JPH08165298A - Production of water soluble protein - Google Patents
Production of water soluble proteinInfo
- Publication number
- JPH08165298A JPH08165298A JP33162994A JP33162994A JPH08165298A JP H08165298 A JPH08165298 A JP H08165298A JP 33162994 A JP33162994 A JP 33162994A JP 33162994 A JP33162994 A JP 33162994A JP H08165298 A JPH08165298 A JP H08165298A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- aqueous solution
- sodium
- reducing agent
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- Peptides Or Proteins (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、水に不溶な蛋白質から
なる原料を用いて、簡単に、かつ高収率で、可溶性蛋白
質を製造する方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a soluble protein simply and in high yield using a raw material composed of a water-insoluble protein.
【０００２】[0002]
【従来の技術・発明が解決しようとする課題】生体の構
成成分である蛋白質には水に不溶性のものが多く見られ
る。例えば、羽根、毛、爪、角、皮、肉や魚鱗等の構成
成分である動物由来の蛋白質や絹等の昆虫由来の蛋白質
あるいは大豆や小麦等の植物由来の蛋白質等が挙げられ
る。これらは水に溶けないか、あるいは溶けにくいもの
の、自然界では生物の有する蛋白分解酵素により分解さ
れることにより可溶化されている。近年ではこのような
水に溶けにくい蛋白質を可溶化したものが、例えば化粧
品や食品の添加物を始め、衣料や塗料の改質剤や重金属
類の捕捉剤等として利用されており、また新しい用途が
期待されている。2. Description of the Related Art Many proteins, which are constituents of living organisms, are insoluble in water. Examples thereof include animal-derived proteins that are constituents of feathers, hair, nails, horns, skins, meat and fish scales, insect-derived proteins such as silk, and plant-derived proteins such as soybeans and wheat. Although these are insoluble or hardly soluble in water, they are solubilized in nature by being decomposed by proteolytic enzymes of organisms. In recent years, solubilized proteins that are difficult to dissolve in water have been used as additives for cosmetics and foods, modifiers for clothing and paints, scavengers for heavy metals, etc., as well as new applications. Is expected.
【０００３】これらの蛋白質を化学的な方法で可溶化す
るには、酸あるいはアルカリで処理する方法、還元剤あ
るいは酸化剤を用いてペプチド結合を切断する方法等が
挙げられる（高分子加工、山内清、４３巻１号（1994
年）p.14〜19）。しかし、これらの方法の幾つかは不溶
解物が多く出るために収率が低かったり、強酸や強アル
カリを高温で用いるために危険であったり、高濃度の酸
化剤や重金属等の薬品を用いたり環境に影響を及ぼすハ
ロゲン系溶剤等を用いたりしているために製造廃液の処
理に手間がかかり、安価に製造するためには解決すべき
種々の問題が残されている。従って、本発明の目的は、
製造に関わる排水処理を容易にするため過量の酸化剤や
還元剤あるいは有機溶媒や重金属等を用いることなく、
水に溶解しない蛋白質から、短時間かつ高収率で水溶性
の蛋白質を製造する方法を提供することにある。また、
本発明の他の目的は、こうして得られた可溶性蛋白質液
を再度還元処理を行うことによるシスチン含有水溶性蛋
白質の製造法を提供することにある。To solubilize these proteins by a chemical method, a method of treating with an acid or an alkali, a method of cleaving a peptide bond with a reducing agent or an oxidizing agent, and the like (polymer processing, Yamauchi) Kiyoshi, Vol. 43, No. 1 (1994
Year) p.14-19). However, some of these methods have low yields due to large amounts of insoluble matter, are dangerous because they use strong acids or strong alkalis at high temperatures, and use high concentrations of oxidizers and chemicals such as heavy metals. In addition, since a halogen-based solvent or the like that affects the environment is used, it takes a lot of time to process the manufacturing waste liquid, and various problems remain to be solved in order to manufacture at low cost. Therefore, the object of the present invention is to
To facilitate wastewater treatment related to production, without using excessive amounts of oxidizing agents and reducing agents, organic solvents, heavy metals, etc.
