JPH05184350A - System for automatic counting of bacterial cell suspending in air - Google Patents
System for automatic counting of bacterial cell suspending in airInfo
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- JPH05184350A JPH05184350A JP563292A JP563292A JPH05184350A JP H05184350 A JPH05184350 A JP H05184350A JP 563292 A JP563292 A JP 563292A JP 563292 A JP563292 A JP 563292A JP H05184350 A JPH05184350 A JP H05184350A
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- air
- bacteria
- condensed water
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- fluorescence
- Prior art date
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明はバイオクリーンルーム
内の空中浮遊菌の菌数測定に適用される空中浮遊細菌の
自動計測システムに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic measuring system for airborne bacteria, which is applied to measure the number of airborne bacteria in a bioclean room.
【０００２】[0002]
【従来の技術】空気中にはさまざまな微生物が数多く浮
遊しているが、その大部分は土壌、動植物の腐植***
物、衣服の細片等塵埃に付着して存在している。従っ
て、空中菌の数はそれら塵埃量に比例する。従来の空中
浮遊細菌の計測方法には、浮遊細菌の自然落下を利用し
た空中落下菌試験が用いられていた。この空中落下菌試
験は、普通寒天平板培地上に一定時間内に落下した菌を
補集し、培養後発生した集落数をもって表わすものであ
り、下記に説明する。2. Description of the Related Art A large number of various microorganisms are suspended in the air, but most of them are present on soil, humus excrement of animals and plants, and dust such as small pieces of clothes. Therefore, the number of airborne bacteria is proportional to the amount of dust. As a conventional method for measuring airborne bacteria, an airborne bacteria test using free fall of airborne bacteria has been used. This drop-in-air test is performed by collecting the bacteria that have fallen on a regular agar plate medium within a certain period of time and expressing the number of colonies generated after culturing, which will be described below.
【０００３】普通寒天培地を調製し、オートクレーブ殺
菌を例えば１２０℃で１５分間行なう。この寒天培地が
注入された殺菌ペトリ皿３枚を試験対象とする場所に設
置し、同時にふたを取って任意時間、例えば５分間露出
静置する。An ordinary agar medium is prepared, and autoclave sterilization is carried out, for example, at 120 ° C. for 15 minutes. Three sterilized Petri dishes in which this agar medium has been injected are set at the test target locations, the lids are removed at the same time, and the plates are left to stand for an arbitrary time, for example, 5 minutes.
【０００４】露出静置後、上記普通寒天培地を恒温機中
にて２〜３日間培養し、発生したコロニー（集落）数を
肉眼で計数する。そして、各培地のコロニー数を平均し
てペトリ皿一枚当りの落下菌数とする。After the exposure and standing, the ordinary agar medium was cultured in a thermostat for 2 to 3 days, and the number of colonies (colts) generated was visually counted. Then, the number of colonies in each medium is averaged to obtain the number of dropped bacteria per Petri dish.
【０００５】落下菌数の評価は、Ａ（３０以下）、Ｂ
（３１〜７４）、Ｃ（７５〜１５０）、Ｄ（１５１〜２
９９）、Ｅ（３００以下）に分けられ、室内では３０以
下ならば良好、１５０以上では不適当とされる。Evaluation of the number of fallen bacteria is A (30 or less), B
(31 to 74), C (75 to 150), D (151 to 2)
99) and E (300 or less). Indoors, 30 or less is good, and 150 or more is inappropriate.
【０００６】[0006]
【発明が解決しようとする課題】しかし、上記従来の空
中浮遊細菌の計測方法では、細菌による空気汚染度を検
出するのに長時間必要とするため、製造又は加工等を行
なう試験対象場所においては適切な対応をとることが困
難となる。However, in the above-mentioned conventional method for measuring airborne bacteria, it takes a long time to detect the degree of air pollution by bacteria. It will be difficult to take appropriate measures.
