JPH05219897A - Galactooligosaccharide-containing feed - Google Patents
Galactooligosaccharide-containing feedInfo
- Publication number
- JPH05219897A JPH05219897A JP4026840A JP2684092A JPH05219897A JP H05219897 A JPH05219897 A JP H05219897A JP 4026840 A JP4026840 A JP 4026840A JP 2684092 A JP2684092 A JP 2684092A JP H05219897 A JPH05219897 A JP H05219897A
- Authority
- JP
- Japan
- Prior art keywords
- lactose
- feed
- galactooligosaccharide
- livestock
- galacto
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、ガラクトオリゴ糖を特
定量配合した家畜用飼料に関するものであって、該飼料
は家畜の下痢、軟便の防止および体重の増加するのに効
果のあるものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a feed for livestock containing a specific amount of galactooligosaccharide, which feed is effective in preventing diarrhea and loose stools of livestock and increasing body weight. ..
【0002】[0002]
【従来の技術】最近、ヒトの腸内におけるビフイズス菌
の増殖因子として知られているガラクトオリゴ糖を主成
分とする糖類を含有させた家畜用飼料が提案されてい
る。(特開昭60−34134号)。また、ガラクトオ
リゴ糖を家畜用飼料に利用する試みもなされている(特
公平2−57902)。しかし、この発明において使用
されるガラクトオリゴ糖の添加量は0.1及至2重量%
配合と添加量が高く、かならずしも実際の家畜飼育者に
とっては満足のいくものではない。2. Description of the Related Art Recently, there has been proposed a livestock feed containing a saccharide containing galactooligosaccharide as a main component, which is known as a growth factor of bifidobacteria in the human intestine. (JP-A-60-34134). In addition, attempts have been made to utilize galacto-oligosaccharides as feed for livestock (Japanese Patent Publication No. 2-57902). However, the amount of galactooligosaccharide used in this invention is 0.1 to 2% by weight.
The composition and the amount added are high, and it is not always satisfactory for actual livestock breeders.
【0003】この他にもガラクトオリゴ糖は現在乳糖に
微生物由来の酵素を作用させて作る転移ガラクトオリゴ
糖(特公昭58−20266号、特開昭60−2518
96号等)や大豆の豆乳ホエーからの大豆オリゴ等(特
開昭62−155082号)やビート糖中のラフィノー
スを利用する抽出法(特開昭62−126951号)な
どで作られている。これらの方法で作られたものは目的
とするガラクトオリゴ糖の純度が50%前後と低く、ま
たこれらはいずれも液状の製品であるため(75%程度
の濃度)、ガラクトオリゴ糖分は更に低く、また液状の
ために利用しにくい難点もある。そのためにクロマト分
画などにより高純度のものを取り出して粉末化してい
る。一般に従来のガラクトオリゴ糖の発表されている糖
組成は第1表のようになっている。In addition to these, galactooligosaccharides are currently transferred galactooligosaccharides produced by the action of a microorganism-derived enzyme on lactose (JP-B-58-20266, JP-A-60-2518).
96) and soybean oligo from soybean whey (Japanese Patent Laid-Open No. 62-155082) and an extraction method using raffinose in beet sugar (Japanese Patent Laid-Open No. 62-126951). Products produced by these methods have a target galacto-oligosaccharide with a low purity of around 50%, and since these are liquid products (concentration of about 75%), the galacto-oligosaccharide content is even lower and liquid. Because of this, there are some drawbacks that are difficult to use. Therefore, high-purity substances are taken out and powdered by chromatographic fractionation. The published sugar composition of conventional galactooligosaccharides is generally as shown in Table 1.
【0004】[0004]
【表1】 [Table 1]
【0005】これらの問題点を改善する方法として、乳
糖に微生物を作用させて作る方法(特開平2−7289
0)が提案されている。また酵素反応によらない高純度
のガラクトオリゴ糖製造法として、酸を用いる製造方法
が開示されているが(特開平3−197490)、得ら
れたガラクトオリゴ糖の活性やその使用方法に関する具
体的な検討はなされていない。As a method for improving these problems, a method of making lactose act on a microorganism (Japanese Patent Laid-Open No. 7289/1990)
0) is proposed. Further, as a high-purity galacto-oligosaccharide production method that does not rely on an enzymatic reaction, a production method using an acid is disclosed (Japanese Patent Laid-Open No. 3-197490), but a specific study on the activity of the obtained galacto-oligosaccharide and a method for using the same. Not done.
