JPH03297378A - Automatic reactor - Google Patents
Automatic reactorInfo
- Publication number
- JPH03297378A JPH03297378A JP9833590A JP9833590A JPH03297378A JP H03297378 A JPH03297378 A JP H03297378A JP 9833590 A JP9833590 A JP 9833590A JP 9833590 A JP9833590 A JP 9833590A JP H03297378 A JPH03297378 A JP H03297378A
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- heat
- heat pipe
- heating
- cooling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000010438 heat treatment Methods 0.000 claims abstract description 44
- 238000001816 cooling Methods 0.000 claims abstract description 38
- 239000011810 insulating material Substances 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims description 106
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 239000000463 material Substances 0.000 claims 1
- 229910052782 aluminium Inorganic materials 0.000 description 11
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 11
- 238000009413 insulation Methods 0.000 description 6
- 239000000110 cooling liquid Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 3
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 101100474383 Escherichia coli (strain K12) rpsO gene Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、温度と反応時間をサイクリックに変化させて
１反応容器に充填したサンプルと酵素の反応を繰り返し
進行・停止させることにより、サンプルの量を増幅させ
る自動反応装置の改良に関し、特に自動反応装置に搭載
される恒温槽の改良に関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention is capable of producing a sample by cyclically changing the temperature and reaction time to repeatedly progress and stop the reaction between a sample and an enzyme filled in one reaction container. This invention relates to an improvement in an automatic reaction device for amplifying the amount of , and in particular, to an improvement in a thermostatic chamber installed in an automatic reaction device.
Ｃ発明の概要ｊ
本発明は、自動反応装置に搭載の恒温槽をフタ部と本体
部の二つに分割し、各部を断熱材で形成し、ヒートパイ
プを反応容器と加熱・冷却源との熱の伝達手段として用
い、ヒートパイプの一端部が反応容器と当接し、他端部
は加熱・冷却源と接する構造とし、恒温槽全体の熱容量
を小さくし、反応容器に充填したサンプルと酵素の温度
を急速（１℃／ ｓ ｅ ｃ以上）に変化させるように
したものである。C Summary of the Invention j The present invention consists of dividing a constant temperature chamber installed in an automatic reaction device into two parts, a lid part and a main body part, forming each part with a heat insulating material, and connecting a heat pipe between the reaction vessel and the heating/cooling source. Used as a means of heat transfer, one end of the heat pipe is in contact with the reaction vessel, and the other end is in contact with the heating/cooling source, reducing the heat capacity of the entire thermostatic chamber and ensuring that the sample and enzyme filled in the reaction vessel are The temperature is changed rapidly (at least 1°C/sec).
［従来の技術］
従来より、温度と反応時間をサイクリックに変化させて
、反応容器に充填したサンプルと酵素の反応を繰り返し
進行・停止させることにより、サンプルの量を増幅させ
る反応をＤＮＡのクローニング・増幅に利用されている
。前記反応を自動的に行なう自動反応装置の恒温槽の構
造を第２図で説明する。第２図で５１はサンプルや酵素
が充填されている反応容器であり、アルミブロック５２
に形成された複数の凹部５２ａ内に収納されている。ア
ルミブロック５２の底面に密着して加熱用シート状ヒー
タ５３を配し、更にその上を断熱材５４で覆われている
。またアルミブロック５２には冷却液用の流路５２ｂが
形成され、前記流路５２ｂは管継手５５により冷凍機（
図示せず）に配管接続されている。反応容器５１を加熱
するときはシート状ヒータ５３に電流を流してアルミブ
ロック５２を加熱する。冷却するときには冷凍機のポン
プにより冷却液をアルミブロック５２内に循環させるこ
とにより行ない、アルミブロック５２を加熱冷却するこ
とにより、アルミブロック５２に形成された複数の凹部
５２ａ内に収納されている反応容器５１を加熱冷却する
。[Prior art] DNA cloning has traditionally been a reaction in which the amount of a sample is amplified by cyclically changing the temperature and reaction time to repeatedly proceed and stop the reaction between a sample filled in a reaction container and an enzyme. -Used for amplification. The structure of a constant temperature bath of an automatic reaction apparatus for automatically carrying out the above reaction will be explained with reference to FIG. In Figure 2, 51 is a reaction container filled with samples and enzymes, and aluminum block 52
It is housed in a plurality of recesses 52a formed in the. A sheet-shaped heater 53 is disposed in close contact with the bottom surface of the aluminum block 52, and is further covered with a heat insulating material 54. Further, a flow path 52b for cooling liquid is formed in the aluminum block 52, and the flow path 52b is connected to the refrigerator (
(not shown). When heating the reaction container 51, a current is passed through the sheet heater 53 to heat the aluminum block 52. Cooling is carried out by circulating a cooling liquid through the aluminum block 52 using the pump of the refrigerator, and by heating and cooling the aluminum block 52, the reactions stored in the plurality of recesses 52a formed in the aluminum block 52 are cooled. The container 51 is heated and cooled.
