JPH0322928A - Formation and proliferation of tuberous tissue of plant of genus asparagus and proliferation of seedling - Google Patents
Formation and proliferation of tuberous tissue of plant of genus asparagus and proliferation of seedlingInfo
- Publication number
- JPH0322928A JPH0322928A JP15460589A JP15460589A JPH0322928A JP H0322928 A JPH0322928 A JP H0322928A JP 15460589 A JP15460589 A JP 15460589A JP 15460589 A JP15460589 A JP 15460589A JP H0322928 A JPH0322928 A JP H0322928A
- Authority
- JP
- Japan
- Prior art keywords
- tissue
- plant
- tuber
- asparagus
- genus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 235000005340 Asparagus officinalis Nutrition 0.000 title claims abstract description 41
- 230000035755 proliferation Effects 0.000 title abstract description 6
- 244000003416 Asparagus officinalis Species 0.000 title description 2
- 230000015572 biosynthetic process Effects 0.000 title description 2
- 241000234427 Asparagus Species 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 40
- 238000012258 culturing Methods 0.000 claims abstract description 17
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 15
- 230000001902 propagating effect Effects 0.000 claims description 13
- 230000012010 growth Effects 0.000 claims description 3
- 206010020880 Hypertrophy Diseases 0.000 claims description 2
- 230000002062 proliferating effect Effects 0.000 abstract 1
- 230000008719 thickening Effects 0.000 abstract 1
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- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はアスパラガス属植物の塊茎状組織形成及び増殖
方法、並びに、植物体及び種苗増殖方法に関する．
〔従来の技術〕
アスパラガス属植物の増殖は、従来、播種もしくは株分
けによって行われてきた．アスパラガス属植物は、雌雄
異株であり、雄株の方が品質や栽培管理の点で良いとさ
れている．さらに、遺伝的に変異の大きな作物であるた
め播種による増殖は、雌株の混入に招き、又、株毎の収
量及び品質の差異を招き、栽培上著しい問題となってい
る．また、株分けによる増殖は多くの人手と長い年月を
必要とし、効率が非常に悪く、ウイルス病などが伝染し
ていく危険性も非常に高い．
以上のような問題点を改善する目的で、近年、Ｕ織培養
による優良株クローンの大量増殖が注目されている．通
常、アスパラガスの大量増殖は、Ｍｉ織片を培養し、多
量の苗条（ｓｈｏｏｔ）を増殖させた後、各々を発根培
地に移植し、不定根の分化を経て幼苗となす．しかし、
発根率が一般的に低いために、増殖効率が低いという問
題点がある．また、組織片を培養し、カルスを形成させ
たのち、カルスより生じる不定胚を用いて大量増殖の手
法とするための試みがなされている（例えば、昭和６１
年度園芸学会秋季大会研究発表要旨ｐ２１０〜２１１、
昭和６１．１１．２３〜２５、於 琉球大学、昭和６２
年度園芸学会研究発表要旨ｐ２５４〜２５５、昭和６２
．１０．７〜９、於 九州大学）．不定胚を生長させる
ことにより、発根率が低いという問題は解決するものの
、単離した不定胚はほとんどが培養の途中でカルス化あ
るいは奇形化を引き起こすことが問題となっている．
〔発明が解決しようとする課題］
本発明者らは従来のアスパラガス属植物の組織培養方法
には前記した問題点のあることを認知した上で、従来法
とは異なる新規な方法によって組織培養してアスパラガ
ス属植物の種苗を効率よく増殖する方法について検討し
た。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for forming and propagating tuber-like tissues of plants of the genus Asparagus, and a method for propagating plants and seedlings. [Prior Art] Plants of the genus Asparagus have traditionally been propagated by seeding or division. Plants of the genus Asparagus are dioecious, and male plants are said to be better in terms of quality and cultivation management. Furthermore, since it is a crop with a large amount of genetic variation, propagation by sowing leads to the contamination of female plants and causes differences in yield and quality between plants, which poses a significant problem in cultivation. In addition, propagation by division requires many hands and a long period of time, is extremely inefficient, and has a high risk of transmitting viral diseases. In order to improve the above-mentioned problems, mass propagation of superior strain clones using U-woven culture has recently attracted attention. Usually, for mass propagation of asparagus, Mi strands are cultured, a large number of shoots are propagated, and then each shoot is transplanted to a rooting medium, and seedlings are formed through differentiation of adventitious roots. but,
The problem is that the rooting rate is generally low, so the propagation efficiency is low. In addition, attempts have been made to culture tissue pieces and form calluses, and then use somatic embryos produced from the calluses to achieve mass proliferation (for example, in 1981
Abstracts of research presentations at the Autumn Conference of the Horticultural Society p210-211,
November 23-25, 1986, Ryukyu University, 1988
Annual Horticultural Society Research Presentation Abstracts p254-255, 1988
．． 10.7-9, at Kyushu University). Although growing somatic embryos solves the problem of low rooting rate, the problem is that most isolated somatic embryos develop callus or malformation during culture. [Problems to be Solved by the Invention] The present inventors recognized that the conventional tissue culture method for Asparagus plants has the above-mentioned problems, and developed a tissue culture method using a novel method different from the conventional method. We investigated a method for efficiently propagating seeds and seedlings of plants of the genus Asparagus.
