JPH06304245A - Separation of blood - Google Patents
Separation of bloodInfo
- Publication number
- JPH06304245A JPH06304245A JP5120714A JP12071493A JPH06304245A JP H06304245 A JPH06304245 A JP H06304245A JP 5120714 A JP5120714 A JP 5120714A JP 12071493 A JP12071493 A JP 12071493A JP H06304245 A JPH06304245 A JP H06304245A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- bag
- layer
- component
- lower layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- External Artificial Organs (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、血液を各成分ごとに分
離するために用いられる血液バツグセツトに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood bag set used for separating blood into individual components.
【０００２】[0002]
【従来の技術】全血に含まれる多様な血液成分を取り出
して、製剤化する際には、血液バツグセツトが使用され
る。2. Description of the Related Art A blood bag set is used for taking out various blood components contained in whole blood and formulating them.
【０００３】親バツグに採取された全血は、通常、遠心
分離操作により、低密度成分を上層に高密度成分を下層
に分離する。その際、白血球を多分に含有するバフイー
コートと称する中間層もできる。それらを各成分ごとに
取り出す血液バツグセツトの一例としては、米国特許明
細書第５，１０２，４０７号に示すものがある。The whole blood collected in the parent bag is usually centrifuged to separate low-density components into upper layers and high-density components into lower layers. At that time, an intermediate layer called buffy coat containing a large amount of white blood cells can also be formed. An example of a blood bag set for extracting each of these components is shown in US Pat. No. 5,102,407.
【０００４】このものでは、上層の低密度の血液成分を
取り出すために、親バツグ内部の上端で開口する流出口
があり、下層の高密度の血液成分を取り出すために、同
親バツグ内部にチユーブ状で挿入され且つその下端で開
口する流出口が備えられている。その結果バフイーコー
トは親バツグに残されることになる。In this device, there is an outlet opening at the upper end inside the parent bag in order to take out the low-density blood component in the upper layer, and in order to take out the high-density blood component in the lower layer, the tube inside the parent bag. An outlet is provided which is inserted in the shape of and is open at its lower end. As a result, the buffy court will be left in the parent bag.
【０００５】[0005]
【発明が解決しようとする課題】上記の方法であると、
全血を親バツグ内に採取したとき、下層の高密度の血液
成分を取り出すための下端で開口するチユーブ内にも全
血が侵入し、それが遠心分離されれば、それから発生し
たバフイーコートがそのチユーブ内で存在したままとな
る。[Problems to be Solved by the Invention] According to the above method,
When whole blood is collected in the parent bag, whole blood also enters the tube that opens at the lower end to take out the high-density blood components of the lower layer, and if it is centrifuged, the buffy coat generated from it will It will still exist in the tube.
【０００６】その結果、下層の高密度の血液成分を取り
出しても白血球が混入するという問題があった。本発明
は下層の高密度の血液成分に白血球を混入させないよう
にした血液分離方法を提供するものである。As a result, there is a problem in that white blood cells are mixed even if the high-density blood component of the lower layer is taken out. The present invention provides a blood separation method in which white blood cells are prevented from being mixed with the high-density blood component of the lower layer.
【０００７】[0007]
【課題を解決するための手段】本発明は、（ａ）バツグ
の底に２つの流出口を有し、バツグの底から上方に向か
って長さ方向に融着部分がバツグの少なくとも半分以上
上まで延びて、その融着部分によって上記２つの流出口
を隔てた血液バツグに、全血を採取し、（ｂ）次に、遠
心分離操作により、上記バツグ内の全血を低密度成分を
上層に高密度成分を下層に分離し、（ｃ）上記バツグの
上から上層の低密度成分を上記シール部分を伝わらせな
がら、それに通じている流出口から取り出し、（ｄ）他
の流出口から下層の高密度成分を取り出すことを特徴と
する血液分離方法を要旨とする。According to the present invention, (a) two outlets are provided at the bottom of a bag, and a fusion-bonded portion is at least a half or more of the bag in the lengthwise direction upward from the bottom of the bag. Whole blood is collected in a blood bag which extends to the blood bag separating the two outlets by the fused portion, and (b) is then subjected to a centrifugation operation to lower the whole blood in the bag to a lower density component. The high-density component is separated into a lower layer, and (c) the low-density component of the upper layer is taken from the above-mentioned bag while being transmitted through the above-mentioned sealing portion, and is taken out from the outlet communicating therewith, and A gist of the present invention is a blood separation method characterized by extracting high-density components of.
