JPH05203614A - Electrode-enclosure type detection element, and intermittent automatic analysis device and method - Google Patents
Electrode-enclosure type detection element, and intermittent automatic analysis device and methodInfo
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- JPH05203614A JPH05203614A JP4034475A JP3447592A JPH05203614A JP H05203614 A JPH05203614 A JP H05203614A JP 4034475 A JP4034475 A JP 4034475A JP 3447592 A JP3447592 A JP 3447592A JP H05203614 A JPH05203614 A JP H05203614A
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Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、酵素などの生物学的触
媒を担持する多孔質体と電極を利用して、血液や尿など
に含まれる検出対象物を検出することのできる、使い捨
て可能な検出要素、並びにその検出要素を用いる間欠型
自動分析装置及び方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention utilizes a porous material carrying a biological catalyst such as an enzyme and an electrode to detect a target substance contained in blood, urine or the like and is disposable. Detection element, and an intermittent automatic analyzer and method using the detection element.
【0002】[0002]
【従来の技術】血液や尿などの生体液体試料を連続的に
分析する装置としては、例えば、固定化酵素を担持した
ガラスビーズやキトサンビーズをチューブ状カラムに充
填した反応部を有するフローインジェクション装置が従
来から知られている。しかしながら、この装置では、通
常1〜2mm程度の狭い内径を有するカラムにビーズを充
填するので、酵素を固定化する操作が困難で、しかも煩
雑であった。また、カラム内に入った気泡を除去するの
が難しいので、気泡混入を防ぐためにカラム内を常に湿
潤状態に維持する必要があり、コンディショニングが困
難であった。更に、カラム内を常に湿潤状態に維持する
と、水中夾雑物の影響で酵素が失活し易くなって長期保
存が困難であると共に、検査試料中のタンパク質やバク
テリアなどの夾雑物により目詰まりを起こし易いという
欠点もあった。なお、フローインジェクション装置で
は、試料を分析系に注入するインジェクターや、試料を
反応部へ運ぶキャリアー液体も必要である。2. Description of the Related Art As an apparatus for continuously analyzing a biological liquid sample such as blood or urine, for example, a flow injection apparatus having a reaction section in which glass beads or chitosan beads carrying an immobilized enzyme are packed in a tubular column. Is conventionally known. However, in this apparatus, beads are packed in a column having a narrow inner diameter of about 1 to 2 mm, so that the procedure for immobilizing the enzyme is difficult and complicated. In addition, since it is difficult to remove the air bubbles that have entered the column, it is necessary to always keep the inside of the column in a wet state in order to prevent air bubbles from being mixed, and thus conditioning is difficult. Furthermore, if the column is always kept in a wet state, the enzyme will be easily deactivated due to the influence of contaminants in the water and long-term storage will be difficult, and the contaminants such as proteins and bacteria in the test sample will cause clogging. It also had the drawback of being easy. The flow injection device also requires an injector for injecting the sample into the analysis system and a carrier liquid for transporting the sample to the reaction section.
【0003】固定化酵素を利用する同様の装置として
は、電極の感応膜上に固定化酵素膜を装着し、その上か
らセルロース膜を被覆し、これらをO−リングで固定し
た酵素センサーが知られている。しかしながら、この種
の酵素センサーは、固定化酵素膜が電極表面に直接接触
しているので、酸化による劣化を受け易く、不安定で寿
命が短いだけでなく、固定化酵素膜を電極表面へ装着及
び脱着するのが煩雑であった。更に、電極表面に検査対
象物のみを到達させ、阻害物質を到達させないために緻
密な膜が必要であるが、そのような膜の調製は技術的に
難しく、量産が困難であった。As a similar device utilizing an immobilized enzyme, an enzyme sensor is known in which an immobilized enzyme membrane is mounted on a sensitive membrane of an electrode, a cellulose membrane is coated thereon, and these are immobilized by an O-ring. Has been. However, in this type of enzyme sensor, since the immobilized enzyme membrane is in direct contact with the electrode surface, it is susceptible to deterioration due to oxidation, is not stable and has a short life, and also mounts the immobilized enzyme membrane on the electrode surface. Also, it was troublesome to remove it. Furthermore, a dense film is required to allow only the inspection object to reach the electrode surface and not the inhibitor, but such a film is technically difficult to manufacture and mass production is difficult.
