JPH06102152A - Standard solution for flow type particle picture analyzing instrument - Google Patents
Standard solution for flow type particle picture analyzing instrumentInfo
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- JPH06102152A JPH06102152A JP24953692A JP24953692A JPH06102152A JP H06102152 A JPH06102152 A JP H06102152A JP 24953692 A JP24953692 A JP 24953692A JP 24953692 A JP24953692 A JP 24953692A JP H06102152 A JPH06102152 A JP H06102152A
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、流れている液体中に懸
濁した粒子の画像を撮り、粒子を分析する粒子画像分析
装置に関するものであり、特に血液または尿中の細胞や
粒子を分析するのに適した粒子画像分析装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a particle image analyzer for taking an image of particles suspended in a flowing liquid and analyzing the particles, and particularly for analyzing cells or particles in blood or urine. The present invention relates to a particle image analysis device suitable for
【０００２】[0002]
【従来の技術】血液中の細胞や尿中の細胞や粒子を分類
分析するには、従来、スライドガラス上に標本を作成し
顕微鏡にて観察することで行われてきた。尿の場合に
は、尿中の粒子濃度が薄いため、サンプルを予め遠心分
離器で遠心濃縮してから観察している。2. Description of the Related Art Classification and analysis of cells in blood and cells and particles in urine have hitherto been carried out by preparing a sample on a slide glass and observing with a microscope. In the case of urine, since the particle concentration in urine is low, the sample is observed after being centrifugally concentrated by a centrifugal separator.
【０００３】これらの観察，検査の作業を自動化する装
置としては、血液などのサンプル試料をスライドガラス
上に塗沫し顕微鏡にセットし、顕微鏡ステージを自動的
に走査し粒子の存在する位置で粒子の静止画像を撮影
し、画像処理技術による特徴抽出およびパターン認識手
法を用い、サンプル試料中にある粒子の分類等を行って
いる。As an apparatus for automating these observation and inspection operations, a sample sample such as blood is smeared on a slide glass and set on a microscope, and the microscope stage is automatically scanned to move particles at the position where particles are present. The still image of the above is photographed, and the feature extraction by the image processing technology and the pattern recognition method are used to classify the particles in the sample.
【０００４】しかし、上記手法では標本作成に時間がか
かること、さらに顕微鏡ステージを機械的に移動しなが
ら粒子を見つけ、粒子を適当な画像取り込み領域へ移動
させる作業が必要である。そのため、分析に時間を要し
たり、機械機構が複雑になる問題がある。However, in the above method, it takes time to prepare a sample, and it is necessary to find particles while mechanically moving the microscope stage and move the particles to an appropriate image capturing area. Therefore, there is a problem that analysis takes time and a mechanical mechanism becomes complicated.
【０００５】塗沫標本を作成せず、サンプル試料を液体
中に懸濁させたままフローセル中を流し、光学的に分析
するフローサイトメータ方法が知られている。フローサ
イトメータによる方法は、サンプル中の粒子１個１個か
らの蛍光強度や散乱光強度を観測するもので、毎秒数１
０００個の処理能力を持っている。しかし、粒子の形態
学的特徴を反映する特徴量を観測することはむずかし
く、形態学的特徴で粒子を分類することがむずかしい。There is known a flow cytometer method in which a smear sample is not prepared, and a sample sample is allowed to flow in a flow cell while being suspended in a liquid and optically analyzed. The method using a flow cytometer is to observe the fluorescence intensity and the scattered light intensity from each particle in the sample.
It has a processing capacity of 000. However, it is difficult to observe the feature quantity that reflects the morphological characteristics of the particles, and it is difficult to classify the particles based on the morphological characteristics.
【０００６】連続的に流れているサンプル試料中の粒子
画像を撮影し、個々の粒子画像から粒子を分析・分類す
る試みとしては、特公昭57−500995号，特開昭63−9415
6 号等が知られている。As an attempt to photograph particle images in a continuously flowing sample sample and analyze and classify the particles from the individual particle images, JP-B-57-500995 and JP-A-63-9415 are known.
