JPH06226273A - Ozone sterilization method for aqueous solution system - Google Patents
Ozone sterilization method for aqueous solution systemInfo
- Publication number
- JPH06226273A JPH06226273A JP1640593A JP1640593A JPH06226273A JP H06226273 A JPH06226273 A JP H06226273A JP 1640593 A JP1640593 A JP 1640593A JP 1640593 A JP1640593 A JP 1640593A JP H06226273 A JPH06226273 A JP H06226273A
- Authority
- JP
- Japan
- Prior art keywords
- ozone
- bacteria
- aqueous solution
- plankton
- sterilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、培養設備の培地（海
水、淡水）、養魚設備、活魚輸送設備、浄水設備等に適
用されるバクテリアと有用微生物を含む水溶液系の殺菌
方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for sterilizing an aqueous solution containing bacteria and useful microorganisms, which is applied to culture media (seawater, fresh water), fish farming equipment, live fish transporting equipment, water purification equipment and the like.
【０００２】[0002]
【従来の技術】プランクトンを純粋培養中、培養初期に
培地やプランクトン表面に付着していたバクテリア、又
は培養中エアーバブリングにより外界から侵入したバク
テリアが異常繁殖し目的のプランクトンの増殖に悪影響
を及ぼすことがある。2. Description of the Related Art During the pure culture of plankton, bacteria attached to the medium or the surface of plankton at the initial stage of culture, or bacteria invading from the outside due to air bubbling during culture abnormally propagate and adversely affect the growth of the target plankton. There is.
【０００３】この対策のため、プランクトンネットを用
いてプランクトンを主体に採取するか、更に純度をあげ
るにはピペット等ですくいあげる方法がとられている。As a countermeasure against this, a plankton is mainly collected using a plankton net, or a pipette or the like is used to further improve the purity.
【０００４】[0004]
【発明が解決しようとする課題】プランクトンとバクテ
リアが混在する培地中からプランクトンネットやピペッ
トにより機械的作業によりプランクトンを抽出する前記
の従来の方法では、プランクトンに付着しているバクテ
リアや培地中に存在するバクテリアも一部プランクトン
に付随してくるため、バクテリアの混入を完全に防止す
ることができず、また、手作業によるため大量処理に向
かない等の問題点がある。DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention In the above-mentioned conventional method of extracting plankton by a mechanical operation with a plankton net or pipette from a medium in which plankton and bacteria are mixed, the presence in the bacteria or medium adhering to plankton Since some bacteria also accompany plankton, it is not possible to completely prevent the contamination of bacteria, and it is not suitable for large-scale processing because it is done manually.
【０００５】本発明は、以上の問題点を解決することが
できる水溶液系のオゾン殺菌方法を提供しようとするも
のである。The present invention is intended to provide an aqueous solution type ozone sterilization method capable of solving the above problems.
【０００６】[0006]
【課題を解決するための手段】本発明の水溶液系のオゾ
ン殺菌方法は、バクテリア及び有用微生物を含む水溶液
系において、オゾンを０．２〜１．５ｐｐｍ注入してオ
ゾンによる殺菌を１０秒以上行い、その後水溶液中の残
留オキシダントを０．２ｐｐｍ以下に除去し、水溶液中
のバクテリアのみを選択的に殺菌することを特徴とす
る。The ozone sterilization method of an aqueous solution system of the present invention is to sterilize by ozone for 10 seconds or more by injecting 0.2 to 1.5 ppm of ozone in an aqueous solution system containing bacteria and useful microorganisms. After that, residual oxidant in the aqueous solution is removed to 0.2 ppm or less, and only bacteria in the aqueous solution are selectively sterilized.
【０００７】[0007]
【作用】プランクトン又は酵母等の有効微生物とバクテ
リアが混在する水溶液にオゾンを注入するとプランクト
ン，バクテリアは、図１に示すように、オゾン耐性の違
いによりオゾン濃度と曝露時間により両者の生存率に著
しい差が生じる。即ち、バクテリアとしてのビブリオ菌
はオゾン濃度０．５ｐｐｍで殺菌時間０．５分（３０
秒）とれば完全に殺菌できる。一方プランクトンについ
ては、例えばクロレラの場合オゾン濃度１．０ｐｐｍ殺
菌時間１０分でも生存率は１００％である。また、ドナ
リエラの場合、オゾン濃度１．０ｐｐｍでも殺菌時間
０．５分（３０秒）であればその生存率は全く問題な
い。しかし、殺菌時間０．５分でオゾン濃度が２．０ｐ
ｐｍになると、図３に示すように、ドナリエラは損傷が
大きく培養中に死滅していく。また、図３に示すよう
に、オゾン濃度１．５ｐｐｍで殺菌時間が０．５分の場
合には、ドナリエラのプランクトン濃度はほぼ一定でド
ナリエラは殆ど増殖しなくなる。オゾン濃度０．５ｐｐ
ｍでも殺菌時間が１分以上になると、図１に示すように
ドナリエラの生存率は下がる。[Function] When ozone is injected into an aqueous solution in which effective microorganisms such as plankton or yeast and bacteria are mixed, plankton and bacteria show remarkable survival rates depending on ozone concentration and exposure time, as shown in FIG. There is a difference. That is, Vibrio bacteria as bacteria have an ozone concentration of 0.5 ppm and a sterilization time of 0.5 minutes (30 minutes).
