JPH0727760A - Blood-separating agent and blood-separating tube - Google Patents
Blood-separating agent and blood-separating tubeInfo
- Publication number
- JPH0727760A JPH0727760A JP17030893A JP17030893A JPH0727760A JP H0727760 A JPH0727760 A JP H0727760A JP 17030893 A JP17030893 A JP 17030893A JP 17030893 A JP17030893 A JP 17030893A JP H0727760 A JPH0727760 A JP H0727760A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- separating agent
- separation
- separating
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は，血液分離剤に関する。
血液分離剤は，血液検査に用いる真空採血管の管底に収
容して使用される。血液が注入された採血管は，遠心分
離により血清部分と血餅部分あるいは血漿部分と血球部
分とにその比重差により分離される。その際，両成分の
中間的な比重を付与する事により両成分の間に隔壁を形
成せしめ，これら両成分の分離操作を容易にする目的に
使用し得る血液分離剤及びこれを有底管内に収容してな
る血液分離管に関する。FIELD OF THE INVENTION The present invention relates to a blood separating agent.
The blood separating agent is used by being housed in the bottom of a vacuum blood collection tube used for blood tests. A blood collection tube into which blood is injected is separated into a serum portion and a blood clot portion or a plasma portion and a blood cell portion by centrifugal separation due to the difference in specific gravity. At that time, a blood separating agent that can be used for the purpose of facilitating the separation operation of these two components by forming a partition wall between them by giving an intermediate specific gravity of both components The present invention relates to a blood separation tube housed therein.
【０００２】[0002]
【従来の技術】従来，血液の分離操作に用いられる分離
剤としては，シリコ−ンオイル，塩素化ポリブテン，ポ
リイソブテン，アクリル重合体，α−オレフィン／マレ
イン酸ジエステル重合体などの樹脂を主成分として，こ
れらに比重，粘度調整用としてシリカ，粘土等の無機微
粒子や有機ゲル化剤を添加したものがあった。これら，
主成分となる樹脂は基本的に疎水性を有するものであ
り，また，無機微粒子は増粘効果，チキソトロピ−性付
与を与え，有機ゲル化剤はチキソトロピ−性を付与する
ことなく，増粘効果を与えるものである。2. Description of the Related Art Conventionally, as a separating agent used for separating blood, a resin such as silicone oil, chlorinated polybutene, polyisobutene, acrylic polymer, α-olefin / maleic acid diester polymer is used as a main component. In some of these, inorganic fine particles such as silica and clay or an organic gelling agent were added for adjusting specific gravity and viscosity. these,
The resin as the main component is basically hydrophobic, and the inorganic fine particles give a thickening effect and a thixotropic property, and the organic gelling agent gives a thixotropic property without giving a thixotropic property. Is to give.
【０００３】[0003]
【発明が解決しようとする課題】しかし，無機微粒子は
その比重値が一般的に高く，主成分となる樹脂との比重
差が大きく，採血後の遠心分離操作時に遠心力により無
機物と樹脂が分離し，分離剤としての比重が変化し，下
層にある血餅部分と上層にある血清部分あるいは血球部
分と血漿部分との中間層にバリヤ−形成をしなくなる問
題が生じることがあった。また，無機物は表面活性が高
く，血液中の成分やいくつかの薬剤を吸着させ検査結果
に異常を生じさせる場合が有り，使用可能な無機物には
限りがあった。However, the specific gravity of inorganic fine particles is generally high, and the difference in specific gravity from the resin as the main component is large, and the inorganic matter and the resin are separated by centrifugal force during centrifugation operation after blood collection. However, the specific gravity of the separating agent may change, and there may be a problem that barrier formation is not formed in the intermediate layer between the blood clot portion in the lower layer and the serum portion in the upper layer or the blood cell portion and the plasma portion. In addition, inorganic substances have high surface activity, and sometimes components in blood or some drugs may be adsorbed to cause abnormal test results, and usable inorganic substances are limited.
