and the like, and any method may be used. In addition, theanine obtained by such a method is L-form, D-form, D-form.
Any of the L-forms can be used, but among them, since the L-form is derived from a natural product, the impurities contained therein can be ingested as foods, and there is no need for advanced purification.
The body is preferred.
【０００７】本発明におけるα波出現増強とは、被験者
に電極を装着し脳波計を用いて脳波を測定した時、α波
の出現時間の累計が平常時に比べ、１０％以上増加し、
且つ、摂取後６０分まで１０分毎のα波の出現時間が減
少せず、持続するものである。測定例としては、例えば
被験者は外部から遮断された閉鎖環境室にて椅子座位で
脳波計 NEC SYNAFIT １０００を用いて単極導出法で測
定する方法があげられるが、本発明の測定方法の範囲は
これに限定されるものではなく、国際標準法の単極導出
法や双極導出法等脳波測定に関する公知の方法であれば
よい。また、学習効率向上には、脳の高度な働きが必要
であるが、人間が暗算など作業に集中している時にはα
波が出ていることが報告されており（河野貴美子他：FR
AGRANCE JOURNAL 研究報告，p111-118，1994-2）、緊張
を伴わない集中状態の形成や自律機能の安定化、また、
こだわりにとらわれない素直な見方・考え方を可能に
し、潜在能力発揮の鋭敏化、精神の感覚の鋭敏化をする
ことにより、集中力や思考力、判断力、記憶力をあげる
ことである。The α wave appearance enhancement in the present invention means that when the subject is equipped with electrodes and the EEG is measured using an electroencephalograph, the cumulative α wave appearance time is increased by 10% or more as compared with normal times.
Moreover, the appearance time of the α wave every 10 minutes does not decrease and continues until 60 minutes after ingestion. As an example of the measurement, for example, the test subject can be measured by the unipolar derivation method using the electroencephalograph NEC SYNAFIT 1000 in a sitting position in a closed environment room that is shielded from the outside. The method is not limited to this, and any known method relating to brain wave measurement such as the international standard method of unipolar derivation or bipolar derivation may be used. Also, to improve learning efficiency, advanced brain functions are required, but when humans concentrate on tasks such as mental arithmetic, α
It is reported that waves are coming (Kumiko Kono et al .: FR
AGRANCE JOURNAL research report, p111-118, 1994-2), formation of concentration state without tension and stabilization of autonomic function,
It is to increase concentration, thinking, judgment, and memory by enabling an unobtrusive, straightforward view and way of thinking, and by sensitizing potential performance and mental sensation.
【０００８】本発明のα波出現増強組成物の有効成分で
あるテアニンは、そのまま使用してもよいが、食品、乾
燥品、嗜好品に添加する製剤、清涼飲料やミネラルウォ
ーター、嗜好飲料、アルコール飲料、ドリンク剤に添加
する可溶性製剤としても使用可能である。また、医薬品
としてもカプセル剤、錠剤、粉末剤、顆粒剤、ドリンク
剤、注射剤、点滴剤等に製剤することができる。Ｌ−テ
アニンはマウスを用いた急性毒性試験において２ｇ／kg
経口投与で死亡例はなく、一般症状及び体重等に異常は
認められず、非常に弱毒または無害の物質であるため、
保健的効果のある食品素材および飲料素材としての発展
が望める。また、他の成分（精油、アミノ酸、ビタミン
等）とも併せて使用できる。Theanine, which is the active ingredient of the α-wave appearance enhancing composition of the present invention, may be used as it is, but it may be used as it is, a food, a dry product, a preparation to be added to a favorite product, a soft drink, mineral water, a favorite drink, or an alcohol. It can also be used as a soluble preparation to be added to beverages and drinks. In addition, it can be formulated as a medicine into capsules, tablets, powders, granules, drinks, injections, infusions and the like. L-theanine is 2g / kg in an acute toxicity test using mice.
