JPH0357757B2 - - Google Patents
Info
- Publication number
- JPH0357757B2 JPH0357757B2 JP58111943A JP11194383A JPH0357757B2 JP H0357757 B2 JPH0357757 B2 JP H0357757B2 JP 58111943 A JP58111943 A JP 58111943A JP 11194383 A JP11194383 A JP 11194383A JP H0357757 B2 JPH0357757 B2 JP H0357757B2
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- immobilized
- reaction
- spores
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 34
- 235000014655 lactic acid Nutrition 0.000 claims description 16
- 239000004310 lactic acid Substances 0.000 claims description 16
- 244000005700 microbiome Species 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 235000000346 sugar Nutrition 0.000 claims description 5
- 241000235527 Rhizopus Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 description 16
- 229960000448 lactic acid Drugs 0.000 description 15
- 239000000243 solution Substances 0.000 description 12
- 230000001580 bacterial effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 239000000499 gel Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 240000005384 Rhizopus oryzae Species 0.000 description 4
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 4
- 235000010418 carrageenan Nutrition 0.000 description 4
- 239000000679 carrageenan Substances 0.000 description 4
- 229920001525 carrageenan Polymers 0.000 description 4
- 229940113118 carrageenan Drugs 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 4
- MTPJEFOSTIKRSS-UHFFFAOYSA-N 3-(dimethylamino)propanenitrile Chemical compound CN(C)CCC#N MTPJEFOSTIKRSS-UHFFFAOYSA-N 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000001879 gelation Methods 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000011437 continuous method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 229940117841 methacrylic acid copolymer Drugs 0.000 description 1
- 229920003145 methacrylic acid copolymer Polymers 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000011949 solid catalyst Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
【発明の詳細な説明】
本発明はゲル状担体に固定した乳酸生成能を有
する微生物を糖液と接触反応させ乳酸を製造する
方法にかかり、詳しくは、あらかじめ培養して得
られたリゾプス（Rhizopus）属の乳酸生成能を
有する菌株の菌体または胞子をポリアクリルアミ
ド、カツパー・カラギーナン、アルギン酸などで
ゲル担体に包括固定化し、該固定化微生物を糖液
と接触させる方法である。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing lactic acid by contacting and reacting a microorganism capable of producing lactic acid immobilized on a gel-like carrier with a sugar solution. This is a method in which the cells or spores of a strain of the genus A. ) having the ability to produce lactic acid are entrapping immobilized on a gel carrier using polyacrylamide, Katsupar carrageenan, alginic acid, etc., and the immobilized microorganism is brought into contact with a sugar solution.
この方法では、いわゆる発酵法の如く複雑な副
生物は非常に少なく、精製に容易な状態で工程が
管理できる。 In this method, there are very few complicated by-products as in so-called fermentation methods, and the process can be managed in a state that is easy to purify.
近年、固定化微生物を用い有用な物質を生成さ
せる方法が試みられ、たとえば酵母を使つたエタ
ノールをグルコースから製造する方法は、その代
表例としてあげられる。一方、複雑な生体反応を
用い副生物を生成させずに目的の生成物を得るこ
とはなかなかむずかしいことである。 In recent years, methods for producing useful substances using immobilized microorganisms have been attempted, and a typical example is a method for producing ethanol from glucose using yeast. On the other hand, it is difficult to obtain desired products without producing by-products using complex biological reactions.
リゾプス属の菌体を固定し、この生体反応を利
用し、乳酸を生成させる試みは全くなく本発明を
もつて最初とする。 The present invention is the first in which there has been no attempt to immobilize Rhizopus bacterial cells and utilize this biological reaction to produce lactic acid.
本発明者は乳酸発酵生産にもちいられてたリゾ
プス・オリブ（Rhizopus oryzae）の培養菌体ま
たは胞子をポリアクリルアミド、カツパアー・カ
ラギーナンあるいはアルギン酸カルシウムの各種
ゲルに固定化し、これを球状またはブロツク状に
成型させ、この成型物を固体触媒として通気円筒
反応器をもちい、基質としてグルコースまたはシ
ユクロースを使用し、空気または酸素を通気しな
がら反応させることにより乳酸が得られることを
見出した。本発明は、かかる新規な生物工学的知
見により構成される。 The present inventor immobilized cultured bacterial cells or spores of Rhizopus oryzae, which was used in lactic acid fermentation production, on various gels of polyacrylamide, Katsupaa carrageenan, or calcium alginate, and formed them into spherical or block shapes. They found that lactic acid could be obtained by reacting this molded product as a solid catalyst in a ventilated cylindrical reactor, using glucose or sucrose as a substrate, and aerating air or oxygen. The present invention is constituted by such novel biotechnological findings.
