JPH0513200Y2 - - Google Patents
Info
- Publication number
- JPH0513200Y2 JPH0513200Y2 JP4478786U JP4478786U JPH0513200Y2 JP H0513200 Y2 JPH0513200 Y2 JP H0513200Y2 JP 4478786 U JP4478786 U JP 4478786U JP 4478786 U JP4478786 U JP 4478786U JP H0513200 Y2 JPH0513200 Y2 JP H0513200Y2
- Authority
- JP
- Japan
- Prior art keywords
- main body
- hose
- culture
- large diameter
- rubber plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002184 metal Substances 0.000 claims description 20
- 244000005700 microbiome Species 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 239000007788 liquid Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 230000002093 peripheral effect Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
ａ 考案の目的
（産業上の利用分野）
この考案に係るホース接続部が付設される培養
装置は、バイオテクノロジーの分野に於いて、各
種微生物を所定の条件の下で培養する場合に利用
する。[Detailed explanation of the invention] a. Purpose of the invention (industrial application field) The culture device attached with the hose connection part according to this invention is used in the field of biotechnology to grow various microorganisms under specified conditions. Used when culturing.
（従来の技術）
近年バイオテクノロジーの分野で各種微生物を
使用して、遺伝子の組み変え等の実験を行なう事
が多くなつている。(Prior Art) In recent years, in the field of biotechnology, experiments such as genetic modification have been increasingly conducted using various microorganisms.
この様なバイオテクノロジーに関する実験に使
用する微生物は、純粋なものが多量に必要になる
為、実験室に於いて培養する必要がある。 The microorganisms used in such biotechnology experiments require large amounts of pure microorganisms, so they must be cultured in a laboratory.
この為、従来からジヤーフアーメンターと呼ば
れる培養装置を使用して、微生物の培養を行なつ
ていた。 For this reason, microorganisms have traditionally been cultured using a culture device called a jar fermentor.
この培養装置の構造と作用とに就いて、第１図
を用いて簡単に説明すると、培養装置は、各種制
御機構を内装した中空の基台１の上面に、中空円
筒状の支持筒２を介して円筒状の培養器本体３を
設けている。この培養器本体３の上端に固定した
外向フランジ４の上面には、短円筒状のガラスシ
リンダ５の下端部に固定した外向フランジ６を螺
子７，７によつて結合しており、培養器本体３の
上方に、ガラスシリンダ５を液密に装着してい
る。 The structure and operation of this culture device will be briefly explained using FIG. A cylindrical incubator main body 3 is provided therebetween. An outward flange 6 fixed to the lower end of a short cylindrical glass cylinder 5 is connected to the upper surface of an outward flange 4 fixed to the upper end of the incubator main body 3 by screws 7, 7. A glass cylinder 5 is mounted above the cylinder 3 in a liquid-tight manner.
上記培養器本体３の側面には、この培養器本体
３内のPH値や溶存酸素濃度等、微生物の培養に必
要とされる各種データを測定する為のセンサ８が
装着されている。第１図に於いて９は培養器本体
３とガラスシリンダ５とに囲まれた培養空間内の
気体を排出する為の排気管で、途中にフイルタ１
０と開閉弁１１とを設けている。又、ガラスシリ
ンダ５の内側に位置する培養器本体３の上方部分
には、消泡羽根１２を設け、前記支持筒２内を貫
通して設けた回転軸の上端の攪拌羽根によつて培
養器本体３内の培養液を攪拌した場合に発生する
泡を消す様にしている。１３は上記攪拌羽根の回
転時に、培養液の攪拌が効率良く行なわれる様に
する為の邪魔板である。 A sensor 8 is attached to the side surface of the incubator main body 3 to measure various data necessary for culturing microorganisms, such as the PH value and dissolved oxygen concentration within the incubator main body 3. In Fig. 1, 9 is an exhaust pipe for discharging the gas in the culture space surrounded by the culture vessel main body 3 and the glass cylinder 5, and there is a filter 1 in the middle.
