JPH04363655A - Capillary electro-cataphoresis device - Google Patents
Capillary electro-cataphoresis deviceInfo
- Publication number
- JPH04363655A JPH04363655A JP3137529A JP13752991A JPH04363655A JP H04363655 A JPH04363655 A JP H04363655A JP 3137529 A JP3137529 A JP 3137529A JP 13752991 A JP13752991 A JP 13752991A JP H04363655 A JPH04363655 A JP H04363655A
- Authority
- JP
- Japan
- Prior art keywords
- capillary
- detection section
- cell
- detection
- solution tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 claims abstract description 59
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- 238000005251 capillar electrophoresis Methods 0.000 claims description 13
- 238000004458 analytical method Methods 0.000 abstract description 15
- 230000035945 sensitivity Effects 0.000 abstract description 11
- 238000001155 isoelectric focusing Methods 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000011247 coating layer Substances 0.000 description 3
- 239000005350 fused silica glass Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000005515 capillary zone electrophoresis Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明はキャピラリー電気泳動装
置に関し、特にキャピラリーによる検出部のセルと溶液
槽を無駄なく接続することで、等電点電気泳動の分析時
に分離成分の一部を損失することなく検出し、また、キ
ャピラリーによる検出部のセルの形状を変えることで、
分離成分を高感度に検出できるキャピラリー電気泳動装
置を提供することに関する。[Industrial Application Field] The present invention relates to a capillary electrophoresis device, and in particular, by connecting a cell of a detection section using a capillary and a solution tank without waste, a part of separated components is lost during isoelectric focusing analysis. By changing the shape of the cell in the capillary detection section,
The present invention relates to providing a capillary electrophoresis device that can detect separated components with high sensitivity.
【０００２】0002
【従来の技術】キャピラリー電気泳動装置は高分離能で
高速分析する方法として知られており、その装置の概要
は雑誌「表面」，２８（１９９０年）第４０５頁から第
４１５頁に記載されている。この装置はキャピラリーの
両端に電圧を印加し、試料が泳動されて分離した成分を
キャピラリーの一端から１５から２０ｃｍ程度の位置で
検出する様になっていた。また、該検出部はキャピラリ
ーの被覆層を除いて測定用セルとし、キャピラリーに対
して垂直方向に光を透過させていた。この方式は一般的
に採用されており、他の多くの文献などにも同様の方式
が記載されており、その電気泳動装置の構成を図７に示
す。キャピラリー１の両端に溶液槽２，３と電極４，５
を設置し、該電極４，５間には高電圧電源（図示せず）
が接続され、前記キャピラリー１の途中に検出部６を設
けた構成と成っていた。[Prior Art] A capillary electrophoresis device is known as a method for high-speed analysis with high resolution, and an overview of the device is described in the magazine "Surface", 28 (1990), pages 405 to 415. There is. This device applied a voltage to both ends of the capillary, caused the sample to migrate, and detected the separated components at a position approximately 15 to 20 cm from one end of the capillary. Further, the detection section was used as a measurement cell by removing the capillary coating layer, and the light was transmitted in a direction perpendicular to the capillary. This method is generally adopted, and similar methods are described in many other documents, and the configuration of the electrophoresis apparatus is shown in FIG. Solution baths 2 and 3 and electrodes 4 and 5 are placed at both ends of the capillary 1.
A high voltage power source (not shown) is installed between the electrodes 4 and 5.
was connected, and a detection section 6 was provided in the middle of the capillary 1.
【０００３】一方、上記のキャピラリー電気泳動装置を
用いた多くの分析方法が開発されており、その一つに等
電点電気泳動による分析方法があり、ジャーナル・オブ
・クロマトグラフィー，３８７（１９８７年）第１２７
頁から第１３８頁（Ｊｏｕｒｎａｌｏｆ Ｃｈｒｏｍａ
ｔｏｇｒａｐｈｙ，３８７（１９８７）ｐｐ１２７−１
３８）に記載されている。On the other hand, many analytical methods using the above capillary electrophoresis device have been developed, one of which is an analytical method using isoelectric focusing, as reported in Journal of Chromatography, 387 (1987). ) No. 127
Pages 138 to 138 (Journalof Chroma
tography, 387 (1987) pp127-1
38).