No. 6 is known.
【０００７】特公昭57−500995号では、サンプル試料を
特別な形状の流路に通し、そこで試料中の粒子を幅広の
撮像領域中を流し、フラッシュランプによる静止画像を
撮影し、その画像を用い粒子分析する方法が示されてい
る。In Japanese Examined Patent Publication No. 57-500995, a sample sample is passed through a channel having a special shape, particles in the sample are caused to flow through a wide imaging region, a still image is taken by a flash lamp, and the image is used. A method for particle analysis is shown.
【０００８】特開昭63−94156 号では、静止画像撮影系
とは別にサンプル流れ中の粒子画像撮影領域より上流に
粒子検出用光学系を有している。あらかじめ粒子検出部
で粒子通過を知り、丁度その粒子が粒子画像撮影領域に
達したとき適当なタイミングでフラッシュランプを点灯
させる方法である。この方法では、パルス光源の発光を
周期的に行わず粒子の通過を検出してその時だけタイミ
ングを合わせて静止画像を撮影することができ、効率的
な粒子画像を集められ、濃度の薄いサンプル試料の場合
でも無意味な画像を処理することはない。In Japanese Patent Laid-Open No. 63-94156, an optical system for particle detection is provided upstream of the particle image capturing area in the sample flow, in addition to the still image capturing system. This is a method in which the passage of particles is known in advance by the particle detection unit and the flash lamp is turned on at an appropriate timing when the particles have just reached the particle image capturing area. With this method, it is possible to detect the passage of particles without periodically emitting light from the pulsed light source, and to capture a still image at the same timing only at that time. Even in case of meaningless image is not processed.
【０００９】このとき装置調整用の標準液はヒトやニワ
トリの固定化赤血球、あるいはラテックス粒子を希釈し
たものが一般的であり、フローサイトメータ方法の粒子
検出機構系の補正、並びに画像処理系の更正用粒子とし
て使用されている。At this time, the standard solution for adjusting the apparatus is generally a solution of human or chicken fixed erythrocytes or diluted latex particles. The standard solution for the flow cytometer method is used to correct the particle detection mechanism system and the image processing system. Used as calibrating particles.
【００１０】[0010]
【発明が解決しようとする課題】しかしながら、上記従
来技術の標準液には次のような問題点がある。However, the above-mentioned conventional standard solutions have the following problems.
【００１１】１．ヒトやニワトリの固定化赤血球は採血
検体の固体差があり、安定した品質の供給が困難である
と共に、同一検体からの供給量にも限度がある。1. Fixed erythrocytes of humans and chickens have individual differences in blood samples, and it is difficult to supply stable quality, and the amount supplied from the same sample is also limited.
【００１２】２．また、生体試料であるため病原菌に感
染する危険性や要冷蔵保存など取扱条件が煩雑である。2. Further, since it is a biological sample, handling conditions such as a risk of being infected with pathogenic bacteria and refrigerating storage are complicated.
【００１３】３．ラテックス粒子は一般に染色不可能な
ため染色液の管理が行えず、総合的な画像処理系の更正
粒子には不適である。3. Since latex particles cannot generally be dyed, the dyeing solution cannot be controlled, and they are unsuitable for calibrating particles in an integrated image processing system.
【００１４】４．また、高価格であるが染色可能な粒子
はさらに高価格である。4. Also, although the price is high, dyeable particles are even more expensive.