【０００４】また，有機ゲル化剤を用いると，ゲルの粘
度が経時で変化するため，保存安定性に劣ると言う問題
点があった。Further, when an organic gelling agent is used, the viscosity of the gel changes with time, so that there is a problem that the storage stability is poor.
【０００５】[0005]
【課題を解決するための手段】本発明の目的は，これら
欠点のない，実用性に優れた血液分離剤及びそれを有底
管内に収容して得られる血液分離管を提供することにあ
る。本発明者らは，かかる目的を達成する為に鋭意検討
を進めた結果，本発明に至った。即ち，本発明は，分離
層形成材料及び数平均分子量１０００〜１００００であ
る変成ポリエチレンまたは／および変成ポリプロピレン
を含むことを特徴とする血液分離剤である。SUMMARY OF THE INVENTION An object of the present invention is to provide a blood separating agent which does not have these drawbacks and is excellent in practical use, and a blood separating tube obtained by accommodating the blood separating agent in a bottomed tube. The present inventors arrived at the present invention as a result of intensive studies to achieve the above object. That is, the present invention is a blood separating agent comprising a separation layer forming material and modified polyethylene or / and modified polypropylene having a number average molecular weight of 1,000 to 10,000.
【０００６】以下，本発明について詳細に説明する。本
発明の血液分離剤に用いる分離層形成材料としては，そ
の比重が血清部分と血餅部分、あるいは血漿部分と血球
部分との中間領域にあるものであれば，低粘度の各種有
機溶剤や可塑剤から高粘度の高分子油状物まで使用する
ことが出来る。実用上，安定で適度な流動性及びゲル化
性を与える点で、好ましくは温度２５℃での粘度が２０
０〜６０万ｃｐｓの範囲の高分子油状物が適している。
かかる高分子油状物としては，例えば，シリコ−ン，塩
素化ポリブテン，塩素化ポリブタジエン，ポリ（メタ）
アクリル酸エステル，ポリイソブテン，α−オレフィン
の重合体，ポリスチレン，α−オレフィンまたはスチレ
ンとマレイン酸ジエステルとの共重合体などが挙げられ
る。The present invention will be described in detail below. As the separation layer forming material used in the blood separating agent of the present invention, as long as its specific gravity is in the intermediate region between the serum portion and blood clot portion, or the plasma portion and blood cell portion, various low-viscosity organic solvents and plastic From agents to high-viscosity polymer oils can be used. In terms of practical use, the viscosity at a temperature of 25 ° C. is preferably 20 from the viewpoint of giving stable and appropriate fluidity and gelling property.
Polymeric oils in the range 0 to 600,000 cps are suitable.
Examples of such polymer oils include silicone, chlorinated polybutene, chlorinated polybutadiene, poly (meth)
Examples thereof include acrylic acid ester, polyisobutene, a polymer of α-olefin, polystyrene, a copolymer of α-olefin or styrene and maleic acid diester.
【０００７】本発明に用いられる変成ポリエチレン及び
変成ポリプロピレンは，血液分離剤として用いられる前
述の分離層形成材料に混合分散することにより，安定な
コロイド構造を形成するか，または顔料や充填剤に吸着
してフロキュレ−ション構造を作り，分離層形成材料に
粘性付与及びチキソトロピックな性質を与える。上記変
成ポリエチレン及び変成ポリプロピレンとしては、その
分子構造中にリン酸基、スルホン基、スルホベタイン、
カルボキシル基、カルボニル基、水酸基、ニトロ基、フ
ェニル基、アミノ基等の官能基あるいはハロゲン原子、
マレイン酸、無水マレイン酸、マレイン酸ジエステル化
物、ジカルボン酸、長鎖脂肪酸等を含有し、かつ比重は
０．８〜１．０、数平均分子量は１０００〜１００００
を有するものが挙げられる。更に、好ましくは比重は
０．８５〜０．９５、数平均分子量は１０００〜４００
０であることが望ましい。The modified polyethylene and modified polypropylene used in the present invention form a stable colloidal structure or are adsorbed on a pigment or a filler by mixing and dispersing with the above-mentioned separation layer forming material used as a blood separating agent. Then, a flocculation structure is formed to give viscosity and thixotropic properties to the material for forming the separation layer. As the modified polyethylene and modified polypropylene, a phosphate group, a sulfone group, a sulfobetaine in the molecular structure,
Functional groups such as carboxyl group, carbonyl group, hydroxyl group, nitro group, phenyl group, amino group or halogen atom,
It contains maleic acid, maleic anhydride, maleic acid diester, dicarboxylic acid, long chain fatty acid, etc., and has a specific gravity of 0.8 to 1.0 and a number average molecular weight of 1,000 to 10,000.
