JPH0755793A - Test piece for analysis - Google Patents

Abstract

PURPOSE:To simply detect a colored ingredient by so holding fibrous structures between two water impermeable support plates by aligning ends that one end is used as a liquid sample suction port, providing an opening at the one plate, and measuring a coloring degree of the structure. CONSTITUTION:This test piece comprises a thin fibrous structure 1 held between two water impermeable support plates 2 by substantially aligning ends of the structure 1 and the plates 2 as to suck liquid sample from a thick part of one end of the structure 1 to form a sample suction port 3. In order to directly measure a colored state of the structure 1 due to suction of the sample by a reflectivity change, etc., a measuring opening 4 is provided on an upper surface of the one structure 1 of the plate 2. In the case of measuring by this test piece, a sample amount to be sucked by capillarity is always constant, and a quantitative measuring operation using a pipet, etc., is eliminated.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、ワイン、果汁、イン
ク、血液や尿等の体液等の液体試料に含まれる有色成分
を簡便，迅速に検出し得る分析用試験片に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a test piece for analysis which can easily and quickly detect a colored component contained in a liquid sample such as wine, fruit juice, ink, body fluid such as blood or urine.

【０００２】[0002]

【従来の技術】医療診断，環境計測，発酵モニター，食
品の生産，販売などの品質管理に於いて、液体試料中の
被分析物濃度の測定は重要である。これらの測定は、従
来、液体試料の一部を取り出し、分光光度計を用い、そ
の試料の吸光度より、被分析物濃度を求めるという手法
が広く行われてきた。しかし、染色業に於ける色の調配
合，食品の製造、販売に於ける品質管理、開業医に於け
る血清，血漿中のビリルビン濃度分析などにおいては、
装置が大型かつ高価であり、また、測定に必要とされる
試料量が比較的多量で、取扱いが複雑であった。従っ
て、このような分光光度計に替わる、より簡便，迅速か
つ安価に液体試料中の被分析物濃度を測定できる装置
（分析用試験片）の開発が望まれていた。2. Description of the Related Art Measurement of the concentration of an analyte in a liquid sample is important in quality control such as medical diagnosis, environmental measurement, fermentation monitoring, food production and sales. For these measurements, a method has conventionally been widely used in which a part of a liquid sample is taken out and a spectrophotometer is used to determine the analyte concentration from the absorbance of the sample. However, in color adjustment in the dyeing industry, quality control in food production and sales, serum and plasma bilirubin concentration analysis in practitioners, etc.
The device was large and expensive, and the amount of sample required for the measurement was relatively large, and the handling was complicated. Therefore, it has been desired to develop an apparatus (analytical test piece) which can replace the spectrophotometer and can measure the concentration of the analyte in the liquid sample more simply, quickly and inexpensively.

【０００３】[0003]

【発明が解決しようとする課題】そこで、本発明者ら
は、こうした現状に鑑み鋭意研究の結果、本発明に至っ
たもので、本発明の目的は、ピペット等の特別な用具を
用いて行う定量計量操作を必要とすることなく、簡便、
迅速、安価かつ高精度に、液体試料中の被分析物濃度を
測定できる分析用試験片を提供することにある。Therefore, the present inventors have accomplished the present invention as a result of earnest research in view of the present situation, and an object of the present invention is to carry out using a special tool such as a pipette. Simple and easy without the need for quantitative weighing operation
An object is to provide an analytical test piece capable of quickly, inexpensively and highly accurately measuring the concentration of an analyte in a liquid sample.

【０００４】[0004]

【課題を解決するための手段】上述の目的は、液体試料
中の成分を分析するための試験片であって、薄片状の繊
維質構造体を、その一端が液体試料吸い込み口となるよ
う、２枚の水不浸透性支持板間に三者端部が略揃うよう
にして挾持し、更に、該水不浸透性支持板の一方に、繊
維質構造体の着色程度を計測するための測定用開口部を
設けてなることを特徴とする分析用試験片により達成さ
れる。The above-mentioned object is a test piece for analyzing components in a liquid sample, wherein a thin fibrous structure is used, one end of which serves as a liquid sample suction port. A measurement for holding the two water-impermeable support plates so that their three ends are substantially aligned with each other, and further measuring the degree of coloring of the fibrous structure on one of the water-impermeable support plates. It is achieved by an analytical test piece characterized by being provided with an opening for use.

【０００５】以下、本発明を詳述する。本発明の分析用
試験片の一実施態様例を図１に示すが、本発明はこの例
により何ら制限されるものではない。同図において、１
は繊維質構造体、２は水不浸透性支持板、３は試料吸い
込み口、４は測定用開口部である。The present invention will be described in detail below. One embodiment of the analytical test piece of the present invention is shown in FIG. 1, but the present invention is not limited to this example. In the figure, 1
Is a fibrous structure, 2 is a water-impermeable support plate, 3 is a sample suction port, and 4 is a measurement opening.

【０００６】図１に示す分析用試験片は、２枚の水不浸
透性支持板２で、薄片状の繊維質構造体１を挟持してお
り、その際、繊維質構造体１一端の厚み部分から液体試
料を吸い込むことができるように、繊維質構造体１端部
と水不浸透性支持板２端部とを略揃うようにし、試料吸
い込み口３を形成してなる。また、水不浸透性支持板２
の一方の繊維質構造体１上面に、液体試料吸収による繊
維質構造体の着色状況を直接反射率変化等で計測するた
めの測定用開口部４を設けてなる。The analytical test piece shown in FIG. 1 has two thin water-impervious support plates 2 sandwiching a thin fibrous structure 1. At this time, the thickness of one end of the fibrous structure 1 is large. The sample suction port 3 is formed so that the end of the fibrous structure 1 and the end of the water-impermeable support plate 2 are substantially aligned so that the liquid sample can be sucked from the portion. In addition, the water-impermeable support plate 2
On the upper surface of one of the fibrous structures 1, there is provided a measurement opening 4 for directly measuring the coloring state of the fibrous structure due to absorption of the liquid sample by a change in reflectance or the like.

【０００７】上記試料吸い込み口３は、図１に示すよう
に、繊維質構造体１一端の厚み部分またはその近傍に設
けると、試料吸い込みの際の操作が容易となるので好適
である。また、試料吸い込み口３に於いて、２枚の支持
板２の端部と繊維質構造体１の端部とは、完全に一致し
ていてもよいし、一致していなくても良い。すなわち、
液体試料の粘度、使用可能な試料量、用いる繊維質構造
体の目付け密度の違い等により、液体試料の吸い込み状
況が異なるため、適宜、必要に応じて支持板の端部より
繊維質構造体をわずかに突出させたり、引っ込めたりす
ればよい。また、上下の支持板２の端部を完全に揃えな
くてもよい。また、試料吸い込み口３に対向する繊維質
構造体１の側端部は、開放されている方が吸い込み速度
がより早くなるので好ましい。このように、試験片に吸
い込み口３を設けることにより、従来の点着式の試験片
とは異なり、試料に試験片を接触させることによって試
料を吸収することができるので、腹部、上腕部等を穿刺
して得られる極少量の血液等の測定も可能となる。As shown in FIG. 1, it is preferable that the sample suction port 3 is provided in the thickness portion of one end of the fibrous structure 1 or in the vicinity thereof because the operation for sucking the sample becomes easy. In the sample suction port 3, the ends of the two support plates 2 and the ends of the fibrous structure 1 may or may not be completely aligned. That is,
The suction condition of the liquid sample differs depending on the viscosity of the liquid sample, the amount of sample that can be used, the weight density of the fibrous structure used, and so on.If necessary, place the fibrous structure from the end of the support plate as necessary. It may be slightly projected or retracted. Moreover, the upper and lower support plates 2 do not have to be perfectly aligned at their ends. Further, the side end of the fibrous structure 1 facing the sample suction port 3 is preferably opened because the suction speed becomes higher. As described above, by providing the suction port 3 in the test piece, unlike the conventional spotting type test piece, the sample can be absorbed by bringing the test piece into contact with the sample, so that the abdomen, upper arm, etc. It is also possible to measure an extremely small amount of blood or the like obtained by puncturing.