【0004】[0004]
【発明が解決しようとする課題】従って、本発明の目的
は、酵素の固定化操作が簡単で、酵素活性を長期間容易
に維持することができ、阻害物質による妨害を有効に阻
止することができ、更に、正確に、高精度に、しかも短
時間に測定を行なうことができる手段を提供することに
ある。また、本発明の別の目的は、試料や標準液を検出
系内に注入する手段や試料や標準液を検出系内に移送す
るキャリアー液を用いる必要のない自動分析手段を提供
することにある。SUMMARY OF THE INVENTION Therefore, the object of the present invention is that the enzyme immobilization operation is simple, the enzyme activity can be easily maintained for a long period of time, and the interference by the inhibitor can be effectively prevented. It is another object of the present invention to provide a means capable of performing measurement accurately, highly accurately, and in a short time. Another object of the present invention is to provide means for injecting a sample or standard solution into the detection system, or automatic analysis means that does not require the use of a carrier liquid for transferring the sample or standard solution into the detection system. ..
【0005】[0005]
【課題を解決するための手段】前記の目的は、本発明に
よる(1)試料入口及び試料出口を有するハウジング、
(2)試料中に含まれる検査対象物と反応して電極によ
って検出可能な変化を生じることのできる生物学的触媒
を多孔質支持体の表面全体に担持し、前記ハウジング内
に充填された多孔質反応体、(3)前記ハウジング内に
封入されると共に前記多孔質反応体と接触し、試料中に
含まれる検査対象物と生物学的触媒との反応による変化
を検出することのできる電極、及び(4)前記電極から
の信号を外部読取装置へ送ることのできる端子を備える
ことを特徴とする、検出要素によって達成することがで
きる。また、本発明は、(1)請求項1記載の検出要
素、(2)前記検出要素の試料入口から所定量の試料を
検出要素の反応室内に入れることのできる手段、(3)
前記反応室内に所定量の試料を停滞させて保持すること
のできる手段、(4)試料中に含まれる検査対象物と生
物学的触媒との反応によって発生した変化を検出した電
極からの信号を処理する装置、(5)検査済の試料を前
記反応室から試料出口を経て除去することのできる手
段、及び(6)試料の移動及び停滞の制御装置を含むこ
とを特徴とする、間欠型自動分析装置に関する。更に、
本発明は、前記の検出要素の試料入口から所定量の試料
を検出要素の反応室内に入れ、その試料を前記反応室内
に停滞させて保持し、試料中に含まれる検査対象物と生
物学的触媒との反応による変化を発生させ、その変化を
電極によって検出し、前記電極からの信号を読取装置へ
送り、そして検査済の試料を前記反応室から試料出口を
経て除去することを特徴とする、間欠型自動分析方法に
も関する。According to the present invention, there are provided (1) a housing having a sample inlet and a sample outlet,
(2) Porosity filled in the housing by supporting a biological catalyst capable of reacting with a test object contained in the sample and causing a change detectable by the electrode, on the entire surface of the porous support. (3) an electrode that is enclosed in the housing and is in contact with the porous reactant, and can detect a change due to a reaction between a test object contained in a sample and a biological catalyst, And (4) a sensing element, characterized in that it comprises a terminal capable of sending a signal from said electrode to an external reader. The present invention also provides (1) the detection element according to claim 1, (2) means for allowing a predetermined amount of sample to be introduced into the reaction chamber of the detection element from the sample inlet of the detection element, (3)
Means capable of holding a predetermined amount of sample in a stagnant state in the reaction chamber, (4) a signal from an electrode which detects a change caused by a reaction between a test object contained in the sample and a biological catalyst, A device for processing, (5) means capable of removing an inspected sample from the reaction chamber via a sample outlet, and (6) a device for controlling the movement and stagnation of the sample, the intermittent automatic Regarding analytical equipment. Furthermore,
According to the present invention, a predetermined amount of sample is put into the reaction chamber of the detection element from the sample inlet of the detection element, and the sample is held in the reaction chamber in a stagnant state, and the test object and biological substance contained in the sample It is characterized in that a change due to reaction with a catalyst is generated, the change is detected by an electrode, a signal from the electrode is sent to a reader, and an inspected sample is removed from the reaction chamber via a sample outlet. , Also relates to an intermittent automatic analysis method.