［発明が解決しようとする課題］
しかし、従来の自動反応装置の恒温槽の構造の場合には
冷却液用の流路５２ｂを形成するためアルミブロック５
２のサイズが大きくなり、熱容量も大きくなる、一方加
熱用シート状ヒータ５３の発熱量に制限があるために、
アルミブロック５２がある程度の温度変化が起きた後で
ないと反応容器内の液の温度が変化を始めないと言うタ
イムラグが大きなものになっていた。また、アルミブロ
ック内に冷却液が残る構造のために、加熱を行なった時
に流路５２ｂの周囲は他の部分に比べて冷却液を加熱す
るのに熱量をとられるため、加熱されるのに時間を要す
る。従ってアルミブロックに温度ムラを生じてしまうと
いう欠点があった。[Problems to be Solved by the Invention] However, in the case of the conventional constant temperature bath structure of an automatic reaction device, the aluminum block 5 is used to form the flow path 52b for the cooling liquid.
2 becomes larger and the heat capacity also becomes larger.On the other hand, since there is a limit to the amount of heat generated by the heating sheet heater 53,
There was a large time lag in that the temperature of the liquid in the reaction vessel did not begin to change until after the aluminum block 52 had undergone a certain degree of temperature change. In addition, due to the structure in which the cooling liquid remains inside the aluminum block, when heating is performed, the area around the flow path 52b takes more heat to heat the cooling liquid than other parts, so it is difficult to heat the area around the flow path 52b. It takes time. Therefore, there is a drawback that temperature unevenness occurs in the aluminum block.
そこで、本発明は従来のこのような欠点を解決するため
に、反応容器収納部を断熱材で形成し、反応容器と加熱
・冷却源間の熱の伝達手段としてヒートパイプを配する
構造にし、恒温槽の熱容量を小さくすることにより、反
応容器内の温度変化を急速（１℃／ ｓ ｅ ｃ以上）
に行なえると共に温度ムラのない恒温槽を得ることを目
的としている。Therefore, in order to solve these conventional drawbacks, the present invention has a structure in which the reaction vessel storage part is formed of a heat insulating material, and a heat pipe is arranged as a heat transfer means between the reaction vessel and the heating/cooling source. By reducing the heat capacity of the thermostatic chamber, the temperature inside the reaction vessel can be rapidly changed (more than 1℃/sec).
The objective is to obtain a constant temperature bath that can be used for a long time and has no temperature unevenness.
［課題を解決するための手段］
上記問題点を解決するためには自動反応装置に搭載の恒
温槽の熱容量を小さくすることにより、加熱・冷却源の
熱が速やかに且つ効率良く反応容器に伝達されることに
なる。従って本発明は反応容器収納部を断熱材で形成し
、反応容器と加熱冷却源間の熱の伝達手段としてヒート
パイプを配する構成とすることにより恒温槽の熱容量を
小さくし、反応容器に充填されたサンプルと酵素の温度
を急速（１℃／ ｓ ｅ ｃ以上）かつ均一に変化させ
るようにしたものである。[Means for solving the problem] In order to solve the above problem, the heat from the heating/cooling source can be quickly and efficiently transferred to the reaction vessel by reducing the heat capacity of the thermostatic chamber installed in the automatic reaction device. will be done. Therefore, the present invention reduces the heat capacity of the thermostatic chamber by forming the reaction container storage part with a heat insulating material and arranging a heat pipe as a heat transfer means between the reaction container and the heating/cooling source. The temperature of the processed sample and the enzyme can be changed rapidly (at least 1°C/sec) and uniformly.
［作用１
上記のように構成された自動反応装置に搭載の恒温槽で
は、加熱・冷却源として熱媒体を流動させてヒートパイ
プの一端部と接触させると、熱は瞬時にヒートパイプの
他端部に伝達される。ヒートパイプの他端部が反応容器
の底部と当接しているので、熱は反応容器を介して反応
容器内のサンプルに伝えられる。この構造の場合の恒温
槽自身の熱容量はヒートパイプの熱容量とほぼ等しい。[Effect 1] In the constant temperature chamber installed in the automatic reaction device configured as above, when a heat medium is made to flow as a heating/cooling source and brought into contact with one end of the heat pipe, heat is instantly transferred to the other end of the heat pipe. The information will be communicated to the department. Since the other end of the heat pipe is in contact with the bottom of the reaction vessel, heat is transferred through the reaction vessel to the sample within the reaction vessel. In this structure, the heat capacity of the constant temperature oven itself is approximately equal to the heat capacity of the heat pipe.
