JPH06213893A - Prozone check method - Google Patents
Prozone check methodInfo
- Publication number
- JPH06213893A JPH06213893A JP733993A JP733993A JPH06213893A JP H06213893 A JPH06213893 A JP H06213893A JP 733993 A JP733993 A JP 733993A JP 733993 A JP733993 A JP 733993A JP H06213893 A JPH06213893 A JP H06213893A
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- prozone
- sample
- substance
- absorbance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、抗原抗体反応を利用し
た分析方法及び装置に関する。詳しくは、抗原抗体反応
におけるプロゾーン現象の判定と、この判定を自動的に
行うのに好適な自動分析装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analysis method and device utilizing an antigen-antibody reaction. Specifically, the present invention relates to a determination of prozone phenomenon in an antigen-antibody reaction and an automatic analyzer suitable for automatically performing this determination.
【０００２】[0002]
【従来の技術】病院等における臨床化学検査は、近年ま
すます自動化の方向にあり、生化学検査のみならず血清
検査の自動化も盛んになってきた。また各種疾患に関し
て臨床知見が蓄積されるにつれて、これらの疾患と密接
な関係を持つとされる物質が明らかにされつつある。こ
れら最近クローズアップされつつある物質は概して血清
中に微量にしか存在しないことが多い。そこで、これら
の物質は抗原抗体反応を利用して分析測定される。中で
も標識としてラジオアイソトープを用いる方法は微量成
分の測定に適している。また、免疫比濁法，ラテックス
凝集法などの免疫学的測定法も最近用いられるようにな
ってきた。2. Description of the Related Art In recent years, clinical chemistry tests in hospitals have become more and more automated, and not only biochemical tests but also serologic tests have become popular. Further, as clinical knowledge has been accumulated on various diseases, substances that are closely related to these diseases are being clarified. Often, these substances that have recently been highlighted have a very small amount in serum. Therefore, these substances are analyzed and measured by utilizing the antigen-antibody reaction. Above all, the method using a radioisotope as a label is suitable for measuring trace components. In addition, immunological measurement methods such as immunoturbidimetric method and latex agglutination method have recently been used.
【０００３】ところが、抗原抗体反応を利用するこれら
の分析法では、検体中に高濃度の抗体または抗原が含ま
れている場合に測定値が極めて低濃度を示すプロゾーン
現象（図２参照）がしばしば出現し、被検物質（抗原ま
たは抗体）の測定誤差をもたらしていた。このプロゾー
ン現象が起こっているか否かを判定する方法として、検
体を再添加又は希釈して同一検体を複数回測定する方法
（特開昭60−196669号，特開昭62−293163号）、時間を
ずらして２回同一検体を測定する方法（特開平3−11046
9 号）があるが、いずれも同一検体を複数回測定するも
のであった。したがって、自動分析装置が普及するにつ
れて単一操作内でプロゾーンを判定しうる方法が望まれ
ていた。However, in these analysis methods utilizing the antigen-antibody reaction, there is a prozone phenomenon (see FIG. 2) in which the measured value shows an extremely low concentration when the sample contains a high concentration of antibody or antigen. It often appeared and caused a measurement error of the test substance (antigen or antibody). As a method for determining whether or not the prozone phenomenon has occurred, a method of re-adding or diluting a sample and measuring the same sample multiple times (JP-A-60-196669, JP-A-62-293163), A method of measuring the same sample twice at different times (JP-A-3-11046).
No. 9), but all of them measured the same sample multiple times. Therefore, a method capable of determining the prozone within a single operation has been desired with the spread of automatic analyzers.
【０００４】[0004]
【発明が解決しようとする課題】上記従来技術では、高
価な試薬を倍以上使用しなければならず、プロゾーンチ
ェックのための時間が別に必要であった。In the above-mentioned prior art, an expensive reagent must be used more than twice, and a separate time for prozone check is required.
【０００５】本発明の目的は、抗原抗体反応を利用した
被検項目の分析測定に関して、プロゾーン現象によって
測定誤差が生じているか否かを単一操作内で容易に確認
できる判定方法及びその機能を有する分析装置を提供す
ることにある。An object of the present invention is to provide a determination method and its function which can easily confirm in a single operation whether or not a measurement error is caused by the prozone phenomenon in the analytical measurement of a test item using an antigen-antibody reaction. It is to provide an analyzer having the following.
【０００６】[0006]
【課題を解決するための手段】本発明は、検体中の被検
物質を抗原抗体反応を生ぜしめる反応物を含む液と検体
とを混合して、その反応過程を経時的に測定する間、被
検物質の定量に用いる測定点を含む任意の点で、プロゾ
ーン現象を判定しうる点を任意に定めて判定する方法と
その機能を有することを特徴とする自動分析装置を提供
することにより達成される。Means for Solving the Problems The present invention provides a method in which a test substance in a sample is mixed with a sample containing a reaction product that causes an antigen-antibody reaction, and the reaction process is measured over time. By providing an automatic analyzer characterized by having a function and a method of arbitrarily determining a point capable of determining a prozone phenomenon at any point including a measurement point used for quantifying a test substance To be achieved.
