JPH04339257A - Standard color testing body and manufacture thereof - Google Patents
Standard color testing body and manufacture thereofInfo
- Publication number
- JPH04339257A JPH04339257A JP5374691A JP5374691A JPH04339257A JP H04339257 A JPH04339257 A JP H04339257A JP 5374691 A JP5374691 A JP 5374691A JP 5374691 A JP5374691 A JP 5374691A JP H04339257 A JPH04339257 A JP H04339257A
- Authority
- JP
- Japan
- Prior art keywords
- light absorption
- color
- test
- standard color
- absorption characteristics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 146
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- 230000031700 light absorption Effects 0.000 claims abstract description 55
- 239000000126 substance Substances 0.000 claims abstract description 15
- 239000000049 pigment Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 abstract description 27
- 230000000007 visual effect Effects 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 238000012790 confirmation Methods 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 41
- 239000008103 glucose Substances 0.000 description 41
- 238000009534 blood test Methods 0.000 description 24
- 239000000463 material Substances 0.000 description 22
- 239000008280 blood Substances 0.000 description 21
- 210000004369 blood Anatomy 0.000 description 21
- 238000004040 coloring Methods 0.000 description 18
- 210000002700 urine Anatomy 0.000 description 11
- 238000011179 visual inspection Methods 0.000 description 11
- 239000000123 paper Substances 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 9
- 239000000975 dye Substances 0.000 description 8
- AOMZHDJXSYHPKS-DROYEMJCSA-L Amido Black 10B Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=CC2=CC(S([O-])(=O)=O)=C(\N=N\C=3C=CC=CC=3)C(O)=C2C(N)=C1\N=N\C1=CC=C(N(=O)=O)C=C1 AOMZHDJXSYHPKS-DROYEMJCSA-L 0.000 description 6
- 230000001575 pathological effect Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000006096 absorbing agent Substances 0.000 description 3
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000001045 blue dye Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 230000002485 urinary effect Effects 0.000 description 2
- OBHRVMZSZIDDEK-UHFFFAOYSA-N urobilinogen Chemical compound CCC1=C(C)C(=O)NC1CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(CC3C(=C(CC)C(=O)N3)C)N2)CCC(O)=O)N1 OBHRVMZSZIDDEK-UHFFFAOYSA-N 0.000 description 2
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- AOMZHDJXSYHPKS-UHFFFAOYSA-L disodium 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=CC2=CC(S([O-])(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 AOMZHDJXSYHPKS-UHFFFAOYSA-L 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- -1 spraying Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は、標準色試験体およびそ
の調製方法に関する。詳細には、尿検査や血液検査等の
病理検査で使用される呈色試験片や呈色試験紙等の呈色
試験具の呈色結果を比較判定する際に使用されている標
準色試験体の改良に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a standard color test specimen and a method for preparing the same. In detail, it is a standard color test material used to compare and judge the color results of color test devices such as color test strips and color test paper used in pathological tests such as urine tests and blood tests. Regarding improvements.
【０００２】0002
【従来の技術】尿中の潜血、ブドウ糖、蛋白等の有無や
濃度を調べることによって、特定の疾病の有無や疾病状
態の軽重を判定することが可能である。そのために、尿
中の潜血、ブドウ糖、蛋白等の有無やその濃度に応じて
、特定の色に発色または色変化する呈色試験片、それを
接合した呈色試験紙等の呈色試験具が、その簡便さや比
較的正確な結果を与える点から各種医療機関や健康診断
等において広く使用されている。そして、呈色試験具を
使用する病理検査では、濃度既知の検体または疑似検体
によって呈色試験具を呈色させた標準色試験体、または
該標準色試験体と同じ色になるように安定な色素を用い
て調製した標準色試験体を予め別に準備しておき、検査
の結果得られた呈色試験具の呈色状態をそれらの標準色
試験体の色と目視により対比することによって、尿中の
潜血、ブドウ糖、蛋白等の有無や濃度の判定が行われて
きた。2. Description of the Related Art By examining the presence and concentration of occult blood, glucose, protein, etc. in urine, it is possible to determine the presence or absence of a specific disease and the severity of the disease state. For this purpose, color test devices such as color test strips that develop or change color depending on the presence or absence of occult blood, glucose, protein, etc. in urine and their concentration, and color test paper bonded with the test strips, are used. It is widely used in various medical institutions and health checkups because of its simplicity and relatively accurate results. In pathological examinations using color test devices, the color test device is colored with a specimen of known concentration or a pseudo specimen, or a standard color test material is used that is stable so as to have the same color as the standard color test material. A standard color test material prepared using a dye is prepared separately in advance, and the color state of the color test device obtained as a result of the test is visually compared with the color of those standard color test materials. The presence and concentration of occult blood, glucose, protein, etc. inside has been determined.