There are no deaths after oral administration, no abnormalities in general symptoms and body weight, etc. are observed, and since it is a very attenuated or harmless substance,
It can be expected to develop as a food material and a beverage material with health effects. It can also be used in combination with other components (essential oils, amino acids, vitamins, etc.).
【０００９】本発明のテアニン含有組成物をα波出現増
強組成物として用いるには、テアニンとして体重１kg当
たり０．３mg（以下０．３mg／kgの如く表示する）以上
を投与すれば充分な効果を得ることができる。また、学
習効率向上組成物として用いるには、０．３mg／kg以上
を投与すれば充分な効果を得ることができるが、投与量
を増加させると効果はより一層増強する。テアニンの投
与量に特に上限は存在しないが、テアニンの特有の呈味
と経済性を考慮すると一般的に３００mg／kg程度を越え
ないことが好ましい。従って、本発明のテアニン含有組
成物がその効果を充分に発揮するためには、テアニンを
１回服用当たり０．３mg／kg〜３００mg／kg含有するこ
とが好ましい。さらに望ましくは０．３mg／kg〜３０mg
／kg含有することが好ましい。また、０．３〜３mg／kg
とすることは最も好ましい。次に実施例によって本発明
をさらに説明するが、本発明の範囲はこれらのみに限定
されるものではない。In order to use the theanine-containing composition of the present invention as an α-wave appearance enhancing composition, it is sufficient to administer 0.3 mg or more of theanine per kg of body weight (hereinafter expressed as 0.3 mg / kg). Can be obtained. Further, for use as a learning efficiency improving composition, a sufficient effect can be obtained by administering 0.3 mg / kg or more, but the effect is further enhanced by increasing the dose. Although there is no particular upper limit to the dose of theanine, it is generally preferred that the dose does not exceed about 300 mg / kg in view of the unique taste and economics of theanine. Therefore, in order for the theanine-containing composition of the present invention to sufficiently exert its effects, it is preferable to contain theanine in an amount of 0.3 mg / kg to 300 mg / kg per single dose. More desirably 0.3 mg / kg to 30 mg
/ Kg is preferable. In addition, 0.3 to 3 mg / kg
Is most preferable. Next, the present invention will be further described with reference to examples, but the scope of the present invention is not limited to these.
【００１０】[0010]
実施例１ ０．３Ｍグルタミン及び１．５Ｍエチルアミンをホウ酸
緩衝液（Na2B4O7 −NaOH、pH11）中、０．３Ｕ／mlグル
タミナーゼにて３０℃、２２時間反応させた。反応液１
より２２５mmolのテアニンを得た。なお、副生成物のグ
ルタミン酸は２０mmolであった。なお、反応液からの精
製は、反応液をDowex 50×８、Dowex １×２カラムクロ
マトグラフィーにかけ、これをエタノール処理すること
により行った。テアニンの確認はこの単離物質をアミノ
酸アナライザー、ペーパークロマトグラフィーにかける
と、標準物質と同じ挙動を示すことにより行った。塩酸
またはグルタミナーゼで加水分解処理を行なうと、１：
１の割合で、グルタミン酸とエチルアミンを生じた。こ
のように、単離物質がグルタミナーゼによって加水分解
されたことから、エチルアミンがグルタミン酸のγ位に
結合していたことが示される。また、加水分解で生じた
グルタミン酸がＬ型であることも、グルタミン酸デヒド
ロゲナーゼ（GluDH)により確認され、得られた化合物が
Ｌ−テアニンであることを確認した。Example 1 0.3 M glutamine and 1.5 M ethylamine were reacted in a borate buffer (Na 2 B 4 O 7 -NaOH, pH 11) with 0.3 U / ml glutaminase at 30 ° C. for 22 hours. Reaction liquid 1
225 mmol of theanine was obtained. In addition, glutamic acid as a by-product was 20 mmol. The purification from the reaction solution was performed by subjecting the reaction solution to Dowex 50 × 8 and Dowex 1 × 2 column chromatography, and treating this with ethanol. Theanine was confirmed by subjecting this isolated substance to an amino acid analyzer and paper chromatography to show the same behavior as the standard substance. When hydrolyzed with hydrochloric acid or glutaminase, 1:
Glutamic acid and ethylamine were produced in one ratio. Thus, the isolated substance was hydrolyzed by glutaminase, indicating that ethylamine was bound to the γ-position of glutamic acid. It was also confirmed by glutamate dehydrogenase (GluDH) that the glutamic acid produced by hydrolysis was L-type, and it was confirmed that the obtained compound was L-theanine.
