JPH0564576A - Column type culturing device - Google Patents
Column type culturing deviceInfo
- Publication number
- JPH0564576A JPH0564576A JP22738091A JP22738091A JPH0564576A JP H0564576 A JPH0564576 A JP H0564576A JP 22738091 A JP22738091 A JP 22738091A JP 22738091 A JP22738091 A JP 22738091A JP H0564576 A JPH0564576 A JP H0564576A
- Authority
- JP
- Japan
- Prior art keywords
- column
- culture
- culture solution
- tank
- hollow fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は微生物、動物細胞等を培
養するためのホローファイバ型カラム、マトリックス型
セラミックカラム、多層不織布カラム等を用いたカラム
型培養装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a column type culturing apparatus using a hollow fiber type column, a matrix type ceramic column, a multilayer nonwoven fabric column, etc. for culturing microorganisms, animal cells and the like.
【０００２】[0002]
【従来の技術】微生物、動物細胞等の培養に用いるカラ
ム型培養装置は、ホローファイバカラムを例にとると、
例えば図２に示すように、カラム外郭１０内に半透膜や
濾過膜等でできた通液性のホローファイバ（中空糸）２
０を多数本配置したカラムａを主体とするものである。
外郭１０内のホローファイバ外空間に培養液を収容する
とともに、そこに培養対象物を配置する一方、カラムの
入口１１０及び出口１２０を培養液循環路３０に接続
し、該循環路に設けた培養液タンク４０中の液を、ガス
交換器５０にて酸素及び二酸化炭素等必要なガス成分を
含ませつつポンプ６０にてファイバ２０に潅流させる。
培養対象物への新鮮培養液の供給及びホローファイバ外
空間の老廃物の除去は、ホローファイバ２０中の培養液
の流れにより生じる圧力差及び濃度勾配による物質拡散
によるホローファイバ内外の液交換によって行われる。2. Description of the Related Art A column-type culture apparatus used for culturing microorganisms, animal cells, etc., using a hollow fiber column as an example,
For example, as shown in FIG. 2, a liquid-permeable hollow fiber (hollow fiber) 2 made of a semipermeable membrane, a filtration membrane, or the like inside the column shell 10.
The column a mainly has a large number of 0s arranged therein.
While the culture solution is stored in the hollow fiber outer space in the outer shell 10 and the culture object is placed therein, the inlet 110 and the outlet 120 of the column are connected to the culture solution circulation passage 30, and the culture provided in the circulation passage The liquid in the liquid tank 40 is perfused by the pump 60 into the fiber 20 while containing necessary gas components such as oxygen and carbon dioxide in the gas exchanger 50.
The supply of the fresh culture solution to the culture object and the removal of the waste products in the outer space of the hollow fiber are performed by the liquid exchange inside and outside the hollow fiber due to the substance diffusion due to the pressure difference and the concentration gradient generated by the flow of the culture solution in the hollow fiber 20. Be seen.
【０００３】[0003]
【発明が解決しようとする課題】しかしながら、前記培
養装置は、カラムａが培養液循環路３０にてタンク４
０、ガス交換器５０、ポンプ６０等に接続されているた
め、培養装置設置空間の利用効率が悪いという問題があ
った。また、前述のカラムの液入口１１０及び出口１２
０の断面積がカラム断面積に対し小さいため、外郭１０
内の各ホローファイバ２０に流れる培養液の量にばらつ
きが生じ、各培養対象物に供給される培養液が不均一と
なるという問題や、既述のとおりホローファイバ内外の
液交換がファイバ内の液流により生じる圧力差と物質拡
散によるため、液交換率が悪いという問題があった。ま
た、このような問題のあることから、カラムを大型化す
ると、培養対象物の生育が悪化した。However, in the culturing apparatus, the column a has the culture solution circulation passage 30 and the tank 4
0, the gas exchanger 50, the pump 60, etc., there is a problem that the utilization efficiency of the culture device installation space is poor. In addition, the liquid inlet 110 and the outlet 12 of the column described above.
Since the cross-sectional area of 0 is smaller than that of the column,
The problem that the amount of the culture solution flowing through each hollow fiber 20 inside the hollow fiber varies, and the culture solution supplied to each culture object becomes non-uniform, and the liquid exchange inside and outside the hollow fiber as described above There is a problem that the liquid exchange rate is poor because of the pressure difference caused by the liquid flow and the substance diffusion. In addition, because of such a problem, when the column was made larger, the growth of the culture object deteriorated.