【０００４】0004
【発明が解決しようとする課題】上記の従来技術では、
検出部がキャピラリーの一端から１５から２０ｃｍの位
置にある。この従来技術を用いて、ｐＨ勾配による等電
点電気泳動分析を行なった場合には、キャピラリーの全
長に対して電圧を印加するとｐＨ勾配が生じるので、試
料中の各成分が図７の様に目的ｐＨ範囲の等電点（成分
：ａ，ｂ，ｃ，ｄ，ｅ）に分離され、キャピラリーの全
域に点在して分離が完了する。その後、片側の液を取り
換え、分離した成分をセル側（図７の左から右）に泳動
させると、分離された成分の一部（成分：ｄ，ｅ）がキ
ャピラリーの途中に設けた検出部を通り過ぎている故に
、検出できない（図６参照）と言う問題があった。一方
、該検出部はキャピラリーに対して垂直方向に光を透過
させているために、該キャピラリーの内径が光路長とな
っており、微量試料に対する検出下限や高精度の測定に
対する検出感度については考慮されていない。さらに、
キャピラリー電気泳動ではキャピラリーの両端に高電圧
を印加して発生するジュール熱などによるキャピラリー
内での分離能低下を防ぐために、内径１００μｍ以下の
キャピラリーを用いるのが一般的である。従って、短い
光路長は感度の点で大変不利となる問題があった。[Problem to be solved by the invention] In the above conventional technology,
The detection part is located 15 to 20 cm from one end of the capillary. When performing isoelectric focusing analysis using a pH gradient using this conventional technique, a pH gradient occurs when a voltage is applied to the entire length of the capillary, so each component in the sample is It is separated into isoelectric points (components: a, b, c, d, e) in the target pH range, and is scattered throughout the capillary to complete the separation. After that, when the liquid on one side is replaced and the separated components are migrated toward the cell side (from left to right in Fig. 7), some of the separated components (components: d, e) are transferred to the detection section installed in the middle of the capillary. There was a problem in that it could not be detected because it was passing through (see Fig. 6). On the other hand, since the detection section transmits light perpendicularly to the capillary, the inner diameter of the capillary is the optical path length, so consideration must be given to the lower detection limit for trace samples and the detection sensitivity for high-precision measurements. It has not been. moreover,
In capillary electrophoresis, a capillary with an inner diameter of 100 μm or less is generally used in order to prevent a decrease in resolution within the capillary due to Joule heat generated by applying a high voltage to both ends of the capillary. Therefore, there is a problem in that a short optical path length is very disadvantageous in terms of sensitivity.
【０００５】本発明の目的は、キャピラリーの全長を有
効に活用し、等電点電気泳動分析を行なっても分離した
成分の全てを測定できる様にし、更に、他の分析モード
も含めて検出感度を向上させたキャピラリー電気泳動装
置を提供することにある。The purpose of the present invention is to make effective use of the entire length of the capillary, to enable measurement of all separated components even when performing isoelectric focusing analysis, and to improve detection sensitivity including other analysis modes. An object of the present invention is to provide a capillary electrophoresis device with improved performance.
【０００６】[0006]
【課題を解決するための手段】上記目的は、キャピラリ
ーの端部を検出部のセルとして溶液槽に直接接続し、試
料成分を泳動分離するためのキャピラリーの全長を全て
分離帯域とすること、また、検出部のセルの構造をクラ
ンク形状、又は、Ｌ字形状に成形して、その流路方向に
光を透過させ、検出部の光路長を長くすることにより達
成できる。[Means for Solving the Problems] The above object is to connect the end of the capillary directly to a solution bath as a cell of the detection section, and to make the entire length of the capillary a separation zone for electrophoretically separating sample components; This can be achieved by forming the cell structure of the detection section into a crank shape or an L-shape, allowing light to pass in the direction of the flow path, and increasing the optical path length of the detection section.
