JPH0666779A - Method for filling up liquid chromatographic column with slurry with pressurized gas - Google Patents
Method for filling up liquid chromatographic column with slurry with pressurized gasInfo
- Publication number
- JPH0666779A JPH0666779A JP4242543A JP24254392A JPH0666779A JP H0666779 A JPH0666779 A JP H0666779A JP 4242543 A JP4242543 A JP 4242543A JP 24254392 A JP24254392 A JP 24254392A JP H0666779 A JPH0666779 A JP H0666779A
- Authority
- JP
- Japan
- Prior art keywords
- column
- solvent
- slurry
- filling
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
- G01N2030/562—Packing methods or coating methods packing
- G01N2030/565—Packing methods or coating methods packing slurry packing
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明は、充填剤スラリーを、
ガス、例えば窒素ガスの圧力で加圧して液体クロマトカ
ラムに充填する方法に関する。This invention relates to a filler slurry,
The present invention relates to a method for filling a liquid chromatographic column by pressurizing with a gas such as nitrogen gas.
【０００２】[0002]
【従来の技術】液体クロマトカラムに充填する充填剤ス
ラリーの溶媒には、通常、可燃性の液体が使用される。
このため、充填の際には、クロマトカラムに溶媒を満た
し、カラムの下端と密閉の溶媒受けタンクとの間を排出
管で接続し、溶媒受けタンク中に０．５ｋｇ／ｃｍ2 ・
Ｇ以下の低圧の窒素ガスを導入してシールを行い、空気
を遮断して爆発の危険を予防してから、充填剤スラリー
を容れたスラリータンク中に１．０ｋｇ／ｃｍ2 ・Ｇ以
上の高圧の窒素ガスを導入し、スラリータンクと液体ク
ロマトカラムの上端の充填用上蓋とをつなぐ供給管を通
じカラム内に充填剤スラリーを充填し、カラム中に予め
満たした溶媒は溶媒受けタンクに押出す。2. Description of the Related Art A flammable liquid is usually used as a solvent for a packing material slurry packed in a liquid chromatography column.
Therefore, at the time of filling, the chromatographic column is filled with a solvent, the lower end of the column and the closed solvent receiving tank are connected by a discharge pipe, and 0.5 kg / cm 2 ·
Introduce a low pressure nitrogen gas of G or less to seal and shut off the air to prevent the danger of explosion, and then add a high pressure of 1.0 kg / cm 2 · G or more in the slurry tank containing the filler slurry. The nitrogen gas is introduced into the column, and the packing slurry is filled in the column through a supply pipe connecting the slurry tank and the upper lid for packing at the upper end of the liquid chromatography column, and the solvent previously filled in the column is extruded into the solvent receiving tank.
【０００３】[0003]
【発明が解決しようとする課題】スラリーの充填が終わ
ると、それまでカラムの上端に取り付けてあった充填用
の上蓋を外し、通液用の上蓋に付け換える。このとき、
カラムの内部は大気圧に戻るので、充填の際に充填剤ス
ラリーを加圧して充填剤スラリーの溶媒中に溶解したガ
スが気泡となって該スラリーの溶媒から出、溶媒は激し
く泡立つバブリング現象を起こし、このため充填剤層は
乱され、性能の低いカラムしか作製することができな
い。尚、大型のカラムの場合は充填用の上蓋と、通液用
の上蓋を付け換える作業が大変なので、通液用の上蓋を
常時、取付け、スラリー充填の際はカラムの上部にカラ
ム内に連通する着脱可能なパイプを取付け、スラリータ
ンクからの供給管の下端をこのパイプに接続してスラリ
ーをカラムに充填するが、充填後にパイプをカラムから
取外す際にカラムの内部は大気に開放するので、同様に
カラム内の充填剤層は激しくバブリングし、性能を低下
する。When the filling of the slurry is completed, the upper lid for packing, which has been attached to the upper end of the column until then, is removed and replaced with the upper lid for liquid passage. At this time,
Since the inside of the column returns to atmospheric pressure, the filler slurry is pressurized during filling and the gas dissolved in the solvent of the filler slurry becomes bubbles and exits from the solvent of the slurry, causing the solvent to bubbly bubble. And thus the packing material layer is disturbed and only columns with poor performance can be produced. In the case of a large column, it is difficult to replace the upper lid for packing with the upper lid for liquid passage.Therefore, always attach the upper lid for liquid passage, and connect the upper lid of the column to the inside of the column when filling the slurry. Attach a removable pipe to connect the lower end of the supply pipe from the slurry tank to this pipe to fill the column with slurry, but when removing the pipe from the column after filling, the inside of the column is open to the atmosphere, Similarly, the packing layer in the column will bubbling violently, degrading performance.