【０００４】他の培養カラムを用いた装置でも同様の問
題があった。そこで本発明は、第１に、設置空間の利用
効率を従来より向上させ得るコンパクトな構造のカラム
型培養装置を提供することを目的とする。本発明は、第
２に、培養カラム内に流れる培養液の量を各部で均一に
するとともに、培養液の交換効率を高め、大型化しても
培養対象物を良好に生育させ得る前記装置を提供するこ
とを目的とする。The same problem occurs in devices using other culture columns. Therefore, a first object of the present invention is to provide a column-type culture device having a compact structure capable of improving the utilization efficiency of the installation space as compared with the conventional one. Secondly, the present invention provides the above-mentioned apparatus, which can make the amount of the culture solution flowing in the culture column uniform in each part, enhance the exchange efficiency of the culture solution, and can grow the culture object well even if the culture solution is increased in size. The purpose is to do.
【０００５】[0005]
【課題を解決するための手段】本発明は前記第１の目的
に従い、先ず、培養カラム、前記カラムを囲繞する培養
液タンク、及び前記カラム内培養液と前記タンク内培養
液を交換させる手段を備えたことを特徴とするカラム型
培養装置を提供するものである。前記培養液タンク中且
つ前記カラム外域に、必要に応じ、酸素ガス、二酸化炭
素ガス等のガス成分を供給するためのガス交換器を設置
することができ、また、必要に応じ、タンクの内又は外
に温度調節用ヒータを付設することもできる。According to the first object of the present invention, first, a culture column, a culture solution tank surrounding the column, and a means for exchanging the culture solution in the column with the culture solution in the tank are provided. The present invention provides a column-type culture device characterized by being provided. A gas exchanger for supplying gas components such as oxygen gas and carbon dioxide gas can be installed in the culture solution tank and in the outer region of the column, if necessary, and, if necessary, in the tank or It is also possible to attach a temperature adjusting heater to the outside.
【０００６】前記培養カラムとしては既に挙げたホロー
ファイバカラム、マトリックス型セラミックカラム、多
層不織布カラム等の各種カラム形状の培養担体が考えら
れる。前記カラムの外郭としては、物質透過性の有るも
のも、無いものも考えられる。As the culture column, various column-shaped culture carriers such as the hollow fiber column, the matrix type ceramic column, and the multi-layered nonwoven fabric column which have already been mentioned can be considered. As the outer shell of the column, there may be one having substance permeability and one having no substance permeability.
【０００７】さらに、前記カラム中にタンク外から細胞
等を播種できるポートを設けておいてもよい。前記カラ
ム内と前記カラム外のタンク内域とで培養液を交換する
手段としては種々考えられるが、比較的構造簡単で、培
養液のシールの上で好都合なものとして、前記カラムの
一端を閉じ、他端を開口した構成とし、該培養液交換手
段を、前記カラムの開口に接続した伸縮ベローズ型ポン
プと、前記カラムから前記カラム外のタンク内域へ液流
出を許すように該カラムの片側の端部に設けた逆止弁
と、前記タンク内域から前記カラム内へ液流入を許すよ
うに前記カラムのもう一つの端部に設けた逆止弁とを含
む構成とすることが考えられる。Further, a port may be provided in the column so that cells and the like can be seeded from the outside of the tank. Although various means are conceivable for exchanging the culture solution between the inside of the column and the inside of the tank outside the column, one of the columns is closed because the structure is relatively simple and convenient for sealing the culture solution. , An expansion bellows type pump having the other end opened and the culture medium exchange means connected to the opening of the column, and one side of the column so as to allow the liquid to flow out from the column into the tank internal region outside the column. It is conceivable to include a check valve provided at the end of the column and a check valve provided at the other end of the column so as to allow the liquid to flow into the column from the inner area of the tank. ..
【０００８】この場合、前記ベローズ型ポンプの伸縮に
応じて容易に液が循環できるように、必要に応じ、前記
タンク内をタンク外と均圧にする手段、例えば空気浄化
フィルタを接続した通気ポートを設けてもよい。さら
に、前記第２の目的を達成できるように、前記伸縮ベロ
ーズ型ポンプを、前記カラムの断面径と略同径にわたっ
て前記カラム開口に接続させることができる。In this case, a means for equalizing the inside of the tank with the outside of the tank as necessary, for example, a ventilation port to which an air purifying filter is connected so that the liquid can easily circulate according to the expansion and contraction of the bellows type pump. May be provided. Further, in order to achieve the second object, the expandable bellows type pump can be connected to the column opening over a diameter substantially equal to the cross-sectional diameter of the column.
