JPH04164226A - Fixing solution for inspection of liquid sample of cells - Google Patents
Fixing solution for inspection of liquid sample of cellsInfo
- Publication number
- JPH04164226A JPH04164226A JP2288588A JP28858890A JPH04164226A JP H04164226 A JPH04164226 A JP H04164226A JP 2288588 A JP2288588 A JP 2288588A JP 28858890 A JP28858890 A JP 28858890A JP H04164226 A JPH04164226 A JP H04164226A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- saccharose
- fixative
- solution
- ethanol solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 12
- 238000007689 inspection Methods 0.000 title description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 18
- 229960004793 sucrose Drugs 0.000 claims abstract description 18
- 235000013681 dietary sucrose Nutrition 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000834 fixative Substances 0.000 claims description 18
- 238000012360 testing method Methods 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 abstract description 14
- 239000011521 glass Substances 0.000 abstract description 8
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 239000006059 cover glass Substances 0.000 abstract description 2
- 238000004043 dyeing Methods 0.000 abstract 3
- 230000003247 decreasing effect Effects 0.000 abstract 2
- 238000004299 exfoliation Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 21
- 238000010186 staining Methods 0.000 description 11
- 238000007796 conventional method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000005228 Pericardial Effusion Diseases 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 210000004912 pericardial fluid Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000005315 stained glass Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、液状検体を対象とする細胞診検査用の固定液
に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a fixative for cytodiagnostic tests on liquid specimens.
細胞診検査用固定液として、通常９５％エタノール溶液
が使用されている。A 95% ethanol solution is usually used as a fixative for cytodiagnostic tests.
液状検体としては、尿、脳を髄液、脱水、腹水、関節液
、心嚢液などがあるが、一般にこれらに含まれる細胞の
絶対数は少ない。液状検体は、それを上記の固定液に混
合した後、遠心器を使用して遠沈し、その沈査物をスラ
イドガラスに塗布し標本とするが、それでも細胞数は極
端に少ない。Liquid specimens include urine, cerebrospinal fluid, dehydration, ascites, joint fluid, and pericardial fluid, but the absolute number of cells contained in these is generally small. A liquid specimen is mixed with the above-mentioned fixative and then spun down using a centrifuge, and the precipitate is applied to a slide glass to prepare a specimen, but the number of cells is still extremely small.
個々の細胞を検鏡して、悪性細胞の有無を判定する細胞
診検査にとって、対象となる細胞数が少ないことは、検
査の精度上好ましくない。For cytodiagnostic tests that examine individual cells under a microscope to determine the presence or absence of malignant cells, a small number of target cells is not desirable in terms of test accuracy.
さらにスライドガラスに塗布後、細胞の染色を行うが、
通常行われるパパニコロー染色で水洗も含めて約２０回
の染色液を通すか、その際にスライドガラスに塗布され
た細胞が剥離し、元々少ない細胞数が更に減少する。染
色中の細胞減少は特に液状検体において著しい。Furthermore, after coating the slide glass, the cells are stained.
During conventional Papanicolaou staining, the cells coated on the slide glass will peel off during approximately 20 passes of staining solution, including washing with water, and the originally small number of cells will further decrease. Cell loss during staining is particularly significant in liquid specimens.
本発明は、上記の点に鑑み、固定液に粘張性を与えるこ
とにより、染色中の剥離による細胞数減少を最小限に防
止しつる固定液を提供することを目的とするものである
。In view of the above, an object of the present invention is to provide a fixative that minimizes the decrease in cell number due to detachment during staining by imparting viscosity to the fixative.
上記の目的を達成するために、本発明は、（１） サ
ッカロースを水で溶解後、直ちにエタノ−ル溶液と混合
してなり、
（２） 上記（１）項記載の液状検体細胞診検査用固
定液において、サッカロース溶液とエタノール溶液とを
５５対４５の割合で混合し、サッカロース最終濃度を０
．５％としてなることを特徴とするものである。In order to achieve the above object, the present invention provides (1) sucrose prepared by dissolving saccharose in water and immediately mixing it with an ethanol solution; In the fixative solution, mix the saccharose solution and the ethanol solution in a ratio of 55:45 to bring the final sucrose concentration to 0.