【０００８】また、測定用開口部４の位置は、液体試料
吸い込み口３から試料を吸い込むときに、開口部４が直
接試料に触れないように、開口部４の端部が試料吸い込
み口３より０．５ｍｍ以上、好ましくは１ｍｍ以上離れ
ていることが好適である。なお、この距離をあまり長く
すると、吸い込んだ試料が開口部に達するまでに時間を
要したり、試料必要量が多くなったりするので、微量試
料、高粘度試料への適用性の点で、１０ｍｍ以内にして
おくことが好ましい。また、測定用開口部４の形状は、
任意に構成すればよく、また、その大きさは、光計測時
の位置合わせの容易さ、光計測に於ける計測誤差の低減
により、形状を円形とした場合、直径３〜６ｍｍが適当
である。また、本発明に於いては、開口部４の裏面は、
繊維質構造体１が支持体２により固定されているので、
液体試料の吸収により繊維質構造体１が変形することが
なく好適である。The position of the measurement opening 4 is such that the end of the opening 4 is located above the sample suction port 3 so that the opening 4 does not come into direct contact with the sample when sucking the sample from the liquid sample suction port 3. It is suitable that they are separated by 0.5 mm or more, preferably 1 mm or more. If this distance is set too long, it will take time for the sucked sample to reach the opening, and the required amount of sample will increase. It is preferable to keep it within the range. The shape of the measurement opening 4 is
It may be configured arbitrarily, and its size is preferably 3 to 6 mm in diameter when the shape is circular because of the ease of alignment during light measurement and the reduction of measurement error in light measurement. . Further, in the present invention, the back surface of the opening 4 is
Since the fibrous structure 1 is fixed by the support 2,
It is preferable that the fibrous structure 1 is not deformed by the absorption of the liquid sample.

【０００９】本発明で用いる水不浸透性支持板２として
は、ポリエステル、ポリエチレン、ポリプロピレン、ナ
イロン、テフロン、ポリ塩化ビニル等が挙げられ、中で
も、強度、成形性等の点から、ポリエステル、ポリ塩化
ビニルが好ましい。また、水不浸透性支持板の厚さは、
試験片を構成した際の取扱いの容易さから８０〜３００
μｍが好ましい。また、水不浸透性支持板２の形状は、
図１に示すような形状の他、長方形等としてもよいが、
図１に示すような形状とすると試料の吸い込みが容易と
なるので好適である。また、水不浸透性支持板は、繊維
質構造体より大きくし、試料吸い込み口及びその対向端
部以外は繊維質構造体を被覆するようにすることが試料
吸収量を一定とすることができ好適である。Examples of the water-impermeable support plate 2 used in the present invention include polyester, polyethylene, polypropylene, nylon, Teflon, polyvinyl chloride and the like. Among them, from the viewpoint of strength, moldability and the like, polyester and polychlorinated Vinyl is preferred. In addition, the thickness of the water-impermeable support plate,
80 to 300 because of the ease of handling when the test piece is constructed
μm is preferred. The shape of the water-impermeable support plate 2 is
Besides the shape shown in FIG. 1, it may be rectangular or the like,
The shape as shown in FIG. 1 is preferable because the sample can be easily sucked in. Also, the water impermeable support plate should be larger than the fibrous structure, and the fibrous structure should be covered except for the sample inlet and its opposite end so that the sample absorption amount can be kept constant. It is suitable.

【００１０】また、繊維質構造体１の大きさは、小さす
ぎると光反射率計測における測定誤差が大きくなった
り、取扱いが困難になるため、幅２〜６ｍｍ、長さ５〜
２０ｍｍの薄片状とすることが適当である。更に、繊維
質構造体１の厚さは１００〜４００μが好ましく、厚さ
バラツキのないものがよい。また、その形状は、板状で
も、円板状でもよい。If the size of the fibrous structure 1 is too small, the measurement error in the light reflectance measurement becomes large and the handling becomes difficult. Therefore, the width is 2 to 6 mm and the length is 5 to 5.
It is suitable to have a flaky shape of 20 mm. Furthermore, the thickness of the fibrous structure 1 is preferably 100 to 400 μ, and it is preferable that the fibrous structure 1 has no thickness variation. Further, the shape thereof may be a plate shape or a disk shape.

【００１１】上記繊維質構造体１としては、織物，編
物，不織布，ロ紙等が挙げられ、中でも、繊維質構造体
自体の均一性が良好で発色時の色斑が起こりにくい織編
物が高精度測定には適する。更に、編物を用いること
が、繊維質構造体を必要な大きさに裁断する際にほつれ
にくいこと、光を照射した際の反射光の均一性が高いこ
と、液体を吸い込んだ際に於ける液の拡散速度が、拡散
方向によらず一定なこと等により好ましい。編物組織に
ついては、トリコット編み、平編み、丸編み、平打ち等
が挙げられる。Examples of the fibrous structure 1 include woven fabrics, knitted fabrics, non-woven fabrics, papers, and the like. Among them, woven and knitted fabrics having high uniformity of the fibrous structure itself and less likely to cause color spots during color development Suitable for precision measurement. Furthermore, the use of a knitted fabric makes it difficult to fray when cutting a fibrous structure into a required size, has high uniformity of reflected light when irradiated with light, and has a liquid content when sucking a liquid. It is preferable that the diffusion rate is constant regardless of the diffusion direction. Examples of the knit structure include tricot knitting, flat knitting, circular knitting, and flat knitting.

【００１２】また、繊維質構造体１を構成する繊維素材
としては、毛，綿，麻，絹等の天然繊維、ナイロン６，
ナイロン６６等のポリアミド，ポリエチレンテレフタレ
ート，ポリブチレンテレフタレート，その共重合体等の
ポリエステル，アクリル，ビニロン，アセテート，レー
ヨン等の合成繊維が挙げられる。これらは、単独でまた
は２種以上併用し複合繊維としたもの、また、混紡した
ものが用いられる。The fiber material constituting the fibrous structure 1 includes natural fibers such as wool, cotton, linen, silk, nylon 6, and the like.
Examples thereof include polyamides such as nylon 66, polyethylene terephthalate, polybutylene terephthalate, polyesters such as copolymers thereof, and synthetic fibers such as acrylic, vinylon, acetate and rayon. These may be used alone or in combination of two or more to form a composite fiber, or may be a blended fiber.

【００１３】天然繊維において、綿は短繊維であるため
毛羽が発生することがあり、絹等は細繊度であるが、湿
潤時の膨潤による平面性の低下が問題となることがあ
る。従って、製造の容易性、湿潤時に於ける繊維質構造
体の低膨潤性の点から、ポリエステル、ポリアミド等の
合成繊維フィラメントが好ましい。Among natural fibers, cotton is a short fiber and thus may have fluff, and silk and the like have a fineness, but there is a problem that the flatness is deteriorated due to swelling when wet. Therefore, synthetic fiber filaments such as polyester and polyamide are preferable from the viewpoint of easy production and low swelling of the fibrous structure when wet.