しかしヒートパイプは薄肉のバイブなのでその熱容量は
非常に小さい、また比熱の大きな熱媒体を用いれば熱媒
体が流動中に反応容器等に奪われる熱量の影響による熱
媒体の温度低下は少なくてすむ。従って加熱・冷却に必
要な熱容量は反応容器とサンプルの熱容量と同等程度で
あるので反応容器に充填されたサンプルと酵素の温度を
急速（ｌ”Ｃ／ ｓ ｅ ｃ以上）に且つ均一に変化さ
せることができるようにしたものである。However, since a heat pipe is a thin-walled vibrator, its heat capacity is very small, and if a heat medium with a large specific heat is used, the temperature drop of the heat medium due to the amount of heat taken away by the reaction vessel etc. while the heat medium is flowing can be minimized. Therefore, the heat capacity required for heating and cooling is about the same as that of the reaction vessel and sample, so the temperature of the sample and enzyme filled in the reaction vessel can be changed rapidly (more than 1"C/sec) and uniformly. It has been made possible to do so.
［実施例］ 以下、本発明の実施例１を図面にもとづいて説明する。[Example] Embodiment 1 A first embodiment of the present invention will be described below based on the drawings.
第１図、第３図、第４図において、１はサンプルや酵素
等が充填されている反応容器であり、恒温槽はフタ部２
と本体部３とからなり、４は本体部３を構成する断熱材
料からなる断熱ブロックであり、断熱ブロック４には反
応容器ｌを収納する複数の凹部４ａが形成されている。In Figures 1, 3, and 4, 1 is a reaction container filled with samples, enzymes, etc., and the thermostat is a lid part 2.
and a main body part 3, 4 is a heat insulating block made of a heat insulating material constituting the main body part 3, and the heat insulating block 4 is formed with a plurality of recesses 4a for accommodating the reaction vessels l.
５は反応容器ｌと断熱ブロック４に形成された熱媒体流
動用流路４ｂを流動する熱媒体との間の熱伝達をする下
ヒートパイプであり、凹部４ａと流路４ｂを貫通する穴
に配され、上端部は反応容器１の底部と当接し、下端部
は流路４ｂ内に突設するように配されている。６はフタ
部２を構成する断熱材料からなる断熱プレートであり、
断熱プレート６は複数の反応容器１を覆う位置に配され
、また反応容器ｌのフタ部の逃げが形成されている。７
は反応容器１と断熱プレート６に形成された熱媒体流動
用流路６ｂを流動する熱媒体との間の熱伝達をする上ヒ
ートパイプであり、反応容器１のフタの逃げ部と流路６
ｂを貫通する穴に配され、下端部は反応容器１のフタと
当接し、上端部は流路６ｂ内に突設するように配されて
いる。８はフタ部２に熱媒体を流動させるための管継手
であり、断熱プレート６と螺合されている。９は本体部
３に熱媒体を流動させるための管継手であり、断熱ブロ
ック４と螺合されている。管継手８は制御バルブｌｏａ
のアウト側と断熱チューブ１１で接続され、制御バルブ
ｌｏａのイン側は同じく断熱チューブ１１で加熱ユニッ
ト１２と冷却ユニット１３のアウト側に接続されている
。管継手９は断熱チューブ１１で熱媒体流動用ポンプ１
４のイン側と接続されている。熱媒体流動用ポンプ１４
のアウト側は制御バルブ１０ｂのイン側と接続され、加
熱ユニット１２と冷却ユニット１３のイン側が制御バル
ブｌｏｂのアウト側と接続されている。5 is a lower heat pipe that transfers heat between the reaction vessel l and the heat medium flowing through the heat medium flow channel 4b formed in the heat insulating block 4; The upper end is in contact with the bottom of the reaction vessel 1, and the lower end is arranged to protrude into the flow path 4b. 6 is a heat insulating plate made of a heat insulating material constituting the lid portion 2;
The heat insulating plate 6 is placed in a position to cover the plurality of reaction vessels 1, and a recess for the lid of the reaction vessel 1 is formed. 7
is an upper heat pipe that transfers heat between the reaction vessel 1 and the heat medium flowing through the heat medium flow channel 6b formed in the heat insulating plate 6;
b, its lower end abuts against the lid of the reaction vessel 1, and its upper end projects into the channel 6b. Reference numeral 8 denotes a pipe joint for flowing the heat medium into the lid portion 2, and is screwed onto the heat insulating plate 6. Reference numeral 9 denotes a pipe joint for flowing a heat medium into the main body portion 3, and is screwed together with the heat insulating block 4. Pipe fitting 8 is control valve loa
The in side of the control valve loa is connected to the outside side of the heating unit 12 and the cooling unit 13 through the insulation tube 11. The pipe joint 9 is a heat-insulating tube 11 that connects the pump 1 for heat medium flow.
Connected to the inside side of 4. Heat medium flow pump 14
The outside of the control valve lob is connected to the inside of the control valve 10b, and the inside of the heating unit 12 and the cooling unit 13 are connected to the outside of the control valve lob.
