JPH04335839A - Method for promoting morphogenesis or differentiation of plant - Google Patents
Method for promoting morphogenesis or differentiation of plantInfo
- Publication number
- JPH04335839A JPH04335839A JP13217291A JP13217291A JPH04335839A JP H04335839 A JPH04335839 A JP H04335839A JP 13217291 A JP13217291 A JP 13217291A JP 13217291 A JP13217291 A JP 13217291A JP H04335839 A JPH04335839 A JP H04335839A
- Authority
- JP
- Japan
- Prior art keywords
- differentiation
- plant
- oligosaccharide
- acid
- morphogenesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000004069 differentiation Effects 0.000 title claims abstract description 24
- 230000019552 anatomical structure morphogenesis Effects 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title claims abstract description 8
- 230000001737 promoting effect Effects 0.000 title claims description 6
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 30
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 19
- 229920000615 alginic acid Polymers 0.000 claims abstract description 19
- -1 alginic acid oligosaccharide Chemical class 0.000 claims abstract description 17
- 239000000783 alginic acid Substances 0.000 claims abstract description 6
- 229960001126 alginic acid Drugs 0.000 claims abstract description 6
- 229940072056 alginate Drugs 0.000 claims description 13
- 150000002482 oligosaccharides Chemical class 0.000 claims description 12
- AEMOLEFTQBMNLQ-VANFPWTGSA-N D-mannopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-VANFPWTGSA-N 0.000 claims description 8
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 8
- AEMOLEFTQBMNLQ-BZINKQHNSA-N D-Guluronic Acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@@H](O)[C@H]1O AEMOLEFTQBMNLQ-BZINKQHNSA-N 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 7
- AEMOLEFTQBMNLQ-UHFFFAOYSA-N beta-D-galactopyranuronic acid Natural products OC1OC(C(O)=O)C(O)C(O)C1O AEMOLEFTQBMNLQ-UHFFFAOYSA-N 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 4
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 13
- 210000000056 organ Anatomy 0.000 abstract description 8
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 210000002242 embryoid body Anatomy 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 23
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- 206010020649 Hyperkeratosis Diseases 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 239000006870 ms-medium Substances 0.000 description 5
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000661 sodium alginate Substances 0.000 description 4
- 235000010413 sodium alginate Nutrition 0.000 description 4
- 229940005550 sodium alginate Drugs 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000234435 Lilium Species 0.000 description 3
- 241000208125 Nicotiana Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 108010004131 poly(beta-D-mannuronate) lyase Proteins 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】
【0001】
【産業上の利用分野】本発明は、植物の形態形成又は分
化の促進方法に関し、詳しくは植物の組織又は細胞から
新たに植物の器官又は胚様体を形成させる特定の形質を
保有する植物を選定、維持し、これを種苗化するに際し
、該植物の組織又は細胞の培養基中に特定の成分を加え
ることにより、植物の器官又は胚様体の形成を促進させ
、農業生産上有用な種苗の生産を効率的に行うことを目
的とするものである。
【0002】
【従来の技術及び発明が解決しようとする課題】植物種
苗の改良は、農業における重要な課題であり、古来より
精力的に取り組まれている。近年になって、遺伝子工学
の進歩や細胞融合技術の発展、さらには組織培養技術,
ロボット工学の進歩など先端技術が実用化の段階に入り
、このような先端技術を組み合わせた、いわゆるバイオ
ナーサリーシステムによる農園芸用有用植物種苗の生産
が開始されている。
【0003】このような種苗生産方法において最も重要
な工程は、目的とする植物の組織や細胞を培養し、新た
な器官等を形成又は分化させることにある。従来から、
培地組成,温度,pH等のほか、オーキシン,カイネチ
ン等の植物ホルモン類の施用濃度,添加時期等の諸条件
が検討されてきた。しかしながら、このような検討の結
果、設定された条件下でも、器官等の形成率,分化の頻
度は実用的に十分とは言えず、植物の器官等の形成率や
分化を促進する手段の開発が待望されていた。
【0004】
【課題を解決するための手段】本発明の目的は、植物の
器官等の形成率や分化を促進する物質を見出し、該物質