【０００７】又、医薬品工場等では高いクリーン度が要
求されるが、通常このような高いクリーン度を要求され
る場所では空中落下菌が少なく、従来のペトリ皿を用い
る培養法では、ペトリ皿の開放時間を指数的に増加しな
ければ十分な測定精度が得られない。更に、ペトリ皿の
設置場所によっては空気流れ分布等が異なるため測定に
影響を与え、測定値の信頼性が低下する。[0007] In pharmaceutical factories and the like, high cleanliness is required, but normally, in places where such high cleanliness is required, airborne bacteria are small, and in the conventional culture method using a Petri dish, the Sufficient measurement accuracy cannot be obtained unless the opening time is increased exponentially. Further, the air flow distribution and the like differ depending on the installation location of the Petri dish, which affects the measurement and reduces the reliability of the measured value.
【０００８】この発明は上記実情に鑑みてなされたもの
で、測定の自動化を行ない、菌数測定に要する時間を短
縮し、更に測定精度を向上し得る空中浮遊細菌の自動計
測システムを提供することを目的とする。The present invention has been made in view of the above circumstances, and provides an automatic measurement system for airborne bacteria that can automate the measurement, shorten the time required for measuring the number of bacteria, and further improve the measurement accuracy. With the goal.
【０００９】[0009]
【課題を解決するための手段】この発明に係る空中浮遊
細菌の自動計測システムは、測定対象とする空気中の浮
遊細菌を液滴に付着させて補集液とする補集手段と、こ
の補集手段より生成された上記補集液に沸騰トリス緩衝
液を添加して検液を生成する検液生成手段と、この検液
生成手段により生成された検液を励起させる励起手段
と、この励起手段により発光した蛍光の蛍光量を計測す
る蛍光量計測手段と、この蛍光量計測手段により計測さ
れた蛍光量から測定対象である上記空気中の浮遊細菌を
算出する算出手段とを具備することを特徴とする。An automatic measuring system for airborne bacteria according to the present invention comprises a collecting means for adhering airborne bacteria in the air to be measured to a droplet to form a collecting liquid, and a collecting means for collecting the collecting liquid. A test solution generating means for generating a test solution by adding a boiling Tris buffer solution to the collecting solution generated by the collecting means, an excitation means for exciting the test solution generated by the test solution generating means, and this excitation A fluorescence amount measuring means for measuring the fluorescence amount of the fluorescence emitted by the means, and a calculating means for calculating the floating bacteria in the air which is a measurement target from the fluorescence amount measured by the fluorescence amount measuring means. Characterize.
【００１０】[0010]
【作用】クリーンルームの循環エアは、コンプレッサに
より圧縮された後、加湿器で噴霧される。これにより循
環エア中の浮遊細菌は、噴霧表面に付着する。噴霧は膨
脹タービンによって凝縮水となり、この凝縮水は遠心分
離器により清浄エアと分離される。この清浄エアはクリ
ーンルームに返送される。The circulating air in the clean room is compressed by the compressor and then sprayed by the humidifier. As a result, airborne bacteria in the circulating air adhere to the spray surface. The mist becomes condensed water by the expansion turbine, and this condensed water is separated from the clean air by the centrifugal separator. This clean air is returned to the clean room.
【００１１】浮遊細菌を含む上記凝縮水に沸騰トリス緩
衝液を添加して反応管においてＡＴＰ（アデノシン３リ
ン酸）を抽出する。ＡＴＰが抽出された凝縮水は、冷却
管により常温まで冷却され、測定キュベットに連続的に
送液される。A boiling Tris buffer is added to the condensed water containing floating bacteria to extract ATP (adenosine triphosphate) in a reaction tube. The condensed water from which ATP has been extracted is cooled to room temperature by a cooling pipe and continuously sent to the measurement cuvette.
【００１２】測定キュベットには、ＫｒＦレーザから励
起光が照射され、これによりＡＴＰが励起し、ＡＴＰが
脱リン化され、ＡＭＰ（アデノシン１リン酸）とピロリ
ン酸を形成する。この際、フォトンが放出され、ピーク
波長が５６２ｎｍの蛍光を発する。この蛍光は、光電子
倍増管に送られ、蛍光量を測定され、これより菌数が算
出される。The measurement cuvette is irradiated with excitation light from a KrF laser, which excites ATP and dephosphorizes ATP, forming AMP (adenosine monophosphate) and pyrophosphate. At this time, photons are emitted and fluorescence with a peak wavelength of 562 nm is emitted. This fluorescence is sent to a photomultiplier tube, the amount of fluorescence is measured, and the number of bacteria is calculated from this.
