JPH03274461A - Immunological sensing method - Google Patents
Immunological sensing methodInfo
- Publication number
- JPH03274461A JPH03274461A JP7606390A JP7606390A JPH03274461A JP H03274461 A JPH03274461 A JP H03274461A JP 7606390 A JP7606390 A JP 7606390A JP 7606390 A JP7606390 A JP 7606390A JP H03274461 A JPH03274461 A JP H03274461A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- explosives
- antigen
- filter
- explosive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000001900 immune effect Effects 0.000 title description 2
- 239000002360 explosive Substances 0.000 claims abstract description 34
- 239000011148 porous material Substances 0.000 claims abstract description 3
- SPSSULHKWOKEEL-UHFFFAOYSA-N 2,4,6-trinitrotoluene Chemical compound CC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O SPSSULHKWOKEEL-UHFFFAOYSA-N 0.000 claims description 12
- 239000000015 trinitrotoluene Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- XTFIVUDBNACUBN-UHFFFAOYSA-N 1,3,5-trinitro-1,3,5-triazinane Chemical compound [O-][N+](=O)N1CN([N+]([O-])=O)CN([N+]([O-])=O)C1 XTFIVUDBNACUBN-UHFFFAOYSA-N 0.000 claims description 4
- IUKSYUOJRHDWRR-UHFFFAOYSA-N 2-diazonio-4,6-dinitrophenolate Chemical compound [O-]C1=C([N+]#N)C=C([N+]([O-])=O)C=C1[N+]([O-])=O IUKSYUOJRHDWRR-UHFFFAOYSA-N 0.000 claims description 4
- 239000000028 HMX Substances 0.000 claims description 4
- UZGLIIJVICEWHF-UHFFFAOYSA-N octogen Chemical compound [O-][N+](=O)N1CN([N+]([O-])=O)CN([N+]([O-])=O)CN([N+]([O-])=O)C1 UZGLIIJVICEWHF-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000020 Nitrocellulose Substances 0.000 claims description 2
- SNIOPGDIGTZGOP-UHFFFAOYSA-N Nitroglycerin Chemical group [O-][N+](=O)OCC(O[N+]([O-])=O)CO[N+]([O-])=O SNIOPGDIGTZGOP-UHFFFAOYSA-N 0.000 claims description 2
- 239000000006 Nitroglycerin Substances 0.000 claims description 2
- 229960003711 glyceryl trinitrate Drugs 0.000 claims description 2
- 229920001220 nitrocellulos Polymers 0.000 claims description 2
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims description 2
- AGUIVNYEYSCPNI-UHFFFAOYSA-N N-methyl-N-picrylnitramine Chemical group [O-][N+](=O)N(C)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O AGUIVNYEYSCPNI-UHFFFAOYSA-N 0.000 claims 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 claims 1
- 239000012488 sample solution Substances 0.000 abstract description 11
- 230000035945 sensitivity Effects 0.000 abstract description 10
- 239000000243 solution Substances 0.000 abstract description 10
- 239000000523 sample Substances 0.000 abstract description 7
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract description 3
- 239000000427 antigen Substances 0.000 abstract description 3
- 102000036639 antigens Human genes 0.000 abstract description 3
- 108091007433 antigens Proteins 0.000 abstract description 3
- 229940098773 bovine serum albumin Drugs 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 239000008055 phosphate buffer solution Substances 0.000 abstract description 3
- 239000004695 Polyether sulfone Substances 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 abstract description 2
- 229920006393 polyether sulfone Polymers 0.000 abstract description 2
- 210000002966 serum Anatomy 0.000 abstract description 2
- 210000000952 spleen Anatomy 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 7
- 238000004817 gas chromatography Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000975 dye Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 238000002795 fluorescence method Methods 0.000 description 2
- 230000016784 immunoglobulin production Effects 0.000 description 2
- -1 1-tetryl Chemical compound 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
Abstract
Description
【発明の詳細な説明】
産業上の利用分野
本発明は 大気中に極微量に存在する有機化合物である
爆薬類を分析・検知するための方法に関する。とく番ミ
最近盛んになった爆薬類によるテロ行へ 航空機な
どの乗っ取り事件などの防止・抑制に貢献できるので、
産業分野で広く利用でき従来の技術
爆発物検知・探知のための機械装置として（よ質量分析
装置 ガスクロマログラフ装置 あるいはＸ線装置など
が一部用いられている。DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a method for analyzing and detecting explosives, which are organic compounds that exist in trace amounts in the atmosphere. Tokubanmi We can contribute to the prevention and control of terrorist attacks using explosives, which have recently become popular, and the hijacking of aircraft, etc.
