JPH0387128A - Nucleus for pearl culture and its production - Google Patents
Nucleus for pearl culture and its productionInfo
- Publication number
- JPH0387128A JPH0387128A JP1318970A JP31897089A JPH0387128A JP H0387128 A JPH0387128 A JP H0387128A JP 1318970 A JP1318970 A JP 1318970A JP 31897089 A JP31897089 A JP 31897089A JP H0387128 A JPH0387128 A JP H0387128A
- Authority
- JP
- Japan
- Prior art keywords
- nucleus
- gelatin
- collagen
- coating
- pearl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 239000011049 pearl Substances 0.000 claims abstract description 29
- 108010035532 Collagen Proteins 0.000 claims abstract description 24
- 102000008186 Collagen Human genes 0.000 claims abstract description 24
- 108010010803 Gelatin Proteins 0.000 claims abstract description 24
- 229920001436 collagen Polymers 0.000 claims abstract description 24
- 239000008273 gelatin Substances 0.000 claims abstract description 24
- 229920000159 gelatin Polymers 0.000 claims abstract description 24
- 235000019322 gelatine Nutrition 0.000 claims abstract description 24
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 24
- 238000000576 coating method Methods 0.000 claims abstract description 23
- 239000011248 coating agent Substances 0.000 claims abstract description 22
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 17
- 230000003115 biocidal effect Effects 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 7
- 238000004132 cross linking Methods 0.000 claims abstract description 4
- 229940088710 antibiotic agent Drugs 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 8
- 239000000126 substance Substances 0.000 abstract description 3
- 241000237502 Ostreidae Species 0.000 description 13
- 235000020636 oyster Nutrition 0.000 description 11
- 239000011162 core material Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 108010045569 atelocollagen Proteins 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000010984 cultured pearl Substances 0.000 description 6
- 241000237852 Mollusca Species 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000005057 Hexamethylene diisocyanate Substances 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- -1 crow Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007159 enucleation Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 241000243321 Cnidaria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 208000012287 Prolapse Diseases 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- XMEVHPAGJVLHIG-FMZCEJRJSA-N chembl454950 Chemical compound [Cl-].C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H]([NH+](C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O XMEVHPAGJVLHIG-FMZCEJRJSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 239000004579 marble Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 229960004989 tetracycline hydrochloride Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、真珠養殖に於ける母貝に挿入する核及びその
製造方法に関し、該核に改良処理を施すことによって、
従来の真珠養殖用の核を用いて養殖した場合に比較し、
品質の高い真珠を高収率で得るようにした技術に関する
。Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a nucleus to be inserted into a mother oyster in pearl culture and a method for producing the same.
Compared to culturing using conventional pearl culturing nuclei,
Concerning technology for obtaining high-quality pearls at a high yield.
（従来の技術及び解決すべき課題）
養殖真珠は、外套膜の切片と真珠の核となる物質をアコ
ヤ貝、シロチョウ貝、クロチョウ貝、マベ、アワビ、イ
ケチョウ貝、カラス貝、ドブ貝、ヒレイケチョウ貝、カ
ワシンシュ貝等の母貝の中に人為的に挿入して生産され
る。現在、養１１１す真珠の核となる物質として真珠層
と成分がほぼ同じドブ貝等の二枚貝の貝殻が主に用いら
れている。この貝殻を研磨し表面を平滑にした後挿入す
る。核を挿入する挿核手術は主として４月〜７月に行な
われているが、貝の生理状態、挿核手術および海況諸条
件、気象状況等により、脱核、貝の死亡等養殖真珠の収
率及び真珠の品質等が大きく影響される。また、現在様
として主に用いられている二枚貝の貝殻等では商品価値
の高い真珠のできる割合が低く、真珠養殖業者の労の割
合には収率が低いといわれている。(Prior art and problems to be solved) Cultured pearls can be produced by using a section of the mantle and the core material of the pearl in pearl oysters, white oysters, black oysters, mabe, abalone, oyster oysters, crow oysters, brown oysters, and oyster oysters. It is produced by artificially inserting it into the mother mollusc of shellfish, snail shellfish, etc. Currently, the shells of bivalves such as the brown oyster, which have almost the same composition as nacre, are mainly used as the core material for pearls. The shell is polished to make its surface smooth and then inserted. Enucleation surgery to insert the nucleus is mainly performed from April to July, but depending on the physiological state of the oyster, the enucleation surgery, sea conditions, weather conditions, etc., the harvest of cultured pearls may occur, such as prolapse or death of the oyster. The rate and quality of pearls are greatly affected. Furthermore, it is said that the bivalve shells that are mainly used today produce pearls with high commercial value at a low rate, and the yield is low compared to the labor of pearl cultivators.