．． 5%.
固定液には、細胞をスライドガラスに粘着させる程度活
眼性を有する物質であって、染色に使用される染色液が
アルコール溶液が多いところから、アルコールに比較的
溶は難い物質という条件を満たすことが要求される。こ
れらの条件を満たすものとしてサッカロースが適してお
り、これをエタノール溶液に溶解し、液状検体細胞診検
査用固定液として使用する。The fixative must be a substance that has a lively effect to the extent that it makes the cells stick to the glass slide, and because the staining solution used for staining is often an alcohol solution, it must meet the requirements of being a substance that is relatively difficult to dissolve in alcohol. is required. Saccharose is suitable as a substance that satisfies these conditions, and is dissolved in an ethanol solution and used as a fixative for liquid specimen cytodiagnosis tests.
この固定液では、サッカロースの混合により、固定液に
適度な活眼性を与え、細胞をスライドガラスに粘着させ
、しかも染色中は、アルコールに比較的不溶性のために
、細胞がスライドガラスより剥離し難くなる。In this fixative, the mixture of saccharose gives the fixative a suitable viscosity and makes the cells adhere to the glass slide.Furthermore, during staining, the cells are relatively insoluble in alcohol, so they are more difficult to detach than the glass slide. Become.
以下本発明の実施例を詳細に説明する。 Examples of the present invention will be described in detail below.
サッカロース水溶液を作製し、それを直ちにエタノール
溶液と混合し、サッカロース最終濃度を０．５％とする
。液状検体１に対し、本発明固定液を２の割合で混合し
固定する。固定後１５００回転５分間遠沈し、その沈査
をスライドガラス上に均等に塗布し、完全乾燥させる。A sucrose aqueous solution is prepared and immediately mixed with an ethanol solution to give a final sucrose concentration of 0.5%. A fixative solution of the present invention is mixed and fixed in a ratio of 1 part to 2 parts of a liquid specimen. After fixation, centrifuge at 1500 rpm for 5 minutes, spread the precipitate evenly on a slide glass, and dry completely.
その後パパニコロー染色を施し、カバーガラスで封入し
て標本を作製する。The sample is then stained with Papanicolaou stain and sealed with a cover glass to prepare a specimen.
染色途中の細胞の減少率を従来法と比較した。The reduction rate of cells during staining was compared with the conventional method.
完全乾燥米固定検体のパパニコロー染色標本（乾燥によ
り細胞に変形を生じさせるため細胞診検査には適さない
か、染色途中での細胞の減少は極めて少ない。）を対照
として比較検討した結果を示すと次の通りである。The following are the results of a comparative study using a Papanicolaou stained specimen of a completely dried fixed rice specimen (drying causes deformation of the cells, so it is not suitable for cytodiagnostic testing, or the loss of cells during staining is extremely small). It is as follows.
固定液の種類 染色法 細胞数の比完全乾燥
未固定 パパニコロー染色 １００なお、
サッカロースを混合したことによるエタノール溶液の固
定力や、染色に及ぼす影響については、本発明固定液使
用の漂木と従来法による標本とで全く相違はなく、サッ
カロースが固定や染色に悪影響を全く与えないことを示
した。Type of fixative Staining method Cell number ratio Completely dry, unfixed Papanicolaou stain 100
Regarding the fixing power of the ethanol solution mixed with saccharose and the effect on staining, there is no difference at all between driftwood using the fixative of the present invention and specimens prepared using the conventional method. It was shown that there is no such thing.
上記の結果から、従来法では、実に８５〜７１％が染色
終了までに、剥離消失しているのに対し、本発明固定液
を使用すると、減少率は、僅か２９〜１８％に留まり、
約７０〜８０％の細胞が検査対象となることがわかる。From the above results, with the conventional method, 85-71% of the peeling disappeared by the end of staining, whereas when using the fixative of the present invention, the reduction rate remained at only 29-18%.