And the like. Furthermore, the specific gravity is preferably 0.85 to 0.95, and the number average molecular weight is 1000 to 400.
It is preferably 0.
【０００８】これら変成ポリエチレン及び変成ポリプロ
ピレンは、分離層形成材料との相溶性、粘度，比重等を
考慮して配合しなければならないが、通常は分離層形成
材料１００重量部に対して０．１〜５０重量部，好まし
くは１〜１０重量部添加することが望ましい。本発明の
血液分離剤を構成する組成物の好適な物性は，温度２５
℃における比重が血清と血餅あるいは血漿と血球の中
間、即ち１．０３０〜１．０６０であり、粘度は２０万
〜２００万ｃｐｓ，降伏値１００〜２０００ｄｙｎｅ／
ｃｍ2 の範囲が適当である。These modified polyethylene and modified polypropylene must be blended in consideration of compatibility with the material for forming the separation layer, viscosity, specific gravity, etc., but usually 0.1 to 100 parts by weight of the material for forming the separation layer. It is desirable to add -50 parts by weight, preferably 1-10 parts by weight. The preferred physical properties of the composition constituting the blood separating agent of the present invention are as follows:
The specific gravity at ℃ is between blood serum and blood clot or blood plasma and blood cells, ie, 1.030 to 1.060, viscosity is 200,000 to 2,000,000 cps, and yield value is 100 to 2000 dyne /
A range of cm 2 is suitable.
【０００９】未変成のポリエチレン及びポリプロピレン
を使用すると、分離層形成材料との親和性が乏しく、遠
心分離時に低比重のポリエチレン及びポリプロピレンの
みが分離し血液層上部に浮上し分離剤としての性能を発
揮しない。本発明による血液分離剤は場合により、粘
土、シリカ、マイカ、タルク、クレ−、珪藻土、ベント
ナイト、酸化チタン、炭酸カルシウム等の無機粉末を適
宜添加してもよい。When unmodified polyethylene and polypropylene are used, the affinity for the material for forming the separation layer is poor, and only polyethylene and polypropylene having a low specific gravity separates during centrifugation and floats above the blood layer to exhibit the performance as a separating agent. do not do. Inorganic powders such as clay, silica, mica, talc, clay, diatomaceous earth, bentonite, titanium oxide and calcium carbonate may be appropriately added to the blood separating agent according to the present invention.
【００１０】[0010]
【実施例】本発明を実施例に基づいて説明する。例中、
「部」「％」は、それぞれ「重量部」「重量％」であ
る。 実施例１ 炭素数１２及び１４の混合α−オレフィンとマレイン酸
ジエステル共重合物１００部、無水マレイン酸導入変成
ポリエチレン（数平均分子量２０００）６部、ベントナ
イト１部を３本ロ−ルにより混練りし、粘度３０万ｃｐ
ｓ、降伏値８００ｄｙｎｅ／ｃｍ2 、比重１．０３２の
血液分離剤を得た。EXAMPLES The present invention will be described based on examples. In the example,
"Parts" and "%" are "parts by weight" and "% by weight", respectively. Example 1 100 parts of a mixed α-olefin having 12 and 14 carbon atoms and a maleic acid diester copolymer, 6 parts of maleic anhydride-introduced modified polyethylene (number average molecular weight 2000), and 1 part of bentonite were kneaded with 3 rolls. And viscosity 300,000 cp
s, yield value 800 dyne / cm 2 , and specific gravity 1.032 were obtained.