を植物種苗の生産に応用して生産の効率化を図ることで
ある。発明者らは、かかる物質を検索すべく鋭意検討を
加えた結果、アルギン酸オリゴ糖が有効であることを見
出し、本発明に到達したのである。すなわち、本発明は
植物の組織又は細胞を培養するに際し、培地中にアルギ
ン酸オリゴ糖若しくはその加熱処理物を加えることを特
徴とする植物の形態形成又は分化の促進方法を提供する
ものである。
【0005】本発明に用いるアルギン酸オリゴ糖は、ア
ルギン酸,アルギン酸ナトリウム或いはアルギン酸を含
む昆布などの藻類、微生物起源の多糖体などをアルギン
酸リアーゼなどの酵素で分解するか、又は塩酸などの酸
で加水分解して得られるオリゴ糖組成物である。オリゴ
糖の構成成分としては、グルロン酸,マンヌロン酸及び
4,5−デオキシウロン酸が主成分である。オリゴ糖組
成物とは、その重合度が2〜20であり、グルロン酸及
び/又はマンヌロン酸或いはこれらと4,5−デオキシ
ウロン酸を構成成分とするオリゴ糖からなる組成物、若
しくはこれらを加熱処理したものを意味し、具体的には
グルロン酸のみで構成されるオリゴ糖、マンヌロン酸の
みで構成されるオリゴ糖、グルロン酸と4,5−デオキ
シウロン酸で構成されるオリゴ糖、グルロン酸とマンヌ
ロン酸で構成されるオリゴ糖、マンヌロン酸と4,5−
デオキシウロン酸で構成されるオリゴ糖、グルロン酸,
マンヌロン酸及び4,5−デオキシウロン酸の3者で構
成されるオリゴ糖、若しくはこれらをpH1〜8、温度
90〜130℃の条件下で15〜180分加熱処理して
得られるものを挙げることができる。
【0006】次に、本発明が適用される植物については
特に制限されず、例えばジャガイモ,トマト,キャベツ
,人参,レタス等の野菜類、稲,小麦,トウモロコシ等
の穀類、ユリ等の花卉類、ミカン,リンゴ等の果樹類等
広く適用することができる。
【0007】アルギン酸オリゴ糖若しくはその加熱処理
物は、植物の組織又は細胞の培養に用いる培地に通常0
.01〜5000ppmの濃度で添加して用いられるが
、別の態様として本物質を含有する液中に植物の組織又
は細胞を浸漬したり、該液を植物の組織又は細胞に直接
塗布したりして用いることも可能である。
【0008】アルギン酸オリゴ糖若しくはその加熱処理
物を使用すること以外は、既知の条件により植物の組織
又は細胞を培養し、目的とする植物の形態形成又は分化
を行えばよい。
【0009】
【実施例】次に、本発明を実施例により説明するが、本
発明はこれらにより制限されるものではない。
実施例1
アルギン酸オリゴ糖の製造
アルギン酸ソーダ1部につき水を10部加えて溶解後、
pHを7.0に調整し、アルギン酸ソーダ1g当りアル
ギン酸リアーゼ3000単位を添加し、酵素反応を行っ
た。なお、アルギン酸リアーゼの酵素活性はpH7.0
,30℃で0.2%アルギン酸ソーダ溶液に酵素を作用
させたとき、30分間に230nmの吸光度を0.01
上昇させる酵素力を1単位とした。酵素反応は30℃で
24時間行い、反応後さらにpHを3.0に調整して1
00℃で60分間の加熱処理を行った。以上の操作によ
り、重合度2〜20のオリゴ糖を固形分当り70%以上
含むアルギン酸オリゴ糖を得ることができた。
【0010】実施例2
ジャガイモマイクロチューバー
茎頂培養により増殖したジャガイモのシュートを用い、
マイクロチューバーの形成数に及ぼすアルギン酸オリゴ
糖の影響について検討した。
【0011】シュートを3%シュークロースと0.8%
寒天を含むMurashige−Skoog 培地(以
下、MS培地と称する。)で増殖させた後、5%シュー
クロース,5ppmベンジルアデニン,0.8%寒天及
び各種濃度の実施例1で製造したアルギン酸オリゴ糖を
含むMS培地でマイクロチューバーを誘導した。培養1
ヵ月後の直径3mm以上のマイクロチューバー形成数と
生重量を表1に示す。なお、表中の()は対照に対する
指数である。
【0012】
表 1 濃度(ppm) チューバー形成
数(個/lot) 全生重量(g)
0 10 (100)
3.1 (100
) 6
11 (110)
3.3 (106) 60
19 (190)
5.2 (168)
600 18 (180)
4.3 (139)
【0013】実施例3
ユリ鱗片培養
スカシユリの鱗片から誘導したバルブレットを培養し、
茎葉分化に及ぼすアルギン酸オリゴ糖の影響について検
討した。
【0014】バルブレットを3%シュークロース及び0
.8%寒天を含むMS培地で誘導し、5mm角の切片と
した。この切片を3%シュークロース,0.1%カイネ
チン,0.1ppmNAA,0.8%寒天及び各種濃度
の実施例1で製造したアルギン酸オリゴ糖を含むMS培
地で培養した。培養2ヵ月後の茎葉分化数を表2に示し
た。なお、表中の( )は対照に対する指数である。
【0015】
表 2 濃度
(ppm) 茎葉分化数(本/lot)
0
2 ( 100)
0.025
12 ( 600)
2.5
24 (1200)
250 29
(1450) 【0016】実施例4
タバコカルスの茎葉分化
タバコカルスの茎葉分化に及ぼすアルギン酸オリゴ糖の
影響について検討した。タバコ(Nicotiana
tabacum)のカルスを増殖用培地(1.0ppm
NAA,0.1ppmBA及び0.8%寒天を含むMS
培地)で培養し、継代20日目のカルスを茎葉分化に用
いた。
【0017】カルスを約1cm角の切片とし、分化用培
地(0.1ppmNAA,1.0ppmBA,0.8%
寒天及び各種濃度の実施例1で製造したアルギン酸オリ
ゴ糖を含むMS培地)で約1ヵ月培養し、茎葉分化数を
比較した。結果を表3に示すが、数値は各区10片のカ
ルスを用い、カルス当りの茎葉分化数で示したものであ
る。なお、表中の( )は対照に対する指数である。
【0018】
表 3 濃度(ppm
) 茎葉分化数(本/lot)
0
1.6 (100)
0.1
3.1 (194)
1.0 3.9
(244)
10 4.7 (29
4) 100
4.5 (281)
1000
4.4 (275)
5000
4.1 (256) 【0019】
【発明の効果】植物の組織又は細胞を培養して新たな器
官等を形成又は分化させるに際して、培地中にアルギン
酸オリゴ糖若しくはその加熱処理物を添加することによ
り、植物の形態形成並びに分化を促進して有用な農園芸
作物の人工種苗の生産効率を向上させることができる。[0001] The present invention relates to a method for promoting morphogenesis or differentiation of plants, and more specifically, the present invention relates to a method for promoting morphogenesis or differentiation of plants, and more specifically, the present invention relates to a method for promoting morphogenesis or differentiation of plants, and more specifically, for producing new plant organs or embryoid bodies from plant tissues or cells. When selecting and maintaining plants that possess specific traits that cause them to form, and when producing them as seedlings, it is possible to enhance the formation of plant organs or embryoid bodies by adding specific components to the culture medium of the plant's tissues or cells. The purpose is to promote the efficient production of seeds and seedlings useful in agricultural production. BACKGROUND OF THE INVENTION [0002] Improvement of plant seeds and seedlings is an important issue in agriculture, and has been energetically tackled since ancient times. In recent years, advances in genetic engineering, cell fusion technology, tissue culture technology,