【0006】[0006]
【発明が解決しようとする課題】本発明の課題は、家畜
の下痢、軟便の防止および体重の増加に効果がある実用
的に優れた家畜用飼料を提供することにある。SUMMARY OF THE INVENTION An object of the present invention is to provide a practically excellent animal feed which is effective in preventing diarrhea and loose stools of livestock and increasing body weight.
【0007】[0007]
【課題を解決するための手段】本発明者らは、微生物
や、酸を用いる高純度のガラクトオリゴ糖製造法(特開
平2−72890、3−197490)で得られたガラ
クトオリゴ糖の新たな用途を開発する目的でその活性に
ついて検討している過程において、家畜の主要な腸内細
菌であるバチルス属あるいはバクデロイド属等に属する
有用細菌が増殖し、その結果、ウエルシュ菌、クロスト
ドウム菌等の有害細菌の生育が抑制される可能性のある
ことを認め、このような推定に基づいてガラクトオリゴ
糖類の特定量を配合した飼料を子牛や子豚にような家畜
に給与したところ、従来知られていたよりはるかに少な
い添加量で、子牛や子豚の育成において屡々みられる下
痢軟便が有効に阻止され、かつ体重の増加も著しいこと
の知見を得た。また、さらに驚くべきことに、母豚の飼
料に配合して給与することにより、子豚の下痢発生率が
低下し、体重増加効果もあることを見いだし本発明を完
成した。[Means for Solving the Problems] The inventors of the present invention have proposed a new use of a galactooligosaccharide obtained by a method for producing a high-purity galactooligosaccharide using a microorganism or an acid (JP-A-2-72890, 3-197490). In the process of studying its activity for the purpose of development, useful bacteria belonging to the genus Bacillus or Bacteroides, which are the main enterobacteria of livestock, proliferate, and as a result, toxic bacteria such as Clostridium perfringens Recognizing that there is a possibility that growth may be suppressed, when a feed containing a specific amount of galacto-oligosaccharides was fed to livestock such as calves and piglets based on such an estimation, it was much better than previously known. It was found that diarrhea loose stool, which is often observed in raising calves and piglets, can be effectively prevented with a small addition amount, and that the weight gain is remarkable. Further, surprisingly, the present invention was completed by discovering that the diarrhea incidence rate of piglets is reduced and the body weight increasing effect is also obtained by adding and feeding it to the feed of sows.
【0008】すなわち、本発明は、上記知見に基づいて
なされたものであって、家畜の下痢軟便の防止および体
重増加に顕著な効果を奏するガラクトオリゴ糖を配合し
た家畜用飼料を提供することを目的とする。以下本発明
を詳しく説明する。[0008] That is, the present invention has been made based on the above findings, and an object of the present invention is to provide a livestock feed containing a galacto-oligosaccharide that has a remarkable effect on preventing loose diarrhea of livestock and increasing body weight. And The present invention will be described in detail below.
【0009】本発明の特徴は、ガラクトオリゴ糖を0.
01及至0.1重量%配合して成る家畜用飼料にある。
ここで用いるガラクトオリゴ糖とは、乳糖にガラクトー
スが1分子結合したガラクトシルラクトースと、乳糖に
ガラクトースが2分子結合したジガラクトシルラククト
ースを主に意味する。本発明で活性成分として用いるガ
ラクトオリゴ糖類が、下記方法により調製し得る。A feature of the present invention is that galacto-oligosaccharides are
01 to 0.1% by weight of the feed for livestock.
The galacto-oligosaccharide used herein mainly means galactosyl lactose in which one molecule of galactose is bound to lactose and digalactosyl lactose in which two molecules of galactose are bound to lactose. The galactooligosaccharide used as an active ingredient in the present invention can be prepared by the following method.