【０００８】本発明は構造において以上のような特徴を
有する親バツグを用いた血液分離方法である。本発明に
おいて全血とは血液成分の分離操作を受ける前の人体か
ら採取された血液である。The present invention is a blood separation method using a parent bag having the above-mentioned characteristics in structure. In the present invention, whole blood is blood collected from a human body before being subjected to a blood component separation operation.
【０００９】本発明においてバツグの底から上方に向か
って長さ方向に融着部分がバツグの少なくとも半分以上
上まで延びて、その融着部分によって、親バツグ内に狭
小部ができるが、この大きさで血液包含時の狭小部の横
断面積を血液バツグセツトに用いられるチユーブ内部の
横断面積と同程度にすると、血液分離の操作上好まし
い。In the present invention, the fusion-bonding portion extends upward from the bottom of the bag in the lengthwise direction to at least half or more of the bag, and the fusion-bonding portion forms a narrow portion in the parent bag. When the blood is included, the cross-sectional area of the narrowed portion should be approximately the same as the cross-sectional area of the inside of the tube used for the blood bag set, which is preferable for the operation of blood separation.
【００１０】[0010]
【作用】全血をまず親バツグに採取して遠心分離する
と、親バツグ内部では密度の差によって、血漿の上部層
とバフイーコート、赤血球を含有する下部層がそれぞれ
分離形成される。When whole blood is first collected in the parent bag and centrifuged, the upper layer of plasma and the lower layer containing buffy coat and red blood cells are separated and formed inside the parent bag due to the difference in density.
【００１１】その後、親バツグを圧搾して親バツグ内部
の上部と下部にある各血液成分を上部層用流出口、下部
層用流出口からおのおの子バツグに移送する。その結果
親バツグにはバフイーコートが残留することになる。親
バツグ内の上下の層を同時に親バツグから排出できるの
も、本発明の特徴である。After that, the parent bag is squeezed to transfer the blood components in the upper and lower parts inside the parent bag from the upper layer outlet and the lower layer outlet to each child bag. As a result, the buffy coat remains on the parent bug. It is also a feature of the present invention that the upper and lower layers in the parent bag can be discharged simultaneously from the parent bag.
【００１２】上記の際に、下部層用流出口が親バツグの
下部に備えられ、遠心分離後バフイーコートと下部層の
境界線を保ちながら、下部層を流出するときはバフイー
コートを混入させるおそれはない。In the above case, the lower layer outlet is provided in the lower part of the parent bag, and there is no risk of mixing the buffy coat when the lower layer flows out while keeping the boundary line between the buffy coat and the lower layer after centrifugation. .
【００１３】また、狭小部の開放位置がバツグの少なく
とも半分以上上に位置しているので、全血を親バツグに
採取するとき狭小部に全血が入らないように注意するこ
とができる。その結果、バフイーコートが混入していな
い血漿を分離・採取することができる。Further, since the open position of the narrow portion is located at least above half of the bag, it is possible to take care so that the whole blood does not enter the narrow portion when the whole blood is collected in the parent bag. As a result, plasma free from buffy coat can be separated and collected.
【００１４】遠心分離で得られた血漿すなわち上部層を
流出口から採取するとき、本発明のような狭小部を設け
る代わりに、流出口をバツグの少なくとも半分以上上ま
で延ばしたチユーブにしておいても、上記作用・効果を
示すが、バツグ内にチユーブがあると、遠心分離操作に
おいて、チユーブがバツグ本体に突起状の傷をつける原
因となり、好ましくない。また上部加圧式或いは下部加
圧式自動分離装置によって分離すると、バツグ内のチユ
ーブが挟まれた状態となって分離速度が落ちることとな
り、更には分離不能となる。When collecting the plasma obtained by centrifugation, that is, the upper layer, from the outlet, instead of providing the narrow portion as in the present invention, the outlet is made a tube extending to at least half or more of the bag. Although the above-mentioned action and effect are exhibited, the presence of the tube in the bag is not preferable because the tube causes a scratch on the bag body during the centrifugal separation operation. Further, when the upper pressure type or the lower pressure type automatic separating device is used for separation, the tubes in the bag are sandwiched and the separation speed is lowered, and further the separation becomes impossible.
【００１５】[0015]
【実施例】以下、本発明の一実施例を図１の図面に基づ
き説明する。図１は血液バツグセツトの一部である本発
明による親バツグの実施例を示す。親バツグ１は全血が
採取されるもので、本体２により主構成され、この本体
２は、ポリ塩化ビニル等の合成樹脂製の２枚のシートか
ら構成されて、これらシートの周囲が熱融着か高周波溶
接によって接着されている。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawing of FIG. FIG. 1 shows an embodiment of a parent bag according to the invention which is part of a blood bag set. The parent bag 1 is for collecting whole blood, and is mainly composed of a main body 2. The main body 2 is composed of two sheets of synthetic resin such as polyvinyl chloride, and the periphery of these sheets is heat-melted. Adhered by wearing or high frequency welding.