【００１１】実施例２ 茶（Camellia sinensis L.）葉を熱水で抽出後、カチオ
ン交換樹脂（室町化学工業（株）製 Dowex HCR W-２）
に通し、１Ｎ NaOHにより溶出した。溶出画分を活性炭
（二村化学工業（株）製 太閤活性炭 SG）に通し、１
５％EtOHによる溶出画分をRO膜（日東電工（株）製 NT
R 729 HF）を用いて濃縮し、カラムクロマトグラフィー
にて精製し、更に再結晶を行い、テアニンを製造した。 試験例１ 実施例１で得られた本発明品であるテアニン含有組成物
のα波の出現と持続効果を調べるため、人にテアニン含
有組成物を摂取させた。試験には実施例１で得られたテ
アニン含有組成物２００mgをミネラルウォーター１００
mlに溶解したテアニン含有組成物水（以下テアニン含有
組成物水と称す）を供した（約３mg／kgに相当する）。
まず、被験者は外部から遮断された２５℃、４０ルクス
の閉鎖環境室にて椅子座位で脳波を測定した。脳波測定
には脳波計 NEC SYNAFIT １０００を用いた。測定は被
験者６名に対して行い、被験者の両耳たぶ（電位零と考
えられる点）に基準電極を置き、頭部１２ヶ所に探査電
極（脳波をとらえるために頭の表面に置かれた電極）を
装着し、単極導出法で行った。測定時間はテアニン含有
組成物水または水を１００ml摂飲後１時間とした。Example 2 Tea (Camellia sinensis L.) leaves were extracted with hot water and then cation exchange resin (Muromachi Chemical Co., Ltd. Dowex HCR W-2)
And eluted with 1N NaOH. Pass the eluted fraction through activated carbon (Taiko Activated Carbon SG, manufactured by Nimura Chemical Industry Co., Ltd.)
The fraction eluted with 5% EtOH was subjected to RO membrane (NTTO Denko Corporation NT
R 729 HF) was used for concentration, purification was performed by column chromatography, and recrystallization was performed to produce theanine. Test Example 1 In order to investigate the appearance and sustained effect of α-wave of the theanine-containing composition of the present invention obtained in Example 1, a human was allowed to ingest the theanine-containing composition. For the test, 200 mg of the theanine-containing composition obtained in Example 1 was added to 100 parts of mineral water.
The theanine-containing composition water dissolved in ml (hereinafter referred to as theanine-containing composition water) was provided (corresponding to about 3 mg / kg).
First, the test subject measured an electroencephalogram in a sitting position in a closed environment room of 40 lux at 25 ° C., which was shielded from the outside. An electroencephalograph NEC SYNAFIT 1000 was used for EEG measurement. The measurement was performed on 6 subjects, and reference electrodes were placed on the lobes of both subjects (points at which the potential is zero), and probe electrodes were placed at 12 positions on the head (electrodes placed on the surface of the head to capture brain waves). It was attached by using the monopole derivation method. The measurement time was 1 hour after drinking 100 ml of the theanine-containing composition water or water.
