JPH05328963A - Method for culturing algae and plant cell and light source device for culturing - Google Patents
Method for culturing algae and plant cell and light source device for culturingInfo
- Publication number
- JPH05328963A JPH05328963A JP4165315A JP16531592A JPH05328963A JP H05328963 A JPH05328963 A JP H05328963A JP 4165315 A JP4165315 A JP 4165315A JP 16531592 A JP16531592 A JP 16531592A JP H05328963 A JPH05328963 A JP H05328963A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- culturing
- culture solution
- fluorescent lamp
- light
- Prior art date
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- Granted
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、例えばクロレラやスピ
ルリナ等の藻類、及び植物細胞の培養方法に関するもの
である。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for culturing algae such as chlorella and spirulina, and plant cells.
【０００２】[0002]
【従来の技術】従来より、人工光源を利用した藻類や植
物細胞の主な培養方法としては、次の４つが知られてい
る。第１には図２に示すように、透明な筒型の培養槽１
の外部に設置したランプ２により、培養槽１内の培養液
に光を照射する方法がある。第２には図３に示すよう
に、扁平な薄型の培養槽３の片面或は両面の外部に設置
したランプ４により、培養槽３内の培養液に光を照射す
る方法がある。2. Description of the Related Art Conventionally, the following four methods are known as main culture methods for algae and plant cells using an artificial light source. First, as shown in FIG. 2, a transparent cylindrical culture tank 1
There is a method of irradiating the culture solution in the culture tank 1 with light by using a lamp 2 installed outside. Second, as shown in FIG. 3, there is a method of irradiating the culture solution in the culture tank 3 with light by a lamp 4 installed on one or both sides of the flat and thin culture tank 3.
【０００３】第３には図４に示すように、筒形の培養槽
５に設けた内筒５ａに収納したランプ６により、培養槽
５の内側から該培養槽５内の培養液に光を照射する方法
がある。第４には図５及び図６に示すように、培養槽７
の内壁に配設した光ファイバ８へキセノンランプ等の光
源９からの光を導光し、この導光された光を光ファイバ
８の周面から培養槽７の内部に放射させて、培養槽７内
の培養液に光を照射する方法がある。Thirdly, as shown in FIG. 4, a lamp 6 housed in an inner cylinder 5a provided in a cylindrical culture tank 5 emits light from the inside of the culture tank 5 to the culture solution in the culture tank 5. There is a method of irradiation. Fourth, as shown in FIGS. 5 and 6, the culture tank 7
The light from the light source 9 such as a xenon lamp is guided to the optical fiber 8 disposed on the inner wall of the cell, and the guided light is radiated from the peripheral surface of the optical fiber 8 into the culture tank 7, and There is a method of irradiating the culture solution in 7 with light.
【０００４】人工光源を選択するのに当たっては、光源
のランプ効率、寿命、価格、光の波長が細胞増殖に適し
ているか否か、調光が可能か否かといった点がポイント
となり、総合的に評価すると蛍光ランプが最も優れてい
る。このため、上述した４つの方法の中では、図２乃至
図４に示した第１乃至第３の方法が、光源に蛍光ランプ
を使用できる点で好適な方法であると言える。In selecting an artificial light source, the lamp efficiency of the light source, the life, the price, whether the wavelength of light is suitable for cell proliferation, and whether dimming is possible are important points. When evaluated, fluorescent lamps are the best. Therefore, among the above-mentioned four methods, the first to third methods shown in FIGS. 2 to 4 can be said to be preferable methods because a fluorescent lamp can be used as a light source.