【００１４】また、本発明に用いられる繊維の太さは、
単糸繊度５デニール以下の繊維を用いると、織物，編物
等を構成した際に繊維質構造体が４００μ以下の厚さに
なり、光を照射した際に均一な反射光が得られ好適であ
る。更に、単糸繊度３デニール以下のものを用いると、
液体試料を吸い込む際の毛細管現象が生じやすく、かつ
構造体内の表面積を大きくし、保持し得る試薬量を多く
することができ、更に、試薬と被分析物との接触場とし
て広い面積を確保できるので好適である。The thickness of the fiber used in the present invention is
When fibers having a single yarn fineness of 5 denier or less are used, the fibrous structure has a thickness of 400 μ or less when a woven fabric, a knitted fabric or the like is formed, and uniform reflected light can be obtained when irradiated with light, which is preferable. . Furthermore, if a single yarn fineness of 3 denier or less is used,
Capillary phenomenon is likely to occur when sucking a liquid sample, the surface area inside the structure can be increased, and the amount of reagent that can be held can be increased, and a large area can be secured as a contact field between the reagent and the analyte. Therefore, it is preferable.

【００１５】更に、単糸繊度１デニール以下の繊維は、
その表面積が、例えば、１デニールの異形断面糸（例え
ば偏平率１０の偏平糸）で６０００ｃｍ² ／ｇ、０．１
デニールの丸断面糸で９６００ｃｍ² ／ｇと格段に広い
表面積を有する。このような広表面積繊維は、同一面積
内に存在する構成繊維本数を増やさずとも、繊維の総表
面積を大きくすることができる。このため、十分な毛細
管現象を得ることができると共に表面の平坦性を得るこ
とができ特に好ましい。更に、検出用試薬類を繊維質表
面に多量に吸着保持するためにも好適である。以上のこ
とから、単糸繊度１デニール以下の合成繊維が好適であ
る。Further, for fibers having a single yarn fineness of 1 denier or less,
The surface area is, for example, 6000 cm ^{2 /} g, 0.1 with a modified cross-section yarn of 1 denier (for example, a flat yarn with an aspect ratio of 10).
Denier round cross-section yarn has a remarkably large surface area of 9600 cm ² / g. Such a large surface area fiber can increase the total surface area of the fiber without increasing the number of constituent fibers existing in the same area. Therefore, it is particularly preferable because sufficient capillarity can be obtained and the flatness of the surface can be obtained. Further, it is also suitable for adsorbing and holding a large amount of the detection reagents on the fiber surface. From the above, synthetic fibers having a single yarn fineness of 1 denier or less are preferable.

【００１６】また、単糸繊度１デニール以下の繊維は、
直接目的の細繊度糸を紡糸して得られたもの、物性の異
なる２種のポリマーよりなる複合繊維を溶解や分割等の
手段により細繊度化されたものが挙げられる。中でも、
製造容易性、後述の布帛密度、血液等の液体試料に対す
るポリマーの親和性、試料と接触した場合に於ける繊維
質構造体の低膨潤性等からみて、ポリアミド及びポリエ
ステルよりなる複合繊維を編物となした後フィブリル化
して得られたものが好ましい。Fibers having a single yarn fineness of 1 denier or less are
Examples thereof include those obtained by directly spinning the desired fine fiber, and those obtained by finely arranging the composite fiber composed of two kinds of polymers having different physical properties by means such as dissolution or division. Above all,
From the viewpoint of ease of production, cloth density described later, affinity of polymer for liquid sample such as blood, low swelling property of fibrous structure when contacted with sample, etc., composite fiber composed of polyamide and polyester is used as knitted fabric. Those obtained by fibrillating after the treatment are preferable.

【００１７】フィブリル化法としては、特公昭５３−３
５６３３号公報等に記載された公知の方法等が用い得、
中でも、ベンジルアルコールを用いる方法が好ましい。
この工程の一例を示すと、ベンジルアルコールをパディ
ング法等で織編物に付与して複合繊維を開繊せしめ、次
に、水洗、乾燥、ヒートセットする方法が挙げられる。
かかる工程において、ベンジルアルコールによる処理温
度は２０〜３０℃が好ましい。また、必要に応じて、適
宜開繊工程に続き飽和水蒸気等による収縮工程を実施し
てもよい。As a fibrillation method, Japanese Patent Publication No. 53-3
Known methods described in Japanese Patent No. 5633 can be used,
Among them, the method using benzyl alcohol is preferable.
An example of this step is a method in which benzyl alcohol is applied to the woven or knitted material by a padding method or the like to open the composite fiber, and then the composite fiber is washed with water, dried, and heat set.
In this step, the treatment temperature with benzyl alcohol is preferably 20 to 30 ° C. Further, if necessary, a shrinking step using saturated steam or the like may be appropriately performed following the opening step.

【００１８】このフィブリル化型複合繊維としては、種
々のものが公知であり、本発明には、ポリアミド成分と
ポリエステル成分とが単一フィラメントの任意の横断面
において、一方の成分を他方の成分が完全に包囲するこ
となく両成分が接合された形状を有する複合繊維であ
り、具体的には、横断面がサイドバイサイド型の複合繊
維（図２（ｄ））、サイドバイサイド繰り返し型の複合
繊維（図２（ｅ））、放射型の形状を有する部分と該放
射部を補完する形状を有する他の成分からなる複合繊維
（図２（ａ），（ｂ））、該形状に中空部を設けた繊維
（図２（ｃ））等が好ましい。Various kinds of fibrillated composite fibers are known, and in the present invention, one component of the polyamide component and the polyester component are combined with the other component in an arbitrary cross section of a single filament. A composite fiber having a shape in which both components are joined together without being completely surrounded, and specifically, a side-by-side type composite fiber (FIG. 2D) and a side-by-side repeating type composite fiber (FIG. 2). (E)), a composite fiber composed of a portion having a radial shape and another component having a shape that complements the radiation portion (FIGS. 2A and 2B), a fiber having a hollow portion in the shape (FIG. 2 (c)) and the like are preferable.

【００１９】中でも、放射型の形状を有する部分と該放
射部を補完するくさび型の形状を有する他の成分からな
る複合繊維（図２（ａ），（ｂ））が好ましく、放射型
の形状を有する部分としてポリアミド、くさび型の形状
を有する部分としてポリエステルを用い、このセグメン
トの繊度が０．２デニール以下のものが好ましい。Among them, a composite fiber (FIGS. 2 (a) and 2 (b)) consisting of a portion having a radial shape and another component having a wedge shape that complements the radiation portion is preferable, and the radial shape is preferable. It is preferable that polyamide is used as the portion having a groove and polyester is used as the portion having a wedge shape, and the fineness of this segment is 0.2 denier or less.

【００２０】また、上記繊維を編物とする場合、編目密
度は、コース（縦目）×ウエル（横目）の値が３０００
から６０００が好ましい。６０００を超える場合、密度
が高すぎるため粘度の高い試料等は浸透速度が遅くなる
傾向にあり、逆に、３０００未満の場合、試料によって
は測定範囲が狭くなったり、測定バラツキが大きくなっ
たりする傾向にある。また、コースとウエルはほぼそろ
えた方が、反射率の均一性に優れるため好ましい。When the above fibers are knitted, the stitch density is 3000 (course (longitudinal) × well (horizontal)).
To 6000 are preferred. If it exceeds 6000, the sample having high viscosity tends to have a slow permeation rate because the density is too high. On the contrary, if it is less than 3000, the measurement range becomes narrow or the measurement variation becomes large depending on the sample. There is a tendency. Further, it is preferable that the course and the well are substantially aligned because the uniformity of the reflectance is excellent.