It is an object of the present invention to provide a method for producing a water-soluble protein in a short time and in a high yield from a protein that is insoluble in water. Also,
Another object of the present invention is to provide a method for producing a cystine-containing water-soluble protein by subjecting the thus-obtained soluble protein solution to a reduction treatment again.
【０００４】[0004]
【課題を解決するための手段】以上の情況に鑑み、本発
明者らは、水に不溶性の蛋白質の高度利用を図るべく、
かかる水不溶性蛋白質から簡易かつ高収率で水溶性蛋白
質を調製するための方法について鋭意研究をすすめたと
ころ、意外にも、還元処理と酸化処理とを組み合わせ、
さらにアルカリ処理を行うことにより、水不溶性蛋白質
から容易にしかも高収率で水溶性蛋白質を製造できるこ
とを見出し、本発明を完成するに至った。SUMMARY OF THE INVENTION In view of the above circumstances, the inventors of the present invention have proposed the following in order to highly utilize water-insoluble proteins.
As a result of intensive research on a method for preparing a water-soluble protein from such a water-insoluble protein in a simple and high yield, surprisingly, a reduction treatment and an oxidation treatment were combined,
Further, it was found that a water-soluble protein can be easily produced from a water-insoluble protein in a high yield by performing an alkali treatment, and the present invention has been completed.
【０００５】即ち、本発明の要旨は、（１） 水に不溶
な蛋白質からなる原料を還元剤で処理した後に酸化剤を
含む水溶液中で処理し、続いてアルカリを添加して液性
をアルカリ性とすることを特徴とする可溶性蛋白質の製
造法、（２） 水に不溶な蛋白質からなる原料が、動物
由来の蛋白質である羽根、卵、毛、爪、角、皮、肉もし
くは魚鱗、昆虫由来の蛋白質である絹、もしくは繭、又
は植物由来の蛋白質である豆類、グルテン、米糠もしく
はその加工製品（豆腐、納豆等）である前記（１）記載
の製造法、（３） 還元剤が、硫化ナトリウム、亜硫酸
ナトリウム、重亜硫酸ナトリウム、メルカプトエタノー
ル、チオグリコール酸、アンモニウム塩、トリアルキル
ホスフィン、モノエタノールアミンサルファイド、およ
びバイサルファイドからなる群より選択されるものであ
る前記（１）又は（２）記載の製造法、（４） 還元剤
による処理温度が５０〜１００℃である前記（１）ない
し（３）いずれかに記載の製造法、（５） 酸化剤が、
過酸化水素、過酢酸、および過蟻酸からなる群より選択
されるものである前記（１）ないし（４）いずれかに記
載の製造法、（６） 酸化剤を含む水溶液が、１〜１０
％の過酸化水素水である前記（１）ないし（５）いずれ
かに記載の製造法、（７） 酸化剤を含む水溶液中での
処理温度が５０〜１００℃である前記（１）ないし
（６）いずれかに記載の製造法、（８） アルカリが、
アンモニア水、水酸化ナトリウム水溶液、炭酸ナトリウ
ム水溶液、または水酸化カリウム水溶液である前記
（１）ないし（７）いずれかに記載の製造法、（９）
アルカリ性がｐＨ８〜１１である前記（１）ないし
（８）いずれかに記載の製造法、（１０） 前記（１）
ないし（９）いずれかに記載の方法により可溶化された
蛋白質溶液を還元剤で還元処理することによるシスチン
残基含有可溶性蛋白質の製造法、並びに（１１） 前記
（１）ないし（１０）いずれかに記載の製造法によって
得られる溶解液より調製される蛋白質水溶液又は可溶性
蛋白質粉末、に関する。That is, the gist of the present invention is: (1) The raw material consisting of a protein insoluble in water is treated with a reducing agent and then treated in an aqueous solution containing an oxidizing agent, and then an alkali is added to make the liquid alkaline. A method for producing a soluble protein, characterized in that The production method according to (1) above, which is silk or cocoon which is the protein of, or beans, gluten, rice bran or a processed product thereof (tofu, natto etc.) which is a protein of plant origin, (3) The reducing agent is sulfurized Sodium, sodium sulfite, sodium bisulfite, mercaptoethanol, thioglycolic acid, ammonium salt, trialkylphosphine, monoethanolamine sulfide, and bisulfite? The production method according to (1) or (2) above, which is selected from the group consisting of: (4) the treatment temperature with a reducing agent according to any one of (1) to (3) above. Manufacturing method (5)
