JPH08131198A - Evaluation of preventing effect on putrefaction of sludge by atp method - Google Patents
Evaluation of preventing effect on putrefaction of sludge by atp methodInfo
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- JPH08131198A JPH08131198A JP27869194A JP27869194A JPH08131198A JP H08131198 A JPH08131198 A JP H08131198A JP 27869194 A JP27869194 A JP 27869194A JP 27869194 A JP27869194 A JP 27869194A JP H08131198 A JPH08131198 A JP H08131198A
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- sludge
- atp
- ozone
- evaluating
- sulfate
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はＡＴＰ法を利用してオゾ
ン処理による汚泥の腐敗防止効果を評価する方法に関す
るものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for evaluating the effect of preventing ozone sludge from spoilage by utilizing the ATP method.
【０００２】[0002]
【従来の技術】近年の下水道の普及に伴い、下水処理水
量は年々増加しており、汚泥の発生量もほぼ同じ比率
（約５％）の割合で増加している。このような汚泥の処
理は、処分地に制約の大きい大都市のみならず、処理を
新たに開始した中小都市の場合も大きな問題となってい
る。2. Description of the Related Art With the spread of sewerage in recent years, the amount of treated sewage has been increasing year by year, and the amount of sludge generated has been increasing at the same rate (about 5%). Such sludge treatment is a big problem not only in large cities with large restrictions on the disposal site, but also in small and medium-sized cities that have newly started treatment.
【０００３】この汚泥処理の解決策の一つとして、汚泥
の集約処理が注目されている。この汚泥の集約処理の長
所は（１）汚泥処理施設のスケールメリット、（２）汚
泥処理場の集約化による環境対策、（３）エネルギー回
収の効率化、（４）維持管理面でのコストの低減化、
（５）汚泥の資源化の向上等が挙げられる。As one of the solutions for sludge treatment, attention is being paid to sludge intensive treatment. The advantages of this sludge intensive treatment are (1) economies of scale of sludge treatment facilities, (2) environmental measures by consolidating sludge treatment plants, (3) efficient energy recovery, and (4) cost reduction in maintenance management. Reduction,
(5) Improving the recycling of sludge.
【０００４】上記汚泥の集約処理では、下水処理場と汚
泥集約処理施設間で汚泥を長距離輸送する必要がある。
この時に汚泥輸送管内で汚泥の腐敗現象が生じやすいと
いう問題がある。汚泥の腐敗は主に嫌気性微生物が汚泥
中の有機物を分解することにより起こる現象である。In the sludge aggregating treatment, it is necessary to transport the sludge for a long distance between the sewage treatment plant and the sludge aggregating treatment facility.
At this time, there is a problem that the sludge decay phenomenon easily occurs in the sludge transportation pipe. Sludge decay is a phenomenon mainly caused by anaerobic microorganisms decomposing organic matter in sludge.
【０００５】このような汚泥の腐敗によって以下の問題
点が発生する。即ち、 （イ）汚泥処理施設の腐食 硫酸塩還元菌の存在により汚泥中に硫化水素が生成し、
これにより輸送管内に生息する硫黄酸化細菌が気相に放
出された硫化水素を硫酸に酸化して管路施設を腐食す
る。The following problems occur due to the decay of such sludge. That is, (a) Corrosion of sludge treatment facility Hydrogen sulfide is generated in the sludge due to the presence of sulfate reducing bacteria,
As a result, the sulfur-oxidizing bacteria inhabiting the transportation pipe oxidize the hydrogen sulfide released in the gas phase into sulfuric acid and corrode the pipeline facility.
【０００６】（ロ）悪臭の発生 上記の硫化水素は悪臭物質であるため、近隣住民に迷惑
を与える外、硫化水素濃度が１００ｐｐｍ以上になると
猛毒化して現場の作業員が非常な危険にさらされる。(B) Generation of offensive odor Since the above-mentioned hydrogen sulfide is a foul-smelling substance, in addition to causing annoyance to neighboring residents, when the hydrogen sulfide concentration becomes 100 ppm or more, it becomes extremely poisonous and workers at the site are exposed to great danger. .