【0006】本発明による検出要素の代表的態様を図1
に示す。検出要素10は、前後に試料入口1及び試料出
口2を有するハウジング3からなる。ハウジング3は、
好ましくは非通気性で耐水性の材料からなる。ハウジン
グ3の内部には多孔質反応体4を充填した反応室5、及
び前記ハウジング3の内側壁面に設けた電極6がある。
多孔質反応体4は、多孔質支持体とその多孔質支持体の
表面全体に担持された生物学的触媒とからなる。電極6
はハウジングの外側表面に備えた端子7と電気的に連絡
している。試料入口1及び試料出口2と反応室5との間
に、必要によりメッシュ板8を設けることができる。A representative embodiment of the sensing element according to the invention is shown in FIG.
Shown in. The detection element 10 comprises a housing 3 having a sample inlet 1 and a sample outlet 2 at the front and back. The housing 3 is
It is preferably made of a non-breathable and water resistant material. Inside the housing 3 is a reaction chamber 5 filled with a porous reactant 4, and an electrode 6 provided on the inner wall surface of the housing 3.
The porous reactant 4 comprises a porous support and a biological catalyst supported on the entire surface of the porous support. Electrode 6
Are in electrical communication with terminals 7 provided on the outer surface of the housing. If necessary, a mesh plate 8 can be provided between the sample inlet 1 and the sample outlet 2 and the reaction chamber 5.
【0007】多孔質反応体4を形成する多孔質支持体の
材質は特に制限されるものではないが、繊維集合体(例
えば、不織布、フェルト、織物、編み物)若しくは発泡
体又はこれらの複合体が好ましく、不織布は3次元的な
立体構造を有するので特に好ましい。電極6と多孔質反
応体4とは互いに少なくとも1部分が接触していればよ
い。電極6は、金属箔のエッチング又はメッキ処理によ
ってハウジング内側壁面に設けても、又は金属板若しく
は金属棒などを多孔質反応体4で包み込んでもよい。The material of the porous support that forms the porous reaction body 4 is not particularly limited, but a fiber aggregate (for example, non-woven fabric, felt, woven fabric, knitted fabric) or foam or a composite thereof is used. It is particularly preferable that the non-woven fabric has a three-dimensional structure. At least a part of the electrode 6 and the porous reactant 4 may be in contact with each other. The electrode 6 may be provided on the inner wall surface of the housing by etching or plating a metal foil, or a metal plate, a metal rod, or the like may be wrapped with the porous reaction body 4.
【0008】本発明の検出要素は、任意の試料に含まれ
る各種の生物学的検査対象物の検出及び定量に用いるこ
とができるが、特には生物学的液体試料、例えば、血
液、血清、血漿などに含まれる脂質(例えば、コレステ
ロール、リン脂質、中性脂肪)、糖又は窒素化合物(例
えば、尿素、尿酸、クレアチニン)、尿などに含まれる
窒素化合物(例えば、尿酸、尿素態窒素)の検出及び定
量に用いる。本発明の検出要素で検査を実施する前に、
場合により、生物学的液体試料を生理食塩水などで希釈
したり、血液凝固防止剤などの薬剤で処理することもで
きる。The detection element of the present invention can be used for detecting and quantifying various biological test objects contained in an arbitrary sample, and in particular, a biological liquid sample such as blood, serum or plasma. Detection of lipids (eg cholesterol, phospholipids, neutral fats) contained in etc., sugars or nitrogen compounds (eg urea, uric acid, creatinine), nitrogen compounds contained in urine (eg uric acid, urea nitrogen) And used for quantification. Before carrying out the inspection with the detection element of the invention,
Optionally, the biological fluid sample can be diluted with saline or the like, or treated with a drug such as an anticoagulant.
