JPH0642480Y2 - Cell culture plate - Google Patents
Cell culture plateInfo
- Publication number
- JPH0642480Y2 JPH0642480Y2 JP1988129261U JP12926188U JPH0642480Y2 JP H0642480 Y2 JPH0642480 Y2 JP H0642480Y2 JP 1988129261 U JP1988129261 U JP 1988129261U JP 12926188 U JP12926188 U JP 12926188U JP H0642480 Y2 JPH0642480 Y2 JP H0642480Y2
- Authority
- JP
- Japan
- Prior art keywords
- hole
- shape
- plate
- cell culture
- culture plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】 ［産業上の利用分野］ 本考案は微量の細胞であっても、効果的に培養ができる
プレートに関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to a plate capable of effectively culturing even a small amount of cells.
［従来の技術］ 細胞を培養するため、規則的に並べて使用する合成樹脂
製基板に孔を規則的に並べて設けたものを「プレート」
と通称していて、第３図の斜視図に示す構成であった。
第３図において、１は合成樹脂製基板、２は円筒形状の
中空の孔を示す。孔２は基板１上に縦横方向に規則的に
並べて設けられ、12×８＝96孔となっているプレートが
常用されている。その１個の孔は第４図の縦断面図に示
すように直径6.5mm、深さ約10mmで、隣接孔との間隔は
約1mmである。リンパ球・血液など細胞群を培養液0.2ml
と共に、所定位置の孔に上方から入れて、所定の温度・
湿度の条件下で所定時間培養する。培養した結果につい
て検査するときはプレートを水平位置としたまま、光学
顕微鏡の下に持って行き、孔の上方に対物レンズを動か
して検査することができる。[Prior Art] A "plate" is a synthetic resin substrate that is regularly arranged and used for culturing cells, in which holes are regularly arranged.
It has been commonly referred to as "," and has the configuration shown in the perspective view of FIG.
In FIG. 3, 1 is a synthetic resin substrate and 2 is a hollow cylindrical hole. The holes 2 are regularly arranged in the vertical and horizontal directions on the substrate 1, and a plate having 12 × 8 = 96 holes is commonly used. The one hole has a diameter of 6.5 mm and a depth of about 10 mm as shown in the longitudinal sectional view of FIG. 4, and the distance between adjacent holes is about 1 mm. 0.2 ml of culture medium containing cell groups such as lymphocytes and blood
At the same time, insert it into the hole at the specified position from above, and
Incubate for a predetermined time under humidity conditions. When inspecting the culture result, the plate can be brought under the optical microscope while the plate is in the horizontal position, and the objective lens can be moved above the hole to perform the inspection.
なお第３図・第４図に示す孔よりも、極めて小型の形状
で孔の縦断面形状を第５図に示すような、通称テラサキ
プレートと云うプレートも公知である。底部の直径は約
1mm、孔の上端部の直径は約4mmで、全体的に漏斗の形状
をしている。斜面は急激に上方に広がる形状である。な
お孔の間隔は約2mmである。Note that a plate commonly called a Terasaki plate, which has an extremely smaller shape than the holes shown in FIGS. 3 and 4 and whose longitudinal sectional shape is shown in FIG. 5, is also known. Bottom diameter is approx.
It is 1 mm in diameter and the diameter of the upper end of the hole is about 4 mm, and it has the shape of a funnel as a whole. The slope has a shape that spreads rapidly upward. The distance between the holes is about 2 mm.
［考案が解決しようとする課題］ 第３図・第４図に示すプレートでは、培養液・細胞群の
必要量が多く、培養後の検査に際し多過ぎることもあっ
た。テラサキプレートを使用するときは、その形状が小
さいため細胞群の必要量は少なくて済むが、孔の形状が
上方に拡がり過ぎているため、同一プレートの隣接する
孔に異なる細胞群を培養するときなど、隣接孔との中間
の合成樹脂基板上に細胞群を滴下させて、散って行くた
め隣接孔の細胞群を汚染することが起こり易い。またテ
ラサキプレートではその形状のため培養液が空気中に蒸
発し易かった。[Problems to be Solved by the Invention] The plates shown in FIGS. 3 and 4 required a large amount of the culture solution / cell group, and there were cases where the plates after the culture were too large. When using a Terasaki plate, the required size of the cell group is small because of its small shape, but when the different shape of the cell is cultivated in the adjacent holes of the same plate, the shape of the holes is too wide. For example, since the cell group is dropped on the synthetic resin substrate intermediate between the adjacent holes and scattered, it is easy to contaminate the cell group in the adjacent hole. In addition, because of the shape of the Terasaki plate, the culture solution was easily evaporated in the air.