The method according to any one of (1) to (4) above, which is selected from the group consisting of hydrogen peroxide, peracetic acid, and formic acid, and (6) an aqueous solution containing an oxidant is 1 to 10
% Of hydrogen peroxide water, (7) The method according to any one of (1) to (5), (7) The treatment temperature in an aqueous solution containing an oxidizing agent is 50 to 100 ° C. (1) to (). 6) The production method according to any one of the items, (8) the alkali is
(9) The production method according to any one of (1) to (7) above, which is aqueous ammonia, aqueous sodium hydroxide solution, aqueous sodium carbonate solution, or aqueous potassium hydroxide solution.
The method according to any one of (1) to (8) above, wherein the alkalinity is pH 8 to 11, (10) above (1)
To (9) a method for producing a cystine residue-containing soluble protein by reducing the protein solution solubilized by the method according to any one of (1) to (10) above An aqueous protein solution or soluble protein powder prepared from the solution obtained by the production method described in 1.
【０００６】以下に本発明について詳細に説明する。本
発明に用いる蛋白質原料は水に不溶性のもので、羽根、
卵、毛髪、爪、角、皮、肉もしくは魚鱗等の動物由来の
蛋白質、大豆蛋白質、グルテン、米糠のような植物由来
の蛋白質あるいは繭や絹に代表される昆虫由来の蛋白質
等が例示されるが、これらに限定されるものではない。The present invention will be described in detail below. The protein raw material used in the present invention is insoluble in water,
Examples include animal-derived proteins such as eggs, hair, nails, horns, skins, meat or fish scales, soybean proteins, gluten, plant-derived proteins such as rice bran, and insect-derived proteins such as cocoons and silk. However, it is not limited thereto.
【０００７】本発明の可溶化処理の特徴は、不溶性蛋白
質原料をその重量の通常１０〜１００倍、好ましくは１
０〜２０倍の水中で還元処理を行い、それを水洗、及び
脱水した後に、通常１０〜１００倍、好ましくは１０〜
２０倍の水中で酸化処理を行うという２段階処理方法に
ある。The characteristic of the solubilization treatment of the present invention is that the insoluble protein raw material is usually 10 to 100 times its weight, preferably 1 time.
After reduction treatment in water of 0 to 20 times, washing and dehydrating it, it is usually 10 to 100 times, preferably 10 to 10 times.
It is a two-step treatment method in which oxidation treatment is performed in 20 times the amount of water.
【０００８】本発明に用いられる還元剤としては、無機
還元化合物、チオール類あるいはトリアルキルホスフィ
ン等が挙げられるが、具体的には、硫化ナトリウム、亜
硫酸ナトリウム、重亜硫酸ナトリウム、メルカプトエタ
ノール、チオグリコール酸、アンモニウム塩、トリアル
キルホスフィン、モノエタノールアミンサルファイド、
またはバイサルファイドが例示される。Examples of the reducing agent used in the present invention include inorganic reducing compounds, thiols, trialkylphosphines, and the like. Specifically, sodium sulfide, sodium sulfite, sodium bisulfite, mercaptoethanol, thioglycolic acid. , Ammonium salt, trialkylphosphine, monoethanolamine sulfide,
Alternatively, bisulfide is exemplified.