【０００３】ところで、近年になって、病理検査を短時
間に多数、正確に行うことが必要になり、そのために種
々の検査機械が使用されるようになっている。このこと
は呈色試験具を用いる病理検査においても例外ではなく
、呈色試験具の呈色状態を読取るための光学読取り機が
種々開発され使用されるようになっている。そして、呈
色試験具の呈色状態を光学読取り機を使用して検査する
場合にも、読取り機の動作確認および調整のために、濃
度既知の検体または疑似検体によって呈色試験具を呈色
させた標準色試験体、または該標準色試験体と同じ色に
なるように安定な色素を用いて着色した標準色試験体が
用いられている。Incidentally, in recent years, it has become necessary to accurately perform a large number of pathological examinations in a short period of time, and various examination machines have come to be used for this purpose. This is no exception in pathological examinations using color test tools, and various optical readers have been developed and used to read the coloration state of color test tools. When inspecting the coloring state of a coloring test device using an optical reader, the coloring test device is colored with a sample of known concentration or a pseudo sample in order to confirm and adjust the operation of the reader. A standard color test piece colored with a stable dye to have the same color as the standard color test piece is used.
【０００４】しかしながら、濃度既知の検体または疑似
検体によって呈色試験具を呈色させた標準色試験体は、
呈色状態が比較的不安定であって短期間のうちに初めの
呈色状態と異なった色に変化してしまうために検査のた
びごとに標準色試験体をつくる必要があり繁雑であった
。また、濃度既知の検体または疑似検体によって呈色さ
せた呈色試験具の色と同じ色になるように安定な色素を
用いて調製された標準色試験体の場合は、特定の光学読
取り機に適するように作成されているために、他の読取
り機に対しては使用できない場合が多く互換性がなく、
しかも目視による判定結果と一致しない等の問題があっ
た。[0004] However, the standard color test material, which is made by coloring a color test device with a sample or a pseudo sample of known concentration,
Because the coloration state is relatively unstable and changes to a color different from the initial coloration state within a short period of time, it is necessary to prepare a standard color specimen for each inspection, which is complicated. . In addition, in the case of a standard color test specimen prepared using a stable dye so that the color is the same as the color of the color test device developed by a sample or pseudo-specimen of known concentration, it is necessary to use a specific optical reader. Because they are designed to be compatible with other readers, they are often not compatible with other readers.
Moreover, there was a problem that the results did not match the visually determined results.
【０００５】[0005]
【発明の内容】上記の点から、本発明者は、種々の光学
読取り機に対して互換性があってそれらの動作確認およ
び調整のために有効に且つ共通して使用できると共に、
目視による標準色試験体としても使用でき、光学読取り
機による場合と目視による場合とで正確で且つ同じ判定
結果を得ることができる標準色試験体を開発することを
目的として研究を行ってきた。その結果、安定な色素や
その他の特定の波長領域に光吸収特性を有する物質を用
いて、標準色試験体の光吸収特性を、個々の病理検査に
用いられているのと同じ呈色試験具を使用して濃度既知
の実試料（実際の検体）または疑似検体により呈色させ
た場合の光吸収特性と同じかまたは近似したものになる
ようにすると、上記の目的を達成できることを見出して
本発明を完成した。SUMMARY OF THE INVENTION In light of the above, the present inventor has devised a system that is compatible with various optical readers and can be effectively and commonly used for checking and adjusting their operations.
We have conducted research with the aim of developing a standard color test sample that can be used as a standard color test sample for visual inspection, and that can provide accurate and identical judgment results both when using an optical reader and when visually inspecting. As a result, by using stable dyes and other substances that have light absorption properties in specific wavelength ranges, the light absorption properties of standard color specimens can be measured using the same color test equipment used for individual pathological examinations. This book was based on the discovery that the above purpose could be achieved if the light absorption characteristics were the same or similar to those obtained when a real sample (actual sample) or a pseudo sample of known concentration was used to develop a color. Completed the invention.
