JPH07260799A - Distribution device - Google Patents
Distribution deviceInfo
- Publication number
- JPH07260799A JPH07260799A JP6076699A JP7669994A JPH07260799A JP H07260799 A JPH07260799 A JP H07260799A JP 6076699 A JP6076699 A JP 6076699A JP 7669994 A JP7669994 A JP 7669994A JP H07260799 A JPH07260799 A JP H07260799A
- Authority
- JP
- Japan
- Prior art keywords
- tip
- liquid
- gas
- reaction container
- supplied
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1011—Control of the position or alignment of the transfer device
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明は、検査液等の液体が収
容された試料容器内からピペッターによって液体を採取
し、このように採取した液体をピペッター先端のチップ
から被検査試料等が収容された反応容器に分注させる分
注装置に係り、特に、液体をピペッター先端のチップか
ら反応容器に供給する際に、液体がピペッター先端のチ
ップに付着して反応容器に供給されずに残るということ
がなく、液体を正確に反応容器に分注させることのでき
る分注装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention collects a liquid with a pipetter from a sample container containing a liquid such as a test liquid, and stores the sampled liquid from the tip of the pipettor. Dispensing device for dispensing into a reaction container, especially when liquid is supplied from the tip of the pipettor tip to the reaction container, the liquid adheres to the tip of the pipetter tip and remains without being supplied to the reaction vessel. The present invention relates to a dispensing device capable of accurately dispensing a liquid into a reaction container.
【０００２】[0002]
【従来の技術】従来より、血液等の各種の被検査試料を
検査する場合、一般に、分注装置により試料容器内に収
容された検査液等の液体を採取し、このように採取した
液体を被検査試料が収容された分注用プレート等の反応
容器における収容部に分注させて検査するようにしてい
た。2. Description of the Related Art Conventionally, when inspecting various test samples such as blood, generally, a liquid such as a test liquid contained in a sample container is sampled by a dispensing device, and the sampled liquid is collected. The sample to be inspected is dispensed to the accommodation part of the reaction container such as a dispensing plate for inspection.
【０００３】そして、上記のように分注装置により、採
取した液体を被検査試料が収容された反応容器の収容部
に分注させるにあたっては、一般に、図１の（Ａ），
（Ｂ）に示すように、ピペッター２０先端のチップ２１
を試料容器１０に収容された検査液１１中に挿入させ
て、試料容器１０から所定量の検査液１１を採取した
後、このピペッター２０を分注用プレート等の反応容器
３０に導いて、このピペッター２０先端のチップ２１を
被検査液３１が収容された反応容器３０の収容部３２内
に挿入し、採取した検査液１１をピペッター２０のチッ
プ２１から反応容器３０の各収容部３２に一定量供給し
て分注させるようにしていた。In order to dispense the collected liquid into the accommodation portion of the reaction container in which the sample to be inspected is accommodated by the dispensing device as described above, generally, in FIG.
As shown in (B), the tip 21 of the tip of the pipettor 20.
Is inserted into the test liquid 11 contained in the sample container 10 to collect a predetermined amount of the test liquid 11 from the sample container 10, and then the pipettor 20 is introduced into a reaction container 30 such as a dispensing plate, The tip 21 at the tip of the pipettor 20 is inserted into the accommodating portion 32 of the reaction container 30 in which the liquid to be inspected 31 is accommodated, and the collected inspection liquid 11 is transferred from the tip 21 of the pipetter 20 to each accommodating portion 32 of the reaction container 30 in a fixed amount. It was supplied and dispensed.
【０００４】ここで、上記のように採取した検査液１１
をピペッター２０先端のチップ２１から反応容器３０の
収容部３２に供給するようにした場合、検査液１１がそ
の表面張力によって、図２に示すように球状の液滴にな
ってピペッター２０先端のチップ２１に付着して残り、
反応容器３０の収容部３２に供給されず、一定量の検査
液１１を各収容部３２に正確に供給して検査することが
できなくなるという問題があり、特に、１μｌ以下の微
量の検査液１１を反応容器３０の収容部３２に分注させ
て検査を行う場合において、上記のように検査液１１が
球状の液滴になってピペッター２０先端のチップ２１に
付着すると、検査液１１が反応容器３０の収容部３２に
供給されなくなって検査が行えなくなるという問題があ
った。Here, the test liquid 11 collected as described above
When the liquid is supplied from the tip 21 at the tip of the pipettor 20 to the accommodating portion 32 of the reaction container 30, the test liquid 11 becomes spherical droplets as shown in FIG. 21 sticks and remains,
There is a problem that a fixed amount of the test liquid 11 cannot be accurately supplied to each of the storage parts 32 for inspection without being supplied to the storage part 32 of the reaction container 30, and in particular, a very small amount of the test liquid 11 of 1 μl or less. When the test liquid 11 is dispensed to the accommodation portion 32 of the reaction container 30 and is inspected, and the test liquid 11 becomes spherical droplets and adheres to the tip 21 at the tip of the pipettor 20 as described above, the test liquid 11 becomes the reaction container. There is a problem that the inspection cannot be performed because the supply to the accommodating portion 32 of 30 is stopped.