【００１６】本体２の下端部には、採血チユーブによる
注入口３と、上部層用・下部層用流出口４、５が備えら
れている。また、親バツグ内部を融着により上下方向に
仕切ることによって、親バツグ縁部との間で、開放箇所
６を有する狭小部７が設けられている。The lower end of the main body 2 is provided with an inlet 3 for collecting blood and an outlet 4, 5 for upper and lower layers. Further, by narrowing the inside of the parent bag in the vertical direction by fusing, a narrow portion 7 having an open portion 6 is provided between the parent bag and the edge of the parent bag.
【００１７】上部層用・下部層用流出口４、５は、例え
ば、ポリ塩化ビニル等の合成樹脂製の軟質チユーブによ
り構成されており、本体８の２枚のシートを接着する際
に、両シート間に挟み込まれて固定される。上部層用・
下部層用流出口４、５の下部は、本体２の下方に突出す
ると共に、折り曲げることにより本体２内部と外部を連
通させる連通ピース（俗に折れ棒という。）が備えられ
ている。The upper layer / lower layer outlets 4 and 5 are made of, for example, a soft tube made of a synthetic resin such as polyvinyl chloride, and are used to bond the two sheets of the main body 8 together. It is fixed by being sandwiched between the sheets. For upper layer
The lower portions of the lower layer outlets 4 and 5 are provided with a communicating piece (commonly called a bent bar) that protrudes below the main body 2 and connects the inside and the outside of the main body 2 by bending.
【００１８】下部層用流出口５は本体２内部の下端部で
開口し、また、上部層用流出口４は狭小部７内部の親バ
ツグ下端部で開口している。なお本体２内部には、ＣＰ
Ｄ液、ＡＣＤ−Ａ液等の血液の保存液が封入されている
ことがある。The lower layer outlet 5 opens at the lower end inside the main body 2, and the upper layer outlet 4 opens at the lower end of the parent bag inside the narrow portion 7. In addition, inside the main body 2, CP
A blood preservation solution such as D solution or ACD-A solution may be enclosed.
【００１９】上記のように構成した実施例によれば、全
血を採血して遠心分離する際には、まず採血針と採血チ
ユーブにより注入口３を介して、血液提供者より全血を
親バツグ１の本体２内部に導入した後、採血チユーブを
流出口３から遮断する。According to the embodiment configured as described above, when whole blood is collected and centrifuged, the whole blood is first collected from the blood donor via the inlet 3 by the blood collecting needle and the blood collecting tube. After being introduced into the body 2 of the bag 1, the blood collection tube is blocked from the outlet 3.
【００２０】そして親バツグはもとより血液バツグセツ
トを遠心分離装置内に入れて本体２内部の血液を４４０
０×ｇ、６分で遠心分離すると、本体２内部の上部には
血漿の上部層が、中間部にはバフイーコートが、下部に
は赤血球を主として含有する下部層が、それぞれ分離形
成される。Then, not only the parent bag but also the blood bag set is put in the centrifuge and the blood in the main body 2 is 440
By centrifugation at 0 × g for 6 minutes, an upper layer of plasma is formed in the upper part inside the main body 2, a buffy coat is formed in the middle part, and a lower layer mainly containing red blood cells is formed in the lower part.
【００２１】そして上記のように全血を遠心分離した
後、連通ピースを折り曲げて、上部層用・下部層用流出
口４、５から上部層用・下部層用チユーブを介して、親
バツグ１の本体２内部を上部層用・下部層用子バツグに
連通させる。そして親バツグ１を、例えば、血液分離用
圧搾装置で圧縮して、本体２の内部の上部にある血液成
分すなわち血漿を、狭小部７を通し、上部層用チユーブ
を介して、上部層用子バツグに移送する。同時に本体２
の内部の下部にある血液成分を、下部層用チユーブを介
して、下部層用子バツグに移送する。After centrifuging the whole blood as described above, the communication piece is bent and the parent bag 1 is passed from the upper layer / lower layer outlets 4 and 5 through the upper layer / lower layer tubes. The inside of the main body 2 is communicated with the upper layer / lower layer child bag. Then, the parent bag 1 is compressed by, for example, a blood separation squeezing device, and the blood component in the upper portion of the main body 2, that is, plasma, is passed through the narrow portion 7, and the upper layer child is passed through the upper layer tube. Transfer to Bagu. Body 2 at the same time
The blood component in the lower part of the inside of the container is transferred to the lower layer child bag through the lower layer tube.