【０００６】したがって、本発明は、濃度既知の実検体
または疑似検体により呈色させた呈色試験具の光吸収特
性と同じかまたは近似した光吸収特性を有するように色
素および／またはその他の特定の波長領域に光吸収特性
を有する物質を担持させてなることを特徴とする標準色
試験体である。更に、本発明は、濃度既知の実検体また
は疑似検体により呈色させた呈色試験具の光吸収特性を
測定し、この光吸収特性と同じかまたは近似した光吸収
特性になるように、色素および／またはその他の特定の
波長領域に光吸収特性を有する物質を調合し、この調合
物を使用して上記の濃度既知の実検体または疑似検体に
より呈色させた呈色試験具の光吸収特性と同じまたは近
似した光吸収特性を有する標準色試験体を製造する方法
である。[0006] Therefore, the present invention is directed to the use of dyes and/or other specific materials so as to have light absorption characteristics that are the same as or similar to the light absorption characteristics of a colored test device colored by an actual sample or a pseudo sample of known concentration. This is a standard color test specimen characterized by supporting a substance that has light absorption characteristics in the wavelength range of . Furthermore, the present invention measures the light absorption characteristics of a coloring test device colored with an actual sample or a pseudo sample of known concentration, and measures the light absorption characteristics of the coloring test device so that the light absorption characteristics are the same as or similar to the measured light absorption characteristics. and/or other substances that have light absorption characteristics in a specific wavelength range, and the light absorption characteristics of a color test device that is colored using the above-mentioned actual sample or pseudo sample with a known concentration using this mixture. This is a method for producing a standard color test specimen having the same or similar light absorption properties.
【０００７】ここで、本発明でいう「濃度既知の実検体
または疑似検体により呈色させた呈色試験具の光吸収特
性と同じかまたは近似した光吸収特性を有する」とは、
標準色試験体の光吸収特性が、可視光線領域、可視光線
領域−赤外線領域、可視光線領域−紫外線領域、または
可視光線領域−赤外線領域−紫外線領域において、光学
読取り機で使用される測定波長に応じて、呈色試験具の
それらの領域における光吸収特性と同じかまたは近似し
ていることを意味する。[0007] Here, as used in the present invention, "having a light absorption characteristic that is the same as or similar to the light absorption characteristic of a coloring test device colored by an actual specimen or a pseudo specimen of known concentration" means:
The light absorption properties of the standard color test specimen are determined by the measurement wavelength used by the optical reader in the visible light range, visible light range - infrared range, visible light range - ultraviolet range, or visible light range - infrared range - ultraviolet range. Accordingly, it means that the light absorption properties in those areas of the color test device are the same or similar.
【０００８】上記の点を例を挙げて以下にわかり易く説
明する。例えば、ヘモグロビン偽ペルオキシダーゼ活性
法によって尿中の潜血の有無を検査するのに使用される
呈色試験具である潜血試験片、およびグルコースオキシ
ダーゼ法によって尿中のブドウ糖の有無を検査する呈色
試験具であるブドウ糖試験片では、いずれも呈色剤とし
てオルト−トリジンの酸化物が一般に用いられている。
この潜血試験片またはブドウ糖試験片は、尿中に潜血ま
たはブドウ糖が存在すると、オルト−トリジン酸化物の
働きによって青色に呈色する。そのため、この潜血試験
片またはブドウ糖試験片と組み合わせて使用される標準
色試験片としては、潜血試験片やブドウ糖試験片の呈色
時の青色と目視した場合に同色になるように、青色色素
［例えばアミドブラック１０Ｂ（ＭＥＲＣＫ社製；ＣＩ
２０４７０）等］を用いて着色させた青色標準色試験
紙（例えば、ＰＡＮＴＯＮＥ社製３６０Ｕ等）が用いら
れている。The above points will be explained below in an easy-to-understand manner using an example. For example, an occult blood test piece is a coloring test device used to test for the presence of occult blood in urine using the hemoglobin pseudoperoxidase activity method, and a coloring test device is used to test for the presence of glucose in urine using the glucose oxidase method. In all glucose test strips, ortho-tolidine oxide is generally used as a coloring agent. This occult blood test piece or glucose test piece turns blue due to the action of ortho-tolidine oxide when occult blood or glucose is present in the urine. Therefore, the standard color test piece used in combination with this occult blood test piece or glucose test piece is a blue dye [ For example, Amido Black 10B (manufactured by MERCK; CI
A blue standard color test paper (for example, 360U manufactured by PANTONE) is used.
【０００９】そして、目視による従来の潜血試験または
ブドウ糖試験では、標準色試験片の青色と同じような色
に潜血試験片またはブドウ糖試験片が呈色したか否かを
見ることによって、尿中の潜血の有無を判定することが
できる。一方、測定波長領域が例えば４００ｎｍ〜９０
０ｎｍの黄色領域〜赤外線領域にある光学読取り機を用
いて上記潜血試験片またはブドウ糖試験片の呈色状態を
検査する場合は、上記の青色色素であるアミドブラック
１０Ｂにより着色した標準色試験紙またはＰＡＮＴＯＮ
Ｅ社製３６０Ｕによってまず装置の動作確認および調整
を行った後、潜血試験片またはブドウ糖試験片の呈色状
態を光学的に測定し、標準色試験片の青色と対比して潜
血またはブドウ糖の有無を自動的に判定する。In the conventional visual occult blood test or glucose test, the occult blood test piece or glucose test piece is colored in a color similar to the blue color of the standard color test piece. The presence or absence of occult blood can be determined. On the other hand, the measurement wavelength range is, for example, 400 nm to 90 nm.
