JPH06227930A - Agent for controlling injurious organisms against paddy rice - Google Patents

Abstract

PURPOSE:To provide an injurious organism-controlling agent capable of effectively controlling paddy rice-injuring organisms causing rice blast, rice sheath blight, rice NAIEIKAPPENBYO (caused by Erwinia herbicola), effectively on its usage in low concentrations, and minimized in chemical injury. CONSTITUTION:The rice blast, rice sheath blight or NAIEIKAPPENBYO- controlling agent is obtained by compounding 0.25-4 pts.wt. of acetic acid with 1 pt.wt. of a chitosan decomposition product, and a method for controlling rice injurious organisms comprises using the paddy rice-injuring organism- controlling agent.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、キトサン分解物と酢酸
とをそれぞれ所定の割合で配合してなる水稲の有害生物
防除剤、該防除剤にアルファルファ緑葉脱蛋白質上澄液
を配合してなる水稲の有害生物防除剤、並びにこれら防
除剤を用いた水稲有害生物の防除方法に関する。FIELD OF THE INVENTION The present invention relates to a pest control agent for paddy rice, which comprises a decomposed product of chitosan and acetic acid in predetermined proportions, and a control solution containing alfalfa green leaf deproteinized supernatant. TECHNICAL FIELD The present invention relates to pest control agents for paddy rice and a method for controlling paddy rice pests using these control agents.

【０００２】[0002]

【従来の技術及び発明が解決しようとする課題】キトサ
ンは、エビ，カニ等の甲殻や微生物の細胞壁などに含ま
れるキチンを脱アセチル化したものであり、キトサンを
分解して得られる低重合度のもの（以下、「キトサン分
解物」という。）も知られている。キトサンやキトサン
分解物が、植物病原性のカビの生育に影響を及ぼすこと
〔シー・アール・アラン他：エクスペリメンタル・マイ
コロジー（ C. R. Allen et al：Experimental Mycolog
y ）、第３巻、第２８５〜２８７頁( 1979年）、ピー・
エス・ストエッセル他：フィトパソロギッシェ・ツァイ
トシュリフト( P. S. Stoessel et al：Phytopathologi
sche Zeitschrift )第 111巻、第８２〜８９頁( 1984
年）、ディー・エフ・ケンドラ他：エクスペリメンタル
・マイコロジー( D. F. Kendra et al: Experimental M
ycology ）、第８巻、第２７６〜２８１頁(1984 年）、
平野他：アグリカルチュアル アンド バイオロジカル
ケミストリー( Agricultural and Biological Chemis
try ）、第５３巻、第 3065 〜 3066 頁( 1989 年) 〕
が知られており、実用面では梨黒斑病の防除剤（特開昭
６２−１９８６０４号公報）が提案されている。BACKGROUND OF THE INVENTION Chitosan is deacetylated chitin contained in the shells of shrimp, crab, etc. and cell walls of microorganisms, and has a low degree of polymerization obtained by degrading chitosan. The thing (henceforth "the chitosan decomposition product") is also known. Effects of chitosan and chitosan degradation products on the growth of phytopathogenic fungi [CR Allen et al: Experimental Mycolog
y), Volume 3, pp. 285-287 (1979), P.
S. Stoessel et al: Phytopathologi
sche Zeitschrift) Vol. 111, pp. 82-89 (1984
), DF Kendra et al .: Experimental Mycology (DF Kendra et al: Experimental M
ycology), Vol. 8, 276-281 (1984),
Hirano et al .: Agricultural and Biological Chemis
try), Vol. 53, pp. 3065-3066 (1989)]
Is known, and a pesticidal disease control agent (Japanese Unexamined Patent Publication No. 62-198604) has been proposed in practical use.

【０００３】しかし、これらの植物有害生物防除剤は、
高濃度で用いると薬害が出ること等の問題があり、改良
の余地が残されている。そのため、低濃度の使用で有効
であって、抗菌スペクトルが広い、薬害のない植物有害
生物防除剤の開発が望まれている。また、酢酸等の有機
酸類が殺菌作用を有していること及びその殺菌作用に着
目して、有機酸類を有効成分として殺菌剤に供すること
が知られている。とりわけ、植物体に対する場合に比
し、薬害が比較的問題とならない食品分野において、食
品用殺菌剤，静菌剤として有機酸類を使用することが既
に実施されている。However, these plant pest control agents are
There is a problem such as chemical damage when used at a high concentration, and there is room for improvement. Therefore, it is desired to develop a plant pest control agent that is effective at low concentrations and has a broad antibacterial spectrum and is free from phytotoxicity. Further, it is known that organic acids such as acetic acid have a bactericidal action and that the organic acids are used as an active ingredient in a bactericidal agent, focusing on the bactericidal action. In particular, in the field of foods where phytotoxicity is relatively less problematic than in the case of plants, it has already been practiced to use organic acids as fungicides and bacteriostats for foods.

【０００４】しかしながら、これらの殺菌剤等の場合、
有効成分としての有機酸類の濃度を高濃度に維持するこ
とが必要不可欠の条件である。有機酸類を殺菌作用を呈
するような高濃度で植物体に対して適用すると、顕著な
薬害が生ずることから、このような状態での実施は不可
能である。有機酸類の中でも特に強い薬害を示す酢酸等
を、その殺菌作用を維持させた高い濃度で、薬害耐性の
弱い植物体に直接散布することは到底不可能のことであ
る。However, in the case of these germicides and the like,
Maintaining a high concentration of organic acids as an active ingredient is an essential condition. When organic acids are applied to plants at such a high concentration that they exhibit a bactericidal action, remarkable phytotoxicity occurs, and therefore it is impossible to carry out in such a state. It is impossible at all to directly spray acetic acid and the like, which shows particularly strong phytotoxicity among organic acids, to a plant having weak resistance to phytotoxicity at a high concentration while maintaining its bactericidal action.

【０００５】一方、アルファルファ緑葉脱蛋白質上澄液
（以下、「ブラウン・ジュース」という。）の植物への
利用に関しては、これまでに肥料等としての利用が試み
られているが、まだ確立されておらず、ましてや水稲の
有害生物防除剤としての利用については全く検討されて
いない。On the other hand, regarding the use of the alfalfa green leaf deproteinized supernatant (hereinafter referred to as "Brown juice") in plants, it has been attempted to be used as a fertilizer, etc., but it has not been established yet. Not to mention the use of paddy rice as a pest control agent.

