JPH06222060A - Flow cell - Google Patents
Flow cellInfo
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- JPH06222060A JPH06222060A JP3133093A JP3133093A JPH06222060A JP H06222060 A JPH06222060 A JP H06222060A JP 3133093 A JP3133093 A JP 3133093A JP 3133093 A JP3133093 A JP 3133093A JP H06222060 A JPH06222060 A JP H06222060A
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- carrier
- liquid
- flow
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、試料液中に含有される
分析対象物、特に核酸、抗原、抗体等の生体関連の微量
物質を、これらと選択的に結合する物質を坦持する担体
を用いて分析するフローセルに関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a carrier that carries a substance that selectively binds an analyte contained in a sample liquid, particularly a trace substance related to a living body such as nucleic acid, antigen and antibody. The present invention relates to a flow cell to be analyzed using.
【０００２】[0002]
【従来の技術】従来、生体関連の微量物質を分析するた
めには、例えば「検査と技術vol6 NO.7,1988」に開示さ
れているようなＲＩＡ（放射性免疫測定法）、ＥＩＡ
（酵素免疫測定法）、ＦＩＡ（蛍光免疫測定法）等が利
用されている。ここでは、試料液中の微量物質と選択的
に結合する試薬をフィルタやラテックス粒子、ガラスビ
ーズ等の固体材料に坦持させ、その試薬に微量物質を結
合させた結合体を放射性的、光学的、電気的に検出する
ようになっている。このような固体材料は一般に担体と
呼ばれ、微量物質を分析する速度や感度は、担体が微量
物質と結合する速さや量に左右される。従って、担体に
は反応面積つまり比表面積が大きく、反応時の運動能力
つまり撹伴が良好なラテックス粒子等が主に使用されて
いる。2. Description of the Related Art Conventionally, in order to analyze a trace substance related to a living body, for example, RIA (radioimmunoassay), EIA as disclosed in "Inspection and Technology vol6 NO.7,1988".
(Enzyme immunoassay), FIA (fluorescent immunoassay) and the like are used. Here, a reagent that selectively binds to a trace substance in a sample solution is carried on a solid material such as a filter, latex particles, or glass beads, and a conjugate obtained by binding the trace substance to the reagent is radioactively or optically. , Is designed to be detected electrically. Such a solid material is generally called a carrier, and the speed and sensitivity of analyzing a trace substance depend on the speed and amount of the carrier binding to the trace substance. Therefore, as the carrier, latex particles having a large reaction area, that is, a large specific surface area, and having good motility during reaction, that is, agitation, are mainly used.
【０００３】このような結合体を検出するためには、微
細な流通部を有するフローセルが使用される。特に光学
的な検出では、検出用の光をフローセルに集光させるこ
とができるため、高い検出感度が得られる。なお、結合
体の検出に際しては酵素活性の検出、放射線量の検出、
吸収・発光等の光学的検出等のために放射性同位元素、
色素、酵素等で標識された試薬が使用されることによ
り、結合体が標識されて検出される。In order to detect such a bound substance, a flow cell having a fine flow section is used. Particularly in the optical detection, since the detection light can be focused on the flow cell, high detection sensitivity can be obtained. When detecting the conjugate, detection of enzyme activity, detection of radiation dose,
Radioisotope for optical detection such as absorption and emission,
By using a reagent labeled with a dye, an enzyme or the like, the conjugate is labeled and detected.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら上述した
従来例では、フローセルの流通部の内径よりも大きい固
形物、担体の凝集体等が試料液に含まれると、これらに
よって流通部が閉塞されて担体が流れなくなり、或いは
流通部内の流れが乱れて測定誤差が大きくなるという問
題がある。However, in the above-mentioned conventional example, when the sample liquid contains a solid substance, an aggregate of carriers, etc., which is larger than the inner diameter of the flow section of the flow cell, the flow section is blocked by these and the carrier is blocked. Does not flow, or the flow in the distribution unit is disturbed, resulting in a large measurement error.
