JPH06287199A - Method for purifying beet saponin - Google Patents
Method for purifying beet saponinInfo
- Publication number
- JPH06287199A JPH06287199A JP5098424A JP9842493A JPH06287199A JP H06287199 A JPH06287199 A JP H06287199A JP 5098424 A JP5098424 A JP 5098424A JP 9842493 A JP9842493 A JP 9842493A JP H06287199 A JPH06287199 A JP H06287199A
- Authority
- JP
- Japan
- Prior art keywords
- saponin
- beet
- aqueous solution
- extract
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Steroid Compounds (AREA)
- Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Description
【発明の詳細な説明】Detailed Description of the Invention
【０００１】[0001]
【産業上の利用分野】この発明は、てん菜から抽出した
粗サポニン溶液を精製する方法に関するものである。TECHNICAL FIELD The present invention relates to a method for purifying a crude saponin solution extracted from sugar beet.
【０００２】[0002]
【従来の技術】てん菜は、アカザ科の植物で、現在では
一般にビートと呼称されており、この菜根より砂糖を製
造するために大量に栽培されている。BACKGROUND OF THE INVENTION Sugar beet is a plant of the family Chenopodiaceae, which is now generally called beet, and is cultivated in large quantities to produce sugar from this rape root.
【０００３】ビートは気泡性の強いサポニンを含有して
おり、砂糖の抽出段階で一部抽出液中に移行してくるた
め、製造工程の糖液は泡立ちが多くなり、精製の阻害要
因となり、生産能率の低下を来している。Beet contains saponin, which has a strong foaming property, and it partially migrates into the extract during the sugar extraction stage, so that the sugar liquid in the manufacturing process becomes more foamy and becomes an inhibitory factor for purification. The production efficiency is declining.
【０００４】ビートサポニンは、ビート根中に0.1%前後
含まれており、この一部が抽出されてジュース中に移行
するが、その抽出残渣、即ちビートパルプに乾物換算0.
3 〜0.9%のサポニンが残存する。なお、ビート中のサポ
ニンの測定数値を第一表に示す。Beet saponin is contained in the beet root in an amount of about 0.1%, and a part of this is extracted and transferred to juice.
3-0.9% saponin remains. Table 1 shows the measured values of saponin in beet.
【０００５】[0005]
【表１】 [Table 1]
【０００６】このビートサポニンは、オレアノール酸の
配糖体であることが確認されているが、有効利用につい
ての研究が十分に行われていない。It has been confirmed that beet saponin is a glycoside of oleanolic acid, but studies on its effective use have not been sufficiently conducted.
【０００７】これに対して、食品・化粧品等において使
用される界面活性剤等の添加物は合成品が大勢を占め、
天然の界面活性剤はキラヤサポニン等が市販されている
のみであるが、最近の一般消費者には天然物嗜好があ
り、このような趨勢から本願発明者らはビート中に含ま
れているサポニンを天然の界面活性剤として活用するた
め、安価で、且つ幅広い用途とするため、品質の良好な
サポニンの製造方法の検討を重ねた。On the other hand, synthetic products account for the majority of additives such as surfactants used in foods and cosmetics.
Quillaya saponin and the like are the only natural surfactants on the market, but recent general consumers have a preference for natural products, and due to this trend, the present inventors have found that saponin contained in beetroot. Since it is used as a natural surfactant, it is inexpensive and has a wide range of applications. Therefore, a method for producing saponin of good quality was studied.
【０００８】ところが、ビートサポニンの有効な利用方
法が今迄開発されていないため、ビートサポニンの製造
方法並びに精製方法の報告は殆どなく、本願発明者の一
人が特公平5-7399号及び特開平4-103598号に提案してい
る程度である。However, since an effective use method of beet saponin has not been developed so far, there is almost no report on a method for producing beet saponin and a method for purifying beet saponin, and one of the inventors of the present application is Japanese Patent Publication No. 7399/1993. It is about the level proposed in No. 4-103598.