【０００５】[0005]
【発明が解決しようとする課題】しかしながら、図２及
び図３に示した第１及び第２の方法では、ランプ２，４
からの光が照射される培養槽１，３の表面積に制限があ
り、また、培養槽１，３の表面において光の反射が生じ
るので、ランプ２，４から発光される光の全光量のうち
一部しか培養槽１，３内に導入することができない。こ
のため、ランプ２，４の光量に比べて培養液の照度を上
げることができず、培養槽１，３内の藻類等の増殖速度
が遅くなる等、培養を効率的に行うことができなくなる
不具合があった。However, in the first and second methods shown in FIGS. 2 and 3, the lamps 2, 4 are
Since there is a limit to the surface area of the culture tanks 1 and 3 that are irradiated with the light from, and the light is reflected on the surfaces of the culture tanks 1 and 3, of the total amount of light emitted from the lamps 2 and 4. Only a part can be introduced into the culture tanks 1 and 3. Therefore, the illuminance of the culture solution cannot be increased as compared with the light amount of the lamps 2 and 4, and the growth rate of algae or the like in the culture tanks 1 and 3 becomes slow, so that the culture cannot be efficiently performed. There was a problem.
【０００６】また、図４に示した第３の方法では、内筒
５ａの表面における光の反射は若干あるにせよ、ランプ
６から発光される光のほぼ全光量を培養槽５内に導入す
ることができる。しかし、内筒５ａを形成する分だけ培
養槽５の容量が減ってしまい、例えば、スケールアップ
のため光源を多数設置しなければならない場合には、そ
の分培養液の量を減らさなければならず、やはり培養を
効率的に行うことができなくなる不具合があった。Further, in the third method shown in FIG. 4, although there is some reflection of light on the surface of the inner cylinder 5a, almost the total amount of light emitted from the lamp 6 is introduced into the culture tank 5. be able to. However, the capacity of the culture tank 5 is reduced by the amount of forming the inner cylinder 5a. For example, when a large number of light sources must be installed for scale-up, the amount of culture solution must be reduced accordingly. However, there was a problem that the culture could not be performed efficiently.
【０００７】さらに、蛍光ランプは交流点灯方式である
ため、図４に示した培養槽５の内筒５ａに蛍光ランプを
収納すると、蛍光ランプを点灯させるのに必要な高周波
電流で培養槽５内の培養液に誘導電流が生じて、培養液
と接する培養装置の金属部分からアースに電流がリーク
し、このリークした高周波電流が培養細胞に影響を及ぼ
して、細胞の増殖速度を鈍化させる不具合もあった。Further, since the fluorescent lamp is an AC lighting system, if the fluorescent lamp is housed in the inner cylinder 5a of the culture tank 5 shown in FIG. 4, the high-frequency current necessary for lighting the fluorescent lamp causes the inside of the culture tank 5 to rise. Induced current is generated in the culture solution, and the current leaks from the metal part of the culture device contacting the culture solution to the ground, and the leaked high-frequency current affects the cultured cells, which slows down the cell growth rate. there were.
【０００８】本発明は上述の問題を解決するためになさ
れたもので、藻類や植物細胞の培養に蛍光ランプを光源
として用いる場合に、電流のリークを防ぎつつ高い導光
効率を維持し、増殖速度を鈍化させることなく効率的な
培養を行うことができる藻類及び植物細胞の培養方法を
提供することを目的とする。The present invention has been made to solve the above problems, and when a fluorescent lamp is used as a light source for culturing algae or plant cells, it prevents leakage of electric current while maintaining high light guiding efficiency and multiplication. It is an object of the present invention to provide a method for culturing algae and plant cells, which enables efficient culturing without slowing down the speed.
【０００９】[0009]
【課題を解決するための手段】上記目的を達成するため
に本発明は、培養槽内の培養液に蛍光ランプの光を照射
して前記培養液内の藻類及び植物細胞の培養を行う培養
方法であって、前記蛍光ランプの少なくとも一部を前記
培養液に浸漬し、前記蛍光ランプを直流電流により点灯
させるようにしたことを特徴とする。また、本発明は、
培養液内の藻類及び植物細胞の培養を行うための培養用
光源装置であって、少なくとも一部が前記培養液に浸漬
される直流点灯型の蛍光ランプと、前記蛍光ランプを点
灯させるための直流安定器とを備えることを特徴とす
る。In order to achieve the above object, the present invention provides a culture method in which a culture solution in a culture tank is irradiated with light from a fluorescent lamp to culture algae and plant cells in the culture solution. In addition, at least a part of the fluorescent lamp is immersed in the culture solution, and the fluorescent lamp is turned on by a direct current. Further, the present invention is
A culture light source device for culturing algae and plant cells in a culture solution, a direct current lighting fluorescent lamp at least a part of which is immersed in the culture solution, and a direct current for lighting the fluorescent lamp. And a ballast.