You can completely sterilize if you take seconds). On the other hand, for plankton, for example, in the case of Chlorella, the survival rate is 100% even if the ozone concentration is 1.0 ppm and the sterilization time is 10 minutes. In the case of Donariella, even if the ozone concentration is 1.0 ppm, if the sterilization time is 0.5 minutes (30 seconds), there is no problem in the survival rate. However, when the sterilization time is 0.5 minutes, the ozone concentration is 2.0p.
At pm, as shown in FIG. 3, Donariella is heavily damaged and dies during culture. Further, as shown in FIG. 3, when the ozone concentration is 1.5 ppm and the sterilization time is 0.5 minutes, the plankton concentration of Donariella is almost constant and the Donariella hardly grows. Ozone concentration 0.5pp
Even in m, if the sterilization time is 1 minute or more, the survival rate of Donariella decreases as shown in FIG.
【０００８】一方、図１に示すように、オゾンによるバ
クテリアの殺菌性はオゾン濃度の増加によって増加する
が、オゾン濃度０．２ｐｐｍ以上の場合にバクテリアの
殺菌効果が認められる。On the other hand, as shown in FIG. 1, the bactericidal property of bacteria by ozone increases with an increase in ozone concentration, but a bactericidal effect of bacteria is recognized when the ozone concentration is 0.2 ppm or more.
【０００９】更に、図４に示すように、オゾン濃度１．
５ｐｐｍでは殺菌時間１０秒でビブリオ菌の生菌率は１
０-7以下（殺菌率９９．９９９９９％以上）となり、オ
ゾン濃度０．５ｐｐｍでは殺菌時間３０秒で生菌率１０
-8以下（殺菌率９９．９９９９９９％以上）となり、ほ
ぼ完全な殺菌が行われる。Further, as shown in FIG.
At 5 ppm, the viability of Vibrio is 1 in 10 seconds for sterilization.
0 -7 or less (kill rate 99.99999 higher), and the viable cell ratio in the ozone concentration 0.5ppm sterilizing time 30 sec 10
-8 or less (sterilization rate 99.999999% or more), almost complete sterilization is performed.
【００１０】このようにオゾン注入量と殺菌時間（曝露
時間）を対象微生物によって適切な値に設定することに
よりバクテリアのみを選択的に殺菌することが可能であ
る。By thus setting the ozone injection amount and the sterilization time (exposure time) to appropriate values depending on the target microorganism, it is possible to selectively sterilize only bacteria.
【００１１】本発明では、以上のオゾンによるバクテリ
アの殺菌性を考慮して水溶液系に注入するオゾンの濃度
の下限を０．２ｐｐｍとし、かつ、前述したように、プ
ランクトン等の有効微生物の生存率を考慮して水溶液系
に注入するオゾン濃度の上限を１．５ｐｐｍとした。ま
た、図４に示すバクテリア（ビブリオ菌）の生菌率に基
づき、オゾンによる殺菌時間は１０秒以上とした。In the present invention, the lower limit of the concentration of ozone injected into the aqueous solution system is set to 0.2 ppm in consideration of the bactericidal property of bacteria by ozone as described above, and the survival rate of effective microorganisms such as plankton is set as described above. Taking the above into consideration, the upper limit of the ozone concentration injected into the aqueous solution system was set to 1.5 ppm. Further, the sterilization time with ozone was set to 10 seconds or more based on the viable cell ratio of the bacteria (Vibrio bacterium) shown in FIG.
【００１２】以上のように、本発明では、オゾンを水溶
液系に０．２〜１．５ｐｐｍ注入して１０秒以上オゾン
による殺菌を行うことによって、プランクトン、酵母等
の有用微生物を生存させバクテリアのみが選択的に殺菌
される。As described above, according to the present invention, by injecting 0.2 to 1.5 ppm of ozone into an aqueous solution system and sterilizing with ozone for 10 seconds or more, useful microorganisms such as plankton and yeast are allowed to survive and only bacteria are allowed to survive. Are selectively sterilized.