【００１３】[0013]
【実施例】以下、図面を参照してこの発明の一実施例を
説明する。この発明に係る空中浮遊細菌の自動計測シス
テムの構成を図１に示す。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 shows the configuration of an automatic measurement system for airborne bacteria according to the present invention.
【００１４】コンプレッサ１はモータ２を駆動すること
により作動し、医薬品工場等のクリーンルーム３におい
て循環された空気を循環エア４として取込み、これを圧
縮後、加湿器５に送出する。加湿器５は、上記循環エア
４に対して噴霧を行ない、循環エア４中の浮遊細菌を噴
霧表面に付着させ、タービン６に送る。The compressor 1 operates by driving a motor 2, takes in the air circulated in a clean room 3 such as a pharmaceutical factory as circulation air 4, compresses it, and sends it to a humidifier 5. The humidifier 5 sprays the circulating air 4 to attach the floating bacteria in the circulating air 4 to the surface of the spray, and sends it to the turbine 6.
【００１５】タービン６はモータ７を駆動することによ
り作動し、表面に浮遊細菌を付着した上記噴霧を断熱膨
脹させて凝縮水８とする。遠心分離器９は、遠心分離を
用いて凝縮水８と清浄エア１０とを分離し、清浄エア１
０のみを再びクリーンルーム３に送る。凝縮水８には沸
騰トリス緩衝液１１が添加され、反応管１２に送液され
る。反応管１２は、この沸騰トリス緩衝液１１を添加さ
れた凝縮水８からＡＴＰ（アデノシン３リン酸）を加熱
抽出し、検液とする。The turbine 6 operates by driving a motor 7 to adiabatically expand the above-mentioned spray having floating bacteria adhered to the surface thereof to form condensed water 8. The centrifuge 9 separates the condensed water 8 and the clean air 10 by using centrifugal separation,
Only 0 is sent to the clean room 3 again. The boiling Tris buffer 11 is added to the condensed water 8 and sent to the reaction tube 12. The reaction tube 12 heat-extracts ATP (adenosine triphosphate) from the condensed water 8 to which the boiling Tris buffer solution 11 has been added to obtain a test solution.
【００１６】冷却管１３は、上記ＡＴＰが抽出された検
液を常温まで冷却し、測定キュベット１４に連続的に送
液する。測定キュベット１４にはＫｒＦレーザ１５から
レンズ１６を介して励起光が照射され、上記検液にＡＭ
Ｔ（アデノシン１リン酸）とピロリン酸を生成させる。
この際、検液からフォトンが放出されピーク波長５６２
ｎｍの蛍光１７を発する。The cooling pipe 13 cools the test liquid from which ATP has been extracted to room temperature and continuously feeds it to the measurement cuvette 14. The measurement cuvette 14 is irradiated with the excitation light from the KrF laser 15 through the lens 16, and the test solution is AM
It produces T (adenosine monophosphate) and pyrophosphate.
At this time, photons are emitted from the test solution and the peak wavelength 562 is reached.
Fluorescent light 17 of nm is emitted.
【００１７】上記測定キュベット１４の近傍にはレンズ
１８が付設され、蛍光１７を集光する。更に、レンズ１
８による集光部近傍には、スリット１９及びレンズ２０
が設けられ、蛍光１７をフィルタ２１に送出する。フィ
ルタ２１は、特定の波長のみを通過させ、光電子倍増管
２２に出力する。光度計・記録計２３は、この光電子倍
増管２２の出力から蛍光量を計測積算し、この蛍光量か
ら菌数を算出する。A lens 18 is attached in the vicinity of the measuring cuvette 14 to collect the fluorescent light 17. Furthermore, lens 1
A slit 19 and a lens 20 are provided in the vicinity of the light condensing part by 8.
Is provided and sends the fluorescent light 17 to the filter 21. The filter 21 passes only a specific wavelength and outputs it to the photomultiplier tube 22. The photometer / recorder 23 measures and integrates the fluorescence amount from the output of the photomultiplier tube 22, and calculates the number of bacteria from this fluorescence amount.