【０００７】（ハ）濃縮性の悪化 腐敗により汚泥濃度が低下すると、汚泥と水との比重差
が小さくなり、重力による濃縮が困難になる。(C) Deterioration of Concentration Property When the sludge concentration decreases due to decay, the difference in specific gravity between the sludge and water becomes small, and concentration by gravity becomes difficult.
【０００８】（ニ）脱水性の悪化 脱水性が悪化すると凝集に必要な凝集剤の添加量が増大
し、ランニングコストが増加する。(D) Deterioration of dehydration property When the dehydration property is deteriorated, the addition amount of the coagulant necessary for coagulation increases and the running cost increases.
【０００９】（ホ）返流水の負荷増大 汚泥の腐敗により汚泥中の有機物量が増大すると、水処
理系への循環負荷量も増大し、処理水質の悪化を招来す
る。(E) Increase in the load of return water When the amount of organic matter in sludge increases due to the decay of sludge, the amount of circulation load on the water treatment system also increases, and the quality of treated water deteriorates.
【００１０】一方、微生物のエネルギー代謝の中心はＡ
ＴＰ−ＡＤＰ系である。ＡＤＰ（アデノシン２リン酸）
は代謝過程で生じる高エネルギー化合物からリン酸基を
受取ってＡＴＰ（アデノシン３リン酸）になる。このＡ
ＴＰがいろいろな合成反応に利用され、運動、分泌、吸
収等の生理活性のエネルギー源となる。ＡＴＰは生きた
微生物にとって必要不可欠なものであり、従って該ＡＴ
Ｐは微生物活性の指標として用いることができる。On the other hand, the center of microbial energy metabolism is A
It is a TP-ADP system. ADP (adenosine diphosphate)
Receives a phosphate group from a high-energy compound generated in the metabolic process and becomes ATP (adenosine triphosphate). This A
TP is used in various synthetic reactions and serves as an energy source for physiological activity such as exercise, secretion and absorption. ATP is essential for living microorganisms and therefore the AT
P can be used as an index of microbial activity.
【００１１】[0011]
【発明が解決しようとする課題】汚泥を長距離輸送する
際の汚泥腐敗問題で施設の腐食とか悪臭の発生の主な原
因は硫化水素である。この硫化水素は下式のように硫酸
塩還元菌により硫酸塩が還元して発生する。Hydrogen sulfide is the main cause of facility corrosion and the generation of foul odors due to sludge spoilage problems during long-distance transportation of sludge. This hydrogen sulfide is generated by the reduction of sulfate by sulfate reducing bacteria as shown in the following formula.
【００１２】 ＳＯ4 2-＋２Ｃ＋２Ｈ2Ｏ → ２ＨＣＯ3 -＋Ｈ2Ｓ ・・・・・・・（１） そこで汚泥の腐敗を防止するために、本出願人は汚泥と
オゾンガスを接触させ、硫酸塩還元菌等の嫌気性細菌を
死滅させる方法を前回の出願で提案したが、この時のオ
ゾンによる汚泥の腐敗防止効果を評価する方法として、
（Ａ）硫酸塩還元菌を計数する方法、（Ｂ）オゾン処理
した汚泥を三角フラスコに投入後、その汚泥を２５℃の
嫌気条件下で放置し、発生した硫化水素ガス（Ｈ
2Ｓ）、溶存硫化物（Ｓ2-）、硫酸イオン（ＳＯ4 2-）を
測定する方法等が挙げられる。SO 4 2− + 2C + 2H 2 O → 2HCO 3 − + H 2 S (1) In order to prevent the sludge from spoiling, the applicant of the present invention brings the sludge into contact with ozone gas to form a sulfate salt. We proposed a method to kill anaerobic bacteria such as reducing bacteria in the previous application, but as a method to evaluate the spoilage spoilage prevention effect by ozone at this time,
(A) Method for counting sulfate-reducing bacteria, (B) Ozone-treated sludge is put into an Erlenmeyer flask, and the sludge is left under anaerobic conditions at 25 ° C. to generate hydrogen sulfide gas (H