Conventional techniques that can be widely used in the industrial field include mass spectrometers, gas chromatography devices, and X-ray devices.
他の方法として、熱中性子方式 化学蛍光方式などが米
国で開発されていも
発明が解決しようとする課題
質量分析装置 ガスクロマトグラフ装置あるいはＸ線装
置等の１ 金属を識別できるＸ線装置以外（戴 惑嵐
迅速法 選択性（特異性）、太きまコストなどで不十分
であり、広範囲な実用に至っていな（〜
またＸ線装置は金属部分がなｔ〜 あるいは金属部分が
極端に小さいプラスチック爆弾などでは検知困難なた敦
このような用途に使い難い課題があも
従ってこのような分野には質量分析装置やガスクロマト
グラフ装置等が用いられることになる。Although other methods such as the thermal neutron method and chemical fluorescence method have been developed in the United States, the problems that the invention attempts to solve include mass spectrometry, gas chromatography, or X-ray equipment. storm
Rapid method: It is insufficient in selectivity (specificity), thickness, cost, etc., and has not been put into widespread practical use. Difficulty in Detection As a result, mass spectrometers, gas chromatographs, and the like have been used in such fields.
さらに熱中性子方式もしくは化学蛍光方式等では 低感
度 危険性及び選択性がほとんどないと言う課題と同時
に 装置が大聖 高価などの課題があも
すなわぢ 上記の既存の分析装置は何れ叡 この用途の
ために改良されて叡 測定感度には限界があり、この値
は約１０−”ｇ／ｍｌであａ さらに装置が大型で、重
いので移動が困難であり、また比較的高価であるなどの
課題を抱えている。Furthermore, the thermal neutron method or chemical fluorescence method has problems such as low sensitivity, low risk, and almost no selectivity, and problems such as high cost of the equipment. There is a limit to the measurement sensitivity, which is approximately 10-"g/ml. Furthermore, the device is large and heavy, making it difficult to move, and it is also relatively expensive. I am holding.
本発明（友 上記の測定装置が持っている測定感度 大
きさ、重量 価格などの課題を解決することを目的とす
る。The present invention aims to solve problems such as measurement sensitivity, size, weight, and price of the above-mentioned measuring device.
課題を解決するための手段
本発明は上記課題を解決するたへ 被検知空気をフィル
ター中に通し 前記被検知空気中に含有される爆薬類を
選択的に前記フィルターに付着あるいは吸着させ、前記
付着あるいは吸着した爆薬類を水を主成分とする溶液中
に溶解させて試料液とし 前記爆薬類に対する抗体を前
記試料液と混合させ、抗原抗体反応で前記爆薬類の存在
量あるいは有無を識別することを特徴とする、 免疫的
センシング方法を提案するものである。Means for Solving the Problems The present invention solves the above problems by passing air to be detected through a filter, selectively attaching or adsorbing explosives contained in the air to be detected, and removing the adhesion from the filter. Alternatively, the adsorbed explosives are dissolved in a solution containing water as a main component to prepare a sample solution, and antibodies against the explosives are mixed with the sample solution, and the presence or absence of the explosives is identified by an antigen-antibody reaction. This paper proposes an immunosensing method characterized by:
作用
爆薬類が密層されているカバン、スーツケースなどから
漏れ出て来Ｌ あるいは密層中にカバンなどの外側表面
に付着している爆発性化合物粒子、あるいはそれから放
出される蒸気を、その周囲の空気とともにフィルターへ
送り込んで試料を捕集し これを水を主成分とする溶液
に溶解させて、この液の組成を免疫化学的分析方法で測
定する。Explosive compound particles leaking from bags, suitcases, etc. that are packed with active explosives, or particles of explosive compounds that are attached to the outer surface of bags, etc., or vapors released from them, should be removed from the surrounding area. The sample is collected by sending it into a filter along with air, which is then dissolved in a solution whose main component is water, and the composition of this solution is measured using an immunochemical analysis method.