【００１８】次に上記実施例の動作を説明する。クリー
ンルーム３中の循環エア４は、コンプレッサ１によって
圧縮された後、加湿器５にて加湿される。これにより、
注入された噴霧表面に循環エア４中の浮遊細菌が付着さ
れる。更に膨脹タービン６における断熱膨脹によって凝
縮水８が生成され、これを遠心分離器９で清浄エア１０
と分離される。清浄エア１０はクリーンルーム３に返送
される。Next, the operation of the above embodiment will be described. The circulating air 4 in the clean room 3 is compressed by the compressor 1 and then humidified by the humidifier 5. This allows
Suspended bacteria in the circulating air 4 adhere to the injected spray surface. Further, adiabatic expansion in the expansion turbine 6 produces condensed water 8, which is then separated by a centrifugal separator 9 into clean air 10
And separated. The clean air 10 is returned to the clean room 3.
【００１９】上記分離された凝縮水８は、沸騰トリス緩
衝液１１が添加されて反応管１２に送液される。沸騰ト
リス緩衝液１１が添加された凝縮水８は、反応管１２に
おいてＡＴＰを加熱抽出され、検液とされる。検液は、
冷却管１３に送液されて常温まで冷却され、更に測定キ
ュベット１４に連続的に送液される。The condensed water 8 thus separated is added with the boiling Tris buffer 11 and sent to the reaction tube 12. The condensed water 8 to which the boiling Tris buffer solution 11 has been added has ATP heated and extracted in the reaction tube 12 to be used as a test solution. The test solution is
The liquid is sent to the cooling pipe 13, cooled to room temperature, and further continuously sent to the measurement cuvette 14.
【００２０】測定キュベット１４に送られた検液には、
ＫｒＦレーザ１５からの励起光が照射され、これにより
ＡＭＴとピロリン酸を生成する。この際、上記検液より
フォトンが放出され、ピーク波長５６２ｎｍの蛍光１７
が発光される。この蛍光１７は、レンズ１７によって集
光されてスリット１９に照射され、更に、レンズ２０を
介してフィルタ２１に送られる。このフィルタ２１によ
り、上記蛍光１７は特定の波長のみが通過し、光電子倍
増管２２に入力される。光電子倍増管２２及び光度計・
記録計２３は上記フィルタ２１を通過した蛍光から蛍光
量を計測積算してこの蛍光量から菌数を算出する。The test liquid sent to the measurement cuvette 14 includes
Excitation light from the KrF laser 15 is irradiated to generate AMT and pyrophosphate. At this time, photons are emitted from the test solution and fluorescence 17 having a peak wavelength of 562 nm is emitted.
Is emitted. The fluorescent light 17 is condensed by the lens 17, irradiated on the slit 19, and further sent to the filter 21 via the lens 20. Only a specific wavelength of the fluorescent light 17 passes through the filter 21 and is input to the photomultiplier tube 22. Photomultiplier tube 22 and photometer
The recorder 23 measures and integrates the fluorescence amount from the fluorescence that has passed through the filter 21 and calculates the number of bacteria from this fluorescence amount.
【００２１】次に上記実施例の動作を具体的数値を用い
て説明する。クリーンルーム３中の循環エア４に液体培
養した枯草菌を１０3 〜１０6 個／ft3 air（クラス、
1,000 〜1,000,000 ）になるように噴霧注入する。この
循環エア４は、コンプレッサ１にて１．８Kg/cm 2 に圧
縮され、加湿器５で絶対温度２．７３wt％まで加湿され
た後、膨脹タービン６で常圧まで減圧される。これによ
って凝縮水８が発生する。Next, the operation of the above embodiment will be described using specific numerical values. 10 3 of liquid-cultured Bacillus subtilis in the circulating air 4 in the clean room 3 ~ 10 6 Pieces / ft 3 air (class,
Spray injection so that the amount becomes 1,000 to 1,000,000). This circulating air 4 is 1.8 kg / cm 2 in the compressor 1. And is humidified by the humidifier 5 to an absolute temperature of 2.73 wt%, and then decompressed by the expansion turbine 6 to a normal pressure. As a result, condensed water 8 is generated.