0 and an on-off valve 11 are provided. Further, a defoaming blade 12 is provided in the upper part of the incubator main body 3 located inside the glass cylinder 5, and the incubator It is designed to eliminate bubbles generated when the culture solution in the main body 3 is stirred. Reference numeral 13 denotes a baffle plate for efficiently stirring the culture solution when the stirring blade is rotated.
上述の様に構成される培養装置を用いて微生物
の培養を行なう場合、培養器本体３内に培養すべ
き微生物と培養液（培地）とを入れ、この培養器
本体３内の状態をセンサ８からの信号に基いて微
生物の増殖に最適な状態に保ちつつ培養を行な
う。 When culturing microorganisms using the culture device configured as described above, the microorganisms to be cultured and a culture medium (medium) are placed in the incubator main body 3, and the state inside the incubator main body 3 is detected by a sensor 8. Culture is performed while maintaining conditions optimal for microbial growth based on signals from the microorganisms.
培養器本体３は、内筒と外筒とから成る二重構
造とし、内筒の外周面と外筒の内周面との間に存
在する円筒状のジヤケツトの内側に、熱湯送り込
み管１４と排出管１５とを通じて熱湯を送り込み
自在とし、培養液を貯溜した内筒の内側を滅菌処
理出来る様にしている。又、内筒の底部に通じる
サンプリング弁１６の内側にも滅菌用水蒸気を送
り込み自在としている。 The incubator main body 3 has a double structure consisting of an inner cylinder and an outer cylinder, and a hot water feed pipe 14 and a hot water feed pipe 14 are installed inside a cylindrical jacket that exists between the outer peripheral surface of the inner cylinder and the inner peripheral surface of the outer cylinder. Hot water can be fed freely through the discharge pipe 15 to sterilize the inside of the inner cylinder in which the culture solution is stored. Furthermore, sterilizing steam can also be fed into the inside of the sampling valve 16 that communicates with the bottom of the inner cylinder.
（考案が解決しようとする問題点）
ところが、上述の様に構成され使用される培養
装置に於いては、従来は次に述べる様な不都合を
生じた。(Problems to be Solved by the Invention) However, in the culture apparatus constructed and used as described above, conventional inconveniences have occurred as described below.
即ち、遺伝子変換等の高度の実験に使用する純
粋な微生物を得る為には、培養液中に、培養すべ
き微生物のみで、他の微生物が存在しない様にす
る必要がある。 That is, in order to obtain pure microorganisms for use in advanced experiments such as gene conversion, it is necessary to ensure that only the microorganisms to be cultured and no other microorganisms are present in the culture solution.
この為、第１図に示した培養装置に於いては、
前述した通り、培養器本体３とサンプリング弁１
６とを培養作業に先立つて滅菌処理出来る様に構
成しているが、培養作業の進行に伴なつて培養器
本体３内に培養用餌、培養液のPH値調節用の酸、
アルカリ液、或は消泡剤を送り込む為のホース
を、同時に滅菌処理する事は出来ない。 For this reason, in the culture apparatus shown in Figure 1,
As mentioned above, the incubator main body 3 and the sampling valve 1
6 is configured so that it can be sterilized prior to the culture work, but as the culture work progresses, culture food, acid for adjusting the pH value of the culture solution, acid for adjusting the pH value of the culture solution, etc.
It is not possible to sterilize the hose for feeding alkaline solution or antifoaming agent at the same time.
これらホース類は、上記各種液体を貯溜した容
器と共に別途設けた蒸気滅菌器によつて滅菌処理
した後に、培養器本体３の上方開口を塞ぐ蓋体１
７に接続する様にしているが、この接続作業時に
蓋体１７の上面に付着している雑菌が培養器本体
３内に入り込まない様にする必要がある。 These hoses are sterilized together with the containers storing the various liquids mentioned above in a separately provided steam sterilizer, and then the lid body 1 that closes the upper opening of the incubator main body 3 is used.