When inspecting the coloring state of the occult blood test piece or glucose test piece using an optical reader in the 0 nm yellow range to infrared range, use standard color test paper colored with the above blue dye Amido Black 10B or PANTON
After first checking and adjusting the operation of the device using 360U manufactured by Company E, the coloring state of the occult blood test piece or glucose test piece is optically measured, and the presence or absence of occult blood or glucose is determined by comparing it with the blue color of the standard color test piece. Automatically determine.
【００１０】そして、尿中の潜血またはブドウ糖により
青色に呈色した上記の潜血試験片またはブドウ糖試験片
は、上記光学読取り機を使用して測定した場合に、図１
および図２に示すように、４００ｎｍ〜９００ｎｍの波
長領域、すなわち可視光線領域〜赤外線領域にわたって
吸収領域を有している。それに対して、アミドブラック
１０Ｂにより着色した標準色試験紙は、図３に示すよう
に、４００ｎｍ〜７２０ｎｍに吸収領域を有し赤外線領
域には吸収をもたない。またＰＡＮＴＯＮＥ社製３６０
Ｕは、図４に示すように、その光吸収領域はほぼ４００
ｎｍ〜８００ｎｍであり８００ｎｍ〜９００ｎｍには吸
収をもたず、しかもその吸収曲線の形状は図１および図
２で示される潜血試験片およびブドウ糖試験片の吸収曲
線の形状と大きく相違している。[0010]The above occult blood test piece or glucose test piece, which was colored blue due to occult blood or glucose in the urine, was measured using the optical reader as shown in Fig. 1.
As shown in FIG. 2, it has an absorption region over a wavelength region of 400 nm to 900 nm, that is, a visible light region to an infrared region. On the other hand, the standard color test paper colored with Amido Black 10B has an absorption region from 400 nm to 720 nm and no absorption in the infrared region, as shown in FIG. Also, PANTONE 360
As shown in FIG. 4, U has a light absorption area of approximately 400
nm to 800 nm and has no absorption in the 800 nm to 900 nm range, and the shape of the absorption curve is greatly different from that of the occult blood test piece and the glucose test piece shown in FIGS. 1 and 2.
【００１１】したがって目視による場合には、潜血試験
片およびブドウ糖試験片の青色と、アミドブラック１０
Ｂにより着色された標準色試験片またはＰＡＮＴＯＮＥ
社製３６０Ｕは、いずれも同じ青色を呈し、尿中の潜血
またはブドウ糖の有無の判定が可能であるのに対して、
光学読取り機による場合には、標準色試験片と潜血試験
片またはブドウ糖試験片とで光吸収特性が大きく異なっ
ており、正確な判定ができないという結果を生ずる。そ
れに対して、本発明の標準色試験体においては、標準色
試験体の光吸収特性を、可視光線領域のみでなく、ヒト
の目には見えない赤外線領域および／または紫外線領域
においても実検体または疑似検体を用いて呈色させた呈
色試験具の光吸収特性と同じかまたは近似したものにな
るように調整するので、目視による場合は勿論、光学読
取り機を使用した場合にも正確な判定が可能になる。例
えば、図３で示す光吸収特性を有するアミドブラック１
０Ｂを使用して着色した標準色試験片の場合には、赤外
線領域に吸収を有する物質であるＡｌｄｒｉｃｈ社製Ｉ
Ｒ−１４０をアミドブラック１０Ｂと混合使用すること
によって、標準色試験片の光吸収特性を潜血試験片およ
びブドウ糖試験片の光吸収特性と近似したものにするこ
とができる。Therefore, in the case of visual inspection, the blue color of the occult blood test piece and the glucose test piece and the difference between the blue color of the occult blood test piece and the amide black 10
Standard color test piece colored by B or PANTONE
The company's 360U all exhibit the same blue color and can determine the presence or absence of occult blood or glucose in the urine.
When using an optical reader, the standard color test piece and the occult blood test piece or glucose test piece have significantly different light absorption characteristics, resulting in an inability to make accurate determinations. In contrast, in the standard color test material of the present invention, the light absorption characteristics of the standard color test material can be compared with the actual specimen not only in the visible light region but also in the infrared region and/or ultraviolet region, which are invisible to the human eye. Since the light absorption characteristics are adjusted to be the same as or similar to the light absorption characteristics of the color test device, which is colored using a pseudo specimen, accurate judgment can be made not only by visual inspection but also by using an optical reader. becomes possible. For example, amido black 1 having the light absorption characteristics shown in FIG.