Advanced technologies such as advances in robotics have entered the stage of practical application, and the production of useful plant seeds and seedlings for agriculture and horticulture has begun using so-called bionursery systems that combine such advanced technologies. The most important step in such seedling production methods is to culture target plant tissues and cells to form or differentiate new organs and the like. Traditionally,
In addition to medium composition, temperature, pH, etc., various conditions such as application concentration and addition timing of plant hormones such as auxin and kinetin have been investigated. However, as a result of these studies, even under the set conditions, the rate of organ formation and the frequency of differentiation are not sufficient for practical purposes, and the development of means to promote the rate of formation and differentiation of plant organs, etc. was long awaited. [Means for Solving the Problems] An object of the present invention is to discover a substance that promotes the formation rate and differentiation of plant organs, and to apply this substance to the production of plant seeds and seedlings to improve production efficiency. It is to aim for it. As a result of extensive research to search for such substances, the inventors discovered that alginic acid oligosaccharides are effective, and have arrived at the present invention. That is, the present invention provides a method for promoting plant morphogenesis or differentiation, which comprises adding alginate oligosaccharide or a heat-treated product thereof to a medium when culturing plant tissues or cells. [0005] The alginate oligosaccharide used in the present invention can be obtained by decomposing alginic acid, sodium alginate, alginate-containing algae such as kelp, or microbial polysaccharide with an enzyme such as alginate lyase, or hydrolyzing with an acid such as hydrochloric acid. This is an oligosaccharide composition obtained by The main components of the oligosaccharide are guluronic acid, mannuronic acid, and 4,5-deoxyuronic acid. An oligosaccharide composition is a composition having a degree of polymerization of 2 to 20 and consisting of an oligosaccharide containing guluronic acid and/or mannuronic acid, or these and 4,5-deoxyuronic acid, or a composition in which these are heated. Processed products, specifically oligosaccharides composed only of guluronic acid, oligosaccharides composed only of mannuronic acid, oligosaccharides composed of guluronic acid and 4,5-deoxyuronic acid, and guluronic acid. An oligosaccharide composed of mannuronic acid and mannuronic acid, mannuronic acid and 4,5-
Oligosaccharide composed of deoxyuronic acid, guluronic acid,
Oligosaccharides composed of mannuronic acid and 4,5-deoxyuronic acid, or those obtained by heat-treating these at pH 1-8 and temperature 90-130°C for 15-180 minutes. Can be done. Next, the plants to which the present invention is applied are not particularly limited, and include vegetables such as potatoes, tomatoes, cabbage, carrots, and lettuce, grains such as rice, wheat, and corn, and flowers such as lilies. It can be widely applied to fruit trees such as mandarin oranges and apples. Oligosaccharide alginate or its heat-treated product is usually added to the medium used for culturing plant tissues or cells.