However, during this connection work, it is necessary to prevent germs adhering to the top surface of the lid 17 from entering the incubator main body 3.
本考案の培養器用ホース接続部は、上述の様な
事情により考案されたものである。 The incubator hose connection part of the present invention was devised in view of the above-mentioned circumstances.
ｂ 考案の構成
（問題を解決するための手段）
本考案の培養装置用ホース接続部は、培養液と
培養すべき微生物とを入れる培養器本体の上端開
口を塞ぐ蓋体に、下半に位置する小径部と上半に
位置する大径部とを段部により連続させた上下方
向に亘る通孔を形成している。b. Structure of the invention (means for solving the problem) The hose connection part for the culture device of the invention is located in the lower half of the lid that closes the upper opening of the incubator main body in which the culture solution and the microorganisms to be cultured are placed. A vertically extending through hole is formed by connecting a small diameter part located in the upper half and a large diameter part located in the upper half by a stepped part.
この通孔の大径部の内周面には雌螺子を刻設
し、上記段部の上面と大径部内周面の雌螺子に螺
着した円管状の抑え金具の下端縁部との間にゴム
板を挟持している。 A female screw is carved into the inner circumferential surface of the large diameter portion of this through hole, and a gap is formed between the upper surface of the stepped portion and the lower end edge of the circular tubular retainer screwed onto the female screw on the inner circumferential surface of the large diameter portion. A rubber plate is held in between.
このゴム板は、円板状の基板の上面中央部に短
円筒状の立壁を形成したもので、この立壁の内側
にはアルコールを注入自在としている。 This rubber plate has a short cylindrical vertical wall formed in the center of the upper surface of a disk-shaped substrate, and alcohol can be freely injected into the inside of this vertical wall.
上記抑え金具の内側にはソケツトを螺入自在と
し、更にこのソケツトの中心に設けた上下方向に
亘る通路の内側には、下端部を鋭利に斜切し、上
端部にホースの端部を接続自在とした金属管を嵌
合している。 A socket can be screwed into the inside of the above-mentioned holding fitting, and inside the vertical passage provided in the center of this socket, the lower end is cut sharply and the end of the hose is connected to the upper end. A flexible metal tube is fitted.
（作用）
上述の様に構成される本考案の培養装置用ホー
ス接続部に於いては、培養作業を開始するのに先
立つて各種液体を培養器本体３内に供給する為の
ホースの端部を蓋体に接続する場合、次に述べる
様にして行なう。(Function) In the hose connection section for the culture device of the present invention configured as described above, the end of the hose is used to supply various liquids into the culture device main body 3 prior to starting the culture work. When connecting the cover to the lid, proceed as follows.
蓋体に形成した通孔には、ゴム板と抑え金具と
を、抑え金具の下端縁と通孔内周面の段部上面と
の間でゴム板の外周縁部を挟持した状態で装着
し、中心に金属管を嵌合したソケツトは、上記金
属管の上端部に一端を接続したホースごと蒸気滅
菌器によつて滅菌処理しておく。 A rubber plate and a retainer are attached to the through hole formed in the lid body, with the outer periphery of the rubber plate being held between the lower edge of the retainer and the upper surface of the step on the inner circumferential surface of the hole. The socket, into which the metal tube is fitted in the center, is sterilized in a steam sterilizer together with a hose connected at one end to the upper end of the metal tube.
培養器本体の内側と、ホース及びこのホースの
両端部に接続した容器、ソケツト、金属管との滅
菌処理が済んだならば、前記ゴム板の上面に形成
した立壁の内側にアルコール（エタノール）を注
入し、このアルコールに着火した状態で、鋭利に
斜切した金属管の下端部を立壁の内側に挿入す
る。 After sterilizing the inside of the incubator body, the hose and the containers, sockets, and metal tubes connected to both ends of the hose, apply alcohol (ethanol) to the inside of the vertical wall formed on the top surface of the rubber plate. With the alcohol injected and ignited, the lower end of the sharply cut metal tube is inserted inside the vertical wall.
