JPH0328196B2 - - Google Patents
Info
- Publication number
- JPH0328196B2 JPH0328196B2 JP18705082A JP18705082A JPH0328196B2 JP H0328196 B2 JPH0328196 B2 JP H0328196B2 JP 18705082 A JP18705082 A JP 18705082A JP 18705082 A JP18705082 A JP 18705082A JP H0328196 B2 JPH0328196 B2 JP H0328196B2
- Authority
- JP
- Japan
- Prior art keywords
- acid
- xmp
- gmp
- culture
- donor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- DCTLYFZHFGENCW-UUOKFMHZSA-N 5'-xanthylic acid Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(NC(=O)NC2=O)=C2N=C1 DCTLYFZHFGENCW-UUOKFMHZSA-N 0.000 claims description 22
- RQFCJASXJCIDSX-UHFFFAOYSA-N 14C-Guanosin-5'-monophosphat Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(O)=O)C(O)C1O RQFCJASXJCIDSX-UHFFFAOYSA-N 0.000 claims description 21
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 claims description 21
- 235000013928 guanylic acid Nutrition 0.000 claims description 21
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 18
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 15
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 15
- 235000002949 phytic acid Nutrition 0.000 claims description 15
- 239000000467 phytic acid Substances 0.000 claims description 15
- 229940068041 phytic acid Drugs 0.000 claims description 15
- 244000005700 microbiome Species 0.000 claims description 11
- 229910021529 ammonia Inorganic materials 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 6
- 239000012736 aqueous medium Substances 0.000 claims description 6
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 241000186146 Brevibacterium Species 0.000 claims description 2
- 241000186216 Corynebacterium Species 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 7
- 235000019341 magnesium sulphate Nutrition 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 239000004202 carbamide Substances 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000013028 medium composition Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- -1 polyoxyethylene stearylamine Polymers 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 241000186145 Corynebacterium ammoniagenes Species 0.000 description 2
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 229940107700 pyruvic acid Drugs 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 241000047703 Nonion Species 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 description 1