【００１０】[0010]
【実施例】以下、本発明の実施例を図面に基づき説明す
る。図１は本発明の一実施例による培養装置の構成説明
図である。Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram illustrating the configuration of a culture device according to an embodiment of the present invention.
【００１１】図１において１０は、アクリル等の透明な
素材により形成された培養槽であり、その内部には、藍
藻（Spirulina Platensis 、以下、スピルリナと称す
る）を浸漬した培養液が充填されている。培養槽１０の
上端１１には排液管２２が接続されており、この排液管
２２は培養液の環流管２１の上端に連通されている。一
方、培養槽１０の下端１２側壁には給液管２３が接続さ
れており、この給液管２３はマグネットポンプ２４を介
して環流管２１の下端に連通されている。このため、マ
グネットポンプ２４を作動させると、培養液が培養槽１
０内から排液管２２、環流管２１、マグネットポンプ２
４、及び給液管２３を経由して再び培養槽１０内に環流
される。尚、環流管２１の上端からは排気管２５が上方
に延出している。In FIG. 1, reference numeral 10 denotes a culture tank formed of a transparent material such as acryl, and the inside thereof is filled with a culture solution in which cyanobacteria (Spirulina Platensis, hereinafter referred to as "spirulina") is immersed. .. A drainage pipe 22 is connected to the upper end 11 of the culture tank 10, and the drainage pipe 22 is communicated with the upper end of a reflux pipe 21 for the culture solution. On the other hand, a liquid supply pipe 23 is connected to the side wall of the lower end 12 of the culture tank 10, and the liquid supply pipe 23 is connected to the lower end of the reflux pipe 21 via a magnet pump 24. For this reason, when the magnet pump 24 is operated, the culture solution is removed from the culture tank 1.
From 0 to drain pipe 22, return pipe 21, magnet pump 2
4, and is recirculated into the culture tank 10 again via the liquid supply pipe 23. An exhaust pipe 25 extends upward from the upper end of the reflux pipe 21.
【００１２】また、培養槽１０の下端１２には多孔質の
散気板１３が設けられており、エアフィルタ３４を介し
てマスフローコントローラ３３が接続されている。この
マスフローコントローラ３３には、エアコンプレッサ３
１と炭酸ガスボンベ３２とが接続されており、マスフロ
ーコントローラ３３からエアフィルタ３４及び散気板１
３を介して培養槽１０内へ、炭酸ガスを混合した空気が
無菌空気として供給される。A porous diffuser plate 13 is provided at the lower end 12 of the culture tank 10, and a mass flow controller 33 is connected via an air filter 34. This mass flow controller 33 includes an air compressor 3
1 and a carbon dioxide gas cylinder 32 are connected to each other, and the mass flow controller 33 to the air filter 34 and the air diffuser 1
The air mixed with carbon dioxide gas is supplied as sterile air into the culture tank 10 via 3.
【００１３】さらに、培養槽１０の内部には、湿度調節
機４１に接続されたステンレス管４２が配設されてお
り、湿度調節機４１でステンレス管４２を冷却或は加熱
することにより、培養槽１０内の培養液の温度を任意の
温度に調節できるようにしている。Further, inside the culture tank 10, a stainless tube 42 connected to the humidity controller 41 is provided. By cooling or heating the stainless tube 42 by the humidity controller 41, the culture tank 10 is cooled. The temperature of the culture medium in 10 can be adjusted to any temperature.