本発明において、乳酸生成能を有する微生物と
してはリゾプス・オリゼAHU6537が適当であ
る。 In the present invention, Rhizopus oryzae AHU6537 is suitable as the microorganism capable of producing lactic acid.
これらの培養条件は一般に知られている乳酸発
酵の場合に、それぞれの菌種に適したものを準ず
れば良い。たとえばリゾプス・オリゼの場合には
15％グルコース、0.05％硝酸アンモン、0.03％燐
酸第１カリウム、0.025％硫酸マグネシウム、２
％炭酸カルシウムを基本とした培地で30℃の通気
条件で深部培養する。これら微生物は集菌、洗浄
して次の包括固定化に供されるが、培養方法は単
なる例であつて何ら本発明を制限するものではな
い。また固体培養により形成される胞子を集め、
これを上記深部培養菌体と同様に本発明に供しう
ることは勿論である。 These culture conditions may be those suitable for each bacterial species in the case of generally known lactic acid fermentation. For example, in the case of Rhizopus oryzae
15% glucose, 0.05% ammonium nitrate, 0.03% potassium phosphate, 0.025% magnesium sulfate, 2
% calcium carbonate-based medium under aeration conditions at 30°C. These microorganisms are collected, washed, and subjected to subsequent entrapping immobilization, but the culture method is merely an example and does not limit the present invention in any way. We also collect spores formed by solid-state culture,
Of course, this can be used in the present invention in the same way as the deep cultured bacterial cells described above.
上記乳酸生成能を有する微生物の包括固定化は
通常公知の微生物菌体の固定化法によつて行なう
ことができるが、とりわけ次に示すゲル化方法を
採用すると効果的である。 The entrapping immobilization of the microorganisms capable of producing lactic acid can be carried out by a commonly known method for immobilizing microbial cells, but it is especially effective to employ the following gelling method.
１） ポリアクリルアミドを固定化剤とする場合
菌体または胞子を生理食塩水に懸濁したものに
アクリルアミドモノマー、Ｎ，N′−メチレン
ビスアクリルアミド、ベータ（β）ジメチルア
ミノプロピオニトリルおよび過硫酸カリウムを
加え室温に放置してゲル化させる。1) When using polyacrylamide as a fixing agent, suspend bacterial cells or spores in physiological saline, add acrylamide monomer, N,N'-methylenebisacrylamide, beta (β) dimethylaminopropionitrile, and potassium persulfate. Add and leave at room temperature to gel.
２） カラギーナンのゲル化方法
上記同様の菌体または胞子を４％カツパー・
カラギーナンと共に加温し、スラリーとしたも
のを注射器により２％塩化カリウム液に滴下、
球状に成型ゲル化する。2) Carrageenan gelling method The same bacterial cells or spores as above were mixed with 4% Katsupaa.
Heat the slurry with carrageenan and drop it into a 2% potassium chloride solution using a syringe.
Form into a spherical gel.
３） アルギン酸のゲル化方法
アクリルアミドゲル化方法で記したと同様に
して得た菌体か胞子の懸濁液に２％になるよう
アルギン酸ナトリウムを加え、30℃に加温、ス
ラリー化したものを注射器により、0.1モル塩
化カルシウム溶液中に滴下凝固させ球状ゲル化
する。3) Alginic acid gelation method Add sodium alginate to 2% to a suspension of bacterial cells or spores obtained in the same manner as described in the acrylamide gelation method, heat to 30°C, and form a slurry. Using a syringe, drop it into a 0.1M calcium chloride solution and solidify to form a spherical gel.
以上に挙げたゲル化法も、単なる例示であり、
ゲル化基剤として、このほか、コラーゲン、セル
ロースサクシネート、カゼインサクシネート、メ
チルアクリレート、メタアクリル酸共重合体など
でも充分本発明の効果は得られる。 The gelation methods mentioned above are also just examples,
In addition to these gelling bases, collagen, cellulose succinate, casein succinate, methyl acrylate, methacrylic acid copolymer, and the like may also be used to fully obtain the effects of the present invention.