【０００４】[0004]
【課題を解決するための手段】本発明は上記問題を解消
し、高性能なカラムを作製することを目的に開発された
のであって、液体クロマトカラム内へガスで加圧して充
填剤スラリーを充填する液体クロマトカラムへのガス加
圧式スラリー充填方法において、充填剤スラリーを充填
し終わった時点から引き続き前記ガスをカラムに導入す
るか、又は溶媒をカラムに導入し、カラム中の充填剤ス
ラリーの溶媒をカラムの外に押出すことを特徴とする。The present invention has been developed for the purpose of solving the above problems and producing a high-performance column, and a filler slurry is obtained by pressurizing a gas into a liquid chromatographic column with a gas. In the gas pressure type slurry filling method for the liquid chromatography column to be filled, the gas is continuously introduced into the column from the time when the packing of the filler slurry is completed, or the solvent is introduced into the column, and the filler slurry in the column is It is characterized in that the solvent is extruded out of the column.
【０００５】[0005]
【実施例】図１の実施例において、１は液体クロマトカ
ラム、２は充填剤スラリーを入れたスラリータンク、３
はスラリーの供給管、４は溶媒受けタンク、５はクロマ
トカラムから溶媒受けタンクに溶媒を排出する排出管、
６はスラリータンク内の攪拌機、７はスラリータンクの
内部を洗浄する散布装置である。図１Ａはスラリータン
ク２中の充填剤スラリーを該タンクに導入した高圧の窒
素ガスでクロマトカラム１内に充填中の状態で、クロマ
トカラムの上面には充填用上蓋８を取付けてあって、供
給管３の下端は、該上蓋の充填口に接続し、溶媒受けタ
ンク４には低圧の窒素ガスを導入し、クロマトカラム１
内に予め満たした溶媒は、クロマトカラムに充填される
充填剤スラリーによって逐次、排出管５を通じ溶媒受け
タンク４に押出される。尚、スラリータンク２はクロマ
トカラム１を満たす必要量の充填剤スラリーが容れてあ
り、充填中は攪拌機６でタンク内の充填剤スラリーを攪
拌する。EXAMPLE In the example of FIG. 1, 1 is a liquid chromatographic column, 2 is a slurry tank containing a filler slurry, 3
Is a slurry supply pipe, 4 is a solvent receiving tank, 5 is a discharge pipe for discharging the solvent from the chromatographic column to the solvent receiving tank,
6 is a stirrer in the slurry tank, and 7 is a spraying device for cleaning the inside of the slurry tank. FIG. 1A shows a state in which the packing slurry in the slurry tank 2 is being packed in the chromatographic column 1 by high-pressure nitrogen gas introduced into the tank, and a packing upper lid 8 is attached to the upper surface of the chromatographic column for supply. The lower end of the pipe 3 is connected to the filling port of the upper lid, low-pressure nitrogen gas is introduced into the solvent receiving tank 4, and the chromatographic column 1
The solvent filled in advance is sequentially extruded into the solvent receiving tank 4 through the discharge pipe 5 by the filler slurry filled in the chromatographic column. The slurry tank 2 contains a required amount of filler slurry that fills the chromatographic column 1, and the filler slurry in the tank is agitated by a stirrer 6 during filling.
