JPH04169529A - Livestock mastitis-preventing agent and preparation thereof - Google Patents
Livestock mastitis-preventing agent and preparation thereofInfo
- Publication number
- JPH04169529A JPH04169529A JP29080290A JP29080290A JPH04169529A JP H04169529 A JPH04169529 A JP H04169529A JP 29080290 A JP29080290 A JP 29080290A JP 29080290 A JP29080290 A JP 29080290A JP H04169529 A JPH04169529 A JP H04169529A
- Authority
- JP
- Japan
- Prior art keywords
- chitosan
- mastitis
- aqueous solution
- mammilla
- pathogenic bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000004396 mastitis Diseases 0.000 title claims abstract description 18
- 244000144972 livestock Species 0.000 title claims description 10
- 238000002360 preparation method Methods 0.000 title description 2
- 229920001661 Chitosan Polymers 0.000 claims abstract description 41
- 239000004480 active ingredient Substances 0.000 claims abstract description 4
- 230000003449 preventive effect Effects 0.000 claims description 5
- 244000052616 bacterial pathogen Species 0.000 abstract description 12
- 239000007864 aqueous solution Substances 0.000 abstract description 10
- 239000003795 chemical substances by application Substances 0.000 abstract description 10
- 239000011248 coating agent Substances 0.000 abstract description 8
- 238000000576 coating method Methods 0.000 abstract description 8
- 230000002378 acidificating effect Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract description 3
- 239000005708 Sodium hypochlorite Substances 0.000 abstract description 2
- 230000007794 irritation Effects 0.000 abstract description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract description 2
- 241000780272 Mammilla Species 0.000 abstract 4
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 abstract 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 abstract 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229960003260 chlorhexidine Drugs 0.000 abstract 1
- 229940050410 gluconate Drugs 0.000 abstract 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000009545 invasion Effects 0.000 abstract 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract 1
- 229940069328 povidone Drugs 0.000 abstract 1
- 210000002445 nipple Anatomy 0.000 description 19
- 241000283690 Bos taurus Species 0.000 description 10
- 230000000844 anti-bacterial effect Effects 0.000 description 9
- 235000013365 dairy product Nutrition 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000008267 milk Substances 0.000 description 6
- 235000013336 milk Nutrition 0.000 description 6
- 210000004080 milk Anatomy 0.000 description 6
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 3
- 210000000481 breast Anatomy 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- IRPVABHDSJVBNZ-RTHVDDQRSA-N 5-[1-(cyclopropylmethyl)-5-[(1R,5S)-3-(oxetan-3-yl)-3-azabicyclo[3.1.0]hexan-6-yl]pyrazol-3-yl]-3-(trifluoromethyl)pyridin-2-amine Chemical compound C1=C(C(F)(F)F)C(N)=NC=C1C1=NN(CC2CC2)C(C2[C@@H]3CN(C[C@@H]32)C2COC2)=C1 IRPVABHDSJVBNZ-RTHVDDQRSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229920000153 Povidone-iodine Polymers 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 229960003333 chlorhexidine gluconate Drugs 0.000 description 2
- YZIYKJHYYHPJIB-UUPCJSQJSA-N chlorhexidine gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.C1=CC(Cl)=CC=C1NC(=N)NC(=N)NCCCCCCNC(=N)NC(=N)NC1=CC=C(Cl)C=C1 YZIYKJHYYHPJIB-UUPCJSQJSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229960001621 povidone-iodine Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical group OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 125000004183 alkoxy alkyl group Chemical group 0.000 description 1
- 125000004453 alkoxycarbonyl group Chemical group 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 125000006350 alkyl thio alkyl group Chemical group 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 125000003710 aryl alkyl group Chemical group 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000012993 chemical processing Methods 0.000 description 1
- FLASNYPZGWUPSU-SICDJOISSA-N chitosan Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)N)O[C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)N)O[C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)N)O[C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)N)O[C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)N)O[C@H]1[C@H](O)[C@H]([C@@H](O[C@@H]1CO)O[C@@H]1[C@H](O[C@@H](O[C@@H]2[C@H](O[C@@H](O)[C@H](N)[C@H]2O)CO)[C@H](N)[C@H]1O)CO)NC(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1N FLASNYPZGWUPSU-SICDJOISSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は家畜の***炎予防剤に関する。[Detailed description of the invention] (Industrial application field) The present invention relates to a mastitis preventive agent for livestock.