【０００５】そこで、従来においては、上記のようにピ
ペッター２０のチップ２１に付着した検査液１１の液滴
を反応容器３０における収容部３２に供給するため、ピ
ペッター２０を移動させて、図３に示すように、チップ
２１に付着した検査液１１の液滴を反応容器３０の収容
部３２に収容された被検査液３１の液面に接触させて収
容部３２に供給するようにしたり、図４に示すように、
チップ２１に付着した検査液１１の液滴を反応容器３０
における収容部３２の内壁面３２ａに接触させて収容部
３２に供給するようにしていた。Therefore, in the prior art, in order to supply the liquid droplets of the test liquid 11 adhering to the chip 21 of the pipettor 20 to the accommodating portion 32 of the reaction container 30 as described above, the pipetter 20 is moved to the position shown in FIG. As shown, the droplets of the test liquid 11 adhering to the chip 21 may be brought into contact with the liquid surface of the test liquid 31 stored in the storage portion 32 of the reaction container 30 and supplied to the storage portion 32. As shown in
Droplets of the test liquid 11 adhering to the chip 21 are added to the reaction container 30.
It was made to contact the inner wall surface 32a of the accommodating part 32 in and to supply to the accommodating part 32.
【０００６】しかし、上記のようにピペッター２０を移
動させて、チップ２１に付着した検査液１１の液滴を反
応容器３０の収容部３２に収容された被検査液３１の液
面に接触させる操作は困難で、特に、自動的にこのよう
な操作を行うことは非常に困難であり、チップ２１に付
着した検査液１１の液滴が被検査液３１の液面に接触さ
れずに残ったり、またピペッター２０を下方に移動させ
過ぎたために、チップ２１が収容部３２に収容された被
検査液３１に接触して、チップ２１に被検査液３１が付
着してしまい、その後の検査に支障をきたす等の問題が
あり、またこのため、チップ２１に被検査液３１が付着
した場合には、チップ２１を取り換えなければならなく
なる等の問題も生じた。However, the operation of moving the pipetter 20 as described above to bring the droplets of the test liquid 11 adhering to the chip 21 into contact with the liquid surface of the test liquid 31 contained in the container 32 of the reaction container 30. In particular, it is very difficult to perform such an operation automatically, and the droplet of the test liquid 11 attached to the chip 21 remains without coming into contact with the liquid surface of the test liquid 31, Further, since the pipette 20 is moved too downward, the chip 21 comes into contact with the liquid 31 to be inspected stored in the accommodating portion 32, and the liquid 31 to be inspected adheres to the chip 21, which hinders the subsequent inspection. There is also a problem that the chip 21 needs to be replaced when the test liquid 31 adheres to the chip 21.
【０００７】また、チップ２１に付着した検査液１１の
液滴を収容部３２の内壁面３２ａに接触させて収容部３
２に供給させる場合、チップ２１を収容部３２の内壁面
３２ａに強く押し付けなければ、チップ２１に付着した
検査液１１の液滴がうまく収容部３２の内壁面３２ａに
接触されず、この液滴を安定して収容部３２に供給する
ことができない等の問題があった。Further, the liquid droplets of the test liquid 11 adhering to the chip 21 are brought into contact with the inner wall surface 32a of the accommodating portion 32 so that the accommodating portion 3 can be obtained.
2 is supplied to the inner wall surface 32a of the accommodating portion 32, the droplets of the test liquid 11 adhering to the chip 21 are not properly brought into contact with the inner wall surface 32a of the accommodating portion 32. However, there is a problem in that the oil cannot be stably supplied to the accommodating portion 32.
【０００８】[0008]
【発明が解決しようとする課題】この発明は、検査液等
の液体を試料容器からピペッターによって採取し、この
ように採取した液体をピペッター先端のチップから被検
査試料等が収容された反応容器に分注させる分注装置に
おける上記のような問題を解決することを課題とするも
のである。SUMMARY OF THE INVENTION The present invention collects a liquid such as a test liquid from a sample container by a pipettor, and collects the liquid thus collected from a tip of the pipettor into a reaction container containing a sample to be inspected. It is an object of the present invention to solve the above problems in a dispensing device for dispensing.