【００１２】水、テアニン含有組成物水摂飲後のα波を
脳波形より読み取り、１０分毎のα波の出現時間と出現
回数を算出した。結果を図１、図２に示す。図１、図２
より明らかなようにテアニン含有組成物水を摂飲した時
の方が対照の水を摂飲した時よりα波の出現時間が増加
しており、摂取後４０分以降も水摂飲ではα波の出現時
間が減少するのに対して、テアニン含有組成物水摂飲で
は持続効果を発揮し、α波の出現を増強させる効果を発
揮した。 試験例２ 実施例１および実施例２で得られた本発明品であるテア
ニン含有組成物を６０mg、３００mg、１２００mgミネラ
ルウォーター１００mlに溶解したテアニン含有組成物水
（それぞれ約１mg／kg、５mg／kg、２００mg／kgに相当
する）を男女合計７名にそれぞれ摂飲させ、呈味の有無
について官能試験を行った。結果を表１に示す。Composition containing water and theanine The α-wave after drinking water was read from the brain waveform, and the appearance time and the number of appearances of the α-wave every 10 minutes were calculated. The results are shown in FIGS. 1 and 2
As is clearer, when the water containing the theanine-containing composition was ingested, the appearance time of the α-wave was longer than that when the control water was ingested, and the α-wave was observed even after 40 minutes after ingestion. In contrast to the decrease in the appearance time of the, the water-ingested theanine-containing composition exerted a long-lasting effect and an effect of enhancing the appearance of α-waves. Test Example 2 Theanine-containing composition water obtained by dissolving the theanine-containing composition of the present invention obtained in Examples 1 and 2 in 100 ml of 60 mg, 300 mg, 1200 mg mineral water (about 1 mg / kg and 5 mg / kg, respectively) , Which is equivalent to 200 mg / kg) was ingested by a total of 7 men and women, and a sensory test was conducted for the presence or absence of taste. Table 1 shows the results.
【００１３】[0013]
【表１】 [Table 1]
【００１４】表１より明らかなようにテアニン含有組成
物を３００mg以上含有すると呈味を感じる人がいるが、
６０mgでは感じず、風味には影響が生じなかった。この
ことより呈味を感じない上限を調べた結果、２００mgま
では風味に影響が生じなかった。 試験例３ 実施例１および実施例２で得られた本発明品であるテア
ニン含有組成物の学習効率向上効果を調べるため、ラッ
トを用いて実験を行った。まず、５週令のラットにサン
プルを飼料中に規定量添加した。ラットへのサンプルの
投与は、１週間行った。試験区として本発明品であるテ
アニン含有組成物１mg／kg、１０mg／kg、２００mg／kg
（人間の代謝に換算すると、それぞれ約 0.3mg／kg、2.
5mg ／kg、５０mg／kgに相当する）を含有するテアニン
含有組成物水、生理食塩水で実験を行った。投与後から
１週間、毎日図３に示す迷路を走らせ、スタートからゴ
ールまでの到達時間の平均から各サンプルの学習効率向
上効果を測定した。結果を図４に示す。図４より明らか
なようにテアニン含有組成物は生理食塩水以上にゴール
までの到達時間は短縮されており、学習効率の向上に優
れた効果を発揮した。As is clear from Table 1, there are some people who feel a taste when the composition containing 300 mg or more of theanine is contained.
At 60 mg, it was not felt and the flavor was not affected. As a result, the upper limit at which the taste was not felt was examined. As a result, the flavor was not affected up to 200 mg. Test Example 3 In order to examine the learning efficiency improving effect of the theanine-containing composition of the present invention obtained in Example 1 and Example 2, an experiment was conducted using rats. First, a predetermined amount of a sample was added to the diet of a 5-week-old rat. The samples were administered to the rats for 1 week. 1 mg / kg, 10 mg / kg, 200 mg / kg of theanine-containing composition of the present invention as a test section
(Converted to human metabolism, about 0.3 mg / kg, 2.
Experiments were carried out with the theanine-containing composition water containing 5 mg / kg, corresponding to 50 mg / kg) and physiological saline. The maze shown in FIG. 3 was run daily for 1 week after the administration, and the learning efficiency improving effect of each sample was measured from the average of the arrival times from the start to the goal. FIG. 4 shows the results. As is clear from FIG. 4, the theanine-containing composition had a shorter time to reach the goal than the physiological saline, and exhibited an excellent effect in improving learning efficiency.