反応容器１を急速加熱するときは、制御バルブ１０ｂが
加熱側に切り換わり、熱媒体流動用ポンプ１４が作動開
始して、熱媒体が加熱ユニット１２に流れ込み、熱媒体
が加熱ユニット１２の中を循環する間に加熱されて、制
御バルブ１０ａを経由して恒温槽に流入する。この時に
フタ部２と本体部３のどちらを先に加熱を開始するかに
より制御バルブ１０ａを切り換える。恒温槽に流入した
熱媒体は流路４ｂと６ｂを流動すると、流路４ｂと６ｂ
内に突設されているヒートパイプ５と７の端部と接して
伝熱を行ない、反応容器１と熱媒体間で熱交換が行なわ
れ、反応容器ｌを急速加熱する。また冷却するときも同
様にして熱媒体が冷却ユニット１３を流動する間に冷却
され、冷却された熱媒体が流路４ｂ、６ｂを流動する時
にヒートパイプ５と７を介して反応容器１と熱交換を行
ない、反応容器ｌを急速冷却する。加熱冷却する時には
反応容器ｌの上部が下部より温度が低いと蒸発した液が
反応容器１の上部に結露し、反応が十分に行なわれない
と言う問題が発生するので、フ夕部２が本体部３よりも
わずかに温度が高くなるように制御バルブ１０ａを制御
する。このように本発明の実施例の恒温槽では恒温槽の
熱容量はヒトパイプの有する熱容量となるが、ヒートパ
イプは銅や銀製の薄肉のパイプ状なので、有する熱容量
は非常に小さい。従って熱媒体の有する熱容量が反応容
器にほぼ１００％伝えられ、反応容器内のサンプルは急
速に加熱・冷却される。本構造の場合では、加熱・冷却
源に熱媒体を用いているので熱容量が大きく、温度変化
の範囲を広く取れる。When rapidly heating the reaction vessel 1, the control valve 10b is switched to the heating side, the heat medium flow pump 14 starts operating, the heat medium flows into the heating unit 12, and the heat medium flows through the heating unit 12. It is heated during circulation and flows into the constant temperature bath via the control valve 10a. At this time, the control valve 10a is switched depending on which of the lid part 2 and the main body part 3 is to be heated first. When the heat medium that has flowed into the thermostatic chamber flows through channels 4b and 6b, it flows through channels 4b and 6b.
Heat is transferred by contacting the ends of the heat pipes 5 and 7 protruding inside, and heat exchange occurs between the reaction vessel 1 and the heat medium, rapidly heating the reaction vessel 1. Also, when cooling, the heat medium is similarly cooled while flowing through the cooling unit 13, and when the cooled heat medium flows through the flow paths 4b and 6b, it is connected to the reaction vessel 1 via the heat pipes 5 and 7. Exchange is performed and reaction vessel 1 is rapidly cooled. During heating and cooling, if the temperature of the upper part of the reaction vessel 1 is lower than that of the lower part, the evaporated liquid will condense on the upper part of the reaction vessel 1, causing a problem that the reaction will not be carried out sufficiently. Control valve 10a is controlled so that the temperature is slightly higher than that in section 3. As described above, in the thermostatic oven of the embodiment of the present invention, the heat capacity of the thermostatic oven is the heat capacity of the human pipe, but since the heat pipe is a thin-walled pipe made of copper or silver, the heat capacity it has is very small. Therefore, almost 100% of the heat capacity of the heat medium is transferred to the reaction vessel, and the sample within the reaction vessel is rapidly heated and cooled. In the case of this structure, since a heat medium is used as the heating/cooling source, the heat capacity is large and the range of temperature change can be widened.