【００２１】上記繊維質構造体１と支持板２とは、繊維
質構造体１が支持板２間に単に挟み込まれて保持された
状態でも、両面粘着テープ等の粘着剤、接着剤等で両者
が密着している状態でも良い。なお、このとき、接着剤
成分が、繊維質構造体組織の内部まで拡散し、測定に影
響したり、試料の拡散を抑制したりすることがないよう
にする。The fibrous structure 1 and the support plate 2 are bonded to each other with an adhesive such as a double-sided adhesive tape or an adhesive even when the fibrous structure 1 is simply sandwiched between the support plates 2 and held. May be in close contact. At this time, the adhesive component is prevented from diffusing into the inside of the tissue of the fibrous structure, affecting the measurement, and suppressing the diffusion of the sample.

【００２２】繊維質構造体が単に挟み込まれた場合、液
体試料の吸い込み速度は速くなるが、試料に試験片を接
触する時間が試料吸い込み量に影響することがある。一
方、繊維質構造体が支持板と密着している場合は、最低
必要な試料への接触時間（試料吸い込み開始後試料が開
口部位まで展開し、更に試験片内の微小空間が試料で満
たされるまでの時間）経過後、試験片を引き続き試料に
接触していても試料吸い上げ量が増加せずに測定値の変
動が生じないため高精度の測定に適する。また、繊維質
構造体が試料との接触により膨潤しやすいような場合に
は、繊維質構造体が支持板に密着している方が測定面の
平面性が崩れにくく好ましい結果を与える。また、繊維
質構造体は、支持板の開口部周辺部分に於いて支持板に
密着していることが好ましい。When the fibrous structure is simply sandwiched, the suction speed of the liquid sample increases, but the time of contacting the test piece with the sample may affect the suction amount of the sample. On the other hand, when the fibrous structure is in close contact with the support plate, the minimum required contact time with the sample (the sample develops to the open area after the start of sample suction, and the microspace inside the test piece is filled with the sample). After the lapse of time), even if the test piece is continuously in contact with the sample, the sample suction amount does not increase and the measured value does not fluctuate, which is suitable for highly accurate measurement. Further, when the fibrous structure is likely to swell due to contact with the sample, it is preferable that the fibrous structure is in close contact with the support plate because the flatness of the measurement surface is less likely to collapse. Moreover, it is preferable that the fibrous structure is in close contact with the support plate in the peripheral portion of the opening of the support plate.

【００２３】また、試料吸い込み口３周辺部は、図３
、に示すように繊維質構造体が支持板と密着してい
ても、密着していなくても実用上差し支えはないが、血
液のように比較的粘度の高い試料を測定する際には、吸
い込み口周辺は密着していない方が試料の吸い込み開始
がスムーズである。これは両面粘着テープがスペーサー
のような役目をし、繊維質構造体と支持板間に生じた間
隙に試料が先ず毛細管現象で入るため、繊維質構造体と
試料との接触面積が増加し、繊維質構造体内への試料の
拡散がスムーズに生じるためと考えられる。The periphery of the sample suction port 3 is shown in FIG.
As shown in, the fibrous structure may or may not be in close contact with the support plate, but it does not matter in practice, but when measuring a sample with a relatively high viscosity such as blood, suction If the area around the mouth is not in close contact, it is easier to start sucking the sample. This is because the double-sided adhesive tape acts as a spacer and the sample first enters the gap created between the fibrous structure and the support plate by the capillary phenomenon, so that the contact area between the fibrous structure and the sample increases, It is considered that this is because the sample diffuses smoothly into the fibrous structure.

【００２４】また、試料溶液の吸い上げをより円滑に
し、かつ反射率測定を高感度に行うために、必要に応じ
て、繊維質構造体に各種試薬を保持させてもよい。例え
ば、反射光測定に於いて入射光の光反射剤として機能す
る光反射剤微粉末を配合してもよい。光遮蔽剤微粉末と
しては、白色度が高い方が好ましく、具体的には、酸化
チタン、合成シリカ、炭酸マグネシウム、ケイ酸アルミ
ニウム等が挙げられ、中でも、酸化チタンが特に好適で
ある。また、光遮蔽剤微粉末は、発色ムラを起こすこと
がなく、繊維質構造体内への拡散を抑制できるよう粒径
０．３〜１．０μｍのものを用いることが好ましい。If necessary, various reagents may be held in the fibrous structure in order to more smoothly suck up the sample solution and to measure the reflectance with high sensitivity. For example, fine powder of a light-reflecting agent that functions as a light-reflecting agent for incident light in reflected light measurement may be blended. The light-shielding agent fine powder preferably has high whiteness, and specific examples thereof include titanium oxide, synthetic silica, magnesium carbonate, and aluminum silicate. Among them, titanium oxide is particularly preferable. In addition, it is preferable to use the light shielding agent fine powder having a particle size of 0.3 to 1.0 μm so as to prevent uneven coloring and suppress diffusion into the fibrous structure.

【００２５】その他、必要に応じて、少量の界面活性
剤、例えば、非イオン性界面活性剤、陰イオン性界面活
性剤、陽イオン性界面活性剤、両性イオン性界面活性剤
等を配合してもよい。この界面活性剤は繊維構造体の水
ぬれ性を向上させ、試料の円滑な吸い込みに有効に作用
する。界面活性剤は製造時の試薬含有溶液中、０．０１
〜０．１重量％の量で添加する事が好ましい。更に、本
発明の試験片には、吸い込んだ試料を反射率測定まで安
定に保持するために、ｐＨ緩衝液剤を加えてもよい。In addition, if necessary, a small amount of a surface active agent such as a nonionic surface active agent, an anionic surface active agent, a cationic surface active agent, and a zwitterionic surface active agent may be added. Good. This surfactant improves the wettability of the fibrous structure with water and effectively acts on the smooth suction of the sample. The surfactant is 0.01% in the reagent-containing solution at the time of production.
It is preferably added in an amount of 0.1 wt%. Furthermore, a pH buffer solution may be added to the test piece of the present invention in order to hold the sucked sample stably until the reflectance measurement.

【００２６】本発明の分析用試験片を製造する一例を示
すと、まず、必要に応じて光反射剤粉末、緩衝塩、界面
活性剤等を溶媒に溶解または分散させる。このなかに適
当な大きさの繊維質構造体を浸漬する。このとき、液が
内部まで確実に入り込むようにするため減圧処理を施す
ことが好ましい。その後、繊維質構造体を所定の大きさ
に裁断し、図１に示すように水不浸透性支持板に挟み込
む。なお、水不浸透性支持板は、その片面に両面接着テ
ープを貼り付けた後に適宜必要な大きさに裁断し、更
に、支持板の一方には、測定用開口部を設けるようにす
ると、試験片の製造を容易に行うことができる。An example of producing the analytical test piece of the present invention will be described. First, if necessary, a light reflecting agent powder, a buffer salt, a surfactant and the like are dissolved or dispersed in a solvent. A fibrous structure having an appropriate size is immersed in this. At this time, it is preferable to carry out a decompression process so that the liquid can surely enter the inside. After that, the fibrous structure is cut into a predetermined size and sandwiched between the water-impermeable support plates as shown in FIG. In addition, the water-impermeable support plate is cut to a required size after attaching a double-sided adhesive tape on one side thereof, and further, one of the support plates is provided with a measurement opening, and the test is conducted. The pieces can be easily manufactured.

【００２７】次に、被分析物の濃度測定は、例えば、分
光光度計を用いた吸光光度法がランバート−ベールの法
則に基づき、被分析物濃度を特定波長の吸光度値より算
出するのと同様に、反射率分光分析法に関して導かれた
クベルカ−ムンクの式により、反射率測定から求められ
たＫ／Ｓ値と、被分析物濃度との関係より試料中の被分
析物量を定量する。Next, the concentration of the analyte is measured, for example, by the absorptiometric method using a spectrophotometer based on the Lambert-Beer law, and the concentration of the analyte is calculated from the absorbance value at a specific wavelength. In addition, the amount of the analyte in the sample is quantified from the relationship between the K / S value obtained from the reflectance measurement and the analyte concentration by the Kubelka-Munk equation derived for the reflectance spectroscopy.