立壁の内側に挿入した金属管の下端部は、その
まま立壁に囲まれた基板の中央部に突き刺し、こ
の金属管の下端部を培養器本体の内側に露出させ
る。 The lower end of the metal tube inserted inside the vertical wall is directly stuck into the center of the substrate surrounded by the vertical wall, and the lower end of the metal tube is exposed inside the culture vessel main body.
この作業によつてホースの内側が培養器本体内
に通じ、このホースを通じて送られる各種液体を
培養器本体内に供給自在となるが、この接続作業
の際、接続部はアルコールが燃焼する事で発生す
る炎に覆われている為、培養器本体内に雑菌が入
り込む事はない。 Through this process, the inside of the hose is connected to the incubator main body, and various liquids sent through this hose can be freely supplied into the incubator main body. Since the incubator is covered with flame, no bacteria can enter the incubator itself.
培養作業自体は、従来の培養装置によつて行な
う作業と全く同様である為、説明は省略する。 The culturing operation itself is exactly the same as that performed using a conventional culturing apparatus, so a description thereof will be omitted.
（実施例）
次に、図示の実施例を説明しつつ本考案を更に
詳しく説明する。(Example) Next, the present invention will be explained in more detail by explaining the illustrated embodiment.
第２図は本考案の実施例を示す第１図のＡ部拡
大断面図である。 FIG. 2 is an enlarged sectional view of section A in FIG. 1 showing an embodiment of the present invention.
培養液と培養すべき微生物とを入れる培養器本
体３（第１図）の上端開口を塞ぐ蓋体１７には、
上下方向に亘る通孔１８を形成している。この通
孔１８は、下半に位置する小径部１８ａと上半に
位置する大径部１８ｂとを段部１８ｃにより連続
させたもので、この通孔１８を介して培養器本体
３の内外を連通させている。 The lid body 17 that closes the upper opening of the incubator main body 3 (Fig. 1), which contains the culture solution and the microorganisms to be cultured, includes:
A through hole 18 extending in the vertical direction is formed. This through hole 18 has a small diameter part 18a located in the lower half and a large diameter part 18b located in the upper half connected by a stepped part 18c, and allows the inside and outside of the incubator main body 3 to be connected through this through hole 18. It communicates.
この通孔１８の大径部１８ｂの内周面には雌螺
子１９を刻設し、通孔１８の内側に後述する抑え
金具２０を螺着自在としている。 A female screw 19 is formed on the inner circumferential surface of the large diameter portion 18b of the through hole 18, so that a retainer 20, which will be described later, can be screwed into the inside of the through hole 18.
上記大径部１８ｂの上側に位置する段部１８ｃ
の上面と、大径部１８ｂの内周面の雌螺子１９に
螺着した円管状の抑え金具２０の下端縁部との間
にはゴム板２１を挟持している。 Stepped portion 18c located above the large diameter portion 18b
A rubber plate 21 is sandwiched between the upper surface and the lower end edge of a cylindrical retainer 20 screwed onto a female screw 19 on the inner circumferential surface of the large diameter portion 18b.
このゴム板２１は、円板状の基板２２の上面中
央部に短円筒状の立壁２３を形成し、この立壁２
３の内周面と基板２２の中央部上面とによつて囲
まれる凹部２４を設けたもので、この立壁２３の
内側に位置する凹部２４内にはアルコールを注入
自在としている。 This rubber plate 21 has a short cylindrical vertical wall 23 formed at the center of the upper surface of a disk-shaped substrate 22.
A recess 24 is provided which is surrounded by the inner circumferential surface of No. 3 and the upper surface of the central portion of the substrate 22, and alcohol can be freely poured into the recess 24 located inside the vertical wall 23.
