JPH06153902A - Culture tank - Google Patents

Culture tank

Info

Publication number
JPH06153902A
JPH06153902A JP33364392A JP33364392A JPH06153902A JP H06153902 A JPH06153902 A JP H06153902A JP 33364392 A JP33364392 A JP 33364392A JP 33364392 A JP33364392 A JP 33364392A JP H06153902 A JPH06153902 A JP H06153902A
Authority
JP
Japan
Prior art keywords
culture
culture bag
bag
lid
culture tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP33364392A
Other languages
Japanese (ja)
Inventor
Yoshishige Ashihara
義重 芦原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHIBATA HARIO GLASS KK
Original Assignee
SHIBATA HARIO GLASS KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHIBATA HARIO GLASS KK filed Critical SHIBATA HARIO GLASS KK
Priority to JP33364392A priority Critical patent/JPH06153902A/en
Publication of JPH06153902A publication Critical patent/JPH06153902A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To inexpensively provide a culture tank having ready handleability because of lightness and flexibility. CONSTITUTION:A main body of a culture tank to be charged with a culture solution L consists of a bag 10 made of a flexible synthetic resin capable of standing high temperature and high pressure of vapor sterilization. A top open part 10a of the culture bag 10 is put on a fitting part 11a of a cover 11 and the fitting part 11a is fastened with a detachable band 12 and hermetically fixed. The cover 11 is held on supporting legs 13 and the culture bag 10 is suspended. The cover 11 is equipped with a stirring tool 3, a porous ventilating pipe 4 and a through hole 11b for inserting a dissolved oxygen detecting sensor 5 into the culture bag 10. Since the culture bag 10 has lightness and flexibility, the culture bag can be folded and stored during nonuse and is not readily damaged by physical impact and is conveniently handled. Facilities for sterilization are sufficiently of small size. A culture bag made of a synthetic resin on the market can be widely applied to the culture bag 10.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、微生物、動植物等を液
体培養するための培養槽に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture tank for liquid culture of microorganisms, animals and plants.

【０００２】[0002]

【従来の技術】従来の培養槽は、図２に示すように、培
養液Ｌを収容する透明のガラス容器１に、蓋体２が気密
に嵌合している。蓋体２には、例えば攪拌具３、螺旋状
の多孔質通気管４、溶存酸素検出センサ５などが差し込
まれており、酸素濃度等の培養に必要な環境条件を調整
することができるようになっている。その他従来の培養
槽としては、プラスチックあるいはステンレススチール
等の金属製の容器を用いたものが知られている。2. Description of the Related Art In a conventional culture tank, as shown in FIG. 2, a lid 2 is airtightly fitted to a transparent glass container 1 containing a culture solution L. A stirring tool 3, a spiral porous ventilation tube 4, a dissolved oxygen detection sensor 5 and the like are inserted in the lid body 2 so that environmental conditions such as oxygen concentration necessary for culturing can be adjusted. Has become. In addition, as a conventional culture tank, one using a container made of metal such as plastic or stainless steel is known.

【０００３】[0003]

【発明が解決しようとする課題】上記従来の培養槽は、
何れも容器の加工のために比較的高価になってしまう
し、大型になると体積や重量も大きくなって取扱いが不
便である。培養前に、容器をオートクレーブにて高圧蒸
気滅菌するが、容器が大型大重量化すれば、滅菌設備も
大型、堅牢化せざるを得ず、大きな設備装置を必要とす
るという問題がある。そこで、本発明は、軽量で伸縮自
在であるために取扱いが容易な培養槽を安価に提供する
ことを課題としている。The above-mentioned conventional culture tank is
All of them are relatively expensive due to the processing of the container, and when they are large in size, the volume and weight are large, which makes the handling inconvenient. Before culturing, the container is sterilized by autoclave under high pressure steam. However, if the container becomes large and heavy, the sterilization equipment must be large and robust, and a large equipment is required. Therefore, an object of the present invention is to provide a culture tank which is lightweight and expandable and easy to handle at low cost.