【０００７】[0007]
【作用】この発明では、たとえ見かけ上同一濃度と測定
されても、その反応過程はプロゾーン現象が起こってい
るか否かで異なることを利用して、プロゾーンチェック
を行うものなので、判定のために別の測定を行う必要が
なく、経済的で迅速な測定をおこなうことができる。In the present invention, the prozone check is performed by utilizing the fact that the reaction process varies depending on whether or not the prozone phenomenon occurs, even if the concentration is apparently the same. It is possible to perform economical and quick measurement without having to perform another measurement.
【０００８】[0008]
【実施例】以下、本発明の方法の実施に用いる測定装置
の一例を図１により説明する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An example of a measuring device used for carrying out the method of the present invention will be described below with reference to FIG.
【０００９】検体を入れた試料カップ１は、試料ディス
ク２の上に複数個設置される。試料ディスク２は、コン
ピュータ３によりインターフェース４を介して制御され
る。試料ディスク２は、あらかじめ登録された順番に従
って試料分注プローブ５の下まで回転移動し、試料は試
料分注プローブ５に連結された試料用ポンプ７により反
応容器６の中に所定量分注される。試料を分注された反
応容器６は恒温槽８に連絡された反応槽９の中を第一試
薬添加位置まで移動する。第一試薬添加位置まで移動し
た反応容器６は、試薬分注プローブ１０に連結された試
薬ポンプ１１により試薬ビン１２から吸引された所定の
第一試薬が加えられる。第一試薬添加後の反応容器６は
撹拌装置１３の位置まで移動最初の撹拌が行われる。内
容物が撹拌された反応容器６は光源１４から発した光束
を通過し、この時の吸光度は多波長光度計１５で検知さ
れる。検知された吸光度信号は、アナログ／デジタル
（Ａ／Ｄ）コンバータを経由し、インターフェイス４を
介してコンピュータ３に入り、試料中の測定対象濃度に
変換される。濃度変換されたデータは、インターフェイ
ス４を介してプリンタ１７から印字出力されるか、ＣＲ
Ｔ画面１８上に表示される。測定の終了した反応容器６
は洗浄機構１９の位置まで移動し、容器洗浄ポンプ２０
により内部の液を排出後水で洗浄され次の分析に供され
る。A plurality of sample cups 1 containing the sample are set on the sample disk 2. The sample disc 2 is controlled by the computer 3 via the interface 4. The sample disc 2 is rotationally moved to the bottom of the sample dispensing probe 5 according to a pre-registered order, and a predetermined amount of the sample is dispensed into the reaction container 6 by the sample pump 7 connected to the sample dispensing probe 5. It The reaction container 6 into which the sample has been dispensed moves to the first reagent addition position in the reaction tank 9 connected to the constant temperature tank 8. The reaction container 6 that has moved to the first reagent addition position is added with a predetermined first reagent sucked from the reagent bottle 12 by the reagent pump 11 connected to the reagent dispensing probe 10. After the addition of the first reagent, the reaction container 6 moves to the position of the stirring device 13 and the first stirring is performed. The reaction container 6 in which the contents are stirred passes through the light flux emitted from the light source 14, and the absorbance at this time is detected by the multi-wavelength photometer 15. The detected absorbance signal passes through the analog / digital (A / D) converter, enters the computer 3 through the interface 4, and is converted into the concentration to be measured in the sample. The density-converted data is printed out from the printer 17 via the interface 4 or the CR
It is displayed on the T screen 18. Reaction container 6 after measurement
Moves to the position of the cleaning mechanism 19, and the container cleaning pump 20
After discharging the liquid inside, it is washed with water and used for the next analysis.
【００１０】腫瘍マーカであるα−フェトプロテイン
（ＡＦＰ）を例として説明すると、図３に示す反応過程
では，Ａ点とＤ点の２点間における変化率を測定する方
法（レート法）を用いるため、見かけ上同一濃度になっ
てしまう。しかし、反応過程中のＢ点とＣ点の間の変化
率は、それぞれ異なっている。これは被検物質が大過剰
の場合、なだらかに吸光度が変化することが多いためで
ある。したがって、あらかじめ任意の２点と判定値を入
力しておき、コンピュータ３でのデータ処理時に判定値
と比較することによりプロゾーン現象が起こっているか
否かを判定する。このように、見かけ上同じに見えるデ
ータも、その反応過程の違いから区別することができる
のである。The tumor marker α-fetoprotein (AFP) will be described as an example. In the reaction process shown in FIG. 3, a method (rate method) for measuring the rate of change between two points A and D is used. , The density is apparently the same. However, the rate of change between points B and C during the reaction process is different. This is because when the test substance is in a large excess, the absorbance often changes gently. Therefore, it is determined whether or not the prozone phenomenon is occurring by inputting arbitrary two points and a judgment value in advance and comparing them with the judgment value during data processing in the computer 3. In this way, even data that appear to be the same can be distinguished from the difference in their reaction processes.