【０００９】すなわち、この発明は、上記のような分注
装置において、ピペッターによって採取した液体をピペ
ッター先端のチップから反応容器に一定量供給して、反
応容器に分注させるにあたり、ピペッターのチップに反
応容器に供給する液体の液滴が付着して反応容器に供給
されなくなるということがなく、正確な量の液体を反応
容器に簡単且つ確実に供給して正確な検査が行えるよう
になり、特に、１μｌ以下の微量な量の液体を反応容器
に分注させる場合にも、この液体を反応容器に確実に供
給して検査が行えるようになると共に、このような分注
操作を自動化できるようにすることを課題とするもので
ある。That is, according to the present invention, in the above dispenser, a predetermined amount of the liquid collected by the pipettor is supplied from the tip of the pipettor to the reaction container and dispensed into the reaction container. It is possible to supply a precise amount of liquid to the reaction container easily and reliably for accurate inspection without the liquid droplets to be supplied to the reaction container adhering to the reaction container and not being supplied to the reaction container. Even when a small amount of liquid of 1 μl or less is dispensed into the reaction container, the liquid can be reliably supplied to the reaction container for inspection, and such dispensing operation can be automated. The task is to do so.
【００１０】[0010]
【課題を解決するための手段】この発明においては、上
記のような課題を解決するため、ピペッターによって採
取した液体をこのピペッター先端のチップから反応容器
に供給して分注を行う分注装置において、採取した液体
をピペッター先端のチップから反応容器に供給する際
に、このチップの周囲に気体を吹き付ける気体噴出手段
を設けるようにしたのである。In order to solve the above-mentioned problems, the present invention provides a dispensing apparatus for supplying a liquid collected by a pipettor from a tip of the pipettor to a reaction container for dispensing. When the collected liquid is supplied from the tip of the pipettor to the reaction vessel, a gas jetting means for blowing a gas around the tip is provided.
【００１１】[0011]
【作用】この発明における分注装置においては、ピペッ
ターによって採取した液体をこのピペッター先端のチッ
プから反応容器に供給する際に、気体噴出手段からこの
ピペッター先端のチップの周囲に気体を吹き付けるよう
にする。In the dispensing apparatus according to the present invention, when the liquid collected by the pipettor is supplied from the tip of the pipettor to the reaction container, gas is blown from the gas jetting means to the periphery of the tip of the pipetter. .
【００１２】このようにすると、表面張力によってピペ
ッター先端のチップに球状になって残る液滴が、上記気
体の吹き付け力や気体の流れによる吸引力によってチッ
プの先端から離脱されて反応容器に供給されるようにな
り、従来のように、ピペッター先端のチップに液体の液
滴が残って反応容器に供給されなくなるということがな
く、一定量の液体が確実に反応容器に供給されるように
なる。In this way, the droplets, which are spherical and remain on the tip of the pipetter due to the surface tension, are separated from the tip of the tip by the force of blowing the gas or the suction force of the gas flow and are supplied to the reaction vessel. As a result, it is possible to reliably supply a certain amount of liquid to the reaction container without the liquid droplets remaining on the tip of the pipetter and not being supplied to the reaction container as in the conventional case.
【００１３】また、上記のようにしてピペッター先端の
チップに残る液滴を反応容器に供給させるようにする
と、従来のように、チップに付着した液滴を反応容器内
における液体の液面に接触させたり、反応容器の内壁面
に接触させたりするという困難な操作を行う必要がなく
なり、一定量の液体を簡単に反応容器に分注させること
ができ、特に、分注させる液体の量が１μｌ以下の微量
な量であっても、この液体を確実に分注させて検査する
ことができるようになると共に、このような分注操作も
自動化できるようになる。Further, when the droplets remaining on the tip of the pipettor are supplied to the reaction vessel as described above, the droplets attached to the tip are brought into contact with the liquid surface of the liquid in the reaction vessel as in the conventional case. It is no longer necessary to perform a difficult operation of contacting the inner wall surface of the reaction container or contacting the inner wall surface of the reaction container, and a fixed amount of liquid can be easily dispensed into the reaction container. In particular, the amount of the liquid to be dispensed is 1 μl. Even with the following minute amount, the liquid can be surely dispensed and inspected, and such dispensing operation can be automated.
【００１４】[0014]
【実施例】以下、この発明の実施例に係る分注装置を添
付図面に基づいて具体的に説明する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A dispensing device according to embodiments of the present invention will be specifically described below with reference to the accompanying drawings.