上記抑え金具２０の上部内周面には雌螺子２５
を刻設しており、この雌螺子２５に、抑え金具２
０の内側に挿入したソケツト２６の上部に回転及
び若干の昇降を自在として外嵌した螺子部材３０
の外周面に刻設した雄螺子３１を螺入自在として
いる。即ち、ソケツト２６の中間部外周面に形成
した外向フランジ３２の上側に於いてソケツト２
６に外嵌した螺子部材３０の上面よりも少し上方
に寄つたソケツト２６の外周面にストツプリング
３３を嵌着して、ソケツト２６の上部に螺子部材
３０を、回転及び若干の昇降を自在として装着し
ている。 A female screw 25 is provided on the inner peripheral surface of the upper part of the holding fitting 20.
is engraved on this female screw 25.
A screw member 30 is fitted onto the upper part of the socket 26 inserted into the inside of the screw member 30 so as to be able to rotate and move up and down slightly.
A male screw 31 engraved on the outer circumferential surface of the holder can be inserted freely. That is, the socket 2
A stop ring 33 is fitted to the outer circumferential surface of the socket 26 slightly above the upper surface of the screw member 30 fitted onto the socket 6, and the screw member 30 is attached to the upper part of the socket 26 so that it can be freely rotated and moved up and down slightly. are doing.
更に、このソケツト２６の中心に設けた上下方
向に亘る通路２７の内側には、下端部を鋭利に斜
切し、上端部に各種液体供給用の容器に通じるホ
ース２８の端部を接続自在とした金属管２９を嵌
合している。 Furthermore, a metal tube 29 is fitted into the inside of a vertical passage 27 provided in the center of this socket 26, the lower end of which is sharply beveled and the upper end of which can be freely connected to the end of a hose 28 which leads to a container for supplying various liquids.
上述の様に構成される本考案の培養装置用ホー
ス接続部に於いては、培養作業を開始するのに先
立つて、各種液体を培養器本体３内に供給する為
のホースの端部を蓋体に接続する場合、次に述べ
る様にして行なう。 In the hose connection section for the culture device of the present invention constructed as described above, the ends of the hoses for supplying various liquids into the culture device main body 3 are capped before starting the culture work. When connecting to the body, do so as described below.
蓋体１７に形成した通孔１８には、ゴム板２１
と抑え金具２０とを、抑え金具２０の下端縁と通
孔１８の中間部内周面の段部１８ｃの上面との間
でゴム板２１の外周縁部を挟持した状態で装着し
ておく。この状態に於いては、蓋体１７の上下
（培養器本体３の内外）はゴム板２１によつて遮
断され、培養器本体３外に存在する雑菌が通孔１
８を通じて培養器本体３内に進入する事が防止さ
れる。 A rubber plate 21 is inserted into the through hole 18 formed in the lid body 17.
and a retaining metal fitting 20 are attached with the outer peripheral edge of the rubber plate 21 being sandwiched between the lower edge of the restraining metal fitting 20 and the upper surface of the stepped portion 18c of the inner circumferential surface of the intermediate portion of the through hole 18. In this state, the top and bottom of the lid 17 (inside and outside of the incubator main body 3) are blocked by the rubber plate 21, and bacteria existing outside the incubator main body 3 enter the through hole 1.
8 into the incubator main body 3.
各種液体供給用の容器に通じるホース２８の端
部を上端に接続した金属管２９を中心に嵌合し、
上部外周に螺子部材３０を回転自在に外嵌したソ
ケツト２６は、上記ホース２８ごと別途設けた蒸
気滅菌器（図示せず）によつて滅菌処理してお
く。 It is fitted around a metal pipe 29 whose upper end is connected to the end of a hose 28 leading to containers for supplying various liquids,
The socket 26, in which the screw member 30 is rotatably fitted on the outer periphery of the upper part, is sterilized together with the hose 28 using a steam sterilizer (not shown) provided separately.