It can be seen that about 70 to 80% of the cells are subject to inspection.
すなわち染色されたスライドガラス標本上の細胞数は、
従来法に比較して、実に約４倍の細胞数となり、検査対
象となる細胞の著量な増加は精度向上につながる。In other words, the number of cells on a stained glass slide specimen is
Compared to the conventional method, the number of cells is approximately four times that of the conventional method, and the significant increase in the number of cells to be tested leads to improved accuracy.
なお、サッカロースを混合したことによるエタノール溶
液の固定力や、染色に及ぼす影響にっていは、本発明固
定液使用の標本と従来法による標本とで全く相違はなく
、サッカロースが固定や染色に悪影響を全く与えないこ
とを示した。またサッカロースは毒性がなく、無臭で安
全である。There is no difference in the fixing power of the ethanol solution and the effect on staining caused by mixing saccharose between specimens using the fixative of the present invention and specimens prepared using the conventional method. showed that it does not give any. Saccharose is also non-toxic, odorless and safe.
−５〜
本発明は、叙上のように構成したから、液状検体細胞診
検査において、染色中の剥離による細胞数減少を最小限
に防止することができ、病院など医療機関や衛生検査所
での液状検体細胞診検査の精度向上に寄与することがで
きる。-5~ Since the present invention is configured as described above, it is possible to minimize the decrease in the number of cells due to peeling during staining in liquid sample cytology tests, and it can be used in medical institutions such as hospitals and sanitary laboratories. This can contribute to improving the accuracy of liquid sample cytology tests.
特許出願人 栄研器材株式会社Patent applicant: Eiken Kizai Co., Ltd.
Claims (2)
液と混合してなる液状検体細胞診検査用固定液。(1) A liquid fixative for specimen cytology tests, which is prepared by dissolving saccharose in water and immediately mixing it with an ethanol solution.
５の割合で混合し、サッカロース最終濃度を０．５％と
してなる特許請求の範囲第１項記載の液状検体細胞診検
査用固定液。(2) 55:4 of sucrose solution and ethanol solution
5. A liquid fixative for a cytodiagnostic test according to claim 1, wherein the fixative is mixed at a ratio of 5% to 5% to give a final saccharose concentration of 0.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2288588A JPH04164226A (en) | 1990-10-29 | 1990-10-29 | Fixing solution for inspection of liquid sample of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2288588A JPH04164226A (en) | 1990-10-29 | 1990-10-29 | Fixing solution for inspection of liquid sample of cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04164226A true JPH04164226A (en) | 1992-06-09 |
Family
ID=17732205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2288588A Pending JPH04164226A (en) | 1990-10-29 | 1990-10-29 | Fixing solution for inspection of liquid sample of cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04164226A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0784246A2 (en) | 1996-01-10 | 1997-07-16 | Canon Kabushiki Kaisha | Image forming apparatus employing intermediary transfer member |
| JP2011158365A (en) * | 2010-02-01 | 2011-08-18 | Fukuyama Rinsho Kensa Center:Kk | Preparation method of liquefied processing cytologic specimen |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63168562A (en) * | 1986-12-30 | 1988-07-12 | Motohide Takahama | Cell immobilizing/preserving liquid |
-
1990
- 1990-10-29 JP JP2288588A patent/JPH04164226A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63168562A (en) * | 1986-12-30 | 1988-07-12 | Motohide Takahama | Cell immobilizing/preserving liquid |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0784246A2 (en) | 1996-01-10 | 1997-07-16 | Canon Kabushiki Kaisha | Image forming apparatus employing intermediary transfer member |
| US5923939A (en) * | 1996-01-10 | 1999-07-13 | Canon Kabushiki Kaisha | Image forming apparatus employing intermediary transfer member |
| JP2011158365A (en) * | 2010-02-01 | 2011-08-18 | Fukuyama Rinsho Kensa Center:Kk | Preparation method of liquefied processing cytologic specimen |
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