【００２２】その後、上記循環エア４は、遠心分離器９
にて凝縮水８と清浄エア１０に分離される。上記数値条
件の下で凝縮水８は、約０．７３cc／ft3 air補集され
た。ここで、上記処理前後における浮遊微粒子の濃度計
測結果の一例を図２に示す。通常、細菌は０．５μｍ以
上の大きさを有することから、同図より９８％以上の細
菌が凝縮水８中に補集されていることになる。Thereafter, the circulating air 4 is supplied to the centrifugal separator 9
Is separated into condensed water 8 and clean air 10. Under the above numerical conditions, the condensed water 8 is about 0.73cc / ft 3 air was collected. Here, an example of the results of measuring the concentration of suspended particulates before and after the above processing is shown in FIG. Normally, since the bacteria have a size of 0.5 μm or more, it can be understood from the figure that 98% or more of the bacteria are collected in the condensed water 8.
【００２３】次に、上記凝縮水８を１ｌに対し、トリス
緩衝液１１を３ｍｌを加え、反応管１２において温度１
０５℃で５分間加熱抽出を行ない、ＡＴＰが抽出された
凝縮水８を常温まで冷却し、石英製測定キュベット１４
に定量送液する。この測定キュベット１４には、波長２
４９ｎｍを有するＫｒＦレーザ１５から励起光が照射さ
れ、蛍光１７を発光させる。この蛍光１７は、フィルタ
２１によって波長３９０ｎｍのみの蛍光を光電子倍増管
２２に送られ、蛍光量を計測積算し、この蛍光量から菌
数が算出される。Next, 3 ml of Tris buffer 11 was added to 1 liter of the condensed water 8 and the temperature was adjusted to 1 in the reaction tube 12.
Heat extraction is performed at 05 ° C. for 5 minutes, the condensed water 8 from which ATP is extracted is cooled to room temperature, and a quartz measurement cuvette 14 is used.
To a fixed amount. This measurement cuvette 14 has a wavelength of 2
Excitation light is emitted from the KrF laser 15 having a wavelength of 49 nm, and fluorescence 17 is emitted. This fluorescent light 17 is sent to the photomultiplier tube 22 by the filter 21 and only has a wavelength of 390 nm, the fluorescent amount is measured and integrated, and the number of bacteria is calculated from this fluorescent amount.
【００２４】予め菌濃度が既知である凝縮水８を用いて
発光量を計測した場合の相関関係を図３に示す。更に、
この検定曲線を用いて、蛍光量測定値から求めた菌濃度
と、実際の循環エア４に注入した菌濃度の関係を図４に
示す。同図から分かるように、計測値は実際の注入値に
比較し、多少の誤差はあるが実用上は問題のない精度で
ある。尚、上述した空中浮遊細菌の自動計測システムを
応用することにより、清涼飲料等の飲料水中の菌数計測
も可能である。FIG. 3 shows the correlation when the amount of luminescence is measured using the condensed water 8 of which the bacterial concentration is known in advance. Furthermore,
FIG. 4 shows the relationship between the bacterial concentration obtained from the fluorescence amount measurement value and the actual bacterial concentration injected into the circulating air 4 using this calibration curve. As can be seen from the figure, the measured value has a slight error compared with the actual injection value, but the accuracy is practically no problem. It is also possible to measure the number of bacteria in drinking water such as soft drinks by applying the above-mentioned automatic measurement system of airborne bacteria.
【００２５】[0025]
【発明の効果】以上詳記したようにこの発明によれば、
クリーンルームの循環エア中より直接浮遊細菌を補集
し、これよりＡＴＰを抽出・計量するので、抽出時間５
分、蛍光量測定１〜２分の合計計測所用時間が６〜７分
となる。これにより、従来に比べて計測所用時間が短縮
でき、リアルタイムでの評価が可能となる。又、各工程
が機器分析であるため自動化が可能であり、これにより
省力化ができる。As described above in detail, according to the present invention,
Airborne bacteria are directly collected from the circulating air in the clean room, and ATP is extracted and weighed from this, so the extraction time is 5
Minutes, the total measurement time for measuring the amount of fluorescence for 1 to 2 minutes is 6 to 7 minutes. As a result, the time required for measurement can be shortened compared to the conventional method, and real-time evaluation becomes possible. Further, since each step is instrumental analysis, it can be automated, which can save labor.