【００１１】得られた分離剤は１５ｃｃ試験管に１ｇ入
れ、さらに全血試料１０ｃｃ入れ、１日放置後、１２０
０Ｇ１０分間遠心分離したところ、分離剤は血球部分と
血漿部分との中間層に位置し、分離剤からの変性ポリエ
チレンの分離は見られなかった。 実施例２ 炭素数１２及び１４の混合α−オレフィンとマレイン酸
ジエステル共重合物１００部、無水マレイン酸導入変性
ポリプロピレン（数平均分子量３５００）８部、シリカ
粉４部を３本ロ−ルにより混練りし、粘度４０万ｃｐ
ｓ、降伏値３５０ｄｙｎｅ／ｃｍ2 、比重１．０４５の
血液分離剤を得た。得られた分離剤は１５ｃｃ試験管に
１ｇ入れ、さらに全血試料１０ｃｃ入れ、１日放置後、
１２００Ｇ１０分間遠心分離したところ、分離剤は血餅
部分と血清部分との中間層に位置し、分離剤からの変性
ポリプロピレンの分離は見られなかった。1 g of the obtained separating agent was put in a 15 cc test tube, 10 cc of a whole blood sample was further put therein, and left for 1 day.
Upon centrifugation at 0G for 10 minutes, the separating agent was located in the intermediate layer between the blood cell portion and the plasma portion, and separation of the modified polyethylene from the separating agent was not observed. Example 2 100 parts of a mixed α-olefin having 12 and 14 carbon atoms and a maleic acid diester copolymer, 8 parts of maleic anhydride-introduced modified polypropylene (number average molecular weight 3500), and 4 parts of silica powder were mixed with 3 rolls. Knead, viscosity 400,000 cp
s, yield value 350 dyne / cm 2 , and specific gravity 1.045 were obtained. 1 g of the obtained separating agent was placed in a 15 cc test tube, 10 cc of a whole blood sample was further placed, and after leaving for 1 day,
Upon centrifugation at 1200 G for 10 minutes, the separating agent was located in the intermediate layer between the blood clot portion and the serum portion, and separation of the modified polypropylene from the separating agent was not observed.
【００１２】実施例３ スチレンオリゴマ−１００部、フェニル基導入変成ポリ
エチレン（数平均分子量８０００）５部、シリカ粉４部
を３本ロ−ルにより混練りし、粘度２８万ｃｐｓ、降伏
値３２０ｄｙｎｅ／ｃｍ2 、比重１．０４４の血液分離
剤を得た。得られた分離剤は１５ｃｃ試験管に１ｇ入
れ、さらに全血試料１０ｃｃ入れ、１日放置後、１２０
０Ｇ１０分間遠心分離したところ、分離剤は血餅部分と
血清部分との中間層に位置し、分離剤からの変成ポリエ
チレンの分離は見られなかった。 比較例１ 炭素数１２及び１４の混合α−オレフィンとマレイン酸
ジエステル共重合物１００部、未変性ポリエチレン（数
平均分子量２０００）３部を３本ロ−ルにより混練り
し、粘度２５万ｃｐｓ、降伏値５００ｄｙｎｅ／ｃ
ｍ2 、比重１．０３３の血液分離剤を得た。得られた分
離剤は１５ｃｃ試験管に１ｇ入れ、さらに全血試料１０
ｃｃ入れ、１日放置後、１２００Ｇ１０分間遠心分離し
たところ、未変成ポリエチレンのみが血漿部分に浮遊
し、分離層を形成しなかった。Example 3 100 parts of styrene oligomer, 5 parts of phenyl group-modified modified polyethylene (number average molecular weight of 8000) and 4 parts of silica powder were kneaded with 3 rolls to give a viscosity of 280,000 cps and a yield value of 320 dyne /. A blood separating agent having a cm 2 and a specific gravity of 1.044 was obtained. 1 g of the obtained separating agent was placed in a 15 cc test tube, and 10 cc of a whole blood sample was further placed.