【０００９】還元剤の濃度は蛋白質原料に対して、通常
０．１〜１０％、好ましくは０．５〜５％程度が適当で
あり、処理温度は通常、室温〜１００℃、好ましくは７
０〜１００℃、処理時間は通常１５分〜５時間、好まし
くは３０分〜１時間程度で十分な効果が得られる。The concentration of the reducing agent is usually 0.1 to 10%, preferably 0.5 to 5%, relative to the protein raw material, and the treatment temperature is usually room temperature to 100 ° C, preferably 7
A sufficient effect can be obtained at 0 to 100 ° C. for a treatment time of usually 15 minutes to 5 hours, preferably 30 minutes to 1 hour.
【００１０】酸化剤としては、過酸化水素、過酢酸、過
蟻酸等の過酸化物が例示される。特に過酸化水素は還元
剤やカタラーゼ処理により容易に水と酸素に分解される
ので排水処理に容易である。ただし、多量の過酸化水素
の使用は製造時間の短縮をもたらすが、製造に伴う排水
の処理に手間がかかるため好ましくない。従って１〜１
０％、好ましくは３〜５％の過酸化水素水を用いる。処
理温度は通常、室温〜１００℃、好ましくは７０〜１０
０℃、処理時間は通常１〜５時間、好ましくは２〜３時
間程度で十分な効果が得られる。Examples of the oxidizing agent include peroxides such as hydrogen peroxide, peracetic acid, and formic acid. In particular, hydrogen peroxide is easily decomposed into water and oxygen by treatment with a reducing agent or catalase, so that it is easy to treat waste water. However, although the use of a large amount of hydrogen peroxide shortens the production time, it is not preferable because it takes time to treat the wastewater accompanying the production. Therefore 1 to 1
0%, preferably 3-5% hydrogen peroxide solution is used. The treatment temperature is usually room temperature to 100 ° C., preferably 70 to 10
A sufficient effect can be obtained at 0 ° C. for a treatment time of usually 1 to 5 hours, preferably 2 to 3 hours.
【００１１】不溶性の蛋白質の可溶化は、上記処理を行
った蛋白質原料液を、アンモニア水、水酸化ナトリウム
水溶液、炭酸ナトリウム水溶液、水酸化カリウム水溶液
等により通常ｐＨ７〜１１、好ましくはｐＨ８〜１１の
アルカリ性にすることにより達成される。このとき上記
の酸化処理単独では、アルカリ性にしても溶解しない活
性不溶物が混在してその分離に手間がかかり、収率も低
下するが、本発明方法では、前処理として還元処理をも
行うため、不溶物は発生せず、高収率で簡単に濾過布を
通過するような粘性のない蛋白質溶液が得られる。To solubilize insoluble proteins, the protein raw material solution treated as described above is treated with ammonia water, an aqueous sodium hydroxide solution, an aqueous sodium carbonate solution, an aqueous potassium hydroxide solution or the like to a pH of usually 7 to 11, preferably 8 to 11. This is achieved by making it alkaline. At this time, in the above-mentioned oxidation treatment alone, active insoluble matter that does not dissolve even if alkaline is mixed and it takes time to separate it, and the yield also decreases, but in the method of the present invention, reduction treatment is also performed as a pretreatment. Insoluble matter does not occur, and a protein solution having a high yield and not having a viscosity that easily passes through a filter cloth can be obtained.
