JPH05154194A - Continuous measuring instrument for biochemical material - Google Patents
Continuous measuring instrument for biochemical materialInfo
- Publication number
- JPH05154194A JPH05154194A JP3322183A JP32218391A JPH05154194A JP H05154194 A JPH05154194 A JP H05154194A JP 3322183 A JP3322183 A JP 3322183A JP 32218391 A JP32218391 A JP 32218391A JP H05154194 A JPH05154194 A JP H05154194A
- Authority
- JP
- Japan
- Prior art keywords
- sensor
- liquid
- output signal
- concentration
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- External Artificial Organs (AREA)
- Control Of Non-Electrical Variables (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、生化学物質の測定装置
に関し、特に１つの試料の濃度変化を連続的に測定する
装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for measuring biochemical substances, and more particularly to an apparatus for continuously measuring the concentration change of one sample.
【０００２】[0002]
【従来の技術】従来の生化学測定装置で生化学物質の濃
度を測定する場合、測定者は測定対象の生化学物質を含
む試料液をピペットで秤量し、秤量した試料液を反応槽
に注入する。反応槽にはセンサが設置されており、試料
に含まれる生化学物質はそのセンサによって検出され
る。そして、生化学物質の濃度はセンサの出力信号にも
とづいて算出される。2. Description of the Related Art When measuring the concentration of a biochemical substance with a conventional biochemical measuring device, a measurer measures the sample liquid containing the biochemical substance to be measured with a pipette and injects the measured sample liquid into a reaction tank. To do. A sensor is installed in the reaction tank, and the biochemical substance contained in the sample is detected by the sensor. Then, the concentration of the biochemical substance is calculated based on the output signal of the sensor.
【０００３】[0003]
【発明が解決しようとする課題】しかし、このような従
来の生化学測定装置では、測定者が試料を秤量する必要
があるため、生化学物質の濃度変化を充分に短い時間間
隔で連続的に測定することはできなかった。また、セン
サの反応時間が長く、その点でも連続的な測定は不可能
であった。その結果、従来の生化学測定装置では人工透
析における透析液中の尿素濃度の変動や、手術中の患者
の血液中のグルコース濃度の変動を知ることができなか
った。However, in such a conventional biochemical measuring device, since it is necessary for the measurer to weigh the sample, the change in the concentration of the biochemical substance is continuously changed at sufficiently short time intervals. It could not be measured. Moreover, the reaction time of the sensor was long, and continuous measurement was impossible in that respect as well. As a result, the conventional biochemical measuring device could not know the fluctuation of the urea concentration in the dialysate during artificial dialysis and the fluctuation of the glucose concentration in the blood of the patient during surgery.
【０００４】本発明の目的は、このような問題を解決
し、生化学物質の濃度変化を充分に短い時間間隔で連続
的に測定できる生化学物質の連続測定装置を提供するこ
とにある。An object of the present invention is to solve the above problems and to provide a biochemical substance continuous measuring apparatus capable of continuously measuring a change in biochemical substance concentration at sufficiently short time intervals.
【０００５】[0005]
【課題を解決するための手段】本発明は、生化学物質の
濃度を測定する装置において、液体中の生化学物質を検
出するバイオセンサと、このセンサが設置されたセンサ
反応部と、このセンサ反応部に一定量の緩衝液を注入す
る第１の注入装置と、前記センサ反応部に、生化学物質
を含む一定濃度の標準液を、一定量注入する第２の注入
装置と、前記センサ反応部に一定量の試料液を注入する
第３の注入装置と、前記バイオセンサの出力信号が変化
する速度にもとづいて前記試料液の濃度を求める信号処
理部とを備えたことを特徴とする。DISCLOSURE OF THE INVENTION The present invention relates to a device for measuring the concentration of a biochemical substance, a biosensor for detecting a biochemical substance in a liquid, a sensor reaction section in which this sensor is installed, and this sensor. A first injection device for injecting a fixed amount of buffer solution into the reaction part, a second injection device for injecting a fixed amount of a standard solution containing a biochemical substance into the sensor reaction part at a fixed amount, and the sensor reaction A third injection device for injecting a fixed amount of the sample solution into the section, and a signal processing section for obtaining the concentration of the sample solution based on the speed at which the output signal of the biosensor changes.