【００１４】さて、培養槽１０の上端１１からその内部
へは、片口金タイプで直流点灯型の蛍光ランプ５０が挿
通されて、培養槽１０内の培養液に浸漬されており、培
養槽１０の上端１１からその外部に露出する蛍光ランプ
５０の口金部分にはコネクタ６２が接続されている。こ
のコネクタ６２は配線コード６１の先端に設けられてお
り、蛍光ランプ５０には、不図示の商用電源に接続され
た直流安定器６０からの直流電流が、配線コード６１及
びコネクタ６２を介して供給される。A single-base type direct current lighting fluorescent lamp 50 is inserted from the upper end 11 of the culture tank 10 into the inside thereof, and is immersed in the culture solution in the culture tank 10. A connector 62 is connected to the base portion of the fluorescent lamp 50 exposed from the upper end 11 to the outside. This connector 62 is provided at the tip of the wiring cord 61, and the fluorescent lamp 50 is supplied with a direct current from a DC ballast 60 connected to a commercial power source (not shown) via the wiring cord 61 and the connector 62. To be done.
【００１５】このような構成による本実施例の培養装置
でスピルリナの培養を行う際には、湿度調節機４１で培
養液の温度を調節し、マグネットポンプ２４を作動させ
て環流管２１内の培養液を給液管２３から培養槽１０内
へ吐出させる。また、これと同時に、培養槽１０の下端
１１に設けられた散気板１３から培養液内へ、炭酸ガス
が混合された無菌空気を圧送する。When culturing Spirulina in the culturing apparatus of this embodiment having such a structure, the temperature of the culturing solution is adjusted by the humidity controller 41, and the magnet pump 24 is actuated to cultivate in the reflux pipe 21. The liquid is discharged from the liquid supply pipe 23 into the culture tank 10. At the same time, aseptic air mixed with carbon dioxide is pressure-fed into the culture solution from the air diffuser plate 13 provided at the lower end 11 of the culture tank 10.
【００１６】これにより、培養槽１０内の培養液を、給
液管２３から吐出される培養液と、これと交差して散気
板１３から圧送される無菌空気とにより激しく撹拌させ
る。さらに、無菌空気の圧送によるエアリフト効果によ
って、培養槽１０内の培養液を排液管２２に排出させ、
排液管２２に排出された培養液を、環流管２１、マグネ
ットポンプ２４、及び給液管２３を介して再び培養槽１
０内へと環流させる。As a result, the culture liquid in the culture tank 10 is vigorously agitated by the culture liquid discharged from the liquid supply pipe 23 and the sterile air that is pressure-fed from the diffuser plate 13 to intersect with the culture liquid. Further, the culture solution in the culture tank 10 is discharged to the drain pipe 22 by the air lift effect by the aseptic air pressure feeding,
The culture fluid discharged to the drainage pipe 22 is again passed through the reflux pipe 21, the magnet pump 24, and the liquid supply pipe 23 to the culture tank 1 again.
Circulate into 0.
【００１７】そして、この状態で、直流安定器６０から
の直流電流で蛍光ランプ５０を発光させ、培養槽１０内
の培養液に光を照射させることにより、培養液中のスピ
ルリナを増殖させる。Then, in this state, the fluorescent lamp 50 is caused to emit light by the direct current from the direct current ballast 60, and the culture solution in the culture tank 10 is irradiated with light to grow spirulina in the culture solution.