【００１２】この蛋白質溶液中には未反応の酸化剤が含
まれているので、還元剤を添加することにより酸化剤を
分解することが好ましい。還元剤としては、前記の還元
処理に用いたものが使用できる。酸化剤として過酸化水
素水を用いた場合は、過酸化水素分解酵素であるカタラ
ーゼを使用することができる。還元剤を用いた場合の処
理温度は発泡を過剰に起こさせないためにも室温〜６０
℃にすることが好ましく、カタラーゼ酵素を用いる場合
は酵素の至適温度付近即ち３０〜５０℃で行うことが適
当である。Since this protein solution contains an unreacted oxidizing agent, it is preferable to decompose the oxidizing agent by adding a reducing agent. As the reducing agent, the one used in the above-mentioned reduction treatment can be used. When aqueous hydrogen peroxide is used as the oxidizing agent, catalase which is a hydrogen peroxide decomposing enzyme can be used. The treatment temperature when the reducing agent is used is room temperature to 60 to prevent excessive foaming.
C. is preferably used, and when a catalase enzyme is used, it is appropriate that the temperature be near the optimum temperature of the enzyme, that is, 30 to 50.degree.
【００１３】酸化剤の分解に使用する還元剤や酵素の使
用量は、酸化剤の濃度、処理温度、および処理時間によ
り大きく変わるため、あらかじめ確認しておく必要があ
る。例えば、酸化剤に過酸化水素水を用いた場合には、
還元剤処理後にカタラーゼを微量添加しても発泡が観察
されなければ残存過酸化水素は分解されたと見なすこと
ができる。また、このとき、使用酸化剤よりも過剰の還
元剤で処理を行った場合には、残存酸化剤の分解のみな
らず、蛋白質の還元化も起こるために酸化処理でできた
システイン酸の一部がシステインに還元される。The amount of the reducing agent or the enzyme used for decomposing the oxidant greatly depends on the concentration of the oxidant, the treatment temperature, and the treatment time, and therefore it must be confirmed in advance. For example, when hydrogen peroxide solution is used as the oxidant,
If no foaming is observed even after adding a small amount of catalase after the treatment with the reducing agent, it can be considered that the residual hydrogen peroxide has been decomposed. In addition, at this time, when treatment is performed with an excess of reducing agent than the oxidizing agent used, not only decomposition of residual oxidizing agent but also reduction of protein occurs, so part of cysteic acid formed by the oxidizing treatment occurs. Is reduced to cysteine.
【００１４】このようにして得られた蛋白質溶液は濃
縮、透析することにより塩を除き噴霧乾燥するか、ある
いはｐＨや塩濃度を調整することにより蛋白質を沈殿さ
せ、これを洗浄し凍結乾燥等することにより可溶性蛋白
質粉末を得ることができる。The protein solution thus obtained is concentrated and dialyzed to remove the salt and spray-dried, or the protein is precipitated by adjusting the pH and salt concentration, and the protein is washed and freeze-dried. Thus, a soluble protein powder can be obtained.
【００１５】本発明の方法により得られる可溶性蛋白質
は、例えば、化粧品や食品の添加物として、衣料や塗料
の改質剤として、また重金属類の捕捉剤として有用であ
る。The soluble protein obtained by the method of the present invention is useful, for example, as an additive for cosmetics and foods, as a modifier for clothing and paints, and as a scavenger for heavy metals.
【００１６】[0016]
【実施例】以下、実施例により本発明をさらに詳しく説
明するが、本発明はこれらの実施例によりなんら限定さ
れるものではない。The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.