【０００４】[0004]

【課題を解決するための手段】上記課題を解決するた
め、本発明は、柔軟な材質から成り、内部に培養液Ｌを
収容することができる培養袋１０と、培養袋１０の開放
部１０ａを着脱可能に閉鎖するための蓋体１１と、培養
袋１０を吊支する支持部材１３とから培養槽を構成し
た。この培養槽において、蓋体１１を支持部材１３の上
部に支持し、培養袋１０の上部開放部１０ａを蓋体１１
に着脱自在に係止した。蓋体１１に、培養袋１０内へ接
続される諸装置を導入するための貫通孔１１ｂを設け
た。培養袋１０の上部開放部１０ａを、蓋体１１の周囲
に気密に装着した。培養袋１０を、高圧蒸気滅菌処理の
高温高圧に耐えることができる材質で構成した。In order to solve the above problems, the present invention comprises a culture bag 10 made of a flexible material and capable of containing a culture solution L therein, and an opening portion 10a of the culture bag 10. A culture tank was composed of a lid 11 for detachably closing and a support member 13 for suspending and supporting the culture bag 10. In this culture tank, the lid 11 is supported on the support member 13, and the upper opening 10a of the culture bag 10 is attached to the lid 11.
Removably locked to. The lid 11 was provided with a through hole 11b for introducing various devices connected to the inside of the culture bag 10. The upper open portion 10a of the culture bag 10 was airtightly mounted around the lid 11. The culture bag 10 was made of a material capable of withstanding the high temperature and high pressure of the high pressure steam sterilization treatment.

【０００５】[0005]

【作用】本発明の培養槽は、培養袋１０が柔軟であるか
ら、折り畳むことができ、運搬、保管などの面で取扱い
が容易である。特に、培養前の滅菌処理を、培養袋１０
を蓋体１１から取り外して収縮させた状態で行うことが
できる。この場合には、培養袋１０の容量に比してかな
り小さな滅菌処理設備で足りる。培養袋１０は市販の合
成樹脂製のものを広く適用することができ、培養液Ｌの
種類に応じて適宜選択し、取り替え使用する。培養袋１
０の取り替えは簡単である。In the culture tank of the present invention, since the culture bag 10 is flexible, it can be folded and is easy to handle in terms of transportation and storage. In particular, the sterilization process before culturing is performed by the culture bag 10.
Can be performed in a contracted state by removing from the lid 11. In this case, a sterilization facility which is considerably smaller than the capacity of the culture bag 10 is sufficient. A wide variety of commercially available synthetic resin products can be used as the culture bag 10, and the culture bag 10 is appropriately selected according to the type of the culture solution L and used for replacement. Culture bag 1
The replacement of 0 is easy.

【０００６】[0006]

【実施例】本発明の実施例を図面について説明する。図
１において、１０は培養体を含む培養液Ｌを収容した培
養袋である。培養袋１０は蒸気滅菌の高温高圧に耐え得
る厚さ０.５mmの透明のビニル製である。従って、折り
畳みが可能な柔軟性を有する。培養袋１０の上部の開放
部１０ａは蓋体１１に係止されている。蓋体１１は、円
盤状のステンレススチール板である。蓋体１１の下面に
は、短円筒状を成し、端部に鍔を有する取付部１１ａを
備えている。培養袋１０は取付部１１ａの外側に開放部
１０ａをかぶせて鍔と円盤下面との間にバンド１２にて
気密に締め止められる。蓋体１１は、攪拌具３、多孔質
通気管４、溶存酸素検出センサ５を培養袋１０内に挿入
するための貫通孔１１ｂを備えている。バンド１２は着
脱自在である。蓋体１１はその縁部を支持部材である支
持脚１３で支持されている。支持脚１３の上部は蓋体１
１を固定して、培養袋１０を内部に垂下せしめている。Embodiments of the present invention will be described with reference to the drawings. In FIG. 1, 10 is a culture bag containing a culture solution L containing a culture. The culture bag 10 is made of transparent vinyl with a thickness of 0.5 mm that can withstand the high temperature and pressure of steam sterilization. Therefore, it has the flexibility such that it can be folded. The open portion 10a at the top of the culture bag 10 is locked to the lid 11. The lid 11 is a disc-shaped stainless steel plate. The lower surface of the lid 11 is provided with a mounting portion 11a having a short cylindrical shape and having a brim at its end. The culture bag 10 is airtightly fastened with a band 12 between the brim and the lower surface of the disk by covering the attachment portion 11a with an open portion 10a. The lid 11 is provided with a through hole 11b for inserting the stirring tool 3, the porous ventilation tube 4, and the dissolved oxygen detection sensor 5 into the culture bag 10. The band 12 is removable. The edge of the lid 11 is supported by support legs 13 which are support members. The top of the support leg 13 is the lid 1
1 is fixed and the culture bag 10 is hung inside.