【０００６】[0006]

【課題を解決するための手段】そこで本発明者らは、上
記課題を解決すべく鋭意検討を重ねた結果、キトサン分
解物と酢酸とをそれぞれ特定の割合で配合した組成物、
及び該組成物にブラウン・ジュースを添加したものが、
水稲の有害生物防除剤として有効に利用できることを見
出し、かかる知見に基づいて本発明を完成するに至った
のである。すなわち本発明は、キトサン分解物１重量部
あたり、酢酸を0.２５〜４重量部の割合で配合してなる
組成物、或いは該組成物にブラウン・ジュースを添加し
たものを、水稲のいもち病、紋枯病、内穎褐変病防除剤
等とする水稲の有害生物防除剤であり、さらに該水稲有
害生物防除剤を用いた水稲有害生物の防除方法である。Therefore, as a result of intensive studies to solve the above problems, the inventors of the present invention have found that a composition in which a decomposed product of chitosan and acetic acid are mixed in specific proportions,
And the addition of brown juice to the composition,
They have found that they can be effectively used as pest control agents for paddy rice, and have completed the present invention based on such findings. That is, the present invention relates to a rice blast disease of paddy rice, which comprises a composition comprising 0.25 to 4 parts by weight of acetic acid per 1 part by weight of a decomposed product of chitosan, or a composition obtained by adding brown juice to the composition. The present invention also relates to a pest control agent for paddy rice, such as a control agent for rice blight, browning disease of inner sponge, and the like, and a method for controlling pests of paddy rice using the pest control agent for paddy rice.

【０００７】本発明に用いるキトサン分解物は、前述の
キトサンを酸や酵素などを用いて、適当な分子量に分解
したものである。ここでキトサンの酸分解に用いる酸と
しては、有機酸，無機酸のいずれでもあってもよい。有
機酸としては、例えば酢酸，乳酸，クエン酸，リンゴ酸
等があり、無機酸としては、例えば塩酸，硫酸等があ
る。また、酵素分解に使用する酵素としては、キトサナ
ーゼ，パパイン，セルラーゼ，酸性プロテアーゼ，ペク
チナーゼなどがある。The decomposed product of chitosan used in the present invention is obtained by decomposing the above-mentioned chitosan into an appropriate molecular weight using an acid or an enzyme. The acid used for the acid decomposition of chitosan may be either an organic acid or an inorganic acid. Examples of the organic acid include acetic acid, lactic acid, citric acid, malic acid and the like, and examples of the inorganic acid include hydrochloric acid, sulfuric acid and the like. In addition, examples of enzymes used for enzymatic decomposition include chitosanase, papain, cellulase, acid protease, pectinase and the like.

【０００８】なお、キトサンの酸分解法については、特
開平1-128775号公報等に例示されており、またキトサン
の酵素分解については、特開平１−291799号公報等に例
示されている。これらの方法により得られたキトサン分
解物を本発明に使用することができる。キトサンの分解
の程度については特に制限されないが、分子量10000〜5
0000 程度のものが抗菌性が強く（特開平１−128775号
公報参照）、好適である。The acid decomposition method of chitosan is illustrated in JP-A 1-128775 and the like, and the enzymatic decomposition of chitosan is illustrated in JP-A-1-291799 and the like. Chitosan degradation products obtained by these methods can be used in the present invention. The degree of decomposition of chitosan is not particularly limited, but the molecular weight is 10,000 to 5
Those of about 0000 have strong antibacterial properties (see JP-A-1-128775) and are preferable.

【０００９】次に、本発明に使用する酢酸としては、合
成酢酸の他、酢酸含有液，食酢等がある。ここで酢酸含
有液の１例として、特許第 959222 号明細書に記載され
ている食酢溶液がある。食酢溶液は酢酸の他に酢酸塩を
含んでおり、合成酢酸や食酢に比べ、薬害を生ずること
が少ないという特色を有している。The acetic acid used in the present invention includes synthetic acetic acid, acetic acid-containing liquid, vinegar and the like. Here, as one example of the acetic acid-containing liquid, there is an vinegar solution described in Japanese Patent No. 959222. The vinegar solution contains acetic acid salt in addition to acetic acid, and has the feature that it causes less chemical damage than synthetic acetic acid or vinegar.

【００１０】また、本発明に用いるブラウン・ジュース
（アルファルファ緑葉脱蛋白質上澄液）は、飼料や食品
として利用されているアルファルファから蛋白質を加熱
除去したものであり、その製造法の１例は特開平３−５
８９０５号公報及び特開平４−５４１０５号公報に記載
されている。Further, the brown juice (alfalfa green leaf deproteinized supernatant) used in the present invention is obtained by heating and removing proteins from alfalfa which is used as a feed or food, and one example of its production method is special. Kaihei 3-5
It is described in JP-A-8905 and JP-A-4-54105.

【００１１】本発明では、抗菌作用のあるキトサン分解
物と酢酸（合成酢酸，酢酸含有液，食酢等）とを、前記
したように、それぞれ特定の割合で混合して用いると、
多種の水稲有害生物に対する強い相乗的な抗菌作用が発
現する。そのため、薬害の生じない低濃度で有効であ
り、かつ抗菌スペクトルの広い水稲有害生物防除剤の提
供が可能となった。水稲の有害生物に対する防除効果
は、上記２成分（キトサン分解物と酢酸）に対し、第３
成分としてブラウン・ジュースを配合することにより、
薬害軽減効果が顕著となり、高濃度での薬剤防除処理が
可能となるため、防除効果が一層高まる。ここで、ブラ
ウン・ジュースの配合量については、特に制限はない
が、通常第３成分を含めた総重量の２〜２０％程度にな
るように混合すればよい。In the present invention, when chitosan degradation product having an antibacterial action and acetic acid (synthetic acetic acid, acetic acid-containing liquid, vinegar, etc.) are mixed in a specific ratio as described above,
A strong synergistic antibacterial action against various rice pests is developed. Therefore, it became possible to provide a paddy rice pest control agent which is effective at a low concentration without causing chemical damage and has a broad antibacterial spectrum. The control effect of paddy rice against pests is 3rd higher than the above 2 components (chitosan degradation product and acetic acid).
By blending brown juice as an ingredient,
The phytotoxicity-reducing effect becomes remarkable, and the chemical control treatment can be performed at a high concentration, so that the control effect is further enhanced. Here, the amount of the brown juice is not particularly limited, but usually, it may be mixed so as to be about 2 to 20% of the total weight including the third component.