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000002280 amphoteric surfactant Substances 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- VAYGXNSJCAHWJZ-UHFFFAOYSA-N dimethyl sulfate Chemical compound COS(=O)(=O)OC VAYGXNSJCAHWJZ-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000003350 kerosene Substances 0.000 description 1
- 229960000448 lactic acid Drugs 0.000 description 1
- 229940094506 lauryl betaine Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- OVGXLJDWSLQDRT-UHFFFAOYSA-L magnesium lactate Chemical compound [Mg+2].CC(O)C([O-])=O.CC(O)C([O-])=O OVGXLJDWSLQDRT-UHFFFAOYSA-L 0.000 description 1
- 239000000626 magnesium lactate Substances 0.000 description 1
- 235000015229 magnesium lactate Nutrition 0.000 description 1
- 229960004658 magnesium lactate Drugs 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 229940099607 manganese chloride Drugs 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- FATBGEAMYMYZAF-KTKRTIGZSA-N oleamide Chemical compound CCCCCCCC\C=C/CCCCCCCC(N)=O FATBGEAMYMYZAF-KTKRTIGZSA-N 0.000 description 1
- 229940113162 oleylamide Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 235000011118 potassium hydroxide Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000002213 purine nucleotide Substances 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000013547 stew Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
本発明は5′−キサンチル酸（以下XMPと記す）
から5′−グアニル酸（以下GMPと記す）を製造
する方法に関する。
GMPは調味料として広く用いられており、
XMPから製造する方法が知られている。
本発明者らは、GMPをより効率的に製造する
方法について研究した結果、水性媒体中でXMP
をGMPに変換する能力を有する微生物をXMPに
作用させてGMPを生成せしめるにあたり、フイ
チン酸単独あるいはフイチン酸とマグネシウムイ
オンを該水性媒体中に添加する事により、著しく
GMPの収率が高くなることを見出した。この発
明はこの知見に基づいて完成されたものである。
以下本発明を詳細に説明する。
XMPをGMPに変換させる反応には、XMPの
他にアンモニア供与体およびATPが必要である。
該ATPは、反応に用いる微生物により生合成さ
れ、反応系に供給される。本発明において、エネ
ルギー供与体とは、GMP生成反応に必要なATP
を、微生物がATP前駆物質（AMP、ADP）か
ら生合成すために必要なエネルギーを供給する化
合物をさす。
本発明において用いる菌株は、ブレビバクテリ
ウム属もしくはコリネバクテリウム属に属する微
生物に紫外線、コバルト60−γ線等の照射を行う
か、またはジメチルサルフエイト、亜硝酸、ニト
ロソグアニジン等の化学処理を行うことにより誘
導された変異株があげられる。たとえばデコイニ
ン耐性株、サイコフラニン耐性株などまたは自然
変異株で、XMPをGMPに変換する能力を有する
ものであればいずれも使用可能である。またこれ
らの性質と同時に他の栄養要求性（例えばアミノ
酸類、ビタミン類、プリン類、ピリミジン類等）
を示す変異株も含まれる。好適な菌株としては例
えばブレビバクテリウム・アンモニアゲネス
ATCC21170およびコリネバクテリウムグルタミ
クムATCC21171があげられる。
微生物の培養に用いる培地組成としては、炭素
源、窒素源、無機塩その他の栄養物などを含み、
使用する微生物の生育を十分に支持するものであ
れば天然培地、人工培地いずれでも使用可能であ
る。すなわち、炭素源としては炭水化物（グルコ
ース、澱粉、糖蜜等）、炭化水素（ノルマルパラ
フイン、ケロセン等）、アルコール（メタノール、
グリセリン、ソルビトール等）、有機酸（ピルビ
