JPH0889237A - New strain of bacillus thuringiensis - Google Patents

New strain of bacillus thuringiensis

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Publication number
JPH0889237A
JPH0889237A JP6261515A JP26151594A JPH0889237A JP H0889237 A JPH0889237 A JP H0889237A JP 6261515 A JP6261515 A JP 6261515A JP 26151594 A JP26151594 A JP 26151594A JP H0889237 A JPH0889237 A JP H0889237A
Authority
JP
Japan
Prior art keywords
bacillus thuringiensis
new strain
active ingredient
insecticide
isolate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6261515A
Other languages
Japanese (ja)
Other versions
JP3251137B2 (en
Inventor
Kazuhiko Higuchi
和彦 樋口
Hiroyuki Saito
浩之 斉藤
Hidekazu Mizushiro
英一 水城
Michio Oba
道夫 大庭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FUKUOKA PREF GOV
Fukuoka Prefecture
Original Assignee
FUKUOKA PREF GOV
Fukuoka Prefecture
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Priority to JP26151594A priority Critical patent/JP3251137B2/en
Publication of JPH0889237A publication Critical patent/JPH0889237A/en
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Publication of JP3251137B2 publication Critical patent/JP3251137B2/en
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Abstract

PURPOSE: To obtain an insecticide for Telmatoscopus albipunctatus, containing a cultured product of a separated new strain of Bacillus thuringiensis as an active ingredient and useful in exterminating the Telmatoscopus albipunctatus. CONSTITUTION: This new strain 88-KO-14-45 (FERM P-14496) of Bacillus thuringiensis has specific insecticidal activities against Telmatoscopus albipunctatus. Furthermore, an insecticide for the Telmatoscopus albipunctatus contains a cultured product of the separated new strain 88-KO-14-45 (FERM P-14496) of the Bacillus thuringiensis as an active ingredient at a concentration of about 1×10<8> to 10<10> spores/ml expressed in terms of spore concentration. The spore which is the active ingredient or a crystalline toxic protein produced by the spare is capable of manifesting specific strong insecticidal activities against larvae of the Telmatoscopus albipunctatus.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は、双翅目害虫、特にオオ
チョウバエに対して殺虫活性のある新規なバチルス・チ
ューリンゲンシス(Bacillus thuringiensis ) 分離株に
関する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel Bacillus thuringiensis isolate having an insecticidal activity against Diptera pests, especially Drosophila melanogaster.

【0002】[0002]

【従来の技術】化学殺虫剤に代わる微生物殺虫剤とし
て、最も広く利用されている種々のバチルス・チューリ
ンゲンシス(Bacillus thuringiensis ・ BT) は、胞子形
成期に結晶性毒素タンパクを形成し、この毒素タンパク
を摂食した害虫は、腸管破裂等ののち死に至ることが知
られている。
2. Description of the Related Art Bacillus thuringiensis (BT), which is the most widely used microbial insecticide as an alternative to chemical insecticides, forms a crystalline toxin protein during the sporulation period. It is known that the pests that feed on peaches die after rupture of the intestinal tract and the like.

【0003】BTはその亜種の違いにより殺虫活性が異
なっており、例えば亜種クルスタキー、アイザワイは、
鱗翅目、双翅目昆虫に毒性を示し(Dulmage,H.T.: J. In
vertebr. Pathol., 15, 232 (1970)) 、イスラエレンシ
スは双翅目昆虫に(L. J. Goldberg : Mosq. News, 3
7, 355 (1977)) 、またテネブリオニスは鞘翅目の甲虫
類にそれぞれ毒性を示すことが知られている(特開昭6
0ー156382)。しかし微生物殺虫剤が化学殺虫剤
に比べその対象とする害虫の適用範囲が狭いため、その
適用範囲を広げる目的で、数種のBTを混合して使用し
たり、遺伝子工学的手法により適用範囲を広げるなどの
方法が取られている。
The insecticidal activity of BT differs depending on the subspecies thereof. For example, the subspecies Kurstaky and Aizawai are:
Toxic to Lepidoptera and Diptera insects (Dulmage, HT: J. In
vertebr. Pathol., 15, 232 (1970)), Isla Ellensis turns into a diptera (LJ Goldberg: Mosq. News, 3
7, 355 (1977)), and Tenebrionis is known to be toxic to Coleoptera beetles, respectively (Japanese Patent Laid-open No. Sho 6-62).
0-156382). However, microbial pesticides have a narrower range of application of target pests than chemical pesticides. Therefore, in order to expand the range of application, several types of BT can be mixed and used, or the range of application can be changed by genetic engineering techniques. Ways such as unfolding are taken.