【００１２】本発明の水稲有害生物防除剤は、適当な担
体等を適宜加えて、液剤，粉剤，粒剤等の形態で使用さ
れる。本発明の水稲有害生物防除剤の使用方法，使用濃
度等は、病害の種類，その他の要因により様々である
が、例えば液剤の場合には、酢酸の濃度が0.００２〜0.
２重量％程度となるように配合し、水稲に散布する。散
布間隔は通常、５〜２０日毎とし、防除効果を観察しな
がら決定すればよい。The pest control agent for paddy rice of the present invention is used in the form of a liquid, powder, granule or the like by appropriately adding a suitable carrier and the like. The use method, use concentration and the like of the paddy rice pest control agent of the present invention vary depending on the type of disease and other factors. For example, in the case of a liquid agent, the acetic acid concentration is 0.002 to 0.
Mix it to about 2% by weight and spray on paddy rice. The spraying interval is usually every 5 to 20 days and may be determined while observing the control effect.

【００１３】本発明の水稲有害生物防除剤は、使用に際
して毒性を問題にする必要がないので、散布時期は育苗
期，生育期のみならず、収穫期直前にも使用することが
できる。Since the paddy rice pest control agent of the present invention does not need to cause toxicity when used, it can be used not only at the seedling raising and growing seasons but also immediately before the harvesting season.

【００１４】[0014]

【実施例】以下に実験例及び実施例を挙げて本発明を具
体的に説明するが、本発明はこれらにより限定されるも
のではない。 実験例１ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）２重量％を３％(W
/V）の酢酸溶液に溶解しキトサン溶液とし、１２１℃で
６０分間加圧加熱処理し、加水分解を行った。次いで、
水酸化ナトリウムを用いて、 pH 5.０に調整し、キトサ
ン分解物(A) を得た。EXAMPLES The present invention will be specifically described below with reference to experimental examples and examples, but the present invention is not limited thereto. Experimental Example 1 (1) Preparation of chitosan degradation product 2% by weight of chitosan (manufactured by Tokyo Kasei Kogyo Co., Ltd.) to 3% (W
/ V) was dissolved in an acetic acid solution to give a chitosan solution, which was hydrolyzed by heating under pressure at 121 ° C for 60 minutes. Then
The pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan decomposition product (A).

【００１５】（２）キトサン分解物と酢酸を含有する培
地の調製 ＰＳ培地（ポテト２００ｇ，ショ糖２０ｇ，蒸留水１リ
ットル，pH 5.0 ）に、前記（１）で得たキトサン分解
物(A）と合成酢酸の合計重量％が0.０１％（キトサン分
解物(A）の場合は原料キトサン濃度として換算）となる
ように、種々の混合比で添加した培地（ pH 5.０に調
整）を調製し、各々試験管に１０mlずつ分注し、１２１
℃で１５分間滅菌した。(2) Preparation of medium containing chitosan degradation product and acetic acid In PS medium (potato 200 g, sucrose 20 g, distilled water 1 liter, pH 5.0), the chitosan degradation product (A) obtained in the above (1) was added. So that the total weight% of synthetic and acetic acid is 0.01% (in the case of chitosan degradation product (A), it is converted as the concentration of raw material chitosan), the medium added at various mixing ratios (adjusted to pH 5.0) Prepare and dispense 10 ml into each test tube.
Sterilized for 15 minutes at ° C.

【００１６】（３）抗菌性試験 上記（２）で得られた培地に、イネ内穎褐変病菌である
エルビニア・ハービコーラ（ Erwinia herbicola ) I
FO 12686 のＰＳ培地での３０℃，２４時間振とう培養
液を 0. ０５ml接種し、３０℃で１５６時間振とう培養
した。培養後、６６０nmの吸光度を測定し、供試菌株の
生育量を相対比較した。結果を第１表に示す。第１表か
ら明らかなように、キトサン分解物(A) と酢酸との重量
混合比が１対0.２５〜４のときに供試菌株に対する強い
相乗的抗菌作用が発現した。[0016] (3) to the medium obtained in the antibacterial property test above (2), rice inside穎褐a strange fungi Erwinia Habikora (Erwinia herbicola) I
0.05 ml of a FO 12686 PS medium shaking culture at 30 ° C. for 24 hours was inoculated and shaking culture was performed at 30 ° C. for 156 hours. After culturing, the absorbance at 660 nm was measured, and the growth amounts of the test strains were compared with each other. The results are shown in Table 1. As is clear from Table 1, a strong synergistic antibacterial action against the test strain was exhibited when the weight mixing ratio of the chitosan degradation product (A) and acetic acid was 1: 0.25 to 4.

【００１７】[0017]

【表１】 [Table 1]

【００１８】実験例２ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）２重量％を0.２Ｎ
塩酸に混合溶解しキトサン溶液とし、１００℃で１２０
分間加熱処理し、加水分解を行った。次いで、水酸化ナ
トリウムを用いて、 pH 5.０に調整し、キトサン分解物
(B) を得た。Experimental Example 2 (1) Preparation of Decomposed Product of Chitosan 2% by weight of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 0.2N.
Mix and dissolve in hydrochloric acid to make a chitosan solution, 120 at 100 ℃
After heat treatment for a minute, hydrolysis was performed. Then, adjust the pH to 5.0 with sodium hydroxide, and decompose the chitosan
I got (B).

【００１９】（２）キトサン分解物と食酢と含有する培
地の調製 ＰＳＡ培地（ポテト２００ｇ，ショ糖２０ｇ，寒天２０
ｇ，蒸留水１リットル，pH 5. ０）に、前記（１）で得
たキトサン分解物(B) と酢酸濃度１０重量％の醸造酢
を、キトサン分解物(B) と酢酸との合計重量％が0.05％
（キトサン分解物(B) の場合は原料キトサン濃度として
換算）となるように、種々の混合比で添加した培地（ p
H 5.０に調整）を調製し、各々試験管に１０mlずつ分注
し、１２１℃で１５分間滅菌後、斜面培地とした。(2) Preparation of medium containing chitosan degradation product and vinegar PSA medium (potato 200 g, sucrose 20 g, agar 20)
g, distilled water 1 liter, pH 5.0), the chitosan decomposition product (B) obtained in (1) and brewed vinegar with an acetic acid concentration of 10% by weight, and the total weight of the chitosan decomposition product (B) and acetic acid. % Is 0.05%
(In the case of chitosan degradation product (B), it is converted as the raw material chitosan concentration)
H 5.0) was prepared and 10 ml was dispensed into each test tube, sterilized at 121 ° C. for 15 minutes, and then used as a slant medium.