【００２８】下記式（１）は、前述のクベルカ−ムンク
式を簡略化したものである。 Ｋ／Ｓ＝（１−Ｒｔ）² ／２Ｒｔ （１） （但し、Ｒｔは、特定の時間ｔで測定された反射率であ
り、式（２）により定義される量である。 Ｒｔ＝（ｒｔ−ｒｂ）／（ｒｏ−ｒｂ） （２） （但し、ｒｂは試験片からの反射光がない場合の光強度
測定値、ｒｔは時間ｔに於ける光強度測定値及びｒｏは
試験片に白色板を置いた場合の光強度測定値であ
る。））The following equation (1) is a simplified version of the Kubelka-Munk equation described above. K / S = (1−Rt) ² / 2Rt (1) (where Rt is the reflectance measured at a specific time t and is an amount defined by the equation (2). Rt = (rt -Rb) / (ro-rb) (2) (where, rb is the light intensity measurement value when there is no reflected light from the test piece, rt is the light intensity measurement value at time t, and ro is the white color on the test piece. It is the light intensity measurement value when the plate is placed.))

【００２９】吸光光度法に於ける測定に於いては、入射
光が試料溶液に入射する角度，試料溶液中を通過する光
路長等が変化すると、検出器に到達する光量が変化する
ため、濃度と吸光度の一定の関係が維持できなくなり濃
度の正確な算出ができない。このため、分光光度計で
は、濃度と吸光度との関係が、不用意に変化することが
ないようにされている。これと同様に反射率測定による
定量に於いても、入射光角度，反射材表面の形状等の測
定条件を一定に保ち、反射材表面に於ける入射光の散
乱，反射，吸収量の変動が、Ｋ／Ｓ値と被分析物濃度の
関係を乱さぬように留意しなければならない。本発明の
試験片は、反射率測定に於けるこれらの事項にも充分留
意して達成されたものである。また、要求される定量の
精度によっては分析用試験片の着色とカラーチャートと
を目視により比較して濃度を算出してもよい。In the measurement by the absorptiometric method, when the incident angle of the incident light on the sample solution, the optical path length passing through the sample solution, etc. are changed, the amount of light reaching the detector is changed. The constant relationship between the absorbance and the absorbance cannot be maintained and the concentration cannot be accurately calculated. Therefore, in the spectrophotometer, the relationship between the concentration and the absorbance is prevented from changing carelessly. Similarly, in the quantitative measurement by the reflectance measurement, the measurement conditions such as the incident light angle and the shape of the surface of the reflecting material are kept constant, and the scattering, reflection and absorption amount of the incident light on the surface of the reflecting material are changed. , K / S values and the analyte concentration should not be disturbed. The test piece of the present invention was achieved by paying close attention to these items in reflectance measurement. Further, depending on the required accuracy of quantitative determination, the concentration may be calculated by visually comparing the coloring of the analytical test piece with the color chart.

【００３０】本発明の分析用試験片を用いた測定の一例
について述べる。すなわち、まず、測定すべき液体試料
に本試験片の試料吸い込み口３を接触させる。すると、
液体試料は、繊維質構造体１自体及び繊維質構造体１と
水不浸透性支持板２間に形成される隙間に毛細管現象に
より吸い込まれていく。An example of measurement using the analytical test piece of the present invention will be described. That is, first, the sample suction port 3 of the test piece is brought into contact with the liquid sample to be measured. Then,
The liquid sample is sucked into the gap formed between the fibrous structure 1 itself and the water impermeable support plate 2 by the capillary phenomenon.

【００３１】この際、測定に供される液体試料は、染
料、インク、食品製造用タンク内の多量の果汁、腹部，
上腕部等を専用の穿刺針で穿刺して得られるような極微
量の血液等の有色成分を含有するものである。試料採取
はそれら液体試料に試験片の吸い込み口３を接触させる
だけで良く、試料の吸い込みに要する時間も極めて短く
て良い。例えば、繊維質構造体を１デニール以下のナイ
ロンとポリエステルのフィラメントよりなる編物とし、
編物の厚さを２００μｍ、幅５ｍｍに裁断したものを用
いて、光反射率計測用の開口部の端を吸い込み口から２
ｍｍのところに設けた場合、この開口部位内の編物（測
光部）まで、試料が拡散してくるために必要な試料量
は、３〜５μｌと極微量でよい。At this time, the liquid sample used for the measurement is dye, ink, a large amount of fruit juice in a food manufacturing tank, an abdomen,
It contains an extremely small amount of a colored component such as blood obtained by puncturing the upper arm or the like with a dedicated puncture needle. Sampling can be performed simply by bringing the suction port 3 of the test piece into contact with the liquid sample, and the time required for sucking the sample can be extremely short. For example, the fibrous structure is a knitted fabric composed of nylon and polyester filaments of 1 denier or less,
Using a knitted product cut to a thickness of 200 μm and a width of 5 mm, the end of the opening for measuring the light reflectance is cut from the suction port to 2
When it is provided at a position of mm, the sample amount necessary for the sample to diffuse up to the knitted fabric (photometric portion) in the opening portion may be an extremely small amount of 3 to 5 μl.

【００３２】また、試料が開口部位に完全に到達するま
でに要する時間は、液体試料の粘度，繊維質構造体の性
質にもよるが、インク等の粘度の余り高くないものを試
料にした場合、１秒程度であり、液体試料の吸い込みが
迅速に行われる。The time required for the sample to completely reach the opening depends on the viscosity of the liquid sample and the properties of the fibrous structure, but when a sample such as ink having a very low viscosity is used as the sample. It takes about 1 second, and the liquid sample is quickly sucked.

【００３３】従って、試験片を液体試料に接触させるに
必要な最低時間は、使用する試料、試験片作製時の諸条
件により異なるが、５秒以内でよく、インク等の粘度の
余り高くないものを試料にした場合には１秒程度でよ
い。尚、試料に接触させる最低必要な時間以上長く試験
片を試料に接触させても、試験片内の微小空間（繊維質
構造体内部の繊維間隙及び支持板と繊維質構造体間に形
成される隙間）は試料液で満たされているため毛細管現
象による試料の吸い込みはそれ以上は実質上生じない。
このことは試験片内に吸い込まれる試料量が形成される
微小空間体積により規制されることを示しており、試験
片が試料を自動的に定量していることを示す。このため
本試験片を用いる測定に於いては、試料を一定量得るた
めのピペット操作等は不要になる。Therefore, the minimum time required for bringing the test piece into contact with the liquid sample depends on the sample used and various conditions at the time of preparing the test piece, but may be within 5 seconds, and the viscosity of the ink or the like is not so high. When using as a sample, about 1 second is enough. In addition, even if the test piece is contacted with the sample for a longer time than the minimum necessary time for contacting with the sample, a minute space in the test piece (fiber gap inside the fibrous structure and between the support plate and the fibrous structure is formed. Since the (gap) is filled with the sample liquid, the suction of the sample due to the capillary phenomenon does not substantially occur.
This indicates that the amount of the sample sucked into the test piece is regulated by the minute space volume formed, which indicates that the test piece automatically quantifies the sample. Therefore, in the measurement using this test piece, pipetting or the like for obtaining a fixed amount of sample is not necessary.