【００１８】このように、本実施例の培養装置では、培
養液に光を照射する蛍光ランプ５０を培養槽１０内の培
養液に浸漬したので、培養槽１０の容量をそれほど減ら
さずに培養液へ光を効率よく照射することができる。ま
た、蛍光ランプ５０を、直流安定器６０からの直流電流
で点灯する直流点灯型としたので、培養液に誘導電流が
生じて培養液と接する培養装置の金属部分からアースに
電流がリークするのを防止することができる。よって、
リーク電流によるスピルリナの培養への影響をなくして
増殖速度の鈍化を防ぎ、培養液中のスピルリナの培養を
効率よく行うことができる。As described above, in the culture apparatus of this embodiment, the fluorescent lamp 50 for irradiating the culture solution with light is immersed in the culture solution in the culture tank 10. Therefore, the capacity of the culture tank 10 is not reduced so much. The light can be efficiently emitted. Further, since the fluorescent lamp 50 is a direct current lighting type that is turned on by the direct current from the direct current stabilizer 60, an induced current is generated in the culture solution, and the current leaks from the metal part of the culture apparatus in contact with the culture solution to the ground. Can be prevented. Therefore,
It is possible to eliminate the influence of leakage current on the culture of Spirulina, prevent the growth rate from slowing down, and efficiently culture Spirulina in the culture solution.
【００１９】尚、本実施例では、培養液を培養槽１０、
排液管２２、環流管２１、マグネットポンプ２４、給液
管２３、そして培養槽１０へと環流させて、培養槽１０
内の培養液を撹拌するものとしたが、培養槽１０内の培
養液を撹拌する手段はこれに限らず、培養槽１０内に撹
拌棒等を配設してこの撹拌棒により培養液を撹拌するよ
うにしてもよく、培養液を撹拌するための構成を省略し
てもよい。In this embodiment, the culture solution is added to the culture tank 10,
The drainage pipe 22, the recirculation pipe 21, the magnet pump 24, the liquid supply pipe 23, and the culture tank 10 are circulated, and the culture tank 10
Although the culture solution in the culture tank is stirred, the means for stirring the culture solution in the culture tank 10 is not limited to this, and a stirring rod or the like is provided in the culture tank 10 to stir the culture solution with the stirring rod. Alternatively, the configuration for stirring the culture solution may be omitted.
【００２０】次に、上記構成による本実施例の培養装置
と、該培養装置における直流安定器６０の代わりにイン
バータ安定器を用いた構成の培養装置とを用いて行っ
た、スピルリナの培養実験の結果について説明する。Next, a spirulina culturing experiment was carried out using the culturing apparatus of the present embodiment having the above-mentioned configuration and the culturing apparatus having an inverter ballast instead of the DC ballast 60 in the culturing apparatus. The results will be described.
【００２１】尚、本実験における各種条件は次のとおり
である。 培養槽１０ ： アクリル製、内径１１ｃ
ｍ、長さ９０ｃｍ 環流管２１ ： アクリル製、内径４ｃｍ、
長さ１５０ｃｍ 排液管２２ ： アクリル製、内径４ｃｍ、
長さ１５ｃｍ 給液管２３ ： ステンレス製、内径１ｃ
ｍ、長さ４ｃｍ ステンレス管４２ ： １ｃｍφ マグネットポンプ２４ ： 定格出力１５Ｗ 蛍光ランプ５０ ： ９６Ｗタイプ×２灯 使用藻株 ： スピルリナ 培養温度 ： ３５℃ 照度 ： ４０ｋｌｕｘ（培養槽１０
の中心部） 無菌空気 ： 炭酸ガス濃度０．４％の空
気 散気板１３からの無菌空気圧送量 ： ３．００リット
ル／ｍｉｎ． 使用培地 ： ＳＯＴ培地Various conditions in this experiment are as follows. Incubator 10: Acrylic, inner diameter 11c
m, length 90 cm reflux tube 21: acrylic, inner diameter 4 cm,
Length 150 cm Drainage pipe 22: Acrylic, inner diameter 4 cm,
Length 15 cm Liquid supply pipe 23: Stainless steel, inner diameter 1c
m, length 4 cm Stainless tube 42: 1 cmφ Magnet pump 24: Rated output 15 W Fluorescent lamp 50: 96 W type x 2 lamps Algae strain used: Spirulina Culture temperature: 35 ° C Illuminance: 40 klux (culture tank 10
Aseptic air: air with a carbon dioxide concentration of 0.4% Aseptic air pressure from the diffuser plate 13: 3.00 liters / min. Medium used: SOT medium
【００２２】また、ＳＯＴ培地１００ミリリットル中の
組成を表１に示す。The composition in 100 ml of SOT medium is shown in Table 1.
