JPH05153971A - Agent for suppressing or deodorizing ill odor of feces and method for suppressing or deodorizing ill odor of feces - Google Patents
Agent for suppressing or deodorizing ill odor of feces and method for suppressing or deodorizing ill odor of fecesInfo
- Publication number
- JPH05153971A JPH05153971A JP3317831A JP31783191A JPH05153971A JP H05153971 A JPH05153971 A JP H05153971A JP 3317831 A JP3317831 A JP 3317831A JP 31783191 A JP31783191 A JP 31783191A JP H05153971 A JPH05153971 A JP H05153971A
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- JP
- Japan
- Prior art keywords
- suppressing
- feces
- deodorizing
- agent
- odor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Treating Waste Gases (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、糞尿の悪臭発生抑制乃
至悪臭の脱臭剤及びそれらを用いた糞尿の悪臭発生抑制
乃至悪臭の脱臭方法に関し、より詳しくは、養豚、養牛
等の畜産における糞尿の悪臭発生防止並びに脱臭、家庭
用及び業務用便所の人の糞尿の悪臭発生防止並びに脱臭
に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an offensive odor suppressing agent for manure or a deodorant for malodor, and a method for suppressing offensive odor generating of manure or deodorizing a malodor using the same, and more specifically, in livestock farming such as pig raising and cattle raising Prevention of offensive odor of manure and deodorization, prevention of offensive odor of manure and deodorization of people in domestic and commercial toilets.
【０００２】[0002]
【従来の技術とその問題点】従来、人や動物の***物で
ある糞尿は、有機肥料としての価値を有しながら、特有
の悪臭を放つこと、取扱いが煩雑であること、衛生上問
題があることなどから、今日では肥料として扱われるこ
とはほとんど無い。従って糞尿は、廃尿として処理され
るか、一部メタン発酵されて廃棄されている。しかしな
がら、これらの方法は、大規模な設備を必要とし、処理
に要する費用が高くなるので、特別な設備を必要としな
い実用的な方法の実現が切望されている。2. Description of the Related Art Conventionally, manure, which is excrement of humans and animals, has a value as an organic fertilizer, but emits a peculiar odor, is complicated to handle, and has a hygiene problem. For that reason, it is rarely treated as fertilizer today. Therefore, the manure is treated as waste urine or is partially methane-fermented and discarded. However, since these methods require large-scale equipment and increase the cost required for treatment, realization of a practical method that does not require special equipment is desired.
【０００３】一方、ヒトも含め、種々の動物の糞尿から
放たれる悪臭は、重大な環境問題となっている。そのた
め、蓄積される糞尿の悪臭による環境悪化を防止するた
めに、以下のような人の糞尿に対する脱臭剤が開発され
ているが、今だ夫々欠点がある。On the other hand, the malodor emitted from manure of various animals including humans has become a serious environmental problem. Therefore, the following deodorants for human excrement have been developed in order to prevent environmental deterioration due to the bad odor of accumulated excrement, but there are still drawbacks.
【０００４】[0004]
【表１】 [Table 1]
【０００５】それらに変わる糞尿の脱臭方法として、微
生物を用いる脱臭方法が研究され始めてきた。例えば、
豚糞、牛糞に対して糸状菌（発酵工学会誌、５４巻、３
３３頁（１９７６年））、放線菌［豚糞に対しての報
告、アプライド エンビロンメンタル マイクロバイオ
ロジー(Appl. Environ. Microbiol.) 、３６巻、４８７
頁（１９７８年）、鶏糞に対しての報告、発酵工学会
誌、５８巻、１９７頁（１９８０年）］、鶏糞に対して
酵母（発酵工学会誌、５５巻、１３４頁（１９７７
年））の報告がされているが、それらは特定の悪臭成分
にしか有効でなく、悪臭を完全に抑えるものではない。
悪臭防止剤としては、あらゆる動物の糞尿に広く適用で
きるものが望まれているが、一般に、糞尿を***する動
物が異なると、糞尿中に存在する微生物の相違、悪臭発
生物質の相違（ＰＰＭ、１９７４年、１０巻、２８−３
７頁）、糞尿中の物質の相違、置かれている状況の相違
などから異なり、動物により脱臭剤を変えることが行わ
れているのが実情である。As an alternative deodorizing method for excrement of manure, research on a deodorizing method using microorganisms has begun. For example,
Filamentous fungus against pig manure and cow manure (Fermentation Engineering Society, 54, 3
33 (1976)), actinomycetes [report on swine manure, Applied Environmental Microbiology, Appl. Environ. Microbiol., 36, 487]
P. (1978), reports on poultry manure, Journal of Fermentation Engineering, Vol. 58, p. 197 (1980)], Yeast for poultry manure (Fermentation Engineering, Vol. 55, p. 134 (1977).