【０００９】液交換手段の他の例として、液シール上好
都合なマグネティックポンプ、すなわち、モータ駆動さ
れる磁石体の磁力で、これとは液密に隔絶されたチャン
バ内のポンプ羽根を回すようにしたものにより、カラム
内とカラム外タンク内域に培養液を循環させることも考
えられる。As another example of the liquid exchanging means, a magnetic pump convenient for liquid sealing, that is, a magnetic force of a magnet driven by a motor is used to rotate a pump blade in a chamber which is liquid-tightly isolated from the magnetic pump. It is also conceivable to circulate the culture solution inside the column and inside the tank outside the column depending on what was done.
【００１０】[0010]
【作用】本発明カラム型培養装置によると、培養液交換
手段の作用で培養液は培養液タンクとカラム内で相互に
交換され、それによってカラム内の培養対象物へ新鮮培
養液が供給されるとともにカラム内の老廃物が除去さ
れ、培養対象物が生育していく。According to the column-type culture apparatus of the present invention, the culture medium is exchanged between the culture medium tank and the column by the action of the culture medium exchange means, thereby supplying the fresh culture medium to the culture object in the column. At the same time, waste products in the column are removed, and the culture object grows.
【００１１】前記培養カラムが、その一端が閉じられ、
他端が開口しており、前記液交換手段が、該カラムの開
口に接続した伸縮ベローズ型ポンプと、該カラムに設け
た前記逆止弁とを含むものであるときは、該ポンプの運
転により、タンク内培養液が一方の逆止弁から前記カラ
ム内へ流入し、該カラム内培養液が他方の逆止弁からカ
ラム外のタンク内域へ流出するように循環する。これに
よってカラム内培養液とタンク内培養液が相互交換され
る。The culture column is closed at one end,
When the other end is open and the liquid exchange means includes an expandable bellows type pump connected to the opening of the column and the check valve provided in the column, the tank is operated by operating the pump. The inner culture solution circulates from one check valve into the column, and the inner culture solution circulates from the other check valve into the tank inner region outside the column. As a result, the culture solution in the column and the culture solution in the tank are exchanged with each other.
【００１２】また、前記伸縮ベローズ型ポンプが前記カ
ラムの断面径とほぼ同径にわたって前記カラム開口に接
続されているときは、該ポンプの運転により培養液が、
カラム内の全域にほぼ均等に、且つ、加圧下に円滑に流
れる。Further, when the expandable bellows type pump is connected to the column opening over a diameter substantially equal to the cross-sectional diameter of the column, the culture medium is operated by the operation of the pump.
It flows almost uniformly over the entire area in the column and smoothly under pressure.
【００１３】[0013]
【実施例】以下、本発明の実施例を図１を参照して説明
する。図１は一実施例の構成を示す図である。この培養
装置はホローファイバカラム型培養装置であり、ホロー
ファイバカラムＡ及びこれを囲繞する培養液タンクＢを
含んでいる。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to FIG. FIG. 1 is a diagram showing the configuration of an embodiment. This culture apparatus is a hollow fiber column type culture apparatus, and includes a hollow fiber column A and a culture solution tank B surrounding the hollow fiber column A.
【００１４】カラムＡは円筒形のカラム外郭１と、その
内部に配置された多数のホローファイバ２とを備えてい
る。各ホローファイバ２は半透膜或いは濾過膜で形成さ
れており、ファイバ壁を通して培養液が行き来できるも
のである。ホローファイバ２は互いに平行に全体として
円柱状に配置され、その両端部は合成樹脂２１によって
カラム外郭１の内壁に固定されている。合成樹脂２１は
ホローファイバ２同士の隙間を液密に埋めているととも
にファイバと外郭内壁面との間隙も液密に埋めている。The column A comprises a cylindrical column shell 1 and a large number of hollow fibers 2 arranged inside it. Each hollow fiber 2 is formed of a semipermeable membrane or a filtration membrane, and the culture solution can pass through the fiber wall. The hollow fibers 2 are arranged in parallel with each other in a columnar shape as a whole, and both ends thereof are fixed to the inner wall of the column outer shell 1 by a synthetic resin 21. The synthetic resin 21 fills the gap between the hollow fibers 2 in a liquid-tight manner, and also fills the gap between the fiber and the outer wall surface in a liquid-tight manner.