【０００６】[0006]
【実施例】次に本発明の実施例について図面を参照して
説明する。図１に本発明による生化学物質の連続測定装
置の一例を示す。センサ反応部１は、内部にバイオセン
サ１２が設置されており、センサ１２が反応部１内に注
入された液体に浸る構造となっている。送液装置２は、
シリンジポンプ３を介して液体をｈ方向から吸引し、ｉ
方向に引き込む。そして、引き込んだ液体を秤量し、一
定量の液体をｊ方向に吐出して、チューブ１１を通じて
反応部１に注入する。シリンジポンプ３は、常にｈ→ｉ
→ｊの方向にのみ液体が流れる構造となっている。Embodiments of the present invention will now be described with reference to the drawings. FIG. 1 shows an example of an apparatus for continuously measuring biochemical substances according to the present invention. The sensor reaction unit 1 has a biosensor 12 installed therein, and has a structure in which the sensor 12 is immersed in the liquid injected into the reaction unit 1. The liquid feeding device 2 is
The liquid is sucked from the h direction via the syringe pump 3,
Pull in the direction. Then, the drawn liquid is weighed, a fixed amount of the liquid is discharged in the j direction, and is injected into the reaction part 1 through the tube 11. The syringe pump 3 is always h → i
The structure is such that the liquid flows only in the direction of → j.
【０００７】ボトル８，９にはそれぞれ緩衝液、および
測定対象生化学物質の一定濃度の標準液が蓄積されてい
る。ボトル１０は、測定対象の生化学物質を含む試料液
を蓄積するためのものであり、チューブを通じてｇ方向
より試料液が常に流入している。ボトル７は、反応部１
から排出される液体を蓄積するためのものである。A buffer solution and a standard solution having a constant concentration of the biochemical substance to be measured are accumulated in the bottles 8 and 9, respectively. The bottle 10 is for accumulating a sample solution containing a biochemical substance to be measured, and the sample solution constantly flows in from the g direction through the tube. Bottle 7 is reaction part 1
It is for accumulating liquid discharged from.
【０００８】切換弁４は、ボトル８とシリンジポンプ３
とを結ぶａ−ｂ間の流路と、切換弁５とシリンジポンプ
３とを結ぶａ−ｃ間の流路のいずれかを設定できる。切
換弁５は、ボトル９と切換弁４とを結ぶｄ−ｅ間の流路
と、切換弁４とボトル１０とを結ぶｄ−ｆ間の流路のい
ずれかを設定できる。The switching valve 4 includes a bottle 8 and a syringe pump 3.
It is possible to set either a flow path between a and b that connects to and a flow path between a and c that connects the switching valve 5 and the syringe pump 3. The switching valve 5 can set either a flow path between d and e that connects the bottle 9 and the switching valve 4 or a flow path between d and f that connects the switching valve 4 and the bottle 10.
【０００９】制御部６は、切換弁４，５を制御して流路
を設定し、また送液装置２を制御して一定量の液体が反
応部１に注入されるようにする。また、センサ１２から
信号を受け取って処理し、生化学物質の濃度を算出す
る。算出された濃度は表示部１３に表示される。The control unit 6 controls the switching valves 4 and 5 to set a flow path, and controls the liquid delivery device 2 so that a certain amount of liquid is injected into the reaction unit 1. Further, it receives a signal from the sensor 12 and processes it to calculate the concentration of the biochemical substance. The calculated concentration is displayed on the display unit 13.
【００１０】次に図２のフローチャートを参照して動作
を説明する。制御部６は弁４の流路をａ−ｂ間の流路に
設定し（ステップＳ１）、送液装置２を制御してボトル
８の緩衝液を一定量センサ反応部１に送液してセンサ１
２が緩衝液に浸る状態にする（ステップＳ２）。制御部
６はこのときセンサ１２が出力する信号を取り込み（ス
テップＳ３）、その値を測定対象の生化学物質が含まれ
ない状態の値として記憶する。Next, the operation will be described with reference to the flowchart of FIG. The control unit 6 sets the flow path of the valve 4 to the flow path between a and b (step S1), controls the liquid sending device 2 and sends the constant amount of the buffer solution of the bottle 8 to the sensor reaction section 1. Sensor 1
2 is immersed in the buffer solution (step S2). At this time, the control unit 6 takes in the signal output from the sensor 12 (step S3) and stores the value as a value in a state in which the biochemical substance to be measured is not included.
【００１１】次に、制御部６は弁５の流路をｅ−ｄ間の
流路に設定し、弁４の流路をａ−ｃ間の流路に設定する
（ステップＳ４）。その後、送液装置２に標準液をボト
ル９から吸引させ、その一定量は反応部１に吐出させる
（ステップＳ５）。その結果、緩衝液に標準液が混ざ
り、センサ１２の出力信号レベルは上昇する。制御部６
はそのセンサ１２の出力信号を取り込み（ステップＳ
６）、出力信号の微分値を計算して微分値が最大となっ
たか否かを調べ（ステップＳ７）、微分値が最大値とな
ったときのセンサ出力信号の値を記憶する。これで、測
定対象生化学物質の一定濃度の液体が反応部１に注入さ
れた場合のセンサ１２の出力値が分ったことになる。Next, the controller 6 sets the flow path of the valve 5 to the flow path between ed and the flow path of the valve 4 to the flow path between a and c (step S4). After that, the liquid sending device 2 is caused to suck the standard liquid from the bottle 9, and a certain amount thereof is discharged to the reaction part 1 (step S5). As a result, the standard solution is mixed with the buffer solution, and the output signal level of the sensor 12 rises. Control unit 6
Captures the output signal of the sensor 12 (step S
6) The differential value of the output signal is calculated to check whether the differential value is maximum (step S7), and the value of the sensor output signal when the differential value is maximum is stored. This means that the output value of the sensor 12 when the liquid having a constant concentration of the biochemical substance to be measured is injected into the reaction section 1 is known.
