JPH0651631B2 - Leukocyte separation material - Google Patents
Leukocyte separation materialInfo
- Publication number
- JPH0651631B2 JPH0651631B2 JP63050173A JP5017388A JPH0651631B2 JP H0651631 B2 JPH0651631 B2 JP H0651631B2 JP 63050173 A JP63050173 A JP 63050173A JP 5017388 A JP5017388 A JP 5017388A JP H0651631 B2 JPH0651631 B2 JP H0651631B2
- Authority
- JP
- Japan
- Prior art keywords
- leukocyte
- blood
- white blood
- blood cell
- bubble point
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3621—Extra-corporeal blood circuits
- A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
- A61M1/3633—Blood component filters, e.g. leukocyte filters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D39/00—Filtering material for liquid or gaseous fluids
- B01D39/14—Other self-supporting filtering material ; Other filtering material
- B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
- B01D39/1669—Cellular material
- B01D39/1676—Cellular material of synthetic origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0439—White blood cells; Leucocytes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Vascular Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Anesthesiology (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Cardiology (AREA)
- Filtering Materials (AREA)
- External Artificial Organs (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Description
【発明の詳細な説明】 （１）発明の目的 ＜産業上の利用分野＞ 本発明は、白血球分離材に関するものである。詳述する
と、本発明は、白血球に対して高く安定した捕捉能を示
しかつ異物の流出の虞れが少ない白血球分離材に関する
ものである。DETAILED DESCRIPTION OF THE INVENTION (1) Purpose of the Invention <Field of Industrial Application> The present invention relates to a leukocyte separating material. More specifically, the present invention relates to a leukocyte separating material which has a high and stable capturing ability for leukocytes and is less likely to cause foreign matter to flow out.
＜従来技術＞ 輸血の形態が従来の全血輸血から、患者が必要としてい
る成分のみを輸血する成分輸血へと変化して久しいが、
この成分輸血においては、いかに分画した血液成分の純
度を高くするかが課題となってくる。従来、献血によっ
て得られた血液は、遠心操作によって赤血球濃厚液（CR
C）、濃縮血小板血漿（PC）及び乏血小板血漿（PPP）に
分離される。このようにして分離された赤血球濃厚液
は、赤血球の成分製剤として赤血球を必要とする患者へ
の成分輸血に広く用いられいるが、赤血球濃厚液は、多
くの白血球、血小板を含み、いわゆる全成分血液である
との考えが定着しつつあり、赤血球のみを必要としてい
る患者に、赤血球濃厚液の輸血により併せて多量の白血
球及び血小板が輸血されていることが問題視されてい
る。このように赤血球濃厚液のような赤血球分画中に含
まれる白血球及び血小板は、輸血後の副作用を防止する
上からも極力除去する必要があり、このために従前より
多くの工夫がなされている。赤血球製剤の純度を高くす
る方法としては、血球の比重差を利用した重力遠心分離
方法、血球の粘着もしくは付着等を利用した捕捉材利用
の方法、赤血球凝集剤を用いた白血球分離方法等の方法
が使用されている。<Prior Art> It has been a long time since the form of blood transfusion has changed from conventional whole blood transfusion to component transfusion in which only the components required by the patient are transfused.
In this component transfusion, how to raise the purity of the fractionated blood component becomes an issue. Conventionally, blood obtained by donating blood is concentrated by red blood cell concentrate (CR
C), platelet-rich plasma (PC) and platelet-poor plasma (PPP). The erythrocyte concentrate thus separated is widely used as a component preparation of erythrocytes for transfusion of components to patients who require erythrocytes, but the erythrocyte concentrate contains many white blood cells and platelets, and so-called all components. The idea that it is blood is becoming established, and it is regarded as a problem that a large amount of white blood cells and platelets are also transfused by transfusion of concentrated red blood cells to a patient who needs only red blood cells. As described above, white blood cells and platelets contained in a red blood cell fraction such as a red blood cell concentrate need to be removed as much as possible in order to prevent side effects after blood transfusion. For this reason, many devices have been devised. . As a method for increasing the purity of a red blood cell preparation, a method such as a gravity centrifugation method using a difference in specific gravity of blood cells, a method of using a capturing material using adhesion or adhesion of blood cells, a method of separating white blood cells using a hemagglutination agent, etc. Is used.