However, they are effective only for specific malodorous components and do not completely suppress malodors.
As the malodor preventing agent, one that can be widely applied to the feces of all animals is desired, but in general, when the animals excreting the excrement are different, the difference in the microorganisms present in the excrement and the difference in the malodor generating substance (PPM, 1974, 10 volumes, 28-3
(P. 7), the deodorant is changed depending on the animal because of the difference in the substance in the excrement and the difference in the condition in which it is placed.
【０００６】本発明者らは、これまでほとんど報告のな
かった細菌種の中から、鶏糞の脱臭に有効な菌を分離し
た（科学と工業、６３巻、９号、３７４頁（１９８９
年））。この菌は発酵鶏糞から分離され、本発明者によ
り同定した結果、この菌はバチルス・メガテリウム（Ba
cillus megaterium ）の１株であると考えられ、バチル
ス・メガテリウムＢ１（B acillus mega terium B1）と名
付けられた。[0006] The present inventors isolated a fungus effective for deodorizing chicken manure from bacterial species that had hardly been reported so far (Science and Industry, 63, 9, 374 (1989).
Year)). This bacterium was isolated from fermented chicken manure, and as a result of identification by the present inventors, this bacterium was identified as Bacillus megaterium ( Ba
is considered to be one (1) share of cillus megaterium), it was named Bacillus megaterium B1 (B acillus mega terium B1) .
【０００７】現在、哺乳動物の糞尿、例えば養豚、養牛
の糞尿、人の糞尿等に対して、広く効果的に悪臭成分の
発生を抑制する微生物製剤並びにそれを用いる方法は、
知られておらず、有効な薬剤が切望されている。[0007] At present, a microbial preparation which suppresses the generation of malodorous components in mammals, such as pig farming, cattle farming, human manure, etc., and a method of using the same are as follows:
An unknown and effective drug is craved.
【０００８】[0008]
【発明が解決しようとする課題】本発明の目的は、哺乳
類の糞尿の種々の悪臭成分の発生を長期間抑制する悪臭
発生抑制乃至脱臭剤及びそれらを哺乳類の糞尿に添加す
ることにより用いる悪臭発生抑制乃至脱臭方法を提供す
ることにある。DISCLOSURE OF THE INVENTION The object of the present invention is to suppress the generation of various malodorous components of mammalian excrement for a long period of time, and to suppress the generation of malodorous deodorants and the generation of malodorous substances by adding them to mammalian excrement. An object is to provide a method for suppressing or deodorizing.
【０００９】[0009]
【課題を解決するための手段】本発明者は、上記目的を
達成するために更に鋭意研究を行った結果、発酵鶏糞由
来のバチルス・メガテリウムＢ１（Bacillus megateriu
m B1）が、哺乳類の糞尿への添加により、１）悪臭成分
を無臭成分に変化させ、２）雑菌による悪臭の発生を抑
制し、更に、３）その効果は長時間持続されるなどの効
果を有することを見出し、本発明を完成した。The present inventors SUMMARY OF THE INVENTION As a result of further intense study to achieve the above object, fermented chicken manure from Bacillus megaterium B1 (Bacillus megateriu
m B1) is added to mammalian excrement, 1) changes malodorous components to odorless components, 2) suppresses the generation of malodors due to various bacteria, and 3) its effect is maintained for a long time. The present invention has been completed by finding out that
【００１０】即ち、本発明は哺乳類の悪臭発生抑制乃至
悪臭の脱臭に当たり、バチルス・メガテリウム（Bacill
us megaterium ）種に属する細菌を有効成分とする悪臭
発生抑制乃至脱臭剤に関する。[0010] That is, the present invention relates to Bacillus megaterium ( Bacill) for suppressing the generation of malodor in mammals and deodorizing malodor.
