JPH0870625A - Improvement of germination of hard seed - Google Patents
Improvement of germination of hard seedInfo
- Publication number
- JPH0870625A JPH0870625A JP20977894A JP20977894A JPH0870625A JP H0870625 A JPH0870625 A JP H0870625A JP 20977894 A JP20977894 A JP 20977894A JP 20977894 A JP20977894 A JP 20977894A JP H0870625 A JPH0870625 A JP H0870625A
- Authority
- JP
- Japan
- Prior art keywords
- germination
- seeds
- seed
- sulfuric acid
- hard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、胚をとりまく構造物が
硬い種皮や果被で覆われている種子、すなわち、硬実種
子の発芽改良方法に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a seed for which a structure surrounding an embryo is covered with a hard seed coat or a fruit cover, that is, a method for improving germination of a hard seed.
【０００２】[0002]
【従来の技術】アオイ科のワタ、オクラ、ヒルガオ科の
アサガオ、ヒルガオ、ユリ科のアスパラガス、アカザ科
のビート、ホウレンソウ、キク科のゴボウ、シュンギ
ク、ヒマワリ、タデ科のソバ、フクロウソウ科のゼラニ
ウム、カヤツリグサ科のコウボウムギ、マメ科のユーカ
リ、サブクローバー、ウリ科のカボチャ、スイカ等の種
子は、外層に厚く堅固な種皮や果被を有しており、発芽
吸水時に発芽に必要な吸水に長い時間を要し、そのため
各種子の発芽の時期が一定でなく、発芽の揃いが悪くな
ることが知られている。特にホウレンソウやビートの場
合、過水分条件下では、果被が一気に吸水をしてしまう
ことによって、一時的に胚への酸素の供給が絶たれてし
まう。このため、酸素要求性が高い発芽への悪影響が生
じ、種子の発芽不良を起こす現象が知られている。また
ホレンソウは、高温時になると種子の発芽性能が低下す
る性質を持っており、周年栽培における、夏場の高温時
に種子を播種する場合には、出芽率の低下、不揃いの発
生等といった問題が生じている。このため、上記のよう
な過水分条件下の土壌や高温等の悪条件下でも種子の発
芽が安定するような発芽改良方法が必要となってきた。
特開平３−１２３４０４号公報に示されたように、果被
を除去することによって発芽を改良する方法〔果被除去
種子（通称、ネーキッド種子）の利用〕はその一例であ
る。また特開平２−２８３２０１号公報に示されている
ように、機械的方法で果被を部分的に破壊する方法も試
みられている。2. Description of the Related Art Cotton of the mallow family, okra, morning glory of the family Convolvulaceae, bindweed, asparagus of the family Liliaceae, beet of the family Aceraceae, spinach, burdock of the Asteraceae, Sungiku, sunflower, buckwheat of the Polygonaceae, of the owl family Seeds such as geranium, Cyperaceae, eucalyptus of the family Cyperaceae, eucalyptus of the leguminous family, subclover, pumpkin of the family Cucurbitaceae, watermelon, etc. have a thick and firm seed coat and fruit cover in the outer layer. It takes a long time, and it is known that the germination time of each offspring is not constant and the germination becomes poor. In the case of spinach and beet, in particular, under super-moisture conditions, the fruit cover absorbs water all at once, which temporarily interrupts the supply of oxygen to the embryo. For this reason, it is known that germination, which has a high oxygen requirement, is adversely affected and causes poor germination of seeds. In addition, spinach has the property that the germination performance of seeds decreases at high temperatures, and when seeds are sown at high temperatures in the summer in the year-round cultivation, problems such as a lower emergence rate and occurrence of unevenness occur. There is. For this reason, there has been a need for a germination improving method capable of stabilizing the germination of seeds even under adverse conditions such as soil and high temperature under the above-mentioned excessive moisture condition.
As disclosed in JP-A-3-123404, a method of improving germination by removing a fruit cover [use of a fruit cover-removed seed (commonly known as naked seed)] is one example. Further, as disclosed in JP-A-2-283201, a method of partially destroying the fruit cover by a mechanical method has been attempted.