【００１５】カラム外郭１はセラミックフィルタ製の円
筒体で、この外郭１の上端１１が閉じられることでカラ
ムＡの上端が閉じられており、外郭１の他端１２が開口
していることにより、カラムＡはその断面径のほぼ全体
にわたって開口している。カラムＡの下端開口１２には
カラム断面径と同径にわたって伸縮ベローズ型ポンプ３
が接続されており、このポンプ３は駆動装置４によって
伸縮駆動される。駆動装置４は図示しないモータにより
駆動されるクランク機構からなるもの、ピストンシリン
ダ装置からなるもの等何でもよい。The column shell 1 is a cylindrical body made of a ceramic filter, and by closing the upper end 11 of the shell 1, the upper end of the column A is closed, and the other end 12 of the shell 1 is open, The column A is open over almost the entire cross-sectional diameter. In the lower end opening 12 of the column A, a telescopic bellows type pump 3 having the same diameter as the column cross section is provided.
Are connected, and the pump 3 is driven to expand and contract by the drive unit 4. The drive device 4 may be anything such as a crank mechanism driven by a motor (not shown) or a piston cylinder device.
【００１６】カラムＡの上端部にはホローファイバ２内
の培養液がタンクＢ内へ流出することを許す逆止弁５が
接続され、カラムＡの下端部にはタンクＢ内の培養液が
ホローファイバ２内へ流入することを許す逆止弁６が接
続されている。タンクＢ内には上側の逆止弁５から流出
してきた培養液が下側の逆止弁６へ流入する過程で該培
養液に酸素ガスを供給するためのシリコンファイバ製の
ガス交換器７が設置されている。また、カラムＡにおけ
るホローファイバ外空間２０に細胞を播種するためのポ
ート８が培養液タンクＢからカラムＡへわたって設けて
ある。タンクＢの上端には該タンク内を外部と均圧にす
るためのポート９が設けてあり、これには空気浄化用の
フィルタ９１が接続してある。A check valve 5 which allows the culture solution in the hollow fiber 2 to flow into the tank B is connected to the upper end of the column A, and the culture solution in the tank B is hollow at the lower end of the column A. A check valve 6 is connected which allows the flow into the fiber 2. In the tank B, there is provided a silicon fiber gas exchanger 7 for supplying oxygen gas to the culture solution in the process in which the culture solution flowing out from the upper check valve 5 flows into the lower check valve 6. is set up. Further, a port 8 for seeding cells in the hollow fiber outer space 20 in the column A is provided from the culture solution tank B to the column A. A port 9 is provided at the upper end of the tank B for equalizing the pressure inside the tank with the outside, and a filter 91 for air purification is connected to this port 9.
【００１７】さらにタンクＢの外周には培養液の温度を
調節するためのシリコンラバーヒータＨが捲いてある。
以上説明した培養装置によると、培養液タンクＢ内の培
養液はヒータＨによって細胞培養に適度の温度に維持さ
れる。そして駆動装置４によるポンプ３の往復伸縮運動
によりタンクＢ内の培養液が下側の逆止弁６からカラム
Ａ内へ流れ込み、ホローファイバ２中を流れる。一方、
ホローファイバ２内の培養液はカラムＡから逆止弁５を
通ってタンクＢへ流出する。このようにタンクＢへ流出
した培養液はガス交換器７を通過して必要な酸素ガスを
供給され、再び下側の逆止弁６からカラムＡ内へ流入す
る。なお、タンクＢからカラムＡ内へ培養液が流れる
際、タンクＢ内とカラムＡ内に圧力差が生じることによ
り培養液の一部はカラムＡの外郭であるセラミックフィ
ルター１を通じてカラムＡ内に入り込み、また、カラム
Ａ内からタンクＢへ培養液が流出する際も同様にセラミ
ックフィルター１を通じてカラムＡからタンクＢへ一部
の液が流出する。Further, a silicon rubber heater H for adjusting the temperature of the culture solution is wound around the outer periphery of the tank B.