【００１５】この実施例における分注装置においては、
図５に示すように、ピペッター２０の先端におけるチッ
プ２１の周囲に気体を吹き付ける気体噴出手段４０とし
て、上記チップ２１の外径より大きな内径を有する筒状
になった気体案内部４１の外周に気体供給管４２を取り
付けたものを用い、上記の気体供給管４２から気体案内
部４１内に気体を供給するようにした。In the dispensing device of this embodiment,
As shown in FIG. 5, the gas jetting means 40 for blowing gas around the tip 21 at the tip of the pipettor 20 serves as a gas guide portion 41 having a cylindrical inner diameter larger than the outer diameter of the tip 21. A gas supply pipe 42 was used, and gas was supplied from the gas supply pipe 42 into the gas guide portion 41.
【００１６】そして、このような気体噴出手段４０によ
ってピペッター２０の先端におけるチップ２１の周囲に
気体を吹き付けるようにするにあたっては、上記の筒状
になった気体案内部４１内にピペッター２０先端のチッ
プ２１を挿入させ、この気体案内部４１の内壁面とチッ
プ２１の外周面との間に隙間が生じるようにして、上記
の気体噴出手段４０をピペッター２０の先端に上下動可
能に取り付け、上記のように気体供給管４２から気体案
内部４１内に供給された気体がチップ２１の外周面との
間の隙間を通して流れて、チップ２１の周囲に吹き付け
られるようにした。In order to blow the gas around the tip 21 at the tip of the pipetter 20 by means of the gas ejecting means 40, the tip of the tip of the pipetter 20 is inserted into the tubular gas guide portion 41. 21 is inserted so that a gap is formed between the inner wall surface of the gas guide portion 41 and the outer peripheral surface of the tip 21, and the gas ejection means 40 is attached to the tip of the pipetter 20 so as to be vertically movable. As described above, the gas supplied from the gas supply pipe 42 into the gas guide portion 41 flows through the gap between the tip 21 and the outer peripheral surface thereof and is blown around the tip 21.
【００１７】ここで、上記のように気体案内部４１をピ
ペッター２０の先端に取り付ける場合、チップ２１に付
着した検査液１１の液滴がチップ２１の周囲に吹き付け
られる気体によってうまくチップ２１から離脱されるよ
うにするため、上記のようにしてチップ２１の周囲に気
体を吹き付ける状態では、上記の気体案内部４１の下端
がチップ２１の先端と同じ位置、或はチップ２１の先端
より若干下方に突出するようにし、通常は、気体案内部
４１の下端がチップ２１の先端より０〜１０ｍｍ程度下
方に突出するようにした。Here, when the gas guide portion 41 is attached to the tip of the pipetter 20 as described above, the droplets of the test liquid 11 adhering to the tip 21 are successfully separated from the tip 21 by the gas blown around the tip 21. For this reason, in the state where the gas is blown around the tip 21 as described above, the lower end of the gas guide portion 41 projects at the same position as the tip of the tip 21 or slightly below the tip of the tip 21. Normally, the lower end of the gas guide portion 41 is made to project downward about 0 to 10 mm from the tip of the tip 21.
【００１８】次に、この実施例の分注装置を用いて、試
料容器１０内に収容された検査液１１を採取し、このよ
うに採取した検査液１１を分注用プレート等の反応容器
３０において被検査液３１が収容された底部が半球状に
なった各収容部３２に分注させる場合について具体的に
説明する。Next, the test solution 11 contained in the sample container 10 is sampled using the dispensing apparatus of this embodiment, and the test solution 11 sampled in this manner is used as a reaction container 30 such as a dispensing plate. A specific description will be given of the case where the test liquid 31 is dispensed into each of the storage parts 32 in which the bottom part has a hemispherical shape.
【００１９】先ず、試料容器１０内に収容された検査液
１１を採取するにあたっては、図６に示すように、ピペ
ッター２０の先端に取り付けられた上記の気体案内部４
１を上方に移動させて、ピペッター２０先端のチップ２
１を気体案内部４１より下方に突出させ、この状態でピ
ペッター２０先端のチップ２１を試料容器１０に収容さ
れた検査液１１中に挿入し、ピペッター２０により所定
量の検査液１１を採取するようにした。First, when the test liquid 11 contained in the sample container 10 is sampled, as shown in FIG. 6, the above-mentioned gas guide portion 4 attached to the tip of the pipetter 20.
1 and move it upward, and tip 2 of the pipettor 20
1 is projected downward from the gas guide portion 41, and in this state, the tip 21 of the tip of the pipetter 20 is inserted into the test solution 11 contained in the sample container 10, and a predetermined amount of test solution 11 is collected by the pipettor 20. I chose
【００２０】このようにしてピペッター２０により所定
量の検査液１１を採取した後は、ピペッター２０を試料
容器１０内から取り出し、上記のように上方に移動され
た状態にある気体案内部４１を下方に移動させると共
に、このピペッター２０を反応容器３０側に移動させる
ようにした。After the predetermined amount of the test liquid 11 is collected by the pipettor 20 in this way, the pipetter 20 is taken out of the sample container 10 and the gas guide portion 41 in the state moved upward as described above is moved downward. And the pipettor 20 was moved to the reaction container 30 side.