【０００６】図１Ｂは充填末期で、スラリータンク内の
充填剤スラリーが無くなる直前に散布装置７から溶媒を
シャワーし、タンク壁面に付着する充填剤を洗い落と
し、この充填剤も溶媒ともどもクロマトカラム１に充填
する。上記した図１Ａ、図１Ｂに示す工程は、従来のガ
ス加圧式スラリー充填方法でも行われているものであ
る。FIG. 1B shows the final stage of filling, in which the solvent is showered from the spraying device 7 immediately before the filler slurry in the slurry tank is exhausted to wash off the filler adhering to the wall surface of the tank. Fill. The above-mentioned steps shown in FIGS. 1A and 1B are also performed by the conventional gas pressure type slurry filling method.
【０００７】図１Ｃは、本発明によって行うクロマトカ
ラムからの液抜き工程であって、スラリータンク内に充
填剤スラリーが無くなっても、引き続きスラリータンク
内に高圧の窒素ガスを導入し、この窒素ガスを供給管３
からクロマトカラム１に作用させ、クロマトカラム中に
充填剤と同伴して供給された充填剤スラリーの溶媒を溶
媒受けタンク４に排出する。これによってスラリータン
クから高圧の窒素ガスで加圧して、クロマトカラムに充
填剤スラリーを充填する際に、窒素ガスが多量に溶解し
た充填剤スラリーの溶媒は溶媒受けタンクに移行し、ク
ロマトカラムの中は充填剤層１０と、充填剤スラリーの
溶媒を排出した窒素ガスだけになる。尚、溶媒受けタン
クは当初にクロマトカラムを満たしていた溶媒と、クロ
マトカラムに充填した充填剤スラリーの溶媒の双方を受
入れることができる容量のものを使用する。FIG. 1C shows a process of draining liquid from a chromatographic column according to the present invention. Even if there is no filler slurry in the slurry tank, a high-pressure nitrogen gas is continuously introduced into the slurry tank. Supply pipe 3
To act on the chromatographic column 1 to discharge the solvent of the filler slurry supplied together with the filler into the chromatographic column to the solvent receiving tank 4. As a result, when the high-pressure nitrogen gas is pressurized from the slurry tank to fill the packing slurry in the chromatographic column, the solvent of the packing slurry in which a large amount of nitrogen gas is dissolved moves to the solvent receiving tank, Is only the filler layer 10 and the nitrogen gas discharged from the solvent of the filler slurry. The solvent receiving tank has a capacity capable of receiving both the solvent initially filled in the chromatographic column and the solvent of the filler slurry filled in the chromatographic column.
【０００８】クロマトカラム中の充填剤スラリーの溶媒
が全量、溶媒受けタンクに排出され、タンクに窒素ガス
が入るようになったらスラリータンク、及び溶媒受けタ
ンクへの窒素ガスの導入を停止し、クロマトカラムを供
給管３、排出管５との接続から外すと共に、両管と接続
していた注入口、排出口を閉じ、図１Ｄに示すようにク
ロマトカラムの上面に取付けてあった充填用上蓋８を外
し、運転用の通液上蓋９を取付ける。このときクロマト
カラムの内部は大気に開放するが、カラム内部の充填剤
層１０中には溶媒である液が存在しないため、充填剤層
１０に含まれている窒素ガスはバブリング現象を生じる
ことなくカラムの外に脱気する。When all the solvent of the packing material slurry in the chromatographic column is discharged to the solvent receiving tank and nitrogen gas comes into the tank, the introduction of nitrogen gas into the slurry tank and the solvent receiving tank is stopped, The column was disconnected from the connection with the supply pipe 3 and the discharge pipe 5, the inlet and the discharge port connected to both pipes were closed, and the packing upper lid 8 attached to the upper surface of the chromatographic column as shown in FIG. 1D. And remove the liquid passage upper lid 9 for operation. At this time, the inside of the chromatographic column is opened to the atmosphere, but since the liquid that is a solvent does not exist in the packing layer 10 inside the column, the nitrogen gas contained in the packing layer 10 does not cause a bubbling phenomenon. Degas outside the column.