【００１７】実施例１ ２０リットルのステンレス容器内に脱脂したアイガモの
羽根６００ｇを８０℃に調整した０．２重量％の硫化ナ
トリウム溶液１０リットルに浸した。時折攪拌しながら
３０分間還元処理を行った後、羽根を取り出し、水洗、
脱水した。次にこれを８０℃に調整した５％の過酸化水
素水１０リットル中に浸し、時折攪拌しながら２時間酸
化処理を行った。これを６０℃まで冷却し、１Ｎの炭酸
ナトリウム水溶液を加えてｐＨを９にしたところ、完全
に可溶化され、濾過布を用いてこの溶液を濾過したとこ
ろ不溶物はなく、高収率かつ容易に蛋白質溶液を調製す
ることができた。Example 1 600 g of degreased duck blades were immersed in a 20-liter stainless steel container in 10 liters of a 0.2 wt% sodium sulfide solution adjusted to 80 ° C. After performing a reduction treatment for 30 minutes with occasional stirring, take out the blades, wash with water,
Dehydrated. Next, this was immersed in 10 liters of a 5% hydrogen peroxide solution adjusted to 80 ° C. and subjected to an oxidation treatment for 2 hours with occasional stirring. This was cooled to 60 ° C, and the pH was adjusted to 9 by adding a 1N sodium carbonate aqueous solution. When the solution was completely solubilized, the solution was filtered using a filter cloth. It was possible to prepare a protein solution.
【００１８】実施例２ 実施例１で得られた蛋白質溶液１０リットルに亜硫酸ナ
トリウム５０ｇを入れて、５０℃で３０分間攪拌した。
発泡がおさまったので少量のカタラーゼ溶液を添加した
ところ、発泡が見られなかったため、完全に過酸化水素
は水と酸素に分解されたと考えられた。Example 2 To 10 liters of the protein solution obtained in Example 1 was added 50 g of sodium sulfite, and the mixture was stirred at 50 ° C. for 30 minutes.
Since bubbling subsided, a small amount of catalase solution was added, and bubbling was not observed, so it was considered that hydrogen peroxide was completely decomposed into water and oxygen.
【００１９】実施例３ 実施例２で得られた蛋白質水溶液１０リットルを１Ｎの
硫酸を用いてｐＨ４に調整したところ、白色蛋白質が析
出沈澱した。これを０．０１Ｎの硫酸溶液で洗浄し、乾
燥することにより可溶性蛋白質粉末２４０ｇ（平均分子
量１７０００）を得た。Example 3 When 10 liters of the aqueous protein solution obtained in Example 2 was adjusted to pH 4 with 1N sulfuric acid, white protein was precipitated. This was washed with a 0.01 N sulfuric acid solution and dried to obtain 240 g of soluble protein powder (average molecular weight 17,000).
【００２０】実施例４ 実施例２と同様にして得られた蛋白質水溶液１０リット
ルを透析チューブを用いて脱塩し、分子量５０００以下
の蛋白質や塩を取り除いた。続いて噴霧乾燥機で粉末化
することにより、３８０ｇの可溶性蛋白質粉末（平均分
子量１８０００）を得た。Example 4 10 liters of an aqueous protein solution obtained in the same manner as in Example 2 was desalted using a dialysis tube to remove proteins and salts having a molecular weight of 5000 or less. Then, by pulverizing with a spray dryer, 380 g of soluble protein powder (average molecular weight 18000) was obtained.
【００２１】実施例５ 実施例１で得られた蛋白質溶液から調製した蛋白質、及
び実施例３で得られた可溶性蛋白質粉末を、濃塩酸でア
ミノ酸に分解し、アミノ酸分析装置によりシスチン量を
求めたところ、実施例１で得られた蛋白質にはシスチン
はほとんど含まれていなかったのに対し、実施例３で得
られた蛋白質には２％（モル％）のシスチンが含まれて
いた。Example 5 The protein prepared from the protein solution obtained in Example 1 and the soluble protein powder obtained in Example 3 were decomposed into amino acids with concentrated hydrochloric acid, and the amount of cystine was determined by an amino acid analyzer. However, the protein obtained in Example 1 contained almost no cystine, whereas the protein obtained in Example 3 contained 2% (mol%) of cystine.
