JPH037328B2 - - Google Patents
Info
- Publication number
- JPH037328B2 JPH037328B2 JP60266769A JP26676985A JPH037328B2 JP H037328 B2 JPH037328 B2 JP H037328B2 JP 60266769 A JP60266769 A JP 60266769A JP 26676985 A JP26676985 A JP 26676985A JP H037328 B2 JPH037328 B2 JP H037328B2
- Authority
- JP
- Japan
- Prior art keywords
- artemia
- hydrogen peroxide
- feed
- biological
- rotifer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 36
- 241000238582 Artemia Species 0.000 claims description 22
- 241000251468 Actinopterygii Species 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims 1
- 241000700141 Rotifera Species 0.000 description 10
- 241000233652 Chytridiomycota Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 235000013312 flour Nutrition 0.000 description 4
- 230000012447 hatching Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 241000239250 Copepoda Species 0.000 description 2
- 241000238578 Daphnia Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 241001672739 Artemia salina Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 208000030555 Pygmy Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】
(a) 産業上の利用分野
本発明は、魚類および甲殻類の種苗生産に用い
られる生物餌料（アルテミア、シオミズツボワム
シなど）を効率良く培養することができる方法に
関する。[Detailed Description of the Invention] (a) Field of Industrial Application The present invention relates to a method for efficiently cultivating biological feeds (such as Artemia and Cheryl rotifer) used in the production of seedlings of fish and crustaceans. .
(b) 従来の技術
日本における培養漁業技術は、近年急速な発展
を遂げ、食用魚のかなりの部分が養殖によつて支
えられるようになつた。特に種苗生産の速度を飛
躍的に高めることが可能となつた背景には、各稚
魚用人工飼料及び生物餌料の開発がある。(b) Conventional technology Cultured fisheries technology in Japan has developed rapidly in recent years, and a considerable portion of edible fish has come to be supported by aquaculture. In particular, what has made it possible to dramatically increase the speed of seedling production is the development of artificial feeds and biological feeds for various fry.
現状では、卵からふ化した直後の稚仔魚には、
シオミズツボワムシ、アルテミア、ミジンコなど
の生物餌料が魚の体長に合わせて順次投与されて
いるが、アルテミア（ノープリウス）以後に与え
るべき適当な大きさの生物餌料は見い出されてお
らず、天然海水から採取したプランクトンを利用
している。しかし、天然プランクトン採取は、季
節、気候に左右され易く、安定的な確保が困難で
あるため、アルテミアを養成して従来よりも大き
なアルテミア（体長２〜４mm）を作る試みが行わ
れている。しかしながら、アルテミア養成用飼料
として、これまで小麦粉、大豆粉、パン酵母、米
ヌカ及びこれらを配合した飼料、さらに水溶性ビ
タミンや野菜のエキスなどをブレンドした微粒子
飼料などが作られて利用されているが、いずれも
養成過程におけるアルテミアの死亡率が著しく高
く、体長３〜４mmにまで成長するものは20％程度
であつた。 Currently, young fish immediately after hatching from eggs,
Biological feeds such as Shimizu rotifers, Artemia, and Daphnia are administered sequentially according to the body length of the fish, but no biological feed of an appropriate size to be given after Artemia (nauplii) has been found, and natural seawater It uses plankton collected from However, natural plankton collection is easily affected by the season and climate, and it is difficult to secure a stable supply, so attempts are being made to cultivate Artemia and create larger Artemia (2 to 4 mm in length) than before. However, as feed for Artemia cultivation, flour, soybean flour, baker's yeast, rice bran, feeds containing these ingredients, and microparticle feeds blended with water-soluble vitamins and vegetable extracts have been made and used. However, the mortality rate of Artemia during the cultivation process was extremely high, and only about 20% of them grew to a body length of 3 to 4 mm.
(c) 発明が解決しようとする問題点
本発明の目的は、養成過程における死亡率が低
く、また体長が大きい生物餌料を早く、かつ高密
度で養成することができる。生物飼料の培養法を
提供することにある。(c) Problems to be Solved by the Invention An object of the present invention is to have a low mortality rate during the cultivation process, and to be able to raise large-sized biological feed quickly and at high density. The object of the present invention is to provide a method for cultivating biological feed.
(d) 問題点を解決するための手段
本発明者らは鋭意研究の結果、過酸化水素を培
地に添加することによつてアルテミアの死亡率を
低くし、かつ短期間に体長を大きくすることがで
き、また高密度での養成が可能であることを見い
出した。過酸化水素添加の効果に関しては、過酸
化水素が分解する時の溶存酸素量の上昇、微生物
の殺菌効果及び餌のフロツク化防止といつた培養
環境の向上、さらには卵のふ化促進作用をもつこ
とが原因であると考えられる。(d) Means for solving the problem As a result of intensive research, the present inventors found that by adding hydrogen peroxide to the culture medium, the mortality rate of Artemia can be lowered and the body length can be increased in a short period of time. We discovered that it is possible to train at high density. Regarding the effects of hydrogen peroxide addition, it increases the amount of dissolved oxygen when hydrogen peroxide decomposes, has a sterilizing effect on microorganisms, improves the culture environment by preventing food from becoming flocculent, and has the effect of promoting egg hatching. This is thought to be the cause.