【０００７】[0007]
【作用】キャピラリー電気泳動装置により、等電点電気
泳動分析を行なった場合には、試料成分中の目的ｐＨ範
囲の各成分がキャピラリーの全域に分離されて点在する
。本発明では、キャピラリーの端部に設けた検出部のセ
ルと溶液槽を最小限の長さで接続し、キャピラリーの全
長を全て分離帯域とすることができる。故に、キャピラ
リーの両端に電圧を印加して試料を泳動すると、試料中
の目的ｐＨ範囲で分離した各成分をキャピラリーの分離
帯域中に留めることができる様になるので、分離した各
成分が検出部のセルを通過することはない。その後、検
出部側に分離した各成分を泳動させることで、分離した
各成分が全て検出できる様に作用するのである。また、
検出部のセルの構造をクランク形状、又は、Ｌ字形状と
し、その流路方向に光を透過させる様にしたことで、光
路長を任意に長く設定することができるので、検出感度
が高められる様になるのである。[Operation] When isoelectric focusing analysis is performed using a capillary electrophoresis device, each component in the target pH range in the sample components is separated and scattered throughout the capillary. In the present invention, the cell of the detection section provided at the end of the capillary and the solution tank can be connected with a minimum length, so that the entire length of the capillary can be used as a separation zone. Therefore, when a voltage is applied to both ends of the capillary and the sample is migrated, each component separated in the target pH range in the sample can be retained in the separation zone of the capillary, so that each separated component is transferred to the detection section. It never passes through the cell. Thereafter, by electrophoresing each separated component toward the detection section, it is possible to detect all of the separated components. Also,
By making the cell structure of the detection part crank-shaped or L-shaped and allowing light to pass in the direction of the flow path, the optical path length can be set arbitrarily long, increasing detection sensitivity. It becomes like that.
【０００８】[0008]
【実施例】図１に示す本発明の一実施例であるキャピラ
リー電気泳動装置は、キャピラリー１の両端部に各々の
電極４，５を設けた溶液槽２，３を設置し、キャピラリ
ー１の片側の端部付近を直角のクランク形状に成形して
検出器のセルとする検出部６を設け、該検出部６と溶液
槽３を接続し、該検出部６の中心線に沿った矢印方向に
検出用の光を透過させ、前記電極４，５間には高電圧電
源８を接続して構成するのである。検出部６はフューズ
ドシリカ管などによるキャピラリー１の端部付近の被覆
層を除いて、直角のクランク形状に成形し、検出用の光
を矢印方向に透過させて検出器のセルとし、また、上記
検出部６の端部と電極５を有する溶液槽３とを接着剤な
どにより接着接続したものである。本発明では、等電点
電気泳動分析を行なった場合を例に動作を説明する。等
電点の異なる試料と両性担体の混合物をキャピラリー１
の中に充填し、溶液槽２，３にはそれぞれ２０ｍＭ水酸
化ナトリウム，２０ｍＭリン酸の溶液を満たす。次に、
該溶液槽２，３に設置した白金の電極４，５間に高電圧
電源８から高電圧を印加すると、キャピラリー１の中の
両性担体はｐＨ勾配を生じて試料中の各成分がキャピラ
リー１内の各等電点の位置（成分：ａ，ｂ，ｃ，ｄ，ｅ
）に分離されて停止する。分離が完了後、溶液槽２を２
０ｍＭ水酸化ナトリウム−８０ｍＭ塩化ナトリウムの溶
液に取り換えて電気泳動をすると、分離した各成分が溶
液槽３側に泳動され、検出部６を通過することでキャピ
ラリー１内で分離された全成分が高感度に検出できるの
である。[Embodiment] A capillary electrophoresis apparatus which is an embodiment of the present invention shown in FIG. A detection part 6 is provided by forming the vicinity of the end into a right-angled crank shape to serve as a detector cell, and the detection part 6 and the solution tank 3 are connected to each other in the direction of the arrow along the center line of the detection part 6. A high voltage power source 8 is connected between the electrodes 4 and 5 to allow detection light to pass therethrough. The detection part 6 is formed into a right-angled crank shape except for a coating layer near the end of the capillary 1 made of a fused silica tube, and transmits detection light in the direction of the arrow to serve as a detector cell. The end of the detection section 6 and the solution tank 3 having the electrode 5 are adhesively connected using an adhesive or the like. In the present invention, the operation will be explained using an example in which isoelectric focusing analysis is performed. A mixture of samples with different isoelectric points and amphoteric carriers is transferred to capillary 1.
and solution tanks 2 and 3 are filled with solutions of 20mM sodium hydroxide and 20mM phosphoric acid, respectively. next,
When a high voltage is applied from the high voltage power supply 8 between the platinum electrodes 4 and 5 installed in the solution baths 2 and 3, the amphoteric carrier in the capillary 1 generates a pH gradient, and each component in the sample flows into the capillary 1. The position of each isoelectric point (components: a, b, c, d, e
) and stop. After the separation is completed, the solution tank 2 is
When the solution is changed to 0mM sodium hydroxide-80mM sodium chloride and electrophoresis is performed, each separated component is migrated to the solution tank 3 side, and as it passes through the detection part 6, all the components separated in the capillary 1 become highly concentrated. It can be detected with high sensitivity.