In the case of standard color test pieces colored using 0B, Aldrich's I, which is a substance that absorbs in the infrared region,
By using R-140 in combination with Amido Black 10B, the light absorption characteristics of the standard color test strip can be made similar to those of the occult blood test strip and the glucose test strip.
【００１２】上記では可視光線領域および赤外線領域に
吸収を有する潜血試験片およびブドウ糖試験片並びにそ
のための標準色試験体について説明したが、例えば可視
光線領域のみ、可視光線領域と紫外線領域、可視光線領
域と赤外線領域と紫外線領域に吸収を有する呈色試験具
並びにそのための標準色試験体においても、標準色試験
体の光吸収特性を呈色試験具の光吸収特性と同じかまた
は近似したものにすることによって、目視および光学読
取り機による判定の両方に対して共通して使用でき、種
々の光学読取り機に対して互換性を有し、しかも目視に
よる場合と光学読取り機との両方において正確で且つ同
じ結果が得られるようにすることができる。[0012] In the above, the occult blood test piece and the glucose test piece, which have absorption in the visible light region and the infrared region, and the standard color test material therefor have been explained. In the case of color test devices that absorb in the infrared and ultraviolet regions, and standard color test materials used therefor, the light absorption characteristics of the standard color test material should be the same as or similar to the light absorption characteristics of the color test device. As a result, it can be commonly used for both visual inspection and optical reader determination, is compatible with various optical readers, and is accurate and accurate for both visual inspection and optical reader determination. You can get the same result.
【００１３】上記では尿中の潜血およびブドウ糖を例に
挙げて説明してきたが、本発明でいう呈色試験具は、尿
中の潜血またはブドウ糖の検査に使用される呈色試験具
に限定されない。すなわち、本発明でいう呈色試験具に
は、ヒトや動物の尿、血液、骨髄液、汗、涙、唾液等の
被検査液体を、浸漬、滴下、注入、散布、塗布等の任意
の手段によって呈色試験具に施した場合に、例えば潜血
、ブドウ糖、蛋白質、ウロブリノーゲン、ケトン体、ビ
サルビン、ｐＨ、亜硝酸塩等の被検査液体中に含まれる
特定の物質の存在や濃度に応じて、発色または色変化し
て、それらの物質の有無や濃度を知ることができる試験
具のいずれもが包含される。呈色試験具は、通常、各被
検査物質に対応して反応する特定の試薬や酵素等を濾紙
等に含浸または担持させた試験片あるいは試験片をプラ
スチックテープ等のベース材料に取り付けた形態で用い
らることが多いが、本発明でいう呈色試験具は、その素
材や形態、形状、寸法等はどのようなものであってもよ
く特に限定されない。例えば、呈色試験具は、試験片ま
たは試験紙の他に、任意の形状および寸法の繊維、布帛
、スポンジ、発泡体、多孔質膜、焼結体等の多孔質体；
粉末、顆粒、ペレット、錠剤等であっても、あるいはそ
れらのものを支持テープ等の特定のベースに取り付けた
もの、容器やチューブ等に充填したもの等のいずれでも
よい。[0013] Although the above explanation has been given using urinary occult blood and glucose as examples, the color test device of the present invention is not limited to a color test device used for testing urinary occult blood or glucose. . That is, the color test device as used in the present invention can be used by any means such as dipping, dropping, injecting, spraying, or applying a liquid to be tested such as human or animal urine, blood, bone marrow fluid, sweat, tears, or saliva. When applied to a color test device, the color changes depending on the presence and concentration of specific substances contained in the test liquid, such as occult blood, glucose, protein, urobulinogen, ketone bodies, bisalvin, pH, and nitrite. This includes any test device that develops or changes color to determine the presence or absence and concentration of these substances. Color test devices are usually in the form of a test piece in which filter paper is impregnated or supported with a specific reagent or enzyme that reacts with each substance to be tested, or a test piece is attached to a base material such as plastic tape. Although often used, the color test device referred to in the present invention may be of any material, form, shape, size, etc., and is not particularly limited. For example, the color test device includes, in addition to a test piece or test paper, a porous body such as a fiber, cloth, sponge, foam, porous membrane, or sintered body of any shape and size;
It may be a powder, granule, pellet, tablet, etc., or it may be attached to a specific base such as a support tape, or filled into a container, tube, etc.