また、アルテミアばかりでなく、上記の方法は
過酸化水素の添加量によつて、シオミズツボワム
シ、ミジンコ、コペポーダなど他の稚魚用生物餌
料の培養にも応用しうることを見出した。 In addition, we have found that the above method can be applied not only to Artemia but also to the cultivation of other biological feeds for young fish, such as the rotifer, Daphnia, and copepoda, depending on the amount of hydrogen peroxide added.
本発明は、かかる知見に基づいて完成されたも
ので、稚魚用生物餌料の培養時に過酸化水素を添
加することを特徴とする稚魚用生物餌料の培養法
である。 The present invention was completed based on this knowledge, and is a method for culturing biological feed for young fish, which is characterized by adding hydrogen peroxide during culturing of the biological feed for young fish.
本発明でいう稚魚用生物餌料とは、シオミズツ
ボワムシ、コガタツボワムシ、カマガタツボワム
シ、アカツボワムシ、カドツボワムシ、ブタペス
トツボワムシなどのワムシ類、アルテミア、コペ
ポーダなどを指す。また、アルテミアとは、
Artemia salinaに代表される海水性動物プラン
クトンでArtemia kausenii、Artemia arietina、
Artemia jekkiiなどが含まれ、一般に養殖生飼
料として利用されているものを指す。 The biological feed for young fish in the present invention refers to rotifers such as Shiomitsu chytrid rotifer, Little chytrid rotifer, Pygmy chytrid rotifer, Red chytrid rotifer, Kado chytrid rotifer, and Butapest chytrid rotifer, Artemia, Copepoda, and the like. Also, what is Artemia?
Saltwater zooplankton represented by Artemia salina, Artemia kausenii, Artemia arietina,
This includes Artemia jekkii, etc., and refers to those commonly used as raw feed for aquaculture.
過酸化水素としては特に制限はなく、通常市販
されている35w／ｖ％の過酸化水素水を用いるの
が便利である。 There are no particular restrictions on the hydrogen peroxide, and it is convenient to use a commercially available 35 w/v % hydrogen peroxide solution.
培地に添加するときの過酸化水素濃度として
は、一般的に１〜3000ppmが好ましい。殺菌効果
の即効性を必要とする場合は、3000ppm以上を用
いることもできるが、その際にはパーオキシダー
ゼまたはカタラーゼとの併用が適当である。パー
オキシダーゼまたはカタラーゼは通常の工業用の
もので充分であり、添加したH2O2を完全に分解
しうる濃度を過酸化水素添加後、５〜10時間後に
加える。 The concentration of hydrogen peroxide when added to the culture medium is generally preferably 1 to 3000 ppm. If an immediate bactericidal effect is required, 3000 ppm or more can be used, but in that case, it is appropriate to use it in combination with peroxidase or catalase. A normal industrial peroxidase or catalase is sufficient, and is added at a concentration sufficient to completely decompose the added H 2 O 2 5 to 10 hours after hydrogen peroxide is added.
なお、過酸化水素はアルカリ、熱、光または外
部の作用要因により分解し、水と酸素になるか
ら、これを利用すれば過酸化水素濃度を制御でき
る。 Note that hydrogen peroxide is decomposed by alkali, heat, light, or external factors to become water and oxygen, and this can be used to control the hydrogen peroxide concentration.
(d) 実施例
実施例 １
アルテミア耐久卵（乾燥品）を26℃の天然海水
中で24時間ふ化させた。ふ化直後のアルテミア幼
生を集め、次のような飼料を用いて過酸化水素を
添加し、またはせずに養成試験を行つた。過酸化
水素添加区には、0.1％過酸化水素濃度となるよ
う市販の35w／ｖ％過酸化水素水を添加した。(d) Examples Example 1 Artemia durable eggs (dried product) were incubated in natural seawater at 26° C. for 24 hours. Artemia larvae were collected immediately after hatching, and rearing tests were conducted using the following feeds with and without hydrogen peroxide. A commercially available 35w/v% hydrogen peroxide solution was added to the hydrogen peroxide addition area to give a hydrogen peroxide concentration of 0.1%.
飼料：
生イースト（粒度を50〜200μにそろえたも
の）
大豆粉（粒度を50〜200μにそろえたもの）
市販の微粒子飼料（ノーサン卵養魚用基礎飼
料「マリンメイト」直径200〜300μ）
ふ化直後のアルテミアの体長は、およそ500μ
で約30個体／mlで養成を開始した。 Feed: Fresh yeast (with a particle size of 50 to 200μ) Soybean flour (with a particle size of 50 to 200μ) Commercially available particulate feed (Norsan egg culture basic feed “Marine Mate” diameter 200 to 300μ) Immediately after hatching The body length of Artemia is approximately 500μ
Cultivation was started with approximately 30 individuals/ml.