【0010】微生物を用いるガラクトオリゴ糖の製造
は、特開平2−72890に開示された方法により行な
うことができる。すなわち、ロドトルラ属またはステリ
グマトマイセス属またはシロバシディウム属に属する微
生物を乳糖または乳糖含有物に作用せしめ生成するガラ
ジュトオリゴ糖を採取することにより行なわれる。The production of galacto-oligosaccharides using microorganisms can be carried out by the method disclosed in JP-A-2-72890. That is, it is carried out by collecting a galactu-oligosaccharide produced by allowing a microorganism belonging to the genus Rhodotorula, the genus Sterigumatomyces or the genus Sirobacidium to act on lactose or a substance containing lactose.
【0011】酸を用いるガラクトオリゴ糖の製造は、特
開平3−197490に開示された方法により行なうこ
とができる。先ず原料の乳糖は特に限定されず広く従来
から知られているものが使用できる。例えば市販品でも
よく、製造上、混酸を水溶液として実施するものでは、
α−乳糖、β−乳糖、平衡乳糖何れでも使用できるが、
混酸を粉体のまま実施する場合はα−乳糖を使用するの
がよい。ガラクトースは市販品を使用することができ
る。乳糖とガラクトースを混合して使用する場合、特に
その比率はこだわらないが、通常その混合比率は9:1
〜5:5好ましくは8:2〜7:3(重量比)がよい。The production of galactooligosaccharide using an acid can be carried out by the method disclosed in JP-A-3-197490. First, the raw material lactose is not particularly limited, and widely known ones can be used. For example, a commercially available product may be used, and in the case of carrying out the mixed acid as an aqueous solution in production,
Although any of α-lactose, β-lactose and balanced lactose can be used,
When the mixed acid is used as a powder, α-lactose is preferably used. Galactose can use a commercial item. When lactose and galactose are used as a mixture, the ratio is not particularly limited, but the mixing ratio is usually 9: 1.
˜5: 5, preferably 8: 2 to 7: 3 (weight ratio).
【0012】次に触媒たる酸としては塩酸、硝酸、リン
酸などの無機酸が使用できるが、硫酸は脱水作用があ
り、あまり好ましくない。酸の量としては極小量でよく
重量比として乳糖またはこれとガラクトースの総量(乾
燥重量)当り0.1%以下0.01%前後まででよい。
酸は原料の乳糖或いは乳糖とガラクトースの混合物に充
分よく混合するため、1%程度に薄めて、これを霧状に
して原料とよく混合して乾燥して脱水するか、または原
料を水溶液としこれに所定量の酸を加えて混合しスプレ
ードライ等の方法で脱水するのがよい。次に脱水した原
料混合物を通常100〜200℃の温度に0.5〜3時
間好ましくは1〜2時間保存することで、重縮合を起こ
させ、ガラクトオリゴ糖に変換させる。この場合温度は
100℃未満では目的のガクトオリゴ糖ができにくく、
200℃より高くなると乳糖の融点に近くなり溶融した
り、着色したりするため望ましくない。加熱時間も0.
5時間未満では収率が特に高くならず、3時間より長く
なると着色やこげが生じて望ましくない。Next, as the catalyst acid, inorganic acids such as hydrochloric acid, nitric acid and phosphoric acid can be used, but sulfuric acid has a dehydrating action and is not so preferable. The amount of the acid may be a very small amount, and the weight ratio may be from 0.1% to 0.01% based on the total amount (dry weight) of lactose or galactose.
Since the acid mixes well with the raw material lactose or a mixture of lactose and galactose, it is diluted to about 1%, atomized and mixed well with the raw material and dried, or the raw material is made into an aqueous solution. It is preferable that a predetermined amount of acid is added to and mixed with and dehydrated by a method such as spray drying. Next, the dehydrated raw material mixture is usually stored at a temperature of 100 to 200 ° C. for 0.5 to 3 hours, preferably 1 to 2 hours to cause polycondensation to be converted into a galactooligosaccharide. In this case, if the temperature is less than 100 ° C., it is difficult to produce the desired gactooligosaccharide,
When the temperature is higher than 200 ° C, the melting point of lactose becomes close to the melting point and the coloring occurs, which is not desirable. The heating time is also 0.
If it is less than 5 hours, the yield is not particularly high, and if it is longer than 3 hours, coloring or burning occurs, which is not desirable.