【００２６】更に、菌数の少ない空気を測定対象とする
場合でも、液滴補集による菌濃度工程や蛍光測定時間の
延長等により十分な測定精度を維持することができ、測
定が循環エア全体を対象にできるため、サンプル間のデ
ータの不一致がなく、上述したように各工程が機器分析
であるため従来の分析者間に生じるデータの不一致をも
解消することができる。Further, even when air having a small number of bacteria is to be measured, sufficient measurement accuracy can be maintained due to the bacteria concentration process by collecting droplets and the extension of the fluorescence measurement time. Since there is no inconsistency in data between samples, and since each step is an instrumental analysis as described above, inconsistency in data that occurs between analysts in the related art can be eliminated.
【図１】この発明の一実施例に係る空中浮遊細菌の自動
計測システムのの構成を示すブロック図。FIG. 1 is a block diagram showing the configuration of an automatic airborne bacteria measurement system according to an embodiment of the present invention.
【図２】同実施例における微粒子除去効果を示すグラ
フ。FIG. 2 is a graph showing the effect of removing fine particles in the same example.
【図３】同実施例における菌濃度と発光量の関係を示す
グラフ。FIG. 3 is a graph showing the relationship between bacterial concentration and luminescence amount in the same example.
【図４】同実施例における注入菌濃度と計測菌濃度の関
係を示すグラフ。FIG. 4 is a graph showing the relationship between the concentration of injected bacteria and the concentration of measured bacteria in the same example.
１…コンプレッサ、２…モータ、３…クリーンルーム、
４…循環エア、５…加湿器、６…タービン、７…モー
タ、８…凝縮水、９…遠心分離器、１０…清浄エア、１
１…沸騰トリス緩衝液、１２…反応管、１３…冷却管、
１４…測定キュベット、１５…ＫｒＦレーザ、１６…レ
ンズ、１７…蛍光、１８…レンズ、１９…スリット、２
０…レンズ、２１…フィルタ、２２…光電子倍増管、２
３…光度計・記録計。1 ... Compressor, 2 ... Motor, 3 ... Clean room,
4 ... Circulating air, 5 ... Humidifier, 6 ... Turbine, 7 ... Motor, 8 ... Condensed water, 9 ... Centrifuge, 10 ... Clean air, 1
1 ... Boiling Tris buffer solution, 12 ... Reaction tube, 13 ... Cooling tube,
14 ... Measurement cuvette, 15 ... KrF laser, 16 ... Lens, 17 ... Fluorescence, 18 ... Lens, 19 ... Slit, 2
0 ... Lens, 21 ... Filter, 22 ... Photomultiplier tube, 2
3 Photometer / recorder.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｆ２４Ｆ 7/06 Ｃ 6925−3Ｌ ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Internal reference number FI technical display location F24F 7/06 C 6925-3L
Claims (1)
に付着させて補集液とする補集手段と、 この補集手段より生成された上記補集液に沸騰トリス緩
衝液を添加して検液を生成する検液生成手段と、 この検液生成手段により生成された検液を励起させる励
起手段と、 この励起手段により発光した蛍光の蛍光量を計測する蛍
光量計測手段と、 この蛍光量計測手段により計測された蛍光量から測定対
象である上記空気中の浮遊細菌を算出する算出手段とを
具備することを特徴とする空中浮遊細菌の自動計測シス
テム。1. A collecting means for adhering airborne bacteria in the air to be measured to droplets as a collecting solution, and a boiling Tris buffer solution added to the collecting solution generated by the collecting means. A test solution generating means for generating a test solution, an exciting means for exciting the test solution generated by the test solution generating means, and a fluorescence amount measuring means for measuring a fluorescence amount of fluorescence emitted by the exciting means, An automatic measurement system of airborne bacteria, comprising: a calculation unit for calculating the above-mentioned airborne bacteria as a measurement target from the fluorescence amount measured by the fluorescence amount measurement unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP563292A JPH05184350A (en) | 1992-01-16 | 1992-01-16 | System for automatic counting of bacterial cell suspending in air |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP563292A JPH05184350A (en) | 1992-01-16 | 1992-01-16 | System for automatic counting of bacterial cell suspending in air |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH05184350A true JPH05184350A (en) | 1993-07-27 |
Family
ID=11616528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP563292A Withdrawn JPH05184350A (en) | 1992-01-16 | 1992-01-16 | System for automatic counting of bacterial cell suspending in air |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH05184350A (en) |
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