【００２０】（３）抗菌性試験 上記（２）で得られた培地に、イネ紋枯病菌であるリゾ
クトニア・ソラニ（ Rhizoctonia solani ) IFO 304
64 のＰＳＡ培地での２５℃、５日間培養菌体を接種
し、２５℃で１０日間培養した。培養後、肉眼的に供試
菌株の生育量を相対比較した。結果を第２表に示した。
第２表から明らかなように、キトサン分解物(B) と醸造
酢中の酢酸との重量混合比が１対 0. ４３〜2.３のとき
に供試菌株に対する強い相乗的抗菌作用が発現した。(3) Antibacterial test In the medium obtained in (2) above, Rhizoctonia solani IFO 304 which is a rice blight fungus is used.
64 cells of PSA medium were inoculated at 25 ° C for 5 days, and cultured at 25 ° C for 10 days. After culturing, the growth amounts of the test strains were visually compared relative to each other. The results are shown in Table 2.
As is clear from Table 2, when the weight mixing ratio of the chitosan degradation product (B) and the acetic acid in the brewed vinegar is 1: 0.43 to 2.3, a strong synergistic antibacterial action against the test strain is developed. did.

【００２１】[0021]

【表２】 [Table 2]

【００２２】実施例１ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）５重量％を２％(
W/V ) 乳酸液１００mlに溶解した。次いで、酵素製剤マ
セロチーム２Ｓ（（株）ヤクルト本社製）0.２ｇを加
え、４５℃恒温水槽中で６時間ゆっくり攪拌しながら反
応せしめた。反応終了後、沸騰水中に５分間保ち、酵素
を失活せしめた。しかる後、水酸化ナトリウムを用い
て、 pH 5.０に調整し、キトサン分解物(C) を得た。Example 1 (1) Preparation of Decomposed Product of Chitosan 5% by weight of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 2% (
W / V) It was dissolved in 100 ml of lactic acid solution. Next, 0.2 g of the enzyme preparation Maceroteam 2S (manufactured by Yakult Honsha Co., Ltd.) was added, and the mixture was reacted in a constant temperature water bath at 45 ° C. for 6 hours while slowly stirring. After completion of the reaction, the enzyme was inactivated by keeping it in boiling water for 5 minutes. Thereafter, the pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan decomposition product (C).

【００２３】（２）酢酸含有液の調製 酢酸濃度１０重量％の醸造酢１リットルに対して、卵殻
３０ｇ，重炭酸ソーダ１4.０ｇ及び苛性ソーダ6.６７ｇ
を加えて混合した。この酢酸含有液の酢酸濃度は５重量
％である。(2) Preparation of acetic acid-containing liquid 30 g of eggshell, 14.0 g of sodium bicarbonate and 6.67 g of caustic soda are added to 1 liter of brewed vinegar having an acetic acid concentration of 10% by weight.
Was added and mixed. The acetic acid concentration of this acetic acid-containing liquid is 5% by weight.

【００２４】（３）ブラウン・ジュースの調製 刈り取ったアルファルファを搾汁機にて搾汁してグリー
ン・ジュースを得、これに１３０℃の温度の蒸気を送入
してタンパク質を凝固せしめた後、遠心分離を行って、
目的とするブラウン・ジュースを得た。(3) Preparation of Brown Juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at a temperature of 130 ° C. is fed into the juice to coagulate the protein. Centrifuge,
I got the target brown juice.

【００２５】（４）本発明の水稲有害生物防除剤の調製 前記（１）で得られたキトサン分解物(C) ２重量部と、
前記（２）で得られた酢酸含有液３重量部と、純水0.５
重量部とを混合し、水稲有害生物防除剤(A) を得た。ま
た、上記純水の代わりに、前記（３）で得られたブラウ
ン・ジュースを混合して水稲有害生物防除剤(B) を得
た。(4) Preparation of Paddy Rice Pest Control Agent of the Present Invention 2 parts by weight of the chitosan degradation product (C) obtained in (1) above,
3 parts by weight of the acetic acid-containing solution obtained in (2) above and 0.5% pure water
By mixing with 1 part by weight, a paddy rice pest control agent (A) was obtained. Further, instead of the pure water, the brown juice obtained in the above (3) was mixed to obtain a paddy rice pest control agent (B).

【００２６】（５）水稲有害生物防除剤を含有する培地
の調製 ＰＳＡ培地に、前記（４）で得られた水稲有害生物防除
剤(A）又は(B）を所定濃度（重量％）添加した培地（ p
H 5.０に調整）を調製し、各々試験官に１０ml分注し、
１２１℃で１５分間滅菌後、斜面培地とした。(5) Preparation of Medium Containing Paddy Rice Pest Control Agent The PSA medium was added with the paddy rice pest control agent (A) or (B) obtained in (4) above at a predetermined concentration (% by weight). Medium (p
H adjusted to 5.0) and dispensed 10 ml to each examiner,
After sterilization at 121 ° C. for 15 minutes, it was used as a slant medium.

【００２７】（６）抗菌性試験 前記（５）で得られた抗菌性試験用培地に、植物病原真
菌の場合はＰＳＡ培地での２５℃、５日間培養菌体、植
物病原細菌の場合は同培地での３０℃、２４時間培養菌
体を接種し、植物病原真菌の場合は２５℃で１０日間、
植物病原細菌の場合は３０℃で１０日間培養した。培養
後、肉眼で供試菌株の生育の有無を観察し、最小生育阻
止濃度（ＭＩＣ）を求めた。なお、供試菌株とそれによ
り発生する主な病害は第３表に示す通りである。(6) Antibacterial test The antibacterial test medium obtained in the above (5) is the same as that for the plant pathogenic fungus at 25 ° C. for 5 days in PSA medium and the same for the plant pathogenic bacteria. Inoculated with the cultured cells in the medium at 30 ℃ for 24 hours, and in the case of phytopathogenic fungi, at 25 ℃ for 10 days,
In the case of phytopathogenic bacteria, it was cultured at 30 ° C for 10 days. After the culture, the presence or absence of growth of the test strain was visually observed to determine the minimum inhibitory concentration (MIC). The test strains and the major diseases caused by them are shown in Table 3.