【００３４】また、従来のような試料点着タイプの試験
片を用いた場合には、大量の試料の点着により試験片の
裏側まで試料が漏れ、反射率読み取り装置を汚すと言っ
たトラブルを生じることがあったが、本発明のように吸
い込みタイプの試験片を用いると、このようなトラブル
は生じない。上記試料吸い上げ後、反射率読み取り装置
等に試験片をセットし反射率読み取りを行い、前述のク
ベルカ−ムンク式（１）に基づいてＫ／Ｓ値を求め、Ｋ
／Ｓ値と被分析物濃度の関係から試料中の被分析物濃度
を求める。Further, when a conventional sample spotting type test piece is used, there is a problem that the sample leaks to the back side of the test piece due to a large amount of sample spotting and the reflectance reading device is soiled. However, such trouble does not occur when a suction type test piece is used as in the present invention. After sucking up the sample, the test piece is set in a reflectance reading device and the reflectance is read, and the K / S value is calculated based on the Kubelka-Munk equation (1) described above.
The concentration of the analyte in the sample is determined from the relationship between the / S value and the concentration of the analyte.

【００３５】本発明に於いて作製した試験片を用い試料
中の被分析物濃度測定を行う際の反射率読み取り装置
は、例えば、図４に示されるように、光源６（試料中の
被分析物の吸収極大波長に対応する吸収極大を有する発
光ダイオード）、反射光検出器７、増幅器８、Ａ／Ｄ変
換器９、マイクロプロセッサ１０及び表示装置１１によ
り構成されたものを用いる。試験片セット時、装置上の
試験片をセットする面と、試験片の支持板の一面とはし
っかり接触し光源と光反射面の角度、距離等が変動しな
いように構成されている。本装置は反射光検出器上に試
験片がセットされると、直接光を照射し、反射光量を読
み取り、Ｋ／Ｓ値を算出し表示する。As shown in FIG. 4, a reflectance reading device for measuring the concentration of an analyte in a sample using the test piece produced in the present invention is, for example, as shown in FIG. A light emitting diode having an absorption maximum corresponding to the absorption maximum wavelength of an object, a reflected light detector 7, an amplifier 8, an A / D converter 9, a microprocessor 10, and a display device 11 is used. When the test piece is set, the surface of the device on which the test piece is set is firmly in contact with one surface of the support plate of the test piece so that the angle and distance between the light source and the light reflecting surface do not change. When the test piece is set on the reflected light detector, this device directly irradiates light, reads the amount of reflected light, calculates and displays the K / S value.

【００３６】このように、本発明の分析用試験片を用い
ることにより、液体試料の吸い込み、拡散が速やかであ
り、測光部位まで試料が容易に到達し、簡便、迅速な測
定が可能となる。また、試料吸い込み量が常に一定であ
るので、ピペット等を用いる定量計量操作は必要ない。As described above, by using the analytical test piece of the present invention, the liquid sample can be sucked and diffused quickly, and the sample can easily reach the photometric site, which enables simple and rapid measurement. Further, since the amount of sample sucked in is always constant, there is no need for quantitative measurement operation using a pipette or the like.

【００３７】[0037]

【発明の効果】以上のように、本発明の分析用試験片
は、水不浸透性支持板間に形成される繊維質構造体を含
む微小空間内に於いて、毛細管現象が生じやすいため、
液体試料の吸い込み、拡散が速やかであり、測定部位ま
で試料が容易に到達し、簡便、迅速かつ正確な、被分析
物の広い濃度範囲にわたる測定が可能である。また、本
試験片を用いる測定に於いては毛細管現象により吸い込
まれる試料量は試験片の構成により規制されているの
で、試料吸い込み量を常に一定にすることができ、ピペ
ット等を用いる定量計量操作は不要である。As described above, the analytical test piece of the present invention is apt to cause a capillary phenomenon in the minute space containing the fibrous structure formed between the water-impermeable support plates.
The liquid sample can be sucked and diffused quickly, and the sample can easily reach the measurement site, enabling simple, rapid, and accurate measurement over a wide concentration range of the analyte. Also, in the measurement using this test piece, the amount of sample sucked in due to the capillary phenomenon is regulated by the structure of the test piece, so the amount of sample sucked in can always be made constant, and quantitative measurement operation using a pipette, etc. Is unnecessary.

【００３８】本試験片は、染色業に於けるインクの配合
調整時のインク濃度測定、果汁飲料等の製造・品質管
理、血液中の有色成分濃度測定等に利用することが可能
である。また、本試験片を用いる測定に於いては、水平
に置かれた測定器上の試験片に液体試料を点着する必要
がないので、上腕、大腿、腹壁等身体の各部位から得ら
れるような微量の血液試料から、大きな発酵タンク内の
果汁等の試料まで測定することができる。This test piece can be used for measuring the ink concentration in the dyeing industry when adjusting the ink composition, for the production and quality control of fruit juice beverages, and for measuring the concentration of colored components in blood. In addition, in the measurement using this test piece, it is not necessary to spot the liquid sample on the test piece placed on the horizontally placed measuring instrument, so it is possible to obtain it from each part of the body such as the upper arm, thigh and abdominal wall. It is possible to measure from a very small amount of blood sample to a sample such as fruit juice in a large fermentation tank.

【００３９】以下、実施例により本発明を具体的に説明
する。 実施例１ 表１に記載した１０種類の素材を繊維質構造体として用
い、以下のようにして分析用試験片を作製し、水溶液中
のインク濃度測定を行った。The present invention will be specifically described below with reference to examples. Example 1 Ten kinds of materials shown in Table 1 were used as a fibrous structure to prepare an analytical test piece as described below, and the ink concentration in the aqueous solution was measured.

【００４０】分析用試験片の製造方法 表１に記載の繊維質構造体は、それぞれトライトンＸ１
００に浸した後乾燥し、５×１５ｍｍに裁断したものを
用いた。水不浸透性の支持体としては、厚さ１００μｍ
のポリエステルシートを用いた。該シートに両面粘着テ
ープを貼り、図５、に示す２種の形状に裁断した。
この２種の支持板を繊維質構造体を挟持するように貼り
合わせ分析用試験片とした（図５）。 Manufacturing Method of Analytical Test Pieces The fibrous structures shown in Table 1 are Triton X1.
After immersing in 00, it was dried and cut into 5 × 15 mm pieces. As a water-impermeable support, thickness 100 μm
The polyester sheet of was used. A double-sided adhesive tape was attached to the sheet and cut into two shapes shown in FIG.
The two types of support plates were laminated so as to sandwich the fibrous structure, and used as a test piece for analysis (FIG. 5).

【００４１】被測定用インク溶液の調製 蒸留水に既知量の青色インク（パイロット社製）を添加
し、各種濃度のインク溶液を得た。試験片の反射率読み取り装置 試料中の分析物濃度測定を行う際の分析装置は、前述し
た図４に示される装置を使用した。 Preparation of Ink Solution for Measurement A known amount of blue ink (manufactured by Pilot) was added to distilled water to obtain ink solutions of various concentrations. Reflectance Reading Device for Test Piece The analyzer shown in FIG. 4 was used as an analyzer for measuring the concentration of the analyte in the sample.

【００４２】上記で作製した試験片を用いて、各種イン
ク液の濃度測定及び測定精度評価を行った。すなわち、
試験片の試料吸い込み口を１〜２秒インク試料溶液に接
触した後、速やかに反射率読み取り装置にセットし、試
料吸い込み開始後１分経過後のＫ／Ｓ値を測定結果とし
て得た。尚、反射率１００％は標準白色板を用いて反射
率測定の度毎に設定した。The test pieces prepared above were used to measure the concentration of various ink liquids and evaluate the measurement accuracy. That is,
After the sample suction port of the test piece was brought into contact with the ink sample solution for 1 to 2 seconds, it was immediately set in the reflectance reading device, and the K / S value 1 minute after the start of sample suction was obtained as the measurement result. The reflectance of 100% was set for each reflectance measurement using a standard white plate.