【０００３】[0003]
【発明が解決しようとする課題】しかしながら、果被を
除去することによって発芽を改良する方法は、果被を完
全に取り除いているため、種子によっては発芽性能は非
常に良い反面、胚の保護といった果被が負うべき本来の
機能も取り除かれているために、立ち枯れ等、その後の
生育に問題を生じるケースが多々見られ、実用上難があ
るのが現状である。また、機械的方法では、果被を均一
に脆弱化することは難しい。このため、果被の脆弱化が
均一でない種子を発芽させると、一部脆弱化された箇所
から、子葉が先に突出するといった異常発芽を生じるこ
とがあり、発芽の均一性が乱れる原因となる。However, the method of improving germination by removing the fruit cover completely removes the fruit cover, so that depending on the seed, the germination performance is very good, but the germ protection is not effective. Since the original function of the fruit cover has been removed, there are many cases in which there is a problem in the subsequent growth, such as withering and death, and it is currently a practical problem. Further, it is difficult to uniformly weaken the fruit cover by a mechanical method. Therefore, germination of seeds whose fruit cover is not evenly weakened may cause abnormal germination such that the cotyledon protrudes first from the weakened part, which causes the uneven germination. .
【０００４】[0004]
【課題を解決するための手段】このような状況下で、本
発明者らは鋭意検討を行った結果、比較的低い濃度の硫
酸に果被を浸漬させることにより果被を脆弱化させ、さ
らにヒドロキシアミンまたはヒドロキシ尿素による浸漬
処理を行うことによって、広い温度範囲において発芽速
度、発芽率共に充分に満足できる種子を製造できること
を見い出し本発明を完成させた。すなわち、本発明は、
硬実種子の発芽改良方法において、（１）種子を０．５
Ｎ〜１８Ｎの硫酸に０℃〜６０℃下で、０．５時間〜８
時間浸漬処理する工程（以下、硫酸処理と記す。）、
（２）前記工程で浸漬処理された種子をヒドロキシアミ
ンまたはヒドロキシ尿素の水溶液に１５℃〜３５℃下
で、１時間〜５日間浸漬処理する工程（以下、アミン処
理と記す。）、から実質的になることを特徴とする硬実
種子の発芽改良方法（以下、本発明方法と記す。）、及
び発芽改良方法によって製造された発芽改良硬実種子
（以下、本発明種子と記す。）を提供するものである。Under the circumstances, as a result of intensive investigations by the present inventors, as a result of dipping the fruit cover in sulfuric acid having a relatively low concentration, the fruit cover is weakened, The present invention has been completed by finding that seeds having a sufficient germination rate and germination rate can be produced in a wide temperature range by performing a dipping treatment with hydroxyamine or hydroxyurea. That is, the present invention is
In the method for improving germination of hard seeds, (1) 0.5 seeds are used.
0.5 to 8 hours under N to 18N sulfuric acid at 0 to 60 ° C
A step of performing a time immersion treatment (hereinafter referred to as sulfuric acid treatment),
(2) Substantially from the step of immersing the seeds subjected to the immersion treatment in the above step in an aqueous solution of hydroxyamine or hydroxyurea at 15 ° C to 35 ° C for 1 hour to 5 days (hereinafter referred to as amine treatment). And a germination-improved solid seed (hereinafter referred to as the seed of the present invention) produced by the germination-improved method. To do.
【０００５】以下、さらに詳細に本発明を説明する。本
発明は、胚をとりまく構造物が硬い種皮や果被で覆われ
ている種子の発芽率及び発芽速度を向上させる方法に関
するものである。本発明において適用できる種子として
は、例えば、アオイ科のワタ、オクラ、ヒルガオ科のア
サガオ、ヒルガオ、ユリ科のアスパラガス、アカザ科の
ビート、ホウレンソウ、キク科のゴボウ、シュンギク、
ヒマワリ、タデ科のソバ、フクロウソウ科のゼラニウ
ム、カヤツリグサ科のコウボウムギ、マメ科のユーカ
リ、サブクローバー、ウリ科のカボチャ、スイカ等の胚
をとりまく構造物が硬い種皮や果被で覆われているよう
な種子をあげることができる。The present invention will be described in more detail below. TECHNICAL FIELD The present invention relates to a method for improving the germination rate and the germination rate of seeds in which the structure surrounding the embryo is covered with a hard seed coat or fruit cover. The seeds applicable in the present invention include, for example, cotton in the mallow family, okra, morning glory in the family Convolvulaceae, bindweed, asparagus in the lily family, beetroot in the family Rhinoceros, spinach, burdock in the Asteraceae family, chrysanthemum,
The structure surrounding embryos such as sunflower, buckwheat in the Polygonaceae, geranium in the Oleaceae, Kobomugi in the family Cyperaceae, eucalyptus in the leguminous, subclover, squash in the Cucurbitaceae, watermelon, etc. is covered with a hard seed coat and fruit cover. You can raise such seeds.