【００２８】[0028]

【表３】 [Table 3]

【００２９】結果を第４表に示したが、本発明の水稲有
害生物防除剤(A）及び(B）は、抗菌スペクトルが極めて
広く、供試した全ての植物病原真菌及び植物病原細菌に
対して、強い抗菌作用を示した。なお、ブラウン・ジュ
ースの添加は抗菌作用に全く影響しなかったが、後述す
るように植物体に対する薬害を軽減する効果がある。The results are shown in Table 4. The rice pest control agents (A) and (B) of the present invention have an extremely broad antibacterial spectrum and are effective against all plant pathogenic fungi and plant pathogenic bacteria tested. And showed a strong antibacterial action. The addition of brown juice did not affect the antibacterial action at all, but it has an effect of reducing phytotoxicity against plants as described later.

【００３０】[0030]

【表４】 [Table 4]

【００３１】実施例２ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）２重量％を0.２Ｎ
塩酸に混合溶解してキトサン溶液とし、１００℃で１２
０分間加熱処理して加水分解を行った。次いで、水酸化
ナトリウムを用いて pH 5.０に調整し、キトサン分解物
(B) を得た。Example 2 (1) Preparation of chitosan degradation product 2% by weight of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 0.2N.
Mix and dissolve in hydrochloric acid to make a chitosan solution,
Hydrolysis was performed by heat treatment for 0 minutes. Then adjust the pH to 5.0 with sodium hydroxide and decompose the chitosan.
I got (B).

【００３２】（２）酢酸含有液の調製 酢酸濃度１０重量％の醸造酢１リットルに対して、卵殻
３０ｇ，重炭酸ソーダ１4.０ｇ及び苛性ソーダ6.６７ｇ
を加えて混合した。このものの酢酸濃度は５重量％であ
る。(2) Preparation of acetic acid-containing liquid 30 g of eggshell, 14.0 g of sodium bicarbonate and 6.67 g of caustic soda were added to 1 liter of brewed vinegar having an acetic acid concentration of 10% by weight.
Was added and mixed. The acetic acid concentration of this product is 5% by weight.

【００３３】（３）ブラウン・ジュースの調製 刈り取ったアルファルファを搾汁機にて搾汁してグリー
ン・ジュースを得、これに１３０℃の温度の蒸気を送入
してタンパク質を凝固せしめた後、遠心分離を行って、
目的とするブラウン・ジュースを得た。(3) Preparation of Brown Juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at a temperature of 130 ° C. is fed into the juice to coagulate the protein. Centrifuge,
I got the target brown juice.

【００３４】（４）本発明の水稲有害生物防除剤の調製 前記（１）で得られたキトサン分解物(B) ４重量部、酢
酸濃度１０重量％の醸造酢0.５重量部及び純水１重量部
を混合し、水稲有害生物防除剤(C) を得た。また、前記
（１）で得られたキトサン分解物(B) ４重量部、前記
（２）で得られた酢酸含有液１重量部及び純水0.５重量
部を混合し、水稲有害生物防除剤(D)を得た。さらに、
前記（１）で得られたキトサン分解物(B) ４重量部、前
記（２）で得られた酢酸含有液１重量部及び前記（３）
で得られたブラウン・ジュース0.５重量部を混合し、水
稲有害生物防除剤(E) を得た。(4) Preparation of paddy rice pest control agent of the present invention 4 parts by weight of the chitosan degradation product (B) obtained in the above (1), 0.5 parts by weight of vinegar having an acetic acid concentration of 10% by weight and pure water 1 part by weight was mixed to obtain a rice pest control agent (C). Further, 4 parts by weight of the decomposed product of chitosan (B) obtained in (1) above, 1 part by weight of the acetic acid-containing solution obtained in (2) above and 0.5 part by weight of pure water were mixed to control pests of paddy rice. The agent (D) was obtained. further,
4 parts by weight of the decomposed product of chitosan (B) obtained in (1), 1 part by weight of the acetic acid-containing solution obtained in (2), and (3)
The brown juice 0.5 part by weight obtained in the above was mixed to obtain a paddy rice pest control agent (E).

【００３５】（５）病害防除および薬害試験 ・対象病害：イネいもち病（穂いもち） ・供試品種：コシヒカリ ・栽培様式：播種＝平成４年４月１５日、田植え＝同年
５月１５日（機械移植）、栽培密度＝１５×３０ｃｍ ・試験区：１区２０ｍ² 、３連制 ・薬剤散布方法：１０倍、２０倍、３０倍希釈の前記
（４）で得られた水稲有害生物防除剤(C),(D),(E) それ
ぞれを、出穂前の平成４年８月１０日と出穂後の同年８
月１８日の計２回、背負式全自動噴霧器を用いて、１０
ａ当り１５０リットルの割合で散布した。 ・調査方法：平成４年９月１〜３日にかけて、各区２０
株の全ての穂の発病を程度別に調査し、発病穂率及び発
病度を算出した。薬害は適宜肉眼で観察した。 ・試験結果：第５表に示した通り、本発明の水稲有害生
物防除剤(C),(D),(E)の１０倍、２０倍、３０倍希釈液
は、イネいもち病の発生を防除した。なお、水稲有害生
物防除剤(C) の１０倍希釈液散布区においては葉先が黄
化する薬害が見られた。しかし、酢酸含有液をキトサン
分解物と混合した水稲有害生物防除剤(D) 、さらに純水
の代わりにブラウンジュースを混合した水稲有害生物防
除剤(E)の１０倍希釈液散布区においては、薬害は回避
され、イネの生育は良好であった。(5) Disease control and phytotoxicity test-Target disease: Rice blast disease (ear blast) -Test variety: Koshihikari-Cultivation style: Seeding = April 15, 1992, Rice planting = May 15, the same year ( (Machine transplantation), cultivation density = 15 × 30 cm ・ Test area: 1 area 20 m ² , triple control ・ Drug spraying method: 10 times, 20 times, 30 times diluted paddy rice pest control agent obtained in the above (4) (C), (D), and (E) are each recorded on August 10, 1992 before heading and on the same year 8 after heading.
18 times a month, 10 times using a backpack type fully automatic sprayer
It was sprayed at a rate of 150 liters per a.・ Survey method: From September 1 to 3, 1992, each ward 20
The disease occurrence of all panicles of the strain was investigated by degree, and the disease occurrence rate and the disease severity were calculated. The phytotoxicity was appropriately observed with the naked eye. -Test results: As shown in Table 5, the 10-fold, 20-fold, and 30-fold dilutions of the paddy rice pest control agents (C), (D), and (E) of the present invention caused the occurrence of rice blast. Controlled. In addition, in the 10-fold diluted solution sprayed area of the paddy rice pest control agent (C), phytotoxicity of leaf tips was observed. However, in the paddy rice pest control agent (D) in which an acetic acid-containing solution was mixed with a decomposed product of chitosan, and in the paddy rice pest control agent (E) in which brown juice was mixed in place of pure water, a 10-fold diluted solution was applied, The phytotoxicity was avoided and the growth of rice was good.