【０００５】本発明の目的は、上述した問題点を解消
し、流通部が閉塞することのないフローセルを提供する
ことにある。An object of the present invention is to solve the above-mentioned problems and to provide a flow cell in which the circulation part is not blocked.
【０００６】[0006]
【課題を解決するための手段】上述の目的を達成するた
めの本発明に係るフローセルは、試料液中の分析対象物
と結合する物質を坦持させた担体によって前記分析対象
物を検出するフローセルであって、前記試料液と前記担
体とを少なくとも含む反応液を受容する液溜部と、前記
担体を流す流通部とを有し、前記流通部の横断面の最大
内接円の直径よりも大きな直径を有する前記反応液中の
固形物を除去する除去手段を前記液溜部と前記流通部と
の間に設けたことを特徴とする。A flow cell according to the present invention for achieving the above object is a flow cell for detecting an analyte by a carrier carrying a substance that binds to the analyte in a sample solution. It has a liquid reservoir for receiving a reaction liquid containing at least the sample solution and the carrier, and a circulation part for flowing the carrier, and is larger than the diameter of the maximum inscribed circle of the cross section of the circulation part. It is characterized in that a removing means for removing a solid matter in the reaction solution having a large diameter is provided between the liquid reservoir section and the circulation section.
【０００７】[0007]
【作用】上述の構成を有する本発明に係るフローセル
は、反応液を受容する液溜部と、担体を流す流通部とを
有し、流通部の断面の最大内接円の直径よりも大きい固
形物を除去する除去手段を液溜部と流通部との間に設け
ることにより、反応液中の固形物を除去手段により除去
して担体のみを流通部へ流す。The flow cell according to the present invention having the above-mentioned structure has a liquid reservoir for receiving a reaction liquid and a flow portion for flowing a carrier, and is a solid larger than the diameter of the maximum inscribed circle of the cross section of the flow portion. By providing a removing means for removing the substance between the liquid reservoir and the circulation part, the solid substance in the reaction liquid is removed by the removing means, and only the carrier is flown into the circulation part.
【０００８】[0008]
【実施例】本発明を図示の実施例に基づいて詳細に説明
する。図１は第１の実施例の断面図であり、フローセル
１は最下層に基板２、中間層に中間体３、最上層に基板
４が接着されて成形されており、これらの基板２、中間
体３、基板４は透明材料によって製作されている。中間
体３には反応液を受容するための一方の液溜部５ａと、
反応液中の固形物を除去するための除去部６と、担体を
流すための流通部７が順次に形成され、基板４には液溜
部５ａと連通する液溜部５ｂが形成されている。このよ
うな液溜部５ａと液溜部５ｂとにより、反応液を収容す
るための液溜部５が形成されている。流通部７の流出口
にはＯリング８を介して吸引管９が取り付けられ、吸引
管９に図示しない吸引ポンプが接続されることにより、
担体が吸引ポンプにより吸引されて流通部７を流れるよ
うになっている。流通部７の上部には、例えば波長が４
８８ｎｍで出力が１５ｍＷのアルゴンレーザー装置１０
がレンズ１１を介して配置され、レーザー光が流通部７
に約１５μｍの径でスポット状に照射されるようになっ
ている。担体によって散乱された散乱光の進行方向には
レンズ１２、干渉フィルタ１３、光電子増倍管１４が配
置されている。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described in detail based on the illustrated embodiments. FIG. 1 is a cross-sectional view of the first embodiment, in which a flow cell 1 is formed by adhering a substrate 2 as the lowermost layer, an intermediate body 3 as the intermediate layer, and a substrate 4 as the uppermost layer. The body 3 and the substrate 4 are made of a transparent material. The intermediate body 3 has one liquid reservoir 5a for receiving the reaction liquid,
A removing section 6 for removing solids in the reaction solution and a circulation section 7 for flowing the carrier are sequentially formed, and a liquid reservoir 5b communicating with the liquid reservoir 5a is formed on the substrate 4. . The liquid reservoir 5a and the liquid reservoir 5b as described above form the liquid reservoir 5 for containing the reaction liquid. A suction pipe 9 is attached to the outlet of the circulation unit 7 via an O-ring 8, and a suction pump (not shown) is connected to the suction pipe 9,
The carrier is sucked by a suction pump and flows through the circulation unit 7. At the upper part of the circulation part 7, for example, a wavelength of 4
Argon laser device 10 with an output of 15 mW at 88 nm
Is arranged through the lens 11, and the laser light is distributed through the circulation part 7.