【００１５】従って本発明の目的は、粒子検出系を伴っ
た粒子画像撮像系を構築する上で、標準粒子のロットサ
イズが大きいこと，ロット内，ロット間のバラツキが小
さいこと，感染性がないこと，長期保存が可能で取扱が
容易なこと，サイズが一定で形状が均一なこと，染色液
による染色が可能なこと、そして安価であることなどの
条件を満たした標準液を提供することである。Therefore, an object of the present invention is to construct a particle image pickup system with a particle detection system, in which a lot size of standard particles is large, variation within a lot and between lots is small, and there is no infectivity. By providing a standard solution that satisfies the conditions that it can be stored for a long period of time and is easy to handle, that it has a uniform size and uniform shape, that it can be dyed with a dyeing solution, and that it is inexpensive. is there.
【００１６】また、上記標準粒子のサイズや形状，染色
性の異なるものを数種類混ぜ合わせ、より詳細な染色性
の検討，メンテナンス性の向上をはかれる標準液を提供
することである。It is another object of the present invention to provide a standard solution in which several kinds of the standard particles having different sizes, shapes, and dyeing properties are mixed to study the dyeing properties in more detail and improve the maintainability.
【００１７】[0017]
【課題を解決するための手段】上記目的を達成するため
にラテックス粒子より安価で染色可能な標準粒子を調査
した結果、イオン交換樹脂が １．粒子サイズは血球の代用となる１０〜１５μｍ、細
胞の代用となる２５〜５０μｍのものを選択できること ２．ラテックス粒子に比べ価格が安いこと ３．染色液による染色が可能で、かつ容液のｐＨを変え
ることで染色後の色調を調整できること ４．形状が球形で、粒度分布の狭いこと ５．形状の加工が可能であること など、好条件を備えていることが明らかとなった。In order to achieve the above object, as a result of investigating standard particles which are cheaper than latex particles and can be dyed, it was found that the ion exchange resin was 1. The particle size can be selected to be 10 to 15 μm which is a substitute for blood cells and 25 to 50 μm which is a substitute for cells. The price is cheaper than latex particles. 3. Capable of dyeing with a dyeing solution and adjusting the color tone after dyeing by changing the pH of the solution. 4. Spherical shape with narrow particle size distribution It has become clear that it has favorable conditions such as the ability to process the shape.
【００１８】そこで標準粒子にイオン交換樹脂を採用す
ることにより、粒子の存在頻度から粒子検出機構系の補
正を、サイズが均一で染色可能であることから粒子検出
系と画像処理系の補正を、染色可能なことから画像処理
系の補正と染色液の品質管理を、形状が扁平であること
から測定サンプルの流れの制御を安定に行うものであ
る。Therefore, by adopting an ion exchange resin for the standard particles, the correction of the particle detection mechanism system is performed based on the existence frequency of the particles, and the correction of the particle detection system and the image processing system is performed because the size is uniform and dyeing is possible. Since the dyeing is possible, the correction of the image processing system and the quality control of the dyeing solution are performed, and the flat shape allows the flow of the measurement sample to be stably controlled.
【００１９】[0019]
【作用】ラテックス粒子より安価で染色可能なイオン交
換樹脂を標準粒子として採用することにより、病原菌に
よる感染性がなくなると同時に長期保存が可能になるな
ど取扱いが容易となる。さらに上記標準粒子を用いて、 １．粒子が高濃度で均一な標準液の調製をすることで、
流路の位置調整，粒子検出のタイミング，流速調整，フ
ォーカス調整が容易になる。By using an ion exchange resin, which is cheaper than latex particles and can be dyed, as standard particles, the infectivity by pathogenic bacteria is eliminated, and at the same time, long-term storage is possible and handling becomes easy. Furthermore, using the above standard particles, 1. By preparing a standard solution with high concentration of particles,
It facilitates flow path position adjustment, particle detection timing, flow velocity adjustment, and focus adjustment.
【００２０】２．サイズを選択したり粒子の染色性の違
いを組み合わせることで、粒子画像解析の分類アルゴリ
ズムのチェックが可能になる。2. It is possible to check the classification algorithm for particle image analysis by selecting the size and combining the differences in particle dyeability.