【００３６】[0036]

【表５】 [Table 5]

【００３７】〔第５表の脚注〕 ＊１：発病穂率＝首いもち率＋枝梗いもち率 ＊２：発病の程度 I ＝２／３以上の枝梗いもちの発病が認められる。 II＝１／３以上２／３未満の枝梗いもちの発病が認めら
れる。 III ＝１／３未満の枝梗いもちの発病が認められる。[Footnote in Table 5] * 1: Occurrence rate of diseased ear = rate of neck blast + rate of branch blast * 2: Degree of disease I = 2/3 or more occurrence of branch blast is recognized. II = 1/3 or more and less than 2/3, the occurrence of branch blast blast is recognized. III = less than 1/3 of the incidence of branch blast is recognized.

【００３８】[0038]

【数１】 [Equation 1]

【００３９】[0039]

【数２】 [Equation 2]

【００４０】実施例３ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）２重量％を３％(
W/V ) の酢酸溶液に溶解してキトサン溶液とし、１２１
℃で６０分間加圧加熱処理して、加水分解を行った。次
いで、水酸化ナトリウムを用いて、pH 5. ０に調整し、
キトサン分解物(A) を得た。Example 3 (1) Preparation of Decomposed Product of Chitosan 2% by weight of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 3% (
W / V) acetic acid solution to make chitosan solution, 121
Hydrolysis was performed by heating under pressure at 60 ° C. for 60 minutes. Then adjust the pH to 5.0 with sodium hydroxide,
A decomposed product of chitosan (A) was obtained.

【００４１】（２）酢酸含有液の調製 酢酸濃度１０重量％の醸造酢１リットルに対して、卵殻
３０ｇ，重炭酸ソーダ１4.０ｇ及び苛性ソーダ6.６７ｇ
を加えて混合した。この酢酸含有液の酢酸濃度は５重量
％である。(2) Preparation of acetic acid-containing liquid 30 g of eggshell, 14.0 g of sodium bicarbonate and 6.67 g of caustic soda were added to 1 liter of brewed vinegar having an acetic acid concentration of 10% by weight.
Was added and mixed. The acetic acid concentration of this acetic acid-containing liquid is 5% by weight.

【００４２】（３）ブラウン・ジュースの調製 刈り取ったアルファルファを搾汁機にて搾汁してグリー
ン・ジュースを得、これに１３０℃の温度の蒸気を送入
してタンパク質を凝固せしめた後、遠心分離を行って、
目的とするブラウン・ジュースを得た。(3) Preparation of Brown Juice The cut alfalfa is squeezed by a squeezing machine to obtain green juice, and steam at a temperature of 130 ° C. is fed into the juice to coagulate the protein. Centrifuge,
I got the target brown juice.

【００４３】（４）本発明の水稲有害生物防除剤の調製 前記（１）で得られたキトサン分解物(A) ４重量部、酢
酸濃度１０重量％の醸造酢0.５重量部及び純水１重量部
を混合し、水稲有害生物防除剤(F) を得た。また、前記
（１）で得られたキトサン分解物(A) ４重量部、前記
（２）で得られた酢酸含有液１重量部及び純水0.５重量
部を混合し、水稲有害生物防除剤(G)を得た。さらに、
前記（１）で得られたキトサン分解物(A) ４重量部、前
記（２）で得られた酢酸含有液１重量部及び前記（３）
で得られたブラウン・ジュース0.５重量部を混合し、植
物有害生物防除剤(H) を得た。(4) Preparation of paddy rice pest control agent of the present invention 4 parts by weight of the chitosan degradation product (A) obtained in the above (1), 0.5 parts by weight of brewed vinegar having an acetic acid concentration of 10% by weight and pure water. 1 part by weight was mixed to obtain a rice pest control agent (F). Further, 4 parts by weight of the decomposed product of chitosan (A) obtained in (1) above, 1 part by weight of the acetic acid-containing solution obtained in (2) above and 0.5 part by weight of pure water were mixed to control pests of paddy rice. The agent (G) was obtained. further,
4 parts by weight of the chitosan degradation product (A) obtained in (1), 1 part by weight of the acetic acid-containing solution obtained in (2), and (3)
0.5 parts by weight of the brown juice obtained in step 1 were mixed to obtain a plant pest control agent (H).