これらの方法の中で、捕捉材利用の方法が白血球除去効
率の良さ、手技の簡便なことなどから広く用いられてい
る。捕捉材としては天然セルロース、ポリエステル、ポ
リアミド、ポリアクリロニトリル、ガラス繊維などの繊
維径の非常に小さな繊維をカラム内にそのまま詰めたも
のや不織布等に二次加工したものが多くの場合用いられ
ている。Among these methods, the method of using a capturing material is widely used because of its excellent leukocyte removal efficiency and simple procedure. As the capture material, natural cellulose, polyester, polyamide, polyacrylonitrile, glass fibers and other fibers having very small fiber diameters are packed in the column as they are, or non-woven fabrics are secondarily processed and are often used. .
＜発明が解決しようとする問題点＞ しかしながら、上述の方法において、繊維そのものをカ
ラムに詰める場合においては、繊維を均一に充填するの
が難しく製作に手間がかかるとともに、繊維の詰め方に
より操作時におけるチャネリングの発生の虞れが大き
く、さらに白血球の十分な捕捉を行うように繊維の充填
密度を高めると、濾過時間を非常に長いものとしてしま
い、さらにまた、繊維同士の絡合が十分でないために操
作中に繊維が流出してしまう虞れのあるものであった。<Problems to be Solved by the Invention> However, in the above method, when the fibers themselves are packed in the column, it is difficult to uniformly pack the fibers, it takes time to manufacture, and the fibers are packed during operation. There is a great risk of channeling in the above, and if the packing density of the fibers is increased so as to capture the white blood cells sufficiently, the filtration time becomes very long, and furthermore, the entanglement of the fibers is not sufficient. Moreover, there was a possibility that the fibers would flow out during the operation.
本発明は、上述した点に鑑み案出したもので、新規な白
血球分離材を提供することを目的とする。The present invention has been devised in view of the above points, and an object thereof is to provide a novel leukocyte separating material.
本発明はまた、白血球に対して高く安定した捕捉能を有
し、血液中より効率良く白血球を分離し得る白血球分離
材を提供することを目的とする。Another object of the present invention is to provide a leukocyte separating material which has a high and stable capturing ability for leukocytes and which can efficiently separate leukocytes from blood.
本発明はさらに、操作時における捕捉された白血球によ
る目詰まりやチャネリングの発生や繊維その他の異物の
流出の虞れがなく、完全に白血球除去操作を行い得る白
血球分離材を提供することを目的とする。Another object of the present invention is to provide a leukocyte separation material capable of performing a complete leukocyte removal operation without the risk of clogging or channeling due to captured leukocytes during operation or outflow of foreign substances such as fibers. To do.
（２）発明の構成 ＜問題点を解決するための手段＞ 上記本発明の諸目的は、 バブルポイントが0.08〜0.30Kg/cm2、厚さが0.30mm以上
である多孔質体からなる白血球分離材によって達成され
る。(2) Structure of the Invention <Means for Solving Problems> The above-mentioned various objects of the present invention are white blood cell separation composed of a porous body having a bubble point of 0.08 to 0.30 Kg / cm 2 and a thickness of 0.30 mm or more. Achieved by wood.
本発明はまた、バブルポイントは0.13〜0.25Kg/cm2であ
る多孔質体からなる白血球分離材を好ましいものとして
示すものである。本発明はさらに、多孔質体の厚さが0.
50mm以上である白血球分離材を好ましいものとして示す
ものである。The present invention also shows as a preferable leukocyte separating material comprising a porous body having a bubble point of 0.13 to 0.25 Kg / cm 2 . The present invention further has a thickness of the porous body of 0.
A leukocyte separating material having a diameter of 50 mm or more is shown as a preferable one.
＜作用＞ 本発明の白血球分離材は、白血球の分離を濾過法で行う
白血球に使用され、白血球と赤血球が混在する液体の中
から、白血球を選択分離する際に使用されるものであ
り、バブルポイントが0.08〜0.30Kg/cm2、厚さが0.30mm
以上である多孔質体からなることを特徴とするものであ
り、白血球分離能について、バブルポイントの大きさ
と、分離材の厚さの両方から規定されている。<Operation> The leukocyte separating material of the present invention is used for leukocytes in which leukocytes are separated by a filtration method, and is used for selectively separating leukocytes from a liquid in which leukocytes and erythrocytes are mixed. Point is 0.08 to 0.30 Kg / cm 2 , thickness is 0.30 mm
It is characterized by comprising the above porous body, and the leukocyte separation ability is defined by both the size of the bubble point and the thickness of the separating material.