実施例２について第５図で説明する。第５図は反応容器
１の収納部の拡大図であり、２０はサンプルや酵素等の
反応液であり、反応容器ｌに充填されている。２２は恒
温槽のフタ部であり、２６はフタ部２２を構成する断熱
材料からなる断熱プレートであり、断熱プレート２６に
は反応容器１を覆う位置に配されている。７は反応容器
１と断熱プレート２６に形成された熱媒体流動用流路２
６ｂを流動する熱媒体との間の熱伝達をする上ヒートパ
イプであり、断熱プレート２６に形成された貫通穴に配
され、下端部は反応容器１のフタと当接し、上端部は流
路２６ｂ内に突設するように配されている。２９は加熱
用シート状ヒータであり、断熱材からなるプレート３１
と断熱プレート２６の間に積層されている。ヒータ２９
に形成した舌部２９ａがヒートパイプ７の側面に接触し
ている。２４は恒温槽の本体部２３を構成する断熱材料
からなる断熱ブロックであり、断熱ブロック２４には反
応容器１を収納する複数の貫通穴２４ａが形成されてい
る。５は反応容器ｌと断熱材からなるプレート３０に形
成された熱媒体流動用流路３０ａを流動する熱媒体との
間の熱伝達をする下ヒートパイプであり、プレート３ｏ
に形成された貫通穴に配され、上端部は反応容器Ｉの底
部と当接し、下部は流路３０ａ内に突設するように配さ
れている。２８は加熱用シート状ヒータで、断熱ブロッ
ク２４とプレート３０の間に積層されている。ヒータ２
８に形成した舌部２８ａがヒートパイプ５の側面に接触
している。冷却は冷却された熱媒体を流路３０ａ、２６
ｂに流動させて行ない、加熱はシート状ヒータ２８．２
９に通電することで行なう。そして加熱・冷却源と反応
容器１間の熱伝達手段としてヒートパイプ５．７を用い
る。本構造の場合には、加熱源にシート状ヒータを用い
ているので実施例１よりも加熱ユニットやポンプが１台
不用になるので装置が小型化でき、電流の制御で温度制
御が行なえるので温度精度も良くなる。Example 2 will be explained with reference to FIG. FIG. 5 is an enlarged view of the storage section of the reaction container 1, and 20 is a reaction liquid such as a sample or an enzyme, which is filled in the reaction container 1. As shown in FIG. Reference numeral 22 denotes a lid portion of the thermostatic chamber, and 26 denotes a heat insulating plate made of a heat insulating material constituting the lid portion 22. The heat insulating plate 26 is disposed at a position to cover the reaction vessel 1. 7 is a flow path 2 for heat medium flow formed in the reaction vessel 1 and the heat insulating plate 26
6b is an upper heat pipe that transfers heat with the flowing heat medium, and is disposed in a through hole formed in the heat insulating plate 26, the lower end is in contact with the lid of the reaction vessel 1, and the upper end is connected to the flow path. It is arranged so as to protrude within 26b. 29 is a sheet-like heater for heating, and a plate 31 made of a heat insulating material
and a heat insulating plate 26. Heater 29
A tongue portion 29a formed in is in contact with the side surface of the heat pipe 7. Reference numeral 24 denotes a heat insulating block made of a heat insulating material constituting the main body 23 of the thermostatic chamber, and the heat insulating block 24 is formed with a plurality of through holes 24a for accommodating the reaction vessels 1. 5 is a lower heat pipe that transfers heat between the reaction vessel l and the heat medium flowing through the heat medium flow channel 30a formed in the plate 30 made of a heat insulating material;
The upper end portion is in contact with the bottom of the reaction vessel I, and the lower portion is arranged to protrude into the flow path 30a. A sheet-like heater 28 is laminated between the heat insulating block 24 and the plate 30. Heater 2
The tongue portion 28a formed at 8 is in contact with the side surface of the heat pipe 5. For cooling, the cooled heat medium is passed through the channels 30a and 26.
b, and heating is performed using a sheet heater 28.2.
This is done by energizing 9. A heat pipe 5.7 is used as a heat transfer means between the heating/cooling source and the reaction vessel 1. In the case of this structure, since a sheet heater is used as the heating source, one heating unit and pump are not required compared to the first embodiment, so the device can be made smaller, and the temperature can be controlled by controlling the current. Temperature accuracy also improves.
実施例３について第６図で説明する。第６図は反応容器
１の収納部の拡大図であり、６０はサンプルや酵素等の
反応液であり、反応容器ｌに充填されている。６２は恒
温槽のフタ部であり、６６はフタ部６２を構成する断熱
材料からなる断熱プレートであり、断熱プレート６６に
は反応容器１を覆う位置に配され、７は反応容器ｌと加
熱・冷却源間の熱伝達をする上ヒートパイプであり１反
応容器１の断熱プレート６６に形成された貫通穴に配さ
れ、下端部は反応容器１のフタと当接し、上端部は断熱
プレート６６の上面から突設されている。７３はベルチ
ェ素子により冷却される冷却プレートであり、上下に可
動状態で取り付けられている。６４は恒温槽の本体部６
３を構成する断熱材料からなる断熱ブロックであり、断
熱ブロック６４には反応容器１を収納する複数の貫通穴
６４ａが形成されている。５は反応容器１と加熱・冷却
源との間の熱伝達をする下ヒートパイプであり、プレー
ト７０を貫通する穴に配され、上端部は反応容器ｌの底
部と当接し、下部はプレート７０の下面から突設するよ
うに配されている。加熱用シート状ヒータ６８．６９は
実施例２と同じ構造で取り付けられている。冷却はベル
チェ素子を用いて冷却プレート７２．７３を冷却し、冷
却プレート７２．７３は各々がヒートパイプ５．７の端
部と当接する位置まで移動する０反応容器１はヒートパ
イプ５．７を介して冷却プレート７２．７３により冷却
されることになる。加熱はシート状ヒータ６８．６９に
通電すると発熱を行ない、熱量はシート状ヒータ６８．
６９がヒートパイプ５．７と接触しているので、ヒート
パイプ５．７を介して反応容器１に伝えられる。加熱時
には冷却プレート７２．７３はヒートパイプ５．７の端
部と離れる位置に移動する。本構造の場合には、加熱源
にシート状ヒータを用いているので実施例１よりも加熱
・冷却ユニットやポンプが不用になるので装置が小型化
でき、電流の制御で温度制御が行なえるので温度精度も
良くなる。Example 3 will be explained with reference to FIG. FIG. 6 is an enlarged view of the storage section of the reaction container 1, and 60 is a reaction liquid such as a sample or an enzyme, which is filled in the reaction container 1. As shown in FIG. 62 is a lid of the thermostatic chamber; 66 is a heat insulating plate made of a heat insulating material constituting the lid 62; the heat insulating plate 66 is placed in a position to cover the reaction vessel 1; This is an upper heat pipe that transfers heat between the cooling sources, and is disposed in a through hole formed in the heat insulating plate 66 of the reaction vessel 1. The lower end is in contact with the lid of the reaction vessel 1, and the upper end is in contact with the heat insulating plate 66. It protrudes from the top. Reference numeral 73 denotes a cooling plate cooled by a Bertier element, which is attached so as to be movable up and down. 64 is the main body part 6 of the constant temperature bath.