.. It is used by adding it at a concentration of 0.01 to 5000 ppm, but in another embodiment, the plant tissue or cells are immersed in a solution containing this substance, or the solution is directly applied to the plant tissue or cells. It is also possible to use [0008] Except for using alginic acid oligosaccharide or a heat-treated product thereof, plant tissues or cells may be cultured under known conditions to achieve morphogenesis or differentiation of the desired plant. [Example] Next, the present invention will be explained with reference to Examples, but the present invention is not limited thereto. Example 1 Production of alginate oligosaccharide After dissolving 10 parts of water per 1 part of sodium alginate,
The pH was adjusted to 7.0, and 3000 units of alginate lyase was added per 1 g of sodium alginate to perform an enzyme reaction. In addition, the enzymatic activity of alginate lyase is at pH 7.0.
, When the enzyme was applied to a 0.2% sodium alginate solution at 30°C, the absorbance at 230 nm decreased to 0.01 in 30 minutes.
The enzyme power to be increased was defined as 1 unit. The enzyme reaction was carried out at 30°C for 24 hours, and after the reaction, the pH was further adjusted to 3.0.
Heat treatment was performed at 00°C for 60 minutes. Through the above operations, alginic acid oligosaccharides containing 70% or more of oligosaccharides having a degree of polymerization of 2 to 20 based on the solid content could be obtained. Example 2 Using potato shoots grown by potato microtuber shoot apical culture,
The effect of alginate oligosaccharides on the number of microtubers formed was investigated. [0011] Shoots are mixed with 3% sucrose and 0.8%
After growing in Murashige-Skoog medium (hereinafter referred to as MS medium) containing agar, 5% sucrose, 5 ppm benzyladenine, 0.8% agar and various concentrations of alginate oligosaccharide prepared in Example 1 were grown. Microtubers were induced with MS medium containing Culture 1
Table 1 shows the number of microtubers with a diameter of 3 mm or more formed and the fresh weight after a month. Note that () in the table is an index relative to the control. 0012
Table 1 Concentration (ppm) Number of tubers formed (pieces/lot) Total fresh weight (g)
0 10 (100)
3.1 (100
) 6
11 (110)
3.3 (106) 60
19 (190)
5.2 (168)
600 18 (180)
4.3 (139)
Example 3 Cultivation of lily scales Bulblets derived from lily scales were cultured.
The effect of alginate oligosaccharide on shoot differentiation was investigated. [0014] Bulblet was mixed with 3% sucrose and 0
.. The cells were induced in MS medium containing 8% agar and cut into 5 mm square sections. This section was cultured in MS medium containing 3% sucrose, 0.1% kinetin, 0.1 ppm NAA, 0.8% agar, and various concentrations of the alginate oligosaccharide prepared in Example 1. Table 2 shows the number of stem and leaf differentiation after two months of culture. Note that ( ) in the table is an index relative to the control. [0015]
Table 2 Concentration (ppm) Number of stem/leaf differentiation (plants/lot)
0
2 (100)
0.025
12 (600)
2.5
24 (1200)
250 29
(1450) Example 4 Stem and leaf differentiation of tobacco callus The influence of alginate oligosaccharide on the differentiation of tobacco callus into leaf and stalk was investigated. Tobacco (Nicotiana)
tabacum) in a growth medium (1.0 ppm).