It is designed to be irradiated in spots with a diameter of about 15 μm. A lens 12, an interference filter 13, and a photomultiplier tube 14 are arranged in the traveling direction of the scattered light scattered by the carrier.
【０００９】レーザー光を流通部７に照射すると、担体
による散乱光はレンズ１２で集光され、干渉フィルタ１
３でレーザー光が除去された後に、光電子増倍管１４で
受光され、担体上の標識・色素から蛍光が測定されるよ
うになっている。When the laser light is applied to the flow section 7, the light scattered by the carrier is condensed by the lens 12, and the interference filter 1
After the laser light is removed in 3, the photomultiplier tube 14 receives the light, and the fluorescence is measured from the label / dye on the carrier.
【００１０】図２に示すように、中間体３は紫外線硬化
樹脂から圧縮成形法により形成され、除去部６の深さは
液溜部５ａ側で約５００μｍ、流通部７側で約１０μｍ
とされ、流通部７は中間体３の表面に形成されている。
基板２は厚さが２ｍｍのガラスとされ、中間体３の下に
接着される。基板４は図３に示すように厚さが２ｍｍの
ガラスとされ、中間体３の上に接着される。このように
形成された流通部７の断面の大きさは１０μｍ角、長さ
は２０ｍｍとされ、液溜部５の広さは５ｍｍ角、深さは
約２．５ｍｍとされ、容量はマイクロピぺット等で反応
液Ｌを扱うことができる０．１〜１０００μリットル程
度とされている。As shown in FIG. 2, the intermediate 3 is formed by compression molding from an ultraviolet curable resin, and the depth of the removing portion 6 is about 500 μm on the liquid reservoir 5a side and about 10 μm on the circulating portion 7 side.
Therefore, the circulation part 7 is formed on the surface of the intermediate body 3.
The substrate 2 is made of glass having a thickness of 2 mm and is bonded under the intermediate body 3. The substrate 4 is made of glass having a thickness of 2 mm as shown in FIG. 3, and is bonded onto the intermediate body 3. The cross-sectional size of the flow-through portion 7 thus formed is 10 μm square, the length is 20 mm, the width of the liquid reservoir 5 is 5 mm square, the depth is about 2.5 mm, and the capacity is micropipette. It is set to about 0.1 to 1000 μL so that the reaction liquid L can be handled with a tray or the like.
【００１１】液溜部５は試料液、担体、標識試薬等を含
む反応液を受容するものであるが、試料液、担体等の反
応槽としての機能や、反応液の希釈槽としての機能を備
えることもでき、機能に応じては複数の液溜部５を備え
ることもできる。The liquid reservoir 5 receives a reaction liquid containing a sample liquid, a carrier, a labeling reagent, etc., and has a function as a reaction tank for the sample liquid, the carrier, etc. and a function as a diluting tank for the reaction liquid. It may be provided, or a plurality of liquid reservoirs 5 may be provided depending on the function.