【０００６】本発明方法の第１段目の工程である硫酸処
理では、硫酸濃度が０．５Ｎ〜１８Ｎの範囲である硫酸
を使用することが必要である。そしてその処理時間は
０．５時間〜８時間の範囲である。これらの処理条件を
はずれる場合、例えば、硫酸濃度が低い、もしくは、処
理時間が短かい場合には、効果がない。一方、硫酸濃度
が高い、もしくは、処理時間が長い場合には、胚にダメ
ージが与えてしまう。さらに処理温度は、通常、０℃〜
６０℃の範囲である。より好ましくは約２０℃〜４０℃
の範囲をあげることができる。In the sulfuric acid treatment which is the first step of the method of the present invention, it is necessary to use sulfuric acid having a sulfuric acid concentration in the range of 0.5N to 18N. The processing time is in the range of 0.5 hours to 8 hours. If these treatment conditions are deviated, for example, if the sulfuric acid concentration is low or the treatment time is short, there is no effect. On the other hand, when the concentration of sulfuric acid is high or the treatment time is long, the embryo is damaged. Further, the processing temperature is usually 0 ° C to
It is in the range of 60 ° C. More preferably about 20 ° C to 40 ° C
The range can be raised.
【０００７】本発明方法の第２段目の工程であるアミン
処理では、前記工程で浸漬処理された種子をヒドロキシ
アミンまたはヒドロキシ尿素の水溶液に１５℃〜３５℃
下で、１時間〜５日間浸漬処理することが必要である。
処理濃度として、たとえば、約１００ｐｐｍから約５０
００ｐｐｍの範囲があげられる。In the amine treatment, which is the second step of the method of the present invention, the seeds soaked in the above step are immersed in an aqueous solution of hydroxyamine or hydroxyurea at 15 ° C to 35 ° C.
It is necessary to perform the immersion treatment for 1 hour to 5 days under.
As the treatment concentration, for example, about 100 ppm to about 50
The range is 00 ppm.
【０００８】また本発明方法は、硬実種子の発芽改良方
法において、（１）種子を０．５Ｎ〜１８Ｎの硫酸に０
℃〜６０℃下で、０．５時間〜８時間浸漬処理する工
程、（２）前記工程で処理された種子をヒドロキシアミ
ンまたはヒドロキシ尿素の水溶液に１５℃〜３５℃下
で、１時間〜５日間浸漬処理する工程、から実質的にな
ることを特徴とする硬実種子の発芽改良方法であるが、
他の工程として、第１工程と第２工程の間に、種子を３
０℃〜７０℃の範囲で、相対湿度６０％以下の空気を４
時間〜２４時間、より好ましくは６時間〜１６時間の範
囲で通気処理する工程や硫酸または硫酸・通風処理され
た種子から、例えば、流水中に数時間程度以上、好まし
くは約５時間以上種子をさらして硫酸を除去処理する工
程、静置水に種子を浸して水酸化ナトリウム、炭酸カル
シウム、炭酸水素ナトリウム等のアルカリで中和しなが
ら酸を取り除く処理をする工程、殺菌剤、殺虫剤等の農
薬、発芽促進剤等の植物成長調節剤等を処理する工程も
加えることもできる。このようにしてよりすぐれた発芽
性能の付与、種子の発芽促進や病害虫の予防をすること
も可能である。以下、本発明を実施例によってさらに詳
しく説明するが、本発明は以下の実施例のみに限定され
るものではない。The method of the present invention is a method for improving germination of hard seeds, wherein (1) seeds are dissolved in 0.5 N to 18 N sulfuric acid.