Upon centrifugation at 0G for 10 minutes, the separating agent was located in the intermediate layer between the blood clot portion and the serum portion, and separation of the modified polyethylene from the separating agent was not observed. Comparative Example 1 100 parts of a mixed α-olefin having 12 and 14 carbon atoms, a maleic acid diester copolymer, and 3 parts of unmodified polyethylene (number average molecular weight of 2000) were kneaded with 3 rolls to give a viscosity of 250,000 cps. Yield value 500 dyne / c
A blood separating agent having m 2 and a specific gravity of 1.033 was obtained. 1 g of the obtained separating agent was placed in a 15 cc test tube, and the whole blood sample 10
After cc was put in and left for 1 day and then centrifuged at 1200 G for 10 minutes, only unmodified polyethylene floated in the plasma part and a separation layer was not formed.
【００１３】比較例２ ポリイソブテン１００部、シリカ粉１０部、沈降性硫酸
バリウム１５部を３本ロ−ルにより混練りし、粘度４０
万ｃｐｓ、降伏値４００ｄｙｎｅ／ｃｍ2 、比重１．０
４６の血液分離剤を得た。得られた分離剤は１５ｃｃ試
験管に１ｇ入れさらに全血試料１０ｃｃ入れ、１日放置
後、１２００Ｇ１０分間遠心分離したところ、分離剤中
から沈降性硫酸バリウムが脱離し、遠沈管の低部に沈降
して十分な分離層を形成しなかった。Comparative Example 2 100 parts of polyisobutene, 10 parts of silica powder, and 15 parts of precipitated barium sulfate were kneaded with 3 rolls to give a viscosity of 40.
10,000 cps, yield value 400 dyne / cm 2 , specific gravity 1.0
Forty-six blood separating agents were obtained. 1 g of the obtained separating agent was placed in a 15 cc test tube, 10 cc of whole blood sample was added, and the mixture was left for 1 day and then centrifuged at 1200 G for 10 minutes. Did not form a sufficient separation layer.
【００１４】[0014]
【発明の効果】本発明の血液分離剤を用いることにより
良好な隔壁を形成せしめ，血液の分離操作を容易にする
ことが可能となった。EFFECTS OF THE INVENTION By using the blood separating agent of the present invention, it becomes possible to form a good partition wall and facilitate the operation of separating blood.
Claims (3)
０〜１００００である変成ポリエチレンまたは／および
変成ポリプロピレンを含むことを特徴とする血液分離
剤。1. A material for forming a separation layer and a number average molecular weight of 100.
A blood separating agent comprising modified polyethylene or / and modified polypropylene of 0 to 10000.
おける比重が１．０３０〜１．０６０であることを特徴
とする血液分離剤。2. The blood separating agent according to claim 1, wherein the specific gravity at 25 ° C. is 1.030 to 1.060.
収容してなる血液分離管。3. A blood separation tube containing the blood separation agent according to claim 1 in a bottomed tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17030893A JPH0727760A (en) | 1993-07-09 | 1993-07-09 | Blood-separating agent and blood-separating tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17030893A JPH0727760A (en) | 1993-07-09 | 1993-07-09 | Blood-separating agent and blood-separating tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0727760A true JPH0727760A (en) | 1995-01-31 |
Family
ID=15902570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17030893A Pending JPH0727760A (en) | 1993-07-09 | 1993-07-09 | Blood-separating agent and blood-separating tube |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0727760A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007029525A1 (en) * | 2005-09-09 | 2007-03-15 | Toagosei Co., Ltd. | Polymer for blood separating media and blood separating medium compositions |
-
1993
- 1993-07-09 JP JP17030893A patent/JPH0727760A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007029525A1 (en) * | 2005-09-09 | 2007-03-15 | Toagosei Co., Ltd. | Polymer for blood separating media and blood separating medium compositions |
| JPWO2007029525A1 (en) * | 2005-09-09 | 2009-03-19 | 東亞合成株式会社 | Polymer for blood separating agent and blood separating agent composition |
| JP4510893B2 (en) * | 2005-09-09 | 2010-07-28 | 東亞合成株式会社 | Polymer for blood separating agent and blood separating agent composition |
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