【０００９】[0009]
【発明が解決しようとする問題点】しかし、上記特公平
5-7399号にはてん菜粉砕物をアルカリ水溶液で抽出処理
し、得られた抽出液を合成吸着樹脂に接触させてビート
サポニンを吸着させる方法が記載されているが、この方
法では、回収率については目的を達しているが、サポニ
ンの純度が約25% と低く、これから精製を進めても含ま
れている不純物の成分の関係から、純度の向上がかなり
難しく、コストが非常に高くなる等の欠点がある。[Problems to be Solved by the Invention]
No. 5-7399 describes a method of extracting beet crushed product with an alkaline aqueous solution, and adsorbing beet saponin by contacting the resulting extract with a synthetic adsorption resin. However, the purity of saponin is as low as about 25%, and it is difficult to improve the purity due to the relation of impurities contained in the product even if purification is proceeded from now on. There are drawbacks.
【００１０】また特開平4-103598号には上記特公平5-73
99号の方法の改良としててん菜粉砕物のアルカリ抽出液
を限外濾過により高分子成分を除去し、更に遠心分離す
る方法或はてん菜粉砕物のアルカリ抽出液を限外濾過に
より高分子成分を除去し、更に合成吸着樹脂に接触させ
る方法が記載されるが、この方法では純度が、62% と改
良されているが、製品がかなり着色しており、収率も若
干低下する。Further, Japanese Patent Application Laid-Open No. 4-103598 describes the above-mentioned Japanese Patent Publication No. 5-73.
As a modification of the method of No. 99, the polymer component is removed from the alkaline extract of the crushed sugar beet by ultrafiltration and further centrifuged, or the alkaline extract of the crushed sugar beet is removed by ultrafiltration to remove the polymer component. Furthermore, a method of contacting with a synthetic adsorption resin is described, but the purity is improved to 62% by this method, but the product is considerably colored and the yield is slightly reduced.
【００１１】本願発明者らは前述の問題点を解決するた
め、鋭意研究を重ね、従来、ビートサポニンは活性炭に
吸着され易く、大幅な回収率の低下を来すことから、精
製に活性炭を使用することができなかったが、有機溶媒
をサポニン水溶液に混合使用することにより、活性炭へ
のサポニン吸着を阻止し、室温において効率的に精製す
ることができることを見出した。In order to solve the above-mentioned problems, the inventors of the present invention have conducted extensive studies, and in the past, beet saponin was easily adsorbed on activated carbon, resulting in a large decrease in recovery rate. Therefore, activated carbon was used for purification. However, it has been found that, by mixing and using an organic solvent with an aqueous saponin solution, adsorption of saponin on activated carbon can be prevented, and purification can be efficiently performed at room temperature.
【００１２】[0012]
【問題点を解決するための手段】この発明は、上記知見
に基づいて完成したものであり、本願の第１発明はてん
菜の粉砕物、スライス物、又はてん菜をスライスし浸出
装置にて糖を抽出した残渣（以下、プレスドパルプとい
う）を水又はアルカリ水溶液でサポニンを抽出し、得ら
れた抽出液中のペクチン等の高分子成分を例えば限外濾
過処理により除去し、酸によりサポニンフロックを形成
し、このフロックをアルカリ水溶液にて溶解し、有機溶
媒及び活性炭を添加処理して精製するビートサポニンの
精製方法を提案するものである。The present invention has been completed based on the above findings, and the first invention of the present application is a crushed product of sugar beet, a sliced product, or a sugar beet sliced and sugar is extracted by a leaching device. Saponin is extracted from the extracted residue (hereinafter referred to as pressed pulp) with water or an alkaline aqueous solution, and high molecular components such as pectin in the obtained extract are removed by, for example, ultrafiltration, and saponin flocs are removed with an acid. The present invention proposes a method for purifying beet saponin in which the flocs are formed, dissolved in an alkaline aqueous solution, and treated by adding an organic solvent and activated carbon.
【００１３】ここで、サポニン抽出時における水溶液の
ｐＨは中性でもよいが、好ましくはｐＨ10〜12であっ
て、この場合のアルカリ調整剤としては水酸化ナトリウ
ム、水酸化カリウム、炭酸ナトリウム、炭酸水素ナトリ
ウム、アンモニア等を使用することができる。Here, the pH of the aqueous solution at the time of saponin extraction may be neutral, but it is preferably pH 10 to 12, and in this case, the alkali adjusting agent is sodium hydroxide, potassium hydroxide, sodium carbonate or hydrogen carbonate. Sodium, ammonia and the like can be used.