【００１２】除去部６は図４〜図６の原理説明図に示す
ように、一方の壁３ａと他方の壁４ａの間の間隔がＡ方
向からＢ方向へ一様に減少するように楔形に形成されて
いる。流通部７側の間隔DBは、担体Ｆの径Ｒよりも大き
く径Ｒの２倍よりも小さくされ、液溜部５側の間隔DAは
凝集体Ｇ、固形物Ｈ等が通ることができるように流通部
７側の間隔DBの数倍〜数１００倍程度とされている。こ
のような除去部６に試料液や担体Ｆを含む反応液Ｌが方
向Ａから方向Ｂに流されると、単一の担体Ｆは方向Ｂに
流れるが、凝集体Ｇや固形物Ｈはその大きさによって途
中で捕捉され、流通部７に流入することがなくなる。な
お、除去部６の両端部の開口の幅は、液溜部５側で流通
部７より狭くしても、或いはその逆にしてもよい。As shown in the principle explanatory diagrams of FIGS. 4 to 6, the removing portion 6 has a wedge shape so that the distance between the one wall 3a and the other wall 4a decreases uniformly from the A direction to the B direction. Has been formed. The distance DB on the side of the flow section 7 is larger than the diameter R of the carrier F and smaller than twice the diameter R, and the distance DA on the side of the liquid reservoir 5 allows the aggregates G, the solids H and the like to pass therethrough. In addition, it is set to several times to several hundred times the interval DB on the side of the distribution unit 7. When the reaction liquid L containing the sample liquid and the carrier F flows from the direction A to the direction B in such a removing section 6, the single carrier F flows in the direction B, but the aggregates G and the solids H have large sizes. As a result, it will not be caught in the middle of the flow and will not flow into the circulation unit 7. The width of the openings at both ends of the removing section 6 may be narrower on the liquid reservoir section 5 side than the circulation section 7 or vice versa.
【００１３】流通部７は担体Ｆを流すための細長い流路
とされ、ここに流れる担体Ｆを流通部７の一部で光学的
に測定することにより、試料液中の分析対象物を検出で
きるようになっている。流通部７の断面形状は担体Ｆの
径Ｒにより異なるが、ここでは円形又は正方形としてい
る。流通部７の大きさは、固形物Ｈや凝集体Ｇが流入す
ることを防止するために、担体Ｆの径Ｒよりも大きく、
担体Ｆの径Ｒの２倍より小さくする必要がある。流通部
７の長さは短か過ぎると流出入口が近接して担体Ｆの流
れに乱れが生じて光学部材の配置が難しくなってしま
い、長過ぎると流通部７の内壁と反応液Ｌとの抵抗が大
きくなって、反応液Ｌを流すために大きな駆動力が必要
となる。従って、流通部７の長さは５〜５０ｍｍ程度が
好適である。The flow section 7 is an elongated channel for flowing the carrier F. By optically measuring the carrier F flowing therein with a part of the flow section 7, the analyte in the sample solution can be detected. It is like this. Although the cross-sectional shape of the flow section 7 varies depending on the diameter R of the carrier F, it is circular or square here. The size of the flow portion 7 is larger than the diameter R of the carrier F in order to prevent the solid matter H and the aggregate G from flowing in,
It must be smaller than twice the diameter R of the carrier F. If the length of the flow section 7 is too short, the outflow port approaches and the flow of the carrier F is disturbed, which makes it difficult to dispose the optical member. If it is too long, the inner wall of the flow section 7 and the reaction solution L are separated from each other. The resistance increases and a large driving force is required to flow the reaction liquid L. Therefore, the length of the circulation portion 7 is preferably about 5 to 50 mm.
【００１４】このような構成によるフローセル１を用い
て分析対象物を分析するためには、例えばヒトＣＲＰ抗
体（ＩｇＧ分画）を物理的に吸着させた径Ｒが７μｍの
ポリスチレン粒子を担体Ｆとし、この分散液（粒子濃度
１０9 個／ｍリットル）１００μリットルと分析対象物
を含む試料液５００μリットルを５ｍリットルの容器内
で混合し、この混合液を３７℃で３０分間反応させる。
次に、反応液Ｌ中の担体Ｆを遠心分離法により沈降さ
せ、その上澄液を除去する。また、蛍光標識試薬である
ＦＩＴＣ標識をヒトＣＲＰ抗体が０．２ｍｇ／ｍリット
ルの濃度になるように、ｐＨ７．２の燐酸塩緩衝液−生
理食塩水つまり洗浄液に溶融した試薬１ｍリットルを加
え、３７℃で３０分間撹拌反応させ、遠心分離法を用い
て上澄液のみ除去する。更に、洗浄工程として洗浄液を
４ｍリットル加えて撹拌し、再び遠心分離法を用いて上
澄を除去し、この洗浄工程を３度繰り返す。最後に、洗
浄液を５００μリットル加えて担体Ｆを十分に分散させ
る。In order to analyze an object to be analyzed using the flow cell 1 having such a structure, for example, polystyrene particles having a diameter R of 7 μm to which a human CRP antibody (IgG fraction) is physically adsorbed are used as the carrier F. 100 μL of this dispersion (particle concentration 10 9 particles / mL) and 500 μL of a sample solution containing an analyte are mixed in a 5 mL container, and this mixed solution is reacted at 37 ° C. for 30 minutes.