【0004】しかし一方では、適用範囲の広いBT殺虫
剤を使用したり、数種のBT殺虫剤を使用することは、
化学農薬と同様の弊害を導くこととなる。つまりその広
いホストレンジのために有益な昆虫や寄生生物に対して
も活性を示し、自然環境に備わっているバイオロジカル
コントロール作用をも破壊してしまう危険がある。特
に、家庭用の簡易浄化槽や、下水処理槽に大量に発生す
る不快衛生害虫はその生育環境条件によりほぼ均一の種
からなり、これを駆除するためには狭いスペクトルのB
T殺虫剤を用いた方が、環境に対する安全性を考慮した
場合適している。例えば、汚水ろ過槽に発生するハエ類
の駆除にイスラエレンシスを使用した報告があるが(J.
Houston : Wat. Res. 23, 379, (1989))、その適用害虫
はLimnophyes minimus , Metoriocnemus hygropetricu
s, Psychoda severini, Psychoda alternata, Sylvico
la fenestralis, Orthocladius fuscimanusと広く、双
翅目昆虫の多くに殺虫活性を示す。この報告を含め家庭
用の簡易浄化槽や、下水処理槽等で特異的に発生する不
快衛生害虫のオオチョウバエ(Telmatoscopus albipunct
atus) に特異的な殺虫活性を示すBT殺虫剤の報告はい
まのところ皆無である。
On the other hand, however, the use of BT insecticides with a wide range of application and the use of several BT insecticides
It will lead to the same adverse effects as chemical pesticides. In other words, because of its wide host range, it exhibits activity against beneficial insects and parasites, and there is a risk of destroying the biological control action that is provided in the natural environment. In particular, unpleasant hygienic pests that occur in large quantities in household simple septic tanks and sewage treatment tanks consist of almost uniform seeds depending on their growth environment conditions.
The use of T insecticide is more suitable when considering the safety to the environment. For example, there are reports of using Isla Ellensis to control flies that occur in sewage filtration tanks (J.
Houston: Wat. Res. 23, 379, (1989)), applicable pests are Limnophyes minimus, Metoriocnemus hygropetricu
s, Psychoda severini, Psychoda alternata, Sylvico
La fenestralis and Orthocladius fuscimanus are widespread and show insecticidal activity to many Diptera insects. Including this report, the unfair sanitary insect pest, Drosophila melanogaster (Telmatoscopus albipunct), which occurs specifically in simple household septic tanks and sewage treatment tanks, etc.
At this time, there are no reports of BT insecticides showing a specific insecticidal activity against (atus).

【0005】[0005]

【発明が解決しようとする課題】本発明の目的は、上記
の問題を解決するためにオオチョウバエ(Telmatoscopus
albipunctatus) に特異的に殺虫活性を示す新規なバチ
ルス・チューリンゲンシス(Bacillus thuringiensis)
分離株を得ることにあり、さらに、この分離株を用いる
オオチョウバエの駆除に有効な殺虫剤ないしは殺虫法を
提供することにある。
SUMMARY OF THE INVENTION It is an object of the present invention to solve the above-mentioned problems.
albipunctatus), a novel Bacillus thuringiensis with insecticidal activity
The purpose of the present invention is to obtain an isolated strain, and further to provide an insecticide or an insecticidal method effective for controlling Drosophila melanogaster using this isolated strain.