【００１４】また、ペクチン質等の高分子成分を除去す
るために使用する限外濾過膜としては、分子量２万以上
のものをカットできるポアサイズの大きさ0.2 μm のも
のまで使用できるが、サポニン回収率の面からは、分子
量２万カット前後の膜を使用することが好ましい。As the ultrafiltration membrane used for removing high molecular components such as pectin, up to 0.2 μm in pore size capable of cutting a molecular weight of 20,000 or more can be used, but saponin recovery is possible. From the viewpoint of the rate, it is preferable to use a film having a molecular weight of about 20,000 cut.
【００１５】限外濾過処理後、透過液中のサポニンのフ
ロックを形成するために使用する酸としては塩酸、硫
酸、硝酸及び有機酸等を使用することができ、そのｐＨ
は１〜４、好ましくは1.5 前後である。After the ultrafiltration treatment, hydrochloric acid, sulfuric acid, nitric acid, organic acids and the like can be used as the acid used for forming the flocs of the saponin in the permeate, and the pH thereof can be used.
Is 1 to 4, preferably around 1.5.
【００１６】このフロックを、例えば遠心分離により採
取し、適量なアルカリ水溶液に溶解させて、サポニン水
溶液を調整し、これに有機溶媒及び活性炭を加える。The flocs are collected, for example, by centrifugation and dissolved in an appropriate amount of an alkaline aqueous solution to prepare a saponin aqueous solution, to which an organic solvent and activated carbon are added.
【００１７】ここで、アルカリ水溶液としては水酸化ナ
トリウム、水酸化カリウム、炭酸ナトリウム、炭酸水素
ナトリウム、アンモニア等のアルカリ水溶液が使用され
る。なお、フロックを溶解したサポニン水溶液の固形分
濃度は１〜20% 、好ましくは10〜30% である。Here, as the alkaline aqueous solution, an alkaline aqueous solution of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogen carbonate, ammonia or the like is used. The solid content concentration of the saponin solution in which flocs are dissolved is 1 to 20%, preferably 10 to 30%.
【００１８】サポニン水溶液には例えば有機溶媒を混合
撹拌後、活性炭を添加して１〜２時間反応させる。For example, an organic solvent is mixed and stirred in the saponin aqueous solution, activated carbon is added, and the reaction is carried out for 1 to 2 hours.
【００１９】ここで有機溶媒としては、水に溶解し易い
極性の比較的大きい有機溶媒、例えばメタノール、エタ
ノール、アセトン、ピリジン、ジオキサン、フェノール
等の１種又は２種以上を使用できるが、好ましくはメタ
ノール、エタノールである。Here, as the organic solvent, an organic solvent having a relatively large polarity which is easily dissolved in water, for example, one or more of methanol, ethanol, acetone, pyridine, dioxane, phenol and the like can be used, but it is preferable. They are methanol and ethanol.
【００２０】活性炭としては、粉末活性炭が好ましい
が、粒状活性炭も使用でき、特に形状にこだわるもので
はない。As the activated carbon, powdered activated carbon is preferable, but granular activated carbon can also be used and is not particularly limited in shape.
【００２１】反応温度は室温でよいが、加温して反応時
間を短縮することもできる。The reaction temperature may be room temperature, but the reaction time can be shortened by heating.
【００２２】反応後、濾過機等適当な手段により活性
炭、及び吸着不純物を除去し、得られた精製液を減圧留
去により、水、溶媒を除去し、乾燥することにより精製
サポニンを得る。After the reaction, activated carbon and adsorbed impurities are removed by an appropriate means such as a filter, and the obtained purified liquid is distilled off under reduced pressure to remove water and solvent and dried to obtain purified saponin.
【００２３】この精製方法により脱色率は90〜95% にも
達し、白色の粉末が得られ、純度も若干の向上が可能と
なった。By this purification method, the decolorization rate reached 90 to 95%, a white powder was obtained, and the purity could be slightly improved.