ン酸、乳酸、酢酸等）、アミノ酸（グリシン、ア
ラニン、グルタミン酸、アスパラギン酸等）等が
用いられる。
窒素源としては尿素、塩化アンモニウム、液体
アンモニア等、無機塩としては燐酸カリ、硫酸マ
グネシウム、塩化カルシウム、硫酸鉄、塩化マン
ガン、硫酸亜鉛等、その他の栄養物としてはプリ
ンおよびピリミジン系化合物および天然有機物
（肉エキス、酵母エキス、ペプトン、カザミノ酸
等）が使用可能である。栄養要求性を示す菌株を
用いる場合はその要求を満足させる物質を培地中
に存在させる。
微生物の培養条件としては、20〜40℃の温度
で、振盪、通気撹拌等の好気的条件下で、アンモ
ニア、アンモニア水、尿素、苛性カリ、苛性ソー
ダ等を用いてPHを中性付近に調節しながら、１〜
４日間培養を行う。
かくして得られる微生物の培養物は、そのまま
でも水性媒体中においてXMPをGMPに変換する
反応に使用できる。さらに、該培養物を種々処理
して得られる処理物を界面活性剤および／または
有機溶剤を含有する水性媒体中においてXMP、
アンモニア供与体およびエネルギー供与体と接触
反応させてもよい。処理物としては培養物の濃縮
物もしくは乾燥物、培養物を遠心分離機等で処理
して得られる液もしくは菌体、菌体の乾燥物、
アセトン処理物、界面活性剤処理物、音波処理
物、溶菌酵素処理物、固定化菌体あるいは菌体か
らの抽出酵素標品等があげられる。
アンモニア供与体としては、液体アンモニア、
尿素、塩化アンモニウムなどの無機アンモニウム
塩が用いられる。アンモニア供与体は0.1〜20
ｇ／の濃度で使用される。エネルギー供与体と
してはグルコース、フラクトース、シユクロース
加水分解物、糖蜜、澱粉加水分解物など炭水化
物、ピルビン酸、乳酸、酢酸、α−ケトグルタル
酸などの有機酸、グリシン、アラニン、アスパラ
ギン酸、グルタミン酸などのアミノ酸が用いられ
る。エネルギー供与体は５〜150ｇ／の濃度で
使用される。このエネルギー供与体は、微生物を
培養するための培地に添加される炭素源とは区別
される。ただし、培地中に過剰な炭素源が存在
し、該炭素源がGMP生成反応に持ち込まれる場
合は、該炭素源がエネルギー供与体として用いら
れる。
接触反応は水性媒体中であればいずれでも行う
ことができるが、最も好的には、微生物の培養終
了後に、該培養液にXMP等を添加して接触反応
を行わせる方法があげられる。この場合培養終了
時にXMP、アンモニア供与体、糖類、界面活性
剤および／または有機溶剤を加え、さらに必要な
場合はその他の物質を加え、好気的条件下におい
て20〜50℃、好ましくは37℃〜45℃で３〜72時間
反応させることにより目的物を蓄積させることが
できる。その際、PHを６〜８、好ましくは７〜
7.8に調節することが望ましい。
用いるXMPは、高度に精製されたものでも、
またGMPへの変換に支障がなければ粗精製物、
XMPの含有物、もしくはXMP発酵で得られた
XMPを含有する培養液等いずれでも使用可能で
ある。XMPの添加量は、使用菌体標品の変換活
性により異なるが、XMP・Na2・7H2Oとして10
〜100ｇ／が通常用いられる。
用いられる界面活性剤としては、カチオン性界
面活性剤、例えばポリオキシエチレンステアリル
アミン（例えばナイミーンＳ−215、日本油脂K.
K.製）、セチルトリメチアンモニウムブロマイ
ド、セチルピリジウムクロライド等、アニオン性
界面活性剤、例えばナトリウムラウリル硫酸、ナ
トリウムオレイルアミド硫酸、非イオン性界面活
性剤、例えばポリオキシエチレンソルビタンモノ
ステアレート（例えばノニオンST221、日本油脂
K.K.製）等、両性界面活性剤、例えばラウリル
ベタイン（例えばアノンBF、日本油脂K.K.製）
等があげられ、これらは通常0.1〜50ｇ／、好
ましくは１〜20ｇ／の濃度で用いられる。
有機溶剤としては、トルエン、キシレン、脂肪
族アルコール、ベンゼン、酢酸エチルなどが用い
られ、その濃度は0.1〜50ml／、好ましくは１
〜20ml／がよい。
フイチン酸としては、遊離の酸でも、またその
ナトリウム、カリウム等の塩でも、さらにフイチ
ン酸を高濃度に含有する天然物（例えば、フイチ
ン、コーンスチープリカー等）でも、いずれでも
使用できる。その濃度はフイチン酸として１〜50
ｇ／、好ましくは１〜20ｇ／がよい。添加時
期は接触反応の開始時または12時間目までのいず
れの時期でもよく、また微生物の培養物を反応に
用いる場合にはその培養中のいずれの時期でもよ
いが、通常は接触反応開始時に添加する。
マグネシウムイオンは硫酸マグネシウム、塩化
マグネシウム、硝酸マグネシウム、酢酸マグネシ
ウム、乳酸マグネシウム等の無機酸および有機酸
の塩のいずれでも用いられるが、通常は硫酸マグ
シウムが用いられる。マグネシウムイオンの添加
時期はフイチン酸の添加後ならいずれの時期でも
よいが、通常はフイチン酸添加直後から４時間目
までに添加する。添加量は通常１〜５ｇ／であ
り、好ましくは１〜20ｇ／の濃度で用いられ
る。
かくして水性媒体中に生成したGMPを分離・
採取するに際しては、5′−プリンヌクレオチドを
分離・採取する通常の手段、例えばイオン交換樹
脂や活性炭によるクロマトグラフイー等の方法を
用いることができる。
以下に本発明の実施例を示す。
実施例 １
種菌としてブレビバクテリウム・アンモニアゲ
ネスATCC21170株を用い、グルコース７％、ペ
プトン１％、酵母エキス１％、食塩0.3％（PH
7.2）の培地組成で、30℃で20時間培養したもの