【００１４】そして、判定作業、特に光学読取り機械に
よる判定作業を容易にし、且つ目視および光学読取り機
械の両方で正確で且つ同じ判定結果を得るためには、本
発明の標準色試験体は、呈色試験具と同じかまたは類似
した素材、形状、構造、形態とするのがよい。しかしな
がら、それには限定されず、呈色試験具と素材、形状、
構造、形態等が異なっていてもよい。本発明の標準色試
験体の調製に当たっては、濃度既知の実検体または疑似
検体により呈色させた呈色試験具の光吸収特性を光学装
置を使用して測定し、測定された呈色試験具の光吸収特
性と同じかまたは近似した光吸収特性になるように色素
およびその他の光吸収性物質を選択し、必要に応じてそ
れらの複数を組み合わせて着色用材料を調合する。この
調合された着色用材料を、濾紙、繊維、布帛、スポンジ
、発泡体、多孔質膜、焼結体等の多孔質体や粉末、顆粒
、ペレット、錠剤等の任意の材料に混合、浸漬、滴下、
注入、散布、塗布等の任意の手段を使用して施し、必要
に応じて乾燥処理等を行って標準色試験体を形成する。[0014] In order to facilitate the judgment work, especially the judgment work by an optical reading machine, and to obtain accurate and identical judgment results both visually and by the optical reading machine, the standard color test specimen of the present invention has the following characteristics: It is recommended that the material, shape, structure, and form be the same as or similar to the color test device. However, it is not limited to this, and includes color test tools, materials, shapes, etc.
The structures, shapes, etc. may be different. In preparing the standard color test material of the present invention, the light absorption characteristics of a color test device colored with an actual sample or a pseudo sample of known concentration are measured using an optical device, and the measured color test device is Pigments and other light-absorbing substances are selected so that they have light absorption properties that are the same as or similar to those of the coloring material, and a plurality of them are combined as necessary to prepare a coloring material. This mixed coloring material is mixed into a porous body such as filter paper, fiber, cloth, sponge, foam, porous membrane, sintered body, etc., or any material such as powder, granule, pellet, tablet, etc., dipped, dripping,
It is applied using any method such as injection, spraying, coating, etc., and if necessary, a drying treatment is performed to form a standard color test specimen.
【００１５】標準色試験体を調製するのに使用される色
素およびその他の光吸収性物質としては、安定なものを
使用することが必要であり、それによって標準色試験体
の色や光吸収特性を長期に亙って変化することなく安定
に保つことができる。色素およびその他の光吸収性物質
としては、呈色試験具の呈する色に合わせて、従来この
種の標準色試験体において用いられてきた種々の色素、
赤外線領域に吸収特性を有する物質（赤外線吸収剤等）
、紫外線領域に吸収特性を有する物質（紫外線吸収剤等
）を、各々の標準色試験体に応じて、単独でまたは組み
合わせて使用するのがよい。It is necessary that the dyes and other light-absorbing substances used to prepare the standard color specimens be stable, so that the color and light absorption properties of the standard color specimens are can be kept stable over a long period of time without changing. As pigments and other light-absorbing substances, various pigments that have been conventionally used in this type of standard color test material can be used, depending on the color exhibited by the color test device.
Substances that have absorption properties in the infrared region (infrared absorbers, etc.)
It is preferable to use substances having absorption properties in the ultraviolet region (such as ultraviolet absorbers) either singly or in combination depending on each standard color test specimen.
【００１６】本発明における標準色試験体の例としては
、上記した潜血試験片およびブドウ糖試験片用の標準色
試験具の他に、蛋白質試験片、ウロビリノーゲン試験片
、ケトン体試験片、ビリルビン試験片、ｐＨ試験片、亜
硝酸試験片等を挙げることができる。Examples of standard color test pieces in the present invention include, in addition to the above-mentioned standard color test pieces for occult blood test pieces and glucose test pieces, protein test pieces, urobilinogen test pieces, ketone body test pieces, and bilirubin test pieces. , pH test piece, nitrous acid test piece, etc.
【００１７】[0017]
実施例 １
現在市販されている潜血検査用の潜血試験片［テルモ（
株）製ウリエースＭＮ］およびブドウ糖検査用のブドウ
糖試験片［テルモ（株）製ウリエ−スＭＮ］の各々を、
潜血濃度０．７５ｍｇ／ｄｌ、ブドウ糖濃度５００ｍｇ
／ｄｌに予め調整した濃度既知の尿中に浸漬して青色に
呈色させた。そして、それらの光吸収特性を大塚電子製
の光学読取り機ＭＣＰＤ−２００を使用して測定したと
ころ、潜血試験片の光吸収特性は図１に示すとおりであ
り、またブドウ糖試験片の光吸収特性は図２に示すとお
りであった。Example 1 Currently commercially available occult blood test strip for occult blood testing [Terumo (
[Uriese MN manufactured by Terumo Corporation] and a glucose test piece for glucose testing [Uriese MN manufactured by Terumo Corporation],
Occult blood concentration 0.75 mg/dl, glucose concentration 500 mg
The sample was immersed in urine of known concentration, which had been adjusted in advance to /dl, to develop a blue color. When their light absorption characteristics were measured using an optical reader MCPD-200 manufactured by Otsuka Electronics, the light absorption characteristics of the occult blood test piece were as shown in Figure 1, and the light absorption characteristics of the glucose test piece were measured. was as shown in Figure 2.