【０００７】この培養槽においては、培養袋１０の開放
部１０ａを蓋体１１の取付部１１ａの外側にかぶせてバ
ンド１２で締め付けることにより培養袋１０を蓋体１１
に気密に取り付ける。培養袋１０は、不使用時に蓋体１
１から外して折り畳んで置けば保管スペースを取らない
し、軽量で、柔軟であるから、落下等による物理的衝撃
が加わってもそれ自身容易に破損することがなく、また
他の物を破損させたりすることもない。従って、運搬、
保管などの面で取扱いに便利である。また、培養袋１０
を蓋体１１から取り外した状態でまるめて収縮させれ
ば、比較的小さなオートクレーブ装置でも複数を一度に
高圧蒸気滅菌することができる。このように滅菌処理し
た培養袋１０を装着して、培養槽を組み立てた後に、別
に濾過滅菌した培養液Ｌを注入すればよいから、処理が
簡単である。培養袋１０は市販の合成樹脂製のものを広
く適用することができ、培養液Ｌの種類に応じて適宜変
更することができ、その際の取り替えが簡単である。In this culture tank, the culture bag 10 is covered with the open portion 10a of the culture bag 10 by covering the open portion 10a of the culture bag 10 on the outside of the attachment portion 11a of the lid 11 and tightening the band 12.
Attach airtightly to. The culture bag 10 is a lid 1 when not in use.
If you remove it from 1 and fold it, it will not take up storage space, and because it is lightweight and flexible, it will not easily break itself even if it is subjected to physical impact such as falling, and it will also damage other things. There is nothing to do. Therefore, transportation,
It is convenient to handle in terms of storage. Also, the culture bag 10
If a plurality of is removed from the lid body 11 and is completely contracted, even a relatively small autoclave device can perform high-pressure steam sterilization at one time. Since the culture bag 10 thus sterilized is mounted and the culture tank is assembled, the culture solution L sterilized by filtration may be separately injected, so that the treatment is simple. A wide variety of commercially available synthetic resin bags can be used as the culture bag 10, and the culture bag 10 can be appropriately changed according to the type of the culture solution L, and replacement at that time is easy.

【０００８】[0008]

【発明の効果】以上のように、本発明は、柔軟な材質か
ら成り、内部に培養液Ｌを収容することができる培養袋
１０と、培養袋１０の開放部１０ａを着脱可能に閉鎖す
るための蓋体１１と、培養袋１０を吊支する支持部材１
３とから培養槽を構成したため、培養袋が軟質材である
から、容量が大きくても不使用時に折り畳んでおける
し、重量も軽く、保管、運搬等の面で取扱いに便利であ
る。特に、培養前の滅菌処理も比較的小さな設備で簡単
に行うことができる。培養袋は市販の合成樹脂製のもの
を広く適用することができるから、安価に提供すること
ができるという効果を有する。INDUSTRIAL APPLICABILITY As described above, according to the present invention, the culture bag 10 made of a flexible material and capable of containing the culture liquid L therein and the opening portion 10a of the culture bag 10 are detachably closed. Support member 1 for suspending and supporting the culture bag 10
Since the culture tank is composed of 3 and the culture bag is a soft material, it can be folded even when it is not in use even if it has a large capacity, and the weight is light, and it is convenient in terms of storage and transportation. In particular, the sterilization process before culturing can be easily performed with a relatively small facility. Since the culture bag made of synthetic resin on the market can be widely applied, it has an effect that it can be provided at a low cost.

【図面の簡単な説明】[Brief description of drawings]

【図１】本発明に係る培養槽の縦断面図である。FIG. 1 is a vertical sectional view of a culture tank according to the present invention.

【図２】従来の培養槽の縦断面図である。FIG. 2 is a vertical sectional view of a conventional culture tank.

【符号の説明】[Explanation of symbols]

３ 攪拌具 ４ 多孔質通気管 ５ 溶存気体検出装置 １０ 培養袋 １０ａ 開放部 １１ 蓋体 １１ｂ 貫通孔 １３ 支持部材 Ｌ 培養液 3 Stirrer 4 Porous Venting Tube 5 Dissolved Gas Detector 10 Culture Bag 10a Opening Part 11 Lid 11b Through Hole 13 Supporting Member L Culture Solution

Claims (5)