The heat insulating block 64 is made of a heat insulating material and is formed with a plurality of through holes 64a for accommodating the reaction vessels 1. A lower heat pipe 5 transfers heat between the reaction vessel 1 and the heating/cooling source, and is disposed in a hole penetrating the plate 70, the upper end of which is in contact with the bottom of the reaction vessel 1, and the lower part of which is connected to the plate 70. It is arranged so as to protrude from the bottom surface of the. The sheet-like heaters 68 and 69 are installed in the same structure as in the second embodiment. For cooling, the cooling plates 72.73 are cooled using Bertier elements, and the cooling plates 72.73 each move to a position where they abut the ends of the heat pipes 5.7. It will be cooled by cooling plates 72 and 73 via the cooling plates 72 and 73. Heating is performed by applying electricity to the sheet heaters 68 and 69, and the amount of heat is determined by the sheet heaters 68 and 69.
Since the heat pipe 69 is in contact with the heat pipe 5.7, the heat is transmitted to the reaction vessel 1 via the heat pipe 5.7. During heating, the cooling plates 72.73 move away from the ends of the heat pipes 5.7. In the case of this structure, since a sheet-shaped heater is used as the heating source, a heating/cooling unit and a pump are not required compared to Embodiment 1, so the device can be made smaller, and the temperature can be controlled by controlling the current. Temperature accuracy also improves.
次に実施例４について第７図で説明する。第７図は反応
容器ｌの収納部の拡大図であり、実施例１．２．３と同
し構造を有していて、反応容器ｌの側面を覆う形で金属
製のバイブ８２が配され、下端部が熱伝達用ヒートパイ
プ５の上端部と接触している６熱はヒートパイプ５から
バイブ８２を経由して、反応容器１全体に伝えられる。Next, Example 4 will be explained with reference to FIG. FIG. 7 is an enlarged view of the storage section of the reaction vessel 1, which has the same structure as Example 1.2.3, and a metal vibrator 82 is arranged to cover the side surface of the reaction vessel 1. , the lower end of which is in contact with the upper end of the heat transfer heat pipe 5 is transmitted from the heat pipe 5 to the entire reaction vessel 1 via the vibrator 82 .
本構造の場合には、反応容器全体を加熱・冷却できるの
で、反応容器の液量が多い場合でも急速な加熱・冷却が
できる。In the case of this structure, since the entire reaction vessel can be heated and cooled, rapid heating and cooling can be performed even when the amount of liquid in the reaction vessel is large.
以上述べた通り本発明によれば、自動反応装置の恒温槽
の熱容量を小さくすることにより、恒温槽自身を加熱・
冷却する熱容量が小さくてすみ熱媒体の有する熱容量を
サンプルの温度変化に効率良く利用でき、サンプルの温
度を急速に変化させることができると同時に、恒温槽内
を均一な温度にできる。従って、反応容器に充填された
サンプルと酵素の温度を急速（ｌ″Ｃ／ｓｅｃＣ／ｓｅ
ｃ以上に変化させることができる自動反応装置を提供す
ることができる。As described above, according to the present invention, by reducing the heat capacity of the thermostatic chamber of an automatic reaction device, the thermostatic chamber itself can be heated and
Since the heat capacity for cooling is small, the heat capacity of the heating medium can be efficiently used to change the temperature of the sample, and at the same time, the temperature of the sample can be changed rapidly, and at the same time, the temperature inside the thermostatic chamber can be kept uniform. Therefore, the temperature of the sample and enzyme filled in the reaction vessel can be adjusted rapidly (l″C/secC/sec
It is possible to provide an automatic reaction device that can change the temperature by more than c.