us megaterium ). The present invention relates to a deodorant which suppresses the generation of bad odors, which contains a bacterium belonging to the species as an active ingredient.
【００１１】本発明において、用いられる細菌は、バチ
ルス・メガテリウム（Bacillus meg aterium ）種に属す
る細菌であればいずれのものも使用できる。好ましく
は、例えば、本発明者がコンポスト化した発酵鶏糞から
分離したバチルス・メガテリウムＢ１（Bacillus megat
erium B1）（以下単にＢ１株と略する。）を挙げること
ができる。このＢ１株は、上述のように本発明者が見出
した菌株であり、工業技術院微生物工業技術研究所（微
工研）に寄託されている（微工研菌寄第１２３９４号(F
ERM P-12394)）。この菌株の菌学的性質は、以下の通り
である。[0011] In the present invention, the bacteria used can be used any one as long as bacteria belonging to Bacillus megaterium (Bacillus meg aterium) species. Preferably, for example, Bacillus megaterium B1 ( Bacillus megatium B1 isolated from fermented chicken manure composted by the present inventor
erium B1) (hereinafter simply abbreviated as B1 strain). This B1 strain is a strain found by the present inventor as described above, and has been deposited at the Institute of Microbial Technology of Japan (AIST) (Ministry of Industrial Research No. 12394 (F).
ERM P-12394)). The mycological properties of this strain are as follows.
【００１２】（ａ）形態 培地組成が肉汁及び肉汁寒天である、液体培地及び寒天
培地に生育した細胞について調べた。(A) Morphology The cells grown on the liquid medium and the agar medium having the medium composition of broth and broth agar were examined.
【００１３】細胞の形及び大きさ：桿菌、1.0 〜1.3 ×
1.8〜4.0 μｍ 細胞の多形性 ： 無 運動性の有無 ： 有 胞子の有無 ： 有 胞子嚢の形：楕円、胞子の部位：先端部 グラム染色性 ： 陽性 抗酸性 ： 無 （ｂ）各培地における生育状態 肉汁寒天平板培養 生育：良好、コロニーの形状：拡幅状、平面の形状：平
滑、透明度：不透明 肉汁寒天斜面培養 断面の***状態：薄膜状、質：バター状、光沢：湿光、
色調：淡乳白色 肉汁液体培養 表面の発育：無、濁度：中程度、沈澱：無 肉汁ゼラチン穿刺培養 ゼラチンを液化せず、生育：糸状 リトマス・ミルク 凝固せず、ペプトン化せず、色調不変 （ｃ）生理学的性質 硝酸塩の還元 ：陽性 脱窒反応 ：陰性 ＭＲテスト ：陽性 ＶＰテスト ：陰性 インドールの生成 ：陰性 硫化水素の生成 ：陰性 デンプンの加水分解 ：陰性 クエン酸の利用 ：陽性 （Koser の培地とChristensenの培地の併用） 無機窒素源の利用 ： 有 色素の生成 ： 無 ウレアーゼ ：陰性 オキシダーゼ ：陰性 カタラーゼ ：活性陽性 生育の範囲 ：３８℃（１５〜４２℃
で生育可能） 最適ｐＨ８．０（ｐＨ６〜１０で生育可能） 酸素に対する態度 ：好気性 Ｏ−Ｆテスト ：好気的酸生成 酸の生成 ：キシロース、グルコー
ス、ガラクトース、マンノースより生成 グリセリン、マンニトール、アラビノース、果糖、麦芽
糖、乳糖、ショ糖、トレハロースラフィノース、澱粉か
らは生成されず ガスの生成 ：無 この菌株を、バージェーズ マニュアル オブ システ
マティック バクテリオロジー（（Bergey´s Manual o
f Systematic Bacteriology ）、２巻、セクション１
３、ピー．エッチ．エー．スニースら（P.H.A.Sneath e
t al）、ウィリアムズ アンド ウィルキンズ（Willia
ms ＆ Wilkins）、バルチモア（Baltimore ）、１９８
６年）及びバージェーズマニュアル オブ デターミネ
イティブバクテリオロジー（（Bergey´s Manual of De
terminative Bacteriology）、第８版、パート１５、ア
ール．イー．ブッチャナンら（R.E.Buchanan et al）、
ザ ウィリアムズ アンド ウィルキンズ カンパニー
（The Williams ＆ Wilkins Company）、バルチモア
（Baltimore ）、１９７４年）に基づいて調べた結果、
この菌株は、グラム陽性の好気的桿菌で、細胞の径が１
μｍ以上で細胞内胞子を有し、カタラーゼ活性及び硝酸
塩還元活性があり、キシロースを発酵して酸を生成する
ことから、バチルス・メガテリウム（Bacillus megater