【0006】[0006]

【課題を解決するための手段】本発明者は、双翅目害虫
のなかでも特にオオチョウバエに対して殺虫活性を持っ
たバチルス・チューリンゲンシス(Bacillus thuringien
sis)新菌株の分離に成功することにより、この課題を解
決した。本発明者が88−KO−14−45と表示する
この新規分離菌株は、工業技術院生命工学工業技術研究
所に、受託番号FERM P−14496として寄託さ
れており、次に示すような特性を有している。
Means for Solving the Problems Among the dipteran pests, the present inventor has found that Bacillus thuringiensis has an insecticidal activity against Drosophila melanogaster, in particular.
This problem was solved by the successful isolation of the new strain (sis). This novel isolate designated by the present inventor as 88-KO-14-45 has been deposited at the Institute of Biotechnology, Institute of Industrial Science and Technology under the deposit number FERM P-14496, and has the following characteristics. Have

【0007】(1)この新規バチルス・チューリンゲン
シス(Bacillus thuringiensis)分離株88−KO−14
−45は、高知県桂浜の土壌より分離されたものであ
る。 (2)単離されたこの新規株は普通寒天培地(pH7.
6)上で、大きな粗面性白色コロニーを形成する。 (3)この細菌はグラム陽性の桿菌で鞭毛を有する。 (4)バチルス・チューリンゲンシス(Bacillus thurin
giensis)の分類は、その鞭毛の血清型により行われる
が、本発明の分離株はH抗原33型、Serovar leesisに
分類される。この血清型の菌は今までいかなる昆虫に対
してもその殺虫活性が示されていない。本発明の分離株
88−KO−14−45のオオチョウバエに対する活性
が、この血清型に分類され殺虫活性を示すバチルス・チ
ューリンゲンシスでの最初の報告である。 (5)本発明の新菌株は、酸素要求性、すなわち好気性
である。
(1) This novel Bacillus thuringiensis isolate 88-KO-14
-45 was isolated from the soil of Katsurahama, Kochi Prefecture. (2) The isolated new strain is agar medium (pH 7.
6) On top, form large rough white colonies. (3) This bacterium is a Gram-positive bacillus and has flagella. (4) Bacillus thurin
giensis) is classified according to the serotype of its flagella, but the isolate of the present invention is classified into H antigen type 33 and Serovar leesis. This serotype has not been shown to have any insecticidal activity against any insects. The activity of isolate 88-KO-14-45 of the present invention against Drosophila melanogaster is the first report in Bacillus thuringiensis that is serotyped and exhibits insecticidal activity. (5) The novel strain of the present invention is oxygen-requiring, that is, aerobic.

【0008】本発明の新規バチルス・チューリンゲンシ
ス(Bacillus thuringiensis) 分離株88−KO−14
−45は、不快衛生害虫のオオチョウバエ(Telmatoscop
us albipunctatus)幼虫に強い殺虫活性を示す。しかし
ながら、同じ双翅目昆虫のチカイエカ(Culex pipiens m
olestus)、ハマダラカ(Anopheles stephensi)、トクナ
ガユスリカ(Orthocladius akamusi)、イエバエ(Musuca
domestica)には本発明の新菌株は全く毒性を示さない。
このようにオオチョウバエのみに強い毒性を示し、他の
昆虫に毒性を示さない性質は環境に対する安全性を考慮
した場合特に有効である。
The novel Bacillus thuringiensis isolate 88-KO-14 of the present invention
-45 is an unpleasant hygiene pest, the fly flies (Telmatoscop)
us albipunctatus) shows a strong insecticidal activity against larvae. However, the same diptera, Culex pipiens m.
olestus), Anopheles stephensi, Orthocladius akamusi, Housefly (Musuca)
The new strain of the present invention shows no toxicity to domestica).
Thus, the property of showing strong toxicity only to Drosophila melanogaster and not showing toxicity to other insects is particularly effective in consideration of safety to the environment.

【0009】かくして、本発明に従えば、バチルス・チ
ューリゲンシス新菌株88−KO−14−45の培養物
を有効成分とする環境に優しいオオチョウバエ用殺虫剤
が提供される。新菌株88−KO−14−45の培養は
標準的な培地を用いて行うことができる。例えば、寒天
培地を用い、30℃で培養した場合、3日から5日で胞
子となり結晶性の毒素タンパクが産生される。有効成分
となる新規分離株88−KO−14−45の胞子と結晶
性毒素タンパクは寒天培地上からかき取ることにより回
収することができる。また、本発明の新規分離株は適当
な液体培地を用い培養することもでき、この場合には、
培養後に遠心分離もしくはろ過を行うことにより有効成
分を回収可能である。
Thus, according to the present invention, there is provided an environmentally friendly insecticide for fly flies, which contains a culture of Bacillus thuringiensis strain 88-KO-14-45 as an active ingredient. Cultivation of the new strain 88-KO-14-45 can be performed using a standard medium. For example, when cultured at 30 ° C. using an agar medium, spores are formed in 3 to 5 days and a crystalline toxin protein is produced. The spores and crystalline toxin protein of the novel isolate 88-KO-14-45, which are the active ingredients, can be recovered by scraping them from the agar medium. Further, the novel isolate of the present invention can also be cultivated using a suitable liquid medium, and in this case,
The active ingredient can be recovered by centrifugation or filtration after culturing.