【００２２】[0022]
【発明の効果】以上詳述したように、本発明によれば、
バフイーコートが混入していない、品質の優れた血液製
剤が得られる。また本発明によれば、全血から血液成分
を取り出す作業において、血液バツグセツトを遠心分離
装置にかけた際に、親バツグに突起状の傷が形成されに
くく、親バツグがその傷で破損されるおそれが少ない。
更にまた、本発明の血液分離方法は省力化及び作業時間
の短縮の点で非常に有利である。As described in detail above, according to the present invention,
A high-quality blood product containing no buffy coat can be obtained. Further, according to the present invention, in the operation of extracting blood components from whole blood, when the blood bag set is subjected to a centrifuge, it is difficult to form a projecting scratch on the parent bag, and the parent bag may be damaged by the scratch. Less is.
Furthermore, the blood separation method of the present invention is very advantageous in terms of labor saving and working time reduction.
【図１】血液バツグセツトの一部である本発明による親
バツグの実施例を示す。1 shows an example of a parent bag according to the invention which is part of a blood bag set.
１ 親バツグ ２ 本体 ４ 上部層用流出口 ７ 狭小部 1 Parent bag 2 Main body 4 Upper layer outlet 7 Narrow part
Claims (1)
し、バツグの底から上方に向かって長さ方向に融着部分
がバツグの少なくとも半分以上上まで延びて、その融着
部分によって上記２つの流出口を隔てた血液バツグに、
全血を採取し、（ｂ）次に、遠心分離操作により、上記
バツグ内の全血を低密度成分を上層に高密度成分を下層
に分離し、（ｃ）上記バツグの上から上層の低密度成分
を上記シール部分を伝わらせながら、それに通じている
流出口から取り出し、（ｄ）他の流出口から下層の高密
度成分を取り出すことを特徴とする血液分離方法。1. (a) Two outlets are provided at the bottom of the bag, and the fused portion extends upward from the bottom of the bag in the lengthwise direction to at least half or more of the bag, and the fused portion. To the blood bag separating the above two outlets,
Whole blood was collected, and (b) the whole blood in the bag was separated into a low-density component and a high-density component in the lower layer by centrifugation, and (c) a lower layer from the upper layer to the upper layer. A blood separation method, wherein a density component is taken out from an outlet communicating with the sealed portion while being transmitted through the sealed portion, and (d) a high-density component of a lower layer is taken out from another outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12071493A JP3163608B2 (en) | 1993-04-23 | 1993-04-23 | Blood separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12071493A JP3163608B2 (en) | 1993-04-23 | 1993-04-23 | Blood separation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06304245A true JPH06304245A (en) | 1994-11-01 |
| JP3163608B2 JP3163608B2 (en) | 2001-05-08 |
Family
ID=14793187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12071493A Expired - Fee Related JP3163608B2 (en) | 1993-04-23 | 1993-04-23 | Blood separation method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3163608B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009089919A (en) * | 2007-10-10 | 2009-04-30 | Kawasumi Lab Inc | Blood collecting device |
| JP2009100868A (en) * | 2007-10-22 | 2009-05-14 | Kawasumi Lab Inc | Sampling bag and blood drawing implement |
| CN103191016A (en) * | 2012-01-09 | 2013-07-10 | 金卫医疗科技(上海)有限公司 | Separation soft bag used for increasing blood plasma extraction purity during continuous blood separation |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102193288B1 (en) * | 2019-01-07 | 2020-12-22 | 주식회사 윤진 | Multi-function fork with tongs function for spaghetti |
| KR102193287B1 (en) * | 2019-01-07 | 2020-12-22 | 주식회사 윤진 | Multi-function spoon with tongs function for noodle |
-
1993
- 1993-04-23 JP JP12071493A patent/JP3163608B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009089919A (en) * | 2007-10-10 | 2009-04-30 | Kawasumi Lab Inc | Blood collecting device |
| JP2009100868A (en) * | 2007-10-22 | 2009-05-14 | Kawasumi Lab Inc | Sampling bag and blood drawing implement |
| CN103191016A (en) * | 2012-01-09 | 2013-07-10 | 金卫医疗科技(上海)有限公司 | Separation soft bag used for increasing blood plasma extraction purity during continuous blood separation |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3163608B2 (en) | 2001-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |