JPH05336948A - Device for light culture - Google Patents
Device for light cultureInfo
- Publication number
- JPH05336948A JPH05336948A JP4149693A JP14969392A JPH05336948A JP H05336948 A JPH05336948 A JP H05336948A JP 4149693 A JP4149693 A JP 4149693A JP 14969392 A JP14969392 A JP 14969392A JP H05336948 A JPH05336948 A JP H05336948A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- light intensity
- depth
- liquid
- culture tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treating Waste Gases (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は光培養装置に関し、さら
に詳しくは太陽光を利用して効率よく藻類等による光合
成を行うことができる光培養装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a photoincubator, and more particularly to a photoincubator capable of efficiently performing photosynthesis by algae using sunlight.
【０００２】[0002]
【従来の技術】地球温暖化の要因の一つである大気中の
ＣＯ2 ガスの低減方法として、ＣＯ2を化学的または物
理的に吸着する方法、藻類の光合成能を利用する生物的
方法等が検討されている。藻類等を用いた生物的ＣＯ2
固定化技術開発の大きなテーマのひとつに太陽光を用い
て高効率的に藻類等による光合成を行うことができる大
型の光培養装置の開発がある。2. Description of the Related Art As a method of reducing CO 2 gas in the atmosphere, which is one of the causes of global warming, a method of chemically or physically adsorbing CO 2 , a biological method utilizing the photosynthetic ability of algae, etc. Is being considered. Biological CO 2 using algae
One of the major themes of immobilization technology development is the development of a large-scale photoincubator capable of highly efficiently performing photosynthesis by algae using sunlight.
【０００３】藻類の光合成においては、培養時間の経過
とともに藻類が増殖して菌体濃度が増大し、それにつれ
て光合成反応に必要な光の培養液中への光透過度が減少
するため、藻類の増殖速度が低下するという問題があ
る。この問題を解決する方法として、培養液中の菌体濃
度に応じて培養液の液深を変化させて透過光量を調整す
る方法、すなわち、菌体濃度が低い培養初期には培養槽
の液深を深くし、培養が進むにつれて液深を浅くする方
法が採用されている。しかしながら、従来、各培養槽毎
の液深の設定は、過去の経験に基づいて人為的に行って
いるため、特に太陽光を利用した場合には、日照条件等
の環境変化にきめこまかに対応することができず、高効
率で藻類等の光合成による培養を行うことができないと
いう問題があった。In photosynthesis of algae, the algae grows with the lapse of culture time to increase the bacterial cell concentration, and the light transmittance of the light necessary for the photosynthetic reaction into the culture solution decreases accordingly. There is a problem that the growth rate decreases. As a method to solve this problem, a method of adjusting the amount of transmitted light by changing the liquid depth of the culture solution according to the bacterial cell concentration in the culture solution, that is, the liquid depth of the culture tank at the early stage of culture when the bacterial cell concentration is low Is used to make the liquid deeper as the culture progresses. However, conventionally, the liquid depth of each culture tank is artificially set based on past experience, so when sunlight is used, it is possible to respond carefully to environmental changes such as sunshine conditions. Therefore, there is a problem that it is not possible to perform culture by photosynthesis of algae with high efficiency.
【０００４】[0004]
【発明が解決しようとする課題】本発明の目的は、前記
従来技術の問題を解決し、太陽光を利用して藻類等の光
合成による培養を行った場合でも、効率的に光合成によ
る培養を行うことができる光培養装置を提供することに
ある。SUMMARY OF THE INVENTION An object of the present invention is to solve the above-mentioned problems of the prior art and efficiently perform photosynthetic culture even when algae or the like are cultured by photosynthesis using sunlight. An object of the present invention is to provide an optical culture device capable of performing the above.
【０００５】[0005]
【課題を解決するための手段】本発明は、培養液の液深
の異なる複数の培養槽を備えた、太陽光を利用して菌体
の光合成を行う光培養装置において、前記各培養槽の培
養液上部と底部に照度計または光量子計、各培養槽内に
培養液の菌体濃度を測定する濃度測定器および培養液の
深さを測定する液深計および各培養槽上部に光強度の調
整可能な人工照明灯を設けるとともに、上記計測器の信
号から培養液の入射光強度に対する透過光強度の比が
０．６以上となる各培養槽の液深および人工照明灯の光
強度を算出する演算装置と、該演算装置の信号により各
培養槽の液深および人工照明灯の光強度を調節する手段
とを設けたことを特徴とする光培養装置に関する。Means for Solving the Problems The present invention provides a photoincubator for photosynthesizing bacterial cells using sunlight, which comprises a plurality of culture tanks having different culture liquid depths. An illuminometer or a photon meter at the top and bottom of the culture solution, a concentration meter to measure the bacterial cell concentration of the culture solution in each culture tank, a depth meter to measure the depth of the culture solution, and a light intensity indicator at the top of each culture tank. Adjustable artificial lighting is provided, and the liquid depth of each culture tank and the light intensity of the artificial lighting are calculated from the signal of the above measuring instrument so that the ratio of the transmitted light intensity to the incident light intensity of the culture solution is 0.6 or more. And a means for adjusting the liquid depth of each culture tank and the light intensity of an artificial illumination lamp according to the signal of the operation device.
【０００６】[0006]
【作用】藻類等の光合成反応において、栄養分、ＣＯ2
量、温度等の適性条件の範囲内で充分に存在している場
合には、光量が律速となり、図２に示すように、増殖速
度ｄｘ／ｄｔは、入射光量Ｉ0 と液深ｈ（すなわち透過
光量Ｉ）によって定まるため、入射光量の強度を減衰さ
せない環境を設定することにより高い増殖速度が得られ
る。培養液中の透過光強度の減衰は、Ｌａｍｂｅｒｔ−
ｂｅｅｒの式 Ｉ／Ｉ0 ＝ｅｘｐ（−ａｘｈ） （但し、Ｉ0 は入射光強度（ｌｘ）、Ｉは透過光強度
（ｌｘ）、ａは菌濃度と吸光度の関係ら予め求めた吸収
係数、ｈは液深（cm）およびｘは菌濃度（ｇ／ｌ）であ
る。）に従うため、入射光強度Ｉ0 、透過光強度Ｉおよ
び菌濃度ｘを測定することにより液深ｈが算出でき、菌
濃度および太陽光が変化した場合でも、Ｉ／Ｉ0 が一定
となるように液深ｈを自動的に制御することにより、安
定した高い増殖速度を得ることができる。[Function] In photosynthetic reaction of algae, nutrients, CO 2
When the amount of light is sufficient within the range of suitable conditions such as the amount and temperature, the light amount is rate-determining, and as shown in FIG. 2, the growth rate dx / dt is the incident light amount I 0 and the liquid depth h (that is, Since it is determined by the transmitted light amount I), a high growth rate can be obtained by setting an environment in which the intensity of the incident light amount is not attenuated. The attenuation of the transmitted light intensity in the culture solution is determined by the Lambert-
Beer's formula I / I 0 = exp (−axh) (where I 0 is the incident light intensity (lx), I is the transmitted light intensity (lx), and a is the absorption coefficient previously determined from the relationship between the bacterial concentration and the absorbance, Since h is the liquid depth (cm) and x is the bacterial concentration (g / l).), the liquid depth h can be calculated by measuring the incident light intensity I 0 , the transmitted light intensity I and the bacterial concentration x. Even when the bacterium concentration and sunlight change, a stable high growth rate can be obtained by automatically controlling the liquid depth h so that I / I 0 becomes constant.
【０００７】本発明において、Ｉ／Ｉ0 は藻類等の光合
成反応による菌体培養の点から、０．６以上、好ましく
は０．８以上に設定され、入射光強度Ｉ0 は、太陽光が
日照条件等により変化した場合でも、人工照明灯の光強
度の調整により一定に調節され、また培養槽の液深ｈ
は、Ｌａｍｂｅｒｔ−ｂｅｅｒの式から算出された上記
条件を満足する液深に調節される。In the present invention, I / I 0 is set to 0.6 or more, preferably 0.8 or more from the viewpoint of cell culture by a photosynthetic reaction of algae or the like, and the incident light intensity I 0 is set by the sunlight. Even if it changes due to sunshine conditions, etc., it is adjusted to a constant level by adjusting the light intensity of the artificial illumination lamp, and the liquid depth of the culture tank h
Is adjusted to a liquid depth that satisfies the above condition calculated from the Lambert-Beer equation.
【０００８】図１は、本発明の一実施例を示す３段池式
培養装置の説明図である。この装置は、それぞれ培養液
の液深の異なる培養槽１、２、３と、各培養槽の培養液
上部と底部にそれぞれ設けられた照度計４、５と、各培
養槽内に設けられた培養液の菌体濃度を測定する濃度測
定器６と、培養液の深さを測定する液深計７と、各培養
槽上部に設けられた光強度の調整可能な人工照明灯８
と、上記計測器４、５、６、７の信号から培養液の入射
光強度に対する透過光強度の比（Ｉ／Ｉ0 ）が０．６以
上となる各培養槽の液深ｈおよび入射光強度Ｉ0 を一定
とするのに必要な人工照明灯の光強度を算出する演算装
置１１と、該演算装置１１の液深調節信号１４により各
培養槽の液深を調節する移送ポンプ９と、培養槽３に設
けられた培養液抜出弁１６とから主としてなる。なお、
１０は太陽光、１５は光強度調節信号、１２は記録計で
ある。なお、菌濃度は、例えばオプティカルデンシティ
（ＯＤ）を用い、波長６００ｎｍの吸収量からあらかじ
め求めた検量線に基づいて換算することにより、または
濁度計により測定することができる。FIG. 1 is an explanatory view of a three-stage pond type culture apparatus showing one embodiment of the present invention. This apparatus was provided in each of the culture tanks 1, 2 and 3 having different culture liquid depths, the luminometers 4 and 5 respectively provided at the top and bottom of the culture solution of each culture tank, and each culture tank. Concentration measuring device 6 for measuring the bacterial cell concentration of the culture solution, liquid depth gauge 7 for measuring the depth of the culture solution, and artificial illumination lamp 8 provided at the upper part of each culture tank with adjustable light intensity.
And the liquid depth h of each culture tank and the incident light from which the ratio (I / I 0 ) of the transmitted light intensity to the incident light intensity of the culture liquid is 0.6 or more from the signals of the measuring instruments 4, 5, 6, and 7. An arithmetic unit 11 for calculating the light intensity of the artificial illumination lamp required to keep the intensity I 0 constant, and a transfer pump 9 for adjusting the liquid depth of each culture tank by the liquid depth adjustment signal 14 of the arithmetic unit 11. It mainly consists of a culture solution withdrawal valve 16 provided in the culture tank 3. In addition,
10 is sunlight, 15 is a light intensity adjustment signal, and 12 is a recorder. The bacterial concentration can be measured, for example, by using optical density (OD), by converting it based on a calibration curve previously obtained from the absorption amount at a wavelength of 600 nm, or by a turbidimeter.
【０００９】このような構成において、各培養槽１、
２、３は、光源として太陽光１０と人工照明灯８を併用
する。人工照明灯８は補助または調整用として適性光強
度の１０〜８０％を担うことができる。また各培養槽間
は、移送ポンプ９およびそれに接続された配管によって
結ばれており、培養液の順方向および逆方向の移送は弁
の開閉により任意に行うことができる。照度計４、５、
濃度測定器６および液深計７により計測された入射光強
度Ｉ0、透過光強度Ｉ、菌濃度ｘおよび液深ｈの信号１
３は演算器１１に送られ、演算処理されて培養条件の最
低条件が設定される。設定された信号１４、１５はそれ
ぞれ移送ポンプ９および人工照明灯８に送られる。In such a structure, each culture tank 1,
2 and 3 use the sunlight 10 and the artificial illumination lamp 8 together as a light source. The artificial lighting 8 can carry 10 to 80% of the appropriate light intensity for auxiliary or adjusting purposes. Further, the respective culture tanks are connected by a transfer pump 9 and a pipe connected thereto, and the forward and backward transfer of the culture solution can be arbitrarily performed by opening and closing a valve. Illuminometer 4, 5,
Signal 1 of incident light intensity I 0 , transmitted light intensity I, bacterium concentration x and liquid depth h measured by the concentration measuring device 6 and the liquid depth meter 7.
3 is sent to the calculator 11 and is subjected to calculation processing to set the minimum culture condition. The set signals 14 and 15 are sent to the transfer pump 9 and the artificial illumination lamp 8, respectively.
【００１０】例えば、Ｉ／Ｉ0 を０．７以上に設定した
場合、培養槽２のＩ2 ／Ｉ0 2 の値が０．５であれば、
まず人工照明灯８の光強度が光強度調節信号１５により
調節される。それでもＩ2 ／Ｉ0 2 が０．７にならない
場合には、移送ポンプ９を駆動して培養槽２の培養液を
培養槽１に逆送し、培養槽２の液深ｈ2 を下げてＩ2／
Ｉ0 2 値を０．７に維持させる。この操作によって培養
槽１のＩ1 ／Ｉ0 1 値が０．７を維持できなくなる場合
は、培養槽２の培養液を培養槽３に移送し、この操作と
同時に培養槽３の培養液抜出弁１６を開いて培養液の一
部を系外に排出する。以上の操作を各培養槽毎に行うこ
とにより、各槽のＩ／Ｉ0 を容易に設定値以上に維持す
ることができ、光エネルギーを効率よく利用して増殖速
度を向上させることができる。For example, when I / I 0 is set to 0.7 or more, if the value of I 2 / I 0 2 in the culture tank 2 is 0.5,
First, the light intensity of the artificial illumination lamp 8 is adjusted by the light intensity adjustment signal 15. If I 2 / I 0 2 still does not reach 0.7, the transfer pump 9 is driven to feed the culture solution in the culture tank 2 back to the culture tank 1, and the liquid depth h 2 in the culture tank 2 is lowered. I 2 /
The I 0 2 value is kept at 0.7. When the I 1 / I 0 1 value of the culture tank 1 cannot be maintained at 0.7 by this operation, the culture solution in the culture tank 2 is transferred to the culture tank 3, and at the same time as this operation, the culture solution is removed from the culture tank 3. The outlet valve 16 is opened to discharge a part of the culture solution out of the system. By performing the above operation for each culture tank, the I / I 0 of each tank can be easily maintained at the set value or more, and the light energy can be efficiently used to improve the growth rate.
【００１１】[0011]
【実施例】以下、本発明を実施例により詳しく説明す
る。 実施例１ 図１の３段池式培養装置を用い、各培養槽のＩ／Ｉ0 が
０．７以上となるように人工照射灯の光強度および液深
を自動的に調節して菌体（Ａｎａｂａｅｎａｃｙｌｉｎ
ｄｒｉｃａ）の培養を改変デトマー培地を培養液として
行った。各段の光強度範囲および滞留時間は、それぞれ
培養槽１で１〜３万ｌｘ、５日、培養槽２で２〜６万ｌ
ｘ、５日および培養槽３で２〜３万ｌｘ、４日とし、人
工照明灯８を補助光として調整して１４日間培養し、該
培養液を培養液抜出弁１６から抜き出した。培養温度は
２５〜３５℃とし、１０％のＣＯ2 濃度ガスを０．２ｖ
ｖｍで通気させた。１４日後に抜き出した菌体濃度は２
ｇ／ｌであった。このときの培養時間と菌体濃度の関係
を図３に実線で示した。EXAMPLES The present invention will be described in detail below with reference to examples. Example 1 Using the three-stage pond culture device of FIG. 1, the light intensity and the liquid depth of the artificial irradiation lamp were automatically adjusted so that the I / I 0 of each culture tank was 0.7 or more, and the bacterial cells were adjusted. (Anabaenacylin
culture was performed using a modified detomer medium as a culture medium. The light intensity range and residence time of each stage are 1 to 30,000 lx in the culture tank 1 and 5 days, and 20 to 60,000 l in the culture tank 2.
x, 5 days and 2 to 30,000 lx in the culture tank 3 and 4 days, the artificial illumination lamp 8 was adjusted as auxiliary light and culture was performed for 14 days, and the culture solution was extracted from the culture solution extraction valve 16. The culture temperature is 25 to 35 ° C., and 10% CO 2 concentration gas is 0.2 v.
Vented at vm. The cell concentration extracted after 14 days was 2
It was g / l. The relationship between the culture time and the bacterial cell concentration at this time is shown by the solid line in FIG.
【００１２】比較例１ 図１の装置を用いて光強度を５万ｌｘ、培養槽１、２、
３の液深をそれぞれ３０cm、２０cm、１０cmに固定した
以外は実施例１と同様の条件で菌体の培養を行った。１
４日後に抜き出した菌体濃度は１．５ｇ／ｌであった。
このときの培養時間と菌体濃度の関係を図３に破線で示
した。Comparative Example 1 Using the apparatus of FIG. 1, the light intensity was set to 50,000 lx, the culture tanks 1 and 2,
Bacterial cells were cultured under the same conditions as in Example 1 except that the liquid depths of 3 were fixed to 30 cm, 20 cm, and 10 cm, respectively. 1
The bacterial cell concentration extracted after 4 days was 1.5 g / l.