【0013】このようにしてできたガラクトオリゴ糖は
水に溶かし中和後常法に従って脱色、脱塩し、溶液の状
態で使用することができる。この場合ガラクトオリゴ糖
の濃度は溶解度いっぱいまで使用可能であり通常60%
程度が好ましい。温度、湿度にもよるがガラクトオリゴ
糖の濃度が薄い溶液の場合には、保存中のガラクトオリ
ゴ糖溶液の中にカビが発生する場合があるので、カビが
発生することを防止する為にプロピオン酸又はプロピオ
ン酸ソーダなどの有機酸又はその塩を適当量、通常約
0.01%及至0.03%添加すれば良い。溶液状態で
本製品を使用する場合は、飼料への添加時の操作性が良
く本製品を均一に混合することができる。又操作中に本
製品飛び散らない為に作業環境が良好に保てる等の利点
がある。粉末製品として使用する場合には更に濃縮、ス
プレードライして粉末製品とする。The galactooligosaccharide thus obtained can be used in the form of a solution by dissolving it in water and neutralizing it, followed by decolorization and desalting according to a conventional method. In this case, the concentration of galacto-oligosaccharide can be used up to the solubility and is usually 60%.
A degree is preferable. Depending on the temperature and humidity, in the case of a solution with a low concentration of galacto-oligosaccharide, mold may occur in the galacto-oligosaccharide solution during storage.Therefore, propionic acid or propionic acid should be added to prevent mold formation. An organic acid such as sodium propionate or a salt thereof may be added in an appropriate amount, usually about 0.01% to 0.03%. When this product is used in solution, it has good operability when added to feed and can be mixed uniformly. Moreover, there is an advantage that the working environment can be kept good because this product does not scatter during operation. When it is used as a powder product, it is further concentrated and spray-dried to give a powder product.
【0014】これらのガラクトオリゴ糖を、飼料組成物
に0.01乃至0.1重量%配合することにより家畜の
下痢、軟便の防止および体重の増加に効果がある家畜用
飼料を調製できる。配合量が0.01重量%未満では効
果が不十分であり、0.1重量%より多いと下痢が増加
する等効果が減少する。By blending these galactooligosaccharides in a feed composition in an amount of 0.01 to 0.1% by weight, it is possible to prepare a feed for livestock which is effective in preventing diarrhea and loose stools of livestock and increasing body weight. If the blending amount is less than 0.01% by weight, the effect is insufficient, and if it is more than 0.1% by weight, the effect such as diarrhea is decreased.
【0015】本発明のガラクトオリゴ糖を配合する家畜
用飼料に配合される他の飼料原料は、通常用いられる家
畜用飼料原料を用いればよく、例えば玄米、トウモロコ
シ等の穀類、大豆粕や油脂類、無機塩類、アミノ酸類、
ビタミン類、人工乳等が用いられる。これらを家畜の種
類や成長段階にあわせ、常法により適宜配合して飼料組
成物として用いられる。As the other feed raw material to be added to the livestock feed containing the galactooligosaccharide of the present invention, a commonly used livestock feed raw material may be used, for example, grains such as brown rice and corn, soybean meal and oils and fats, Inorganic salts, amino acids,
Vitamins, artificial milk, etc. are used. These are used as a feed composition by appropriately blending them according to the type and growth stage of livestock by a conventional method.
【0016】[0016]
合成例1 微生物を用いるガラクトオリゴ糖の調製 特開平2−72890実施例1に従ってガラクトオリゴ
糖を含む培養液を調製し、菌体を分離後、イオン交換樹
脂処理により精製してガラクトオリゴ糖を調製した。Synthesis Example 1 Preparation of galacto-oligosaccharide using microorganisms A culture solution containing galacto-oligosaccharide was prepared according to JP-A-2-72890 Example 1, and the microbial cells were separated and purified by an ion exchange resin treatment to prepare a galacto-oligosaccharide.