すなわ板 検知すべき爆薬をタンパク質と化学結合させ
、これを動物に免疫して、上記爆薬に対する抗体を得も
ついで、これと前記試料液を混合させて抗原抗体反応
をさせも
この方法により、空気中に微量存在する爆薬類を高感度
＆ミ 特異的く また比較的小型軽量な装置で分析・検
知することができる。In other words, by this method, the explosive to be detected is chemically bonded to a protein, an animal is immunized with this to obtain an antibody against the explosive, and then this is mixed with the sample solution to cause an antigen-antibody reaction. It is possible to analyze and detect trace amounts of explosives in the air with high sensitivity and specificity, and with a relatively small and lightweight device.
実施例
本発明は免疫的検出方法であるたべ 選択性に優れ有用
に抗原抗体反応が進行するが、この進行の程度を、蛍光
強度の変化より、分析・検知すると、より高感度化と高
速化が計れるため好ましし〜本発明の測定方法の概要を
第１図に基づき、実施例を参照して説明する。EXAMPLE The present invention is an immunological detection method.An antigen-antibody reaction progresses in a highly selective and useful manner, but if the extent of this progress is analyzed and detected from changes in fluorescence intensity, higher sensitivity and speed can be achieved. It is preferable because it allows measurement of the following: An outline of the measuring method of the present invention will be explained based on FIG. 1 and with reference to Examples.
なお分析すべき爆薬の一例として、各種の爆発物中に広
く用いられているトリニトロトルエン（ＴＮＴ）を選び
、 １辺が１ｍの立方体の密閉容器底面！；ＴＮＴ’粒
子１ｇが入っているビーカーを静かに置き、 ３日間放
置後、箱の上部から箱内の空気を、１０００ｃｃ／分の
流量で、 １分間フィルターを通して試料を採取しｆ、
：。As an example of an explosive to be analyzed, trinitrotoluene (TNT), which is widely used in various explosives, was chosen, and the bottom of a cubic sealed container with sides of 1 m! ; Gently place a beaker containing 1 g of TNT' particles, and after leaving it for 3 days, collect a sample from the top of the box by passing the air inside the box through a filter for 1 minute at a flow rate of 1000 cc/min.
:.
ビーカー中のＴＮＴ粒子は 無色 透明で、大きいもの
は直径１ｍｒｒＫ 小さいものは１μｍ以下の粒子から
なっていた なおＴＮＴ粒子は約１０％の水分を含ませ
て安全性を高めている。The TNT particles in the beaker were colorless and transparent, with the largest particles having a diameter of 1 mrrK and the smaller particles having a diameter of 1 μm or less.The TNT particles were impregnated with approximately 10% water to increase safety.
なおＴＮＴは多少揮発性があり、室温における平衡蒸気
圧は１０−’ｔｏｒｒであり、これは約１０″日ｇ／ｍ
１に相当する。Note that TNT is somewhat volatile, and its equilibrium vapor pressure at room temperature is 10-'torr, which is about 10" g/m
Corresponds to 1.