バブルポイントとは、フィルターの分野で広く用いられ
ている言葉であり、完全に濡らされたフィルターの孔を
通って空気が押出されるときの圧力である。フィルター
は、微細で均一な毛細血管のようなものと考えることが
でき、一つの側から他の側へ貫通する通路を有してい
る。そこで、バブルポイントテストは、液体が表面張力
によりこれら毛細血管中に保持され、またこの毛細管か
ら液体を押出すのに必要な最低圧を知ることにより管径
を測定できるという事実に基いている。そうして、バブ
ルポイントテストは、直前にフィルターの片側に水を張
り完全に湿らせ、他の側の空気圧を次第に上げていき、
フィルターを通過する空気の着実で連続した微細な気泡
の流れが見られるときの圧力がバブルポイントである。Bubble point is a term widely used in the field of filters and is the pressure at which air is extruded through the pores of a fully wetted filter. Filters can be thought of as microscopic, uniform capillaries, with passages extending from one side to the other. The bubble point test is then based on the fact that liquid is retained in these capillaries by surface tension and that the tube diameter can be measured by knowing the minimum pressure required to push the liquid out of the capillaries. Then, in the bubble point test, immediately before filling the filter with water on one side to completely moisten it, gradually increase the air pressure on the other side,
The bubble point is the pressure at which a steady and continuous flow of fine air bubbles through the filter is seen.
実際、バブルポイントの測定は、例えば第３図のような
実験装置により行う。説明すると、空気送り込み管Ｋの
上端大径部の内周段部に、フィルターＦを水平に落し、
押えリングＲを螺合し、空気送り込み管Ｋの枝管にマノ
メーターＭを取付けた構造であり、フィルターＦの上に
水を張り、一旦、陰圧をかけてフィルターＦ中の空気を
水と置換し、空気送り込み管Ｋの下端より空気を、圧力
を徐々に上げながら送り込み、フィルターＦの上面より
連続した微細な気泡が出始めたとき、マノメータＭて読
取れる圧力がバブルポイントである。Actually, the bubble point is measured by an experimental device as shown in FIG. 3, for example. Explaining it, the filter F is dropped horizontally on the inner peripheral step of the large diameter portion of the upper end of the air feed pipe K,
It has a structure in which a holding ring R is screwed, and a manometer M is attached to a branch pipe of an air feeding pipe K. Water is put on the filter F and negative pressure is once applied to replace the air in the filter F with water. Then, when the air is fed from the lower end of the air feed pipe K while gradually increasing the pressure and continuous fine bubbles start to emerge from the upper surface of the filter F, the pressure read by the manometer M is the bubble point.
かかるバブルポイントは、多孔質体の気孔径のみならず
多孔質体の材質そのものの電荷、親水性の違い等の表面
状態によっても値が左右される。The value of the bubble point depends not only on the pore diameter of the porous body but also on the surface condition such as the electric charge and hydrophilicity difference of the material of the porous body itself.
本発明においては、バブルポイントの大きさの範囲の限
定と、分離材の最小厚さの限定との両面から、優れた白
血球分離能を有する白血球分離材を規定している。In the present invention, a leukocyte separation material having an excellent leukocyte separation ability is defined from both aspects of the range of bubble point size and the minimum separation material thickness.
これは、白血球の分離を濾過法に行うには、血球の大き
さ、変形能、異物に対する粘着性もしくは付着性の違い
から、単に白血球分離材（濾過素材）の気孔の大きさか
ら白血球分離能を規定するのは不適当で、バブルポイン
トの大きさからも規定するのが妥当であること、及び、
血液中に浮遊する血球の数は多く、効率良く持続的に白
血球を捕捉するためには、単に気孔径のみで白血球分離
能を規定する表面濾過は好ましくなく、濾過層の厚さを
前記バブルポイントの大きさと関連させて規定するのが
妥当だからである。This is because in order to separate white blood cells by the filtration method, the white blood cell size, the deformability, the difference in the adhesiveness or adhesiveness to foreign substances, and simply the white blood cell separation capacity (filtration material) can be used to separate the white blood cells. It is inappropriate to prescribe, and it is reasonable to prescribe from the size of the bubble point, and
The number of blood cells floating in the blood is large, and in order to efficiently and continuously capture leukocytes, surface filtration that regulates leukocyte separation ability only by the pore size is not preferable, and the thickness of the filtration layer is set to the bubble point. This is because it is appropriate to specify it in relation to the size of.