【００１３】また、バクテリア殺菌後の残留オキシダン
トは、微量であっても或る時間曝露されれば、プランク
トン、酵母等の有用微生物の増殖に悪影響を及ぼす。Further, the residual oxidant after sterilization of bacteria has a bad influence on the growth of useful microorganisms such as plankton and yeast when exposed for a certain amount of time even if the amount thereof is very small.
【００１４】本発明では、前記のオゾンによるバクテリ
アの殺菌後残留オキシダントを０．２ｐｐｍ以下に除去
することによって、有用微生物の増殖に支障がないよう
にする。In the present invention, the residual oxidant after sterilization of bacteria by ozone is removed to 0.2 ppm or less so that the growth of useful microorganisms is not hindered.
【００１５】また、本発明におけるオゾンによる殺菌は
溶菌作用によって行われるために、塩素殺菌におけるよ
うに、有用微生物に因伝子障害を生じさせることがな
い。Further, since the sterilization by ozone in the present invention is carried out by the bacteriolytic action, it does not cause the gene damage to useful microorganisms as in the chlorine sterilization.
【００１６】なお、本発明における残留オキシダントの
除去方法としては、エアーバブリング、活性炭注入、電
解還元、チオ硫酸ソーダ又は亜硫酸カルシウムの滴下等
を用いることができる。エアーバブリングは培地中のオ
キシダントを曝気による気相中に放散させることがで
き、活性炭注入は培地中のオキシダントを吸着除去しす
ることができ、電解還元はカソード電極面上でオキシダ
ントを還元することができ、チオ硫酸ソーダ又は亜硫酸
カルシウムの滴下は化学反応によってオキシダントを還
元することができる。As a method for removing the residual oxidant in the present invention, air bubbling, activated carbon injection, electrolytic reduction, dropping of sodium thiosulfate or calcium sulfite, etc. can be used. Air bubbling can diffuse the oxidant in the medium into the gas phase by aeration, activated carbon injection can adsorb and remove the oxidant in the medium, and electrolytic reduction can reduce the oxidant on the cathode electrode surface. Yes, the addition of sodium thiosulfate or calcium sulfite can reduce the oxidant by a chemical reaction.
【００１７】また、オゾン濃度１．５ｐｐｍにおいて殺
菌時間が３０秒になると、図３に示すように、プランク
トン（ドナリエラ）が殆ど増殖しなくなるので、オゾン
に対して耐性があるプランクトン、例えばクロレラを用
いる場合以外には、オゾンによる殺菌時間を３０秒以下
とすることが望ましい。When the sterilization time reaches 30 seconds at an ozone concentration of 1.5 ppm, plankton (Donariella) hardly grows as shown in FIG. 3, so a plankton resistant to ozone, for example, chlorella is used. In other cases, it is desirable to set the ozone sterilization time to 30 seconds or less.
【００１８】[0018]
【実施例】ビブリオ菌１０5 〜１０6 個／ｍｌ、クロレ
ラ１０4 〜１０5 ｃｅｌｌ／ｍｌを含む海水に、オゾン
濃度１．０ｐｐｍとなるよう高濃度オゾン水を注入し攪
拌し、１分及び５分この状態を維持してオゾンによるバ
クテリアの殺菌を行った。Example High-concentration ozone water was injected into seawater containing 10 5 to 10 6 cells / ml of Vibrio bacterium and 10 4 to 10 5 cells / ml of chlorella, and the mixture was stirred for 1 minute. The bacteria were sterilized by ozone while maintaining this state for 5 minutes.
【００１９】その上で、海水中の残留オキシダント濃度
が０．２ｐｐｍ以下になるまでエアーバブリングをし、
バクテリア殺菌を停止させた。なお、殺菌時間１分のサ
ンプルについては、標準寒天培地で培養し、バクテリア
が生存していないことを確認した。Then, air bubbling is performed until the residual oxidant concentration in seawater becomes 0.2 ppm or less,
Bacterial sterilization was stopped. The sample having a sterilization time of 1 minute was cultured on a standard agar medium to confirm that the bacteria did not survive.
【００２０】その後クロレラを含む殺菌海水に栄養塩
（ＫＮＯ3 ７２ｍｇ／ｌ ＫＨ2 ＰＯ4 ４．５ｍｇ
／ｌ）を添加し１５０００Ｌｕｘの白色蛍光燈を照射し
ながら室温で２週間培養し、その増殖特性を調べた。こ
の結果を図２に示す。オゾン注入１分及び５分後に残留
オキシダントを除去したものは、オゾン注入しないもの
や、オゾン注入しても残留オキシダントを除去しないも
のに比べ、クロレラの増殖速度が著しく早い（例えば５
日間で約２０〜３０倍早い）ことが分った。Thereafter, nutrient salts (KNO 3 72 mg / l KH 2 PO 4 4.5 mg were added to sterilized seawater containing chlorella.