【０００９】本発明の他の実施例による検出部を図２に
示す。検出部６はフューズドシリカ管などによるキャピ
ラリー１の端部付近の被覆層７を除いて、鈍角度のクラ
ンク形状に成形し、検出用の光を矢印方向に透過させて
検出器のセルとし、また、上記検出部６の端部と電極５
を有する溶液槽３とを接着剤などにより接着接続して構
成したものである。動作は前述図１と同様であるが、図
１に比べて検出部の強度が高く、分離した成分のピーク
の拡がりが少ない利点がある。A detection section according to another embodiment of the present invention is shown in FIG. The detection part 6 is formed into a crank shape with an obtuse angle, excluding the coating layer 7 near the end of the capillary 1 made of a fused silica tube, etc., and transmits detection light in the direction of the arrow to form a detector cell. In addition, the end of the detection section 6 and the electrode 5
It is constructed by adhesively connecting a solution tank 3 with a solution tank 3 using an adhesive or the like. The operation is similar to that shown in FIG. 1 described above, but compared to FIG. 1, there is an advantage that the intensity of the detection section is higher and the spread of the peaks of separated components is smaller.
【００１０】本発明の変形例を図３に示す。検出部６は
キャピラリー１の端部付近をＬ字形状に成形した端部と
、電極５を有する石英製の皿状である溶液槽３の底部を
ほぼ接触させた構成である。検出用の光は前記Ｌ字形状
のセルの内部を矢印方向に透過し、更に、皿状の溶液槽
３の底部を透過させるのである。この変形例は容易に製
作できる利点がある。A modification of the present invention is shown in FIG. The detecting section 6 has a configuration in which an L-shaped end portion of the capillary 1 is substantially in contact with the bottom of a quartz dish-shaped solution tank 3 having an electrode 5. The detection light passes through the inside of the L-shaped cell in the direction of the arrow, and further passes through the bottom of the dish-shaped solution tank 3. This modification has the advantage of being easy to manufacture.
【００１１】本発明の簡易例を図４に示す。検出部６は
キャピラリー１の端部付近を直管のまま検出器のセルと
し、該キャピラリー１の端部と溶液槽３を接続した構成
である。この簡易例は、検出光を矢印の様にキャピラリ
ーに対して垂直に透過させるので、検出感度の点で不利
であるが、極めて簡単に製作できる利点がある。A simple example of the invention is shown in FIG. The detection unit 6 has a structure in which the vicinity of the end of the capillary 1 is kept as a straight pipe as a detector cell, and the end of the capillary 1 is connected to the solution tank 3. In this simple example, the detection light is transmitted perpendicularly to the capillary as shown by the arrow, so it is disadvantageous in terms of detection sensitivity, but it has the advantage of being extremely easy to manufacture.
【００１２】本発明の実施例１から４では、吸光検出方
式を示したが蛍光検出方式も同様に適用できる。また、
等電点電気泳動分析以外のキャピラリーゾーン電気泳動
分析などの他の分析モードにも応用が可能である。[0012] In Examples 1 to 4 of the present invention, an absorption detection method is shown, but a fluorescence detection method is also applicable. Also,
It can also be applied to other analysis modes other than isoelectric focusing analysis, such as capillary zone electrophoresis analysis.