【００２１】そして、上記のようにピペッター２０によ
って採取した検査液１１を反応容器３０の各収容部３２
に分注させるにあたっては、図７に示すように、気体案
内部４１が取り付けられたピペッター２０先端のチップ
２１を被検査液１１が収容された反応容器３０の収容部
３２内に導き、この状態でチップ２１から一定量の検査
液１１を収容部３２内に供給すると共に、上記の気体供
給管４２を通して気体を気体案内部４１内に供給し、こ
のように供給された気体をこの気体案内部４１を通して
チップ２１の周囲に吹き付けるようにした。Then, the test solution 11 collected by the pipettor 20 as described above is used to store the test solution 11 in each of the accommodating portions 32 of the reaction container 30.
7, the tip 21 of the tip of the pipettor 20 to which the gas guide portion 41 is attached is introduced into the accommodating portion 32 of the reaction container 30 in which the liquid 11 to be inspected is accommodated, as shown in FIG. In addition to supplying a fixed amount of the test liquid 11 from the chip 21 into the accommodating part 32, the gas is supplied into the gas guiding part 41 through the gas supply pipe 42, and the gas thus supplied is supplied to the gas guiding part. It was made to spray around the chip 21 through 41.
【００２２】このようにして気体をチップ２１の周囲に
吹き付けると、チップ２１に付着する検査液１１の液滴
が、この気体の吹き付け力や気体の流れによる吸引力に
よってチップ２１から離脱して収容部３２に供給される
ようになった。When the gas is sprayed around the chip 21 in this way, the liquid droplets of the test liquid 11 adhering to the chip 21 are separated from the chip 21 by the spraying force of the gas or the suction force of the flow of the gas and stored. Now it is supplied to the part 32.
【００２３】このため、この実施例の分注装置を用いる
と、ピペッター２０先端のチップ２１から反応容器３０
の収容部３２に検査液１１を供給する場合に、検査液１
１の液滴がチップ２１に残留するということがなく、一
定量の検査液１１が正確に収容部３２に供給され、反応
容器３０の各収容部３２に検査液１１が正確に分注され
るようになり、特に、１μｌ以下の微量の検査液１１を
反応容器３０の各収容部３２に供給して検査を行う場合
にも、この微量な検査液１１が確実に反応容器３０の各
収容部３２に供給されるようになった。Therefore, when the dispensing apparatus of this embodiment is used, the tip 21 of the pipettor 20 to the reaction container 30
When the test liquid 11 is supplied to the container 32 of the
One drop of liquid does not remain on the chip 21, a certain amount of the test liquid 11 is accurately supplied to the container 32, and the test liquid 11 is accurately dispensed to each container 32 of the reaction container 30. In particular, even when a small amount of the test liquid 11 of 1 μl or less is supplied to each container 32 of the reaction container 30 to perform an inspection, the minute amount of the test liquid 11 is surely contained in each container of the reaction container 30. It has been supplied to 32.
【００２４】また、この実施例の分注装置を用いた場
合、チップ２１に付着した検査液１１の液滴を反応容器
３０における収容部３２に供給するために、従来のよう
に、チップ２１に付着した検査液１１の液滴を収容部３
２に収容された被検査液３１の液面に接触させたり、収
容部３２の内壁面３２ａに接触させたりするという困難
な作業を行う必要がなく、チップ２１に付着した検査液
１１の液滴を簡単に収容部３２に供給させることがで
き、上記のような分注操作の自動化も簡単に行えるよう
になった。When the dispenser of this embodiment is used, in order to supply the liquid droplets of the test liquid 11 adhering to the chip 21 to the accommodating portion 32 of the reaction container 30, the chip 21 is supplied to the chip 21 as in the conventional case. The accommodating portion 3 stores the adhered droplets of the test liquid 11.
It is not necessary to perform the difficult work of bringing into contact with the liquid surface of the test liquid 31 stored in 2 or the inner wall surface 32a of the storage portion 32, and the droplet of the test liquid 11 attached to the chip 21. Can be easily supplied to the container 32, and the dispensing operation as described above can be easily automated.