【００２２】[0022]
【発明の効果】本発明の可溶性蛋白質の製造方法によれ
ば、還元処理、及び酸化処理を組み合わせた加水分解を
行うことにより、従来の単独処理に比較して短時間で可
溶化が終了し、かつ不溶物も生じずに高収率で蛋白質水
溶液および可溶性蛋白質粉末を製造することができる。
また、大過剰の酸化剤や還元剤、重金属あるいは有機溶
媒を用いず、製造過程に残存する酸化物も分解処理する
ために、その製造廃液の処理は簡単になり、活性汚泥も
痛めない。EFFECT OF THE INVENTION According to the method for producing a soluble protein of the present invention, solubilization is completed in a shorter time as compared with the conventional single treatment by performing hydrolysis in which reduction treatment and oxidation treatment are combined, In addition, an aqueous protein solution and a soluble protein powder can be produced in high yield without producing insoluble matter.
Further, since a large excess of oxidizing agent, reducing agent, heavy metal or organic solvent is not used, and the oxide remaining in the production process is decomposed, the production waste liquid is easily treated and activated sludge is not damaged.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ０７Ｋ 14/465 8318−4Ｈ 14/47 8318−4Ｈ ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI Technical indication C07K 14/465 8318-4H 14/47 8318-4H
Claims (11)
で処理した後に酸化剤を含む水溶液中で処理し、続いて
アルカリを添加して液性をアルカリ性とすることを特徴
とする可溶性蛋白質の製造法。1. A soluble protein characterized by treating a raw material of a protein insoluble in water with a reducing agent and then treating it in an aqueous solution containing an oxidizing agent, and subsequently adding an alkali to make the liquid alkaline. Manufacturing method.
由来の蛋白質である羽根、卵、毛、爪、角、皮、肉もし
くは魚鱗、昆虫由来の蛋白質である絹、もしくは繭、又
は植物由来の蛋白質である豆類、グルテン、米糠もしく
はその加工製品（豆腐、納豆等）である請求項１記載の
製造法。2. A raw material consisting of a protein insoluble in water is animal-derived protein such as feather, egg, hair, nail, horn, skin, meat or fish scale, insect-derived protein silk or cocoon, or plant. The method according to claim 1, which is a protein derived from beans, gluten, rice bran or processed products thereof (tofu, natto, etc.).
リウム、重亜硫酸ナトリウム、メルカプトエタノール、
チオグリコール酸、アンモニウム塩、トリアルキルホス
フィン、モノエタノールアミンサルファイド、およびバ
イサルファイドからなる群より選択されるものである請
求項１又は請求項２記載の製造法。3. The reducing agent is sodium sulfide, sodium sulfite, sodium bisulfite, mercaptoethanol,
The method according to claim 1 or 2, which is selected from the group consisting of thioglycolic acid, ammonium salt, trialkylphosphine, monoethanolamine sulfide, and bisulfide.
である請求項１ないし請求項３いずれか１項に記載の製
造法。4. The treatment temperature with the reducing agent is 50 to 100 ° C.
The manufacturing method according to any one of claims 1 to 3.
過蟻酸からなる群より選択されるものである請求項１な
いし請求項４いずれか１項に記載の製造法。5. The method according to claim 1, wherein the oxidizing agent is selected from the group consisting of hydrogen peroxide, peracetic acid, and formic acid.
酸化水素水である請求項１ないし請求項５いずれか１項
に記載の製造法。6. The method according to claim 1, wherein the aqueous solution containing an oxidizing agent is 1 to 10% hydrogen peroxide solution.
０〜１００℃である請求項１ないし請求項６いずれか１
項に記載の製造法。7. The treatment temperature in an aqueous solution containing an oxidizing agent is 5
It is 0 to 100 ° C., and any one of claims 1 to 6.
The production method described in the section.
リウム水溶液、炭酸ナトリウム水溶液、または水酸化カ
リウム水溶液である請求項１ないし請求項７いずれか１
項に記載の製造法。8. The alkali according to claim 1, which is aqueous ammonia, sodium hydroxide aqueous solution, sodium carbonate aqueous solution, or potassium hydroxide aqueous solution.