【００１３】本発明の実施例によるエレクトロフェログ
ラムを図５に示した。キャピラリー電気泳動装置は本発
明の実施例図１に基づいた装置を用いた。キャピラリー
には内径５０μｍ，外径３７５μｍ，長さ２０ｃｍのフ
ューズドシリカ管、溶液槽には３ｍｌのガラス容器、電
源には関西電子社製のＡＫＴ−３０Ｋ０５Ｐ型，検出器
には日立製紫外吸光検出器Ｌ−４０００型を改造して用
いた。検出部のセルの光路長は約１ｍｍである。測定条
件としては、等電点の異なるペプチド類の混合試料（成
分不明）と両性担体のファルマライトｐＨ３−１０（フ
ァルマシア社製）を混合してキャピラリー内に充填し、
各溶液槽の検出側には２０ｍＭリン酸、他方には２０ｍ
Ｍ水酸化ナトリウム、又は、２０ｍＭ水酸化ナトリウム
−８０ｍＭ塩化ナトリウムの各溶液を用いた。電圧は１
０ＫＶ、検出は波長２００ｎｍである。本発明では、分
離された各成分（ａ，ｂ，ｃ，ｄ，ｅ）が全て高感度に
検出されている。An electropherogram according to an embodiment of the present invention is shown in FIG. As a capillary electrophoresis device, a device based on the embodiment of the present invention shown in FIG. 1 was used. The capillary is a fused silica tube with an inner diameter of 50 μm, an outer diameter of 375 μm, and a length of 20 cm. The solution tank is a 3 ml glass container. The power source is Kansai Denshi's AKT-30K05P model. The detector is Hitachi's ultraviolet absorption detector. A modified model L-4000 was used. The optical path length of the cell of the detection section is approximately 1 mm. The measurement conditions were as follows: A mixed sample of peptides with different isoelectric points (components unknown) and an amphoteric carrier Pharmalite pH 3-10 (manufactured by Pharmacia) were mixed and filled into a capillary.
20mM phosphoric acid on the detection side of each solution tank and 20mM on the other side.
A solution of M sodium hydroxide or 20mM sodium hydroxide-80mM sodium chloride was used. The voltage is 1
0 KV, detection is at a wavelength of 200 nm. In the present invention, all of the separated components (a, b, c, d, e) are detected with high sensitivity.
【００１４】これに対して、従来例図７に基づく装置に
よるエレクロフェログラムを図６に示したが、検出感度
は本発明の１／１０位であり、しかも、測定すべき成分
のｄ，ｅが検出されないと言う欠点が見られた。測定条
件等は前記本発明の実施例と同様である。On the other hand, although the electropherogram obtained by the conventional device based on FIG. 7 is shown in FIG. 6, the detection sensitivity is about 1/10 of that of the present invention, and moreover, The drawback was that it was not detected. The measurement conditions and the like are the same as in the embodiments of the present invention.
【００１５】[0015]
【発明の効果】本発明によれば、キャピラリーの検出部
と溶液槽を接続し、キャピラリーの全長を全て分離帯域
とすることで、キャピラリーの全長を有効に活用でき、
等電点電気泳動分析によるキャピラリー内で分離された
成分を全て検出でき、検出部の光路長を長くしたことで
従来の１０倍程度に高感度検出されたキャピラリー電気
泳動装置を提供できる効果がある。[Effects of the Invention] According to the present invention, by connecting the detection part of the capillary to the solution tank and making the entire length of the capillary a separation zone, the entire length of the capillary can be effectively utilized.
All components separated within the capillary can be detected by isoelectric focusing analysis, and by increasing the optical path length of the detection section, it has the effect of providing a capillary electrophoresis device with detection sensitivity about 10 times higher than conventional methods. .
【図１】本発明の一実施例を示す電気泳動装置の構成図
である。FIG. 1 is a configuration diagram of an electrophoresis apparatus showing an embodiment of the present invention.
【図２】本発明の他の実施例を示す検出部の構成図であ
る。FIG. 2 is a configuration diagram of a detection section showing another embodiment of the present invention.
【図３】本発明の変形例を示す検出部の構成図である。FIG. 3 is a configuration diagram of a detection section showing a modification of the present invention.
【図４】本発明の簡易例を示す検出部の構成図である。FIG. 4 is a configuration diagram of a detection section showing a simple example of the present invention.
【図５】本発明によるエレクトロフェログラムを示す図
である。FIG. 5 shows an electropherogram according to the invention.
【図６】従来例によるエレクトロフェログラムを示す図
である。FIG. 6 is a diagram showing an electropherogram according to a conventional example.