熱湯送り込み管１４、排出管１５を通じての熱
湯の循環によつて、培養液が接触する培養器本体
３の内側を滅菌処理し、別途設けた蒸気滅菌器に
よつてホース２８及びこのホース２８の両端部に
接続した容器、ソケツト２６、金属管２９、螺子
部材３０の滅菌処理が済んだならば、前記ゴム板
２１の上面に形成した立壁２３の内側にアルコー
ル（エタノール）を注入し、このアルコールに着
火する。アルコールの燃焼に伴なう炎によつて、
上記立壁２３の上方には雑菌が近付かない状態と
なる為、アルコールが燃焼したままの状態で、鋭
利に斜切した金属管２９の下端部を立壁２３の内
側に挿入する。 By circulating hot water through the hot water feed pipe 14 and the discharge pipe 15, the inside of the culture vessel main body 3 that comes into contact with the culture solution is sterilized, and the hose 28 and both ends of this hose 28 are sterilized using a separately provided steam sterilizer. After the container connected to the section, the socket 26, the metal tube 29, and the screw member 30 have been sterilized, alcohol (ethanol) is injected into the inside of the vertical wall 23 formed on the upper surface of the rubber plate 21, and the alcohol is ignite. Due to the flame that accompanies the combustion of alcohol,
Since germs cannot approach the upper part of the vertical wall 23, the lower end of the sharply cut metal tube 29 is inserted inside the vertical wall 23 while the alcohol is still burning.
無菌状態を保持したまま立壁２３の内側に挿入
した金属管２９の下端部は、そのまま立壁２３に
囲まれた基板２２の中央部に突き刺し、この金属
管２９の下端部を培養器本体３の内側に露出させ
る。 The lower end of the metal tube 29 inserted into the inside of the standing wall 23 while maintaining a sterile state is stuck directly into the center of the substrate 22 surrounded by the standing wall 23, and the lower end of the metal tube 29 is inserted inside the incubator main body 3. to be exposed to.
この作業によつて各種液体を貯溜した容器に通
じるホース２８の内側が培養器本体３内に通じ、
このホース２３を通じて送られる各種液体を培養
器本体３内に供給自在となるので、螺子部材３０
の下部外周面に形成した雄螺子３１を、抑え金具
２０の上部内周面に形成した雌螺子２５に螺合し
緊締して、金属管２９がゴム板２１の中央部から
抜け出るのを防止する。この状態で、抑え金具２
０の内周面とソケツト２６の外周面との間の気密
は、Ｏリング３４によつて保持される。 Through this operation, the inside of the hose 28 leading to the containers storing various liquids is connected to the inside of the incubator main body 3.
Since various liquids sent through this hose 23 can be freely supplied into the culture vessel main body 3, the screw member 30
A male screw 31 formed on the outer peripheral surface of the lower part is screwed into a female screw 25 formed on the inner peripheral surface of the upper part of the holding fitting 20 and tightened to prevent the metal tube 29 from slipping out from the central part of the rubber plate 21. . In this state, hold down metal fitting 2.
Airtightness between the inner circumferential surface of the socket 26 and the outer circumferential surface of the socket 26 is maintained by an O-ring 34.
ｃ 考案の効果
本考案の培養装置用ホース接続部は以上に述べ
た通り構成され作用する為、培養作業に先立つ培
養器本体への各種液体供給用のホース接続を無菌
状態を保持したまま容易に行なうことが出来、微
生物の培養作業を効率良く安定して行なう事が出
来る。c. Effects of the invention Since the hose connection part for the culture device of the present invention is configured and operates as described above, it is possible to easily connect hoses for supplying various liquids to the main body of the culture device prior to culture work while maintaining a sterile state. This allows for efficient and stable microbial culture work.