【００１８】また、これらの試験片に対する目視用の標
準色試験片の製造に際して常用されている、アミドブラ
ック１０Ｂの０．０１％水溶液を濾紙に含浸し乾燥して
上記潜血試験片およびブドウ糖試験片と同じ青色を呈す
る標準色試験体を製造した。この標準色試験体の光吸収
特性を上記した光学読取り機ＭＣＰＤ−２００を使用し
て測定したところ、図３に示すとおりであった。図３で
は、４００ｎｍ〜７００ｎｍの可視光線領域では、上記
潜血試験片およびブドウ糖試験片との光吸収特性と近似
しているものの、７００ｎｍ〜９００ｎｍの波長領域に
は吸収がなかった。この標準色試験体を用いて光学読取
り機ＭＣＰＤ−２００の動作確認および調整を行った後
に、同じ光学読取り機によって実検体に対して使用した
潜血用呈色試験片およびブドウ糖用呈色試験片の呈色状
態を検査したところ、目視による場合と異なる結果にな
り、潜血およびブドウ糖の有無を明確に判定できなかっ
た。In addition, filter paper was impregnated with a 0.01% aqueous solution of Amido Black 10B, which is commonly used in the production of standard color test pieces for visual inspection of these test pieces, and dried to obtain the above-mentioned occult blood test pieces and glucose test pieces. A standard color specimen exhibiting the same blue color was manufactured. The light absorption characteristics of this standard color test specimen were measured using the optical reader MCPD-200 described above, and the results were as shown in FIG. In FIG. 3, in the visible light range of 400 nm to 700 nm, the light absorption characteristics were similar to those of the occult blood test piece and the glucose test piece, but there was no absorption in the wavelength range of 700 nm to 900 nm. After confirming and adjusting the operation of the optical reader MCPD-200 using this standard color test specimen, the color test strip for occult blood and the color test strip for glucose used for the actual specimen were tested using the same optical reader. When the color state was examined, the results were different from those obtained by visual inspection, and the presence or absence of occult blood and glucose could not be clearly determined.
【００１９】これに対して、アミドブラック１０Ｂを０
．０１％含有し、更に赤外線吸収剤であるＡｌｄｒｉｃ
ｈ社製のＩＲ−１４０を０．２％含有する水溶液を濾紙
に含浸させ、乾燥して本発明の標準色試験体を製造した
。この本発明の標準色試験体の光吸収特性を上記した光
学読取り機ＭＣＰＤ−２００を使用して測定したところ
、図５に示すとおりであった。この図５では、４００ｎ
ｍ〜９００ｎｍのすべての波長領域において上記潜血試
験片およびブドウ糖試験片と極めて近似した光吸収特性
を有していた。この標準色試験体を用いて光学読取り機
ＭＣＰＤ−２００の動作確認および調整を行った後に、
同じ光学読取り機によって実検体に対して使用した潜血
用呈色試験片およびブドウ糖用呈色試験片の呈色状態を
検査したところ、目視による場合と同じ結果になり、潜
血およびブドウ糖の有無を明確に判定できた。On the other hand, amide black 10B was added to 0
．． 01% and further contains Aldric, which is an infrared absorber.
A filter paper was impregnated with an aqueous solution containing 0.2% of IR-140 manufactured by Company H and dried to produce a standard color test specimen of the present invention. The light absorption characteristics of this standard color test specimen of the present invention were measured using the optical reader MCPD-200 described above, and the results were as shown in FIG. In this Figure 5, 400n
It had light absorption characteristics extremely similar to those of the occult blood test piece and the glucose test piece in the entire wavelength range from m to 900 nm. After confirming and adjusting the operation of the optical reader MCPD-200 using this standard color test specimen,
When the coloring status of the occult blood coloring test piece and the glucose coloring test piece used for the actual specimen were examined using the same optical reader, the results were the same as those obtained by visual inspection, and the presence or absence of occult blood and glucose was clearly determined. I was able to determine that.
【００２０】[0020]
【発明の効果】本発明の標準色試験体は、種々の光学読
取り機に対して互換性があり、それらの動作確認および
調整のために有効に使用できる。更に、本発明の標準色
試験体は、目視による標準色試験体としても使用でき、
光学読取り機による場合と目視による場合とで正確で且
つ同じ判定結果を得ることができる。また、本発明の標
準色試験体は、その呈色状態および光吸収特性が安定に
維持され変化しないために、長期間に亙って使用できる
。Effects of the Invention The standard color test specimen of the present invention is compatible with various optical readers and can be effectively used for checking and adjusting their operations. Furthermore, the standard color test specimen of the present invention can also be used as a standard color test specimen by visual inspection.