ium ）種の一菌株であると推定されたが、細胞内での胞
子の位置及びアラビノースとマンニトールからの酸の生
成の点で、公知の菌株とは異なり、バチルス・メガテリ
ウム（Bacillus megaterium ）の新変種であるとした。Cell shape and size: Bacillus, 1.0-1.3 ×
1.8-4.0 μm Cell polymorphism: Motile / non-motile: Presence of spores: Sporangium shape: Ellipse, spore site: Tip Gram stainability: Positive anti-acidity: None (b) in each medium Growth condition Meat agar plate culture Growth: Good, colony shape: widening, plane shape: smooth, transparency: opaque Meat agar slope culture Cross-section uplifting: thin film, quality: buttery, gloss: wet light,
Color: Light milky white broth liquid culture Surface growth: None, Turbidity: Medium, Precipitation: No broth Gelatin puncture culture No gelatin liquefaction, Growth: Filamentous litmus milk No coagulation, no peptone, no change in color tone ( c) Physiological properties Nitrate reduction: Positive Denitrification reaction: Negative MR test: Positive VP test: Negative Indole formation: Negative Hydrogen sulfide formation: Negative Starch hydrolysis: Negative Utilization of citric acid: Positive (Koser's medium Use of Inorganic Nitrogen Source: Formation of pigment: Urease: Negative oxidase: Negative catalase: Activity positive Growth range: 38 ℃ (15-42 ℃)
Optimum pH 8.0 (It can grow at pH 6 to 10) Attitude toward oxygen: Aerobic OF test: Aerobic acid production Acid production: Production from xylose, glucose, galactose, mannose Glycerin, mannitol, arabinose , Fructose, maltose, lactose, sucrose, trehalose raffinose, and starch, but no gas production: None This strain was used for the Burgers' Manual of Systematic Bacteriology ((Bergey's Manual
f Systematic Bacteriology), Volume 2, Section 1
3, pee. Etch. A. Sneath et al. (PHASneath e
t al), Williams and Wilkins (Willia
ms & Wilkins), Baltimore, 198
6 years) and the Burgers' Manual of Detergent Native Bacteriology ((Bergey's Manual of De
terminative Bacteriology), 8th Edition, Part 15, Earl. E. REBuchanan et al,
As a result of an examination based on The Williams & Wilkins Company (Baltimore, 1974),
This strain is a Gram-positive aerobic bacillus with a cell diameter of 1
Bacillus megaterium ( Bacillus megaterium) has intracellular spores above μm, has catalase activity and nitrate reduction activity, and ferments xylose to produce acid.
ium) was estimated to be species one strain in terms of generation of acid from the position and arabinose and mannitol spores in cells, unlike known strains, new Bacillus megaterium (Bacillus megaterium) It was supposed to be a variant.