【００２５】なお、この実施例における分注装置におい
ては、採取した検査液１１を反応容器３０の収容部３２
に供給する時に、気体噴出手段４０によってチップ２１
の周囲に気体を吹き付けるようにするだけでよいが、こ
のように検査液１１を反応容器３０の収容部３２に供給
する時にだけ気体の吹き付けを行うように気体噴出手段
４０を制御することが面倒である場合には、気体噴出手
段４０から常時気体を吹き付けるようにしてもよい。但
し、このように常時気体をチップ２１の周囲に吹き付け
る場合、吹き付ける気体の圧力が高すぎると、採取した
検査液１１が反応容器３０の収容部３２に供給される前
に、この気体の吹き付けによって飛び出したりするおそ
れがあるため、吹き付ける気体の圧力を調整する必要が
あり、例えば、気体案内部４１の内壁面とチップ２１の
外周面との間における隙間が０．５〜１．５ｍｍである
場合には、気体供給管４２から供給する気体の圧力を５
００〜５０００ｍｍＨｇ程度に、また上記の隙間が１．
５〜３．０ｍｍである場合には、気体供給管４２から供
給する気体の圧力を５０００〜１００００ｍｍＨｇ程度
にすることが好ましい。In the dispensing apparatus of this embodiment, the sampled test liquid 11 is stored in the container 32 of the reaction container 30.
When the gas is supplied to the chip 21 by the gas jetting means 40.
However, it is troublesome to control the gas ejection means 40 so that the gas is blown only when the test liquid 11 is supplied to the accommodation portion 32 of the reaction container 30 in this manner. In this case, the gas may be constantly blown from the gas jetting means 40. However, in the case where the gas is constantly blown around the tip 21 as described above, if the pressure of the blown gas is too high, before the collected test liquid 11 is supplied to the accommodating portion 32 of the reaction container 30, the gas is blown by the blown gas. Since there is a risk of popping out, it is necessary to adjust the pressure of the gas to be blown. For example, when the gap between the inner wall surface of the gas guide portion 41 and the outer peripheral surface of the chip 21 is 0.5 to 1.5 mm. The pressure of the gas supplied from the gas supply pipe 42 to 5
About 0.00 to 5000 mmHg, and the above gap is 1.
In the case of 5 to 3.0 mm, the pressure of the gas supplied from the gas supply pipe 42 is preferably set to about 5000 to 10000 mmHg.
【００２６】また、この実施例における分注装置におい
ては、チップ２１の周囲に気体を吹き付ける気体噴出手
段４０をチップ２１に取り付けるようにしたが、気体噴
出手段４０を設ける方法は、特にこのようなものに限定
されるものではない。Further, in the dispensing apparatus of this embodiment, the gas jetting means 40 for blowing the gas around the tip 21 is attached to the tip 21, but the method of providing the gas jetting means 40 is particularly such a method. It is not limited to one.
【００２７】例えば、図８の（Ａ），（Ｂ）に示すよう
に、中空状になった気体案内部４１に気体を供給する気
体供給管４２が取り付けられた気体噴出手段４０を、ピ
ペッター２０に取り付けずに、被検査液１１が収容され
た反応容器３０における収容部３２の上方に設けるよう
にしてもよい。For example, as shown in FIGS. 8 (A) and 8 (B), the pipette 20 is provided with a gas jetting means 40 having a gas supply pipe 42 for supplying gas to a hollow gas guide portion 41. Instead of attaching the liquid to be inspected 11 above, it may be provided above the containing portion 32 in the reaction container 30 containing the liquid to be inspected 11.
【００２８】そして、このように収容部３２の上方に気
体噴出手段４０を設けた場合には、ピペッター２０によ
って試料容器１０から検査液１１を採取した後、このピ
ペッター２０先端のチップ２１を気体案内部４１上面の
開口部４１ａからこの気体案内部４１内に挿入し、この
状態でピペッター２０先端のチップ２１から一定量の検
査液１１を収容部３２に供給すると共に、上記の気体供
給管４２を通して気体を気体案内部４１内に供給し、こ
のように供給された気体をこの気体案内部４１を通して
チップ２１の周囲に吹き付けるようにする。When the gas jetting means 40 is provided above the accommodating portion 32 in this way, after the test liquid 11 is sampled from the sample container 10 by the pipettor 20, the tip 21 at the tip of the pipetter 20 is guided by gas. It is inserted into the gas guide portion 41 from the opening 41a on the upper surface of the portion 41, and in this state, a certain amount of the test liquid 11 is supplied from the tip 21 of the tip of the pipetter 20 to the housing portion 32, and through the gas supply pipe 42 described above. The gas is supplied into the gas guide portion 41, and the gas thus supplied is blown around the tip 21 through the gas guide portion 41.
【００２９】なお、このようにしてチップ２１の周囲に
気体を吹き付けた場合にも、上記実施例の場合と同様
に、チップ２１の先端に付着する検査液１１の液滴が気
体の吹き付け力や気体の流れによる吸引力によってチッ
プ２１から離脱して反応容器３０の収容部３２内に供給
され、一定量の検査液１１が収容部３２に正確且つ確実
に供給されるようになり、上記実施例の場合と同様の効
果が得られた。Even when the gas is blown around the tip 21 in this manner, the liquid droplets of the test liquid 11 attached to the tip of the tip 21 have the same gas blowing force as the case of the above embodiment. The suction force generated by the flow of the gas separates the chip 21 from the chip 21 and supplies the test liquid 11 into the accommodation portion 32 of the reaction container 30 so that a fixed amount of the test liquid 11 is accurately and reliably supplied to the accommodation portion 32. The same effect as in the case of was obtained.