【００４３】各試験片を用いてインク濃度を変化した試
料の測定を行った際のＫ／Ｓ値、及び３０倍希釈のイン
ク濃度の試料についてのそれぞれ試験片１０枚を用いて
の測定精度評価について、その結果を表２に示す。いず
れの試験片に於いてもインク濃度が高くなるにつれてＫ
／Ｓ値の増加が認められ、かつ実用上充分な精度が認め
られ、本発明の試験片がインク濃度測定に有効であるこ
とが示された。Evaluation of K / S value when measuring a sample in which the ink concentration was changed using each test piece, and measurement accuracy using 10 test pieces for each sample having an ink concentration of 30-fold dilution The results are shown in Table 2. In each test piece, K increased as the ink density increased.
It was confirmed that the / S value increased and that the accuracy was practically sufficient, and it was shown that the test piece of the present invention is effective for the ink density measurement.

【００４４】[0044]

【表１】 [Table 1]

【００４５】[0045]

【表２】 [Table 2]

【００４６】実施例２ 表３に示す単糸繊度１デニール以下の細繊度合成繊維よ
り成る繊維質構造体を用いる以外は、実施例１と同様に
して分析用試験片を作製し、更に、実施例１と同様な方
法により青色インク水溶液の測定を行い、Ｋ／Ｓ値及び
測定精度を求めた。結果を表４に示す。青色インク濃度
上昇に伴い、Ｋ／Ｓ値も上昇し良好な定量性が認めら
れ、本発明の試験片が高精度、かつ広範囲のインク濃度
測定に有効であることが示された。Example 2 An analytical test piece was prepared in the same manner as in Example 1 except that a fibrous structure composed of fine-fiber synthetic fibers having a single-fiber fineness of 1 denier or less as shown in Table 3 was used, and further carried out. The blue ink aqueous solution was measured by the same method as in Example 1 to determine the K / S value and the measurement accuracy. The results are shown in Table 4. As the blue ink density increased, the K / S value also increased, and good quantification was confirmed, demonstrating that the test piece of the present invention is highly accurate and effective for measuring ink density over a wide range.

【００４７】[0047]

【表３】 [Table 3]

【００４８】[0048]

【表４】 [Table 4]

【００４９】実施例３ 実施例２に於ける、単糸繊度０．０８〜０．５デニー
ル、ナイロン／ポリエステルの複合繊維（鐘紡社製：ザ
ヴィーナＭＸ）よりなる繊維質構造体を用いた試験片を
使用し、実施例１と同様な方法により黒色インク，ぶど
うジュースの濃度測定を行いＫ／Ｓ値を求めた。試料溶液の調製 黒色インク（国際チャート社製）、ブドウジュース（雪
印乳業社製）のそれぞれを蒸留水で段階的に希釈して各
種濃度の試料溶液を得た。結果を表５に示す。各試料溶
液の被分析物濃度上昇に伴い、Ｋ／Ｓ値も上昇し良好な
定量性が認められ、本発明の試験片が黒インク，ブドウ
ジュース濃度測定にも有効であることが示された。Example 3 A test piece using a fibrous structure of Example 2 consisting of a single fiber fineness of 0.08 to 0.5 denier and a nylon / polyester composite fiber (Kanabo Co., Ltd .: Zavina MX). Was used to measure the concentration of black ink and grape juice in the same manner as in Example 1 to determine the K / S value. Preparation of Sample Solution Each of black ink (manufactured by International Chart Co., Ltd.) and grape juice (manufactured by Snow Brand Milk Products Co., Ltd.) was diluted stepwise with distilled water to obtain sample solutions of various concentrations. The results are shown in Table 5. As the analyte concentration of each sample solution increased, the K / S value also increased and good quantification was observed, indicating that the test piece of the present invention is also effective for measuring the concentration of black ink and grape juice. .

【００５０】[0050]

【表５】 [Table 5]

【００５１】実施例４ 図２（ａ）に横断面を示す放射型の形状を示す５個のセ
グメントとしてポリアミド、該放射部を補完するくさび
型の形状を示す８個のセグメントとしてポリエステルを
用いた複合繊維４０ｄ／２５ｆを用いて４０Ｇ×３３”
の編み機でインターロック組成を編成した。この編物
を、濃度２０％ベンジルアルコールに浸漬してフィブリ
ル化処理し細繊度フィラメントを得た後、室温下オープ
ンソーパにて湯洗し、１８５℃でヒートセットし、表６
に示される製品密度及び厚さを有する編物を得た。次い
で、この編物を裁断し、実施例１と同様にして分析用試
験片を作製した。Example 4 Polyamide was used as five segments showing a radial shape whose cross section is shown in FIG. 2 (a), and polyester was used as eight segments showing a wedge shape which complements the radiation part. 40G x 33 "using composite fiber 40d / 25f
The interlock composition was knitted with the knitting machine. The knitted fabric was dipped in benzyl alcohol having a concentration of 20% to be fibrillated to obtain fine filaments, washed with hot water with an open soap at room temperature, and heat set at 185 ° C.
A knitted fabric having a product density and a thickness shown in Table 1 was obtained. Next, this knitted fabric was cut into a test piece for analysis in the same manner as in Example 1.

【００５２】上記分析用試験片を使用し、実施例１で使
用した青色インク溶液に、適当な粘度となるよう、ポリ
ピロリドンＫ９０を系中３．０％になるように添加した
試料を用い、試験片内に該インク溶液が吸い込まれる速
度を観察すると共に、測定の精度を評価した。なお、吸
い込み速度評価は肉眼で観察して行った。測定精度評価
は同一の試験片を１０枚ずつ作製し、インク原液を３０
倍希釈して上記により調製した試料を同様にして１０回
測定を行った結果よりＫ／Ｓの平均値、標準偏差を求め
て行った。Using the above-mentioned test piece for analysis, a sample was prepared by adding polypyrrolidone K90 to the blue ink solution used in Example 1 so as to have an appropriate viscosity so as to be 3.0% in the system. The speed at which the ink solution was sucked into the test piece was observed, and the accuracy of the measurement was evaluated. The suction speed was evaluated by visual observation. The measurement accuracy was evaluated by making 10 identical test pieces and
The average value and standard deviation of K / S were calculated from the results of 10 times measurement of the sample prepared by double-diluting and prepared in the same manner as above.

【００５３】結果を表６に示す。試料として適当な粘度
を有する溶液を用いた場合には、編み密度が６０００を
越えない方が、試料の吸い込みがスムーズに起こり好ま
しいことが示された。また、コースとウエルはほぼ揃
え、両者の積が３０００〜６０００としたとき、測定精
度が良好であることが示された。The results are shown in Table 6. It was shown that when a solution having an appropriate viscosity was used as a sample, it is preferable that the knitting density not exceed 6000 because the sample is smoothly sucked in. Further, it was shown that the measurement accuracy was good when the courses and wells were almost the same and the product of both was 3000 to 6000.

【００５４】[0054]

【表６】 [Table 6]

【００５５】実施例５ 支持板上の開口部の端の位置を表７に示した部位に設置
した以外は、実施例３と全く同様にして分析用試験片を
作製し、更に、実施例３で用いた３０倍希釈したブドウ
ジュースを試料として用い、試料の濃度測定を行い測定
の操作性及び測定精度を評価した。Example 5 An analytical test piece was prepared in the same manner as in Example 3 except that the positions of the ends of the openings on the support plate were set at the positions shown in Table 7, and Example 3 was further prepared. Using the 30-fold diluted grape juice used in 1. as a sample, the concentration of the sample was measured and the operability and measurement accuracy of the measurement were evaluated.