用いたフィルターの孔径は０．４５μｍであり、形状（
よ 厚さ１００μｍ、直径３ｃｍのポリエーテルスルフ
ォン製でありへ
これよりＴＮＴを溶ａさせるために 約１ｍｌのｐＨが
７．０のリン酸緩衝液（１ｍｌ）中に溶解させた 即ち
空気を約１０００ｃ ｃ通して１ｍ１（ｌｃｃ）のリン
酸緩衝液に溶解したた＆１０”倍に濃縮したことになム
また 抗体作製のためにウシ血清アルブミン（ＢＳＡ）
を公知の方法で結合し マウスにこの抗原を約５週間免
疫して、血清よりポリクローナル抗体を作製し九
さらく 公知の方法により、ポリクローナル抗体より信
頼性が高いモノクローナル抗体を、マウスの膵臓とガン
細胞を細胞融合させて作製したつぎ！二 上記モノクロ
ーナル抗体液と上記試料液を混合して、免疫的測定法に
より、測定したこの場合の測定方法として、抗体自身の
蛍光強度の減少を利用し九
すなわぢ これらの液を内径的１ｍｍのフッ素樹脂製の
チューブにより、試料液と抗体液とをそれぞれ蛍光測定
用光学セルに約１ｍｌ／分の流速で導き、反応させた
これに波長２８０ｎｍ （バンドパス５ｎｍ）の励起光
を照射し この時に発せられる蛍光の強度を波長３４０
ｎｍ （バンドパス１０ｎｍ） テ測定し九まず、第１
図の試料液の代わりに試料が全く含まれていないリン酸
緩衝液を流し 蛍光強度を測定した その結果 強度的
４５の蛍光を発した（第２＠ ａ部分）。The pore size of the filter used was 0.45 μm, and the shape (
It is made of polyethersulfone with a thickness of 100 μm and a diameter of 3 cm.In order to dissolve TNT, it was dissolved in about 1 ml of a phosphate buffer solution with a pH of 7.0 (1 ml), that is, about 1000 μm of air. For antibody production, bovine serum albumin (BSA) was dissolved in 1 ml (lcc) of phosphate buffer and concentrated by 10".
Using a known method, mice were immunized with this antigen for about 5 weeks, and polyclonal antibodies were prepared from serum. The next one created by fusion of cells! (2) The above monoclonal antibody solution and the above sample solution were mixed and measured by an immunoassay method.The measurement method in this case was to take advantage of the decrease in the fluorescence intensity of the antibody itself. The sample solution and antibody solution were each introduced into an optical cell for fluorescence measurement at a flow rate of about 1 ml/min using a fluororesin tube, and the reacted samples were irradiated with excitation light with a wavelength of 280 nm (bandpass 5 nm). The intensity of the fluorescence emitted at a wavelength of 340
nm (bandpass 10 nm)
Instead of the sample solution shown in the figure, a phosphate buffer solution containing no sample was poured and the fluorescence intensity was measured.As a result, fluorescence with an intensity of 45 was emitted (Part 2 @ part a).
つぎに実際の試料として、上記の試料液を使用し九 す
ると、蛍光消光が起こり、蛍光強度が３０に減少した
この消光反応は約５秒で平衡状態に達した（第２図のｂ
部分）。Next, when the above sample solution was used as an actual sample, fluorescence quenching occurred and the fluorescence intensity decreased to 30%.
This quenching reaction reached an equilibrium state in about 5 seconds (b in Figure 2).
part).
この濃縮溶液中のＴＮＴ濃度を知るために 既存技術で
あるガスクロマトグラフィ （ＧＣ）を使用した 但し
この溶液のままでは感度不足で測定不可能であったので
、この液と同様な条件で作製した１００ｍ１を１ｍＨミ
水を蒸発させることにより、約１００倍に濃縮して測
定した その結果 ＧＣの感度の限界に相当する約１０
−’ｇ／ｍｌであった（測定所要時間は２０分間）。In order to find out the TNT concentration in this concentrated solution, we used gas chromatography (GC), an existing technology. However, since it was impossible to measure the TNT concentration in this concentrated solution due to lack of sensitivity, we prepared a 100ml sample under the same conditions as this solution. By evaporating water to 1 mH, the water was concentrated approximately 100 times and measured.The result was approximately 10 mH, which corresponds to the sensitivity limit of GC.
-'g/ml (measurement time required: 20 minutes).
そこで蒸発による濃縮前の試料液濃度ｉｔ ｔｏ−’
ｇ／ｍｌとなる。Therefore, the sample liquid concentration before concentration by evaporation it to-'
g/ml.
すなわぢ 従来技術のＧＣでは大気中の濃度に換算して
、１０”’ｇ／ｍｌに１／１，０００を掛けて１０−１
１ｇ／ｍｌ（測定所要時間約２１分）となり、−Ｘ
本発明による方法で（よ １０−”ｇ／ｍｌ （所要時
間 ２分以下）となり、 １００倍の高感度といえる。In other words, in conventional GC, the concentration in the atmosphere is calculated as 10''g/ml multiplied by 1/1,000, which is 10-1.