【００２１】３．粒子の形状を扁平で細長いものを選択
することで、フロー制御が容易になる。3. Flow control is facilitated by selecting a flat and elongated particle shape.
【００２２】４．粒子の染色性をチェックすることで、
染色液の品質管理が容易となる。4. By checking the dyeability of the particles,
The quality control of the dyeing solution becomes easy.
【００２３】など、各種メンテナンスが迅速かつ容易に
実施できるようになる。Various maintenances can be performed quickly and easily.
【００２４】[0024]
【実施例】以下、本発明の実施例を図１を用いて説明す
る。EXAMPLE An example of the present invention will be described below with reference to FIG.
【００２５】図１は本発明による標準液が適用されるフ
ロー式粒子画像解析装置の概要である。１はサンプル容
器、２はサンプル吸い上げノズル、３は染色液、４はシ
ース液、５は染色槽、６はフローセル、７は対物レン
ズ、８はＴＶカメラ、９はサンプル分類のための識別演
算を行う特徴抽出回路、１０は画像表示用モニター、１
１は顕微鏡光源であるフラッシュランプ、１２はコンデ
ンサレンズ、１３は粒子検出用半導体レーザ、１４は粒
子検出器を示す。FIG. 1 is an outline of a flow type particle image analysis apparatus to which the standard solution according to the present invention is applied. 1 is a sample container, 2 is a sample suction nozzle, 3 is a dyeing solution, 4 is a sheath solution, 5 is a dyeing tank, 6 is a flow cell, 7 is an objective lens, 8 is a TV camera, 9 is an identification calculation for sample classification. Feature extraction circuit for performing, 10 is a monitor for image display, 1
1 is a flash lamp which is a light source of a microscope, 12 is a condenser lens, 13 is a semiconductor laser for particle detection, and 14 is a particle detector.
【００２６】サンプル容器１からサンプル吸い上げノズ
ル２によって分取されたサンプルは、染色槽５に吐出さ
れ、次に一定量吐出された染色液３により染色される。
染色されたサンプルはフローセル６の中心部分に測定サ
ンプルを供給するため、シース液４に包み込まれるよう
にしてフローセル内中央を安定に流れる。The sample collected from the sample container 1 by the sample suction nozzle 2 is discharged into the dyeing tank 5, and then dyed with the dyeing liquid 3 discharged in a fixed amount.
The stained sample supplies the measurement sample to the central portion of the flow cell 6, so that it is surrounded by the sheath liquid 4 and stably flows in the center of the flow cell.
【００２７】一方、顕微鏡光源であるフラッシュランプ
１１を出た光は顕微鏡光軸上を進み、コンデンサレンス
１２を通ってフローセル６内のサンプル上に集光され
る。このときＴＶカメラ８は静止サンプル画像を撮像し
たことになり特徴抽出回路９を経てサンプルの分類，存
在個数を求める。画像表示用モニター１０は画像処理結
果を表示したりサンプルの静止画像の表示を行う。On the other hand, the light emitted from the flash lamp 11 which is a microscope light source travels on the optical axis of the microscope, passes through the condenser 12, and is focused on the sample in the flow cell 6. At this time, the TV camera 8 has taken a still sample image, and the feature extraction circuit 9 is used to obtain the sample classification and the number of existing samples. The image display monitor 10 displays an image processing result and a still image of a sample.
【００２８】フラッシュランプ１１の発光タイミングは
粒子検出系の検出信号によって制御される。半導体レー
ザ１３は常時点灯しており、常にサンプル中の粒子が検
出領域を通過するのを粒子検出器１４で観測している。
粒子が通過し散乱光による粒子検出信号が所定の信号レ
ベル以上有れば、画像処理対象粒子と判断しサンプルが
ＴＶカメラの取り込み画像領域の所定の位置に達したと
き、フラッシュランプ１１が点灯する。The emission timing of the flash lamp 11 is controlled by the detection signal of the particle detection system. The semiconductor laser 13 is always on, and the particle detector 14 always observes that particles in the sample pass through the detection region.