本発明者等は、商品価値の高い真珠ができる割合を高め
るため、人為的に操作できる核に注目し、研究の結果、
真珠の真珠層を作る真珠袋上皮細胞にとってその形成状
態および核との初期接合が極めて重要であり、核の表面
に形成状態の良い真珠袋上皮細胞を作らしめ、且つ核と
の初期接合を良くする後記の特定の物質の被膜を形成す
ることにより上記の問題点を解決しうることを知見し、
本発明を完成した。In order to increase the rate of production of pearls with high commercial value, the present inventors focused on nuclei that can be artificially manipulated, and as a result of research,
For the pearl sac epithelial cells that form the nacreous layer of pearls, their state of formation and initial junction with the nucleus are extremely important. We have discovered that the above problems can be solved by forming a film of the specific substance described below,
The invention has been completed.
（課題を解決するための手段）
本発明は、核表面に、コラーゲン又はゼラチンと抗生物
質の混合物の被膜を厚さ０．００５μｍ以上好ましくは
０．０１〜１００μｍで形成させることにより、形成状
態の良い真珠袋上皮細胞を作らしめ且つ核との初期接合
を良好にするものである。(Means for Solving the Problems) The present invention improves the formation state by forming a coating of a mixture of collagen or gelatin and antibiotics on the nuclear surface with a thickness of 0.005 μm or more, preferably 0.01 to 100 μm. It produces good pearl pouch epithelial cells and improves the initial junction with the nucleus.
上記の改良された養殖真珠の核は、次のようにして製造
される。すなわち、養殖真珠の核をコラーゲン又はゼラ
チンと抗生物質の混合物の０．０１〜１％水溶液でコー
ティング処理し、核表面に厚さ０、００５μｎ】以上の
被膜を形成させる。The improved cultured pearl core described above is manufactured as follows. That is, the core of a cultured pearl is coated with a 0.01 to 1% aqueous solution of a mixture of collagen or gelatin and an antibiotic to form a film with a thickness of 0.005 μm or more on the surface of the core.
本発明で用いるコラーゲン又はゼラチンは均な水溶液を
形成しつるものであることが必要であり、以下に述べる
各種のものが用いられる。The collagen or gelatin used in the present invention must form a homogeneous aqueous solution and be stable, and various types described below can be used.
コラーゲンとしては、牛、ラット、マウス咎の哺乳動物
、更には鳥類、魚類等各種の動物から酸抽出した酸可溶
性コラーゲン、又はこれら動物の不溶性コラーゲンをペ
プシン等の酵素で処理して可溶化したアテロコラーゲン
、又はアルカリ処理により可溶化したコラーゲンである
。抗原性等の刺激の少ないアテロコラーゲンを用いるの
か釘ましい。ゼラチンとしては、一般試薬グレードのも
の又は上記コラーゲンを熱変性して製造したものを使用
する。本発明では、コラーゲン又はゼラチンはそれぞれ
水に溶解する条件で溶解して用い、その濃度は０．０１
〜１％の範囲である。またコラーゲン、ゼラチンは単独
で用いても、混合して用いてもよい。Collagen is acid-soluble collagen extracted from various animals such as cows, rats, and mice, as well as birds and fish, or atelocollagen that is solubilized by treating insoluble collagen from these animals with enzymes such as pepsin. , or collagen solubilized by alkali treatment. It is difficult to use atelocollagen, which has less antigenicity and other irritation. As the gelatin, one of general reagent grade or one produced by thermally denaturing the above-mentioned collagen is used. In the present invention, collagen or gelatin is dissolved in water and used at a concentration of 0.01.
It is in the range of ~1%. Further, collagen and gelatin may be used alone or in combination.
また本発明で用いる抗生物質としては一般に水産業で用
いられている例えばテトラサイクリン、ストレプトマイ
シン、カナマイシンなど水産庁が定める水産用抗生物質
等が挙げられる。。Further, examples of the antibiotic used in the present invention include antibiotics for fisheries generally used in the fisheries industry, such as tetracycline, streptomycin, kanamycin, and the like as specified by the Fisheries Agency. .