さらに詳しくは、乳牛などの家畜の乳頭に抗菌性、殺菌
性を有するキトサン及び／またはキトサン誘導体の被膜
を形成することによる***炎の予防剤に関する。More specifically, the present invention relates to an agent for preventing mastitis by forming a coating of chitosan and/or chitosan derivatives having antibacterial and bactericidal properties on the teats of livestock such as dairy cows.
（従来技術）
乳牛などの家畜動物の***炎は、 ５ｔａｐｈｙｌｏｃ
ｏｃｃｕｓａｕｒｅｕｓ、５ｔｒｅｐｔｃｏｃｃｕｓ
ａｇａｌａｃｔｉａｅ、Ｅｓｈｅｒｉｃｈｉａ Ｃｏ１
１その他の病原菌が乳頭口より***内に侵入し、炎症を
起こす疾病である。(Prior art) Mastitis in livestock animals such as dairy cows is treated with 5taphyloc.
occusaureus, 5treptcoccus
agalactiae, Escherichia Co1
1. It is a disease in which other pathogenic bacteria invade the breast through the nipple orifice and cause inflammation.
とりわけ酪農経営者にとっては７本疾病は発生率も高く
、乳量の減少、乳質の低下、供用年数の短縮さらには乳
汁の細菌汚染による公衆衛生上の問題や体細胞の増加に
る乳製品の加工の際の歩止まりの悪化などの大きな損失
をもたらす重大な疾病となっている。For dairy farmers in particular, the incidence of these seven diseases is high, leading to a decrease in milk yield, deterioration in milk quality, shortening of service life, public health problems due to bacterial contamination of milk, and increase in somatic cells in dairy products. It has become a serious disease that causes large losses such as poor yield during processing.
***炎はこのように家畜の重大な疾病であるが、従来の
予防としては、家畜の適切な飼育管理による病原菌に対
する抵抗力の増強や搾乳を清潔に行うなどの原始的な手
段や、ポビドンヨード（ＰＶＰヨード）、グルコン酸ク
ロルヘキシジンなどを含む乳頭浸漬剤（デイツプ）で乳
頭を消毒（ディッピング）する方法などが知られている
が、***炎の発生率は低下してきたものの完全には予防
できていない。また、これらの乳頭浸漬剤は強い殺菌力
を有するものの乳頭への刺激が強くひび割れができたり
、水によって容易に流されるため長時間にわたって殺菌
力が維持されないなどの問題がある。その他、乳頭を外
部環境の病原菌と遮断する被膜を形成するタイプの乳頭
浸漬剤も使用されているが、それ自体殺菌力はなく搾乳
中の病原菌の感染は防げない。Mastitis is thus a serious disease of livestock, but conventional methods of prevention include primitive measures such as increasing the resistance of livestock to pathogenic bacteria through proper breeding management, ensuring clean milking, and using povidone-iodine ( Methods such as disinfecting (dipping) the nipples with teat dipping agents (dip) containing PVP iodine), chlorhexidine gluconate, etc. are known, but although the incidence of mastitis has decreased, it has not been completely prevented. . Furthermore, although these teat immersion agents have a strong bactericidal effect, they have problems such as strong irritation to the teat, causing cracks, and being easily washed away by water, so that the bactericidal effect is not maintained over a long period of time. Other types of teat soaking agents are also used, which form a film that shields the teat from pathogenic bacteria in the external environment, but this agent itself has no bactericidal properties and cannot prevent infection by pathogenic bacteria during milking.
従って、適当な殺菌力を有し乳頭への刺激がなくかつ長
時間病原菌の感染を防ぐ安定な被膜を形成するタイプの
***炎予防剤の開発が望まれている。Therefore, it is desired to develop a mastitis preventive agent that has appropriate bactericidal activity, does not irritate teats, and forms a stable film that prevents infection by pathogenic bacteria for a long period of time.
（発明が解決しようとする課題）
本発明は、乳牛など家畜動物の乳頭に被膜を形成せしめ
、乳頭の病原菌の増殖を抑制し、かつ病原菌の***への
侵入を阻止しうる新しい***炎予防剤を提供することを
目的とする。(Problems to be Solved by the Invention) The present invention provides a new mastitis preventive agent that forms a coating on the teats of livestock animals such as dairy cows, suppresses the growth of pathogenic bacteria on the teats, and prevents pathogenic bacteria from entering the udder. The purpose is to provide
（課題を解決するための手段） 本発明者らは、上記課題を解決すべ（研究した結果。(Means for solving problems) The present inventors should solve the above problems (results of research).