【0017】合成例2 酸を用いるガラクトオリゴ糖の
調製 特開平3−197490に記載の方法に従って調製し
た。すなわち、市販品のα−乳糖100gに1.0%塩
酸溶液5mlを加圧空気を用いてスプレーし、更にミキサ
ーにて均一に混合後、アルミバットに入れ、乾燥器で8
0℃で1時間予備乾燥し、ついで180℃で3時間加熱
焙焼した。ガラクトオリゴ糖であることの確認、分子量
分布、糖組成などは培養液及び水溶液と酸加水分解した
ものをHPLCで検定して測定した。このようにして得
られた重合物の糖組成は以下の通りであった。(%はい
ずれも重量%、以下同じ)Synthesis Example 2 Preparation of galactooligosaccharide using acid It was prepared according to the method described in JP-A-3-197490. That is, 100 g of a commercially available α-lactose was sprayed with 5 ml of a 1.0% hydrochloric acid solution using pressurized air, and the mixture was evenly mixed with a mixer, placed in an aluminum vat, and dried with a dryer.
It was pre-dried at 0 ° C. for 1 hour and then heated and roasted at 180 ° C. for 3 hours. Confirmation of galactooligosaccharide, molecular weight distribution, sugar composition, etc. were measured by assaying the culture solution and aqueous solution with acid-hydrolyzed by HPLC. The sugar composition of the polymer thus obtained was as follows. (% Is% by weight, the same applies below)
【0018】[0018]
【表2】 [Table 2]
【0019】実施例1 ガラクトオリゴ糖配合飼料の調
製と子牛飼育試験 合成例で調製したオリゴ糖粉末を、下記の配合の飼料
に、ガラクトオリゴ糖を0.005重量%、0.01重
量%、0.09重量%および0.3重量%含有するよう
に添加して離乳期子牛用配合飼料それぞれ調製した。 全脂粉乳 50重量部 脱脂粉乳 20重量部 ビタミンおよびミネラル 3重量部Example 1 Preparation of Feed Containing Galactooligosaccharide and Calf Rearing Test The oligosaccharide powder prepared in the synthesis example was added to the feed having the following composition in an amount of 0.005% by weight, 0.01% by weight, and 0% by weight of galactooligosaccharide. 0.09% by weight and 0.3% by weight were added to prepare mixed feeds for weaning calves. Whole milk powder 50 parts by weight Skim milk powder 20 parts by weight Vitamins and minerals 3 parts by weight
【0020】子牛の飼育試験:上述のようにして調製し
たガラクトオリゴ糖含量の異なる各飼料を下記方法に従
って子牛に給与して、下痢、軟便の発生率と体重増加率
を測定した。なお、比較として合成例のガラクトオリゴ
糖に代えて、フラクトオリゴ糖や酵素を作用させて調製
した(特公平2−57902、実施例1の方法で調製し
た、以下同様)ガラクトオリゴ糖を0.01重量%と
0.1重量%含有させた飼料、ならびに対照としてオリ
ゴ糖類を含有しない飼料を同様に給与した場合について
も測定した。 試験方法:生後1週間のホルスタイン種雄牛の20頭か
ら成る各試験区に、上記各飼料を給与して3週間哺育
し、その間各試験区における下痢、軟便の発生率と体重
増加率を測定した。結果は表3に示すとおりである。Calf rearing test: Each of the above-prepared feeds having different galacto-oligosaccharide contents was fed to calves according to the following method to measure the incidence of diarrhea and loose stools and the weight gain rate. As a comparison, 0.01% by weight of galactooligosaccharide was prepared by reacting fructooligosaccharide or an enzyme in place of the galactooligosaccharide of Synthesis Example (Japanese Patent Publication No. 2-57902, prepared by the method of Example 1, the same applies hereinafter). And 0.1% by weight of the feed and a feed containing no oligosaccharide as a control were similarly fed. Test method: Each of the test groups consisting of 20 Holstein bulls one week old was fed with each of the above feeds and fed for 3 weeks, during which the incidence of diarrhea and loose stool and the rate of weight gain were measured. .. The results are shown in Table 3.
【0021】[0021]
【表3】 [Table 3]
【0022】表3にみられるとおり、本発明のガラクト
オリゴ糖を含有させた試験区では、対照ならびにフラク
トオリゴ糖や酵素法により調製したガラクトオリゴ糖を
同量含有させた各試験区に比べて下痢、軟便の発生率が
低く、体重増加率も良好であることが認められた。とり
わけ、0.01〜0.1重量%の範囲が有効であること
が判った。As can be seen from Table 3, in the test section containing the galacto-oligosaccharide of the present invention, diarrhea and loose stool were compared with the control section and each test section containing the same amount of fructooligosaccharide or the galacto-oligosaccharide prepared by the enzymatic method. It was found that the incidence of illness was low and the rate of weight gain was good. Especially, it has been found that the range of 0.01 to 0.1% by weight is effective.