【００２４】本願の第２発明は、第１発明で得られたサ
ポニンの純度及び回収率を更に向上させるためのもので
あり、第１発明で得られたサポニン水溶液を濃縮し、乾
燥した粗サポニンを適当量の有機溶媒に溶解し、不溶性
不純物を濾過等により除去し、濾液に活性炭を加えて精
製し、得られた精製液の溶媒を減圧留去させて純度の高
いサポニンを得る方法である。The second invention of the present application is to further improve the purity and recovery rate of the saponin obtained in the first invention, and the crude saponin obtained by concentrating and drying the saponin aqueous solution obtained in the first invention. Is dissolved in an appropriate amount of an organic solvent, insoluble impurities are removed by filtration, etc., activated carbon is added to the filtrate for purification, and the solvent of the resulting purified solution is distilled off under reduced pressure to obtain a highly pure saponin. .
【００２５】ここで有機溶媒としては、上記同様に水に
溶解し易い極性の比較的大きい有機溶媒が使用できる
が、メタノール、エタノール、アセトン、ピリジンが適
しており、これらの溶媒は必要に応じて混合して使用す
ることができる。As the organic solvent, an organic solvent having a relatively large polarity which is easily dissolved in water can be used as described above, but methanol, ethanol, acetone and pyridine are suitable, and these solvents are used as necessary. It can be mixed and used.
【００２６】有機溶媒の必要量としては、メタノールの
場合、粗サポニン１部当たり、30〜200 部であり、好ま
しくは50〜100 部で、不溶性不純物の除去には十分であ
り、他の有機溶媒を使用する場合、適時使用量を増減さ
せればよい。In the case of methanol, the required amount of the organic solvent is 30 to 200 parts, preferably 50 to 100 parts, per 1 part of the crude saponin, which is sufficient for removing insoluble impurities. When using, the amount used may be increased or decreased at appropriate times.
【００２７】反応温度は第１発明と同様に室温でよい
が、加温して反応時間を短縮することができる。The reaction temperature may be room temperature as in the first invention, but the reaction time can be shortened by heating.
【００２８】以上、有機溶媒の系に活性炭を添加するこ
とにより、活性炭にサポニンを吸着させずに脱色が可能
となり、色調の改善されたサポニン製造が可能となる。As described above, by adding activated carbon to the organic solvent system, decolorization is possible without adsorbing saponin on activated carbon, and saponin with improved color tone can be produced.
【００２９】[0029]
【発明の効果】即ち、本願第１発明によれば特開平4-10
3598号で提案された方法による純度、回収率を低下させ
ずに色調の改善された製品を製造できる。That is, according to the first invention of the present application, Japanese Patent Laid-Open No. 4-10
A product with improved color tone can be produced without lowering the purity and recovery rate by the method proposed in 3598.
【００３０】本願第２発明によれば第１発明と同様に色
調が改善され、且つ純度、回収率が更に改善される精製
方法であり、天然界面活性剤として広範な用途が期待で
きる。According to the second invention of the present application, it is a refining method in which the color tone is improved and the purity and recovery rate are further improved as in the first invention, and a wide range of uses as a natural surfactant can be expected.
【００３１】[0031]
【実施例】以下、この発明の実施例を示す。 実施例１ プレスドパルプ20Kgに水100リットル を加え、25% 水酸化ナ
トリウム溶液にてｐＨ10とし、50℃に加温しながら３時
間撹拌抽出し、固液分離して抽出液を得た。EXAMPLES Examples of the present invention will be shown below. Example 1 To 20 kg of pressed pulp, 100 liters of water was added, the pH was adjusted to 10 with 25% sodium hydroxide solution, and the mixture was extracted with stirring for 3 hours while heating at 50 ° C, and solid-liquid separation was performed to obtain an extract.