2 S), dissolved sulfide (S 2− ), sulfate ion (SO 4 2− ) and the like.
【００１３】しかしながら（Ａ）の硫酸塩還元菌を計数
する方法は嫌気性の培養装置が必要である上、結果を得
るまでに７日から１０日も要してしまうという難点があ
り、（Ｂ）のオゾン処理した汚泥を２５℃の嫌気条件下
で放置して発生した硫化水素ガス（Ｈ2Ｓ）を測定する
方法は、密閉状態でガスを捕集する必要がある上、結果
を得るまでに１２時間以上を要するという難点がある。
更に同（Ｂ）のオゾン処理した汚泥を２５℃の嫌気条件
下で放置して発生した溶存硫化物（Ｓ2-）又は硫酸イオ
ン（ＳＯ4 2-）を測定する方法は、分析に高価なイオン
クロマトグラフィーを必要とする上、結果を得るまでに
２４時間以上を要するという問題点が存在する。However, the method (A) for counting sulfate-reducing bacteria requires an anaerobic culturing apparatus and has a drawback that it takes 7 to 10 days to obtain a result. The method for measuring the hydrogen sulfide gas (H 2 S) generated by leaving the ozone-treated sludge under anaerobic conditions at 25 ° C. is required to collect the gas in a sealed state, and until the result is obtained. The problem is that it takes 12 hours or more.
Furthermore, the method of measuring dissolved sulfide (S 2− ) or sulfate ion (SO 4 2− ) generated by leaving the ozone-treated sludge of the same (B) under anaerobic conditions at 25 ° C. is expensive for analysis. In addition to requiring ion chromatography, there is a problem that it takes more than 24 hours to obtain a result.
【００１４】特に季節や天候により濃度及び性状が異な
る汚泥が下水処理場から発生することが予想され、その
対策として汚泥腐敗防止に必要なオゾン注入率も大きく
変化する可能性がある。しかしオゾンによる汚泥の腐敗
防止効果を評価するため、上記したように結果を得るま
でに７〜１０日要する硫酸塩還元菌計数法を実施しても
意味がない。なぜなら汚泥腐敗防止に必要なオゾン注入
率の決定に長時間を要してしまうので、この硫酸塩還元
菌の計数結果をオゾン注入率に反映させることができな
いからである。[0014] In particular, it is expected that sludge having different concentrations and properties depending on the season and weather will be generated from the sewage treatment plant, and as a countermeasure against this, the ozone injection rate necessary for preventing sludge decay may change greatly. However, in order to evaluate the effect of ozone to prevent sludge from spoiling, it is meaningless to carry out the sulfate-reducing bacteria counting method, which takes 7 to 10 days to obtain the results as described above. This is because it takes a long time to determine the ozone injection rate necessary for preventing sludge decay, and the count result of the sulfate-reducing bacteria cannot be reflected in the ozone injection rate.
【００１５】そこで本発明はこのようなオゾンによる汚
泥の腐敗防止効果を評価する方法が有している各種の問
題点を解消して、迅速且つ簡便な操作で短時間に評価す
る方法を提供することを目的とするものである。Therefore, the present invention solves various problems of the method for evaluating the effect of preventing sludge from decaying by ozone, and provides a method for evaluating in a short time by a quick and simple operation. That is the purpose.
【００１６】[0016]
【課題を解決するための手段】本発明は上記の目的を達
成するために、オゾン処理を行った汚泥を採取して試料
を作成し、この試料のＡＴＰを抽出してから、該抽出液
に発光試薬を加えて発光量を測定し、この発光強度から
オゾン注入率に基づく汚泥の腐敗防止作用を評価するよ
うにしたＡＴＰ法による汚泥腐敗防止効果の評価方法を
提供する。In order to achieve the above-mentioned object, the present invention collects ozone-treated sludge to prepare a sample, extracts ATP of the sample, and then uses the extracted solution. Provided is a method for evaluating the effect of preventing sludge decay by the ATP method, which comprises adding a luminescent reagent to measure the amount of luminescence and evaluating the effect of preventing rot of sludge from the luminescence intensity based on the ozone injection rate.