A step of dipping at 0.5 to 60 ° C. for 0.5 to 8 hours, and (2) an aqueous solution of hydroxyamine or hydroxyurea at 15 ° to 35 ° C. for 1 to 5 hours. A method of improving germination of hard seeds, which is characterized by consisting essentially of a step of immersion treatment for a day,
As another step, 3 seeds are added between the first step and the second step.
Air with a relative humidity of 60% or less in the range of 0 ° C to 70 ° C
From the step of aeration treatment in the range of time to 24 hours, more preferably 6 hours to 16 hours, or seeds that have been treated with sulfuric acid or sulfuric acid / ventilation, for example, seeds in running water for about several hours or more, preferably for about 5 hours or more A step of removing sulfuric acid by exposing, a step of soaking seeds in static water to remove acid while neutralizing with an alkali such as sodium hydroxide, calcium carbonate, sodium hydrogen carbonate, bactericide, insecticide, etc. It is also possible to add a step of treating a plant growth regulator such as an agricultural chemical or a germination accelerator. In this way, it is also possible to impart better germination performance, promote seed germination and prevent pests. Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples.
【０００９】[0009]
実施例１ コウボウムギ種子を０．１Ｎ（比較用）、０．５Ｎ、
１．０Ｎ、２．０Ｎ、４．０Ｎ、９．０Ｎ、１８．０
Ｎ、２０．０Ｎ（比較用）または２５．０Ｎ（比較用）
の硫酸に３０℃下、４時間浸漬した後、炭酸水素ナトリ
ウムの飽和溶液で中和した。さらに得られた種子を１０
００ｐｐｍのヒドロキシアミンまたはヒドロキシ尿素の
水溶液に３０℃下で、３日間（暗条件）浸漬した。Example 1 0.1 N (for comparison), 0.5 N
1.0N, 2.0N, 4.0N, 9.0N, 18.0
N, 20.0N (for comparison) or 25.0N (for comparison)
After immersing in sulfuric acid of 30 ° C. for 4 hours at 30 ° C., it was neutralized with a saturated solution of sodium hydrogen carbonate. Furthermore, 10 seeds obtained
It was dipped in an aqueous solution of 00 ppm of hydroxyamine or hydroxyurea at 30 ° C. for 3 days (dark condition).
【００１０】試験例１ 実施例１によって得られた種子と比較のための無処理種
子を変温条件で発芽試験（８時間、暗条件、１５℃：１
６時間、明条件、３０℃）を行った。その結果を表１及
び表２に示す。なお、本発明種子では、発芽直後から本
葉展開期の植物体になんらの異常性も認められなかった
が、比較例〔硫酸濃度（Ｎ）２０．０、２５．０〕では
異常出芽が５０％程度認められた。Test Example 1 The seeds obtained in Example 1 and untreated seeds for comparison were subjected to a germination test under varying temperature conditions (8 hours, dark conditions, 15 ° C .: 1 ° C.).
Light conditions, 30 ° C.) were performed for 6 hours. The results are shown in Tables 1 and 2. In the seeds of the present invention, no abnormalities were observed in the plants in the true leaf development stage immediately after germination, but in the comparative example [sulfate concentration (N) 20.0, 25.0], abnormal germination was 50. % Was recognized.