【００１５】本発明の実施態様をあげれば以下の通りで
ある。 （１）テアニンを含有することを特徴とするα波出現増
強組成物。 （２）テアニンがＬ−テアニンであるα波出現増強組成
物。 （３）テアニンがＤ−テアニンであるα波出現増強組成
物。 （４）テアニンがＤＬ−テアニンであるα波出現増強組
成物。 （５）テアニンをグルタミンとエチルアミンの混合物に
グルタミナーゼを作用させることにより製造することを
特徴とする前記（１）〜（４）記載のα波出現増強組成
物。 （６）テアニンが茶由来であることを特徴とする前記
（１）〜（２）記載のα波出現増強組成物。 （７）テアニンを含有することを特徴とする学習効率向
上組成物。 （８）テアニンがＬ−テアニンである学習効率向上組成
物。 （９）テアニンがＤ−テアニンである学習効率向上組成
物。 （１０）テアニンがＤＬ−テアニンである学習効率向上
組成物。 （１１）テアニンをグルタミンとエチルアミンの混合物
にグルタミナーゼを作用させることにより製造すること
を特徴とする前記（７）〜（１０）記載の学習効率向上
組成物。 （１２）Ｌ−テアニンが茶由来であることを特徴とする
前記（７）〜（８）記載の学習効率向上組成物。The embodiments of the present invention are as follows. (1) An α-wave appearance enhancing composition comprising theanine. (2) An α-wave appearance enhancing composition in which theanine is L-theanine. (3) An α-wave appearance enhancing composition in which theanine is D-theanine. (4) An α-wave appearance enhancing composition in which theanine is DL-theanine. (5) The α-wave appearance enhancing composition as described in (1) to (4) above, wherein theanine is produced by allowing glutaminase to act on a mixture of glutamine and ethylamine. (6) The α-wave appearance enhancing composition according to the above (1) or (2), wherein theanine is derived from tea. (7) A composition for improving learning efficiency, which comprises theanine. (8) A learning efficiency improving composition in which theanine is L-theanine. (9) A learning efficiency improving composition in which theanine is D-theanine. (10) A learning efficiency improving composition in which theanine is DL-theanine. (11) The learning efficiency improving composition as described in (7) to (10) above, wherein theanine is produced by allowing glutaminase to act on a mixture of glutamine and ethylamine. (12) The learning efficiency improving composition as described in (7) to (8) above, wherein L-theanine is derived from tea.
【００１６】[0016]
【発明の効果】以上のように本発明品であるテアニン含
有組成物は容易にα波の出現と持続を増強することがで
き、また、学習効率を向上させることができる。本発明
品を用いることは味、香り等において食品、医薬品等に
応用範囲が広く、効果、安全性の点をも考え併せて極め
て有益である。Industrial Applicability As described above, the theanine-containing composition of the present invention can easily enhance the appearance and duration of α-waves and improve the learning efficiency. The use of the product of the present invention has a wide range of applications in foods, pharmaceuticals, etc. in terms of taste, fragrance, etc., and is extremely useful in consideration of effects and safety.
【００１７】[0017]
【図１】α波の出現回数に及ぼすテアニンの効果を示
し、左脳と右脳のα波の合計値を示した図である。FIG. 1 is a diagram showing the effect of theanine on the number of appearances of α-waves and showing the total value of α-waves of the left and right brains.
【図２】α波の出現時間に及ぼすテアニンの効果を示
し、左脳と右脳のα波の合計値を示した図である。FIG. 2 is a diagram showing the effect of theanine on the appearance time of α-waves and showing the total value of α-waves of the left and right brains.
【図３】迷路の平面図である。FIG. 3 is a plan view of a maze.
【図４】ゴールまでの到達時間の図である。FIG. 4 is a diagram of arrival time to a goal.
Claims (2)
波出現増強組成物。1. An α containing theanine
Wave appearance enhancing composition.