【０００９】クロマトカラムの充填剤層中には窒素ガス
が存在しないことが望ましいので、上蓋の交換後、図１
Ｅに示すようにカラムの下端の排出口に溶媒をポンプで
送り込む送液管１１を接続し、カラム内に上向流で溶媒
を入れ、カラム内を溶媒で満たし、充填剤層１０中に含
まれている窒素ガスを通液上蓋９の通液口から排気す
る。次に、カラム内の充填剤層を安定させるため、図１
Ｆに示すように前記送液管１１を通液上蓋の通液口に接
続し、カラム内に下向流で溶媒を流し、前工程において
上向流でカラムに満たした溶媒を下端の排出口から排出
し、溶媒受けタンク４に回収する。Since it is desirable that nitrogen gas does not exist in the packing material layer of the chromatographic column, after the replacement of the upper lid, as shown in FIG.
As shown in E, a discharge pipe at the lower end of the column is connected to a liquid feed pipe 11 for feeding the solvent by a pump, the solvent is put in the column in an upward flow, the column is filled with the solvent, and the solvent is contained in the packing material layer 10. The nitrogen gas is discharged from the liquid passage port of the liquid upper lid 9. Next, in order to stabilize the packing material layer in the column, as shown in FIG.
As shown in F, the liquid supply pipe 11 is connected to the liquid passage of the liquid upper lid, the solvent is caused to flow in the column in a downward flow, and the solvent filling the column in the previous step in the upward flow is discharged at the lower end. And is collected in the solvent receiving tank 4.
【００１０】クロマトカラムに直径６００ｍｍ、高さ１
０００ｍｍのものを使用し、充填剤として５０μｍのシ
リカゲル１２５ｋｇ、溶媒にＮ−ヘキサン／酢酸エチル
の６／４混合液４２０リットルを用いて充填剤スラリー
を調整し、図１に示す工程を行った。スラリータンクに
１．５〜２ｋｇ／ｃｍ2 ・Ｇの窒素ガスを導入した所、
約６分で充填剤スラリーをクロマトカラムに充填するこ
とができた。充填後に充填用上蓋を通液上蓋と付け換え
たがバブリング現象は起きなかった。尚、図１Ｅのガス
抜き、溶媒充満工程、図１Ｆの安定化調整工程は溶媒を
通液線速度（ＬＶ）＝２ｍ／Ｈｒで、３０分間行った。The chromatographic column has a diameter of 600 mm and a height of 1
The filler slurry was prepared by using a filler having a diameter of 000 mm and using 125 kg of 50 μm silica gel as a filler and 420 liters of a 6/4 mixed solution of N-hexane / ethyl acetate as a solvent, and the step shown in FIG. 1 was performed. When 1.5 to 2 kg / cm 2 · G of nitrogen gas was introduced into the slurry tank,
The chromatographic column could be filled with the filler slurry in about 6 minutes. After the filling, the filling upper lid was replaced with the liquid-passing upper lid, but the bubbling phenomenon did not occur. The degassing step, the solvent filling step, and the stabilization adjusting step shown in FIG. 1F in FIG. 1E were performed for 30 minutes at the solvent linear flow rate (LV) = 2 m / Hr.