【００２３】[0023]
【表１】 [Table 1]
【００２４】さらに、Ａ5 金属混液１００ミリリットル
中の組成を表２に示す。Further, Table 2 shows the composition in 100 ml of the A 5 metal mixed solution.
【００２５】[0025]
【表２】 [Table 2]
【００２６】以上の条件の下、本実験では、本実施例の
培養装置と、該培養装置における直流安定器６０の代わ
りにインバータ安定器を用いた構成の培養装置とでそれ
ぞれ３日間スピルリナの培養を行った。サンプリング
は、毎日培養液を１０ミリリットルずつ採取してポアサ
イズ１μｍのメンブレンフィルタで吸引濾過後乾燥し
て、スピルリナの乾燥重量を計測した。その際の、それ
ぞれの培養装置における培養液と接する金属部分からア
ースに流れる電流の測定結果を表３に示す。Under the above conditions, in the present experiment, culturing of Spirulina was carried out for 3 days by using the culturing apparatus of this example and the culturing apparatus having an inverter ballast instead of the DC ballast 60 in the culturing apparatus. I went. For sampling, 10 ml of the culture solution was sampled every day, suction-filtered with a membrane filter having a pore size of 1 μm, and then dried, and the dry weight of spirulina was measured. Table 3 shows the measurement results of the current flowing from the metal part in contact with the culture solution in each culture device to the ground at that time.
【００２７】[0027]
【表３】 [Table 3]
【００２８】この表３に示すように、蛍光ランプの安定
器として直流安定器６０を用いた本実施例の培養装置で
は、インバータ安定器を用いた培養装置に比べて電流の
リークが明らかに改善されていることが分かる。そし
て、それぞれの培養装置によるスピルリナの培養成績を
示す表４に示すように、本実施例の培養装置によるスピ
ルリナの生産量は、インバータ安定器を用いた培養装置
に比べて１６．２ｇ多く、スピルリナの増殖効率を向上
できることが分かった。As shown in Table 3, in the culturing apparatus of this embodiment using the DC ballast 60 as the ballast of the fluorescent lamp, the current leakage is obviously improved as compared with the culturing apparatus using the inverter ballast. You can see that it is done. Then, as shown in Table 4 showing the culture results of Spirulina by each culture apparatus, the production amount of Spirulina by the culture apparatus of the present example is 16.2 g more than that of the culture apparatus using the inverter ballast, and Spirulina It was found that the growth efficiency of the
【００２９】[0029]
【表４】 [Table 4]
【００３０】[0030]
【発明の効果】以上説明したように本発明によれば、培
養槽内の培養液に蛍光ランプの光を照射して前記培養液
内の藻類及び植物細胞の培養を行うのに際して、蛍光ラ
ンプの少なくとも一部を前記培養液に浸漬し、この蛍光
ランプを直流電流により点灯させるようにしたので、培
養液を介しての電流のリークを防ぎつつ高い導光効率を
維持し、藻類及び植物細胞の増殖速度を鈍化させること
なく効率的な培養を行うことができる。As described above, according to the present invention, when a culture solution in a culture tank is irradiated with light of a fluorescent lamp to culture algae and plant cells in the culture solution, At least a part was immersed in the culture solution, and the fluorescent lamp was lit by direct current, so that high light guiding efficiency was maintained while preventing leakage of current through the culture solution, and algae and plant cells Efficient culture can be performed without slowing down the growth rate.
【図１】本発明の一実施例による培養装置の構成説明図
である。FIG. 1 is a structural explanatory view of a culture device according to an embodiment of the present invention.