【0009】本発明の検出要素に含まれる多孔質反応体
を構成する多孔質支持体の表面全体には、生物学的触媒
を担持させる。生物学的触媒としては、試料中に含まれ
る検査対象物と反応して電極によって検出可能な変化を
生じることのできるものであればよく、例えば、酵素、
オルガネラ、微生物、動植物細胞を用いることができ
る。本発明では、検査対象物の種類に応じて、生物学的
触媒を選択する。検査対象物と1種又はそれ以上の生物
学的触媒(特には酵素)との反応により、最終的に過酸
化水素又はアンモニアを発生する組合せを選択するのが
好ましい。それらの組合せとしては既に多くの例が知ら
れているが、代表例を挙げると、グルコース−グルコー
スオキシダーゼ(過酸化水素)、ピルビン酸−ピルビン
酸オキシダーゼ(過酸化水素)、尿酸−ウリカーゼ(過
酸化水素)、尿素−ウレアーゼ(アンモニア)、中性脂
肪−リパーゼとグリセロールオキシダーゼ(過酸化水
素)、コレステロール−コレステロールオキシダーゼ
(過酸化水素)、Lアミノ酸−Lアミノ酸オキシダーゼ
(過酸化水素)、エタノール−アルコールオキシダーゼ
(過酸化水素)などがある。また、検査対象物と生物学
的触媒との反応生成物の種類に応じて電極を選択する。
例えば、過酸化水素生成反応に対しては白金極や炭素極
を用いてアンペロメトリーにより反応を検出することが
できる。また、アンモニア生成反応に対してはアンモニ
ア電極やイオン選択性電界効果トランジスタ(FET)
電極などを好適に利用することができる。A biological catalyst is supported on the entire surface of the porous support constituting the porous reactant contained in the detection element of the present invention. The biological catalyst may be any as long as it can react with an object to be tested contained in a sample to cause a change detectable by an electrode, for example, an enzyme,
Organelles, microorganisms, animal and plant cells can be used. In the present invention, a biological catalyst is selected according to the type of test object. It is preferred to select a combination that will eventually produce hydrogen peroxide or ammonia by the reaction of the test object with one or more biological catalysts (especially enzymes). Although many examples are already known as combinations thereof, typical examples are glucose-glucose oxidase (hydrogen peroxide), pyruvate-pyruvate oxidase (hydrogen peroxide), and urate-uricase (peroxidation). Hydrogen), urea-urease (ammonia), neutral fat-lipase and glycerol oxidase (hydrogen peroxide), cholesterol-cholesterol oxidase (hydrogen peroxide), L amino acid-L amino acid oxidase (hydrogen peroxide), ethanol-alcohol oxidase (Hydrogen peroxide) etc. Further, the electrode is selected according to the type of the reaction product of the test object and the biological catalyst.
For example, with respect to the hydrogen peroxide production reaction, the reaction can be detected by amperometry using a platinum electrode or a carbon electrode. Also, for the ammonia production reaction, an ammonia electrode or an ion selective field effect transistor (FET) is used.
An electrode or the like can be preferably used.
【0010】生物学的触媒を多孔質支持体に固定させ、
担持させる方法としては、任意の固定化法を利用するこ
とができるが、例えば、多孔質支持体の表面に直接又は
間接に生物学的触媒を共有結合又はイオン結合などによ
り結合する担体結合法(例えば、特開昭60-224618 号公
報記載の方法)や、絹フィブロインなどの高分子ゲルの
格子内や樹脂膜内に生物学的触媒を閉じ込めて固定化す
る包括法(特開平2-245189号又は特開昭60-120988 号各
公報記載の方法)などを使用することができる。特に、
特開平2-245189号公報記載の方法により、生物学的触媒
を含む絹フィブロイン水溶液を繊維集合体(特に、親水
性繊維を主体とするもの)に含浸させて乾燥し、アルコ
ールで不溶化処理すると、生物学的触媒が絹フィブロイ
ンのゲル層に内包されて固定されると共に、絹フィブロ
インのゲル層は、酵素タンパク質などの大きな分子を通
さず、反応基質となる検査対象物などの小さな分子を通
過させることができるので、生物学的触媒を固定化する
手段として望ましい。Immobilizing the biological catalyst on a porous support,
As a method for supporting, any immobilization method can be used, but for example, a carrier binding method in which a biological catalyst is directly or indirectly bound to the surface of a porous support by a covalent bond or an ionic bond ( For example, the method described in JP-A-60-224618) or a comprehensive method for entrapping and immobilizing a biological catalyst in a lattice of a polymer gel such as silk fibroin or in a resin film (JP-A-2-245189). Alternatively, the methods described in JP-A-60-120988, etc.) can be used. In particular,
According to the method described in JP-A-2-245189, a silk fibroin aqueous solution containing a biological catalyst is impregnated into a fiber assembly (particularly one mainly composed of hydrophilic fibers), dried, and insolubilized with alcohol, The biological catalyst is encapsulated and fixed in the silk fibroin gel layer, and the silk fibroin gel layer does not pass large molecules such as enzyme proteins but small molecules such as the test object that is the reaction substrate. Therefore, it is desirable as a means for immobilizing a biological catalyst.