【００４４】（５）病害防除および薬害試験 ・対象病害：イネ紋枯病 ・供試品種：コシヒカリ ・栽培様式：播種＝平成４年３月４日、田植え＝同年４
月２日（機械移植）、栽培密度＝１５×３０ｃｍ ・試験区：１区２０ｍ² 、３連制 ・薬剤散布方法：１０倍、２０倍、３０倍希釈の前記
（４）で得られた水稲有害生物防除剤(F),(G),(H) それ
ぞれを、出穂前の平成４年６月２６日と出穂後の同年７
月５日の計２回、背負式全自動噴霧器を用いて、１０ａ
当り１５０リットルの割合で散布した。 ・調査方法：平成４年７月２５日に、各区２０株につい
て、草丈及び最上位病斑高を計測し、病斑高率、発病株
の被害度、全体の被害度を算出した。薬害は適宜肉眼で
観察した。 ・試験結果：第６表に示した通り、本発明の水稲有害生
物防除剤(F),(G),(H)の１０倍、２０倍、３０倍希釈液
は、イネ紋枯病の発生を防除した。なお、水稲有害生物
防除剤(F) の１０倍希釈液散布区においては葉先が黄化
する薬害が見られた。しかし、酢酸含有液をキトサン分
解物と混合した水稲有害生物防除剤(G)、さらに純水の
代わりにブラウンジュースを混合した水稲有害生物防除
剤(H) の１０倍希釈液散布区においては、薬害は回避さ
れ、イネの生育は良好であった。(5) Disease control and phytotoxicity test-Target disease: Rice blight-Test variety: Koshihikari-Cultivation style: Seeding = March 4, 1992, Rice planting = 4 of the same year
2nd (machine transplantation), cultivation density = 15 × 30cm ・ Test area: 1 area 20m ² , triple control ・ Drug spraying method: 10 times, 20 times, 30 times diluted paddy rice obtained in (4) above The pest control agents (F), (G), and (H) were treated on June 26, 1992 before heading and on the same year 7 after heading respectively.
5 times a month, 10a using a backpack type fully automatic sprayer
It was sprayed at a rate of 150 liters per one. -Investigation method: On July 25, 1992, the plant height and the highest lesion height were measured for each of the 20 strains in each ward, and the lesion height ratio, the severity of the diseased strain, and the overall damage were calculated. The phytotoxicity was appropriately observed with the naked eye. -Test results: As shown in Table 6, the 10-fold, 20-fold, and 30-fold dilutions of the paddy rice pest control agents (F), (G), and (H) of the present invention show the occurrence of rice blight. Control. In addition, in the 10-fold diluted solution sprayed with paddy rice pest control agent (F), phytotoxicity of leaf tips was observed. However, in the paddy rice pest control agent (G) in which an acetic acid-containing liquid was mixed with a decomposed product of chitosan, and in the paddy rice pest control agent (H) in which brown juice was mixed in place of pure water, a 10-fold diluted solution was applied. The phytotoxicity was avoided and the rice growth was good.

【００４５】[0045]

【表６】 [Table 6]

【００４６】〔第６表の脚注〕[Footnote in Table 6]

【００４７】[0047]

【数３】 [Equation 3]

【００４８】[0048]

【数４】 [Equation 4]

【００４９】[0049]

【数５】 [Equation 5]

【００５０】[0050]

【数６】 [Equation 6]

【００５１】[0051]

【数７】 [Equation 7]

【００５２】実施例４ （１）キトサン分解物の調製 キトサン（東京化成工業株式会社製）5.０ｇを、市販食
酢〔酢酸濃度4.２％(W/V ) 〕１００ｍｌに溶解した。
次いで、酵素製剤スミチームＡＰ２（新日本化学工業
（株）製）0.１ｇを加え、５０℃恒温水槽中でゆっくり
攪拌しながら、６時間反応せしめた。反応終了後、沸騰
水中に５分間保ち、酵素を失活せしめた。しかる後、水
酸化ナトリウムを用いて、pH 5. ０に調製し、キトサン
分解物(D)を得た。Example 4 (1) Preparation of Decomposed Product of Chitosan 5.0 g of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was dissolved in 100 ml of commercial vinegar [acetic acid concentration 4.2% (W / V)].
Next, 0.1 g of the enzyme preparation Sumiteam AP2 (manufactured by Shin Nippon Chemical Industry Co., Ltd.) was added, and the mixture was reacted for 6 hours while slowly stirring in a constant temperature water bath at 50 ° C. After completion of the reaction, the enzyme was inactivated by keeping it in boiling water for 5 minutes. Then, the pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan degradation product (D).

【００５３】（２）酢酸含有液の調製 酢酸濃度１０重量％の醸造酢１リットルに対して、卵殻
３０ｇ，重炭酸ソーダ１4.０ｇ及び苛性ソーダ6.６７ｇ
を加えて混合した。この酢酸含有液の酢酸濃度は５重量
％である。(2) Preparation of acetic acid-containing liquid 30 g of eggshell, 14.0 g of sodium bicarbonate and 6.67 g of caustic soda were added to 1 liter of brewed vinegar having an acetic acid concentration of 10% by weight.
Was added and mixed. The acetic acid concentration of this acetic acid-containing liquid is 5% by weight.

【００５４】（３）ブラウン・ジュースの調製 刈り取ったアルファルファを搾汁機にて搾汁してグリー
ン・ジュースを得、これに１３０℃の温度の蒸気を送入
してタンパク質を凝固せしめた後、遠心分離を行って、
目的とするブラウン・ジュースを得た。(3) Preparation of Brown Juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at a temperature of 130 ° C. is fed into the juice to coagulate the protein. Centrifuge,
I got the target brown juice.

【００５５】（４）本発明の水稲有害生物防除剤の調製 前記（１）で得られたキトサン分解物(D) ２重量部、酢
酸濃度１０重量％の醸造酢１重量部及び純水２重量部を
混合し、水稲有害生物防除剤(I) を得た。次に、前記
（１）で得られたキトサン分解物(D) ２重量部、前記
（２）で得られた酢酸含有液２重量部及び純水１重量部
を混合し、水稲有害生物防除剤(J) を得た。また、前記
（１）で得られたキトサン分解物(D) ２重量部、酢酸濃
度１０重量％の醸造酢１重量部、純水１重量部及び前記
（３）で得られたブラウン・ジュース１重量部を混合
し、水稲有害生物防除剤(K) を得た。さらに、前記
（１）で得られたキトサン分解物(D) ２重量部、前記
（２）で得られた酢酸含有液２重量部及び前記（３）で
得られたブラウン・ジュース１重量部を混合し、水稲有
害生物防除剤(L) を得た。(4) Preparation of Paddy Rice Pest Control Agent of the Present Invention 2 parts by weight of the chitosan degradation product (D) obtained in the above (1), 1 part by weight of vinegar having an acetic acid concentration of 10% by weight and 2 parts by weight of pure water. The parts were mixed to obtain a paddy rice pest control agent (I). Next, 2 parts by weight of the decomposed product of chitosan (D) obtained in (1) above, 2 parts by weight of the acetic acid-containing solution obtained in (2) above and 1 part by weight of pure water were mixed to obtain a pest control agent for paddy rice. I got (J). Further, 2 parts by weight of the decomposed product of chitosan (D) obtained in the above (1), 1 part by weight of brewed vinegar having an acetic acid concentration of 10% by weight, 1 part by weight of pure water, and brown juice 1 obtained in the above (3) The parts by weight were mixed to obtain a rice pest control agent (K). Furthermore, 2 parts by weight of the chitosan decomposition product (D) obtained in the above (1), 2 parts by weight of the acetic acid-containing solution obtained in the above (2) and 1 part by weight of the brown juice obtained in the above (3) were added. The mixture was mixed to obtain a paddy rice pest control agent (L).