【００３０】[0030]
【発明の効果】以上詳述したように、この発明における
分注装置においては、ピペッターによって採取した液体
をこのピペッター先端のチップから反応容器に供給する
にあたり、気体噴出手段からこのピペッター先端におけ
るチップの周囲に気体を吹き付け、この気体の吹き付け
によりチップの先端に付着する液滴をチップから離脱さ
せて反応容器に供給するようにしたため、ピペッター先
端のチップに上記液体の液滴が残留するということがな
く、一定量の液体がピペッター先端のチップから反応容
器に正確に供給されて、正確な分注が行えるようにな
り、特に、分注させる液体の量が１μｌ以下の微量な量
であっても、この液体を正確且つ確実に反応容器に分注
させることができるようになり、反応容器に収容された
被検査試料等の検査が正確に行えるようになった。As described in detail above, in the dispensing apparatus of the present invention, when the liquid collected by the pipettor is supplied to the reaction vessel from the tip of the pipettor, the tip of the tip of the pipetter is discharged from the gas jetting means. Since a gas was blown around and the droplets adhering to the tip of the tip were separated from the tip and supplied to the reaction vessel by the blowing of the gas, it is possible that the droplet of the liquid remains on the tip of the pipettor tip. Instead, a fixed amount of liquid is accurately supplied from the tip of the pipettor to the reaction vessel, and accurate dispensing becomes possible. Especially, even when the amount of liquid to be dispensed is a minute amount of 1 μl or less. It becomes possible to dispense this liquid accurately and surely into the reaction container, and to inspect the sample to be inspected stored in the reaction container. I was able to perform accurately.
【００３１】また、この発明における分注装置において
は、上記のようにピペッター先端のチップの周囲に気体
噴出手段から気体を吹き付け、チップの先端に付着する
液体の液滴をチップから離脱させて反応容器に供給する
ようにしたため、従来のようにチップの先端に付着した
液体の液滴を反応容器に収容された被検査試料等の液体
の液面に接触させたり、反応容器の内壁面に接触させた
りするという困難な作業を行わなくとも、一定量の液体
を正確且つ確実に反応容器に分注させることが簡単に行
えるようになり、分注操作の自動化も簡単に行えるよう
になった。Further, in the dispensing apparatus of the present invention, as described above, gas is blown from the gas jetting means around the tip of the pipettor to cause the liquid droplets adhering to the tip of the tip to separate from the tip and react. Since it is supplied to the container, the liquid droplets attached to the tip of the chip are brought into contact with the liquid surface of the liquid such as the sample to be inspected stored in the reaction container or the inner wall surface of the reaction container as in the conventional case. It has become possible to easily and accurately dispense a fixed amount of liquid into a reaction container without performing a difficult work such as performing the operation, and automation of the dispensing operation has become easy.
【図１】ピペッターによって検査液を試料容器から採取
する状態及び採取した検査液を被検査液が収容された反
応容器の収容部に分注させる状態を示した概略断面図で
ある。FIG. 1 is a schematic cross-sectional view showing a state in which a test liquid is sampled from a sample container by a pipettor and a state in which the sampled test liquid is dispensed into an accommodation part of a reaction container accommodating a liquid under test.
【図２】ピペッター先端のチップに検査液の液滴が付着
した状態を示した概略断面図である。FIG. 2 is a schematic cross-sectional view showing a state in which a droplet of a test liquid is attached to the tip of the pipettor.
【図３】ピペッターのチップに付着した検査液の液滴を
反応容器の収容部に収容された被検査液の液面に接触さ
せて収容部に供給する状態を示した断面説明図である。FIG. 3 is a cross-sectional explanatory view showing a state in which droplets of the test liquid adhering to the tip of the pipettor are brought into contact with the liquid surface of the test liquid contained in the container of the reaction container and supplied to the container.
【図４】ピペッターのチップに付着した検査液の液滴を
反応容器の収容部の内壁面に接触させて収容部に供給す
る状態を示した断面説明図である。FIG. 4 is a cross-sectional explanatory view showing a state in which droplets of the test liquid adhering to the tip of the pipettor are brought into contact with the inner wall surface of the accommodation portion of the reaction container and supplied to the accommodation portion.
