JPH0367531A - Method for suppressing proliferation of red tide plankton using sea weed - Google Patents
Method for suppressing proliferation of red tide plankton using sea weedInfo
- Publication number
- JPH0367531A JPH0367531A JP1202626A JP20262689A JPH0367531A JP H0367531 A JPH0367531 A JP H0367531A JP 1202626 A JP1202626 A JP 1202626A JP 20262689 A JP20262689 A JP 20262689A JP H0367531 A JPH0367531 A JP H0367531A
- Authority
- JP
- Japan
- Prior art keywords
- red tide
- family
- tide plankton
- ulva
- plankton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001474374 Blennius Species 0.000 title claims abstract description 13
- 230000035755 proliferation Effects 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims description 10
- 239000000284 extract Substances 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims abstract description 3
- 241000196252 Ulva Species 0.000 claims description 9
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 239000000463 material Substances 0.000 claims 1
- 241000989762 Monostroma nitidum Species 0.000 abstract description 2
- 241001261506 Undaria pinnatifida Species 0.000 abstract description 2
- 241000196246 Ulvaceae Species 0.000 abstract 2
- 241000980780 Cladosiphon okamuranus Species 0.000 abstract 1
- 244000184734 Pyrus japonica Species 0.000 abstract 1
- 241000158200 Ulva fasciata Species 0.000 abstract 1
- 241000196259 Ulva pertusa Species 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- 239000000126 substance Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000206758 Heterosigma Species 0.000 description 3
- 101100460719 Mus musculus Noto gene Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 101100187345 Xenopus laevis noto gene Proteins 0.000 description 3
- 239000003957 anion exchange resin Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000218670 Ephedraceae Species 0.000 description 1
- 241000200146 Heterocapsa Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001455273 Tetrapoda Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000000401 methanolic extract Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011403 purification operation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は赤潮プランクトンの増殖抑制方法に関するもの
でさらに詳しくは海藻類特にアオサ科及びモッグ科の海
藻を海中で増殖させるか、あるいは海藻の抽出物、憬た
は破砕物を赤潮発生水域に散布することにより赤潮プラ
ンクトンの細胞を破壊または増殖を阻止する方法に関す
るものである。Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a method for suppressing the proliferation of red tide plankton, and more specifically, the present invention relates to a method for suppressing the proliferation of red tide plankton, and more specifically, a method for inhibiting the proliferation of red tide plankton, and more specifically, a method for inhibiting the proliferation of red tide plankton. The present invention relates to a method of destroying red tide plankton cells or inhibiting their proliferation by spraying a substance, water, or crushed substance into a red tide-occurring area of water.
（従来の技術及び発明の解決しようとする問題点）従来
赤潮を防止するには窒素、リン、などの濃度が大きくな
らないよう海水を汚染しないようにする以外に有効な駆
除方法がなく、−旦発生すると自然消滅を待つしか方法
がなかった。(Problems to be Solved by the Prior Art and the Invention) Conventionally, there is no effective extermination method for preventing red tide other than to prevent the concentration of nitrogen, phosphorus, etc. from becoming too high and to prevent contamination of the seawater. Once it occurred, there was no choice but to wait for it to disappear naturally.
塩素や化学薬品を使用することは毒性が他の魚類、藻類
への影響があって使用出来ないのが現状である。本発明
は海に生える藻類の抽出成分が、赤潮プランクトンの発
育を阻害することを利用して自然界、での赤潮プランク
トンの増殖を抑制、駆除する方法であり、海の他の生物
に対する影響なしに駆除することが出来る。Currently, the use of chlorine and other chemicals cannot be used because of their toxicity, which affects other fish and algae. The present invention is a method for suppressing and exterminating the growth of red tide plankton in the natural world by utilizing extracts from algae that grow in the sea to inhibit the growth of red tide plankton, without affecting other sea creatures. It can be exterminated.