本考案の目的は前述の欠点を改善し、少量の細胞群を使
用するのみで培養のできる形状の孔を設けた培養用プレ
ートを提供することにある。An object of the present invention is to improve the above-mentioned drawbacks and to provide a culture plate provided with a hole having a shape capable of culturing only by using a small amount of cells.
［課題を解決するための手段］ 前述の課題を解決するため本考案が採用した手段は下記
のとおりである。即ち、 ほヾ透明な合成樹脂製基板にほヾ円筒形状の孔を縦横方
向に規則的に並べて設けた細胞培養用プレートにおい
て、円筒形の孔の底部は円形であって、且つ上端部より
狭い形状とし、孔は上方へ向かって漏斗状に拡大し、且
つその内壁が底部となす角度が約60度となっていること
である。[Means for Solving the Problems] Means adopted by the present invention for solving the above problems are as follows. That is, in a cell culture plate in which a substantially transparent substrate made of a synthetic resin and holes of a substantially cylindrical shape are regularly arranged in the vertical and horizontal directions, the bottom of the cylindrical hole is circular and narrower than the upper end. The shape is such that the hole expands upward in a funnel shape, and the angle between the inner wall and the bottom is about 60 degrees.
［作用］ 本考案による細胞培養用プレートの孔の形状が、孔の途
中から漏斗状となっているため、培養のため使用する細
胞群の量が従来より極めて少量で済むようになった。[Operation] Since the shape of the hole of the cell culture plate according to the present invention is funnel-shaped from the middle of the hole, the amount of cells used for culture can be much smaller than before.
［実施例］ 第１図は本考案の一実施例としてプレートの一部につい
て縦断面形状を示す概略図、第２図は１個の孔について
の断面形状を示す図である。第１図において、１はほヾ
透明な合成樹脂製の基板、3-1,3-2……は本考案による
孔を示す。孔3-1などの底部４は第２図に示すように、
例えば直径2mmでその両端から漏斗状に拡がる斜面５は
例えば底部４とのなす角度が約60度である。漏斗状部分
の深さは約3.5mmとなる。円筒状となっている孔の部分
６はその深さが約8mmで、この孔６は上方端部へ行く
程、極く僅か拡がるように微量の傾斜を持つことが、樹
脂の金型を製造する場合を含み金型から取り出すときな
どに都合が良い。[Embodiment] FIG. 1 is a schematic view showing a vertical cross-sectional shape of a part of a plate as one embodiment of the present invention, and FIG. 2 is a view showing a cross-sectional shape of one hole. In FIG. 1, reference numeral 1 is a transparent substrate made of synthetic resin, and 3-1, 3-2 ... Show holes according to the present invention. As shown in FIG. 2, the bottom 4 such as the hole 3-1 is
For example, an inclined surface 5 having a diameter of 2 mm and extending in a funnel shape from both ends thereof forms an angle of about 60 degrees with the bottom portion 4, for example. The depth of the funnel-shaped part is about 3.5 mm. The cylindrical hole portion 6 has a depth of about 8 mm, and the hole 6 has a slight inclination so that the hole 6 expands slightly toward the upper end. It is convenient when taking out from the mold including the case.
［考案の効果］ このようにして本考案によると、細胞培養用プレートの
孔底部の面積が孔の上端部面積に比べて狭く、孔の底部
から漏斗状に拡大しているから、培養のため孔に入れら
れた培養液の容量は従来より少なくても、所定の培養の
処理を従来と同様に行うことができる。例えば前述の実
施例によると量は1/10の約0.02mlで済む。それは従来の
第３図に示すプレートは培養液が少量のとき、孔底部全
体に拡がらず、空気に接触する面積が大きく、蒸発量が
多いからである。[Advantage of the Invention] According to the present invention, the area of the bottom of the hole of the cell culture plate is narrower than the area of the upper end of the hole, and the funnel shape expands from the bottom of the hole. Even if the volume of the culture solution put in the holes is smaller than the conventional one, the predetermined culture treatment can be performed in the same manner as the conventional one. For example, according to the above-mentioned embodiment, the amount is about 0.02 ml, which is 1/10. This is because the conventional plate shown in FIG. 3 does not spread over the entire bottom of the hole when the amount of culture solution is small, the area in contact with air is large, and the amount of evaporation is large.