を、発酵培地に対して10％（容量）の割合で植菌
した。種培地、発酵培地とも250ml容量の三角フ
ラスコに20mlずつ分注し、殺菌後使用した。発酵
培地は下記の組成のものを用いた。
発酵培地組成：グルコース10％、尿素0.6％、（別
殺菌）、KH2PO41.0％、K2HPO41.0％、
MgSO4・7H2O1.0％、CaCl2・2H2O0.01％、ビ
オチン30μｇ／、酵母エキス0.5％、肉エキス
0.5％。
発酵培地は苛性ソーダを用いてPH7.6に調整し、
殺菌は120℃、10分間加圧条件下で行つた。30で
振盪培養を行い、培養中適宜希アンモニア水でPH
を7.0に調節しながら48時間培養を行つた。
培養終了液10mlを太型試験管に分注し、XMP、
硫安、Na2HPO4、グルコース、ナイミーンＳ−
215をそれぞれ最終濃度で40ｇ／、２ｇ／、
５ｇ／、50ｇ／、12ｇ／となるように添加
した。往復振盪機を用いて、好気的な条件のもと
で、アンモニア水を用いてPHを7.4付近に調節し
つつ、42℃に12時間保つた。フイチン酸およびマ
グネシウムイオンを添加した場合と無添加の場合
のGMP生成量を比較した結果を第１表に示す。
The present invention relates to 5'-xanthylic acid (hereinafter referred to as XMP)
It relates to a method for producing 5'-guanylic acid (hereinafter referred to as GMP) from. GMP is widely used as a seasoning.
A method of manufacturing from XMP is known. As a result of our research on a method to produce GMP more efficiently, we discovered that XMP in an aqueous medium
When a microorganism that has the ability to convert XMP into GMP is used to produce GMP, the addition of phytic acid alone or phytic acid and magnesium ions to the aqueous medium significantly
It was found that the yield of GMP was increased. This invention was completed based on this knowledge. The present invention will be explained in detail below. The reaction that converts XMP to GMP requires an ammonia donor and ATP in addition to XMP.
The ATP is biosynthesized by microorganisms used in the reaction and supplied to the reaction system. In the present invention, the energy donor refers to ATP, which is necessary for the GMP production reaction.
refers to a compound that supplies the energy necessary for microorganisms to biosynthesize from ATP precursors (AMP, ADP). The strain used in the present invention is obtained by irradiating microorganisms belonging to the genus Brevibacterium or Corynebacterium with ultraviolet rays, cobalt-60-γ rays, etc., or chemically treating them with dimethyl sulfate, nitrous acid, nitrosoguanidine, etc. Examples include mutant strains induced by this method. For example, any decoinine-resistant strain, psychofranin-resistant strain, or natural mutant strain that has the ability to convert XMP to GMP can be used. In addition to these properties, other nutritional requirements (e.g. amino acids, vitamins, purines, pyrimidines, etc.)
This also includes mutant strains that exhibit this. Suitable strains include Brevibacterium ammoniagenes, for example.