本発明においては、核表面にコラーゲン又はゼラチンと
抗生物質との混合溶液をコーティングすることによって
核表面にコラーゲン又はゼラチンと抗生物質とからなる
混合物の被膜を設ける。その際、コラーゲン又はゼラチ
ンに対する抗生物質の割合は、使用する抗生物質の薬効
により異なるが０．１〜２０倍量が好ましい。In the present invention, a coating of a mixture of collagen or gelatin and an antibiotic is provided on the nuclear surface by coating the nuclear surface with a mixed solution of collagen or gelatin and an antibiotic. At this time, the ratio of antibiotic to collagen or gelatin varies depending on the efficacy of the antibiotic used, but is preferably 0.1 to 20 times the amount.
核表面にコラーゲン又はゼラチンと抗生物質とからなる
混合物の被膜を形成することによって、挿入された外套
腹切片は速やかに真珠袋を形成し、有機質層や稜柱層な
ど非真珠層分泌物の分泌を極力抑制し、正常な真珠層を
核に向けて分泌しはじめると共に、抗生物質の抗菌作用
によって脱核率及び母貝の死亡率を低減させることがで
きる。殊に、本発明においては、コラーゲン又はゼラチ
ンと抗生物質とからなる被膜を設けることにより、コラ
ーゲン又はゼラチンが抗生物質の担体（キャリヤー）と
して作用し、抗生物質を徐々に放出する結果、抗生物質
を単独で使用した場合に比して、その抗菌作用を長時間
持続させることができ、そのため脱核率及び母貝の死亡
率をより一層向」ニさせることができる。By forming a coating of a mixture of collagen or gelatin and antibiotics on the nuclear surface, the inserted mantle section quickly forms a pearl sac and inhibits the secretion of non-nacreous secretions such as the organic layer and the trabecular layer. By suppressing this as much as possible, normal nacre begins to be secreted toward the nucleus, and the denucleation rate and mortality rate of the mother oyster can be reduced by the antibacterial action of antibiotics. In particular, in the present invention, by providing a coating consisting of collagen or gelatin and an antibiotic, the collagen or gelatin acts as a carrier for the antibiotic and gradually releases the antibiotic. Compared to when used alone, its antibacterial action can be sustained for a longer period of time, and therefore the denucleation rate and the mortality rate of mother molluscs can be further improved.
コーティング処理は市販のコーティング装置（例えば、
フロイント産業株式会社製の商品名ハイコーター）でお
こなえる。コーティング処理の条件は特に限定されない
が、核の表面にコラーゲン又はゼラチンと抗生物質の混
合物の被膜が０．００５μｍ以上の厚さに形成されるよ
うに行なう。コーティング処理した核は母貝に挿入する
。The coating process is carried out using commercially available coating equipment (e.g.
This can be done with Freund Sangyo Co., Ltd. (trade name: Hi-Coater). The conditions for the coating treatment are not particularly limited, but the coating treatment is performed so that a coating of a mixture of collagen or gelatin and antibiotics is formed on the surface of the nucleus to a thickness of 0.005 μm or more. The coated nucleus is inserted into the mother shell.
また、コーティング処理した核の表面の被膜に架橋処理
を施すことにより、本発明の効果を高めることができる
。架橋処理は、例えば紫外線照射（例、波長２５３．７
ｎｍ）するか、加熱処理するか、０．０１〜１％のゲル
タールアルデヒド溶液（ＰＨ４〜１０）で処理するか、
０．０１〜５％のポリエポキシ化合物溶液（ＰＨ４〜１
０）で処理するか、０．０１〜１％のへキサメチレンジ
イソシアネート溶液で処理する。これら架橋処理した核
は、紫外線照射で架橋処理したものと加熱によるもの以
外は、充分に水洗いし、風乾してから母貝に挿入する。Moreover, the effects of the present invention can be enhanced by subjecting the film on the surface of the coated core to crosslinking treatment. The crosslinking treatment can be carried out, for example, by ultraviolet irradiation (e.g. wavelength 253.7
nm), heat treatment, or treatment with 0.01-1% geltaraldehyde solution (PH4-10),
0.01~5% polyepoxy compound solution (PH4~1
0) or with a 0.01-1% hexamethylene diisocyanate solution. These cross-linked nuclei, except those cross-linked by ultraviolet irradiation and those by heating, are thoroughly washed with water and air-dried before being inserted into the mother shell.