キトサン及び／又はキトサン誘導体が抗菌力を有し乳頭
への刺激がなくかつ良好な被膜を形成することを見出し
本発明を完成させた。The present invention was completed by discovering that chitosan and/or chitosan derivatives have antibacterial activity, do not irritate the nipple, and form a good coating.
本発明の***炎予防剤は、キトサン及び／又はキトサン
誘導体を有効成分とするものである。The mastitis preventive agent of the present invention contains chitosan and/or a chitosan derivative as an active ingredient.
これまで、キトサン及び／又はキトサン誘導体は抗菌性
、殺菌性、創傷治癒作用、免疫賦活性化作用などの種々
の機能を有し、又生体適合性の高い被膜を形成するため
、医薬、化粧品、工業、農業７食品の分野で使用が知ら
れている。Until now, chitosan and/or chitosan derivatives have been used in pharmaceuticals, cosmetics, It is known for use in the industrial, agricultural and food sectors.
本発明は、上記のキトサン及び／又はキトサン誘導体の
すぐれた特徴に着目して、鋭意研究の結果、乳牛などの
家畜の***炎の予防にキトサン及び／又はキトサン誘導
体の水溶液もしくは酸性水溶液をスプレー又は浸漬する
ことかきわめて有効であることを見出した。The present invention focuses on the excellent characteristics of chitosan and/or chitosan derivatives described above, and as a result of intensive research, the present invention has been made by spraying or using an aqueous solution or an acidic aqueous solution of chitosan and/or chitosan derivatives to prevent mastitis in livestock such as dairy cows. I found that immersion was extremely effective.
本発明に用いられるキトサンは、カニ等の甲殻類。The chitosan used in the present invention is obtained from crustaceans such as crabs.
かぶと虫等の昆虫類の甲殻の形成成分である。キチン（
Ｎ−アセチル−Ｄ−グルコサミンの多糖類）が脱アセチ
ル化されたもので、遊離アミノ基を有する塩基性多糖類
高分子物質であり９分子量に特に限定はない。It is a forming component of the shells of insects such as beetles. Kitchen(
N-acetyl-D-glucosamine (polysaccharide) is deacetylated, and is a basic polysaccharide polymer substance having free amino groups, and there is no particular limitation on the molecular weight.
また、脱アセチル化されていない原料であるキチンや化
学処理によって生成する軽度分解物などの不純物を若干
量含有するものも含まれる。It also includes those containing a small amount of impurities such as chitin, which is a raw material that has not been deacetylated, and mild decomposition products produced by chemical processing.
本発明に用いられるキトサン誘導体としては、キトサン
の遊離のアミン基または水酸基をすべであるいは部分的
にアルキル基、アルコキシアルキル基、アルキルチオア
ルキル基、アリール基、アラルキル基、アシル基、スル
ホニル基、アルコキシカルボニル基またはリン酸残基等
により化学修飾されたものを挙げる事ができる。これら
のキトサン誘導体は９例えば以下の文献記載の手法など
の９通常の有幾合成化学的な化学修飾によって得ること
が出来る。The chitosan derivatives used in the present invention include those in which the free amine group or hydroxyl group of chitosan is completely or partially replaced with an alkyl group, an alkoxyalkyl group, an alkylthioalkyl group, an aryl group, an aralkyl group, an acyl group, a sulfonyl group, or an alkoxycarbonyl group. Examples include those chemically modified with groups or phosphoric acid residues. These chitosan derivatives can be obtained by a number of conventional synthetic chemical modifications, such as techniques described in the literature below.
（１）公開特許公報 昭５７−１８０６０２（２）公開
特許公報 昭５９−１０６４０９本発明においてキトサ
ン及び／又はキトサン誘導体は、水溶液あるいは有機酸
または無機酸の希薄水溶液として用いられる。キトサン
及び／又はキトサン誘導体の使用量は特に限定されない
が９通常は、０．１〜ｌｏＸの濃度にお いて使用され
る。用いられる酸としては。(1) Published Patent Publication No. 180602/1982 (2) Patent Publication No. 1806/1982 (2) Patent Publication No. 106409/1982 In the present invention, chitosan and/or chitosan derivatives are used as an aqueous solution or a dilute aqueous solution of an organic acid or an inorganic acid. Although the amount of chitosan and/or chitosan derivative used is not particularly limited, 9 it is usually used at a concentration of 0.1 to loX. As for the acid used.