MS containing NAA, 0.1 ppm BA and 0.8% agar
The callus was cultured in a medium (medium), and the calli on the 20th day of subculture were used for differentiation into stems and leaves. [0017] The callus was cut into approximately 1 cm square sections, and a differentiation medium (0.1 ppmNAA, 1.0 ppm BA, 0.8%
The cells were cultured for about one month on MS medium containing agar and alginate oligosaccharide prepared in Example 1 at various concentrations, and the number of stem and leaf differentiation was compared. The results are shown in Table 3, and the values are expressed as the number of stem and leaf differentiation per callus using 10 pieces of callus from each section. Note that ( ) in the table is an index relative to the control. [0018]
Table 3 Concentration (ppm
) Number of stem and leaf differentiation (pcs/lot)
0
1.6 (100)
0.1
3.1 (194)
1.0 3.9
(244)
10 4.7 (29
4) 100
4.5 (281)
1000
4.4 (275)
5000
4.1 (256) [Effects of the Invention] When culturing plant tissues or cells to form or differentiate new organs, etc., by adding alginate oligosaccharide or a heat-treated product thereof to the medium. , it is possible to promote the morphogenesis and differentiation of plants and improve the production efficiency of artificial seeds and seedlings of useful agricultural and horticultural crops.
Claims (2)
、培地中にアルギン酸オリゴ糖若しくはその加熱処理物
を加えることを特徴とする植物の形態形成又は分化の促
進方法。1. A method for promoting plant morphogenesis or differentiation, which comprises adding alginate oligosaccharide or a heat-treated product thereof to a medium when culturing plant tissues or cells.
0の、グルロン酸及び/又はマンヌロン酸或いはこれら
と4,5−デオキシウロン酸を構成成分とするオリゴ糖
である請求項1記載の方法。Claim 2: The alginic acid oligosaccharide has a polymerization degree of 2 to 2.
2. The method according to claim 1, wherein the oligosaccharide is an oligosaccharide whose constituent components are guluronic acid and/or mannuronic acid, or these and 4,5-deoxyuronic acid.
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JP13217291A JP2663408B2 (en) | 1991-05-09 | 1991-05-09 | Method for promoting plant morphogenesis or differentiation |
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JP13217291A JP2663408B2 (en) | 1991-05-09 | 1991-05-09 | Method for promoting plant morphogenesis or differentiation |
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JPH04335839A true JPH04335839A (en) | 1992-11-24 |
JP2663408B2 JP2663408B2 (en) | 1997-10-15 |
Family
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997033593A1 (en) * | 1996-03-15 | 1997-09-18 | Takara Shuzo Co., Ltd. | A product of heat treatment of uronic acid, food, drink or drug including the product |
FR2795289A1 (en) * | 1999-06-25 | 2000-12-29 | Centre Nat Rech Scient | 1,4 Beta-D-glucuronan polymers and their derivatives having an enzyme amplifying effect, are useful as phytosanitary products and biofertilizers |
WO2001040315A1 (en) * | 1999-11-30 | 2001-06-07 | Dalian Yaweite Biology Engineering Co., Ltd. | The alginate having low molecular weight, methods of manufacturing it and its use |
-
1991
- 1991-05-09 JP JP13217291A patent/JP2663408B2/en not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997033593A1 (en) * | 1996-03-15 | 1997-09-18 | Takara Shuzo Co., Ltd. | A product of heat treatment of uronic acid, food, drink or drug including the product |
EA001535B1 (en) * | 1996-03-15 | 2001-04-23 | Такара Сузо Ко., Лтд. | Apoptosis inducing product and method for manufacturing same, apoptosis inducer and method of apoptosis inducing, inducer for differentiation of canceous cells, food product, pharmaceutical compositions and hygienic means |
US6482806B1 (en) | 1996-03-15 | 2002-11-19 | Takara Shuzo Co., Ltd. | Product of heat treatment of uronic acid, food, drink, or drug including the product |
FR2795289A1 (en) * | 1999-06-25 | 2000-12-29 | Centre Nat Rech Scient | 1,4 Beta-D-glucuronan polymers and their derivatives having an enzyme amplifying effect, are useful as phytosanitary products and biofertilizers |
WO2001000025A1 (en) * | 1999-06-25 | 2001-01-04 | Central National De La Recherche Scientifique | Use of glycuronic polysaccharides and oligosaccharides as phytosanitary products and/or fertilisers |
US7112555B1 (en) | 1999-06-25 | 2006-09-26 | Centre National De La Recherche Scientifique | Use of glycuronic polysaccharides and oligosaccharides as phytosanitary products and/or fertiliser |
WO2001040315A1 (en) * | 1999-11-30 | 2001-06-07 | Dalian Yaweite Biology Engineering Co., Ltd. | The alginate having low molecular weight, methods of manufacturing it and its use |
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JP2663408B2 (en) | 1997-10-15 |
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