【0011】次に、本発明による検出要素10の使用方
法、並びに本発明の自動分析装置及び自動分析方法を図
2に示す態様に沿って説明する。まず、流路切換バルブ
11を洗浄液槽12と連絡させ、洗浄液槽12内の洗浄
液(例えば、生理食塩水)を検出要素10に送り、反応
室5内に充填する。次に、流路切換バルブ11を標準液
槽13と連絡させる。標準液槽13内に保存されている
標準液は、試料槽14内の試料に含まれる可能性のある
検査対象物の対照となる、既知濃度の標準試薬である。
ポンプ15を運転して、反応室5内の洗浄液を除去する
と共に、反応室5内に前記標準液を充填してポンプ15
を停止する。標準液内の既知濃度の標準検査対象物と生
物学的触媒との反応が進行し、検出可能な変化(例え
ば、反応生成物の増加又は反応消費物の減少)が起き
る。それらの変化を電極6で検知し、端子7を介してデ
ータ処理制御装置16へ送る。前記の標準検査対象物と
生物学的触媒との反応が終了するか、或いは所定の時間
反応させてから、流路切換バルブ11を洗浄液槽12と
連絡させ、ポンプ15により標準液を除去すると共に洗
浄液を反応室5に送り、充填させる。Next, a method of using the detection element 10 according to the present invention, and an automatic analyzer and an automatic analysis method of the present invention will be described with reference to the embodiment shown in FIG. First, the flow path switching valve 11 is connected to the cleaning liquid tank 12, and the cleaning liquid (for example, physiological saline) in the cleaning liquid tank 12 is sent to the detection element 10 to fill the reaction chamber 5. Next, the flow path switching valve 11 is connected to the standard liquid tank 13. The standard solution stored in the standard solution tank 13 is a standard reagent having a known concentration, which serves as a control for the test object that may be contained in the sample in the sample tank 14.
The pump 15 is operated to remove the cleaning liquid in the reaction chamber 5, and the reaction chamber 5 is filled with the standard liquid to pump the pump 15.
To stop. The reaction between the standard test analyte of known concentration in the standard solution and the biological catalyst proceeds, causing a detectable change (eg, increased reaction products or decreased reaction consumption). These changes are detected by the electrode 6 and sent to the data processing controller 16 via the terminal 7. After the reaction between the standard test object and the biological catalyst is completed or after reacting for a predetermined time, the flow path switching valve 11 is connected to the cleaning liquid tank 12, and the standard liquid is removed by the pump 15. The cleaning liquid is sent to the reaction chamber 5 and filled therein.
【0012】続いて、流路切換バルブ11を試料槽14
と連絡させ、ポンプ15により洗浄液を除去すると共に
液体試料を反応室5に送り、反応室5が液体試料で充填
された段階でポンプ15を停止する。液体試料内に検査
対象物が含まれていると、それらは多孔質支持体に固定
されている生物学的触媒と接触するので、両者間の反応
が進行して検出可能な変化が起きる。前記の反応が終了
するまで、或いは所定の時間反応させるまで、試料を反
応室5内に留め、その間の変化を前記と同様に電極6で
検知し、端子7を介してデータ処理制御装置16へ送
る。データ処理制御装置16で処理された検出値は、記
録器17で記録され、適当な形式で表示される。反応室
5から除去された各種の液は、廃液槽18に送られる。Subsequently, the flow path switching valve 11 is connected to the sample tank 14
The cleaning liquid is removed by the pump 15 and the liquid sample is sent to the reaction chamber 5, and the pump 15 is stopped when the reaction chamber 5 is filled with the liquid sample. When the liquid sample contains test substances, they come into contact with the biological catalyst immobilized on the porous support, and the reaction between the two proceeds to cause a detectable change. The sample is kept in the reaction chamber 5 until the above reaction is completed or until it is reacted for a predetermined time, and the change during that time is detected by the electrode 6 in the same manner as described above, and is sent to the data processing control device 16 via the terminal 7. send. The detected value processed by the data processing control device 16 is recorded by the recorder 17 and displayed in an appropriate format. The various liquids removed from the reaction chamber 5 are sent to the waste liquid tank 18.