また、少量の培養液を処理するから、処理途中におい
て、光学顕微鏡を使用して観察しながら、培養液を部分
的に取出したり、必要に応じ追加することが容易であ
り、そのため細胞のクローニングがしやすい。第４図に
示すテラサキプレートでは培養液を追加することは非常
に困難であった。In addition, since a small amount of the culture solution is processed, it is easy to partially remove the culture solution or add it as necessary while observing it using an optical microscope during the process, and thus cell cloning is possible. It's easy to do. It was very difficult to add the culture solution to the Terasaki plate shown in FIG.
次に孔は円筒状部分があるため、細胞群を孔に入れると
き所定のもの以外を間隔部或いは隣接孔に誤って入れる
機会が少なくなり、細菌などによる汚染が防止できる。Next, since the hole has a cylindrical portion, when a cell group is put into the hole, it is less likely to mistakenly insert a cell other than a predetermined one into the gap or the adjacent hole, and contamination with bacteria or the like can be prevented.
第１図は本考案の実施例の構成を示す図、 第２図は第１図の部分構成を示す図、 第３図は従来のプレートと孔の形状を示す斜視図、 第４図は第３図に示す孔の断面図、 第５図はテラサキプレートの孔の断面図である。 １……合成樹脂基板 2,3-1,3-2……孔 ４……底部 ５……斜面 FIG. 1 is a diagram showing a configuration of an embodiment of the present invention, FIG. 2 is a diagram showing a partial configuration of FIG. 1, FIG. 3 is a perspective view showing a conventional plate and hole shape, and FIG. FIG. 3 is a sectional view of the hole shown in FIG. 3, and FIG. 5 is a sectional view of the hole of the Terasaki plate. 1 …… Synthetic resin substrate 2,3-1,3-2 …… Hole 4 …… Bottom 5 …… Slope
Claims (1)
の孔を縦横方向に規則的に並べて設けた細胞培養用プレ
ートにおいて、 前記孔の底部は円形であって、且つ上端部より狭い面積
とし、孔は底部から上方へ向かって漏斗状に拡大し、且
つその内壁が底部となす角度が約60度となっていて、漏
斗状に拡大する孔の横断面形状は上端部の横断面形状に
達する部分から上の部分が円筒形であること を特徴とする細胞培養用プレート。1. A cell culture plate comprising a substantially transparent substrate made of synthetic resin and having substantially cylindrical holes regularly arranged in the longitudinal and lateral directions, wherein the bottom of the hole is circular, and The area is narrow, the hole expands upward from the bottom in a funnel shape, and the inner wall makes an angle of about 60 degrees with the bottom. A cell culture plate characterized in that the portion from the portion reaching the surface shape to the upper portion is cylindrical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1988129261U JPH0642480Y2 (en) | 1988-10-02 | 1988-10-02 | Cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1988129261U JPH0642480Y2 (en) | 1988-10-02 | 1988-10-02 | Cell culture plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0249898U JPH0249898U (en) | 1990-04-06 |
| JPH0642480Y2 true JPH0642480Y2 (en) | 1994-11-09 |
Family
ID=31383376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1988129261U Expired - Lifetime JPH0642480Y2 (en) | 1988-10-02 | 1988-10-02 | Cell culture plate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0642480Y2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2716646B2 (en) * | 1993-05-21 | 1998-02-18 | 住友ベークライト株式会社 | Method for forming cell aggregates |
| JP4665589B2 (en) * | 2005-04-01 | 2011-04-06 | ニプロ株式会社 | Cell culture vessel |
| EP2213721B1 (en) | 2007-09-18 | 2016-03-02 | Reprocell, Inc. | Cell measuring vessel, extracellular potential measuring method, and chemical testing method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61247377A (en) * | 1985-04-26 | 1986-11-04 | Hitachi Ltd | Culture vessel for measuring microorganisms and detection of microorganism therewith |
-
1988
- 1988-10-02 JP JP1988129261U patent/JPH0642480Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0249898U (en) | 1990-04-06 |
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