1 g/ml (measurement time required: approximately 21 minutes), -X
The method according to the present invention has a sensitivity of 10-''g/ml (required time: 2 minutes or less), which is 100 times more sensitive.
な杖 本実施例ではＴＮＴ以外にも測定すべき爆薬類と
して、ニトログリセリン、ニトロセルロー、１ テト
リル、ヘキソーゲン（ＲＤＸ）、オクトーゲン（ＨＭＸ
）、ジアゾジニトロフェノール（ＤＤＮＰ）、 トリジ
ネート、 ピクリン酸などで有効であっな
な抵 過塩素酸塩 硝酸塩などの無機物については こ
れらの抗体が作製できないので、本方式による測定はで
きな（−
また 一般にポリクローナル抗体より一層特異性が高く
、結合力の強いモノクローナル抗体を用いた場合に（よ
他の類似化合物は検知し難い力交さらに低濃度まで分
析・検知できも
−Ｘ モノクロナール抗体に比べ感度は劣る力丈ポリ
クローナル抗体の特異性が多少劣ることを逆に利用して
、立体構造が近い数種の爆薬を１つの抗体で同時に探索
できること、および抗体作製が容易であるなどの利点が
ポリクロナール抗体にはあも
抗原抗体反応の進行状態を測定する手段として、本実施
例では抗体自身の蛍光消光を利用した力丈公知の蛍光性
色素をあらかじめ抗体あるいは抗原に結合させておき、
この色素の蛍光強度の変化を測定しても同様の効果が得
られた
また本発明は蛍光に限定されるものではなく、例えば爆
薬類を助色団として発色する染料前駆体を有する抗体に
反応させ、発色させる方法等にも応用できも
発明の効果
本発明（よ 被検知空気をフィルター中に通し被検知空
気中に含有される爆薬類をフィルターに付着あるいは吸
着させ、付着あるいは吸着した爆薬類を水を主成分とし
た溶液中に溶解させて試料液とし この爆薬類に対する
抗体をこの試料液と混合し 抗原抗体反応で爆薬類の存
在量あるいは有無を識別する免疫的センシング方法であ
るたべ空気中に飛散している極微量の爆薬類を空気中か
ら捕集して、抗体の蛍光強度の変化を利用することによ
って、高感度で、迅速にしかも比較的小型で軽量な装置
で爆薬類を測定・検知でき、従って装置自体の価格も安
価にする効果があａIn this example, in addition to TNT, explosives to be measured include nitroglycerin, nitrocellulose, 1-tetryl, hexogen (RDX), and octogen (HMX).
), diazodinitrophenol (DDNP), toridinate, picric acid, etc. However, this method cannot be used to measure inorganic substances such as perchlorate and nitrate because these antibodies cannot be produced. When using a monoclonal antibody, which has higher specificity and stronger binding strength than a polyclonal antibody (although other similar compounds are difficult to detect, it can be analyzed and detected down to low concentrations). Polyclonal antibodies have the advantage of being able to use the somewhat inferior specificity of polyclonal antibodies to simultaneously search for several types of explosives with similar three-dimensional structures with one antibody, as well as the ease of antibody production. In this example, as a means to measure the progress of the antigen-antibody reaction, a known fluorescent dye that utilizes the fluorescence quenching of the antibody itself was bound to the antibody or antigen in advance.
A similar effect was obtained by measuring changes in the fluorescence intensity of this dye.Also, the present invention is not limited to fluorescence; for example, it reacts with antibodies having dye precursors that develop color using explosives as auxochromes. Effects of the Invention This invention can also be applied to methods such as passing the air to be detected through a filter and attaching or adsorbing the explosives contained in the air to the filter, and removing the attached or adsorbed explosives. This is an immunosensing method that uses an antigen-antibody reaction to identify the presence or absence of explosives by dissolving them in a solution containing water as a sample solution and mixing antibodies against these explosives with this sample solution. By collecting extremely small amounts of explosives scattered in the air and using changes in the fluorescence intensity of antibodies, explosives can be extracted quickly and with high sensitivity using a relatively small and lightweight device. It can be measured and detected, and therefore has the effect of reducing the cost of the device itself.