本発１ｉ１Ｊにおいて、コーティング処理する核として
は従来主に用いられていた二枚貝の貝殻のほかに、さん
ご、大理石、セラミック、カラス、合成樹脂、炭酸カル
シウムの成型物等が使用でき、これらを球状、偏平状又
は棒状に加工して用いる。In the present invention 1i1J, in addition to bivalve shells, which have traditionally been mainly used, coral, marble, ceramic, crow, synthetic resin, and molded calcium carbonate can be used as the core for coating, and these can be shaped into spherical shapes. , processed into a flat or rod shape.
また、本発明は、核を貝殻内面に直接接着剤で貼り付け
るか、あるいは貝殻外面より穿孔して外側から核を母貝
内部に入れるかし、貝の外套膜そのものが真珠層分泌器
官となる半形真珠又はスリクォーター真珠の養殖用の核
にも適用できる。これらの核の形は半球状、球状、ドロ
ップ（液滴）状、ハート型状など種々の形である。核の
材質としては上記の各種のものが用いられる。In addition, the present invention allows the shell to be attached directly to the inner surface of the shell with an adhesive, or by making a hole in the outer surface of the shell and inserting the nucleus from the outside into the mother shell. It can also be applied to nuclei for culturing half-shaped pearls or three-quarter pearls. These nuclei have various shapes such as hemispherical, spherical, droplet, and heart-shaped. As the material of the core, the various materials mentioned above are used.
実施例１
アテロコラーゲンｌｏｇにｌｉの水を加え、更にＩＮに
調整した希塩酸を８ｍＱ加え、アテロコラーゲン水溶液
を作る（アテロコラーゲン濃度０．１％、ｐ＋＋３）。Example 1 Li water is added to atelocollagen log, and 8 mQ of diluted hydrochloric acid adjusted to IN is added to prepare an aqueous atelocollagen solution (atelocollagen concentration 0.1%, p++3).
このアテロコラーゲン水溶液に対しアテロコラーゲンと
等重量になるように、テトラサイクリ−
ンを混合する。この混合溶液をフロイント産業（株）製
のコーティング装置（ハイコーター：１ｌＣＴ−旧Ｎｌ
）を用いて、下記の条件にてコーティングし、風乾した
。核にはドブ貝を直径６ ｍ ｍの球に加二Ｉニしたビ
ーズを用いた。Tetracycline is mixed with the atelocollagen aqueous solution so that the weight is equal to that of the atelocollagen. This mixed solution was coated with a coating device manufactured by Freund Sangyo Co., Ltd. (Hi-coater: 1lCT-former Nl
) under the following conditions and air-dried. Beads made by cutting brown sea mollusk into spheres with a diameter of 6 mm were used as the nucleus.
ビーズの仕込み量 ０．６ｋｇスプレー
空気圧 １ｋｇ／ｃｎ丁スプレー空気
量 ２ＯＱｉ分スプレーガン
ＩＩＭ型ｊ個スプレーガンノズル口径
０，７φＩｌｌ Ｉｌｌスプレーガンエアキャッ
プ １．２φｍｍ液温
１１〜１５℃コーティングパン径 ２
００φｍｍパン回転数 ２Ｏｒ、
ｐ８ｍ。Bead preparation amount: 0.6kg Spray air pressure: 1kg/cn Spray air amount: 2OQi spray gun
IIM type j spray gun nozzle diameter
0.7φIll Ill spray gun air cap 1.2φmm Liquid temperature
11-15℃ Coating pan diameter 2
00φmm Pan rotation speed 2Or,
p8m.
上記の条件で被膜の厚さが２０μｍになるようにコーテ
ィングし、これを母貝に挿入した。１年養殖した結果、
歩留がコントロールの約６５％に対し、８５％と向上し
た。また良質法出現率はコントロールの３０％に対し、
４０％と向上した。A coating was applied under the above conditions to a thickness of 20 μm, and this was inserted into the mother shell. As a result of one year of cultivation,
The yield was improved to 85% compared to about 65% in the control. In addition, the appearance rate of the high-quality method was 30% compared to the control.
It improved by 40%.
実施例２
ゼラチン１０ｇに１１の水を加え加熱してゼラチンの水
溶液をつくる（ゼラチン濃度０．１％、ｐ１］６〜８）
。Example 2 Add 11 water to 10 g of gelatin and heat to create an aqueous gelatin solution (gelatin concentration 0.1%, p1] 6-8)
.