【００１７】前記ＡＴＰ抽出用試薬としてトリクロロ酢
酸を用いる。又、ＡＴＰの発光試薬としてルシフェリ
ン，ルシフェラーゼを用いている。Trichloroacetic acid is used as the ATP extraction reagent. Also, luciferin and luciferase are used as luminescent reagents of ATP.
【００１８】[0018]
【作用】かかるＡＴＰ法による汚泥腐敗防止効果の評価
方法によれば、サンプリングした汚泥にトリクロロ酢酸
を加えて撹拌することによりＡＴＰが抽出される。そし
てトリス緩衝液を加えて希釈してからルシフェールの発
光試薬とＡＴＰ抽出液を加えて発光量を測定することに
よってオゾン注入率とＡＴＰの相関関係が得られるの
で、この関係からオゾン注入率に基づく汚泥の腐敗防止
作用を評価することが可能となり、合わせて最適なオゾ
ン注入率を決定することができる。According to such a method for evaluating the sludge decay prevention effect by the ATP method, ATP is extracted by adding trichloroacetic acid to the sampled sludge and stirring. Then, by adding Tris buffer and diluting it, and then adding the lucifer luminescent reagent and ATP extract to measure the amount of luminescence, the correlation between ozone injection rate and ATP can be obtained. It becomes possible to evaluate the anti-corruption effect of sludge, and the optimum ozone injection rate can be determined together.
【００１９】特に本実施例は従来の硫酸塩還元菌を計数
する方法等ときわめて良好な相関関係があり、しかも従
来法よりも簡易な手段であるＡＴＰ法を用いて汚泥腐敗
防止効果の評価方法が確立される。In particular, this example has a very good correlation with the conventional method for counting sulfate-reducing bacteria, etc., and is a method of evaluating the sludge decay prevention effect using the ATP method which is a simpler means than the conventional method. Is established.
【００２０】[0020]
【実施例】以下本発明にかかるＡＴＰ法による汚泥腐敗
防止効果の評価方法の具体的な実施例を説明する。図１
は実験に使用した半回分式のオゾン処理装置であり、１
はオゾン接触槽であって、このオゾン接触槽１は恒温槽
２中に浸漬されている。このオゾン接触槽１には撹拌機
構３と測定機器４が付設されていて、この測定機器４に
よって水温，ｐＨ，溶存オゾン濃度が計測される。恒温
槽２には温度調節器５が付設されている。EXAMPLE A specific example of the method for evaluating the sludge decay prevention effect by the ATP method according to the present invention will be described below. FIG.
Is a semi-batch type ozone treatment device used in the experiment.
Is an ozone contact tank, and this ozone contact tank 1 is immersed in a constant temperature tank 2. The ozone contact tank 1 is provided with a stirring mechanism 3 and a measuring device 4, and the measuring device 4 measures water temperature, pH, and dissolved ozone concentration. A temperature controller 5 is attached to the constant temperature bath 2.
【００２１】６は酸素発生器付きのオゾン発生機であ
り、このオゾン発生機６から導出された管路７が流量計
８を介してオゾン接触槽１内に導入され、該オゾン接触
槽１の底壁近傍に配置された散気管９に連結されてい
る。１０は排オゾン処理装置、１１は制御部であり、こ
の制御部１１はオゾン濃度計の計測値に基づいてオゾン
接触槽１に対する注入オゾン濃度の制御と、オゾン接触
槽１から排オゾン処理装置１０に送り込む排オゾン制御
を行う。１２は排オゾン用の流量計である。Reference numeral 6 denotes an ozone generator equipped with an oxygen generator, and a conduit 7 led out of the ozone generator 6 is introduced into the ozone contact tank 1 through a flow meter 8 and the ozone contact tank 1 It is connected to an air diffuser 9 arranged near the bottom wall. Reference numeral 10 is an exhaust ozone treatment device, 11 is a control unit, and this control unit 11 controls the injection ozone concentration into the ozone contact tank 1 based on the measurement value of the ozone concentration meter, and the exhaust ozone treatment device 10 from the ozone contact tank 1 Exhaust ozone control to be sent to. 12 is a flow meter for exhaust ozone.