ATCC21170 and Corynebacterium glutamicum ATCC21171. The medium composition used for culturing microorganisms includes carbon sources, nitrogen sources, inorganic salts and other nutrients,
Either natural or artificial media can be used as long as they sufficiently support the growth of the microorganisms used. In other words, carbon sources include carbohydrates (glucose, starch, molasses, etc.), hydrocarbons (normal paraffin, kerosene, etc.), alcohols (methanol,
Glycerin, sorbitol, etc.), organic acids (pyruvic acid, lactic acid, acetic acid, etc.), amino acids (glycine, alanine, glutamic acid, aspartic acid, etc.), etc. are used. Nitrogen sources include urea, ammonium chloride, liquid ammonia, etc. Inorganic salts include potassium phosphate, magnesium sulfate, calcium chloride, iron sulfate, manganese chloride, zinc sulfate, etc. Other nutrients include purine and pyrimidine compounds and natural organic substances. (meat extract, yeast extract, peptone, casamino acids, etc.) can be used. When using a bacterial strain that exhibits auxotrophy, a substance that satisfies the requirement is present in the medium. The culture conditions for microorganisms include a temperature of 20 to 40°C, under aerobic conditions such as shaking and aeration, and adjusting the pH to around neutrality using ammonia, aqueous ammonia, urea, caustic potash, caustic soda, etc. While, 1~
Cultivate for 4 days. The microbial culture thus obtained can be used as it is in a reaction for converting XMP to GMP in an aqueous medium. Furthermore, the treated products obtained by various treatments of the culture are subjected to XMP,
A catalytic reaction may be performed with an ammonia donor and an energy donor. Processed products include concentrated or dried culture, liquid or bacterial cells obtained by processing the culture with a centrifuge, dried bacterial cells,
Examples include acetone-treated products, surfactant-treated products, sonication products, lytic enzyme-treated products, immobilized bacterial cells, and enzyme preparations extracted from bacterial cells. As the ammonia donor, liquid ammonia,
Inorganic ammonium salts such as urea and ammonium chloride are used. Ammonia donor is 0.1-20
used at a concentration of g/g/g. Energy donors include carbohydrates such as glucose, fructose, sucrose hydrolysates, molasses, and starch hydrolysates, organic acids such as pyruvic acid, lactic acid, acetic acid, and α-ketoglutaric acid, and amino acids such as glycine, alanine, aspartic acid, and glutamic acid. is used. The energy donor is used in a concentration of 5 to 150 g/g/. This energy donor is distinct from the carbon source added to the medium for culturing microorganisms. However, if an excess carbon source is present in the culture medium and the carbon source is carried into the GMP production reaction, the carbon source is used as an energy donor. The contact reaction can be carried out in any aqueous medium, but the most preferable method is to add XMP or the like to the culture solution after culturing the microorganism to carry out the contact reaction. In this case, at the end of the culture, add XMP, ammonia donor, sugars, surfactants and/or organic solvents, and if necessary other substances, under aerobic conditions at 20-50°C, preferably 37°C. The target product can be accumulated by reacting at ~45°C for 3 to 72 hours. At that time, the pH is 6-8, preferably 7-8.
It is desirable to adjust it to 7.8. Even if the XMP used is highly purified,
In addition, if there is no problem with conversion to GMP, crudely purified products,
Contains XMP or obtained by XMP fermentation
Any culture solution containing XMP can be used. The amount of XMP added varies depending on the conversion activity of the bacterial cell preparation used, but it is
~100g/ is usually used. The surfactants used include cationic surfactants such as polyoxyethylene stearylamine (e.g. Nymeen S-215, NOF K.
anionic surfactants such as sodium lauryl sulfate, sodium oleylamide sulfate, nonionic surfactants such as polyoxyethylene sorbitan monostearate (e.g. Nonion ST221, NOF
amphoteric surfactants such as lauryl betaine (e.g. Anon BF, manufactured by NOF KK);
These are usually used at a concentration of 0.1 to 50 g/, preferably 1 to 20 g/. As the organic solvent, toluene, xylene, aliphatic alcohol, benzene, ethyl acetate, etc. are used, and the concentration thereof is 0.1 to 50 ml, preferably 1