【００３２】この抽出液を２万分画の限外濾過膜にて処
理し、この透過液に35% 塩酸を添加してｐＨ1.5 とし、
30分間静置してサポニンフロックを生成させた後、遠心
分離によりフロック480gを得た。The extract was treated with an ultrafiltration membrane of 20,000 fractions, and 35% hydrochloric acid was added to the permeate to adjust the pH to 1.5,
After leaving still for 30 minutes to generate saponin flocs, centrifugation was performed to obtain 480 g of flocs.
【００３３】次いで、このフロックを５％水酸化ナトリ
ウム溶液にて中和溶解し、粗サポニン水溶液1リットル （固
形分当たりのサポニン純度35%、サポニン含量24.5g)を得
た。Next, the flocs were neutralized and dissolved in a 5% sodium hydroxide solution to obtain 1 liter of a crude saponin aqueous solution (saponin purity 35% per solid content, saponin content 24.5 g).
【００３４】このサポニン水溶液にメタノール500ml を
混合し、粉末活性炭10g を添加し、１時間室温にて撹拌
反応させた。500 ml of methanol was mixed with this saponin aqueous solution, 10 g of powdered activated carbon was added, and the mixture was reacted with stirring at room temperature for 1 hour.
【００３５】次に、不溶性不純物及び活性炭吸着不純物
を濾別し、得られた精製液の水−メタノール混合物をロ
ータリーエバポレーターにより減圧留去し、更に残留物
を真空低温乾燥して白色の精製ビートサポニン36.9g
（サポニン純度60.5%、サポニン含量22.34g) を得た。本
例によるサポニン回収率は91% である。Next, insoluble impurities and activated carbon adsorbed impurities were filtered off, the water-methanol mixture of the obtained purified liquid was distilled off under reduced pressure by a rotary evaporator, and the residue was vacuum dried at low temperature to give white purified beet saponin. 36.9g
(Saponin purity 60.5%, saponin content 22.34 g) was obtained. The saponin recovery rate in this example is 91%.
【００３６】実施例２ プレスドパルプ27g に水135リットル を加え、実施例１と同
様に処理し、サポニンフロック670gを得た。得られたフ
ロックを５％水酸化ナトリウム溶液にて中和溶解し、ロ
ータリーエバポレーターで濃縮後、60℃の送風乾燥機で
乾燥して粗サポニン100g（サポニン純度37% 、サポニン
含量37g ）を得た。Example 2 135 g of water was added to 27 g of pressed pulp and treated in the same manner as in Example 1 to obtain 670 g of saponin floc. The obtained flocs were neutralized and dissolved in a 5% sodium hydroxide solution, concentrated with a rotary evaporator, and then dried with a blow dryer at 60 ° C. to obtain 100 g of crude saponin (purity of saponin 37%, saponin content 37 g). .
【００３７】この粗サポニン100gにメタノール 8リットルを
加え、１時間室温にて撹拌溶解した後、不溶性不純物を
濾別し、得られた濾液に粉末活性炭14g を加え、１時間
室温にて反応させた後、活性炭及び不純物を濾別する。To 100 g of this crude saponin, 8 liters of methanol was added and dissolved by stirring at room temperature for 1 hour, then insoluble impurities were filtered off, and 14 g of powdered activated carbon was added to the obtained filtrate and reacted at room temperature for 1 hour. After that, activated carbon and impurities are filtered off.
【００３８】この精製液のメタノールをロータリーエバ
ポレーターで減圧留去し、残留物を真空低温乾燥して白
色の精製ビートサポニン55.8g(サポニン純度63%、サポニ
ン含量35.15g) を得た。本例でのサポニン回収率は95%
であった。Methanol of this purified liquid was distilled off under reduced pressure with a rotary evaporator, and the residue was vacuum dried at low temperature to obtain 55.8 g of white purified beet saponin (purity of saponin 63%, saponin content 35.15 g). The saponin recovery rate in this example is 95%
Met.
【００３９】実施例３ 実施例２と同様に処理して得られた粗サポニン50g をア
セトン 5リットルを加え、１時間室温にて撹拌溶解して不溶
性不純物を濾別し、得られた濾液に粉末活性炭6gを加
え、１時間室温にて撹拌反応させた後、活性炭及び吸着
不純物を濾別する。Example 3 50 g of crude saponin obtained by treating in the same manner as in Example 2 was added with 5 L of acetone and dissolved by stirring for 1 hour at room temperature to remove insoluble impurities by filtration, and the resulting filtrate was powdered. After adding 6 g of activated carbon and stirring and reacting at room temperature for 1 hour, activated carbon and adsorbed impurities are separated by filtration.