【0010】本発明の新規分離株88−KO−14−4
5によって胞子形成期に産生される結晶性毒素タンパク
は、電気泳動測定(SDS−PAGE)したときに、3
1,62,66,72,および78キロダルトン付近に
強いラインを有し、さらに、55キロダルトン付近に弱
いラインを有するような分子量のものであることが見出
されている。
The novel isolate 88-KO-14-4 of the present invention
The crystalline toxin protein produced by 5 in the sporulation period was 3 when analyzed by electrophoresis (SDS-PAGE).
It has been found to be of molecular weight such that it has a strong line near 1,62,66,72, and 78 kilodaltons, and a weak line near 55 kilodaltons.

【0011】本発明の殺虫剤は、回収した新規分離株8
8−KO−14−45(胞子)もしくは結晶性毒素タン
パクを水に懸濁するか、もしくは適当な公知の配合剤を
加えることによって得た混合物として提供され、これを
対象害虫に摂食させることにより駆除を行う。殺虫剤混
合物中における有効成分の濃度は、一般に、胞子濃度と
して1×108 〜1010/mlである。
The insecticide of the present invention is a novel isolated strain 8
To provide 8-KO-14-45 (spore) or crystalline toxin protein as a mixture obtained by suspending in water or adding an appropriate known compounding agent, and feeding it to a target pest Disinfect by. The concentration of the active ingredient in the insecticide mixture is generally 1 × 10 8 to 10 10 / ml as the spore concentration.

【0012】[0012]

〔実施例1〕[Example 1]

〔新規バチルス・チューリンゲンシス分離株88−KO
−14−45の単離〕高知県桂浜の土壌0.5gを滅菌
水4.5mlを入れた試験管に秤取り、十分に懸濁させ
た。次にこの土壌懸濁液(10-1希釈液)を70゜Cの
水浴中で10分間放置し熱処理を行った。次に更に希釈
するため前記の土壌懸濁液を0.5ml取り、滅菌水
4.5mlに加え10-2希釈液を調整した。同様の方法
で10-5希釈液まで順次段階希釈液を調整した。
[New Bacillus thuringiensis isolate 88-KO
Isolation of 14-45] 0.5 g of the soil of Katsurahama, Kochi Prefecture was weighed in a test tube containing 4.5 ml of sterilized water and sufficiently suspended. Next, this soil suspension (10 -1 diluted solution) was left to stand in a 70 ° C water bath for 10 minutes for heat treatment. Next, for further dilution, 0.5 ml of the above soil suspension was taken and added to 4.5 ml of sterilized water to prepare a 10 -2 diluted solution. By the same method, serially diluted solutions were sequentially prepared up to 10 -5 diluted solutions.

【0013】次にこの希釈試料を普通寒天培地(肉エキ
ス10g、ポリペプトン10g、塩化ナトリウム2g、
寒天20g、水1リットル、pH7.6)に0.1ml
塗抹し、28゜Cで3日から5日間培養した。所定期間
培養後生育してきたコロニーを位相差顕微鏡下で観察
し、白色に観察される胞子と、黒色に観察される結晶タ
ンパクが同一細胞中に認められたものを選択した。
Next, this diluted sample was added to an ordinary agar medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g,
Agar 20 g, water 1 liter, pH 7.6) 0.1 ml
It was smeared and cultured at 28 ° C for 3 to 5 days. Colonies that had grown after culturing for a predetermined period were observed under a phase contrast microscope, and spores observed in white and crystal proteins observed in black were found in the same cell, and selected.

【0014】〔実施例2〕 〔新規バチルス・チューリンゲンシス分離株88−KO
−14−45の血清型の同定〕血清型の同定は、大庭ら
の方法(Ohba,M. : J. Invertebr. Pathol. 32, 303-309
(1978))で行った。本発明の新規Bacillus thuringiens
is分離株88−KO−14−45をL型試験管中の4m
lの培地(肉エキス10g、ポリペプトン10g、塩化
ナトリウム2g、水1リットル、pH7.6)に懸濁
し、37゜Cで3〜4時間、静かに振とうした。3〜4
時間後、この懸濁液1滴を、スライドガラス上で、20
〜50倍に希釈した血清(No.1〜34)1滴と混合
した。3〜5分後に凝集反応を判定し、血清型33の血
清にのみ反応を示したことから、血清型33のリーシス
と決定した。
Example 2 [Novel Bacillus thuringiensis isolate 88-KO
Identification of serotype of 14-45] The serotype was identified by Ohba et al. (Ohba, M .: J. Invertebr. Pathol. 32, 303-309).
(1978)). Novel Bacillus thuringiens of the present invention
is isolate 88-KO-14-45 in L type test tube 4m
It was suspended in 1 liter of medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g, water 1 liter, pH 7.6) and gently shaken at 37 ° C. for 3 to 4 hours. 3-4
After a period of time, 1 drop of this suspension was placed on a glass slide for 20
Mixed with 1 drop of serum (No. 1-34) diluted to 50 times. The agglutination reaction was judged after 3 to 5 minutes, and it showed to react only with the serum of serotype 33, so it was determined to be lysis of serotype 33.

【0015】〔実施例3〕 〔双翅目昆虫に対する毒性試験〕本発明の新規バチルス
・チューリンゲンシス分離株88−KO−14−45を
普通寒天培地で28゜Cで5日間培養後集菌し、蒸留水
で2回から3回洗浄した。オオチョウバエ(Telmatoscop
us albipunctatus) 、チカイエカ(Culex pipiensmolest
us)、ハマダラカ(Anopheles stephensi)、トクナガユス
リカ(Orthocladius akamusi)については、胞子濃度が1
8 /mlとなるように培養物を蒸留水に懸濁し、この
中にそれぞれの昆虫の幼虫5匹を入れ、25゜Cで3日
間試験した。またイエバエ(Musuca domestica)について
は、幼虫1匹あたりが108 個の胞子を食餌するよう
に、餌の中に培養物を混入し、同様に幼虫5匹に対し2
5゜Cで3日間試験した。なお結晶性タンパクは、胞子
形成の際、1細胞から1個、もしくはそれ以上形成され
る。
[Example 3] [Toxicity test against Diptera insects] The novel Bacillus thuringiensis isolate 88-KO-14-45 of the present invention was cultured on ordinary agar medium at 28 ° C for 5 days and then collected. , Washed with distilled water 2 to 3 times. Flycatcher (Telmatoscop
us albipunctatus), Culex pipiensmolest
us), Anopheles stephensi, and Orthocladius akamusi have a spore concentration of 1
The 0 8 / ml and made as culture was suspended in distilled water, placed in a 5 larvae each insect therein were tested for 3 days at 25 ° C. As for the housefly (Musuca domestica), the culture was mixed in the diet so that each larva feeds 10 8 spores.
Tested at 5 ° C for 3 days. It should be noted that one or more crystalline proteins are formed from one cell during sporulation.

【0016】毒性試験の結果を表1に示す。また、活性
のあったオオチョウバエに対しては様々な濃度に希釈し
た培養物懸濁液に、幼虫20頭を入れ、24時間後の死
虫数を測定し、プロビット法によりLC50を求めた。
その結果LC50値は217μg wet weigt /mlであっ
た。本実施例より、本発明の新規分離株88−KO−1
4−45は、双翅目昆虫の中でオオチョウバエに特異的
に殺虫活性を有する事が明かとなった。
The results of the toxicity test are shown in Table 1. For active Drosophila melanogaster, 20 larvae were placed in a culture suspension diluted to various concentrations, the number of dead larvae was measured after 24 hours, and LC50 was determined by the probit method.
As a result, the LC50 value was 217 μg wet weigt / ml. From this example, the novel isolate 88-KO-1 of the present invention is obtained.
It has been revealed that 4-45 has a specific insecticidal activity against Drosophila among Diptera insects.