第１図は本発明実施例１の正断面図、第２図は従来例の
断面図、第３図は本発明実施例１の恒温槽本体部の平面
図、第４図は本発明実施例１の配管図である。第５図は
本発明の別の実施例２の反応容器収納部の拡大正断面図
、第６図は本発明の別の実施例３の反応容器収納部の拡
大正断面図、第７図は本発明の別の実施例４の反応容器
収納部の拡大正断面図である。
１・・・・・・反応容器
３ ・
４ ・
５ ・ ・
６ ・ ・
７ ・ ・
８、９
１０ ・ ・
１１ ・ ・
ｌ ２ ・ ・
ｌ ３ ・
１４ ・
４
２８、
６ｂ
０ａ
６４ ・
６８．
７２、
フタ部
本体部
断熱ブロック
下ヒートパイプ
断熱プレート
上ヒートパイプ
管継手
制御バルブ
断熱チューブ
加熱ユニット
冷却ユニット
熱媒体流動用ポンプ
断熱ブロック
シート状ヒータ
熱媒体の流路
熱媒体の流路
断熱ブロック
シート状ヒータ
冷却プレート
１８
ｂ
１
第 １ 図
第２図
第
３
閉
＋ｏ（１
３
第
図
第
図
第
闇Fig. 1 is a front sectional view of Embodiment 1 of the present invention, Fig. 2 is a sectional view of the conventional example, Fig. 3 is a plan view of the thermostatic chamber main body of Embodiment 1 of the present invention, and Fig. 4 is an embodiment of the present invention. 1 is a piping diagram. FIG. 5 is an enlarged front cross-sectional view of a reaction container storage section according to another embodiment 2 of the present invention, FIG. 6 is an enlarged front cross-sectional view of a reaction container storage section according to another embodiment 3 of the present invention, and FIG. FIG. 6 is an enlarged front cross-sectional view of a reaction container storage section according to another example 4 of the present invention. 1... Reaction container 3 ・ 4 ・ 5 ・ ・ 6 ・ ・ 7 ・ ・ 8, 9 10 ・ ・ 11 ・ ・ l 2 ・ ・ l 3 ・ 14 ・ 4 28, 6b 0a 64 ・ 68. 72. Lid Main Body Insulation Block Lower Heat Pipe Insulation Plate Upper Heat Pipe Joint Control Valve Insulation Tube Heating Unit Cooling Unit Heat Medium Flow Pump Insulation Block Sheet Heater Heat Medium Flow Path Heat Medium Flow Insulation Block Sheet Heater cooling plate 18 b 1 Fig. 2 Fig. 3 Close + o (1 3 Fig. Fig. Fig. Dark
Claims (5)
し、予め設定されたプログラムに従って加熱・冷却源に
より恒温槽内の温度を変化させて、複数の反応容器各々
に充填したサンプルと酵素の反応を進行、停止させる自
動反応装置において、フタ部と本体部の二つの部分より
構成され、断熱材からなる反応容器収納部を有し、フタ
部と本体部共に反応容器収納数と同数のヒートパイプを
加熱・冷却源と反応容器間の熱伝達手段として用い、複
数の反応容器を上下両方向から同時に加熱・冷却をする
恒温槽を形成したことを特徴とする自動反応装置。(1) It has at least a heating source, a cooling source, and a reaction container storage section, and the temperature in the thermostat is changed by the heating and cooling sources according to a preset program, and the sample and enzyme are filled into each of multiple reaction containers. An automatic reaction device for proceeding and stopping a reaction, which is composed of two parts, a lid part and a main body part, and has a reaction container storage part made of a heat insulating material, and both the lid part and the main body part have the same number of reaction containers as the number of reaction containers stored. An automatic reaction device characterized in that a heat pipe is used as a heat transfer means between a heating/cooling source and a reaction container to form a constant temperature bath that simultaneously heats and cools a plurality of reaction containers from both the upper and lower directions.
数の凹部を形成し、凹部を形成した面と反対側の面に加
熱・冷却用の熱媒体を流動させる流路を形成し、また前
記凹部と流路を接続する貫通穴を設け、貫通穴に一端部
が反応容器と当接し、他端部が熱媒体の流路内に突設す
るようにヒートパイプを配した恒温槽を搭載したことを
特徴とする第１項に記載の自動反応装置。(2) Forming a plurality of recesses for accommodating the reaction vessels in a member for accommodating the reaction vessels, and forming channels for flowing a heat medium for heating and cooling on the surface opposite to the surface on which the recesses are formed; A through hole is provided to connect the recess and the flow path, and a constant temperature chamber is installed in the through hole in which a heat pipe is arranged so that one end is in contact with the reaction vessel and the other end is protruded into the flow path of the heat medium. 2. The automatic reaction device according to item 1, characterized in that:
数の凹部を形成し、凹部を形成した面と反対側の面に冷
却用の熱媒体を流動させる流路を形成し、また前記凹部
と流路を接続する貫通穴を設け、前記貫通穴に一端部が
反応容器と当接し、他端部が熱媒体の流路内に突設する
ようにヒートパイプを配し、また加熱源としてシート状
ヒータを用いて、前記シート状ヒータの一部がヒートパ
イプと接触するように配設したことを特徴とする第１項
に記載の自動反応装置。(3) A plurality of recesses for accommodating the reaction vessels are formed in a member for accommodating the reaction vessels, a flow path for flowing a cooling heat medium is formed on a surface opposite to the surface on which the recesses are formed, and the recesses A heat pipe is provided in the through hole so that one end is in contact with the reaction vessel and the other end is protruded into the heat medium flow path, and a heat pipe is provided as a heat source. 2. The automatic reaction device according to claim 1, wherein a sheet-like heater is used, and a part of the sheet-like heater is arranged so as to be in contact with a heat pipe.