しかして、本発明は、バブルポイントが0.08〜0.30Kg/c
m2で、厚さが0.30mm以上の多孔質体を白血球分離材とし
て用いることで、白血球を効率良く除去するためのトラ
ップ空間が作り出されることを実験によって確認した。The bubble point of the present invention is 0.08 to 0.30 Kg / c.
It was confirmed by experiments that a trap space for efficiently removing leukocytes was created by using a porous body with m 2 and a thickness of 0.30 mm or more as a leukocyte separating material.
バブルポイントが0.13〜0.25Kg/cm2の範囲であると、白
血球分離効率が特に良好である。When the bubble point is in the range of 0.13 to 0.25 Kg / cm 2 , leukocyte separation efficiency is particularly good.
他方、バブルポイントが0.08Kg/cm2未満では白血球分離
効率が悪いことが確認された。また、バブルポイントが
0.30Kg/cm2よりも大きいと血液が殆ど流れなく、無理に
加圧等をして流すと、分離操作時に血球に損傷を与える
可能性がある。On the other hand, it was confirmed that leukocyte separation efficiency was poor when the bubble point was less than 0.08 Kg / cm 2 . Also, the bubble point
If it is greater than 0.30 Kg / cm 2 , blood hardly flows, and if pressure is applied forcibly, the blood cells may be damaged during the separation operation.
さらに、バブルポイントが0.08〜0.30Kg/cm2の範囲であ
っても、厚さが0.30mm未満のときは、分離効率が悪い。
これは、分離材と白血球との接触頻度が少くなるためと
考えられる。さらにまた、厚さが0.30mm未満のときは、
流れる血液の圧力で変形することがあり、分離膜として
の強度が弱いので、厚さを0.30mm未満とすることは好ま
しくない。0.50mm以上の厚さであれば、充分な分離効率
及び強度が確保される。Furthermore, even if the bubble point is in the range of 0.08 to 0.30 Kg / cm 2 , the separation efficiency is poor when the thickness is less than 0.30 mm.
It is considered that this is because the frequency of contact between the separation material and the white blood cells decreases. Furthermore, when the thickness is less than 0.30 mm,
It may be deformed by the pressure of flowing blood, and the strength as a separation membrane is weak, so it is not preferable to set the thickness to less than 0.30 mm. A thickness of 0.50 mm or more ensures sufficient separation efficiency and strength.
本発明の白血球分離材は、合成ゴム、熱可塑性樹脂、熱
硬化性樹脂、多孔金属より製作することができる。中で
も特に、ポリビニルアルコール系の多孔質体（鐘紡
（株）製、商品名ベルイーターＡ−3160）や、ポリウレ
タン系発泡体の多孔質体（東洋ポリマー（株）製、商品
名ルビセル）を採用して、バブルポイントが0.08〜0.30
Kg/cm2の範囲となるようにすることが好ましい。The leukocyte separating material of the present invention can be made of synthetic rubber, thermoplastic resin, thermosetting resin, or porous metal. Above all, a polyvinyl alcohol-based porous material (Kanebo Co., Ltd., trade name Bell Eater A-3160) and a polyurethane foam porous body (Toyo Polymer Co., Ltd., trade name Rubycell) are used. The bubble point is 0.08 to 0.30
It is preferable to set it in the range of Kg / cm 2 .
しかし、白血球分離材の材質が何であるかについては、
それが血液に与える影響が無いものであって、厚さが0.
30mm以上あり、バブルポイントが0.08〜0.30Kg/cm2であ
る白血球をトラップできる空間を作り出せば、問われる
ものではない。However, regarding the material of the leukocyte separation material,
It has no effect on blood and has a thickness of 0.
It does not matter if a space with a bubble point of 30 mm or more and a bubble point of 0.08 to 0.30 Kg / cm 2 can be trapped.
なお、多孔質体の気孔率は50〜90％であることが好まし
い。気孔率が50％未満であると血液処理速度が遅くな
り、また気孔率が90％を越えると強度が弱くなるからで
ある。The porosity of the porous body is preferably 50 to 90%. This is because when the porosity is less than 50%, the blood processing rate becomes slow, and when the porosity exceeds 90%, the strength becomes weak.