【0013】1つの試料の検査が終了したら、再び流路
切換バルブ11を洗浄液槽12と連絡させて反応室5を
洗浄液で洗浄し、前記の操作を繰り返すことができる。
或いは検査済の検出要素10を取り出し、新しい検出要
素10と取り替えてもよい。流路切換バルブ11及びポ
ンプ15の作動は、データ処理制御装置16によって制
御することができる。試料槽14を次々に新しいものに
替えていけば、連続自動分析を行なうことができ、複数
の検出要素10と複数の標準液槽13を同時に設置する
ことにより、複数の検査対象物の自動分析を行なうこと
ができる。When the inspection of one sample is completed, the flow path switching valve 11 is again brought into contact with the cleaning liquid tank 12 to clean the reaction chamber 5 with the cleaning liquid, and the above operation can be repeated.
Alternatively, the inspected detection element 10 may be taken out and replaced with a new detection element 10. The operation of the flow path switching valve 11 and the pump 15 can be controlled by the data processing control device 16. If the sample tank 14 is replaced with a new one one after another, continuous automatic analysis can be performed. By simultaneously installing a plurality of detection elements 10 and a plurality of standard solution tanks 13, automatic analysis of a plurality of test objects can be performed. Can be done.
【0014】[0014]
【発明の効果】本発明による検出要素の反応室に充填さ
れている多孔質反応体は、酵素の固定化操作が簡単で、
酵素活性を長期間容易に維持することができ、阻害物質
による妨害を有効に阻止することができ、更に、正確
に、高精度に、しかも短時間に測定を行なうことができ
る。また、本発明による間欠型分析装置及び方法によれ
ば、従来のフロー型自動分析方式とは異なり、試料や標
準液を分析系内に注入する手段(例えばマイクロシリン
ジ)や試料や標準液を分析系内に移送するキャリアー液
が不要になる。また、反応室内で反応時間を自由に設定
することができるので、各種の自動検査に用いることが
でき、更に連続法にも適している。The porous reactant filled in the reaction chamber of the detection element according to the present invention has a simple procedure for immobilizing the enzyme,
The enzyme activity can be easily maintained for a long period of time, the interference by the inhibitory substance can be effectively prevented, and further, the measurement can be performed accurately, highly accurately, and in a short time. Further, according to the intermittent analyzer and method according to the present invention, unlike the conventional flow-type automatic analysis method, a means (for example, a microsyringe) for injecting a sample or standard solution into an analysis system, or a sample or standard solution is analyzed. The carrier liquid transferred into the system becomes unnecessary. Further, since the reaction time can be freely set in the reaction chamber, it can be used for various automatic inspections and is also suitable for the continuous method.
【図1】本発明の検出要素の1態様の断面図である。1 is a cross-sectional view of one embodiment of the sensing element of the present invention.
【図2】本発明の分析装置の1態様の説明図である。FIG. 2 is an explanatory diagram of one mode of the analyzer of the present invention.
1・・試料入口;2・・試料出口;3・・ハウジング;
4・・多孔質反応体;5・・反応室;6・・電極;7・
・端子;8・・メッシュ板;10・・検出要素;11・
・流路切換バルブ;12・・洗浄液槽;13・・標準液
槽;14・・試料槽;15 ポンプ;16・・データ処
理制御装置;17・・記録器;18・・廃液槽1 ... Sample inlet; 2 ... Sample outlet; 3 ... Housing;
4 ... Porous reactant; 5 ... Reaction chamber; 6 ... Electrode; 7 ...