Accurate and identical determination results can be obtained by using an optical reader and by visual inspection. Further, the standard color test specimen of the present invention can be used for a long period of time because its coloring state and light absorption characteristics are stably maintained and do not change.
【図１】市販の潜血検査用の試験片を潜血濃度既知の尿
中に浸漬して青色に呈色させたものの光吸収特性を示す
図である。FIG. 1 is a diagram showing the light absorption characteristics of a commercially available test piece for occult blood testing that is immersed in urine with a known concentration of occult blood and colored blue.
【図２】市販のブドウ糖検査用の試験片をブドウ糖濃度
既知の尿中に浸漬して青色に呈色させたものの光吸収特
性を示す図である。FIG. 2 is a diagram showing the light absorption characteristics of a commercially available test piece for glucose testing that was immersed in urine of known glucose concentration to turn blue.
【図３】図１および図２の光吸収特性を有する潜血試験
片およびブドウ糖試験片に対して用いられているアミド
ブラック１０Ｂ色素を使用して得られた目視用の標準色
試験体の光吸収特性を示す図である。FIG. 3: Light absorption of standard color specimens for visual inspection obtained using Amido Black 10B dye used for occult blood test strips and glucose test strips having the light absorption characteristics shown in FIGS. 1 and 2. FIG. 3 is a diagram showing characteristics.
【図４】図１および図２の光吸収特性を有する潜血試験
片およびブドウ糖試験片に対して用いられているＰＡＮ
ＴＯＮＥ社製の３６０Ｕ色素を使用して得られた目視用
の標準色試験体の光吸収特性を示す図である。FIG. 4: PAN used for occult blood test strips and glucose test strips having the light absorption characteristics of FIGS. 1 and 2.
It is a figure which shows the light absorption characteristic of the standard color test piece for visual observation obtained using 360U dye manufactured by TONE.
【図５】図１および図２の光吸収特性を有する潜血試験
片およびブドウ糖試験片と近似した光吸収特性を有する
ように調製された本発明の標準色試験体の光吸収特性を
示す図である。FIG. 5 is a diagram showing the light absorption characteristics of the standard color test specimen of the present invention prepared to have light absorption characteristics similar to those of the occult blood test piece and the glucose test piece shown in FIGS. 1 and 2. be.
Claims (2)
り呈色させた呈色試験具の光吸収特性と同じかまたは近
似した光吸収特性を有するように色素および／またはそ
の他の特定の波長領域に光吸収特性を有する物質を担持
させてなることを特徴とする標準色試験体。Claim 1: Pigment and/or other specific wavelength range so as to have light absorption characteristics that are the same as or similar to those of a color test device colored by an actual sample or a pseudo sample of known concentration. A standard color test specimen characterized by supporting a substance having light absorption characteristics.
り呈色させた呈色試験具の光吸収特性を測定し、この光
吸収特性と同じかまたは近似した光吸収特性になるよう
に色素および／またはその他の特定の波長領域に光吸収
特性を有する物質を調合し、この調合物を使用して請求
項１の標準色試験体を製造する方法。2. Measure the light absorption properties of a color test device colored with an actual sample or a pseudo sample of known concentration, and add a dye and/or so that the light absorption properties are the same as or similar to the measured light absorption properties. A method for producing the standard color test specimen according to claim 1 by preparing a substance having light absorption characteristics in a specific wavelength range or by using this preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5374691A JPH04339257A (en) | 1991-02-27 | 1991-02-27 | Standard color testing body and manufacture thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5374691A JPH04339257A (en) | 1991-02-27 | 1991-02-27 | Standard color testing body and manufacture thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04339257A true JPH04339257A (en) | 1992-11-26 |
Family
ID=12951381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5374691A Pending JPH04339257A (en) | 1991-02-27 | 1991-02-27 | Standard color testing body and manufacture thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04339257A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001194368A (en) * | 1999-12-17 | 2001-07-19 | Bayer Corp | Improved test piece for identifying analyte in liquid sample |
-
1991
- 1991-02-27 JP JP5374691A patent/JPH04339257A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001194368A (en) * | 1999-12-17 | 2001-07-19 | Bayer Corp | Improved test piece for identifying analyte in liquid sample |
| JP2010160164A (en) * | 1999-12-17 | 2010-07-22 | Bayer Corp | Improved test strip for assay of analyte in liquid sample |
| JP4571300B2 (en) * | 1999-12-17 | 2010-10-27 | バイエルコーポレーション | Improved test strip for the assay of analytes in liquid samples |
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