本発明の乳酸の製造は、回分式によつても、ま
た連続接触反応によつても実施できる。即ち、固
定化菌体または固定化胞子を、反応に適したPHに
調整した緩衝液に懸濁し、これを円筒型流動層カ
ラムに充填し、これに例えばグルコース、糖蜜な
どの発酵可能な糖を１〜20％濃度添加し、カラム
の下からガラスフイルターなどを通じて通気す
る。通気は余り強いと反応効率が悪化する傾向に
あるので、固定化微生物層が余り乱れないように
努める。反応温度は供試する菌株により多少異な
るが30℃付近が良い。反応液は24時間毎に、新し
い溶液と交換し反応を継続する。 The lactic acid of the present invention can be produced either batchwise or by continuous contact reaction. That is, immobilized bacterial cells or immobilized spores are suspended in a buffer solution adjusted to a pH suitable for the reaction, and this is packed into a cylindrical fluidized bed column, in which fermentable sugars such as glucose and molasses are added. Add 1 to 20% concentration and ventilate from the bottom of the column through a glass filter. If the aeration is too strong, the reaction efficiency tends to deteriorate, so try not to disturb the immobilized microorganism layer too much. The reaction temperature will vary somewhat depending on the bacterial strain being tested, but around 30°C is best. The reaction solution is replaced with fresh solution every 24 hours to continue the reaction.
また連続法による場合、固定化微生物を充填し
たカラムに糖類を含む基質液をペリスタポンプな
どで連続的に送り込み、反応カラムの他方から注
入速度と同じ割合で反応液を流出する。カラムは
必要に応じ温度調節をおこない、反応を至適条件
に保つことにより良い結果を得ることができる。 In the case of a continuous method, a substrate solution containing sugars is continuously fed into a column filled with immobilized microorganisms using a peristaltic pump or the like, and the reaction solution is flowed out from the other side of the reaction column at the same rate as the injection rate. Good results can be obtained by adjusting the temperature of the column as necessary to maintain the reaction at optimal conditions.
以下に実施例をもつて本発明の実施態様を示す
が、固定化微生物の乳酸生成能は反応生成した乳
酸量から求めた。 Embodiments of the present invention are shown below with examples, and the lactic acid production ability of the immobilized microorganism was determined from the amount of lactic acid produced by the reaction.
実施例 １
馬鈴薯寒天に保存してあつたリゾプス・オリゼ
AHU6537の胞子を15％グリコース、0.05％硝酸
アンモン、0.03％燐酸第１カリウム、0.025％硫
酸マグネシウム、２％炭酸カルシウム100mlを入
れた500ml坂口フラスコにて振盪培養し、乳酸生
成活性が高い120〜144時間後、培養液より菌体を
分離し、水にて洗浄する。該洗浄菌体10ｇを0.9
％食塩水32mlに懸濁したものにアクリルアミドモ
ノマー６ｇ、Ｎ，N′−メチレンビスアクリルア
ミド0.32ｇを溶解混合し、さらに５％β−ジメチ
ルアミノプロピオニトリル４mlを加え、更に2.5
％過硫酸カリウム４mlをよく混合し、25℃、15分
間放置し固定化リゾプス菌体を得る。調製された
ゲルを４mm3のブロツクに切断したものを通気撹
拌槽型カラムで通気撹拌させてから、グルコース
５％反応液中で30℃で反応せしめ、24時間毎に５
回反応液を交換した。乳酸生成は24時間の誘導期
のあと各24時間反応でそれぞれ１ｇ／dl、1.2
ｇ／dl、1.4ｇ／dl、1.6ｇ／dlの乳酸溶液を得
た。反応液中には精製に支障をきたす他の有機酸
の副生はみられなかつた。Example 1 Rhizopus oryzae preserved in potato agar
AHU6537 spores were cultured with shaking in a 500ml Sakaguchi flask containing 15% glycose, 0.05% ammonium nitrate, 0.03% potassium phosphate, 0.025% magnesium sulfate, and 2% calcium carbonate, and 120 to 144 with high lactic acid production activity were cultured. After a period of time, the bacterial cells are separated from the culture solution and washed with water. 0.9 for 10g of the washed bacterial cells
Dissolve and mix 6 g of acrylamide monomer and 0.32 g of N,N'-methylenebisacrylamide suspended in 32 ml of 5% brine, add 4 ml of 5% β-dimethylaminopropionitrile, and add 2.5 ml of 5% β-dimethylaminopropionitrile.