このゼラチン水溶液に、塩酸テトラサイクリンをｌｏｇ
加え、実施例１と同一装置、条件でコーティングした。Add log of tetracycline hydrochloride to this aqueous gelatin solution.
In addition, coating was performed using the same equipment and conditions as in Example 1.
所要時間２９０分、使用液量３１０ｍＱであった。この
ビーズ０．６ｋｇを０．１％へキサメチレンジイソシア
ネートＭｅＯＨ溶液２Ｑ中に２０分間浸漬する。その後
ＭｅＯＨ溶液で洗浄し過剰のへキサメチレンジイソシア
ネートを除き、風乾する。この核を母貝に挿入した。１
年養殖した結果、歩留がコントロールの約６５％に対し
、８５χと向上した。また良質法出現率はコントロール
の３０％に対し、４０％と向上した。The time required was 290 minutes, and the amount of liquid used was 310 mQ. 0.6 kg of the beads are immersed in 2Q 0.1% hexamethylene diisocyanate MeOH solution for 20 minutes. Thereafter, excess hexamethylene diisocyanate is removed by washing with MeOH solution and air-dried. This nucleus was inserted into the mother shell. 1
As a result of culturing for 20 years, the yield improved to 85% compared to about 65% of the control. In addition, the appearance rate of high-quality methods was improved to 40% compared to 30% in the control.
（発明の効果）
本発明では、養殖真珠を生産する母貝に挿入する核の表
面に、コラーゲン又はゼラチンと抗生物質との混合物の
被膜を設けであるため、核を母貝に挿核手術で挿入して
も母貝の器官の損傷が最小限に押えられ、且つ損傷がら
の回復も早いため脱核率、母貝の死亡率が低下する。又
形成状態の良い真珠袋上皮細胞が作られ文様との初期接
合が良いため、良質の商品価値の高い養殖真珠を生産す
ることができる。またコラーゲン又はゼラチンは抗生物
質を徐放するので抗生物質の抗菌作用が長期間持続する
。したがって、本発明によれば、良質の真珠を高収率で
生産できる。(Effects of the Invention) In the present invention, the surface of the nucleus to be inserted into the mother oyster for producing cultured pearls is coated with a mixture of collagen or gelatin and antibiotics, so the nucleus can be inserted into the mother oyster through surgery. Even when inserted, damage to the organs of the mother mollusk is kept to a minimum, and the damaged body recovers quickly, so the denucleation rate and the mortality rate of the mother mollusk are reduced. In addition, pearl sac epithelial cells with good formation are produced and the initial bonding with the pattern is good, making it possible to produce cultured pearls of high quality and high commercial value. In addition, since collagen or gelatin releases antibiotics in a sustained manner, the antibacterial action of the antibiotics lasts for a long period of time. Therefore, according to the present invention, high quality pearls can be produced at a high yield.
また、コラーゲン又はゼラチンと抗生物質の混合物は、
水溶液で用いるのでコーティング処理が容易であり、文
物−に被膜を形成できる。Also, a mixture of collagen or gelatin and antibiotics
Since it is used as an aqueous solution, it is easy to coat, and a film can be formed on cultural relics.
Claims (1)
らなる混合物の被膜を、厚さ０．００５μｍ以上で有す
ることを特徴とする真珠養殖用の核。 ２、核が半形真珠又はスリークオーター真珠の養殖用の
核である請求項第１項記載の真珠養殖用の核。 ３、真珠養殖用の核表面を、コラーゲン又はゼラチンと
抗生物質とからなる混合物の０．０１〜０％水溶液でコ
ーティング処理し、核表面に厚さ０．００５μｍ以上の
被膜を形成させたことを特徴とする真珠養殖用の核の製
造方法。 ４、表面に形成された被覆に、架橋処理を施す請求項第
３項記載の真珠養殖用の核の製造方法。[Claims] 1. A nucleus for culturing pearls, which has a coating of a mixture of collagen or gelatin and an antibiotic on the surface of the nucleus, with a thickness of 0.005 μm or more. 2. The nucleus for culturing pearls according to claim 1, wherein the nucleus is a nucleus for culturing half-shaped pearls or three-quarter pearls. 3. Coating the surface of the nucleus for pearl culture with a 0.01-0% aqueous solution of a mixture of collagen or gelatin and antibiotics to form a film with a thickness of 0.005 μm or more on the surface of the nucleus. Features: A method for producing nuclei for pearl culture. 4. The method for producing a nucleus for culturing pearls according to claim 3, wherein the coating formed on the surface is subjected to a crosslinking treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1318970A JPH0387128A (en) | 1989-12-11 | 1989-12-11 | Nucleus for pearl culture and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1318970A JPH0387128A (en) | 1989-12-11 | 1989-12-11 | Nucleus for pearl culture and its production |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1020746A Division JP2724379B2 (en) | 1989-02-01 | 1989-02-01 | Core for pearl culture and method for producing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0387128A true JPH0387128A (en) | 1991-04-11 |