・ Terminal; 8 ・ ・ Mesh plate; 10 ・ ・ Detection element; 11 ・
・ Flow switching valve; 12 ・ ・ Washing liquid tank; 13 ・ ・ Standard liquid tank; 14 ・ ・ Sample tank; 15 Pumps; 16 ・ ・ Data processing control device; 17 ・ ・ Recorder; 18 ・ ・ Waste liquid tank
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 FI 技術表示箇所 G01N 35/08 A 8310−2J 7235−2J G01N 27/46 336 B 7235−2J 336 Z ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification number Office reference number FI technical display location G01N 35/08 A 8310-2J 7235-2J G01N 27/46 336 B 7235-2J 336 Z
Claims (3)
ウジング、(2)試料中に含まれる検査対象物と反応し
て電極によって検出可能な変化を生じることのできる生
物学的触媒を多孔質支持体の表面全体に担持し、前記ハ
ウジング内に充填された多孔質反応体、(3)前記ハウ
ジング内に封入されると共に前記多孔質反応体と接触
し、試料中に含まれる検査対象物と生物学的触媒との反
応による変化を検出することのできる電極、及び(4)
前記電極からの信号を外部読取装置へ送ることのできる
端子を備えることを特徴とする、検出要素。1. (1) A housing having a sample inlet and a sample outlet, (2) a porous biological catalyst capable of reacting with a test object contained in the sample and causing a change detectable by an electrode. A porous reaction material which is carried on the entire surface of the support and is filled in the housing, and (3) an inspection target which is enclosed in the housing and is in contact with the porous reaction material, and which is contained in the sample. An electrode capable of detecting a change due to a reaction with a biological catalyst, and (4)
A detection element comprising a terminal capable of sending a signal from the electrode to an external reader.
前記検出要素の試料入口から所定量の試料を検出要素の
反応室内に入れることのできる手段、(3)前記反応室
内に所定量の試料を停滞させて保持することのできる手
段、(4)試料中に含まれる検査対象物と生物学的触媒
との反応によって発生した変化を検出した電極からの信
号を処理する装置、(5)検査済の試料を前記反応室か
ら試料出口を経て除去することのできる手段、及び
(6)試料の移動及び停滞の制御装置を含むことを特徴
とする、間欠型自動分析装置。2. The detecting element according to claim 1, (2)
Means capable of introducing a predetermined amount of sample into the reaction chamber of the detection element from the sample inlet of the detection element, (3) Means capable of retaining and retaining a predetermined amount of sample in the reaction chamber, (4) Sample A device for processing a signal from an electrode which detects a change generated by a reaction between a test object contained therein and a biological catalyst, (5) removing an inspected sample from the reaction chamber through a sample outlet. And (6) a sample movement and stagnation control device, an intermittent automatic analyzer.
所定量の試料を検出要素の反応室内に入れ、その試料を
前記反応室内に停滞させて保持し、試料中に含まれる検
査対象物と生物学的触媒との反応による変化を発生さ
せ、その変化を電極によって検出し、前記電極からの信
号をデータ処理制御装置へ送り、そして検査済の試料を
前記反応室から試料出口を経て除去することを特徴とす
る、間欠型自動分析方法。3. An object to be inspected contained in the sample, wherein a predetermined amount of sample is put into the reaction chamber of the detection element from the sample inlet of the detection element according to claim 1, and the sample is held in the reaction chamber while being held therein. Change due to the reaction of the biological catalyst with the biological catalyst, the change is detected by the electrode, the signal from the electrode is sent to the data processing controller, and the inspected sample is removed from the reaction chamber via the sample outlet. An intermittent automatic analysis method characterized by:
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4034475A JPH05203614A (en) | 1992-01-25 | 1992-01-25 | Electrode-enclosure type detection element, and intermittent automatic analysis device and method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4034475A JPH05203614A (en) | 1992-01-25 | 1992-01-25 | Electrode-enclosure type detection element, and intermittent automatic analysis device and method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH05203614A true JPH05203614A (en) | 1993-08-10 |
Family
ID=12415282
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4034475A Pending JPH05203614A (en) | 1992-01-25 | 1992-01-25 | Electrode-enclosure type detection element, and intermittent automatic analysis device and method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH05203614A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07270377A (en) * | 1994-03-31 | 1995-10-20 | Toto Ltd | Polarograph analyser equipped with disposable flow cell |
JP2010008113A (en) * | 2008-06-25 | 2010-01-14 | Hitachi High-Technologies Corp | Flow injection analyzer |
-
1992
- 1992-01-25 JP JP4034475A patent/JPH05203614A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07270377A (en) * | 1994-03-31 | 1995-10-20 | Toto Ltd | Polarograph analyser equipped with disposable flow cell |
JP2010008113A (en) * | 2008-06-25 | 2010-01-14 | Hitachi High-Technologies Corp | Flow injection analyzer |
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