【特許請求の範囲】[Claims] 【請求項１】 柔軟な材質から成り、内部に培養液を収
容することができる培養袋と、培養袋の開放部を着脱可
能に閉鎖するための蓋体と、培養袋を吊支する支持部材
とを具備することを特徴とする培養槽。1. A culture bag made of a flexible material capable of containing a culture solution therein, a lid for detachably closing an opening of the culture bag, and a support member for suspending and supporting the culture bag. A culture tank comprising: 【請求項２】 前記蓋体が前記支持部材の上部に支持さ
れ、前記培養袋の上部開放部が蓋体に着脱自在に係止さ
れていることを特徴とする請求項１に記載の培養槽。2. The culture tank according to claim 1, wherein the lid is supported on an upper portion of the support member, and an upper opening portion of the culture bag is detachably engaged with the lid. . 【請求項３】 前記蓋体は、前記培養袋内へ接続される
諸装置を導入するための貫通孔を備えていることを特徴
とする請求項１に記載の培養槽。3. The culture tank according to claim 1, wherein the lid body has a through hole for introducing various devices connected to the inside of the culture bag. 【請求項４】 前記培養袋の上部開放部は、蓋体の周囲
に気密に装着されることを特徴とする請求項１に記載の
培養槽。4. The culture tank according to claim 1, wherein the upper opening portion of the culture bag is airtightly mounted around the lid body. 【請求項５】 前記培養袋は、高圧蒸気滅菌処理の高温
高圧に耐えることができる材質であることを特徴とする
請求項１に記載の培養槽。5. The culture tank according to claim 1, wherein the culture bag is made of a material that can withstand the high temperature and high pressure of high pressure steam sterilization.
JP33364392A 1992-11-19 1992-11-19 Culture tank Withdrawn JPH06153902A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP33364392A JPH06153902A (en) 1992-11-19 1992-11-19 Culture tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP33364392A JPH06153902A (en) 1992-11-19 1992-11-19 Culture tank

Publications (1)

Publication Number Publication Date
JPH06153902A true JPH06153902A (en) 1994-06-03

Family

ID=18268354

Family Applications (1)

Application Number Title Priority Date Filing Date
JP33364392A Withdrawn JPH06153902A (en) 1992-11-19 1992-11-19 Culture tank

Country Status (1)

Country Link
JP (1) JPH06153902A (en)

Cited By (15)

* Cited by examiner, † Cited by third party
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WO2005021703A1 (en) * 2003-08-29 2005-03-10 Yamaha Hatsudoki Kabushiki Kaisha Microorganism culturing apparatus and method of inserting bag into culturing apparatus
US8123199B2 (en) 2004-01-07 2012-02-28 Atmi Packaging, Inc. Bioreactor
US8182137B2 (en) 2000-10-09 2012-05-22 Atmi Packaging, Inc. Mixing bag or vessel with a fluid-agitating element
US8282269B2 (en) 2000-10-09 2012-10-09 Atmi Packaging, Inc. Mixing bag or vessel having a fluid-agitating element
US8282267B2 (en) 2006-10-03 2012-10-09 Artelis S.A. Mixing system including a flexible bag, specific flexible bag and locating system for the mixing system
US9005971B2 (en) 2005-04-22 2015-04-14 Life Technologies Corporation Gas spargers and related container systems
WO2015077663A1 (en) * 2013-11-21 2015-05-28 Distek Inc. Disposable bioreactors and methods for construction and use thereof
KR20160061128A (en) * 2014-11-21 2016-05-31 주식회사 지디이 Culturing apparatus of light agitation type
US9643133B2 (en) 2011-09-30 2017-05-09 Life Technologies Corporation Container with film sparger
JP2017127226A (en) * 2016-01-19 2017-07-27 大成建設株式会社 Culture method of microorganisms for environmental purification used in natural environment, and environmental purification method
EP2044190B1 (en) * 2006-06-16 2017-09-13 GE Healthcare Bio-Sciences Corp. Gas delivery configurations, foam control systems, and bag molding methods and articles for collapsible bag vessels and bioreactors
US10123940B2 (en) 2014-06-26 2018-11-13 Advanced Scientific, Inc. Bag assembly and system for use with a fluid
US10301585B2 (en) 2011-09-29 2019-05-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
US10589197B2 (en) 2016-12-01 2020-03-17 Life Technologies Corporation Microcarrier filter bag assemblies and methods of use
EP2284253B2 (en) † 1999-09-08 2020-05-27 Levitronix Technologies, LLC Bioreactor

Cited By (30)

* Cited by examiner, † Cited by third party
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EP2284253B2 (en) † 1999-09-08 2020-05-27 Levitronix Technologies, LLC Bioreactor
US8182137B2 (en) 2000-10-09 2012-05-22 Atmi Packaging, Inc. Mixing bag or vessel with a fluid-agitating element
US8282269B2 (en) 2000-10-09 2012-10-09 Atmi Packaging, Inc. Mixing bag or vessel having a fluid-agitating element
WO2005021703A1 (en) * 2003-08-29 2005-03-10 Yamaha Hatsudoki Kabushiki Kaisha Microorganism culturing apparatus and method of inserting bag into culturing apparatus
US8123199B2 (en) 2004-01-07 2012-02-28 Atmi Packaging, Inc. Bioreactor
US9475012B2 (en) 2005-04-22 2016-10-25 Life Technologies Corporation Gas spargers and related container systems
US10328404B2 (en) 2005-04-22 2019-06-25 Life Technologies Corporation Gas spargers and related container systems
US9005971B2 (en) 2005-04-22 2015-04-14 Life Technologies Corporation Gas spargers and related container systems
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