第１図は、本発明の白血球分離材を用いて白血球を分離
するための、一実施態様に係る白血球分離器の断面図で
ある。FIG. 1 is a sectional view of a leukocyte separator according to one embodiment for separating leukocytes using the leukocyte separating material of the present invention.
白血球分離器Ａは、上端に血液流入口1a、下端に流出口
1bを有し、中程より上下に分割されるハウジング１内
に、本発明の白血球分離材２が支持材3a、3bによりサン
ドイッチされてハウジング１内に水平に保持され、ハウ
ジング内部空間が上下に二分されてなるものである。The white blood cell separator A has a blood inlet 1a at the upper end and an outlet at the lower end.
The white blood cell separation material 2 of the present invention is sandwiched by the support materials 3a and 3b and horizontally held in the housing 1 which has 1b and is divided into upper and lower parts from the middle. It is divided into two parts.
第２図は、第１図の分離器を組入れた一実施態様に係る
白血球分離回路である。FIG. 2 is a white blood cell separation circuit according to an embodiment incorporating the separator of FIG.
この白血球分離回路は、処理しようとする血液を入れた
血液バッグ４及び生理食塩水を入れた生食バッグ５を白
血球分離用フィルター１より上位置に置いて、これらバ
ッグ４、５の液流出口と、白血球分離器Ａの血液流入口
1aとの間が、それぞれクレンメ6a、6bを具備してなる二
股状の導液チューブ７により連通されており、 他方、白血球分離器Ａの下方には、処理された血液を回
収するための血液回収用バッグ８と、生食回収用バッグ
９が位置され、これらバッグ８、９の液流入口と、白血
球分離器Ａの血液流出口1bとの間が、それぞれクレンメ
10a、10bを具備してなる二股状の導液チューブ11により
連通されている。In this leukocyte separation circuit, a blood bag 4 containing blood to be processed and a saline bag 5 containing physiological saline are placed above the leukocyte separation filter 1 and the liquid outlets of these bags 4 and 5 are placed. , Blood inlet of white blood cell separator A
1a is communicated with a bifurcated liquid-conducting tube 7 having clamps 6a and 6b, respectively. On the other hand, below the white blood cell separator A, blood for collecting treated blood is collected. A bag 8 for collecting and a bag 9 for collecting raw food are positioned, and the space between the liquid inlets of these bags 8 and 9 and the blood outlet 1b of the leukocyte separator A is respectively clean.
They are connected by a bifurcated liquid guide tube 11 including 10a and 10b.
白血球分離操作は、先ずクレンメ6b、10bを開き、クレ
ンメ6a、10aを閉じた状態とし、生食バッグ５から生食
液を白血球分離器Ａに流して、白血球分離器Ａ内をプラ
イミングし、この際、白血球分離器Ａ内を流下する生食
液は生食回収バッグ９に回収する。プライミングが行わ
れた後は、クレンメ6b、10bを閉じ、クレンメ6a,10aを
開いて血液バッグ４から血液を白血球分離器Ａに流す。
白血球分離器Ａ内において、血液は本発明の白血球分離
材２を通過する際に白血球成分が捕捉され分離される。In the white blood cell separation operation, first, the clamps 6b and 10b are opened and the clamps 6a and 10a are closed, and the saline is flowed from the saline bag 5 to the leukocyte separator A to prime the inside of the leukocyte separator A. The saline solution flowing down through the white blood cell separator A is collected in the saline collection bag 9. After the priming is performed, the clamps 6b and 10b are closed, the clamps 6a and 10a are opened, and the blood is flowed from the blood bag 4 to the leukocyte separator A.
In the white blood cell separator A, the white blood cell components are captured and separated when the blood passes through the white blood cell separation material 2 of the present invention.
そして、白血球成分を除去された血液は血液回収用バッ
グ８に回収される。Then, the blood from which the white blood cell component has been removed is collected in the blood collecting bag 8.