【0023】実施例2 飼料へのガラクトオリゴ糖配合
飼料の調製と子豚飼育試験 合成例により調製して得たガラクトオリゴ糖粉末を、市
販人工乳A(商品名:ママ7ロイヤル、協同飼料社製)
及び人工乳B(商品名:ママ8サミツト、協同飼料社
製)にそれぞれ添加した配合飼料を用いて、下記に従っ
て子豚の飼育試験を行った。Example 2 Preparation of Feed Containing Galactooligosaccharide in Feed and Feeding Test for Piglets The galactooligosaccharide powder obtained by the synthesis example was used as a commercially available artificial milk A (trade name: Mama 7 Royal, manufactured by Kyodo Feed Co., Ltd.).
And a mixed feed added to each of the artificial milk B (trade name: Mama 8 Summit, manufactured by Kyodo Feed Co., Ltd.) were used to carry out a piglet rearing test in accordance with the following.
【0024】子豚の飼育試験:上述のようにしてガラク
トオリゴ糖を配合した飼料を次の方法にしたがって子豚
に給与し、体重の増加量、飼料の摂取量及び下痢、軟便
の発生状況を調べた。なお比較例として、人工乳A及び
人工乳Bに、合成例のガラクトオリゴ糖に代えて、フラ
クトオリゴ糖や酵素法により調製したガラクトオリゴ糖
をそれぞれ添加した飼料を給与して同様の測定を行い、
対照として、オリゴ糖無添加の人工乳A及び人工乳Bを
給与して同様の測定を行った。飼育は、21日令離乳子
豚(LW・D種)を一群16頭の群に分けて行い、0〜
15日間をA期として人工乳Aを給与し、16〜50日
間をB期として人工乳Bを給与した。結果は表4、表5
に示すとおりである。Breeding test of piglets: The feed containing the galacto-oligosaccharide as described above was fed to the piglets according to the following method to examine the weight gain, feed intake and diarrhea, loose stool generation. It was As a comparative example, artificial milk A and artificial milk B were fed with a feed containing fructo-oligosaccharides and galacto-oligosaccharides prepared by an enzymatic method, respectively, in place of the galacto-oligosaccharides of the synthesis example, and the same measurement was performed.
As a control, artificial milk A and artificial milk B without addition of oligosaccharide were fed and the same measurement was performed. The rearing is performed by dividing 21-day-old weaned piglets (LW / D breed) into groups of 16 pigs.
The artificial milk A was fed for 15 days as the A period, and the artificial milk B was fed for 16 to 50 days as the B period. The results are shown in Tables 4 and 5.
As shown in.
【0025】[0025]
【表4】 [Table 4]
【0026】[0026]
【表5】 [Table 5]
【0027】表4及び表5にみられるとおり、ガラクト
オリゴ糖を0.01〜0.1重量%配合した飼料を給与
した子豚の生育が対照およびフラクトオリゴ糖や酵素法
により調製したガラクトオリゴ糖を同量含有させた各試
験区に比べて、優れていることがわかる。As shown in Tables 4 and 5, the growth of piglets fed the feed containing 0.01 to 0.1% by weight of galactooligosaccharide was the same as that of the control and fructooligosaccharide or the galactooligosaccharide prepared by the enzymatic method. It can be seen that it is superior to each test section in which the amount was included.
【0028】実験例3 母豚基礎飼料に、合成例により調製して得たガラクトオ
リゴ糖粉末を各々添加した母豚飼料を調製した。比較例
として、合成例のガラクトオリゴ糖に代えて、フラクト
オリゴ糖や酵素法により調製したガラクトオリゴ糖をそ
れぞれ添加した飼料を、対照として、オリゴ糖無添加の
飼料を調製した。母豚基礎飼料の組成を表6に示した。Experimental Example 3 A sow feed was prepared by adding the galactooligosaccharide powder obtained by the synthesis example to the sow base feed. As a comparative example, a feed containing fructooligosaccharide or a galactooligosaccharide prepared by an enzymatic method in place of the galactooligosaccharide in the synthesis example was prepared, and a feed containing no oligosaccharide was prepared as a control. Table 6 shows the composition of the basic feed for sows.