【００２２】オゾン接触槽１は有効容積５０Ｌ（直径２
６１ｍｍ，高さ１３００ｍｍの管状体）とした。実験は
先ず該オゾン接触槽１内に５０Ｌの初沈汚泥を入れ、オ
ゾン発生機６から得られる濃度５０（ｇ／Ｎｍ3）のオ
ゾンガスを流量（２Ｌ／分）を維持して散気管９から放
散し、排オゾンガスは上壁部から排オゾン処理装置１０
に導いて処理した。オゾン処理中のオゾン接触槽１は恒
温槽２の温度調節器５によって２５℃に保持し、オゾン
ガスの放散中は撹拌機構３を駆動して汚泥の撹拌を行っ
た。The ozone contact tank 1 has an effective volume of 50 L (diameter 2
The tubular body was 61 mm in height and 1300 mm in height. In the experiment, first, 50 L of the first sludge was placed in the ozone contact tank 1, and the ozone gas having a concentration of 50 (g / Nm 3 ) obtained from the ozone generator 6 was maintained from the diffuser pipe 9 while maintaining the flow rate (2 L / min). The emitted ozone gas is exhausted from the upper wall of the exhaust ozone treatment device 10
It was led to and processed. The ozone contact tank 1 during the ozone treatment was kept at 25 ° C. by the temperature controller 5 of the constant temperature tank 2, and the stirring mechanism 3 was driven to stir the sludge while the ozone gas was being diffused.
【００２３】そしてサンプリング時間（分）として、
０，１２.５，２５，５０，７５，１００，１５０，２
００分を設定して各時間毎にオゾン処理中の汚泥のサン
プリングを行った。Then, as the sampling time (minutes),
0, 12.5, 25, 50, 75, 100, 150, 2
The sludge during the ozone treatment was sampled every hour with 00 minutes set.
【００２４】サンプリングした汚泥は以下に記す操作手
段によりＡＴＰを抽出してから、該抽出液に発光試薬を
加えて発光量を測定した。尚、本実施例の測定原理であ
るＡＴＰ（アデノシン三リン酸）法について簡単に説明
すると、一般にＡＴＰは微生物活性を表わす指標として
用いられており、ＡＴＰがルシフェリン，ルシフェラー
ゼと反応して発光する原理を利用している。このＡＴＰ
の抽出法はいくつか知られているが、一般には試料を試
験管に取り、トリス緩衝液を加えて沸騰水浴中で撹拌し
ながら抽出を行い、得られた検水を蛍光光度計のキュベ
ットに入れて蛍光光度を測定する方法を用いる。測定原
理は下式の通りである。ATP was extracted from the sampled sludge by the operation means described below, and a luminescent reagent was added to the extracted solution to measure the amount of luminescence. The ATP (adenosine triphosphate) method, which is the measurement principle of the present example, will be briefly described. Generally, ATP is used as an index showing microbial activity, and the principle that ATP reacts with luciferin and luciferase to emit light Are using. This ATP
Although several extraction methods are known, in general, take a sample in a test tube, add Tris buffer and extract while stirring in a boiling water bath, and put the obtained test water in a cuvette of a fluorometer. A method of measuring fluorescence intensity is used. The measurement principle is as follows.
【００２５】 〔実施例〕 （１）試験管に上記サンプリングした汚泥を１（ｍｌ）
とトリクロロ酢酸を１（ｍｌ）加え、３０秒間ボルテッ
クスで撹拌してＡＴＰを抽出する。そしてトリス緩衝液
を１０ｍｌ加えて希釈する。この時点でＡＴＰは１２倍
に希釈されたことになる。[0025] [Example] (1) 1 (ml) of the sampled sludge in a test tube
And 1 (ml) of trichloroacetic acid are added, and ATP is extracted by vortexing for 30 seconds. Then, 10 ml of Tris buffer is added to dilute. At this point, ATP had been diluted 12 times.