【００１４】上記バチルス・メガテリウム種に属する細
菌の培養は、バチルス属に属する公知の細菌と同様に行
うことができる。例えば、廃糖蜜６％、コーンスティー
プ・リカー３％、大豆粕５％を含有する液体培地（pH
7.0）中にて、３７℃で２日間振盪培養することで得ら
れる。The bacterium belonging to Bacillus megaterium species can be cultured in the same manner as the known bacterium belonging to the genus Bacillus. For example, liquid medium containing 6% molasses, 3% corn steep liquor, 5% soybean meal (pH
It can be obtained by shaking culture in 7.0) at 37 ° C. for 2 days.
【００１５】バチルス・メガテリウム種に属する細菌を
有効成分とする糞尿の悪臭発生抑制乃至脱臭剤を製造す
るためには、まず、例えば、上記培養液を遠心分離し、
培養した菌体を集める。製剤の形態はどのようなもので
もよく、例えば、液状、粉末状、顆粒状、錠剤状などの
形が挙げられる。菌体を乾燥させずに使用するときは、
菌体の懸濁液状或いはペースト状などの状態で使用す
る。乾燥菌体として使用するときは、その菌体を噴霧乾
燥、凍結乾燥、真空乾燥などの方法によって乾燥し、粉
末状、顆粒状或いは錠剤状などの状態で使用する。いず
れの場合も添加剤は使用してもしなくても良い。添加剤
としては、公知のものがいずれでも使用できる。例え
ば、水に可溶性のものとしては、糖質、蛋白質、塩類等
が使用され、水に不溶性のものとしては、澱粉、ふすま
（小麦を粉にする時にできる皮のくず）、おがくず、バ
ガス等の澱粉、セルロース、キチン等を成分とする天然
の材料、活性炭、白砂、硅藻土、ガラス等の無機材料、
ポリアクリルアミド、塩化ビニル、ナイロン、ウレタ
ン、ポリエステル等の合成高分子材料を挙げることがで
きる。In order to produce a deodorizing agent for suppressing offensive odor of feces and urine containing a bacterium belonging to the genus Bacillus megaterium as an active ingredient, first, for example, the above culture solution is centrifuged,
Collect the cultured cells. The formulation may be in any form, and examples thereof include liquid, powder, granule, and tablet forms. When using without drying the cells,
It is used in the form of suspension or paste of bacterial cells. When used as dry cells, the cells are dried by a method such as spray-drying, freeze-drying or vacuum-drying, and used in the form of powder, granules or tablets. In any case, the additive may or may not be used. Any known additive can be used as the additive. For example, water-soluble substances include sugars, proteins, salts and the like, and water-insoluble substances include starch, bran (scraps produced when wheat is powdered), sawdust, bagasse, etc. Natural materials such as starch, cellulose, chitin, etc., inorganic materials such as activated carbon, white sand, diatomaceous earth, glass, etc.
Examples thereof include synthetic polymer materials such as polyacrylamide, vinyl chloride, nylon, urethane and polyester.
【００１６】適用する哺乳類の糞尿は、限定されること
なく、いずれの哺乳類のものであっても良い。例えば、
人、豚、牛、馬、羊等の糞尿を挙げることができる。好
ましくは、人の糞尿を挙げることができる。The mammalian excrement to be applied is not limited and may be of any mammal. For example,
The excrement of humans, pigs, cows, horses, sheep, etc. can be mentioned. Preferable one is human excrement.
【００１７】本発明の他の態様は、哺乳類の悪臭発生抑
制乃至悪臭の脱臭に当たり、上記製剤を用いる悪臭発生
抑制乃至脱臭法に関する。[0017] Another aspect of the present invention relates to a method for suppressing the generation of malodor or for deodorizing mammals using the above-mentioned preparation for suppressing the generation of malodor or deodorizing malodor.