Claims (6)
気中に含有される爆薬類を選択的に前記フィルターに付
着あるいは吸着させ、前記フィルターに付着あるいは吸
着した爆薬類を水を主成分とする溶液中に溶解させて試
料液とし、前記爆薬類に対する抗体を前記試料液と混合
させ、抗原抗体反応で前記爆薬類の存在量あるいは有無
を識別することを特徴とする、免疫的センシング方法。(1) The air to be detected is passed through a filter, the explosives contained in the air to be detected are selectively attached or adsorbed to the filter, and the explosives attached to or adsorbed to the filter are converted into water as a main component. An immunosensing method comprising dissolving the explosives in a solution to obtain a sample liquid, mixing antibodies against the explosives with the sample liquid, and identifying the amount or presence of the explosives by an antigen-antibody reaction.
の範囲内のものを用いることを特徴とする、請求項１記
載の免疫的センシング方法。(2) The filter has a pore size of 0.1 to 1 μm in diameter.
2. The immunosensing method according to claim 1, wherein a method within the range of .
ことを特徴とする、請求項１記載の免疫的センシング方
法。(3) The immunosensing method according to claim 1, characterized in that a polyclonal antibody of explosives is used as the antibody.
徴とする、請求項１記載の免疫的センシング方法。(4) The immunosensing method according to claim 1, characterized in that a monoclonal antibody is used as the antibody.
るいは抗体に標識した色素の蛍光強度の変化を利用する
ことを特徴とする、請求項１記載の免疫的センシング方
法。(5) The immunosensing method according to claim 1, wherein a change in the fluorescence intensity of the antibody itself or a dye labeled with the antibody is used as the method for identifying by antigen-antibody reaction.
ニトログリセリン、ニトロセルロース、テトリル、ヘキ
ソーゲン（ＲＤＸ）、オクトーゲン（ＨＭＸ）、ジアゾ
ジニトロフェノール（ＤＤＮＰ）、トリジネート、ピク
リン酸のなかから選ばれたことを特徴とする、請求項１
記載の免疫的センシング方法。(6) As explosives, trinitrotoluene (TNT),
Claim 1, characterized in that it is selected from nitroglycerin, nitrocellulose, tetryl, hexogen (RDX), octogen (HMX), diazodinitrophenol (DDNP), toridinate, and picric acid.
The immunosensing method described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7606390A JPH03274461A (en) | 1990-03-26 | 1990-03-26 | Immunological sensing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7606390A JPH03274461A (en) | 1990-03-26 | 1990-03-26 | Immunological sensing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03274461A true JPH03274461A (en) | 1991-12-05 |
Family
ID=13594316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7606390A Pending JPH03274461A (en) | 1990-03-26 | 1990-03-26 | Immunological sensing method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03274461A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5367061A (en) * | 1993-05-06 | 1994-11-22 | Westinghouse Electric Company | Method of detecting nitrate esters |
| JP2006273379A (en) * | 2005-03-29 | 2006-10-12 | Yamanaka Sangyo Kk | Extraction bag for coffee and other beverage |
| CN103424388A (en) * | 2013-06-25 | 2013-12-04 | 哈尔滨师范大学 | TNT colorimetric and fluorescent detection probe and application method thereof |
-
1990
- 1990-03-26 JP JP7606390A patent/JPH03274461A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5367061A (en) * | 1993-05-06 | 1994-11-22 | Westinghouse Electric Company | Method of detecting nitrate esters |
| JP2006273379A (en) * | 2005-03-29 | 2006-10-12 | Yamanaka Sangyo Kk | Extraction bag for coffee and other beverage |
| JP4688142B2 (en) * | 2005-03-29 | 2011-05-25 | 山中産業株式会社 | Bag for extracting coffee and other palatable beverages |
| CN103424388A (en) * | 2013-06-25 | 2013-12-04 | 哈尔滨师范大学 | TNT colorimetric and fluorescent detection probe and application method thereof |
| CN103424388B (en) * | 2013-06-25 | 2016-06-22 | 哈尔滨师范大学 | A kind of TNT colorimetric fluorescent detection probe and application process thereof |
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