数の凹部を形成し、凹部の底部から反対側の面まで貫通
穴を設け、前記貫通穴に一端部が反応容器と当接し、他
端部が突設するようにヒートパイプを配し、前記突設し
たヒートパイプの一端と圧接したり離れたりできるよう
にペルチェ素子により冷却される冷却プレートが可動状
態に配設され、また加熱源としてシート状ヒータを用い
、前記シート状ヒータの一部がヒートパイプと接触する
ように配設したことを特徴とする第１項に記載の自動反
応装置。(4) Forming a plurality of recesses for accommodating the reaction vessels in a member for accommodating the reaction vessels, providing through holes from the bottom of the recesses to the opposite surface, one end abutting the reaction vessels in the through holes, and the other. A heat pipe is disposed so that its end protrudes, and a cooling plate cooled by a Peltier element is disposed in a movable state so as to be able to come into pressure contact with or separate from one end of the protruding heat pipe, and a heating source 2. The automatic reaction device according to claim 1, wherein a sheet-like heater is used as the heat pipe, and a part of the sheet-like heater is arranged so as to be in contact with a heat pipe.
数の凹部を形成し、前記各凹部の内壁部に熱伝導率の良
好な材料のパイプを配し、前記パイプがヒートパイプの
一部分と接触するようにしたことを特徴とする第１項に
記載の自動反応装置。(5) A plurality of recesses for accommodating the reaction vessels are formed in a member for accommodating the reaction vessels, and a pipe made of a material with good thermal conductivity is arranged on the inner wall of each recess, and the pipe is a part of a heat pipe. 2. The automatic reaction device according to item 1, wherein the automatic reaction device is adapted to be in contact with each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9833590A JPH03297378A (en) | 1990-04-13 | 1990-04-13 | Automatic reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9833590A JPH03297378A (en) | 1990-04-13 | 1990-04-13 | Automatic reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03297378A true JPH03297378A (en) | 1991-12-27 |
Family
ID=14217035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9833590A Pending JPH03297378A (en) | 1990-04-13 | 1990-04-13 | Automatic reactor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03297378A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006075695A (en) * | 2004-09-08 | 2006-03-23 | Shibata Kagaku Kk | Connection adapter |
| JP2007503217A (en) * | 2003-05-23 | 2007-02-22 | バイオ−ラッド ラボラトリーズ，インコーポレイティド | Localized temperature control for spatial arrangement of reaction medium |
| JP2009525847A (en) * | 2006-02-07 | 2009-07-16 | ベックマン コールター， インコーポレイテッド | Method and apparatus for controlling reaction temperature in a biochemical instrument |
| CN105128345A (en) * | 2015-08-10 | 2015-12-09 | 苏州晋翌生物医学仪器有限公司 | Microporous container and manufacture method thereof |
-
1990
- 1990-04-13 JP JP9833590A patent/JPH03297378A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007503217A (en) * | 2003-05-23 | 2007-02-22 | バイオ−ラッド ラボラトリーズ，インコーポレイティド | Localized temperature control for spatial arrangement of reaction medium |
| JP4705035B2 (en) * | 2003-05-23 | 2011-06-22 | バイオ−ラッド ラボラトリーズ，インコーポレイティド | Localized temperature control for spatial arrangement of reaction medium |
| US9623414B2 (en) | 2003-05-23 | 2017-04-18 | Bio-Rad Laboratories, Inc. | Localized temperature control for spatial arrays of reaction media |
| JP2006075695A (en) * | 2004-09-08 | 2006-03-23 | Shibata Kagaku Kk | Connection adapter |
| JP2009525847A (en) * | 2006-02-07 | 2009-07-16 | ベックマン コールター， インコーポレイテッド | Method and apparatus for controlling reaction temperature in a biochemical instrument |
| CN105128345A (en) * | 2015-08-10 | 2015-12-09 | 苏州晋翌生物医学仪器有限公司 | Microporous container and manufacture method thereof |
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