【００１８】本発明の製剤の使用方法は、いずれの方法
でも良く、例えば、乾燥菌剤或いは他の形状の菌剤を水
道水に懸濁して流しても良いし、乾燥菌剤或いは他の形
状の菌剤を悪臭発生源に直接振り掛けても良い。The method of using the preparation of the present invention may be any method, for example, a dry fungus agent or a fungus agent in another form may be suspended in tap water and poured, or a dry fungus agent or another form may be used. You may sprinkle the fungus agent of the above directly on the source of the bad smell.
【００１９】製剤の添加量は、対象によって異なるが、
悪臭発生の比較的少ない物に対しては、固形物（糞尿の
乾燥重量）１ｇにつき、１０6 〜１０7 個程度、悪臭発
生の著しいものについては、１０8 〜１０9 個程度の菌
体を添加するのが効果的である。The addition amount of the preparation depends on the subject,
About 10 6 to 10 7 cells per 1 g of solid matter (dry weight of feces and urine) is used for those with a relatively small amount of bad odor, and about 10 8 to 10 9 cells is used for those with a significant amount of bad odor. It is effective to add.
【００２０】本発明の糞尿の悪臭発生抑制乃至脱臭効果
は、本発明の菌が悪臭成分を変換し、この変換によって
できた物質が他の菌によって分解されず、他の菌の生育
を抑えることによって、達成されると推測される。The effect of suppressing or deodorizing offensive odor of feces and urine of the present invention is that the bacterium of the present invention converts a malodorous component, and the substance produced by this conversion is not decomposed by other bacteria, and the growth of other bacteria is suppressed. Is supposed to be achieved by.
【００２１】[0021]
【発明の効果】本発明によれば、以下の様な哺乳類の糞
尿の悪臭除去及び悪臭発生抑制の効果が達成される。EFFECTS OF THE INVENTION According to the present invention, the following effects of removing offensive odors from mammalian excrement and suppressing offensive odors are achieved.
【００２２】（１）本発明品を用いることにより、悪臭
防止のための特別な設備を必要とせずに、悪臭発生防止
を行う事ができる。(1) By using the product of the present invention, it is possible to prevent the generation of offensive odors without requiring special equipment for preventing offensive odors.
【００２３】（２）本発明の細菌は、哺乳動物の糞尿の
広い範囲の悪臭成分の除去及び発生抑制に有効であり、
各種の悪臭発生物を対象とすることが期待できる。(2) The bacterium of the present invention is effective in removing and suppressing the generation of malodorous components in a wide range of mammalian excreta,
It can be expected to target various types of offensive odor products.
【００２４】（３）本発明の細菌は、生育に特別な栄養
物を必要とせず、各種の悪臭発生物中で増殖することが
できるので。作用の効果が持続する。(3) The bacterium of the present invention does not require any special nutrient for growth and can grow in various malodorous substances. The effect of action lasts.
【００２５】（４）本発明の細菌は、悪臭を除去するだ
けでなく、雑菌の繁殖によって悪臭が発生することを抑
制することができる。(4) The bacterium of the present invention can not only remove the malodor, but also suppress the generation of malodor due to the propagation of various bacteria.
【００２６】[0026]
【実施例】以下に実施例を挙げ、本発明をより一層明瞭
なものとする。EXAMPLES The present invention will be further clarified by the following examples.
【００２７】実施例１ Ｂ１株の培養及びＢ１株を含有
する製剤の調製 Ｂ１株を、廃糖蜜６％、コーンスティープ・リカー３
％、大豆粕熱水抽出物５％を含有する液体培地（pH 7.
0）中にて、３７℃で２日間振盪培養した。得られた菌
体を遠心分離によって集め、３％スキムミルク、５％乳
糖、１％グルタミン酸ナトリウムを含む保護剤（pH 7.
0）を加えて凍結乾燥し、粉末の菌剤を調製した。下記
実施例において、この粉末菌剤（１ｇ当たりの生菌数が
約１０11個）を用いて各種糞尿の脱臭と悪臭発生防止を
行った。悪臭発生の判定は、官能試験によって行った。Example 1 Cultivation of B1 strain and preparation of preparation containing B1 strain B1 strain was prepared by mixing molasses 6% with corn steep liquor 3
%, Soybean meal hot water extract 5% liquid medium (pH 7.