血液バッグ４より血液を流し終えたならクレンメ6aを閉
じるとともに、白血球分離器Ａ内に残った白血球成分を
除去された血液を回収するためにクレンメ6bを再び開
き、白血球分離器Ａ内に再び生食液を流して白血球分離
器Ａ内に残存する白血球成分を除去された血液を押出し
て血液回収用バッグ８に回収し、該回収をほぼ終えた時
点でクレンメ10aを閉じ、クレンメ10bを開いて血液回収
に用いた生食液を生食液回収用バッグ９内に回収する。
以上の操作により、白血球成分は、白血球分離器Ａの内
部に、正確には、本発明の白血球分離材２に白血球成分
が捕捉分離される。When the blood is completely drained from the blood bag 4, the clamp 6a is closed, the clamp 6b is opened again to collect the blood from which the leukocyte component remaining in the leukocyte separator A is recovered, and the leukocyte separator A is fed again with saline. The liquid is flowed to extrude the blood from which the white blood cell component remaining in the white blood cell separator A is extruded and collected in the blood collecting bag 8, and when the collection is almost completed, the clamp 10a is closed and the clamp 10b is opened to open the blood. The saline solution used for collection is collected in the saline bag 9 for collecting saline.
By the above operation, the white blood cell component is captured and separated inside the white blood cell separator A, more precisely, by the white blood cell separating material 2 of the present invention.
本発明者は、上記のように規定される本発明の白血球分
離材について、バブルポイント及び厚さが異なる種々の
ものを作成し、その各々について、該白血球分離材を第
１図の白血球分離器に取付け、第２図に示す白血球分離
回路により一定量の血液より白血球分離を行い、分離前
後の血球数の算定をELT-8（オーソダイアグノスティッ
ク社）にて行い、得られた白血球除去効率［WBC REM
（％）］を表−１及び表−２に示した。The present inventor created various leukocyte separating materials of the present invention defined as described above having different bubble points and thicknesses, and the leukocyte separating material of each of them was used as the leukocyte separator of FIG. The white blood cell is separated from a certain amount of blood by the white blood cell separation circuit shown in Fig. 2 and the blood cell count before and after the separation is calculated by ELT-8 (Ortho Diagnostic Co.). [WBC REM
(%)] Is shown in Table-1 and Table-2.
表−１の中の本発明品及び比較例（白血球分離効果が小
さく本発明品でないもの）は、いずれもポリビニルアル
コール系の多孔質体（鐘紡（株）製、商品名ベルイータ
ー Ａ−3160）からなる白血球分離材であり、表−２の
中の本発明品及び比較例は、いずれもポリウレタン系発
泡体の多孔質体からなる白血球分離材である。なお、膜
厚はいずれも乾燥状態における値を示す。The products of the present invention and comparative examples (those having a small leukocyte separation effect and not the products of the present invention) in Table 1 are all polyvinyl alcohol-based porous bodies (Bell Eater A-3160, manufactured by Kanebo Co., Ltd.). The white blood cell separating material according to the present invention and the comparative example in Table 2 are both white blood cell separating materials including a porous body of a polyurethane foam. In addition, all film thicknesses indicate values in a dry state.
（３）発明の効果 以上述べたように本発明の白血球分離材は、血液から簡
単な操作で白血球を効率良く捕捉除去することができ
る。そして、本発明の白血球分離材は、多孔質体である
ために繊維等の流出が少ない。さらに、本発明の白血球
分離材を用いて白血球分離器を製作する時は、本発明の
白血球分離材の容器への封入が非常に簡単になる。さら
に、本発明の白血球分離材は、多孔質体であるために捕
捉する白血球による目詰まりやチャネリングの発生や繊
維その他の異物の流出の虞れが少なく、白血球に対して
充分に安定した捕捉能を有する。 (3) Effects of the Invention As described above, the leukocyte separating material of the present invention can efficiently capture and remove leukocytes from blood by a simple operation. Since the leukocyte separating material of the present invention is a porous body, the outflow of fibers and the like is small. Furthermore, when a leukocyte separator is manufactured using the leukocyte separating material of the present invention, encapsulation of the leukocyte separating material of the present invention in a container becomes very easy. Further, since the leukocyte separating material of the present invention is a porous body, there is little risk of clogging and channeling due to leukocytes to be captured and outflow of fibers and other foreign substances, and a sufficiently stable leukocyte capturing ability. Have.