【００１１】図１に示した実施例では、カラム内に充填
した充填剤スラリーの、窒素ガスが溶解している溶媒を
カラムから排出するために充填用の窒素ガスを使用した
が、図２に示すように窒素ガスと接触していない溶媒を
用いて排出することもできる。それには、スラリータン
クからの供給管３の途中に溶媒槽１２中の溶媒をポンプ
で送液する注液管１３を接続し、供給管と注液管には夫
々接続位置の上流に開閉弁Ｖ1 ，Ｖ2 を設け、又、溶媒
受けタンクへの排出管５の途中には背圧弁１４を接続す
る。高圧窒素ガスをスラリータンク１に導入し、タンク
中の充填剤スラリーをクロマトカラムに充填するときに
は供給管３の開閉弁Ｖ1 は開、注液管１３の開閉弁Ｖ2
は閉にしておく。そして、散布装置７によるスラリータ
ンク内のシャワー洗浄が終了し、スラリータンク中の充
填剤スラリーの全量がクロマトカラムに充填されて窒素
ガスがクロマトカラムに入る直前に供給管３の開閉弁Ｖ
1 を閉、注液管１３の開閉弁Ｖ2を開にし、ポンプを運
転して注液管１３からクロマトカラム１に溶媒を供給
し、窒素ガスが多量溶解したクロマトカラム中の充填剤
スラリーの溶媒を溶媒受けタンク４に排出する。このと
き、弁Ｖ1 を閉、Ｖ2 を開にする間も背圧弁１４の作用
によってガス加圧充填時と同程度の圧力がクロマトカラ
ムに加わるようになっている。これによって、クロマト
カラム中の、窒素ガスが多量に溶解した溶媒は溶媒受け
タンクに押し出され、クロマトカラム中の液はガスが過
剰に溶解していない溶媒と置き替わるので、クロマトカ
ラムを大気に開放してもガスの悪影響は回避できる。
尚、この場合は上蓋の付け換え後に安定化調整工程を行
うのがのぞましい。In the embodiment shown in FIG. 1, the nitrogen gas for filling was used to discharge the solvent of the filler slurry filled in the column in which the nitrogen gas is dissolved from the column. It can also be discharged using a solvent that is not in contact with nitrogen gas as shown. To this, a liquid injection pipe 13 for pumping the solvent in the solvent tank 12 is connected in the middle of the supply pipe 3 from the slurry tank, and the supply pipe and the liquid injection pipe are each provided with an opening / closing valve V upstream of the connection position. 1 , V 2 are provided, and a back pressure valve 14 is connected in the middle of the discharge pipe 5 to the solvent receiving tank. When high-pressure nitrogen gas is introduced into the slurry tank 1 and the packing slurry in the tank is filled in the chromatographic column, the open / close valve V 1 of the supply pipe 3 is opened and the open / close valve V 2 of the liquid injection pipe 13 is opened.
Is closed. Then, the shower cleaning in the slurry tank by the spraying device 7 is completed, the entire amount of the filler slurry in the slurry tank is filled in the chromatographic column, and the opening / closing valve V of the supply pipe 3 immediately before the nitrogen gas enters the chromatographic column.
1 is closed, the opening / closing valve V 2 of the liquid injection pipe 13 is opened, the pump is operated to supply the solvent from the liquid injection pipe 13 to the chromatographic column 1, and the packing slurry in the chromatographic column in which a large amount of nitrogen gas is dissolved The solvent is discharged to the solvent receiving tank 4. At this time, even while the valve V 1 is closed and V 2 is opened, the back pressure valve 14 acts to apply the same pressure to the chromatographic column as when the gas is pressurized and filled. As a result, the solvent in the chromatographic column in which a large amount of nitrogen gas is dissolved is pushed out to the solvent receiving tank, and the liquid in the chromatographic column replaces the solvent in which the gas is not excessively dissolved, so the chromatographic column is opened to the atmosphere. However, the adverse effect of gas can be avoided.
In this case, it is desirable to perform the stabilization adjustment process after replacing the upper lid.
【００１２】[0012]
【発明の効果】以上で明らかなように、本発明によれ
ば、ガスで加圧して充填剤スラリーを液体クロマトカラ
ムに充填後、クロマトカラムを大気に開放する前に、充
填剤スラリーのガスが多量に溶解した溶媒を、ガス、又
はガスと接触していない溶媒によってカラムの外に押し
出し、クロマトカラムの内部を充填剤層と、ガス、又は
ガスが殆ど溶解していない溶媒にするので、充填用上蓋
と外して通液上蓋に付け換えるなどクロマトカラムを大
気に開放しても、ガスが多量に溶解した溶媒が存在する
場合のバブリング現象は生ぜず、このため充填剤層は乱
されない。従って、従来はバブリングによって理論段数
が約１０００段のカラムしか作製できなかったが、本発
明によれば理論段数が約４７００段もの高性能なカラム
を作製することができる。As is apparent from the above, according to the present invention, the gas of the filler slurry is filled with the gas by pressurizing the gas with the gas before filling the liquid chromatographic column with the packing slurry and before opening the chromatographic column to the atmosphere. A large amount of dissolved solvent is pushed out of the column by the gas or the solvent that is not in contact with the gas, and the inside of the chromatographic column is used as the packing material layer and the gas or the solvent in which the gas is hardly dissolved. Even if the chromatographic column is opened to the atmosphere by removing it from the upper lid for use and replacing it with a liquid-passing upper lid, the bubbling phenomenon in the presence of a solvent in which a large amount of gas is dissolved does not occur, and therefore the packing layer is not disturbed. Therefore, conventionally, only a column having a theoretical plate number of about 1000 could be produced by bubbling, but according to the present invention, a high-performance column having a theoretical plate number of about 4700 can be produced.