【図５】この発明の実施例に係る分注装置において、気
体噴出手段をピペッターの先端に取り付けた状態を示し
た断面説明図である。FIG. 5 is a cross-sectional explanatory view showing a state in which the gas ejection means is attached to the tip of the pipettor in the dispensing apparatus according to the embodiment of the present invention.
【図６】同実施例の分注装置において、試料容器に収容
された検査液をピペッターによって採取する状態を示し
た断面説明図である。FIG. 6 is a cross-sectional explanatory view showing a state in which the test liquid contained in the sample container is collected by a pipetter in the dispensing apparatus of the embodiment.
【図７】同実施例の分注装置において、採取した検査液
をピペッターのチップから反応容器の収容部に供給する
にあたり、チップ先端に付着した検査液の液滴を気体噴
出手段によって収容部に供給する状態を示した断面説明
図である。FIG. 7 is a view showing a dispenser of the same embodiment, in which the collected test liquid is supplied from the tip of the pipettor to the accommodating portion of the reaction container, and the liquid droplet of the test liquid adhering to the tip of the tip is admitted to the accommodating portion by the gas ejection means. It is sectional explanatory drawing which showed the state supplied.
【図８】この発明の実施例に係る分注装置において、気
体噴出手段を設ける状態の変更例を示し、採取した検査
液をピペッター先端のチップから反応容器の収容部に供
給する状態を示した断面説明図である。FIG. 8 shows a modified example of the state in which the gas jetting means is provided in the dispensing apparatus according to the embodiment of the present invention, and shows the state in which the collected test liquid is supplied from the tip of the pipettor to the accommodating portion of the reaction container. FIG.
１１ 検査液（液体） ２０ ピペッター ２１ チップ ３０ 反応容器 ３１ 被検査液 ４０ 気体噴出手段 ４１ 気体案内部 ４２ 気体供給管 11 Test Liquid (Liquid) 20 Pipettor 21 Chip 30 Reaction Vessel 31 Test Liquid 40 Gas Ejection Means 41 Gas Guide 42 Gas Supply Pipe
Claims (1)
ピペッター先端のチップから反応容器に供給して分注を
行う分注装置において、採取した液体をピペッター先端
のチップから反応容器に供給する際に、このチップの周
囲に気体を吹き付ける気体噴出手段を設けたことを特徴
とする分注装置。1. A dispenser for dispensing a liquid collected by a pipetter from a tip of the pipettor to a reaction container to dispense the collected liquid to a reaction container from the tip of the pipettor. A dispensing device characterized in that a gas jetting means for blowing a gas around the tip is provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6076699A JPH07260799A (en) | 1994-03-22 | 1994-03-22 | Distribution device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6076699A JPH07260799A (en) | 1994-03-22 | 1994-03-22 | Distribution device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07260799A true JPH07260799A (en) | 1995-10-13 |
Family
ID=13612757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6076699A Pending JPH07260799A (en) | 1994-03-22 | 1994-03-22 | Distribution device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07260799A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008542689A (en) * | 2005-05-06 | 2008-11-27 | インストゥルメンテーション ラボラトリー カンパニー | Nested closed tube sampling assembly |
| JP2009530594A (en) * | 2006-03-13 | 2009-08-27 | ビオメリュー | Apparatus, use and method for removing liquid |
| JP2012533082A (en) * | 2009-10-29 | 2012-12-20 | ポステック アカデミー−インダストリー ファンデーション | Droplet discharge device |
| EP3321689A1 (en) * | 2016-11-09 | 2018-05-16 | Siemens Healthcare Diagnostics Products GmbH | System for pipetting liquids in an automatic analyzer |
-
1994
- 1994-03-22 JP JP6076699A patent/JPH07260799A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008542689A (en) * | 2005-05-06 | 2008-11-27 | インストゥルメンテーション ラボラトリー カンパニー | Nested closed tube sampling assembly |
| JP2013064751A (en) * | 2005-05-06 | 2013-04-11 | Instrumentation Laboratory Co | Nested closed tube sampling assembly |
| JP2009530594A (en) * | 2006-03-13 | 2009-08-27 | ビオメリュー | Apparatus, use and method for removing liquid |
| JP4896162B2 (en) * | 2006-03-13 | 2012-03-14 | ビオメリュー | Apparatus, use and method for removing liquid |
| JP2012533082A (en) * | 2009-10-29 | 2012-12-20 | ポステック アカデミー−インダストリー ファンデーション | Droplet discharge device |
| US8475741B2 (en) | 2009-10-29 | 2013-07-02 | Postech Academy-Industry Foundation | Droplet discharging device |
| EP3321689A1 (en) * | 2016-11-09 | 2018-05-16 | Siemens Healthcare Diagnostics Products GmbH | System for pipetting liquids in an automatic analyzer |
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