The relationship between the culture time and the bacterial cell concentration at this time is shown by a broken line in FIG.
【００１３】[0013]
【発明の効果】本発明の光培養装置によれば、太陽光を
利用して藻類等の光合成による培養を行った場合でも、
人工照明灯の光強度および培養液の液深を自動的に調節
してＩ／Ｉ0 を一定値以上に維持できるため、菌体の光
合成による培養を効率よく行うことができる。EFFECT OF THE INVENTION According to the photoincubator of the present invention, even when culturing algae or the like by photosynthesis using sunlight,
Since the I / I 0 can be maintained at a certain value or more by automatically adjusting the light intensity of the artificial illumination lamp and the liquid depth of the culture solution, it is possible to efficiently perform the culturing by microbial photosynthesis.
【図１】本発明の一実施例を示す３段池式培養装置の説
明図。FIG. 1 is an explanatory view of a three-stage pond culture device showing one embodiment of the present invention.
【図２】藻類等の光合成反応における入射光強度と増殖
速度の関係を示す図。FIG. 2 is a diagram showing the relationship between incident light intensity and growth rate in a photosynthetic reaction of algae or the like.
【図３】実施例１および比較例１の培養時間と菌体濃度
の関係を示す図。FIG. 3 is a graph showing the relationship between culture time and cell concentration in Example 1 and Comparative Example 1.
１、２、３…培養槽、４、５…照度計、６…濃度測定
器、７…液深計、８…人工照明灯、９…ポンプ、１０…
太陽光、１１…演算器、１２…記録計、１３…測定値信
号、１４…液深調節信号、１５…光強度調節信号。1, 2, 3 ... Culture tank, 4, 5 ... Illuminance meter, 6 ... Concentration measuring device, 7 ... Liquid depth meter, 8 ... Artificial illumination lamp, 9 ... Pump, 10 ...
Sunlight, 11 ... Computing device, 12 ... Recorder, 13 ... Measurement value signal, 14 ... Liquid depth adjustment signal, 15 ... Light intensity adjustment signal.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:89） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Office reference number FI technical display location C12R 1:89)
Claims (1)
えた、太陽光を利用して菌体の光合成を行う光培養装置
において、前記各培養槽の培養液上部と底部に照度計ま
たは光量子計、各培養槽内に培養液の菌体濃度を測定す
る濃度測定器および培養液の深さを測定する液深計およ
び各培養槽上部に光強度の調整可能な人工照明灯を設け
るとともに、上記計測器の信号から培養液の入射光強度
に対する透過光強度の比が０．６以上となる各培養槽の
液深および人工照明灯の光強度を算出する演算装置と、
該演算装置の信号により各培養槽の液深および人工照明
灯の光強度を調節する手段とを設けたことを特徴とする
光培養装置。1. A photoincubator for photosynthesizing bacterial cells using sunlight, comprising a plurality of culture vessels having different culture fluid depths, wherein an illuminometer is provided at the top and bottom of the culture fluid in each culture vessel. Or a photon meter, a concentration meter for measuring the bacterial cell concentration of the culture solution in each culture tank, a depth meter for measuring the depth of the culture solution, and an artificial illumination lamp with adjustable light intensity at the top of each culture tank Along with, a calculation device that calculates the liquid depth of each culture tank and the light intensity of the artificial illumination lamp in which the ratio of the transmitted light intensity to the incident light intensity of the culture liquid is 0.6 or more from the signal of the measuring instrument,
An optical culture device comprising means for adjusting the liquid depth of each culture tank and the light intensity of an artificial illumination lamp according to a signal from the arithmetic device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4149693A JP2716627B2 (en) | 1992-06-09 | 1992-06-09 | Light culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4149693A JP2716627B2 (en) | 1992-06-09 | 1992-06-09 | Light culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05336948A true JPH05336948A (en) | 1993-12-21 |
| JP2716627B2 JP2716627B2 (en) | 1998-02-18 |
Family
ID=15480751
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4149693A Expired - Fee Related JP2716627B2 (en) | 1992-06-09 | 1992-06-09 | Light culture device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2716627B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008153202A1 (en) * | 2007-06-14 | 2008-12-18 | Waseda University | Method of culturing photosynthetic microorganism using photosynthetic reactor allowed to float on water surface and photosynthetic reactor for producing hydrogen gas by photosynthetic microorganism |
| JP2015231349A (en) * | 2014-06-10 | 2015-12-24 | 株式会社日立製作所 | Culture system |