Family
ID=18105026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1318970A Pending JPH0387128A (en) | 1989-12-11 | 1989-12-11 | Nucleus for pearl culture and its production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0387128A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1032260A1 (en) * | 1997-11-17 | 2000-09-06 | Langdon, Susan Jane | Nucleus and method for producing half or mabe pearls |
| FR2808967A1 (en) * | 2000-05-12 | 2001-11-23 | Koken Kk | Cores for cultured pearl production are coated with a water-soluble polymer which is then partially dissolved in sea water |
| JP2004321748A (en) * | 2003-04-25 | 2004-11-18 | Kowa Co Ltd | Washing brush |
| FR2964014A1 (en) * | 2010-08-31 | 2012-03-02 | Ifremer | NUCLEUS COATED WITH A FILMOGENEOUS COATING WITH ANTIBACTERIAL AND CICATRISANT PROPERTIES AND METHOD OF OBTAINING THE SAME |
| CN102731021A (en) * | 2012-07-06 | 2012-10-17 | 广东海洋大学 | Artificial pearl nucleus material and preparation method thereof |
| CN103947592A (en) * | 2014-05-04 | 2014-07-30 | 千足珍珠集团股份有限公司 | Treating fluid used for cultivation of nucleus pearls |
| EP3864959A4 (en) * | 2018-10-12 | 2021-12-22 | FUJIFILM Corporation | Material for pearl culturing, nucleus-inserting method, and composition of material for pearl culturing |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02174621A (en) * | 1988-12-27 | 1990-07-06 | Mikimoto:Kk | Pearl nucleus |
-
1989
- 1989-12-11 JP JP1318970A patent/JPH0387128A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02174621A (en) * | 1988-12-27 | 1990-07-06 | Mikimoto:Kk | Pearl nucleus |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1032260A1 (en) * | 1997-11-17 | 2000-09-06 | Langdon, Susan Jane | Nucleus and method for producing half or mabe pearls |
| EP1032260A4 (en) * | 1997-11-17 | 2004-09-15 | Langdon Susan Jane | Nucleus and method for producing half or mabe pearls |
| FR2808967A1 (en) * | 2000-05-12 | 2001-11-23 | Koken Kk | Cores for cultured pearl production are coated with a water-soluble polymer which is then partially dissolved in sea water |
| JP2004321748A (en) * | 2003-04-25 | 2004-11-18 | Kowa Co Ltd | Washing brush |
| FR2964014A1 (en) * | 2010-08-31 | 2012-03-02 | Ifremer | NUCLEUS COATED WITH A FILMOGENEOUS COATING WITH ANTIBACTERIAL AND CICATRISANT PROPERTIES AND METHOD OF OBTAINING THE SAME |
| WO2012028826A1 (en) * | 2010-08-31 | 2012-03-08 | Ifremer (Institut Français De Recherche Pour L'exploitation De La Mer) | Nucleus coated with a film-forming coating having antibacterial and cicatrizing properties, and method for obtaining same |
| JP2013536681A (en) * | 2010-08-31 | 2013-09-26 | イフレメー（インスティテュー フランセ ドゥ ルシャルシェ プー ロクスプロタシオン ドゥ ラ メー） | Nuclear coating using film-forming coating having antibacterial and scar properties and method for obtaining the same |
| CN102731021A (en) * | 2012-07-06 | 2012-10-17 | 广东海洋大学 | Artificial pearl nucleus material and preparation method thereof |
| CN103947592A (en) * | 2014-05-04 | 2014-07-30 | 千足珍珠集团股份有限公司 | Treating fluid used for cultivation of nucleus pearls |
| EP3864959A4 (en) * | 2018-10-12 | 2021-12-22 | FUJIFILM Corporation | Material for pearl culturing, nucleus-inserting method, and composition of material for pearl culturing |
| US11523592B2 (en) | 2018-10-12 | 2022-12-13 | Fujifilm Corporation | Pearl culture material, nucleus insertion method, and pearl culture material composition |
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