【００１１】[0011]
【表１】 試験条件：ヒドロキシアミン ────────────────────────────── 供試種子 硫酸濃度 発芽率〔％〕 （Ｎ） ────────────────── １２ １４ ２１ （発芽日） ────────────────────────────── 無処理 − ０ ０ ０ 比較例 ０．１ ０ ０ １３ 本発明 ０．５ １０ ３４ ５８ 本発明 １．０ ８ ４２ ６３ 本発明 ２．０ １２ ３６ ５９ 本発明 ４．０ ２８ ４４ ７０ 本発明 ９．０ ３０ ６０ ６９ 本発明 １８．０ ３８ ５９ ７３ 比較例 ２０．０ １０ ２３ ３８ 比較例 ２５．０ ３２ ４８ ５０ ──────────────────────────────[Table 1] Test conditions: Hydroxyamine ────────────────────────────── Test seeds Sulfuric acid concentration Germination rate [%] ( N) ────────────────── 12 14 21 (germination date) ──────────────────────── ─────── No treatment − 0 0 0 Comparative example 0.1 0 0 13 Invention 0.5 10 34 58 Invention 1.0 8 42 63 Invention 2.0 12 36 59 Invention 4.0 28 44 70 Present invention 9.0 30 60 69 Present invention 18.0 38 59 73 Comparative example 20.0 10 23 38 Comparative example 25.0 32 48 50 50 ──────────────── ───────────────
【００１２】[0012]
【表２】 試験条件：ヒドロキシ尿素 ────────────────────────────── 供試種子 硫酸濃度 発芽率〔％〕 （Ｎ） ────────────────── １２ １４ ２１ （発芽日） ────────────────────────────── 無処理 − ０ ０ ０ 比較例 ０．１ ０ １３ ２８ 本発明 ０．５ ２０ ４３ ７２ 本発明 １．０ １８ ５２ ８０ 本発明 ２．０ ４３ ７９ ８８ 本発明 ４．０ ５０ ６２ ８３ 本発明 ９．０ ４８ ７７ ８６ 本発明 １８．０ ６２ ８３ ９０ 比較例 ２０．０ ２０ ３６ ４５ 比較例 ２５．０ ３８ ４４ ５３ ──────────────────────────────[Table 2] Test conditions: hydroxyurea ────────────────────────────── Test seeds Sulfuric acid concentration Germination rate [%] ( N) ────────────────── 12 14 21 (germination date) ──────────────────────── ─────── No treatment − 0 0 0 Comparative example 0.1 0 13 28 Invention 0.5 20 43 72 Invention 1.0 18 52 80 Invention 2.0 43 79 88 Invention 4.0 50 62 83 The present invention 9.0 48 77 78 The present invention 18.0 62 83 90 Comparative example 20.0 20 36 45 Comparative example 25.0 38 44 53 53 ──────────────── ───────────────
【００１３】比較例１ コウボウムギ種子を１８．０Ｎの硫酸に３０℃下、４時
間浸漬した後、炭酸水素ナトリウムの飽和溶液で中和す
ることによって比較用種子を得た（比較例１）。Comparative Example 1 Kobomugi seeds were immersed in 18.0 N sulfuric acid at 30 ° C. for 4 hours and then neutralized with a saturated solution of sodium hydrogen carbonate to obtain comparative seeds (Comparative Example 1).
【００１４】比較例２ コウボウムギ種子を１０００ｐｐｍのヒドロキシアミン
またはヒドロキシ尿素の水溶液に３０℃下で、３日間
（暗条件）浸漬することによって比較用種子を得た（比
較例２ａ，２ｂ）。Comparative Example 2 Comparative seeds were obtained by immersing Kobomugi seeds in 1000 ppm of an aqueous solution of hydroxyamine or hydroxyurea at 30 ° C. for 3 days (dark conditions) (Comparative Examples 2a and 2b).
【００１５】試験例２ 比較例１及び２によって得られた種子と無処理種子を変
温条件で発芽試験（８時間、暗条件、１５℃：１６時
間、明条件、３０℃）を行った。その結果を表１及び表
２に示す。なお、本発明種子では、発芽直後から本葉展
開期の植物体になんらの異常性も認められなかった。Test Example 2 The seeds obtained in Comparative Examples 1 and 2 and untreated seeds were subjected to a germination test (8 hours, dark conditions, 15 ° C .: 16 hours, bright conditions, 30 ° C.) under varying temperature conditions. The results are shown in Tables 1 and 2. In addition, in the seeds of the present invention, no abnormality was observed in the plants at the stage of developing true leaves immediately after germination.