/ L) was added, and the cells were cultured at room temperature for 2 weeks while irradiating with a 15000 Lux white fluorescent lamp, and their growth characteristics were examined. The result is shown in FIG. Chlorella has a significantly higher growth rate (eg, 5% or less) in the case of removing residual oxidant after 1 and 5 minutes of ozone injection, as compared with the case of not injecting ozone or the method of removing residual oxidant even after ozone injection.
About 20-30 times faster in a day).
【００２１】[0021]
【発明の効果】本発明は、特許請求の範囲に記載された
構成を具備することによって、次の効果を奏することが
できる。 （１）病原性細菌等のバクテリアと有用微生物の混合水
溶液系において、バクテリアのみを低コストで完全に殺
菌することができる。 （２）無殺菌や残留オキシダント除去しないものに比べ
本発明によるプランクトン増殖速度は５日間で約２０〜
３０倍と著しく早い。 （３）オゾンは溶菌作用を有するために、塩素殺菌では
有用微生物に因伝子障害を生じさせるが本発明ではこれ
を生ずることがない。EFFECTS OF THE INVENTION The present invention has the following effects by having the structure described in the claims. (1) In a mixed aqueous solution system of bacteria such as pathogenic bacteria and useful microorganisms, only bacteria can be completely sterilized at low cost. (2) The plankton growth rate according to the present invention is about 20 to 5 in 5 days as compared with those without sterilization or without removal of residual oxidant.
Remarkably fast at 30 times. (3) Since ozone has a bacteriolytic action, chlorine sterilization causes gene damage to useful microorganisms, but this does not occur in the present invention.
【図１】オゾンの殺菌効果を示す生菌率とオゾン濃度と
の関係のグラフである。FIG. 1 is a graph showing the relationship between the viable cell ratio showing the bactericidal effect of ozone and the ozone concentration.
【図２】本発明の一実施例における残留オキシダントの
プランクトン増殖への影響を示すバクテリア濃度と培養
時間の関係グラフである。FIG. 2 is a graph showing the relationship between bacterial concentration and culture time showing the effect of residual oxidant on plankton growth in one example of the present invention.
【図３】オゾン注入量のプランクトン（ドナリエラ）増
殖への影響を示すプランクトン濃度と培養時間の関係の
グラフである。FIG. 3 is a graph showing the relationship between plankton concentration and culture time, which shows the influence of the amount of injected ozone on the growth of plankton (Donariella).
【図４】オゾン処理時間とビブリオ菌の生菌率の関係を
示すグラフである。FIG. 4 is a graph showing the relationship between ozone treatment time and viable cell viability of Vibrio.
Claims (1)
において、オゾンを０．２〜１．５ｐｐｍ注入してオゾ
ンによる殺菌を１０秒以上行い、その上で水溶液中の残
留オキシダントを０．２ｐｐｍ以下に除去し、水溶液中
のバクテリアのみを選択的に殺菌することを特徴とする
水溶液系のオゾン殺菌方法。1. In an aqueous solution system containing bacteria and useful microorganisms, ozone is injected in an amount of 0.2 to 1.5 ppm and sterilized by ozone for 10 seconds or longer, and then the residual oxidant in the aqueous solution is reduced to 0.2 ppm or less. An aqueous solution-based ozone sterilization method, which comprises removing and selectively sterilizing only bacteria in the aqueous solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1640593A JPH06226273A (en) | 1993-02-03 | 1993-02-03 | Ozone sterilization method for aqueous solution system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1640593A JPH06226273A (en) | 1993-02-03 | 1993-02-03 | Ozone sterilization method for aqueous solution system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06226273A true JPH06226273A (en) | 1994-08-16 |
Family
ID=11915340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1640593A Withdrawn JPH06226273A (en) | 1993-02-03 | 1993-02-03 | Ozone sterilization method for aqueous solution system |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06226273A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06253819A (en) * | 1993-03-09 | 1994-09-13 | Agency Of Ind Science & Technol | Method of culturing plankton |
| US11247922B2 (en) | 2011-04-12 | 2022-02-15 | Silver Bullet Water Treatment Company, Llc | Water treatment systems and methods |
-
1993
- 1993-02-03 JP JP1640593A patent/JPH06226273A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06253819A (en) * | 1993-03-09 | 1994-09-13 | Agency Of Ind Science & Technol | Method of culturing plankton |
| US11247922B2 (en) | 2011-04-12 | 2022-02-15 | Silver Bullet Water Treatment Company, Llc | Water treatment systems and methods |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20000404 |