【0029】[0029]
【表6】 [Table 6]
【0030】実験例4 実施例3で調製したガラクトオリゴ糖粉末を含む母豚飼
料を用い、以下の実験を行った。2ないし6経産の雌豚
のランドレス(Landrace)と雄のラージ・ホワイト(La
rge White)をかけ合わせた母豚を4頭毎の群に分け、
出産の1週間前から出産後25日間にわたり表7に示し
たように基礎飼料、ガラクトオリゴ頭含有飼料を与え
た。出産後25日にわたり、各実験区毎に子豚の体重及
び下痢の発生状況を観察した。Experimental Example 4 Using the mother pig feed containing the galactooligosaccharide powder prepared in Example 3, the following experiment was conducted. Two to six sows sow Landrace and male large white (La
Divide the sows that have been mixed with rge White) into groups of 4 pigs each,
As shown in Table 7, the basal diet and the galacto-oligo head-containing diet were fed from 1 week before delivery to 25 days after delivery. The body weight of piglets and the occurrence of diarrhea were observed for each experimental group over 25 days after delivery.
【0031】[0031]
【表7】 [Table 7]
【0032】本実験から、本発明の母豚用飼料組成物を
妊娠期間から哺乳期間を通して母豚に投与することによ
り哺乳期中の子豚の下痢発生率が低く、かつ子豚の体重
増加効果を有していることが明かになった。From this experiment, by administering the feed composition for sows of the present invention to sows throughout the pregnancy period and the feeding period, the diarrhea incidence rate of the piglets during the feeding period was low, and the weight gain effect of the piglets was obtained. It became clear what I was doing.
Claims (7)
量%配合してなる家畜用飼料。1. A feed for livestock, which comprises 0.01 to 0.1% by weight of galactooligosaccharide.
スである請求項1項記載の家畜用飼料。2. The feed for livestock according to claim 1, wherein the galactooligosaccharide is galactosyl lactose.
ースである請求項1項記載の家畜用飼料。3. The feed for livestock according to claim 1, wherein the galactooligosaccharide is digalactosyl lactose.
ステリグマトマイセス属またはシロバシディウム属に属
する微生物を乳糖または乳糖含有物に作用せしめて製造
されたガラクトオリゴ糖である請求項1乃至3項記載の
家畜用飼料。4. The livestock according to claim 1, wherein the galacto-oligosaccharide is a galacto-oligosaccharide produced by allowing a microorganism belonging to the genus Rhodotorula, the genus Sterigmatomyces or the genus Silobasidium to act on lactose or a substance containing lactose. Forage.
を加え無機酸の存在下、無水状態で加熱することにより
製造されたガラクトオリゴ糖である請求項1乃至3項記
載の家畜用飼料。5. The feed for livestock according to claim 1, wherein the galactooligosaccharide is a galactooligosaccharide produced by adding galactose to lactose and heating the mixture in the anhydrous state in the presence of an inorganic acid.
内1種類以上を含む請求項5記載の家畜用飼料。6. The livestock feed according to claim 5, wherein the lactose contains at least one of α-lactose, balanced lactose and β-lactose.