【００１１】以上本発明の実施例について説明したが、
本発明は上記実施例に限定されるものではなく、測定項
目や検体の違いに応じて適時変形実施可能であるのは言
うまでもない。The embodiments of the present invention have been described above.
It is needless to say that the present invention is not limited to the above-mentioned embodiment, and can be modified in a timely manner depending on the difference in measurement items and samples.
【００１２】[0012]
【発明の効果】本発明では、検体中の被検物質量を測定
するに当たり、従来法のような同一検体複数測定等を行
わずに済むので、自動分析装置に好適な経済的で迅速な
測定を行うことができるという効果がある。EFFECTS OF THE INVENTION In the present invention, when measuring the amount of a test substance in a sample, it is not necessary to perform multiple measurements of the same sample as in the conventional method, and therefore, an economical and rapid measurement suitable for an automatic analyzer. There is an effect that can be done.
【図１】本発明方法の実施に用いる測定装置の一例の全
体構成図である。FIG. 1 is an overall configuration diagram of an example of a measuring device used for carrying out a method of the present invention.
【図２】ＡＦＰのプロゾーン現象の説明図である。FIG. 2 is an explanatory diagram of a prozone phenomenon of AFP.
【図３】ＡＦＰの反応過程曲線図である。FIG. 3 is a reaction process curve diagram of AFP.
１…試料カップ、２…試料ディスク、３…コンピュー
タ、４…インターフェース、５…試料分注プローブ、６
…反応容器、７…試料用ポンプ、８…恒温槽、９…反応
槽、１０…試薬分注プローブ、１１…試薬ポンプ、１２
…試薬ビン、１３…撹拌装置、１４…光源、１５…多波
長光度計、１７…プリンタ、１８…ＣＲＴ画面、１９…
洗浄機構、２０…洗浄ポンプ。1 ... Sample cup, 2 ... Sample disk, 3 ... Computer, 4 ... Interface, 5 ... Sample dispensing probe, 6
... Reaction container, 7 ... Sample pump, 8 ... Constant temperature tank, 9 ... Reaction tank, 10 ... Reagent dispensing probe, 11 ... Reagent pump, 12
... Reagent bottle, 13 ... Stirrer, 14 ... Light source, 15 ... Multi-wavelength photometer, 17 ... Printer, 18 ... CRT screen, 19 ...
Washing mechanism, 20 ... Washing pump.
Claims (1)
質を含有する試薬とを混合し、その凝集生成物を含む反
応液に光を照射して、その吸光度や濁度を経時的に測定
することから、被検物質の定量を行う免疫分析方法にお
いて、反応過程におけるすべての測定点のうち任意の点
を判定用として定め、プロゾーンチェック現象が起こっ
ているか否か判定することを特徴とするプロゾーンチェ
ック方法。1. A test substance in a sample is mixed with a reagent containing a substance capable of antigen-antibody reaction, and the reaction solution containing the agglutination product is irradiated with light to measure its absorbance or turbidity with time. Therefore, in an immunoassay method for quantifying a test substance, an arbitrary point among all measurement points in the reaction process is set as a determination point to determine whether or not the prozone check phenomenon is occurring. Characteristic pro zone check method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP733993A JPH06213893A (en) | 1993-01-20 | 1993-01-20 | Prozone check method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP733993A JPH06213893A (en) | 1993-01-20 | 1993-01-20 | Prozone check method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06213893A true JPH06213893A (en) | 1994-08-05 |
Family
ID=11663190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP733993A Pending JPH06213893A (en) | 1993-01-20 | 1993-01-20 | Prozone check method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06213893A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2837937A1 (en) | 2013-08-15 | 2015-02-18 | Roche Diagniostics GmbH | Method for the detection of the prozone effect of photometric assays |
| JP2016031334A (en) * | 2014-07-30 | 2016-03-07 | 株式会社日立ハイテクノロジーズ | Analysis method and automatic analyzer |
-
1993
- 1993-01-20 JP JP733993A patent/JPH06213893A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2837937A1 (en) | 2013-08-15 | 2015-02-18 | Roche Diagniostics GmbH | Method for the detection of the prozone effect of photometric assays |
| CN105339794A (en) * | 2013-08-15 | 2016-02-17 | 豪夫迈·罗氏有限公司 | Method for the detection of the prozone effect of photometric assays |
| US10809258B2 (en) | 2013-08-15 | 2020-10-20 | Roche Diagnostics Operations, Inc. | Method for the detection of the prozone effect of photometric assays |
| JP2016031334A (en) * | 2014-07-30 | 2016-03-07 | 株式会社日立ハイテクノロジーズ | Analysis method and automatic analyzer |
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