Mix 4 ml of % potassium persulfate thoroughly and leave at 25°C for 15 minutes to obtain immobilized Rhizopus cells. The prepared gel was cut into 4 mm 3 blocks, which were aerated and stirred in an aerated stirred tank type column, and then reacted at 30°C in a 5% glucose reaction solution, with 5% incubation every 24 hours.
The reaction solution was exchanged twice. Lactic acid production was 1 g/dl and 1.2 for each 24-hour reaction after a 24-hour induction period, respectively.
g/dl, 1.4 g/dl, and 1.6 g/dl lactic acid solutions were obtained. No other organic acid by-products that would interfere with purification were observed in the reaction solution.
Claims (1)
ゾプス（Rhizopus）属より選ばれた菌株を、空
気または酸素を通気しながら糖液と接触させるこ
とを特徴とする固定化微生物による乳酸の製造
法。1. A method for producing lactic acid using an immobilized microorganism, which comprises bringing a strain selected from the genus Rhizopus immobilized on a gel-like carrier and capable of producing lactic acid into contact with a sugar solution while aerating air or oxygen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11194383A JPS606196A (en) | 1983-06-23 | 1983-06-23 | Production of lactic acid by immobilized microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11194383A JPS606196A (en) | 1983-06-23 | 1983-06-23 | Production of lactic acid by immobilized microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS606196A JPS606196A (en) | 1985-01-12 |
| JPH0357757B2 true JPH0357757B2 (en) | 1991-09-03 |
Family
ID=14574029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11194383A Granted JPS606196A (en) | 1983-06-23 | 1983-06-23 | Production of lactic acid by immobilized microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS606196A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4963486A (en) * | 1989-04-21 | 1990-10-16 | Cornell Research Foundation, Inc. | Direct fermentation of corn to L(+)-lactic acid by Rhizopus oryzae |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57110192A (en) * | 1980-12-26 | 1982-07-08 | Mitsubishi Chem Ind Ltd | Preparation of l-lactic acid |
-
1983
- 1983-06-23 JP JP11194383A patent/JPS606196A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57110192A (en) * | 1980-12-26 | 1982-07-08 | Mitsubishi Chem Ind Ltd | Preparation of l-lactic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS606196A (en) | 1985-01-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4587127A (en) | Process for producing liquid seasoning | |
| EP0054800B1 (en) | Process for the production of immobilized microorganisms | |
| US5939294A (en) | Immobilization of microogranisms on weakly basic anion exchange substance for producing isomaltulose | |
| JPH0416156B2 (en) | ||
| JPS6214274B2 (en) | ||
| CA1215336A (en) | Immobilization of catalytically active microorganisms in agar gel fibers | |
| JPH0357757B2 (en) | ||
| JPS583675B2 (en) | A method for enriching or degrading biochemical compounds contained in an aqueous solution | |
| JPS6043957B2 (en) | Method for producing gluconic acid using immobilized microorganisms | |
| JPS6012037B2 (en) | Production method of itaconic acid using immobilized microorganisms | |
| JPS61209596A (en) | Production of organic acid by immobilized microorganism | |
| JPS585195A (en) | Preparation of citric acid by immobilized microorganism | |
| JPS6258708B2 (en) | ||
| JPS60145095A (en) | Preparation of xylitol by immobilized microorganism | |
| KR20000011105A (en) | Process and carrier for the production of isomaltulose by immobilized micro-organisms | |
| JP2537355B2 (en) | Method for producing sugar alcohol | |
| Chibata | Production of useful chemicals using cells immobilized with polyacrylamide and carrageenan | |
| CN114164238B (en) | Enzymatic synthesis method of L-tyrosine | |
| JPH0634744B2 (en) | Method for producing γ-L-glutamyl-L-α-amino-n-butyrylglycine | |
| JPS60156358A (en) | Production of seasoning solution | |
| JPS5915629B2 (en) | Antibiotic manufacturing method | |
| SU1634715A1 (en) | Method for producing immobilized cells exhibiting glucosoisomerase activity | |
| JPH0759582A (en) | Production of p-hydroxybenzoic acid by immobilized microorganism | |
| JPH0327198B2 (en) | ||
| RU2420581C1 (en) | Method of producing biocatalyst exhibiting cephalosporin acid synthesis activity |