至6項記載の家畜用飼料。7. The livestock feed according to any one of claims 1 to 6, which is for mother pigs.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4026840A JPH05219897A (en) | 1992-02-13 | 1992-02-13 | Galactooligosaccharide-containing feed |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4026840A JPH05219897A (en) | 1992-02-13 | 1992-02-13 | Galactooligosaccharide-containing feed |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH05219897A true JPH05219897A (en) | 1993-08-31 |
Family
ID=12204470
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4026840A Pending JPH05219897A (en) | 1992-02-13 | 1992-02-13 | Galactooligosaccharide-containing feed |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH05219897A (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2291777A (en) * | 1994-07-13 | 1996-02-07 | Meiji Seika Kaisha | Method for elevating the rate of raising newborns by using feed for breeding sows |
EP0724840A1 (en) * | 1995-02-03 | 1996-08-07 | Meiji Seika Kaisha Ltd. | Method for improving breeding activity of boars by using feed for the breeding boars |
WO1997002829A2 (en) * | 1995-07-11 | 1997-01-30 | Abbott Laboratories | Use of indigestible oligosaccharides to prevent and treat diarrhea |
JP2004129609A (en) * | 2002-10-11 | 2004-04-30 | Kao Corp | Pet food |
WO2007138905A1 (en) | 2006-05-30 | 2007-12-06 | Ensuiko Sugar Refining Co., Ltd. | Intestinal eosinophil-suppressing composition |
WO2008081608A1 (en) | 2006-12-28 | 2008-07-10 | Yukiguni Maitake Co., Ltd. | Method of feeding mother pig |
JP2013051904A (en) * | 2011-09-02 | 2013-03-21 | Snow Brand Milk Products Co Ltd | Animal feed composition containing epilactose, animal mixed feed containing the same, and application of the same |
CN103099068A (en) * | 2013-03-07 | 2013-05-15 | 江西正邦科技股份有限公司 | Pregnant sow feed capable of reducing sow sand crack |
CN104642735A (en) * | 2013-11-15 | 2015-05-27 | 中国科学院过程工程研究所 | Complex carbohydrate preparation for forage, forage containing complex carbohydrate preparation, and use of forage |
-
1992
- 1992-02-13 JP JP4026840A patent/JPH05219897A/en active Pending
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5714181A (en) * | 1994-07-13 | 1998-02-03 | Meiji Seika Kaisha, Ltd. | Method for reducing the incidence of premature piglets by using feed containing oligosaccharides |
EP0710447A3 (en) * | 1994-07-13 | 1996-07-31 | Meiji Seika Kaisha | Method for elevating the rate of raising newborns by using feed for breeding sows |
GB2291777B (en) * | 1994-07-13 | 1998-08-19 | Meiji Seika Kaisha | Method for elevating the rate of raising newborns by using feed for breeding sows |
GB2291777A (en) * | 1994-07-13 | 1996-02-07 | Meiji Seika Kaisha | Method for elevating the rate of raising newborns by using feed for breeding sows |
FR2730133A1 (en) * | 1995-02-03 | 1996-08-09 | Meiji Seika Kaisha | FEEDING METHOD FOR IMPROVING THE REPRODUCTION ACTIVITY OF VERRATS |
EP0724840A1 (en) * | 1995-02-03 | 1996-08-07 | Meiji Seika Kaisha Ltd. | Method for improving breeding activity of boars by using feed for the breeding boars |
WO1997002829A3 (en) * | 1995-07-11 | 1997-03-27 | Abbott Lab | Use of indigestible oligosaccharides to prevent and treat diarrhea |
WO1997002829A2 (en) * | 1995-07-11 | 1997-01-30 | Abbott Laboratories | Use of indigestible oligosaccharides to prevent and treat diarrhea |
JP2004129609A (en) * | 2002-10-11 | 2004-04-30 | Kao Corp | Pet food |
WO2007138905A1 (en) | 2006-05-30 | 2007-12-06 | Ensuiko Sugar Refining Co., Ltd. | Intestinal eosinophil-suppressing composition |
WO2008081608A1 (en) | 2006-12-28 | 2008-07-10 | Yukiguni Maitake Co., Ltd. | Method of feeding mother pig |
JP2013051904A (en) * | 2011-09-02 | 2013-03-21 | Snow Brand Milk Products Co Ltd | Animal feed composition containing epilactose, animal mixed feed containing the same, and application of the same |
CN103099068A (en) * | 2013-03-07 | 2013-05-15 | 江西正邦科技股份有限公司 | Pregnant sow feed capable of reducing sow sand crack |
CN104642735A (en) * | 2013-11-15 | 2015-05-27 | 中国科学院过程工程研究所 | Complex carbohydrate preparation for forage, forage containing complex carbohydrate preparation, and use of forage |
CN104642735B (en) * | 2013-11-15 | 2018-05-22 | 中国科学院过程工程研究所 | A kind of feed is with glycoconjugate preparation and containing its feed and application |
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