【００２６】（２）汚泥活性度測定装置（キッコーマン
製ＵＰＤ−２０００ＥＸ）用の試薬キット「ルシフェー
ル−ＡＳ」の発光試薬０.２５ｍｌと上記ＡＴＰ抽出液
をポリエチレンチューブに加え、発光量をルミノメータ
（明電舎製ＵＰＤ−８０００）で測定する。(2) 0.25 ml of the luminescent reagent of the reagent kit "Lucifer-AS" for the sludge activity measuring device (UPD-2000EX manufactured by Kikkoman) and the above ATP extract solution were added to a polyethylene tube, and the luminescence amount was measured by a luminometer (Meidensha). (Made by UPD-8000).
【００２７】図２は測定により得られたオゾン注入率
（ｍｇ／Ｌ）とＡＴＰ（ｍｏｌ／ｌ）の相関図であり、
同図からオゾン注入率が１００（ｍｇ／Ｌ）近辺からＡ
ＴＰが急激に減少し、オゾン注入率が約２００（ｍｇ／
Ｌ）でＡＴＰが４.２×１０-11まで減少した。その後は
ほぼ一定の値となった。FIG. 2 is a correlation diagram of the ozone injection rate (mg / L) and ATP (mol / l) obtained by the measurement,
From the figure, the ozone injection rate is around 100 (mg / L) and A
TP decreased sharply and ozone injection rate was about 200 (mg /
In A), ATP decreased to 4.2 × 10 -11 . After that, it became almost constant.
【００２８】次に比較例としてオゾンによる汚泥の腐敗
防止効果、つまり硫酸塩還元菌の消長を培養計数法で求
めた。Next, as a comparative example, the effect of ozone for preventing spoilage from spoilage, that is, the fate of sulfate-reducing bacteria was determined by a culture counting method.
【００２９】〔比較例〕 （１）前記サンプリングした汚泥を、１，０.１，０.０
１・・・・・・・（ｍｌ）含むように減菌水を用いて希釈し、
各希釈段階での希釈試料の１ｍｌをシャーレに取る。こ
れに４５℃に保った硫酸塩還元菌用培地を２０ｍｌ加
え、均一に混合する。出来上がった寒天平板培地を嫌気
性培養装置に入れ、３０℃で培養する。Comparative Example (1) 1,0.1,0.0
Dilute with sterile water to contain 1 ...
Take 1 ml of the diluted sample at each dilution step into a Petri dish. 20 ml of a medium for sulfate-reducing bacteria kept at 45 ° C. is added to this and mixed uniformly. The resulting agar plate medium is placed in an anaerobic culture device and cultured at 30 ° C.
【００３０】（２）硫酸塩還元菌が存在する場合には、
２〜３日で黒色集落が形成される。これは硫酸塩が乳酸
ナトリウムにより還元され、直ちに鉄と反応して黒色の
硫化鉄が生成されるためである。そこで７〜１０日後の
黒色の集落を計数し、４，７，１０日で最も多いものを
採用する。(2) When sulfate reducing bacteria are present,
A black settlement is formed in a few days. This is because the sulfate is reduced by sodium lactate and immediately reacts with iron to produce black iron sulfide. Therefore, black colonies after 7 to 10 days are counted, and the one with the highest number is selected from 4, 7, and 10 days.
【００３１】図３は比較例の結果から得られたオゾン注
入率（ｍｇ／Ｌ）と汚泥中の硫酸塩還元菌数（ｃｅｌｌ
ｓ／ｍｌ）の相関図であり、同図からオゾン処理前の硫
酸塩還元菌数は１.４×１０-6（個／ｍｌ）であった。
この数値は汚泥中に含まれる硫酸塩還元菌数とほぼ同じ
レベルであった。オゾン処理で硫酸塩還元菌は最初ほと
んど減少しなかったが、前記ＡＴＰの例と同様にオゾン
注入率が５０（ｍｇ／Ｌ）近辺から硫酸塩還元菌数が急
激に減少し、オゾン注入率が約２００（ｍｇ／Ｌ）で２
桁まで減少した。FIG. 3 shows the ozone injection rate (mg / L) and the number of sulfate-reducing bacteria in the sludge (cell) obtained from the results of the comparative example.
s / ml), and the number of sulfate-reducing bacteria before ozone treatment was 1.4 × 10 −6 (cells / ml).