~20ml/ is good. As phytic acid, any of the free acid, its salts such as sodium and potassium salts, and natural products containing high concentrations of phytic acid (eg, phytic acid, corn steep liquor, etc.) can be used. Its concentration is 1-50 as phytic acid
g/, preferably 1 to 20 g/. The addition time may be at the start of the contact reaction or up to the 12th hour, and if a culture of microorganisms is used for the reaction, it may be added at any time during the culture, but it is usually added at the start of the contact reaction. do. As the magnesium ion, any salt of an inorganic acid or an organic acid such as magnesium sulfate, magnesium chloride, magnesium nitrate, magnesium acetate, or magnesium lactate can be used, but magnesium sulfate is usually used. Magnesium ions may be added at any time after the addition of phytic acid, but it is usually added by 4 hours after the addition of phytic acid. The amount added is usually 1 to 5 g/, preferably 1 to 20 g/. In this way, the GMP generated in the aqueous medium can be separated and
For collection, conventional means for separating and collecting 5'-purine nucleotides, such as chromatography using ion exchange resins or activated carbon, can be used. Examples of the present invention are shown below. Example 1 Brevibacterium ammoniagenes ATCC21170 strain was used as the inoculum, 7% glucose, 1% peptone, 1% yeast extract, 0.3% salt (PH
The medium composition described in 7.2) was cultured at 30°C for 20 hours and inoculated into the fermentation medium at a rate of 10% (volume). Both the seed medium and the fermentation medium were dispensed into 250 ml Erlenmeyer flasks and used after sterilization. The fermentation medium used had the following composition. Fermentation medium composition: glucose 10%, urea 0.6%, (separate sterilization), KH 2 PO 4 1.0%, K 2 HPO 4 1.0%,
MgSO4・7H2O1.0 %, CaCl2・2H2O0.01 %, biotin 30μg/, yeast extract 0.5%, meat extract
0.5%. The fermentation medium was adjusted to pH 7.6 using caustic soda.
Sterilization was performed under pressure at 120°C for 10 minutes. Perform shaking culture at 30℃, and adjust pH with dilute ammonia water as needed during culture.
Culture was carried out for 48 hours while adjusting the temperature to 7.0. Dispense 10ml of the cultured solution into a large test tube, add XMP,
Ammonium sulfate, Na 2 HPO 4 , glucose, Naimeen S-
215 at final concentrations of 40g/, 2g/, respectively.
They were added in amounts of 5g/, 50g/, and 12g/. Using a reciprocating shaker, the mixture was kept at 42°C for 12 hours under aerobic conditions while adjusting the pH to around 7.4 using aqueous ammonia. Table 1 shows the results of comparing the amount of GMP produced when phytic acid and magnesium ions were added and when they were not added.
【表】
実施例 ２
実施例１と同じ菌株を、発酵培地のグルコース
を18％とした以外は同じ条件で培養し、該培養終
了後５mlを分注した太型試験管にXMP発酵培養
液（XMP・Na2・7H2O70ｇ／含有）５mlとそ
の他の必要成分を実施例１と同様に添加し、実施
例１と同様に反応した結果を第２表に示す。[Table] Example 2 The same strain as in Example 1 was cultured under the same conditions except that the glucose content of the fermentation medium was changed to 18%. After the cultivation was completed, 5 ml of the XMP fermentation culture solution ( 5 ml of XMP・Na 2・7H 2 O (70 g/containing) and other necessary components were added in the same manner as in Example 1, and the reaction was carried out in the same manner as in Example 1. The results are shown in Table 2.
【表】
実施例 ３
実施例１と同じ菌株を用い培養条件、および反
応条件で、フイチン酸およびマグネシウムイオン
を種々の量添加して、生成するGMPの量を調べ
た。結果を第３表に示す。[Table] Example 3 Using the same bacterial strain as in Example 1, various amounts of phytic acid and magnesium ions were added under culture conditions and reaction conditions, and the amount of GMP produced was investigated. The results are shown in Table 3.
【表】
実施例 ４
ナイミーンＳ−215（10ｇ／）およびキシレン
（４ml／）を添加する直前にフイチン酸を10
ｇ／添加し、ナイミーンＳ−215を添加した直
後から５時間目までの各時期に硫酸マグネシウム
（５ｇ／）を添加した以外は実施例３と同様の
条件でGMPの生成量を調べた。第４表にその結
果を示す。[Table] Example 4 Immediately before adding Naimeen S-215 (10 g/) and xylene (4 ml/), 10% of phytic acid was added.
The amount of GMP produced was examined under the same conditions as in Example 3, except that magnesium sulfate (5 g/) was added at each time from immediately after adding Naimeen S-215 to 5 hours. Table 4 shows the results.