【００１２】次に、制御部６は弁４の流路をａ−ｂ間の
流路に設定し（ステップＳ８）、緩衝液を反応部１に送
液してセンサ１２を洗浄し（ステップＳ９）、次の測定
の準備を行う。その後、弁５，４の流路をそれぞれｄ−
ｆ間およびａ−ｃ間の流路に設定する（ステップＳ１
０）。そして、制御部６は送液装置２に、一定量の試料
液をボトル１０から反応部１に送液させる（ステップＳ
１１）。その結果、反応部１内の緩衝液と試料液とが混
ざり、センサ１２の出力信号レベルは上昇する。制御部
６はこのセンサ出力信号を取り込み（ステップＳ１
２）、標準液の測定の場合と同様に、センサ出力信号の
微分値を計算し、微分値が最大となったか否かを調べ
（ステップＳ１３）、微分値が最大となったときの出力
信号の値を取得する。そして、その値と、標準液測定時
の値とを比較して試料液の濃度を求め、得られた濃度を
表示部１３に表示する。Next, the control unit 6 sets the flow path of the valve 4 to the flow path between a and b (step S8), sends the buffer solution to the reaction section 1 and cleans the sensor 12 (step S9). ), Prepare for the next measurement. After that, the flow paths of the valves 5 and 4 are respectively d-
It is set to the flow path between f and a-c (step S1).
0). Then, the control unit 6 causes the liquid feeding device 2 to feed a fixed amount of the sample liquid from the bottle 10 to the reaction unit 1 (step S
11). As a result, the buffer solution in the reaction section 1 and the sample solution are mixed, and the output signal level of the sensor 12 rises. The control unit 6 fetches this sensor output signal (step S1
2) As in the case of the measurement of the standard solution, the differential value of the sensor output signal is calculated, and it is checked whether or not the differential value is the maximum (step S13), and the output signal when the differential value is the maximum. Get the value of. Then, the value is compared with the value at the time of measuring the standard solution to obtain the concentration of the sample liquid, and the obtained concentration is displayed on the display unit 13.
【００１３】その後、制御部６は弁４の流路をａ−ｂ間
の流路に設定し（ステップＳ１５）、緩衝液を反応部１
に送液してセンサ１２を洗浄する（ステップＳ１６）。Thereafter, the control unit 6 sets the flow path of the valve 4 to the flow path between a and b (step S15), and the buffer solution is added to the reaction unit 1.
And the sensor 12 is washed (step S16).
【００１４】以降、制御部６は、試料液をボトル１０か
ら反応部１に送液して濃度を測定する動作を所定の時間
間隔で繰り返し、そのつど得られる濃度を表示部１３に
表示する（ステップＳ１０〜ステップＳ１６）。測定者
はその表示を見ることによって、測定対象の生化学物質
の濃度変化を知ることができる。Thereafter, the control unit 6 repeats the operation of feeding the sample liquid from the bottle 10 to the reaction unit 1 and measuring the concentration at predetermined time intervals, and displays the concentration obtained on each occasion on the display unit 13 ( Steps S10 to S16). By looking at the display, the measurer can know the change in the concentration of the biochemical substance to be measured.
【００１５】[0015]
【発明の効果】以上説明したように本発明の生化学物質
の連続測定装置では、測定対象の生化学物質を含む試料
液は、第３の注入装置によって一定量が反応部に自動的
に注入される。また、試料液の濃度は信号処理部によ
り、バイオセンサの出力信号の変化速度にもとづいて求
められるので、センサの反応時間が長くても、短時間で
濃度が測定される。従って、充分に短い時間間隔で濃度
を測定することができ、濃度変化を連続的に測定するこ
とが可能となる。その結果、人工透析における透析液中
の尿素濃度の変動や、手術中の患者の血液中のグルコー
ス濃度の変動を知ることが可能となる。As described above, in the continuous biochemical substance measuring apparatus of the present invention, a fixed amount of the sample liquid containing the biochemical substance to be measured is automatically injected into the reaction section by the third injection device. To be done. Further, since the concentration of the sample liquid is obtained by the signal processing unit based on the changing speed of the output signal of the biosensor, even if the reaction time of the sensor is long, the concentration can be measured in a short time. Therefore, the concentration can be measured at sufficiently short time intervals, and the change in concentration can be continuously measured. As a result, it becomes possible to know the fluctuation of the urea concentration in the dialysate during artificial dialysis and the fluctuation of the glucose concentration in the blood of the patient under operation.