If the particles pass and the particle detection signal due to the scattered light has a predetermined signal level or more, it is determined that the particles are image processing target particles, and when the sample reaches a predetermined position in the captured image area of the TV camera, the flash lamp 11 is turned on. .
【００２９】本発明による標準液を上記装置にて用いる
場合、サンプルと同様に扱うことによって使用される。
即ち、本標準液をサンプル容器１に分注することによっ
てサンプル同様に染色され、同じ流路を通ることにな
る。そして、サンプルより高濃度で均一な標準液を調製
をすることで、フローセル６の位置調整，粒子検出器１
４のタイミング調整，流速調整，フォーカス（対物レン
ズ７の位置）調整が容易になる。When the standard solution according to the present invention is used in the above apparatus, it is used by treating it like a sample.
That is, when the standard solution is dispensed into the sample container 1, the standard solution is dyed similarly to the sample and passes through the same channel. Then, by adjusting a uniform standard solution having a higher concentration than the sample, the position of the flow cell 6 is adjusted and the particle detector 1 is
4 timing adjustment, flow velocity adjustment, focus (position of the objective lens 7) adjustment becomes easy.
【００３０】また、サイズを選択したり粒子の染色性の
違いを組み合わせることで、粒子画像解析に用いられる
粒子の色・大きさなどの特徴パラメータのチェックが可
能となり、その結果特徴抽出回路９の分類アルゴリズム
のチェックが可能になる。Further, by selecting the size and combining the differences in the dyeability of the particles, it becomes possible to check the characteristic parameters such as the color and size of the particles used in the particle image analysis, and as a result, the characteristic extraction circuit 9 Allows checking of classification algorithms.
【００３１】さらに、粒子の形状を扁平で細長いものを
選択することで、サンプルの流れが安定ならば一定方向
（流れに沿った方向）であることが画像表示用モニター
１０で確認できフロー制御が容易になる。Furthermore, by selecting a flat and slender particle shape, if the sample flow is stable, it can be confirmed on the image display monitor 10 that the flow is in a fixed direction (a direction along the flow), and flow control can be performed. It will be easier.
【００３２】そのほか粒子の染色性をチェックすること
で、染色液の染色後の品質管理が安定かつ容易となる。In addition, by checking the dyeability of the particles, quality control after dyeing of the dyeing solution becomes stable and easy.
【００３３】以上のような各種メンテナンスが迅速かつ
容易に実施できる標準液の組成として、本装置に於ける
標準液を次のような構成とした。即ち、ポーラスポリマ
ー官能基導入樹脂１wt%、あるいは陽イオン交換樹脂１w
t%を標準液として使用し、サンプル同様染色され上記の
各種メンテナンスが可能であることを確認した。As the composition of the standard solution which enables various maintenances as described above to be carried out quickly and easily, the standard solution in this apparatus has the following constitution. That is, 1 wt% of a resin having a functional group introduced with a porous polymer, or 1 w of a cation exchange resin.
It was confirmed that t% was used as a standard solution and stained in the same manner as the sample, and that the above various maintenance was possible.
【００３４】[0034]
【発明の効果】本発明によれば、フロー式粒子画像解析
装置において、各種メンテナンスが迅速かつ容易に実施
でき装置の保守性能の向上がはかられたことにより、測
定データの信頼性の確保といった効果が期待できる。According to the present invention, in the flow type particle image analysis apparatus, various maintenances can be carried out quickly and easily, and the maintenance performance of the apparatus is improved, so that the reliability of measurement data is ensured. You can expect an effect.
【００３５】また、本標準液を用いることにより、メン
テナンス時の病原菌による感染の危険性がなくなり、長
期保存も可能となり取扱いの面に於いても効果を奏する
ものである。Further, by using this standard solution, there is no risk of infection by pathogenic bacteria during maintenance, long-term storage is possible, and it is effective in terms of handling.