なお、飼料の投与量については、いずれも十分
な量（即ち、24時間ごとに投与して、翌日の投与
時には多少の残存飼料が認められる程度）を事前
に検討して与えた。その量は、生イーストは５％
溶液で３ml／／day、市販の微粒子飼料、大豆
粉及び米ヌカは５％固型分の溶液で0.4ml／／
dayである。 The amount of feed administered was determined in advance to ensure a sufficient amount (that is, it was administered every 24 hours, and some residual feed was observed when administered the next day). The amount is 5% for fresh yeast.
3ml//day in solution, commercially available fine particle feed, soybean flour and rice bran in 0.4ml//day in 5% solids solution.
It's day.
以上によるアルテミアの養成結果を図−１及び
図−２に示す。 The results of the Artemia cultivation as described above are shown in Figures 1 and 2.
実施例 ２
実施例１と同様にふ化させたアルテミア幼生を
用いて、培養密度を30個体／ml、60固体／mlとし
て養成した。飼料は生イーストを用い、５％溶液
で30個体／mlの場合３ml／／day、60個体／ml
の場合はこの倍量投与した。過酸化水素添加区
は、0.05％過酸化水素濃度になるよう35w／ｖ％
過酸化水素を加えた。Example 2 Using Artemia larvae hatched in the same manner as in Example 1, cultivation was carried out at a culture density of 30 individuals/ml and 60 individuals/ml. Feed is fresh yeast, 3ml//day for 5% solution of 30 individuals/ml, 60 individuals/ml
In this case, double this amount was administered. Hydrogen peroxide addition area: 35w/v% to make hydrogen peroxide concentration 0.05%
Hydrogen peroxide was added.
結果を図−３に示す。 The results are shown in Figure 3.
(e) 発明の効果
(1) 本発明によれば、従来の養成条件で生残率が
低く、４日目で25％であつたものが、４日目で
50％以上となる。(e) Effects of the invention (1) According to the present invention, the survival rate was low under conventional cultivation conditions, which was 25% on the 4th day, but it decreased on the 4th day.
50% or more.
(2) 本発明によれば、従来の養成条件より体長が
１割以上増加できる。(2) According to the present invention, body length can be increased by 10% or more compared to conventional training conditions.
(3) 従来の養成条件では、密度を高くすると生残
率が早く低下してしまうが、本発明によれば、
高密度でも低密度と全く変わらない培養が可能
となる。(3) Under conventional cultivation conditions, the survival rate decreases quickly when the density is increased, but according to the present invention,
Even at high density, culture is possible that is no different from low density.
図−１及び図−２は、実施例１におけるアルテ
ミアの生残率及び体長の変化を夫々示し、図−３
は実施例２におけるアルテミアの生残率の変化を
示す。
Figures 1 and 2 show the survival rate and body length changes of Artemia in Example 1, respectively, and Figure 3
shows the change in survival rate of Artemia in Example 2.
Claims (1)
加することを特徴とする稚魚用生物餌料の培養
法。 ２ 稚魚用生物餌料がアルテミアである特許請求
の範囲第１項記載の培養法。[Scope of Claims] 1. A method for culturing biological feed for young fish, characterized by adding hydrogen peroxide during culturing of the biological feed for young fish. 2. The culture method according to claim 1, wherein the biological feed for young fish is Artemia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60266769A JPS62126922A (en) | 1985-11-27 | 1985-11-27 | Culture of biological feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60266769A JPS62126922A (en) | 1985-11-27 | 1985-11-27 | Culture of biological feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62126922A JPS62126922A (en) | 1987-06-09 |
| JPH037328B2 true JPH037328B2 (en) | 1991-02-01 |
Family
ID=17435444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60266769A Granted JPS62126922A (en) | 1985-11-27 | 1985-11-27 | Culture of biological feed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62126922A (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62220155A (en) * | 1986-03-19 | 1987-09-28 | Nisshin Oil Mills Ltd:The | High-density rearing of artemia |
| JPH0751028B2 (en) * | 1988-03-10 | 1995-06-05 | 全国漁業協同組合連合会 | Ectoparasite control method for seawater-cultured fish |
| JP2005143329A (en) * | 2003-11-12 | 2005-06-09 | Japan Science & Technology Agency | Method for asepticizing zoo- and phytoplankton and method for culturing rotifer using the same method for asepticization |
| JP4682291B2 (en) * | 2005-02-28 | 2011-05-11 | 国立大学法人北海道大学 | Method for disinfecting rotifer monozygotic eggs |
| JP4859060B2 (en) * | 2007-05-29 | 2012-01-18 | 株式会社片山化学工業研究所 | How to remove Caligus |
| JP7353730B2 (en) * | 2018-02-15 | 2023-10-02 | 株式会社ニッスイ | How to train Artemia |
| WO2023100773A1 (en) | 2021-12-01 | 2023-06-08 | 日本水産株式会社 | Method for rearing artemias, feed for artemia, artemia and artemia population |
-
1985
- 1985-11-27 JP JP60266769A patent/JPS62126922A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62126922A (en) | 1987-06-09 |
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