【図１】本発明の一実施例を工程順に示す説明図であ
る。FIG. 1 is an explanatory view showing an embodiment of the present invention in process order.
【図２】本発明の他の一実施例の説明図である。FIG. 2 is an explanatory diagram of another embodiment of the present invention.
１ 液体クロマトカラム ２ スラリータンク ３ 供給管 ４ 溶媒受けタンク ５ 排出管 ８ 充填用上蓋 ９ 通液用上蓋 １０ 充填剤層 1 Liquid Chromatographic Column 2 Slurry Tank 3 Supply Pipe 4 Solvent Receiving Tank 5 Discharge Pipe 8 Upper Lid 9 for Filling 9 Upper Lid 10 for Liquid Filling
Claims (1)
充填剤スラリーを充填する液体クロマトカラムへのガス
加圧式スラリー充填方法において、充填剤スラリーを充
填し終わった時点から引き続き前記ガスをカラムに導入
するか、又は溶媒をカラムに導入し、カラム中の充填剤
スラリーの溶媒をカラムの外に押出すことを特徴とする
液体クロマトカラムへのガス加圧式スラリー充填方法。1. A method for filling a gas chromatographic slurry into a liquid chromatographic column, which comprises pressurizing a gas into a liquid chromatographic column with a filler slurry, wherein the gas is continuously introduced into the column from the time when the filler slurry is completely filled. A gas pressure type slurry packing method for a liquid chromatographic column, which comprises introducing the solvent or introducing the solvent into the column and extruding the solvent of the filler slurry in the column out of the column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4242543A JPH0666779A (en) | 1992-08-20 | 1992-08-20 | Method for filling up liquid chromatographic column with slurry with pressurized gas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4242543A JPH0666779A (en) | 1992-08-20 | 1992-08-20 | Method for filling up liquid chromatographic column with slurry with pressurized gas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0666779A true JPH0666779A (en) | 1994-03-11 |
Family
ID=17090678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4242543A Pending JPH0666779A (en) | 1992-08-20 | 1992-08-20 | Method for filling up liquid chromatographic column with slurry with pressurized gas |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0666779A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101017572B1 (en) * | 2010-01-11 | 2011-02-28 | (주) 영진바이오크롬 | Packing method of prep hplc column |
| JP2011525988A (en) * | 2008-06-27 | 2011-09-29 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Separation medium slurry tank |
| JP2016533479A (en) * | 2013-09-30 | 2016-10-27 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Slurry transfer method |
| CN117542755A (en) * | 2023-11-13 | 2024-02-09 | 苏州恩腾半导体科技有限公司 | System and method for providing heated etching solution |
-
1992
- 1992-08-20 JP JP4242543A patent/JPH0666779A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011525988A (en) * | 2008-06-27 | 2011-09-29 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Separation medium slurry tank |
| KR101017572B1 (en) * | 2010-01-11 | 2011-02-28 | (주) 영진바이오크롬 | Packing method of prep hplc column |
| JP2016533479A (en) * | 2013-09-30 | 2016-10-27 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | Slurry transfer method |
| US10195541B2 (en) | 2013-09-30 | 2019-02-05 | Ge Healthcare Bio-Sciences Ab | Method for transferring slurry |
| CN117542755A (en) * | 2023-11-13 | 2024-02-09 | 苏州恩腾半导体科技有限公司 | System and method for providing heated etching solution |
| CN117542755B (en) * | 2023-11-13 | 2024-06-07 | 苏州恩腾半导体科技有限公司 | System and method for providing heated etching solution |
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