Next, the carrier F in the reaction solution L is settled by a centrifugation method, and the supernatant is removed. In addition, 1 ml of the reagent, which was melted in the phosphate buffer-physiological saline of pH 7.2, that is, the washing solution, was added to the FITC label, which is a fluorescent labeling reagent, so that the human CRP antibody had a concentration of 0.2 mg / ml. The mixture is allowed to react with stirring at 37 ° C for 30 minutes, and only the supernatant is removed using a centrifugation method. Further, as a washing step, 4 ml of a washing solution is added and stirred, and the supernatant is removed again by using a centrifugal separation method, and this washing step is repeated 3 times. Finally, 500 μl of the washing liquid is added to sufficiently disperse the carrier F.
【００１５】このように適当に希釈した分散液を、液溜
部５に５０μリットルだけ滴下し、流通部７を通過させ
て担体Ｆを測定すると、蛍光が観測できると共に、除去
部６の存在により流通部７が閉塞することがない。When 50 μl of the appropriately diluted dispersion liquid is dropped into the liquid reservoir 5 and the carrier F is measured by passing through the flow portion 7, fluorescence can be observed and the presence of the removing portion 6 The distribution unit 7 will not be blocked.
【００１６】図７は第２の実施例の斜視図であり、ここ
では第１の実施例の除去部６の壁面３ａには段部３ｂが
数段階に形成されている。FIG. 7 is a perspective view of the second embodiment, in which the step portion 3b is formed in several steps on the wall surface 3a of the removing portion 6 of the first embodiment.
【００１７】上述した第１及び第２の実施例において、
フローセル１は流通部７で担体Ｆを光学的に測定でき、
かつ微細構造を容易に成形加工できるように無色透明な
ガラスや高分子材料等から形成しているが、特に熱可塑
性、熱硬化性、光硬化性等を有する種々の高分子材料か
ら射出成形、圧縮成形等を組合わせて形成すれば、低コ
ストで多量に形成することができる。また、シリコン等
の半導体材料の微細加工技術によっても形成でき、ガラ
スと高分子材料を組合わせても形成できる。また、反応
液Ｌの媒体と親和性の良い物質をフローセル１の内壁表
面に被覆すると、反応液Ｌがフローセル１内に侵入し易
くなる。このような被覆材には、例えば反応液Ｌの媒体
が水である場合には親水性物質、界面活性剤、メチルセ
ルロース、カルボキシメチルセルロース、ポリビニルア
ルコール、ポリアクリルアミド等の水溶性高分子を用い
ることができる。更に、除去部６にはメッシュ、紙、或
いはガラスから成るフィルタ等を用いることもできる
が、本実施例では除去部６の壁３ａと壁４ａの間隔を液
溜部５側から流通部７側に向けて徐々に狭くすることに
よりそれらの役目を持たせている。このような除去部６
はフローセル１を作成する際に作り込むことができるた
め、作成した後にメッシュやフィルタのような除去部６
を装着する場合に比べて手間が少なく、コストが低くな
る。In the first and second embodiments described above,
In the flow cell 1, the carrier F can be optically measured in the flow section 7,
And it is formed from colorless and transparent glass and polymer materials, etc. so that the microstructure can be easily molded and processed, but in particular injection molding from various polymer materials having thermoplasticity, thermosetting, photocurability, etc. If a combination of compression molding and the like is used, a large amount can be formed at low cost. It can also be formed by a fine processing technique of a semiconductor material such as silicon, or can be formed by combining glass and a polymer material. Further, when the inner wall surface of the flow cell 1 is coated with a substance having a good affinity with the medium of the reaction liquid L, the reaction liquid L easily enters the flow cell 1. For such a coating material, for example, when the medium of the reaction liquid L is water, a hydrophilic substance, a surfactant, a water-soluble polymer such as methyl cellulose, carboxymethyl cellulose, polyvinyl alcohol, polyacrylamide, etc. can be used. . Further, a filter made of mesh, paper, or glass may be used for the removing section 6, but in the present embodiment, the interval between the wall 3a and the wall 4a of the removing section 6 is changed from the liquid reservoir section 5 side to the circulation section 7 side. These roles are given by gradually narrowing toward. Such a removing unit 6
Can be created when the flow cell 1 is created, and therefore, the removal unit 6 such as a mesh or a filter can be created after the creation.