第１図は培養装置の全体構成を示す正面図、第
２図はホース接続部を示す第１図のＡ部拡大断面
図である。
１……基台、２……支持筒、３……培養器本
体、４……外向フランジ、５……ガラスシリン
ダ、６……外向フランジ、７……螺子、８……セ
ンサ、９……排気管、１０……フイルタ、１１…
…開閉弁、１２……消泡羽根、１３……邪魔板、
１４……熱湯送り込み管、１５……排出管、１６
……サンプリング弁、１７……蓋体、１８……通
孔、１８ａ……小径部、１８ｂ……大径部、１８
ｃ……段部、１９……雌螺子、２０……抑え金
具、２１……ゴム板、２２……基板、２３……立
壁、２４……凹部、２５……雌螺子、２６……ソ
ケツト、２７……通路、２８……ホース、２９…
…金属管、３０……螺子部材、３１……雄螺子、
３２……外向フランジ、３３……ストツプリン
グ、３４……Ｏリング。
Fig. 1 is a front view showing the overall configuration of the culture device, and Fig. 2 is an enlarged cross-sectional view of part A in Fig. 1 showing the hose connection part. 1... base, 2... support tube, 3... culture vessel main body, 4... outward flange, 5... glass cylinder, 6... outward flange, 7... screw, 8... sensor, 9... exhaust pipe, 10... filter, 11...
...on-off valve, 12... defoaming blade, 13... baffle plate,
14: Hot water supply pipe, 15: Discharge pipe, 16
...sampling valve, 17...lid, 18...through hole, 18a...small diameter portion, 18b...large diameter portion, 18
c: step portion, 19: female screw, 20: clamping metal fitting, 21: rubber plate, 22: base plate, 23: vertical wall, 24: recess, 25: female screw, 26: socket, 27: passage, 28: hose, 29:
...metal tube, 30...screw member, 31...male screw,
32: outward flange, 33: stop ring, 34: O-ring.
Claims (1)
体の上端開口を塞ぐ蓋体に、下半に位置する小径
部と上半に位置する大径部とを段部により連続さ
せた上下方向に亘る通孔を形成し、この通孔の大
径部の内周面に雌螺子を刻設し、上記段部の上面
と大径部に螺着した円管状の抑え金具の下端縁部
との間に、円板状の基板の上面中央部に短円筒状
の立壁を形成したゴム板を挟持し、上記抑え金具
の内側に挿入自在なソケツトの中心に形成した上
下方向に亘る通路の内側に、下端部を鋭利に斜切
し、上端部にホースの端部を接続自在とした金属
管を嵌合して成る培養装置用ホース接続部。 A lid body that closes the upper end opening of the incubator main body in which the culture solution and the microorganisms to be cultured are placed has a small diameter part located in the lower half and a large diameter part located in the upper half connected in the vertical direction by a stepped part. A through hole is formed, and a female screw is carved into the inner circumferential surface of the large diameter portion of the through hole, and between the upper surface of the stepped portion and the lower end edge of the circular tubular holding fitting screwed onto the large diameter portion. A rubber plate with a short cylindrical vertical wall formed in the center of the upper surface of the disk-shaped substrate is sandwiched between the two, and the rubber plate is placed inside a vertical passage formed at the center of a socket that can be freely inserted into the inside of the holding fitting. A hose connection part for a culture device, which has a sharply cut lower end and a metal tube fitted to the upper end to which the end of the hose can be freely connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4478786U JPH0513200Y2 (en) | 1986-03-28 | 1986-03-28 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4478786U JPH0513200Y2 (en) | 1986-03-28 | 1986-03-28 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62157400U JPS62157400U (en) | 1987-10-06 |
| JPH0513200Y2 true JPH0513200Y2 (en) | 1993-04-07 |
Family
ID=30862923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4478786U Expired - Lifetime JPH0513200Y2 (en) | 1986-03-28 | 1986-03-28 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0513200Y2 (en) |
-
1986
- 1986-03-28 JP JP4478786U patent/JPH0513200Y2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62157400U (en) | 1987-10-06 |
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