This number was almost the same level as the number of sulfate-reducing bacteria contained in sludge. Although the amount of sulfate-reducing bacteria was scarcely reduced at first by the ozone treatment, the number of sulfate-reducing bacteria was drastically decreased from around 50 (mg / L) as in the case of the ATP, and the ozone injection ratio was 2 at about 200 (mg / L)
It has decreased to the digit.
【００３２】上記のように本実施例にかかる方法、即
ち、サンプリングされた試料のＡＴＰを抽出し、該抽出
液に発光試薬を加えて発光量を測定する方法と、汚泥中
の硫酸塩還元菌の消長を培養計数法で求める方法とが良
好な相関関係を保っており、従って簡易な手段であるＡ
ＴＰ法によって汚泥腐敗防止効果の評価方法を確立でき
ることが判明した。As described above, the method according to the present embodiment, that is, the method of extracting ATP of the sampled sample, adding the luminescent reagent to the extracted solution and measuring the luminescence amount, and the sulfate reducing bacteria in sludge Has a good correlation with the method of determining the fate of A by the culture counting method, and is therefore a simple means.
It was found that the TP method can establish an evaluation method for the sludge decay prevention effect.
【００３３】[0033]
【発明の効果】以上詳細に説明したように、本発明にか
かるＡＴＰ法による汚泥腐敗防止効果の評価方法によれ
ば、オゾン処理装置からサンプリングした汚泥にトリク
ロロ酢酸を加えてＡＴＰを抽出してから緩衝液を加えて
希釈し、ルシフェール発光試薬とＡＴＰ抽出液を加えて
発光量を測定することにより、オゾン注入率とＡＴＰの
相関関係が容易に得られるので、この関係からオゾン注
入率に基づく汚泥の腐敗防止作用を評価するとともに最
適なオゾン注入率を決定することができる。従って季節
や天候により濃度及び性状が異なる汚泥が下水処理場か
ら発生した場合であっても、汚泥腐敗防止に必要なオゾ
ン量の変化に素早く対応してすることができて、直ちに
オゾン注入率を変化させる等の対策を取ることができ
る。As described above in detail, according to the method for evaluating the sludge decay prevention effect by the ATP method according to the present invention, trichloroacetic acid is added to the sludge sampled from the ozone treatment apparatus to extract ATP. Correlation between ozone injection rate and ATP can be easily obtained by adding and diluting buffer solution and adding lucifer luminescent reagent and ATP extract solution to measure luminescence amount. From this relationship, sludge based on ozone injection rate is obtained. The optimum ozone injection rate can be determined while evaluating the anti-corrosion effect of Therefore, even when sludge with different concentration and properties depending on the season and weather is generated from the sewage treatment plant, it is possible to quickly respond to changes in the amount of ozone necessary to prevent sludge decay, and immediately adjust the ozone injection rate. Measures such as changing can be taken.
【００３４】特に本実施例の場合には、汚泥へのオゾン
注入率を変化させた時のＡＴＰと硫酸塩還元菌の消長は
ほぼ同じ傾向があり、特に０.５〜３％の汚泥濃度が異
なる汚泥を用いてＡＴＰ法と硫酸塩還元菌法で評価を実
施したところ、ＡＴＰと硫酸塩還元菌の消長はほぼ同じ
傾向を示すことが確認された。しかも従来法よりも簡易
な手段であって３０分以内に結果を得ることができるＡ
ＴＰ法を用いて汚泥腐敗防止に必要な汚泥へのオゾン注
入率を効率的に決定することができる。In particular, in the case of this example, the rates of ATP and sulfate-reducing bacteria tended to be almost the same when the ozone injection rate into the sludge was changed, and especially when the sludge concentration was 0.5 to 3%. When the ATP method and the sulfate-reducing bacteria method were evaluated using different sludges, it was confirmed that the profiles of ATP and the sulfate-reducing bacteria showed almost the same tendency. Moreover, the method is simpler than the conventional method and the result can be obtained within 30 minutes.
The TP method can be used to efficiently determine the ozone injection rate into sludge, which is necessary to prevent sludge decay.