| CN105724231A (en) * | 2016-04-25 | 2016-07-06 | 崔学晨 | Artificial environment hydroponics production line |
| JP2019503817A (en) * | 2015-11-06 | 2019-02-14 | スエズ・グループ | Process to control air purification system |
| WO2024048588A1 (en) * | 2022-08-31 | 2024-03-07 | インテグリカルチャー株式会社 | Cell culture system, cultured meat, and culture supernatant |
-
1992
- 1992-06-09 JP JP4149693A patent/JP2716627B2/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008153202A1 (en) * | 2007-06-14 | 2008-12-18 | Waseda University | Method of culturing photosynthetic microorganism using photosynthetic reactor allowed to float on water surface and photosynthetic reactor for producing hydrogen gas by photosynthetic microorganism |
| JP2015231349A (en) * | 2014-06-10 | 2015-12-24 | 株式会社日立製作所 | Culture system |
| JP2019503817A (en) * | 2015-11-06 | 2019-02-14 | スエズ・グループ | Process to control air purification system |
| US10807037B2 (en) | 2015-11-06 | 2020-10-20 | Suez Groupe | Process for controlling air purification systems |
| CN105724231A (en) * | 2016-04-25 | 2016-07-06 | 崔学晨 | Artificial environment hydroponics production line |
| WO2024048588A1 (en) * | 2022-08-31 | 2024-03-07 | インテグリカルチャー株式会社 | Cell culture system, cultured meat, and culture supernatant |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2716627B2 (en) | 1998-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Qiang et al. | Productivity and photosynthetic efficiency of Spirulina platensis as affected by light intensity, algal density and rate of mixing in a flat plate photobioreactor | |
| Hu et al. | A flat inclined modular photobioreactor for outdoor mass cultivation of photoautotrophs | |
| Torzillo et al. | A two‐plane tubular photobioreactor for outdoor culture of Spirulina | |
| CN100374539C (en) | Photobioreactor and process for biomass production and mitigation of pollutants in flue gases | |
| JP3266619B2 (en) | Biomass production equipment | |
| US5541056A (en) | Method of control of microorganism growth process | |
| GB1495709A (en) | Method and apparatus for growing plant cells | |
| NO986176L (en) | Method for regulating Haematococcus spp. Growth process | |
| JP2743316B2 (en) | Tubular photobioreactor | |
| JP2716627B2 (en) | Light culture device | |
| CN203187688U (en) | Algae cultivation box | |
| Bruyant et al. | An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes | |
| Rinanti et al. | Integrated vertical photobioreactor system for carbon dioxide removal using phototrophic microalgae | |
| Heaven et al. | Light attenuation parameters for waste stabilisation ponds | |
| JPH07155167A (en) | Culture device for fine alga | |
| US5232855A (en) | Apparatus for use in a axenic mass culture | |
| JPH08322553A (en) | Cultivation of fine algae and equipment therefor | |
| JPH11346760A (en) | Cultivation of microalga | |
| CN108676710B (en) | Method and device for simulating influence of blue algae decomposition on generation and release of greenhouse gases | |
| Chen et al. | Microalgae continuous and semi-continuous cultures | |
| Hadiningrat et al. | The utilization of microalgae as an agent for converting CO2 to O2 in a photosynthesis reactor to mitigate CO2 emissions | |
| CN2543962Y (en) | Reactor | |
| CN212315710U (en) | Water treatment equipment | |
| JPH01211484A (en) | Method for germ-free mass culture and apparatus therefor | |
| JPH07184631A (en) | Culture apparatus for photosynthetic microorganism and culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 19971007 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20081107 Year of fee payment: 11 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20091107 Year of fee payment: 12 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20091107 Year of fee payment: 12 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20101107 Year of fee payment: 13 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20101107 Year of fee payment: 13 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20111107 Year of fee payment: 14 |
|
| LAPS | Cancellation because of no payment of annual fees |