According to the culture apparatus described above, the culture solution in the culture solution tank B is maintained at a temperature suitable for cell culture by the heater H. Then, the reciprocating expansion / contraction motion of the pump 3 by the drive device 4 causes the culture solution in the tank B to flow into the column A from the check valve 6 on the lower side and to flow in the hollow fiber 2. on the other hand,
The culture solution in the hollow fiber 2 flows out from the column A through the check valve 5 into the tank B. The culture solution thus flowing out to the tank B passes through the gas exchanger 7 and is supplied with necessary oxygen gas, and again flows into the column A from the check valve 6 on the lower side. When the culture solution flows from the tank B into the column A, a pressure difference occurs between the tank B and the column A, so that a part of the culture solution enters the column A through the ceramic filter 1 which is the outer shell of the column A. Also, when the culture solution flows from the inside of the column A to the tank B, a part of the solution also flows from the column A to the tank B through the ceramic filter 1.
【００１８】このように培養液がホローファイバ２とカ
ラムＡ外のタンク内域に循環することにより、カラムＡ
内のホローファイバ外空間に撒かれた細胞が、主として
ホローファイバ２を通して新鮮培養液の供給を受け、且
つ、該空間２０における老廃物が主としてホローファイ
バ２を通してホローファイバ外へ運ばれていく。ポンプ
３の運転による前記培養液の循環動作中、タンクＢ内の
圧力はポート９を介して外部と均圧に保たれ、かくして
ポンプ３の往復運動による圧力変動を吸収するかたち
で、培養液の循環が円滑に行われる。By circulating the culture solution in the tank fiber region outside the hollow fiber 2 and the column A in this manner, the column A
The cells scattered in the hollow fiber outer space in the inside are supplied with the fresh culture liquid mainly through the hollow fiber 2, and the waste products in the space 20 are mainly transported through the hollow fiber 2 to the outside of the hollow fiber. During the circulation operation of the culture solution by the operation of the pump 3, the pressure in the tank B is kept equal to the outside through the port 9, thus absorbing the pressure fluctuation due to the reciprocating movement of the pump 3, The circulation is smooth.
【００１９】また、伸縮ベローズ型ポンプ３はカラムＡ
の断面径全体にわたってカラムＡに接続されているの
で、該ポンプ３が圧縮されるとき、ポンプ３内の培養液
がその圧力で各ホローファイバ２へ均等に流れ込み、且
つ、ファイバ２内の液流速度を速める。かくして各ホロ
ーファイバ２へほぼ均一に培養液が流れ、且つ、各ホロ
ーファイバ壁を通しての培養液交換が円滑に行われ、そ
の結果、ファイバ外空間２０における細胞がそれだけ均
一に、且つ、円滑に生育する。また、このようにホロー
ファイバ壁を通しての培養液交換が円滑に行われるた
め、装置全体を大型化しても細胞を良好に生育させるこ
とができる。The telescopic bellows type pump 3 is a column A.
Since it is connected to the column A over the entire cross-sectional diameter thereof, when the pump 3 is compressed, the culture solution in the pump 3 evenly flows into each hollow fiber 2 by its pressure, and the liquid flow in the fiber 2 Speed up. Thus, the culture solution flows into each hollow fiber 2 almost uniformly, and the culture solution is smoothly exchanged through each hollow fiber wall, and as a result, the cells in the outer space 20 of the fiber grow so uniformly and smoothly. To do. Further, since the culture medium can be smoothly exchanged through the hollow fiber wall in this manner, cells can be well grown even if the size of the entire apparatus is increased.
【００２０】さらに、前記実施例装置は、カラムＡの外
側を培養液タンクＢが囲繞する恰好に形成されているの
で、全体がコンパクトに形成されており、従って装置の
設置空間をそれだけ有効に利用することができ、単位空
間当たりの細胞培養効率を飛躍的に増進させることがで
きる。Furthermore, since the culture medium tank B surrounds the outer side of the column A in the apparatus of the embodiment, the whole apparatus is compactly formed, and therefore, the installation space of the apparatus can be effectively utilized. The cell culture efficiency per unit space can be dramatically improved.
【００２１】[0021]
【発明の効果】以上説明したように本発明によると、先
ず、設置空間の利用効率を従来より向上させ得るコンパ
クトな構造のカラム型培養装置を提供することができ
る。また、本発明によると、伸縮型ベローズポンプを利
用して、カラム内に流れる培養液の量を各部で均一にす
るとともに、培養液の交換効率を高め、大型化しても培
養対象物を良好に生育させ得る前記装置を提供すること
ができる。As described above, according to the present invention, firstly, it is possible to provide a column-type culture device having a compact structure capable of improving the utilization efficiency of the installation space as compared with the conventional case. Further, according to the present invention, by using the telescopic bellows pump, the amount of the culture solution flowing in the column is made uniform in each part, the exchange efficiency of the culture solution is increased, and the culture object is improved even if the size is increased. A device capable of growing can be provided.