【図１】フロー式粒子画像解析装置の全体構成図であ
る。FIG. 1 is an overall configuration diagram of a flow-type particle image analysis device.
１…サンプル容器、２…サンプル吸い上げノズル、３…
染色液、４…シース液、５…染色槽、６…フローセル、
７…対物レンズ、８…ＴＶカメラ、９…特徴抽出回路、
１０…画像表示用モニター、１１…フラッシュランプ、
１２…コンデンサレンズ、１３…粒子検出用半導体レー
ザ、１４…粒子検出器。1 ... Sample container, 2 ... Sample suction nozzle, 3 ...
Staining solution, 4 ... Sheath solution, 5 ... Staining tank, 6 ... Flow cell,
7 ... Objective lens, 8 ... TV camera, 9 ... Feature extraction circuit,
10 ... Monitor for image display, 11 ... Flash lamp,
12 ... Condenser lens, 13 ... Semiconductor laser for particle detection, 14 ... Particle detector.
Claims (5)
する粒子を光走査で検出し、さらに画像処理を行う装置
に用いる標準液に於いて、粒子の濃度，大きさ，形状が
任意に選択でき、染色液による染色が可能な人工的粒子
を用いることを特徴とするフロー式粒子画像解析装置用
標準液。1. A standard solution used in an apparatus for detecting particles floating in a continuously flowing measurement sample by optical scanning and further performing image processing, wherein the concentration, size, and shape of the particles are arbitrary. A standard solution for a flow-type particle image analyzer characterized by using artificial particles that can be selected and can be stained with a staining solution.
色性が一定，形は球状，扁平，棒状などで均一であるこ
とを特徴とするフロー式粒子画像解析装置用標準液。2. A standard liquid for a flow-type particle image analysis device, wherein the standard particles according to claim 1 have a uniform size and dyeability and are uniform in shape such as spherical, flat, and rod-shaped.
状，染色性の異なるものが数種類混在していることを特
徴とするフロー式粒子画像解析装置用標準液。3. A standard solution for a flow-type particle image analyzer, wherein the standard particles according to claim 1 are mixed with several kinds having different sizes, shapes and dyeing properties.
ーラスポリマー系，シリカ系，陽イオン，陰イオン等の
イオン交換樹脂であることを特徴とするフロー式粒子画
像解析装置用標準液。4. A standard solution for a flow type particle image analysis device, wherein the standard particle according to claim 2 or 3 is an ion exchange resin such as a porous polymer type, silica type, cation or anion. .
体積当たりの個数から粒子検出機構系の調整を、粒子の
大きさ，形，染色後の色からと画像処理系の特徴パラメ
ータの補正を、粒子の染色性から染色液の染色性及び品
質管理を、形状が横長扁平であることから測定サンプル
の流れの管理を安定に実施できることを特徴とするフロ
ー式粒子画像解析装置用標準液。5. The standard solution according to claim 1, wherein the particle detection mechanism system is adjusted based on the number of particles per unit volume, and the characteristic parameters of the image processing system are based on the size, shape, and color of dyed particles. The standard for the flow-type particle image analysis device is characterized by stable correction of the dyeability and quality control of the dyeing solution based on the dyeability of the particles, and stable control of the flow of the measurement sample due to the horizontally flat shape. liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24953692A JPH06102152A (en) | 1992-09-18 | 1992-09-18 | Standard solution for flow type particle picture analyzing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24953692A JPH06102152A (en) | 1992-09-18 | 1992-09-18 | Standard solution for flow type particle picture analyzing instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06102152A true JPH06102152A (en) | 1994-04-15 |
Family
ID=17194451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24953692A Pending JPH06102152A (en) | 1992-09-18 | 1992-09-18 | Standard solution for flow type particle picture analyzing instrument |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06102152A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0678742A2 (en) * | 1994-04-21 | 1995-10-25 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