【００５６】（５）病害防除および薬害試験 ・対象病害：イネ内穎褐変病 ・供試品種：ヤマヒカリ ・栽培様式：播種＝平成３年５月１２日、田植え＝同年
６月５日（機械移植）、栽培密度＝１５×３０ｃｍ ・試験区：１区３０ｍ² 、３連制 ・薬剤散布方法：２０倍、５０倍、１００倍希釈の前記
（４）で得られた水稲有害生物防除剤(I),(J),(K),(L)
それぞれを、出穂前の平成３年８月２３日に、背負式全
自動噴霧器を用いて、１０ａ当り１５０リットルの割合
で散布した。 ・調査方法：平成３年９月１５日に、各区４０株を任意
に抽出し、１株５穂について、発病程度別に調査し、発
病穂率及び発病度を算出した。薬害は適宜肉眼で観察し
た。 ・試験結果：第７表に示した通り、本発明の水稲有害生
物防除剤(I),(J),(K),(L) の２０倍、５０倍、１００倍
希釈液は、イネ内穎褐変病の発生を防除した。なお、水
稲有害生物防除剤(I) の２０倍希釈液散布区においては
葉先が著しく黄化する薬害が見られた。しかし、酢酸含
有液を用いた水稲有害生物防除剤(J) の２０倍希釈液散
布区においては薬害は軽減され、さらに純水の代わりに
ブラウンジュースを混合した水稲有害生物防除剤(K) の
２０倍希釈液散布区と、酢酸含有液を用い、かつ、純水
の代わりにブラウンジュースを混合した水稲有害生物防
除剤(L) の２０倍希釈液散布区においては、薬害は完全
に回避され、イネの生育は良好であった。(5) Disease control and phytotoxicity test-Target disease: Brown sprouts in rice-Test variety: Yamahikari-Cultivation style: Seeding = May 12, 1991, Rice planting = June 5, 1959 (machine transplantation) ), Cultivation density = 15 × 30 cm ・ Test area: 1 area 30 m ² , 3 consecutive times ・ Drug spraying method: 20 times, 50 times, 100 times diluted paddy rice pest control agent (I) obtained in (4) above ), (J), (K), (L)
On August 23, 1991, before heading, each was sprayed at a rate of 150 liters per 10a using a backpack type automatic sprayer. -Investigation method: On September 15, 1991, 40 strains in each ward were arbitrarily extracted, and 5 ears per strain were investigated according to the degree of illness, and the diseased ears rate and the degree of illness were calculated. The phytotoxicity was appropriately observed with the naked eye. -Test results: As shown in Table 7, the rice pest control agents (I), (J), (K), and (L) of the present invention were diluted 20-fold, 50-fold, and 100-fold in rice. The occurrence of spear browning disease was controlled. In addition, in the 20-fold diluted solution sprayed with paddy rice pest control agent (I), phytotoxicity was observed in which the leaf tips were significantly yellowed. However, the phytotoxicity was reduced in the 20-fold diluted solution spraying of paddy rice pest control agent (J) using acetic acid-containing liquid, and the paddy rice pest control agent (K) containing brown juice instead of pure water was added. Chemical damage was completely avoided in the 20-fold diluted liquid spraying group and the 20-fold diluted liquid sprayed group of the paddy rice pest control agent (L) in which acetic acid-containing liquid was used and brown juice was mixed in place of pure water. The growth of rice was good.

【００５７】[0057]

【表７】 [Table 7]

【００５８】〔第７表の脚注〕[Footnote in Table 7]

【００５９】[0059]

【数８】 [Equation 8]

【００６０】[0060]

【数９】 [Equation 9]

【００６１】[0061]

【発明の効果】本発明の防除剤によれば、イネいもち
病，イネ紋枯病，イネ内穎褐変病などの原因となる水稲
の有害生物を有効に防除することができる。しかも、本
発明の防除剤は、低濃度の使用で有効であり、薬害も少
ない。EFFECTS OF THE INVENTION According to the control agent of the present invention, it is possible to effectively control pests of paddy rice which cause rice blast, rice blight, rice inner brown brown disease and the like. Moreover, the pesticide of the present invention is effective even when used at a low concentration and has little chemical damage.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.⁵ 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ａ０１Ｎ 63:00） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Office reference number FI technical display area A01N 63:00)

Claims (5)

【特許請求の範囲】[Claims] 【請求項１】 キトサン分解物１重量部あたり、酢酸を
0.２５〜４重量部の割合で配合してなる水稲のいもち病
防除剤。1. Acetic acid is added to 1 part by weight of a decomposed product of chitosan.
A blast control agent for paddy rice, which is formulated in a proportion of 0.25 to 4 parts by weight. 【請求項２】 キトサン分解物１重量部あたり、酢酸を
0.２５〜４重量部の割合で配合してなる水稲の紋枯病防
除剤。2. Acetic acid is added to 1 part by weight of a decomposed product of chitosan.
An agent for controlling rice blight of paddy rice, which is compounded in a ratio of 0.25 to 4 parts by weight. 【請求項３】 キトサン分解物１重量部あたり、酢酸を
0.２５〜４重量部の割合で配合してなる水稲の内穎褐変
病防除剤。3. Acetic acid is added to 1 part by weight of a decomposed product of chitosan.
An inner brown brown disease control agent for paddy rice, which is compounded in a proportion of 0.25 to 4 parts by weight. 【請求項４】 第３成分としてアルファルファ緑葉脱蛋
白質上澄液を配合してなる請求項１〜３のいずれかに記
載の水稲の有害生物防除剤。4. The pest control agent for paddy rice according to claim 1, which comprises an alfalfa green leaf deproteinization supernatant as a third component. 【請求項５】 請求項１〜４のいずれかに記載の水稲の
有害生物防除剤を用いた水稲有害生物の防除方法。5. A method for controlling paddy rice pests using the paddy rice pest control agent according to any one of claims 1 to 4.