【００４０】この精製液のアセトンをロータリーエバポ
レーターにより減圧留去し、残留物を真空低温乾燥して
白色の精製ビートサポニン28g （サポニン純度62%、サポ
ニン含量17.36g) を得た。本例でのサポニン回収率は94
% であった。Acetone of this purified liquid was distilled off under reduced pressure by a rotary evaporator, and the residue was vacuum dried at a low temperature to obtain 28 g of white purified beet saponin (saponin purity 62%, saponin content 17.36 g). The saponin recovery rate in this example is 94.
% Met.
【００４１】実施例４ 粗サポニン( 純度35%)を5gづつ計量し、1000mlのメタノ
ールに溶解させた（試験区）。試験区には予備試験
の結果から、活性炭4g添加し、室温にて２時間撹拌して
脱色し、この液を濾紙(No.5C) を使用して濾過後、この
脱色精製液を真空濃縮してから60℃にて乾燥し、この乾
燥物を計量し、サポニンの純度、純粋サポニンの量、回
収率を測定した。Example 4 5 g of crude saponin (purity 35%) was weighed and dissolved in 1000 ml of methanol (test group). Based on the results of the preliminary test, 4 g of activated carbon was added to the test area, and the mixture was stirred at room temperature for 2 hours for decolorization. After filtering this solution using filter paper (No. 5C), the decolorized purified solution was concentrated under vacuum. After that, it was dried at 60 ° C., the dried product was weighed, and the saponin purity, the amount of pure saponin, and the recovery rate were measured.
【００４２】また、色調については、乾燥物の１％水溶
液を作製し、420nm にて吸光度(O.D) を測定し、これよ
り色価及び脱色率を測定した。Regarding the color tone, a 1% aqueous solution of the dried product was prepared, the absorbance (OD) was measured at 420 nm, and the color value and the decolorization rate were measured.
【００４３】比較例 上記同様な粗サポニン( 純度35%)を5gづつ計量し、1000
mlの水に溶解させた（試験区）。試験区には予備試
験の結果から、活性炭をＡ：2.5g、Ｂ：5g、Ｃ：7.5g添
加し、これ以降は実施例４と同様にして脱色精製液から
サポニンの純度、純粋サポニンの量、回収率を測定し、
色調についても、実施例４と同様にして１％水溶液から
色価及び脱色率を測定した。その結果を下記の表２に示
す。Comparative Example Crude saponin (purity 35%) similar to the above was weighed in 5 g increments and 1000 g
It was dissolved in ml of water (test area). Based on the results of the preliminary test, A: 2.5 g, B: 5 g, and C: 7.5 g of activated carbon were added to the test section, and thereafter, in the same manner as in Example 4, the saponin purity and the amount of pure saponin from the decolorized purified solution. , Measure the recovery rate,
Regarding the color tone, the color value and the decolorization rate were measured from a 1% aqueous solution in the same manner as in Example 4. The results are shown in Table 2 below.
【００４４】[0044]
【表２】 [Table 2]
Claims (2)
菜をスライスし浸出装置にて糖を抽出した残渣を水又は
アルカリ水溶液でサポニンを抽出し、得られた抽出液中
の高分子成分を除去し、酸によりサポニンフロックを形
成させ、このフロックをアルカリ水溶液にて溶解し、有
機溶媒及び活性炭を添加処理して精製することを特徴と
するビートサポニンの精製方法。1. A crushed product of sugar beet, a sliced product, or a residue obtained by slicing sugar beet and extracting sugar with a leaching device to extract saponin with water or an alkaline aqueous solution to remove polymer components in the obtained extract. Then, a saponin floc is formed with an acid, the floc is dissolved in an alkaline aqueous solution, and an organic solvent and activated carbon are added and purified to purify the beet saponin.