【表】
実施例 ５
種菌としてコリネバクテリウム・グルタミクム
ATCC21171を用いた。種培地として実施例１に
示したものを、発酵培地として下記の組成のもの
を、それぞれ用いた。
発酵培地組成：澱粉加水分解液（グルコース換算
15％）、尿素２ｇ／、NH4Cl3ｇ／、
KH2PO410ｇ／、K2HPO410ｇ／、
MgSO4・7H2O10ｇ／、MnCl2・4H2O5mg／
、コーンスチーブリカー10ｇ／、ペプトン
２ｇ／（PH7.6）。
以下実施例１と同じ条件で培養を行い、フイチ
ン酸添加量を10ｇ／、硫酸マグネシウム添加量
を５ｇ／としたほかは実施例１と同じ条件で
GMP・Na2・7H2Oの生成量を調べた結果、26.5
ｇ／生成した。なお、フイチン酸および硫酸マ
グネシウム無添加の場合の生成量は13.2ｇ／で
あつた。[Table] Example 5 Corynebacterium glutamicum as inoculum
ATCC21171 was used. The seed medium shown in Example 1 was used, and the fermentation medium with the following composition was used. Fermentation medium composition: Starch hydrolyzate (converted to glucose)
15%), urea 2g/, NH 4 Cl3g/,
KH 2 PO 4 10g/, K 2 HPO 4 10g/,
MgSO 4・7H 2 O 10g/, MnCl 2・4H 2 O 5mg/
, corn stew liquor 10g/, peptone 2g/(PH7.6). The following culture was carried out under the same conditions as in Example 1, except that the amount of phytic acid added was 10 g/, and the amount of magnesium sulfate was 5 g/.
As a result of investigating the production amount of GMP・Na 2・7H 2 O, it was found that 26.5
g/produced. In addition, the production amount in the case where phytic acid and magnesium sulfate were not added was 13.2 g/.
Claims (1)
ウム属に属し、5′−キサンチル酸、アンモニア供
与体およびエネルギー供与体から5′−グアニル酸
を生成する能力を有する微生物の培養物、菌体ま
たはそれらの処理物と5′−キサンチル酸、アンモ
ニア供与体およびエネルギー供与体とを界面活性
剤および／または有機溶剤を含有する水性媒体中
においてフイチン酸あるいはフイチン酸とマグネ
シウムイオンの存在下に作用せしめて5′−グアニ
ル酸を生成せしめ、生成した5′−グアニル酸を採
取することを特徴とする5′−グアニル酸の製造
法。1 Cultures, cells, or processed products of microorganisms belonging to the genus Brevibacterium or Corynebacterium and having the ability to produce 5'-guanylic acid from 5'-xanthylic acid, an ammonia donor, and an energy donor. and 5'-xanthylic acid, an ammonia donor, and an energy donor in the presence of phytic acid or phytic acid and magnesium ions in an aqueous medium containing a surfactant and/or an organic solvent to form 5'-guanyl. 1. A method for producing 5'-guanylic acid, which comprises producing an acid and collecting the produced 5'-guanylic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18705082A JPS5978697A (en) | 1982-10-25 | 1982-10-25 | Preparation of 5'-guanylic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18705082A JPS5978697A (en) | 1982-10-25 | 1982-10-25 | Preparation of 5'-guanylic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5978697A JPS5978697A (en) | 1984-05-07 |
| JPH0328196B2 true JPH0328196B2 (en) | 1991-04-18 |
Family
ID=16199293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18705082A Granted JPS5978697A (en) | 1982-10-25 | 1982-10-25 | Preparation of 5'-guanylic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5978697A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2209249C2 (en) * | 2000-11-22 | 2003-07-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Method for preparing xanthosine 5'-monophosphate, strain corynebacterium ammoniagenes as producer of xanthosine 5'-monophosphate (variants) |
-
1982
- 1982-10-25 JP JP18705082A patent/JPS5978697A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5978697A (en) | 1984-05-07 |
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