（問題点を解決するための手段）
本発明は海藻が浜に打ち寄せられる４月頃の海水を用−
いて赤潮プランクトンを培養すると育たないことに着目
して、この原因を究明するために約３０種類の海藻のメ
タノール抽出物と熱湯抽出物から陰イオン交換樹脂に吸
着させた物質を分離し、赤潮プランクトンのシャトネラ
トへテロシグマの細胞破壊することが出来る藻を探した
結果、マオサ科のヤプレグサ、アナアオサ、リボンアオ
サ、ヒピミドロ科のヒトエグサ、ワカメ科のワカメの根
、モヅク科の能登モズク、ナガマツモ科のオキナワモズ
？−の７種類が赤潮プランクトンの細胞を破壊すること
がわかった。(Means for solving the problem) The present invention uses seawater around April when seaweed washes up on the beach.
Focusing on the fact that red tide plankton does not grow when cultured in the water, we separated substances adsorbed onto anion exchange resin from methanol extracts and boiling water extracts of about 30 types of seaweed to investigate the cause of this problem. As a result of searching for algae that can destroy the cells of Chatonerat Heterosigma, we found the following: Yapregusa, Ulva, and Ribbon Ulva in the Ephedraceae family, Hitoegusa in the Hypimidaceae family, Wakame root in the Wakameaceae family, Noto Mozuku in the Mozukuraceae family, and Okinawa mozuku in the Nagamaceae family. Seven types of - were found to destroy red tide plankton cells.
自然界の多くの海藻は夏の間はとんど消滅するが、アオ
サ科の海藻は一年中生えているので、アオサが生れるよ
うな新しい石やテトラポットなどを投入した環境をつく
ることも必要である。Most seaweeds in the natural world disappear during the summer, but seaweeds of the Ulva family grow all year round, so it is also possible to create an environment where new stones or tetrapods can be added to allow Ulva to grow. is necessary.
赤潮の発生時に海藻を供給するには冬から春にかけて収
穫、乾燥粉末化しておくか、あるいはメタ−ノールで抽
出した抽出物の粗精製物を準備しておくことも出来た。To supply seaweed during red tide outbreaks, it was possible to harvest it from winter to spring and dry it into powder, or prepare a crude extract extracted with methanol.
この粗精製物はＩＱＰＰＭ濃度で３分以内に赤潮プラン
クトンのギムノデイニウム属、プロロセントラ属、ヘテ
ロカプサ属、ラフイド環のシャツトネラアンチカ、シャ
クトネラマリナ、ヘテロシグマ、のプランクトンの細胞
を破壊することが出来た。This crude product was able to destroy the plankton cells of the red tide plankton of the genus Gymnodenium, Prolocentra, and Heterocapsa, as well as the rhapoid ring species Schactonella antica, Schactonella marina, and Heterosigma, within 3 minutes at IQPPM concentration. .
次に実施例を記す。Next, examples will be described.
実施例 １゜
天日で乾燥した海藻ｌｋｌを５１のメタノールを加えて
２４時間放置して抽出する。抽出したメタノール溶液は
メタノールを留去後の残渣に純水２００肩ｊを加えてと
かしこれにエーテル２００１１４１−加、ｔてクロロフ
ィール、カロチン等のエーテル可溶物は赤潮プランクト
ン破壊物質が含まれていないので除いておく。次に水層
を陽イオン交換樹脂を通して吸着されなかったＰ液を陰
イオン交換樹脂カラムを通した。次に陰イオン交換樹脂
の吸着物をメタノールで溶出し、メタノールを留去後の
残渣が目的の成分である。Example 1° Sun-dried seaweed lkl was extracted by adding 51 methanol and leaving it for 24 hours. After distilling off the methanol, the extracted methanol solution was dissolved by adding 200ml of pure water to the residue, and adding ether 2001141-1 to it. There isn't, so I'll remove it. Next, the aqueous layer was passed through a cation exchange resin, and the unadsorbed P solution was passed through an anion exchange resin column. Next, the adsorbed matter on the anion exchange resin is eluted with methanol, and the residue after distilling off the methanol is the target component.