【図１】本発明の一実施例の概略構成を示す図である。FIG. 1 is a diagram showing a schematic configuration of an embodiment of the present invention.
【図２】従来例の構成を示す図である。FIG. 2 is a diagram showing a configuration of a conventional example.
Ａ ホローファイバカラム Ｂ 培養液タンク １ カラム外郭 ２ ホローファイバ ２０ ファイバ外空間 １１ カラム閉塞端 １２ カラム開口 ３ ベローズ型ポンプ ４ 駆動装置 ５、６ 逆止弁 ７ ガス交換器 ８ 細胞播種ポート ９ 均圧用ポート ９１ フィルタ Ｈ ヒータ A hollow fiber column B culture solution tank 1 column shell 2 hollow fiber 20 fiber outer space 11 column closed end 12 column opening 3 bellows type pump 4 drive device 5, 6 check valve 7 gas exchanger 8 cell seeding port 9 equalization port 91 Filter H heater
Claims (4)
液タンク、及び前記カラム内培養液と前記タンク内培養
液を交換させる手段を備えたことを特徴とするカラム型
培養装置。1. A column-type culture device comprising: a culture column, a culture solution tank surrounding the column, and means for exchanging the culture solution in the column with the culture solution in the tank.
端が開口しており、前記液交換手段は、前記カラムの開
口に接続した伸縮ベローズ型ポンプと、前記カラムから
前記カラム外のタンク内域へ液流出を許すように該カラ
ムの片側端部に設けた逆止弁と、前記タンク内域から前
記カラム内へ液流入を許すように前記カラムのもう一つ
の端部に設けた逆止弁とを含んでいる請求項１記載のカ
ラム型培養装置。2. The column has one end closed and the other end open, and the liquid exchange means includes an expandable bellows type pump connected to the opening of the column, and a tank outside the column from the column. A check valve provided at one end of the column to allow liquid to flow out to the inner region, and a check valve provided at the other end of the column to allow liquid to flow from the tank inner region into the column. The column-type culture device according to claim 1, further comprising a stop valve.
の断面径と略同径にわたって前記カラム開口に接続され
ている請求項２記載のカラム型培養装置。3. The column-type culture device according to claim 2, wherein the expandable bellows-type pump is connected to the column opening over a diameter substantially equal to the cross-sectional diameter of the column.
である請求項１、２又は３記載のカラム型培養装置。4. The column-type culture device according to claim 1, 2 or 3, wherein the culture column is a hollow fiber column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22738091A JPH0564576A (en) | 1991-09-06 | 1991-09-06 | Column type culturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22738091A JPH0564576A (en) | 1991-09-06 | 1991-09-06 | Column type culturing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0564576A true JPH0564576A (en) | 1993-03-19 |
Family
ID=16859906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22738091A Withdrawn JPH0564576A (en) | 1991-09-06 | 1991-09-06 | Column type culturing device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0564576A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003089628A1 (en) * | 2002-04-19 | 2003-10-30 | Applied Cell Biotechnologies, Inc. | Cell culture method using porous membrane |
| KR101476518B1 (en) * | 2010-04-13 | 2014-12-24 | 가부시키가이샤 도모에 기쥬츠 겐큐쇼 | Valve element of butterfly valve |
| CN109966579A (en) * | 2019-05-24 | 2019-07-05 | 上海赛立维生物科技有限公司 | Biological reaction apparatus and bio-reaction system |
-
1991
- 1991-09-06 JP JP22738091A patent/JPH0564576A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003089628A1 (en) * | 2002-04-19 | 2003-10-30 | Applied Cell Biotechnologies, Inc. | Cell culture method using porous membrane |
| KR101476518B1 (en) * | 2010-04-13 | 2014-12-24 | 가부시키가이샤 도모에 기쥬츠 겐큐쇼 | Valve element of butterfly valve |
| CN109966579A (en) * | 2019-05-24 | 2019-07-05 | 上海赛立维生物科技有限公司 | Biological reaction apparatus and bio-reaction system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 19981203 |