The production method described in the section.
１ないし請求項８いずれか１項に記載の製造法。9. The production method according to claim 1, wherein the alkaline property has a pH of 8 to 11.
に記載の方法により可溶化された蛋白質溶液を還元剤で
還元処理することによるシスチン残基含有可溶性蛋白質
の製造法。10. A method for producing a cystine residue-containing soluble protein by reducing the protein solution solubilized by the method according to any one of claims 1 to 9 with a reducing agent.
項に記載の製造法によって得られる溶解液より調製され
る蛋白質水溶液又は可溶性蛋白質粉末。11. The method according to any one of claims 1 to 10.
An aqueous protein solution or a soluble protein powder prepared from the solution obtained by the production method according to the item.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33162994A JPH08165298A (en) | 1994-12-09 | 1994-12-09 | Production of water soluble protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP33162994A JPH08165298A (en) | 1994-12-09 | 1994-12-09 | Production of water soluble protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08165298A true JPH08165298A (en) | 1996-06-25 |
Family
ID=18245799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP33162994A Pending JPH08165298A (en) | 1994-12-09 | 1994-12-09 | Production of water soluble protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08165298A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030000884A (en) * | 2001-06-27 | 2003-01-06 | 주식회사 밀텍 | Method of Crude Protein with Livestock Waste Matter |
| CN101921572A (en) * | 2010-08-10 | 2010-12-22 | 刘雷 | Method for making amino albumin powder capable of capturing formaldehyde |
| CN102392353A (en) * | 2011-07-22 | 2012-03-28 | 福建众和股份有限公司 | Finishing technology of chicken feather regenerated protein fabric printed and dyed cloth |
| US20190021391A1 (en) * | 2017-07-20 | 2019-01-24 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
| US11523623B2 (en) | 2019-01-18 | 2022-12-13 | R.J. Reynolds Tobacco Company | Plant-derived protein purification |
| US11659844B1 (en) | 2016-08-12 | 2023-05-30 | Zee Company I, Llc | System for increasing antimicrobial efficacy in a poultry processing tank |
| US11839858B1 (en) | 2016-02-17 | 2023-12-12 | Zee Company I, Llc | Peracetic acid concentration and monitoring and concentration-based dosing system |
-
1994
- 1994-12-09 JP JP33162994A patent/JPH08165298A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030000884A (en) * | 2001-06-27 | 2003-01-06 | 주식회사 밀텍 | Method of Crude Protein with Livestock Waste Matter |
| CN101921572A (en) * | 2010-08-10 | 2010-12-22 | 刘雷 | Method for making amino albumin powder capable of capturing formaldehyde |
| CN102392353A (en) * | 2011-07-22 | 2012-03-28 | 福建众和股份有限公司 | Finishing technology of chicken feather regenerated protein fabric printed and dyed cloth |
| US11839858B1 (en) | 2016-02-17 | 2023-12-12 | Zee Company I, Llc | Peracetic acid concentration and monitoring and concentration-based dosing system |
| US11659844B1 (en) | 2016-08-12 | 2023-05-30 | Zee Company I, Llc | System for increasing antimicrobial efficacy in a poultry processing tank |
| US11968988B1 (en) | 2016-08-12 | 2024-04-30 | Zeco, Llc | System for increasing antimicrobial efficacy in a poultry processing tank |
| US20190021391A1 (en) * | 2017-07-20 | 2019-01-24 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
| US10757964B2 (en) | 2017-07-20 | 2020-09-01 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
| US10834959B2 (en) * | 2017-07-20 | 2020-11-17 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
| US11805805B2 (en) | 2017-07-20 | 2023-11-07 | R.J. Reynolds Tobacco Company | Purification of tobacco-derived protein compositions |
| US11523623B2 (en) | 2019-01-18 | 2022-12-13 | R.J. Reynolds Tobacco Company | Plant-derived protein purification |
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