【００５６】結果を表７に示す。吸い込み口、開口部間
距離が０．５ｍｍの場合は測定精度は良好であるもの
の、開口部に直接試料が付着しないよう充分気を使わね
ばならなかった。試料量が限られており、適当な粘度を
有する試料を測定する場合に於いては吸い込み口、開口
部間距離が０．５〜１０ｍｍが適当であることが示され
た。The results are shown in Table 7. Although the measurement accuracy was good when the distance between the suction port and the opening was 0.5 mm, sufficient care had to be taken to prevent the sample from directly adhering to the opening. It has been shown that the amount of the sample is limited, and that the distance between the suction port and the opening is 0.5 to 10 mm when measuring a sample having an appropriate viscosity.

【００５７】[0057]

【表７】 [Table 7]

【００５８】実施例６ 実施例３と全く同様にして作製した分析用用試験片（Ｎ
ｏ．１）と、繊維質構造体表面には両面粘着テープを貼
り付けず繊維質構造体が支持板間に単に挟み込まれて保
持されるような形状に作製した試験片（Ｎｏ．２）に於
いて、試料溶液を吸い上げる時間を変化した際の測定に
於けるＫ／Ｓ値を評価した。尚、青色インク溶液は実施
例１と同様にして原液を３０倍希釈して用いた。Example 6 Analytical test piece (N
o. 1) and a test piece (No. 2) prepared in a shape such that the double-sided adhesive tape was not attached to the surface of the fibrous structure and the fibrous structure was simply sandwiched between the supporting plates and held. The K / S value in the measurement when the time for sucking up the sample solution was changed was evaluated. The blue ink solution was prepared by diluting the stock solution 30 times in the same manner as in Example 1.

【００５９】両試験片とも試料吸い込みに最低必要な接
触時間は２秒以内であり、表８に示されるごとく両試験
片とも試料接触時間によりＫ／Ｓ値が影響される程度は
小さく精度良く測定することが可能であり、試料接触時
間の変動は実用上問題にならないことが分かった。ま
た、Ｎｏ．１の試験片を用いた場合の方が試料接触時間
の影響はより小さく、高精度測定が要求される場合に於
いてはこの構成が好ましいことが示された。The minimum contact time required for sucking the sample in each of the test pieces was within 2 seconds, and as shown in Table 8, the K / S value was not affected by the sample contact time in both test pieces and was measured with high accuracy. It has been found that the fluctuation of the sample contact time does not pose a practical problem. In addition, No. It was shown that the influence of the sample contact time was smaller when the test piece No. 1 was used, and that this configuration is preferable when high precision measurement is required.

【００６０】[0060]

【表８】 [Table 8]

【００６１】実施例７ 実施例３と同様にして作製した分析用試験片（Ｎｏ．
１）（吸い込み口形状は図３）と、試料吸い込み口周
辺を図３のように支持板と繊維質構造体を密着させず
に作製した試験片（Ｎｏ．２）を用いて、血液を試料と
した際の試料吸い込み状況を評価した。結果を表９に示
す。両試験片に於いて試料の吸い込みは行われ、両者と
も実用上問題なかったが、試験片Ｎｏ．２の方が試料の
吸い込み開始が速やかに生じ、吸い込み操作の信頼性が
高いことが示された。Example 7 An analytical test piece (No.
1) (The shape of the suction port is shown in FIG. 3) and a test piece (No. 2) produced around the sample suction port without adhering the support plate and the fibrous structure as shown in FIG. The situation of sucking the sample was evaluated. The results are shown in Table 9. The sample was sucked into both the test pieces, and both had no problem in practical use. Sample No. 2 started to suck the sample more quickly, indicating that the sucking operation was more reliable.

【００６２】[0062]

【表９】 [Table 9]

【００６３】実施例８ 実施例３と同様にして分析用試験片を作製し、ヘマトク
リットの異なる血液を試料として用いた場合のＫ／Ｓ値
とヘマトクリットの関係を調べた。ヘマトクリットの異なる試料の調製 成人より、ヘパリン採血した静脈血を遠心分離で血球と
血漿に分離した。この血球，血漿を適宜混合しヘマトク
リットの異なる試料とした。Example 8 Analytical test pieces were prepared in the same manner as in Example 3, and the relationship between the K / S value and hematocrit when bloods having different hematocrit were used as samples was examined. Preparation of Samples with Different Hematocrit From adults, heparin-collected venous blood was separated into blood cells and plasma by centrifugation. The blood cells and plasma were appropriately mixed to prepare samples with different hematocrit.

【００６４】表１０より明らかなように、血球中のヘマ
トクリット増加に対応し、Ｋ／Ｓ値の変化が認められ、
本試験片が血液中ヘマトクリット測定に有効に用い得る
ことが示された。As is clear from Table 10, a change in K / S value was observed in response to an increase in hematocrit in blood cells.
It was shown that this test piece can be effectively used for hematocrit measurement in blood.

【００６５】[0065]

【表１０】 [Table 10]

【図面の簡単な説明】[Brief description of drawings]

【図１】本発明の分析用試験片の一実施態様を示す説明
図。FIG. 1 is an explanatory view showing an embodiment of an analytical test piece of the present invention.

【図２】本発明で使用する繊維質構造体を構成するフィ
ブリル化複合繊維の横断面を示す説明図。FIG. 2 is an explanatory view showing a cross section of a fibrillated composite fiber which constitutes a fibrous structure used in the present invention.

【図３】本発明の分析用試験片の試料吸い込み口周辺部
を示す説明図。FIG. 3 is an explanatory diagram showing a portion around a sample suction port of the analytical test piece of the present invention.

【図４】本発明の分析用試験片評価に使用した反射率読
み取り装置の概略説明図。FIG. 4 is a schematic explanatory view of a reflectance reading device used for evaluation of an analytical test piece of the present invention.

【図５】本発明の分析用試験片の構成を示す説明図。FIG. 5 is an explanatory view showing the constitution of the analytical test piece of the present invention.

【符号の説明】[Explanation of symbols]

１ 繊維質構造体 ２ 水不浸透性支持板 ３ 試料吸い込み口 ４ 測定用開口部 1 Fibrous structure 2 Water impermeable support plate 3 Sample suction port 4 Measurement opening

───────────────────────────────────────────────────── フロントページの続き (72)発明者 高瀬 清 兵庫県西宮市上田東町４番38−404号 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kiyoshi Takase No. 38-404 Uedahigashi-cho, Nishinomiya-shi, Hyogo Prefecture

Claims (2)

【特許請求の範囲】[Claims] 【請求項１】 液体試料中の成分を分析するための試験
片であって、薄片状の繊維質構造体を、その一端が液体
試料吸い込み口となるよう、２枚の水不浸透性支持板間
に三者端部が略揃うようにして挾持し、更に、該水不浸
透性支持板の一方に、繊維質構造体の着色程度を計測す
るための測定用開口部を設けてなることを特徴とする分
析用試験片。1. A test piece for analyzing components in a liquid sample, comprising two thin water-impervious support plates having a thin fibrous structure so that one end thereof serves as a liquid sample suction port. The three end portions are sandwiched so that they are substantially aligned with each other, and further, one of the water-impermeable support plates is provided with a measurement opening for measuring the degree of coloring of the fibrous structure. Characteristic test piece for analysis. 【請求項２】 繊維質構造体を構成する繊維が、１デニ
ール以下の合成繊維フィラメントであることを特徴とす
る請求項１記載の分析用試験片。2. The analytical test piece according to claim 1, wherein the fibers constituting the fibrous structure are synthetic fiber filaments having a denier of 1 or less.