第１図は、本発明の白血球分離材を用いて白血球を分離
するための、一実施態様に係る白血球分離器の断面図で
ある。 第２図は、第１図の分離器を組入れた一実施態様に係る
白血球分離回路である。 第３図は、バブルポイントを測定するための装置の断面
図である。 Ｋ……空気送り込み管、Ｆ……フィルター、 Ｒ……押えリング、Ｍ……マノメーター、 Ａ……白血球分離器、１……ハウジング、 1a……血液流入口、1b……血液流出口、 ２……白血球分離材、3a、3b……支持材、 ４……血液バッグ、５……生食バッグ、 6a、6b……クレンメ、７……導液チューブ、 ８……生食回収用バッグ、９……血液回収用バッグ、 10a、10b……クレンメ、11……導液チューブ。FIG. 1 is a sectional view of a leukocyte separator according to one embodiment for separating leukocytes using the leukocyte separating material of the present invention. FIG. 2 is a white blood cell separation circuit according to an embodiment incorporating the separator of FIG. FIG. 3 is a sectional view of an apparatus for measuring the bubble point. K ... Air inlet pipe, F ... Filter, R ... Holding ring, M ... Manometer, A ... White blood cell separator, 1 ... Housing, 1a ... Blood inlet, 1b ... Blood outlet, 2 ...... Leukocyte separation material, 3a, 3b …… Support material, 4 …… Blood bag, 5 …… Salient food bag, 6a, 6b …… Clemme, 7 …… Conducting tube, 8 …… Salient food collection bag, 9 ・ ・ ・… Blood collection bag, 10a, 10b …… Clemme, 11 …… Conducting tube.
Claims (3)
が0.30mm以上である多孔質体からなる白血球分離材。1. A leukocyte separating material comprising a porous body having a bubble point of 0.08 to 0.30 Kg / cm 2 and a thickness of 0.30 mm or more.
請求項１記載の白血球分離材。2. The leukocyte separating material according to claim 1, wherein the bubble point is 0.13 to 0.25 Kg / cm 2 .
分離材。3. The leukocyte separating material according to claim 1, which has a thickness of 0.50 mm or more.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63050173A JPH0651631B2 (en) | 1988-03-03 | 1988-03-03 | Leukocyte separation material |
| CA000592188A CA1329559C (en) | 1988-03-03 | 1989-02-27 | Leukocyte separator and method of making the same |
| AU30870/89A AU606787B2 (en) | 1988-03-03 | 1989-03-01 | Leukocyte separator and method of making the same |
| DE89103675T DE68906807T2 (en) | 1988-03-03 | 1989-03-02 | Separator for the separation of leukocytes and process for its production. |
| EP89103675A EP0331174B1 (en) | 1988-03-03 | 1989-03-02 | Leukocyte separator and method of making the same |
| KR1019890002659A KR910002207B1 (en) | 1988-03-03 | 1989-03-03 | Liquip filtration device and method of making the same |
| US07/647,198 US5164087A (en) | 1988-03-03 | 1991-01-28 | Leukocyte separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63050173A JPH0651631B2 (en) | 1988-03-03 | 1988-03-03 | Leukocyte separation material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01224324A JPH01224324A (en) | 1989-09-07 |
| JPH0651631B2 true JPH0651631B2 (en) | 1994-07-06 |
Family
ID=12851811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63050173A Expired - Lifetime JPH0651631B2 (en) | 1988-03-03 | 1988-03-03 | Leukocyte separation material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0651631B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5498336A (en) * | 1991-02-22 | 1996-03-12 | Terumo Kabushiki Kaisha | Leukocyte-removing filter and leukocyte-removing apparatus furnished therewith |
| ATE197547T1 (en) * | 1991-08-22 | 2000-12-15 | Asahi Medical Co | FILTER MEDIUM FOR SELECTIVE REMOVAL OF LEUKOCYTES AND CORRESPONDING DEVICE |
| AU2003284534A1 (en) | 2002-12-02 | 2004-06-23 | Asahi Medical Co., Ltd. | Method of removing leukocytes, leukocyte-removing filter and utilization thereof |
| EP1754496B1 (en) | 2004-06-09 | 2016-01-20 | Asahi Kasei Medical Co., Ltd. | Method for removing leukocyte and filter for use therein |
| IT1401316B1 (en) * | 2010-08-06 | 2013-07-18 | Gvs Spa | FILTER PERFECTED FOR THE REMOVAL OF SUBSTANCES FROM THE BLOOD OR FROM EMODERIVED AND METHOD FOR ITS ACHIEVEMENT |
-
1988
- 1988-03-03 JP JP63050173A patent/JPH0651631B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01224324A (en) | 1989-09-07 |
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