海藻がアナアオサの場合約５１１の製品が得られた。こ
の物質を分析するためにはイオン交換樹脂での精−製操
作を２回繰返した。製品の赤外吸収スペクトル１１７０
．１０６０．１０３５．７７０．に強い吸収がある。塩
酸及び水酸化ナトリウムでの分解後のペーパークロマト
グラフから脂肪酸グリセロールのスルホキノポースの配
糖体であることがわかった。海藻が能登モズクの場合は
高度な不飽和脂肪酸であることがわかった。When the seaweed was Ulva, approximately 511 products were obtained. In order to analyze this substance, the purification operation using an ion exchange resin was repeated twice. Infrared absorption spectrum of product 1170
．． 1060.1035.770. has strong absorption. Paper chromatography after decomposition with hydrochloric acid and sodium hydroxide revealed that it was a glycoside of fatty acid glycerol sulfoquinopose. It was found that the seaweed Noto Mozuku contains highly unsaturated fatty acids.
アナアオサの抽出製品は２ ＰＰＭの水溶液濃度で１０
分以内に赤潮プランクトンのギムノディニラム属、プロ
ロセントラム属、ヘトロカプサ属、ラフイド環のシャツ
トネラアンチカ、シャットネラマリーナ、ヘテロシグマ
、の細胞を破壊した。Ulva extract products have an aqueous concentration of 2 PPM and 10
Within minutes, the cells of the red tide plankton Gymnodinilum, Prolocentrum, and Hetrocapsa, and the rhapoid rings Shattonella antica, Shattonella marina, and Heterosigma were destroyed.
能登モズクの抽出物についても上記のアオサ科と同様な
操作で分離すると、高度な不飽和脂肪酸であることがわ
かった。この物質は５ ＰＰＭ濃度でｌＯ分以・内にア
ナアオサの抽出物が作用したと同じ赤潮プランクトンの
細胞を破壊した。When the extract of Noto mozuku was separated using the same procedure as for the Ulva family described above, it was found to be highly unsaturated fatty acids. This substance destroyed the same red tide plankton cells that the Ulva Ulva extract did in less than 10 minutes at a concentration of 5 PPM.
Claims (1)
を赤潮発生海域に散布することにより赤潮プランクトン
の増殖を抑制する方法。A method of suppressing the proliferation of red tide plankton by spraying extracts or crushed materials of algae, particularly seaweed of the family Ulva family, Moscus family, in areas where red tide occurs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1202626A JPH0367531A (en) | 1989-08-04 | 1989-08-04 | Method for suppressing proliferation of red tide plankton using sea weed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1202626A JPH0367531A (en) | 1989-08-04 | 1989-08-04 | Method for suppressing proliferation of red tide plankton using sea weed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0367531A true JPH0367531A (en) | 1991-03-22 |
Family
ID=16460473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1202626A Pending JPH0367531A (en) | 1989-08-04 | 1989-08-04 | Method for suppressing proliferation of red tide plankton using sea weed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0367531A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001009457A (en) * | 1999-07-01 | 2001-01-16 | Mitsubishi Heavy Ind Ltd | Device for inactivating or sterilizing harmful microorganism |
| CN103583589A (en) * | 2013-11-27 | 2014-02-19 | 上海海洋大学 | Karenia mikimotoi inhibitor, preparation method and application thereof |
-
1989
- 1989-08-04 JP JP1202626A patent/JPH0367531A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001009457A (en) * | 1999-07-01 | 2001-01-16 | Mitsubishi Heavy Ind Ltd | Device for inactivating or sterilizing harmful microorganism |
| CN103583589A (en) * | 2013-11-27 | 2014-02-19 | 上海海洋大学 | Karenia mikimotoi inhibitor, preparation method and application thereof |
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