Culture was carried out in 0) at 37 ° C. for 2 days with shaking. The obtained bacterial cells were collected by centrifugation, and a protective agent containing 3% skim milk, 5% lactose, 1% sodium glutamate (pH 7.
0) was added and freeze-dried to prepare a powdery fungal agent. In the following examples, the powdered bactericidal agent (the number of viable bacteria per 1 g was about 10 11 ) was used to deodorize various types of manure and prevent the generation of malodor. The sensory test was used to determine the occurrence of malodor.
【００２８】官能試験は、パネラー５人により行い、５
人中４人が悪臭を認めなくなった時点を悪臭消滅時点と
した。The sensory test was conducted by 5 panelists, and 5
The time when 4 of the humans no longer recognized the bad smell was defined as the time when the bad smell disappeared.
【００２９】実施例２ 家庭用水洗便所の容量６０Ｌの簡易浄化槽が、蓄積した
固形物で機能が低下し、マンホール周辺に悪臭を発する
ようになった時期に、実施例１で得られた乾燥Ｂ１菌剤
１０ｇを約１Ｌの水道水に懸濁して、水洗便所より槽中
に流したところ、１時間程度で悪臭が発生しなくなり、
１か月以上悪臭の発生が抑えられた。Example 2 Dry B1 obtained in Example 1 at the time when a simple septic tank with a capacity of 60 L in a domestic flush toilet began to emit a bad odor around the manhole due to deterioration of its function due to accumulated solids. Suspending 10 g of the fungal agent in about 1 L of tap water and pouring it into the tank from the flush toilet, the bad odor disappeared in about 1 hour,
Generation of offensive odor was suppressed for more than one month.
【００３０】実施例３ 汲み取り式便所の便壷が約５０％ほど人の糞尿で満たさ
れた時期に、表面に乾燥菌剤５０ｇを均一にふりかけた
ところ、便壷がほぼ満杯になるまで悪臭の発生が抑えら
れた。Example 3 When about 50% of the toilet bowl of the drawing toilet was filled with human feces and urine, 50 g of the dry fungus agent was evenly sprinkled on the surface of the toilet bowl. Occurrence was suppressed.
【００３１】実施例４ 新鮮牛糞５ｋｇにふすま５００ｇと乾燥菌剤５０ｇを加
えてよく混合し、蓋付プラスチック容器に入れて３０℃
にて３日間保存したところ、牛糞にふすまのみを添加し
たものでは悪臭の発生を抑制できなかったが、菌剤を添
加したものでは３か月以上にわたって悪臭の発生が認め
られなかった。Example 4 To 5 kg of fresh cow dung, 500 g of bran and 50 g of dry fungus agent were added and mixed well, placed in a plastic container with a lid and kept at 30 ° C.
When stored for 3 days in, the generation of malodor could not be suppressed by adding only bran to cow dung, but the generation of malodor was not recognized over 3 months by adding fungal agents.
【００３２】実施例５ 新鮮豚糞５ｋｇにふすま５００ｇと乾燥菌剤５０ｇを加
えてよく混合し、蓋付プラスチック容器に入れて３０℃
にて７日間保存したところ、豚糞にふすまのみを添加し
たものでは悪臭の発生を抑制できなかったが、菌剤を添
加したものでは３か月以上にわたって悪臭の発生が認め
られなかった。Example 5 To 5 kg of fresh pig manure, 500 g of bran and 50 g of dry fungus agent were added and mixed well, put in a plastic container with a lid and kept at 30 ° C.
When stored for 7 days in, the generation of malodor could not be suppressed by adding only bran to pig feces, but the generation of malodor was not observed over 3 months in the case of adding fungal agents.
【００３３】上記実施例から、本発明の製剤を添加する
ことにより、哺乳類の糞尿の悪臭を脱臭し、悪臭の発生
を防止することが確認された。From the above examples, it was confirmed that the addition of the formulation of the present invention deodorizes the offensive odor of the feces and urine of mammals and prevents the offensive odor from occurring.