There is less labor and cost is lower than when wearing.
【００１８】なお、反応液Ｌを流通部７に流すための手
段として、実施例のように流通部７の流出口から反応液
Ｌを吸引するため吸引ポンプか、或いは液溜部５に注入
した反応液Ｌを加圧するための加圧ポンプが使用でき
る。また、例えば圧電素子を用いた電歪力や進行波を利
用して反応液Ｌを動かすような微小なポンプをフローセ
ル１内に内在させたり、流通部７の流入口の近傍に微小
なポンプを設けることができる。As a means for flowing the reaction liquid L into the circulation portion 7, a suction pump for sucking the reaction liquid L from the outlet of the circulation portion 7 as in the embodiment or the liquid reservoir 5 is injected. A pressurizing pump for pressurizing the reaction liquid L can be used. In addition, for example, a minute pump that moves the reaction liquid L using an electrostrictive force or a traveling wave using a piezoelectric element is provided inside the flow cell 1, or a minute pump is provided in the vicinity of the inlet of the circulation unit 7. Can be provided.
【００１９】[0019]
【発明の効果】以上説明したように本発明に係るフロー
セルは、除去手段によって試料液中の固形物を除去でき
るため、流通部が閉塞することがなくなり、担体が安定
して流れて、測定を良好に行うことができる。また、製
作する際に除去手段を同時に作り込めば、個別の除去手
段を装着する手間が不要になりコストが低くなる。As described above, in the flow cell according to the present invention, the solid matter in the sample liquid can be removed by the removing means, so that the flow section is not clogged, the carrier flows stably, and the measurement is performed. It can be done well. In addition, if the removing means are made at the same time during the production, the labor for mounting the individual removing means is unnecessary and the cost is reduced.
【図１】第１の実施例の断面図である。FIG. 1 is a sectional view of a first embodiment.
【図２】拡大斜視図である。FIG. 2 is an enlarged perspective view.
【図３】拡大斜視図である。FIG. 3 is an enlarged perspective view.
【図４】除去手段の原理を説明図である。FIG. 4 is a diagram illustrating the principle of a removing unit.
【図５】除去手段の原理を説明図である。FIG. 5 is an explanatory diagram of the principle of a removing unit.
【図６】除去手段の原理を説明図である。FIG. 6 is an explanatory diagram of the principle of a removing unit.
【図７】第２の実施例の斜視図である。FIG. 7 is a perspective view of a second embodiment.
１ フローセル ２、４ 基板 ３ 中間体 ５、５ａ、５ｂ 液溜部 ６ 除去部 ７ 流通部 1 Flow Cell 2, 4 Substrate 3 Intermediate 5, 5a, 5b Liquid Reservoir 6 Removal Part 7 Circulation Part
───────────────────────────────────────────────────── フロントページの続き (72)発明者 西村 松臣 東京都大田区下丸子三丁目目30番２号 キ ヤノン株式会社内 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Matsuomi Nishimura 3-30-2 Shimomaruko, Ota-ku, Tokyo Canon Inc.