菜をスライスし浸出装置にて糖を抽出した残渣を水又は
アルカリ水溶液でサポニンを抽出し、得られた抽出液中
の高分子成分を除去し、酸によりサポニンフロックを形
成させ、このフロックをアルカリ水溶液にて溶解して得
られたサポニン水溶液を、濃縮、乾燥してから有機溶媒
に溶解し、不溶性不純物を濾過除去し、その清澄液に活
性炭を添加処理して精製することを特徴とするビートサ
ポニンの精製方法。2. A crushed product, a sliced product of sugar beet, or a residue obtained by slicing sugar beet and extracting sugar with a leaching device to extract saponin with water or an alkaline aqueous solution to remove high molecular components in the obtained extract. Then, saponin flocs are formed with acid, and the saponin aqueous solution obtained by dissolving the flocs in an alkaline aqueous solution is concentrated and dried, and then dissolved in an organic solvent, insoluble impurities are removed by filtration, and the resulting clear solution is obtained. A method for purifying beet saponin, which comprises purifying by adding activated carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5098424A JP2605207B2 (en) | 1993-04-02 | 1993-04-02 | Purification method of beet saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5098424A JP2605207B2 (en) | 1993-04-02 | 1993-04-02 | Purification method of beet saponin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06287199A true JPH06287199A (en) | 1994-10-11 |
| JP2605207B2 JP2605207B2 (en) | 1997-04-30 |
Family
ID=14219434
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5098424A Expired - Fee Related JP2605207B2 (en) | 1993-04-02 | 1993-04-02 | Purification method of beet saponin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2605207B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759283A (en) * | 1996-05-14 | 1998-06-02 | The Western Sugar Company | Method for processing sugar beets to produce a purified beet juice product |
| JP2018523651A (en) * | 2015-07-27 | 2018-08-23 | ミン−ダック ファーマーズ コーペレイティブＭｉｎｎ−Ｄａｋ Ｆａｒｍｅｒｓ Ｃｏｏｐｅｒａｔｉｖｅ | Process for extracting saponin from agricultural products |
| WO2018182762A1 (en) * | 2017-03-31 | 2018-10-04 | Healthpro Brands, Inc. | Method of increasing water soluble nutrient extraction from plant matter |
| CN113150038A (en) * | 2021-03-19 | 2021-07-23 | 南阳理工学院 | Method for purifying gleditsia sinensis saponin through flocculation decoloration, membrane treatment and two-phase extraction |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02160798A (en) * | 1988-12-13 | 1990-06-20 | Maruzen Kasei Co Ltd | Anti-dermatophyte agent |
| JPH04103598A (en) * | 1990-08-24 | 1992-04-06 | Hokkaido Togyo Kk | Production of beet saponin |
-
1993
- 1993-04-02 JP JP5098424A patent/JP2605207B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02160798A (en) * | 1988-12-13 | 1990-06-20 | Maruzen Kasei Co Ltd | Anti-dermatophyte agent |
| JPH04103598A (en) * | 1990-08-24 | 1992-04-06 | Hokkaido Togyo Kk | Production of beet saponin |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5759283A (en) * | 1996-05-14 | 1998-06-02 | The Western Sugar Company | Method for processing sugar beets to produce a purified beet juice product |
| JP2018523651A (en) * | 2015-07-27 | 2018-08-23 | ミン−ダック ファーマーズ コーペレイティブＭｉｎｎ−Ｄａｋ Ｆａｒｍｅｒｓ Ｃｏｏｐｅｒａｔｉｖｅ | Process for extracting saponin from agricultural products |
| WO2018182762A1 (en) * | 2017-03-31 | 2018-10-04 | Healthpro Brands, Inc. | Method of increasing water soluble nutrient extraction from plant matter |
| US10465256B2 (en) | 2017-03-31 | 2019-11-05 | Healthpro Brands, Inc. | Method of increasing water soluble nutrient extraction from plant matter |
| CN113150038A (en) * | 2021-03-19 | 2021-07-23 | 南阳理工学院 | Method for purifying gleditsia sinensis saponin through flocculation decoloration, membrane treatment and two-phase extraction |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2605207B2 (en) | 1997-04-30 |
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