習効率向上組成物。2. A composition for improving learning efficiency, which comprises theanine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18492395A JP4627813B2 (en) | 1995-06-27 | 1995-06-27 | Theanine-containing composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18492395A JP4627813B2 (en) | 1995-06-27 | 1995-06-27 | Theanine-containing composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0912454A true JPH0912454A (en) | 1997-01-14 |
| JP4627813B2 JP4627813B2 (en) | 2011-02-09 |
Family
ID=16161703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18492395A Expired - Lifetime JP4627813B2 (en) | 1995-06-27 | 1995-06-27 | Theanine-containing composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4627813B2 (en) |
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|---|---|---|---|---|
| KR19990004304A (en) * | 1997-06-27 | 1999-01-15 | 손경식 | Alpha-wave enhanced beverage composition |
| KR100235110B1 (en) * | 1997-10-29 | 1999-12-15 | 서경배 | Tea composition containing green tea leaves and green tea leaves with a high GABA content |
| JP2000247878A (en) * | 1999-02-23 | 2000-09-12 | Taiyo Kagaku Co Ltd | Theanine-containing composition |
| JP2001031566A (en) * | 1999-07-19 | 2001-02-06 | Taiyo Kagaku Co Ltd | Inhibitory composition for problematic behavior of pet |
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Cited By (26)
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| KR19990004304A (en) * | 1997-06-27 | 1999-01-15 | 손경식 | Alpha-wave enhanced beverage composition |
| KR100235110B1 (en) * | 1997-10-29 | 1999-12-15 | 서경배 | Tea composition containing green tea leaves and green tea leaves with a high GABA content |
| JP2001089365A (en) * | 1998-02-23 | 2001-04-03 | Taiyo Kagaku Co Ltd | Anxiety inhibitory composition |
| EP1057483A4 (en) * | 1998-02-23 | 2002-06-26 | Taiyo Kagaku Kk | Composition comprising theanine |
| US6589566B2 (en) | 1998-02-23 | 2003-07-08 | Tomoko Ueda | Composition comprising theanine |
| JP2000247878A (en) * | 1999-02-23 | 2000-09-12 | Taiyo Kagaku Co Ltd | Theanine-containing composition |
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| JP4961087B2 (en) * | 2000-04-05 | 2012-06-27 | 太陽化学株式会社 | Sleep promoting composition |
| US8293269B2 (en) | 2000-04-05 | 2012-10-23 | Taiyo Kagaku Co., Ltd. | Compositions for promoting sleep |
| JP2001316256A (en) * | 2000-04-28 | 2001-11-13 | Taiyo Kagaku Co Ltd | Composition for ameliorating blood flow |
| US7094787B2 (en) | 2000-07-21 | 2006-08-22 | Taiyo Kagaku Co., Ltd. | Compositions for regulating desire for smoking |
| WO2002007723A1 (en) * | 2000-07-21 | 2002-01-31 | Taiyo Kagaku Co., Ltd. | Compositions for regulating desire for smoking |
| US9724318B2 (en) | 2001-04-24 | 2017-08-08 | Taiyo Kagaku Co., Ltd. | Compositions for improving mental concentration |
| WO2002089786A1 (en) * | 2001-04-24 | 2002-11-14 | Taiyo Kagaku Co., Ltd. | Compositions for improving mental concentration |
| JP2002322053A (en) * | 2001-04-24 | 2002-11-08 | Taiyo Kagaku Co Ltd | Composition for improving mental concentration |
| CN100341499C (en) * | 2001-04-24 | 2007-10-10 | 太阳化学株式会社 | Compsns. for improving mental concentration |
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| JP2002363074A (en) * | 2001-06-06 | 2002-12-18 | Taiyo Kagaku Co Ltd | Composition for improving attention-deficit hyperactivity disorder |
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| JP2003055212A (en) * | 2001-08-09 | 2003-02-26 | Hidehiko Yokogoshi | Dysmenorrhea inhibitory composition |
| CN1297217C (en) * | 2002-04-19 | 2007-01-31 | 三井制糖株式会社 | Alpha wave emitting enhancing agent, agent capable of leading relaxation state and diet contg. above-mentioned agents |
| JP4695412B2 (en) * | 2005-03-08 | 2011-06-08 | 太陽化学株式会社 | Bath additive |
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