【００１６】[0016]
【表３】 ──────────────────────────────────── 供試種子 硫酸濃度 供試薬剤 発芽率〔％〕 （Ｎ） ─────────────────── １２ １４ ２１ （発芽日） ──────────────────────────────────── 無処理 − − ０ ０ ０ 実施例１ １８．０ ヒドロキシアミン ３８ ５９ ７３ 実施例１ １８．０ ヒドロキシ 尿素 ６２ ８３ ９０ 比較例１ １８．０ − ５ １６ ２２ 比較例２ − ヒドロキシアミン ０ ２ ７ 比較例２ − ヒドロキシ 尿素 ０ ９ １１ ────────────────────────────────────[Table 3] ──────────────────────────────────── Test seeds Sulfuric acid concentration Test reagents Germination rate [%] (N) ─────────────────── 12 14 21 (germination date) ─────────────────── ────────────────── Untreated --- 0 0 0 Example 1 18.0 Hydroxyamine 38 59 73 Example 1 18.0 Hydroxy urea 62 83 90 Comparative Example 1 18.0-5 16 22 Comparative Example 2-Hydroxyamine 0 27 7 Comparative Example 2-Hydroxyurea 0 9 11 ────────────────────────── ───────────
【００１７】[0017]
【発明の効果】本発明により、過剰水分等の悪条件下で
も安定した高い発芽性能（発芽速度、発芽率）を有する
硬実種子を製造することが可能になった。Industrial Applicability According to the present invention, it has become possible to produce solid seeds having stable and high germination performance (germination rate, germination rate) even under adverse conditions such as excess water.
Claims (2)
種子を０．５Ｎ〜１８Ｎの硫酸に０℃〜６０℃下で、
０．５時間〜８時間浸漬処理する工程、（２）前記工程
で処理された種子をヒドロキシアミンまたはヒドロキシ
尿素の水溶液に１５℃〜３５℃下で、１時間〜５日間浸
漬処理する工程、から実質的になることを特徴とする硬
実種子の発芽改良方法。1. A method for improving germination of hard seeds, which comprises (1)
Seeds in 0.5N to 18N sulfuric acid at 0 ° C to 60 ° C,
From the step of soaking for 0.5 to 8 hours, (2) the step of soaking the seed treated in the above step in an aqueous solution of hydroxyamine or hydroxyurea at 15 to 35 ° C for 1 to 5 days A method for improving germination of hard seeds, which is substantially the same.
された発芽改良硬実種子。2. A germination-improved hard seed produced by the germination-improvement method according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20977894A JPH0870625A (en) | 1994-09-02 | 1994-09-02 | Improvement of germination of hard seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20977894A JPH0870625A (en) | 1994-09-02 | 1994-09-02 | Improvement of germination of hard seed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0870625A true JPH0870625A (en) | 1996-03-19 |
Family
ID=16578456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20977894A Pending JPH0870625A (en) | 1994-09-02 | 1994-09-02 | Improvement of germination of hard seed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0870625A (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102282928A (en) * | 2011-06-14 | 2011-12-21 | 中国科学院新疆生态与地理研究所 | Artificial method for physical dormancy release of Zunghar leaf-free bean seeds |
| CN102783341A (en) * | 2012-08-21 | 2012-11-21 | 南阳师范学院 | Method for improving germination percentage of paspalum natatum in warm temperate zones |
| CN104160805A (en) * | 2014-09-02 | 2014-11-26 | 连云港西诺花卉种业有限公司 | Germination accelerating and rooting method for lily bulbs |
| CN108934263A (en) * | 2018-05-03 | 2018-12-07 | 广西壮族自治区药用植物园 | Break the method for culturing seedlings of tinosporae seed dormancy |
| CN114885631A (en) * | 2022-04-06 | 2022-08-12 | 湖北瑞晟农业科技有限责任公司 | Shell breaking and germination accelerating method for hard shell plant seeds |
-
1994
- 1994-09-02 JP JP20977894A patent/JPH0870625A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102282928A (en) * | 2011-06-14 | 2011-12-21 | 中国科学院新疆生态与地理研究所 | Artificial method for physical dormancy release of Zunghar leaf-free bean seeds |
| CN102783341A (en) * | 2012-08-21 | 2012-11-21 | 南阳师范学院 | Method for improving germination percentage of paspalum natatum in warm temperate zones |
| CN104160805A (en) * | 2014-09-02 | 2014-11-26 | 连云港西诺花卉种业有限公司 | Germination accelerating and rooting method for lily bulbs |
| CN108934263A (en) * | 2018-05-03 | 2018-12-07 | 广西壮族自治区药用植物园 | Break the method for culturing seedlings of tinosporae seed dormancy |
| CN114885631A (en) * | 2022-04-06 | 2022-08-12 | 湖北瑞晟农业科技有限责任公司 | Shell breaking and germination accelerating method for hard shell plant seeds |
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