塩酸、硝酸などの無機酸や酢酸、クエン酸、コハク酸。Inorganic acids such as hydrochloric acid and nitric acid, acetic acid, citric acid, and succinic acid.
乳酸、およびリンゴ酸などの有機酸を例示することがで
きる。Examples include organic acids such as lactic acid and malic acid.
また、キトサン及び／又はキトサン誘導体の水溶液ある
いは酸性水溶液は、単独もしくはポビドンヨード（ＰＶ
Ｐヨード）、グルコン酸クロルヘキシジン、次亜塩素酸
ナトリウムなどの既存の殺菌剤と混合して用いることも
可能である。In addition, an aqueous solution or an acidic aqueous solution of chitosan and/or a chitosan derivative may be used alone or with povidone iodine (PV
It can also be used in combination with existing disinfectants such as P-iodine), chlorhexidine gluconate, and sodium hypochlorite.
調整したキトサン及び／又はキトサン誘導体含有製剤は
、スプレーまたは浸漬などの方法によって乳頭にキトサ
ン及び／又はキトサン誘導体の被膜を形成せしめ、乳頭
の病原菌の増殖を抑制し、かつ病原菌の***への侵入を
阻止することにより***炎を予防することができる。The prepared preparation containing chitosan and/or chitosan derivatives forms a coating of chitosan and/or chitosan derivatives on the nipples by spraying or dipping, thereby suppressing the growth of pathogenic bacteria on the nipples and preventing pathogens from entering the breast. Mastitis can be prevented by blocking it.
（実施例） 本発明を実施例によりさらに詳細に説明する。(Example) The present invention will be explained in more detail with reference to Examples.
ただし９本発明の範囲は以下の実施例により何等の制約
を受けるものではない。However, the scope of the present invention is not limited in any way by the following examples.
表１に示す成分を有する実施例１及び実施例２のキトサ
ン含有溶液を調製し、以下の試験を行った。Chitosan-containing solutions of Example 1 and Example 2 having the components shown in Table 1 were prepared, and the following tests were conducted.
（表１）
注）
キトサンは７佐倉チッカリンＣＴＡ−４（商品名）を用
いた。(Table 1) Note) Chitosan used was 7Sakura Chikkarin CTA-4 (trade name).
（試験例１）
実施例１のキトサンの希酢酸溶液を用いて搾乳後の乳頭
浸漬を６力月実施したところ、臨床型の***炎発生頭数
が激減した。結果を下記表２に示す。(Test Example 1) When the teats were soaked for 6 months after milking using the chitosan diluted acetic acid solution of Example 1, the number of cows with clinical mastitis was drastically reduced. The results are shown in Table 2 below.
（表２）
頭数 臨床型***炎発生頭数 割合実施例１５２
５ ９．６％′比較例１５２
１３ ２５．ＯＸ ｂ対照例
５４ １４ ２５．９％５（試
験例２）
実施例２のキトサンのクエン酸水溶液を用いて乾乳直後
に１回礼頭浸漬を行い９分娩後も搾乳後の乳頭浸漬を実
施したところ、実施した乳牛１０頭すべてに臨床型***
炎の発生は認められず２体細胞数はいずれの牛も２０万
以下であった。(Table 2) Number of cows Number of cows with clinical mastitis Proportion example 152
5 9.6%' Comparative Example 152
13 25. OX b control example
54 14 25.9%5 (Test Example 2) Using the chitosan citric acid aqueous solution of Example 2, the bow was dipped once immediately after milk drying, and the teat dipped after milking was also carried out after nine calvings. No clinical mastitis was observed in all 10 dairy cows, and the number of somatic cells was less than 200,000 in all cows.
（発明の効果）
この様に本発明を実施することにより臨床型***炎の発
生率は大幅に減少していることが明らかである。(Effects of the Invention) As described above, it is clear that by implementing the present invention, the incidence of clinical mastitis is significantly reduced.
これは、キトサン及びキトサン誘導体の持つ抗菌性を有
する被膜が病原菌の乳頭への侵入を効果的に防いでいる
ためと考えられる。This is thought to be because the antibacterial coating of chitosan and chitosan derivatives effectively prevents pathogenic bacteria from entering the papilla.
また、実施例２に示すように病原菌に感染しやすい時期
である乾乳直後に本発明を実施することにより。Furthermore, as shown in Example 2, by carrying out the present invention immediately after milk drying, which is a time when it is easy to be infected with pathogenic bacteria.