Claims (2)
坦持させた担体によって前記分析対象物を検出するフロ
ーセルであって、前記試料液と前記担体とを少なくとも
含む反応液を受容する液溜部と、前記担体を流す流通部
とを有し、前記流通部の横断面の最大内接円の直径より
も大きな直径を有する前記反応液中の固形物を除去する
除去手段を前記液溜部と前記流通部との間に設けたこと
を特徴とするフローセル。1. A flow cell for detecting an analyte by a carrier carrying a substance that binds to the analyte in the sample liquid, which receives a reaction liquid containing at least the sample liquid and the carrier. A liquid reservoir having a liquid flow part and a flow part through which the carrier flows, and a removing means for removing solid matter in the reaction liquid having a diameter larger than the diameter of the maximum inscribed circle of the cross section of the liquid flow part. A flow cell provided between the reservoir and the circulation unit.
通部側に向けて一様又は段階的に縮径する空間とする請
求項１に記載のフローセル。2. The flow cell according to claim 1, wherein the removing means is a space in which the diameter is uniformly or stepwise reduced from the liquid reservoir side toward the flow portion side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3133093A JPH06222060A (en) | 1993-01-27 | 1993-01-27 | Flow cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3133093A JPH06222060A (en) | 1993-01-27 | 1993-01-27 | Flow cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06222060A true JPH06222060A (en) | 1994-08-12 |
Family
ID=12328252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3133093A Pending JPH06222060A (en) | 1993-01-27 | 1993-01-27 | Flow cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06222060A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11344497A (en) * | 1998-05-01 | 1999-12-14 | F Hoffmann La Roche Ag | Automatic analyzer |
| EP1062495A1 (en) * | 1998-03-07 | 2000-12-27 | Wardlaw, Stephen Clark | Calibration of a whole blood sample analyser |
| WO2004083823A1 (en) * | 2003-03-19 | 2004-09-30 | Nec Corporation | Microchip, sampling method, sample separating method, sample analyzing method, and sample recovering method |
| JP2007315879A (en) * | 2006-05-25 | 2007-12-06 | Matsushita Electric Ind Co Ltd | Device for optical analysis, and optical analyzer |
| WO2020085152A1 (en) * | 2018-10-23 | 2020-04-30 | 株式会社堀場製作所 | Duplex particle measuring device |
-
1993
- 1993-01-27 JP JP3133093A patent/JPH06222060A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1062495A1 (en) * | 1998-03-07 | 2000-12-27 | Wardlaw, Stephen Clark | Calibration of a whole blood sample analyser |
| EP1062495A4 (en) * | 1998-03-07 | 2008-01-09 | Wardlaw Stephen C | Calibration of a whole blood sample analyser |
| JPH11344497A (en) * | 1998-05-01 | 1999-12-14 | F Hoffmann La Roche Ag | Automatic analyzer |
| JP4553276B2 (en) * | 1998-05-01 | 2010-09-29 | エフ．ホフマン−ラ ロシュ アーゲー | Automatic analyzer |
| WO2004083823A1 (en) * | 2003-03-19 | 2004-09-30 | Nec Corporation | Microchip, sampling method, sample separating method, sample analyzing method, and sample recovering method |
| JP2007315879A (en) * | 2006-05-25 | 2007-12-06 | Matsushita Electric Ind Co Ltd | Device for optical analysis, and optical analyzer |
| JP4702182B2 (en) * | 2006-05-25 | 2011-06-15 | パナソニック株式会社 | Optical analysis device and optical analysis apparatus |
| WO2020085152A1 (en) * | 2018-10-23 | 2020-04-30 | 株式会社堀場製作所 | Duplex particle measuring device |
| JPWO2020085152A1 (en) * | 2018-10-23 | 2021-09-24 | 株式会社堀場製作所 | Duplex particle measuring device |
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