【図７】従来例を示す電気泳動装置の構成図である。FIG. 7 is a configuration diagram of an electrophoresis apparatus showing a conventional example.
１…キャピラリー、２，３…溶液槽、４，５…電極、６
…検出部、７…被覆層、８…高電圧電源。1... Capillary, 2, 3... Solution bath, 4, 5... Electrode, 6
...detection section, 7...covering layer, 8...high voltage power supply.
Claims (3)
該キャピラリーの両端に溶液槽を設け、該溶液槽の各々
に設置した電極間に接続された高電圧電源を有する電気
泳動装置において、前記キャピラリーの片側の端部付近
に測光用セルを形成し、そのセルの長さ方向に向って光
を照射するように構成することを特徴とするキャピラリ
ー電気泳動装置。1. An electrophoresis apparatus comprising a capillary for electrophoresing a sample, a solution tank at both ends of the capillary, and a high voltage power source connected between electrodes installed in each of the solution tanks, wherein the capillary A capillary electrophoresis device characterized in that a photometric cell is formed near one end of the capillary electrophoresis device, and light is irradiated in the length direction of the cell.
該キャピラリーの両端に溶液槽を設け、該溶液槽の各々
に設置した電極間に接続された高電圧電源、及び前記キ
ャピラリーの一部に設けられた検出部を有する検出器な
どから成る電気泳動装置において、前記キャピラリーの
片側の端部付近を光による検出器のセルとする検出部を
設け、該キャピラリーによる検出部のセルの形状がクラ
ンク形状、又は、Ｌ字形状であり、その流路方向に光を
透過させて検出部の光路を形成する構造であり、該検出
部のセルと溶液槽を接続して構成することを特徴とする
キャピラリー電気泳動装置。2. A capillary for electrophoresing a sample, a solution tank at both ends of the capillary, a high voltage power supply connected between electrodes installed in each of the solution tanks, and a high voltage power supply installed in a part of the capillary. In an electrophoresis device comprising a detector or the like having a detection section, the detection section is provided with a cell of a light detector near one end of the capillary, and the cell shape of the detection section using the capillary is crank-shaped. , or a capillary that is L-shaped and has a structure that transmits light in the flow path direction to form an optical path of the detection section, and is configured by connecting a cell of the detection section and a solution tank. Electrophoresis device.
該キャピラリーの両端に溶液槽を設け、該溶液槽の各々
に設置した電極間に接続された高電圧電源、及び前記キ
ャピラリーの一部に設けられた検出部を有する検出器な
どから成る電気泳動装置において、前記キャピラリーの
片側の端部付近を光による検出器のセルとする検出部を
設け、該キャピラリーによる検出部のセルの形状が直線
形状であり、その流路の直角方向に光を透過させて検出
部の光路を形成し、該検出部のセルと溶液槽を接続して
構成することを特徴とするキャピラリー電気泳動装置。3. A capillary for electrophoresing a sample, a solution tank at both ends of the capillary, a high voltage power supply connected between electrodes installed in each of the solution tanks, and a high voltage power supply installed in a part of the capillary. In an electrophoresis apparatus consisting of a detector having a detection section, the detection section is provided with a cell of a light detector near one end of the capillary, and the cell shape of the detection section using the capillary is linear. A capillary electrophoresis device characterized in that an optical path of a detection section is formed by transmitting light in a direction perpendicular to the flow path, and a cell of the detection section and a solution tank are connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3137529A JPH04363655A (en) | 1991-06-10 | 1991-06-10 | Capillary electro-cataphoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3137529A JPH04363655A (en) | 1991-06-10 | 1991-06-10 | Capillary electro-cataphoresis device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04363655A true JPH04363655A (en) | 1992-12-16 |
Family
ID=15200812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3137529A Pending JPH04363655A (en) | 1991-06-10 | 1991-06-10 | Capillary electro-cataphoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04363655A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007212449A (en) * | 2006-01-16 | 2007-08-23 | Hitachi High-Technologies Corp | Capillary electrophoresis apparatus and electrophoresis method |
-
1991
- 1991-06-10 JP JP3137529A patent/JPH04363655A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007212449A (en) * | 2006-01-16 | 2007-08-23 | Hitachi High-Technologies Corp | Capillary electrophoresis apparatus and electrophoresis method |
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