長時間にわたって抗菌性を有する被膜を維持するため。To maintain an antibacterial coating over a long period of time.
乾乳中の感染を防止出来たと考えられる。It is thought that infection during dry milk could be prevented.
本発明のキトサン及び／又はキトサン誘導体含有薬剤は
、乳牛の乳頭に対し刺激が少ないため、実施例１゜２の
いずれの場合においても、乳頭のひび割れなどの薬品の
乳頭への影響は見られなかった。Since the chitosan and/or chitosan derivative-containing drug of the present invention is less irritating to the teats of dairy cows, no effects of the drug on the teats, such as cracking of the teats, were observed in any of the cases of Examples 1 and 2. Ta.
以上のように本発明は、農家にとって有効な家畜の***
炎の予防手段を提供するものである。As described above, the present invention provides a means for preventing mastitis in livestock that is effective for farmers.
出願人 （４３０）日本曹達株式会社 代理人 （７１２５） 構出 吉美 同 （９６４８） 東海 裕作Applicant (430) Nippon Soda Co., Ltd. Agent (7125) Yoshimi Kaide Same (9648) Yusaku Tokai
Claims (1)
体を含有することを特徴とする家畜の***炎予防剤(1) A preventive agent for mastitis for livestock, characterized by containing chitosan and/or a chitosan derivative as an active ingredient
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2290802A JP2648886B2 (en) | 1990-10-30 | 1990-10-30 | How to Prevent Household Mastitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2290802A JP2648886B2 (en) | 1990-10-30 | 1990-10-30 | How to Prevent Household Mastitis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04169529A true JPH04169529A (en) | 1992-06-17 |
| JP2648886B2 JP2648886B2 (en) | 1997-09-03 |
Family
ID=17760682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2290802A Expired - Lifetime JP2648886B2 (en) | 1990-10-30 | 1990-10-30 | How to Prevent Household Mastitis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2648886B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995020968A1 (en) * | 1992-08-07 | 1995-08-10 | Nippon Soda Co., Ltd. | Prophylatic for domestic animal mastitis |
| WO2002067952A1 (en) * | 2001-02-26 | 2002-09-06 | Medicarb Ab | Prophylactic teat treatment |
| WO2003057233A1 (en) * | 2002-01-10 | 2003-07-17 | Rnl Life Science Ltd. | Composition for preventing or treating mastitis of dairy cattle |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52113954A (en) * | 1976-03-19 | 1977-09-24 | Upjohn Co | Local pharmaceutical agent containing clindamycine |
| JPS5927827A (en) * | 1982-08-10 | 1984-02-14 | Shigeo Suzuki | Anti-infective agent |
| JPS63215630A (en) * | 1987-01-16 | 1988-09-08 | アルブライト・エンド・ウィルソン・リミテッド | Medicinal, veterinary and cosmetic composition |
| JPH02107267A (en) * | 1988-08-18 | 1990-04-19 | Minnesota Mining & Mfg Co <3M> | Composition for illing wound |
-
1990
- 1990-10-30 JP JP2290802A patent/JP2648886B2/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52113954A (en) * | 1976-03-19 | 1977-09-24 | Upjohn Co | Local pharmaceutical agent containing clindamycine |
| JPS5927827A (en) * | 1982-08-10 | 1984-02-14 | Shigeo Suzuki | Anti-infective agent |
| JPS63215630A (en) * | 1987-01-16 | 1988-09-08 | アルブライト・エンド・ウィルソン・リミテッド | Medicinal, veterinary and cosmetic composition |
| JPH02107267A (en) * | 1988-08-18 | 1990-04-19 | Minnesota Mining & Mfg Co <3M> | Composition for illing wound |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995020968A1 (en) * | 1992-08-07 | 1995-08-10 | Nippon Soda Co., Ltd. | Prophylatic for domestic animal mastitis |
| WO2002067952A1 (en) * | 2001-02-26 | 2002-09-06 | Medicarb Ab | Prophylactic teat treatment |
| AU2002233873B2 (en) * | 2001-02-26 | 2004-05-06 | Medicarb Ab | Prophylactic teat treatment |
| WO2003057233A1 (en) * | 2002-01-10 | 2003-07-17 | Rnl Life Science Ltd. | Composition for preventing or treating mastitis of dairy cattle |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2648886B2 (en) | 1997-09-03 |
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