【００３５】更に実施に際し、迅速且つ簡便な操作で短
時間に測定を行うことができるとともに、高価な設備を
不要としてコストの面からも有利であり、格別の精度と
か熟練した測定技術を必要としないという効果が得られ
る。Further, in carrying out the measurement, it is possible to perform the measurement in a short time by a quick and simple operation, and it is advantageous from the viewpoint of cost because expensive equipment is not required, and special accuracy or skilled measurement technology is required. The effect of not doing so is obtained.
【図１】本実施例で使用した半回分式のオゾン処理装置
例を示す概要図。FIG. 1 is a schematic diagram showing an example of a semi-batch type ozone treatment device used in this embodiment.
【図２】本実施例に基づくオゾン注入率とＡＴＰの相関
関係を示すグラフ。FIG. 2 is a graph showing the correlation between the ozone injection rate and ATP based on this example.
【図３】比較例に基づくオゾン注入率と汚泥中の硫酸塩
還元菌数の相関関係を示すグラフ。FIG. 3 is a graph showing the correlation between the ozone injection rate and the number of sulfate-reducing bacteria in sludge based on Comparative Example.
１…オゾン接触槽 ２…恒温槽 ３…撹拌機構 ４…測定機器 ５…温度調節器 ６…オゾン発生機 ８，１２…流量計 ９…散気管 １０…排オゾン処理装置 １１…制御部 DESCRIPTION OF SYMBOLS 1 ... Ozone contact tank 2 ... Constant temperature tank 3 ... Stirring mechanism 4 ... Measuring device 5 ... Temperature controller 6 ... Ozone generator 8, 12 ... Flowmeter 9 ... Air diffuser 10 ... Exhaust ozone treatment device 11 ... Control unit
Claims (3)
を作成し、この試料のＡＴＰを抽出してから、該抽出液
に発光試薬を加えて発光量を測定し、この発光強度から
オゾン注入率に基づく汚泥の腐敗防止作用を評価するこ
とを特徴とするＡＴＰ法による汚泥腐敗防止効果の評価
方法。1. An ozone-treated sludge is sampled to prepare a sample, ATP of the sample is extracted, and then a luminescent reagent is added to the extract to measure the luminescence amount. A method for evaluating a sludge decay prevention effect by the ATP method, which comprises evaluating a sludge decay prevention action based on an injection rate.
酢酸を用いた請求項１記載のＡＴＰ法による汚泥腐敗防
止効果の評価方法。2. The method for evaluating the sludge decay prevention effect by the ATP method according to claim 1, wherein trichloroacetic acid is used as the ATP extraction reagent.
ン，ルシフェラーゼを用いた請求項１，２記載のＡＴＰ
法による汚泥腐敗防止効果の評価方法。3. The ATP according to claim 1, wherein luciferin and luciferase are used as a luminescent reagent for the ATP.
Evaluation method of sludge decay prevention effect by method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27869194A JPH08131198A (en) | 1994-11-14 | 1994-11-14 | Evaluation of preventing effect on putrefaction of sludge by atp method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27869194A JPH08131198A (en) | 1994-11-14 | 1994-11-14 | Evaluation of preventing effect on putrefaction of sludge by atp method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08131198A true JPH08131198A (en) | 1996-05-28 |
Family
ID=17600835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27869194A Pending JPH08131198A (en) | 1994-11-14 | 1994-11-14 | Evaluation of preventing effect on putrefaction of sludge by atp method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08131198A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8189213B2 (en) | 2006-03-15 | 2012-05-29 | Konica Minolta Business Technologies, Inc. | Image forming apparatus capable of using security code for communication |
| JP2019508019A (en) * | 2015-12-22 | 2019-03-28 | オムヤ インターナショナル アーゲー | Microbial cell viability assay for detection or determination of slurry contamination |
-
1994
- 1994-11-14 JP JP27869194A patent/JPH08131198A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8189213B2 (en) | 2006-03-15 | 2012-05-29 | Konica Minolta Business Technologies, Inc. | Image forming apparatus capable of using security code for communication |
| JP2019508019A (en) * | 2015-12-22 | 2019-03-28 | オムヤ インターナショナル アーゲー | Microbial cell viability assay for detection or determination of slurry contamination |
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