【0017】[0017]

【表1】 [Table 1]

【0018】〔実施例4〕 〔新規分離株88−KO−14−45の産生する結晶性
毒素タンパクの精製〕本発明の新規分離株88−KO−
14−45を普通寒天培地(肉エキス10g、ポリペプ
トン10g、塩化ナトリウム2g、寒天20g、水1リ
ットル、pH7.6)で27゜C、5日間培養後、集菌
し、1M塩化ナトリウム溶液で洗浄、遠心を3回行っ
た。結晶性毒素タンパクの部分精製は、硫酸デキストラ
ン−ポリエチレングリコール系による二相分離法(Goodm
an. N. S. : J. Bact. 94, 485(1967))により行った。
結晶性毒素タンパクを含む下層を水で希釈後、遠心して
集めた。さらに純粋になるまで精製を行うために、ショ
糖密度勾配遠心法を行った。
[Example 4] [Purification of crystalline toxin protein produced by novel isolate 88-KO-14-45] Novel isolate 88-KO- of the present invention
14-45 was cultured on a regular agar medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g, agar 20 g, water 1 liter, pH 7.6) at 27 ° C. for 5 days, then collected and washed with 1M sodium chloride solution. The centrifugation was performed 3 times. The crystalline toxin protein can be partially purified by a two-phase separation method using the dextran sulfate-polyethylene glycol system (Goodm
an. NS: J. Bact. 94, 485 (1967)).
The lower layer containing the crystalline toxin protein was diluted with water and then collected by centrifugation. Sucrose density gradient centrifugation was performed to further purify the product.

【0019】70W/V%、80W/V%、90W/V
%のショ糖溶液を10ml容遠心管に重層し、さらに上
部に部分精製した結晶性毒素タンパクを0.5ml重層
した。22,000rpmで2時間遠心を行い、70W
/V%−80W/V%界面に沈降する、純粋に精製され
た結晶性毒素タンパクを回収した。この結晶性毒素タン
パクをSDS−PAGEにかけたところ、31,62,
66,72,および78キロダルトン付近に強いらライ
ンが現われ、また55キロダルトン付近に弱いラインが
認められた。
70 W / V%, 80 W / V%, 90 W / V
% Sucrose solution was layered on a 10 ml centrifuge tube, and 0.5 ml of partially purified crystalline toxin protein was layered on top. Centrifuge at 22,000 rpm for 2 hours, 70W
Purely purified crystalline toxin protein was recovered which precipitated at the / V% -80W / V% interface. When this crystalline toxin protein was subjected to SDS-PAGE, 31,62,
A strong line appeared near 66, 72, and 78 kilodaltons, and a weak line was observed near 55 kilodaltons.

【0020】[0020]

【発明の効果】本発明の新規分離株88−KO−14−
45は、家庭用の簡易浄化槽や下水処理槽等で発生する
不快衛生害虫のオオチョウバエ(Telmatoscopus albipun
ctatus) に対して特異的な殺虫活性を有しており、この
害虫の駆除に有効利用できる。
The novel isolate 88-KO-14 of the present invention
45 is a bot fly (Telmatoscopus albipun), which is an unpleasant hygienic pest that occurs in simple household septic tanks and sewage treatment tanks.
It has a specific insecticidal activity against ctatus) and can be effectively used for exterminating this pest.

Claims (2)

【特許請求の範囲】[Claims] 【請求項1】 オオチョウバエに対して特異的に殺虫活
性を有するバチルス・チューリンゲンシス新菌株88−
KO−14−45(生命工学工業技術研究所受託番号F
ERM P−14496)。
1. A new strain of Bacillus thuringiensis 88- having a specific insecticidal activity against Drosophila melanogaster.
KO-14-45 (Contract No. F, Institute of Life Science and Technology)
ERM P-14496).
【請求項2】 バチルス・チューリンゲンシス新菌株8
8−KO−14−45(生命工学工業技術研究所受託番
号FERM P−14496)の培養物を有効成分とす
るオオチョウバエ用殺虫剤。
2. A new Bacillus thuringiensis strain 8
An insecticide for Drosophila melanogaster, which comprises a culture of 8-KO-14-45 (Contract No. FERM P-14496, Institute of Life Science and Technology) as an active ingredient.
JP26151594A 1994-09-29 1994-09-29 Bacillus thuringiensis new strain Expired - Fee Related JP3251137B2 (en)

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JP3251137B2 JP3251137B2 (en) 2002-01-28

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