【図２】従来の培養装置の一例を示す構成説明図であ
る。FIG. 2 is a structural explanatory view showing an example of a conventional culture device.
【図３】従来の培養装置の他の例を示す構成説明図であ
る。FIG. 3 is a configuration explanatory view showing another example of a conventional culture device.
【図４】従来の培養装置の他の例を示す構成説明図であ
る。FIG. 4 is a structural explanatory view showing another example of a conventional culture device.
【図５】従来の培養装置の他の例を示す構成説明図であ
る。FIG. 5 is a structural explanatory view showing another example of a conventional culture device.
【図６】従来の培養装置の他の例を示す構成説明図であ
る。FIG. 6 is a structural explanatory view showing another example of a conventional culture device.
１０ 培養槽 ５０ 蛍光ランプ ６０ 直流安定器 10 Culture tank 50 Fluorescent lamp 60 DC stabilizer
Claims (2)
射して前記培養液内の藻類及び植物細胞の培養を行う培
養方法であって、 前記蛍光ランプの少なくとも一部を前記培養液に浸漬
し、 前記蛍光ランプを直流電流により点灯させるようにし
た、 ことを特徴とする藻類及び植物細胞の培養方法。1. A culture method for culturing algae and plant cells in the culture solution by irradiating the culture solution in the culture tank with light of a fluorescent lamp, wherein at least a part of the fluorescent lamp is used in the culture solution. The method for culturing algae and plant cells, characterized in that the fluorescent lamp is soaked in a lamp and the fluorescent lamp is turned on by a direct current.
うための培養用光源装置であって、 少なくとも一部が前記培養液に浸漬される直流点灯型の
蛍光ランプと、 前記蛍光ランプを点灯させるための直流安定器と、 を備えることを特徴とする培養用光源装置。2. A culture light source device for culturing algae and plant cells in a culture solution, comprising a direct current lighting type fluorescent lamp at least a part of which is immersed in the culture solution, and the fluorescent lamp. A light source device for culture, comprising: a DC ballast for lighting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4165315A JP2916041B2 (en) | 1992-06-01 | 1992-06-01 | Algae and plant cell culture method and culture light source device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4165315A JP2916041B2 (en) | 1992-06-01 | 1992-06-01 | Algae and plant cell culture method and culture light source device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05328963A true JPH05328963A (en) | 1993-12-14 |
| JP2916041B2 JP2916041B2 (en) | 1999-07-05 |
Family
ID=15810000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4165315A Expired - Fee Related JP2916041B2 (en) | 1992-06-01 | 1992-06-01 | Algae and plant cell culture method and culture light source device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2916041B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002262858A (en) * | 2001-03-06 | 2002-09-17 | Tokai Sangyo Kk | Method for cultivating blue-breen algae |
| JP2006014627A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| JP2006014628A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| JP2014204698A (en) * | 2013-04-15 | 2014-10-30 | 清水建設株式会社 | Air supply system, and microorganism culture apparatus including the same |
| JP2019004807A (en) * | 2017-06-27 | 2019-01-17 | 有限会社サンおきなわ | Methods for culturing microalgae |
-
1992
- 1992-06-01 JP JP4165315A patent/JP2916041B2/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002262858A (en) * | 2001-03-06 | 2002-09-17 | Tokai Sangyo Kk | Method for cultivating blue-breen algae |
| JP2006014627A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| JP2006014628A (en) * | 2004-06-30 | 2006-01-19 | Koito Ind Ltd | Culture apparatus |
| JP4519542B2 (en) * | 2004-06-30 | 2010-08-04 | 小糸工業株式会社 | Incubator |
| JP2014204698A (en) * | 2013-04-15 | 2014-10-30 | 清水建設株式会社 | Air supply system, and microorganism culture apparatus including the same |
| JP2019004807A (en) * | 2017-06-27 | 2019-01-17 | 有限会社サンおきなわ | Methods for culturing microalgae |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2916041B2 (en) | 1999-07-05 |
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