Claims (4)
aterium ）種に属する細菌を有効成分とする哺乳類の糞
尿の悪臭発生抑制乃至脱臭剤。1. A Bacillus meg
aterium ) is a deodorant for suppressing the offensive odor of mammalian excrement, which contains as an active ingredient bacteria belonging to the species.
aterium ）種に属する細菌が、バチルス・メガテリウム
Ｂ１（Bacillus megaterium B1）であることを特徴と
する請求項１に記載の悪臭発生抑制乃至脱臭剤。2. Bacillus megium
A bacterium belonging to the species Aterium ) is Bacillus megaterium B1 ( Bacillus megaterium B1).
aterium ）種に属する細菌を有効成分とする製剤を用い
る哺乳類の糞尿の悪臭発生抑制乃至脱臭法。3. A Bacillus meg
aterium ) A method for suppressing the generation of malodor in mammalian excrement or using a formulation containing a bacterium belonging to the genus Aserium as an active ingredient.
aterium ）種に属する細菌が、バチルス・メガテリウム
Ｂ１（Bacillus megaterium B1）であることを特徴と
する請求項３に記載の悪臭発生抑制乃至脱臭法。4. The Bacillus megium
A bacterium belonging to the species Aterium ) is Bacillus megaterium B1 ( Bacillus megaterium B1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03317831A JP3122797B2 (en) | 1991-12-02 | 1991-12-02 | Manure odor generation suppressing or deodorizing agent and manure odor generation suppressing or deodorizing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03317831A JP3122797B2 (en) | 1991-12-02 | 1991-12-02 | Manure odor generation suppressing or deodorizing agent and manure odor generation suppressing or deodorizing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05153971A true JPH05153971A (en) | 1993-06-22 |
| JP3122797B2 JP3122797B2 (en) | 2001-01-09 |
Family
ID=18092538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP03317831A Expired - Fee Related JP3122797B2 (en) | 1991-12-02 | 1991-12-02 | Manure odor generation suppressing or deodorizing agent and manure odor generation suppressing or deodorizing method |
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| Country | Link |
|---|---|
| JP (1) | JP3122797B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003056096A1 (en) * | 2001-12-21 | 2003-07-10 | Life Sciences Tgo, Srl | Odor control agent |
| US6974691B2 (en) | 1998-07-13 | 2005-12-13 | Life Science Tgo, S.R.L. | Surface treatment with dormant bacteria and adhering agent to control odor |
| CN103011543A (en) * | 2012-12-27 | 2013-04-03 | 吕津东 | Microorganism deodorant and preparation method thereof |
| EP2856896A1 (en) | 2013-09-23 | 2015-04-08 | Life Science TGO, SRL | Impregnated odour control products and methods of making the same |
| KR102368580B1 (en) * | 2021-10-07 | 2022-02-25 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
-
1991
- 1991-12-02 JP JP03317831A patent/JP3122797B2/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6974691B2 (en) | 1998-07-13 | 2005-12-13 | Life Science Tgo, S.R.L. | Surface treatment with dormant bacteria and adhering agent to control odor |
| US7314748B1 (en) | 1998-07-13 | 2008-01-01 | Life Science Tgo, S.R.L. | Odor control agent |
| WO2003056096A1 (en) * | 2001-12-21 | 2003-07-10 | Life Sciences Tgo, Srl | Odor control agent |
| CN103011543A (en) * | 2012-12-27 | 2013-04-03 | 吕津东 | Microorganism deodorant and preparation method thereof |
| EP2856896A1 (en) | 2013-09-23 | 2015-04-08 | Life Science TGO, SRL | Impregnated odour control products and methods of making the same |
| KR102368580B1 (en) * | 2021-10-07 | 2022-02-25 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
| KR102380358B1 (en) * | 2021-10-07 | 2022-03-30 | 박한용 | Novel strains of high-temperature Bacillus genus having malodor reduction effect on high-temperature livestock manure and livestock manure malodor reduction composition comprising the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3122797B2 (en) | 2001-01-09 |
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