| JPH08136439A (en) * | 1994-11-04 | 1996-05-31 | Toa Medical Electronics Co Ltd | Grain image analysis device |
| EP0774655A2 (en) | 1995-11-17 | 1997-05-21 | Toa Medical Electronics Co., Ltd. | Standard fluid for flow cytometer |
| JP2006162524A (en) * | 2004-12-09 | 2006-06-22 | Sysmex Corp | Standard solution for particle image analyzer |
| JP2007047154A (en) * | 2005-07-12 | 2007-02-22 | Sysmex Corp | Standard material for particle analyzer |
| JP2007114026A (en) * | 2005-10-19 | 2007-05-10 | Sysmex Corp | Standard substance for particle analyzer |
| JP2009115672A (en) * | 2007-11-08 | 2009-05-28 | Sony Corp | Optical measurement method and dispensing method for fine particle, and passage used for this optical measurement method and preparative method, and optical measurement device and flow cytometer |
| JP2009281753A (en) * | 2008-05-20 | 2009-12-03 | Hitachi Engineering & Services Co Ltd | Microorganism testing device and microorganism testing chip |
| US9658197B2 (en) | 2005-07-12 | 2017-05-23 | Sysmex Corporation | Standard material for a particle analyzer |
| WO2021132484A1 (en) * | 2019-12-27 | 2021-07-01 | シンクサイト株式会社 | Flow cytometer performance evaluation method and standard particle suspension |
-
1992
- 1992-09-18 JP JP24953692A patent/JPH06102152A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0678742A2 (en) * | 1994-04-21 | 1995-10-25 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
| EP0678742A3 (en) * | 1994-04-21 | 1996-01-31 | Hitachi Ltd | Monitoring method of stain solution for particle analysis and calibration method of particle analysis. |
| US5728582A (en) * | 1994-04-21 | 1998-03-17 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
| JPH08136439A (en) * | 1994-11-04 | 1996-05-31 | Toa Medical Electronics Co Ltd | Grain image analysis device |
| EP0774655A2 (en) | 1995-11-17 | 1997-05-21 | Toa Medical Electronics Co., Ltd. | Standard fluid for flow cytometer |
| US5888823A (en) * | 1995-11-17 | 1999-03-30 | Toa Medical Electronics Co., Ltd. | Standard fluid for flow cytometer |
| JP2006162524A (en) * | 2004-12-09 | 2006-06-22 | Sysmex Corp | Standard solution for particle image analyzer |
| JP4744132B2 (en) * | 2004-12-09 | 2011-08-10 | シスメックス株式会社 | Display value creation method for standard solution for particle image analyzer |
| JP2007047154A (en) * | 2005-07-12 | 2007-02-22 | Sysmex Corp | Standard material for particle analyzer |
| US9658197B2 (en) | 2005-07-12 | 2017-05-23 | Sysmex Corporation | Standard material for a particle analyzer |
| JP2007114026A (en) * | 2005-10-19 | 2007-05-10 | Sysmex Corp | Standard substance for particle analyzer |
| JP2009115672A (en) * | 2007-11-08 | 2009-05-28 | Sony Corp | Optical measurement method and dispensing method for fine particle, and passage used for this optical measurement method and preparative method, and optical measurement device and flow cytometer |
| JP2009281753A (en) * | 2008-05-20 | 2009-12-03 | Hitachi Engineering & Services Co Ltd | Microorganism testing device and microorganism testing chip |
| WO2021132484A1 (en) * | 2019-12-27 | 2021-07-01 | シンクサイト株式会社 | Flow cytometer performance evaluation method and standard particle suspension |
| EP4083603A4 (en) * | 2019-12-27 | 2024-04-03 | Thinkcyte Inc | Flow cytometer performance evaluation method and standard particle suspension |
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