【図１】本発明による生化学物質の連続測定装置の一例
を示すブロック図である。FIG. 1 is a block diagram showing an example of an apparatus for continuously measuring biochemical substances according to the present invention.
【図２】図１の生化学物質の連続測定装置の動作を説明
するためのフローチャートである。FIG. 2 is a flow chart for explaining the operation of the biochemical substance continuous measurement apparatus of FIG.
１ センサ反応部 ２ 送液装置 ３ シリンジポンプ ４，５ 切換弁 ６ 制御部 ７〜１０ ボトル １１ チューブ １２ バイオセンサ １３ 表示部 DESCRIPTION OF SYMBOLS 1 Sensor reaction part 2 Liquid feeding device 3 Syringe pump 4,5 Switching valve 6 Control part 7-10 Bottle 11 Tube 12 Biosensor 13 Display part
Claims (3)
て、 液体中の生化学物質を検出するバイオセンサと、 このセンサが設置されたセンサ反応部と、 このセンサ反応部に一定量の緩衝液を注入する第１の注
入装置と、 前記センサ反応部に、生化学物質を含む一定濃度の標準
液を、一定量注入する第２の注入装置と、 前記センサ反応部に一定量の試料液を注入する第３の注
入装置と、 前記バイオセンサの出力信号が変化する速度にもとづい
て前記試料液の濃度を求める信号処理部とを備えたこと
を特徴とする生化学物質の連続測定装置。1. A device for measuring the concentration of a biochemical substance, a biosensor for detecting a biochemical substance in a liquid, a sensor reaction part in which the sensor is installed, and a fixed amount of buffer solution in the sensor reaction part. A second injection device for injecting a fixed amount of a standard solution containing a biochemical substance into the sensor reaction section at a fixed amount, and a fixed amount of sample solution to the sensor reaction section. A continuous measuring device for biochemical substances, comprising: a third injecting device for injecting; and a signal processing unit for obtaining the concentration of the sample solution based on the speed at which the output signal of the biosensor changes.
変化速度が最大となったときの前記センサ出力信号の値
より、前記試料液の濃度を求めることを特徴とする請求
項１記載の生化学物質の連続測定装置。2. The signal processing unit obtains the concentration of the sample liquid from the value of the sensor output signal when the rate of change of the sensor output signal is maximized. Continuous measuring device for biochemical substances.
と、これら第１および第２の注入口より注入された液体
を排出する排出口とを備えた第１の弁と、 この弁を制御する第１の制御部とを備え、 前記第２の注入装置は、 前記標準液が注入される第１の注入口と、第２の注入口
と、これら第１および第２の注入口より注入された液体
を排出する排出口とを備えた第２の弁と、 この弁を制御する第２の制御部とを備え、 前記第３の注入装置は、前記第２の弁を含み、その前記
第２の注入口に前記試料液が注入されることを特徴とす
る請求項１記載の生化学物質の連続測定装置。3. The first injection device comprises a first injection port into which the buffer solution is injected, a second injection port, and the liquid injected from the first and second injection ports. A first valve having a discharge port for controlling the valve, and a first control unit for controlling the valve, the second injection device having a first injection port for injecting the standard solution, A second valve having two inlets and an outlet for discharging the liquid injected from the first and second inlets; and a second controller for controlling the valve, The continuous measuring device for biochemical substances according to claim 1, wherein the third injection device includes the second valve, and the sample solution is injected into the second injection port.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3322183A JPH05154194A (en) | 1991-12-06 | 1991-12-06 | Continuous measuring instrument for biochemical material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3322183A JPH05154194A (en) | 1991-12-06 | 1991-12-06 | Continuous measuring instrument for biochemical material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH05154194A true JPH05154194A (en) | 1993-06-22 |
Family
ID=18140875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3322183A Pending JPH05154194A (en) | 1991-12-06 | 1991-12-06 | Continuous measuring instrument for biochemical material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH05154194A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102115907B1 (en) * | 2018-12-24 | 2020-05-27 | 주식회사 조인트리 | Sequential Injection Analysis System for Nitrogen Fertilization Monitoring in Inland Aquaculture |
-
1991
- 1991-12-06 JP JP3322183A patent/JPH05154194A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102115907B1 (en) * | 2018-12-24 | 2020-05-27 | 주식회사 조인트리 | Sequential Injection Analysis System for Nitrogen Fertilization Monitoring in Inland Aquaculture |
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