その結果、本発明者等はアスパラガス属植物を組織培養
して増殖するに際し、塊茎状組織を経由して植物体を増
殖することにより上記従来技術の問題点を良好に解消し
得ることを見出し、この新知見に基づいてさらに研究を
重ねることにより本発明を完或するに至ったものである
。したがって、本発明の方法によれば、
■ アスパラガス属植物の組織又はカルスを培養して塊
茎状組織を形成させることを特徴とするアスパラガス属
植物の塊茎状組織形戊方法、■ アスパラガス属植物の
組織がアスパラガス属植物の組織又はカルスを培養して
得た塊茎状組織であることを特徴とする■記載のアスパ
ラガス属植物の塊茎状組織形成方法、
■ ■又は■記載の方法で形成させた塊茎状組織を切断
して得た切片をさらに培養して塊茎状組織を肥大させ、
さらに必要に応じて同様の塊茎状組織切断→切片培養→
塊茎状組織肥大の増殖プロセスを繰り返すことを特徴と
するアスパラガス属植物の塊茎状組織増殖方法、
■ ■〜■の方法で得られた塊茎状組織を培養して植物
体を生育させることを特徴とするアスパラガス属植物の
植物体の増殖方法、
■ 塊茎状組織を培養して植物体を生育させる工程が、
塊茎状組織を培養して塊茎状組織を再生させ、前記塊茎
状組織を培養して植物体を生育ずる工程であることを特
徴とする■記載のアスパラガス属植物の植物体の増殖方
法、
■ ■又は■の方法で得られた植物体を馴化せしめて種
苗とすることを特徴とするアスパラガス属植物の種苗増
殖方法、
が提供される．
本発明を以下に詳しく説明する。As a result, the present inventors have found that when propagating Asparagus plants through tissue culture, the problems of the above-mentioned conventional techniques can be satisfactorily solved by propagating the plants via tuber-like tissues. Based on this new knowledge, further research has led to the completion of the present invention. Therefore, according to the method of the present invention, (1) a method for forming a tuber-like tissue of a plant of the genus Asparagus, which comprises culturing tissue or callus of a plant of the genus Asparagus to form a tuber-like tissue; The method for forming a tuber-like tissue of a plant of the genus Asparagus described in ■, the method described in ■ or ■, characterized in that the plant tissue is a tuber-like tissue obtained by culturing the tissue or callus of a plant of the genus Asparagus. The sections obtained by cutting the formed tuber-like tissue are further cultured to enlarge the tuber-like tissue,
Furthermore, if necessary, similar tuber-like tissue cutting → section culture →
A method for propagating tuber-like tissues of plants of the genus Asparagus, characterized by repeating the multiplication process of tuber-like tissue hypertrophy; A method for propagating a plant of the Asparagus genus, ■ a step of culturing tuber-like tissue and growing a plant;
The method for propagating a plant of the Asparagus genus according to (1), which is a step of culturing a tuber-like tissue to regenerate the tuber-like tissue, and culturing the tuber-like tissue to grow a plant; Provided is a method for propagating seeds and seedlings of plants of the genus Asparagus, which comprises acclimatizing the plants obtained by method (1) or (2) to produce seedlings. The invention will be explained in detail below.
本発明では、アスパラガス属に属する植物であればすべ
て使用できる。該植物として具体的には食用アスパラガ
スであるＡｓｐａｒａｇｕｓ ｏｆｆｉｃｉｎａｌｉｓ
Ｌ．ｖａｒ．ａｌｔｊｌｉｓ Ｌ．を例示でき、本発明
ではこれを用いるのが好ましい．
本発明ではまず、アスパラガス属植物の組織又はカルス
（ｃａｌｌｕｓ）が組織培養されて塊茎状組織が形成さ
れる．この場合の組織培養に用いられる組織としては胚
、茎頂、茎、擬葉、地下茎等の組織を例示できる．カル
スについては、本発明では該組織を例えば公知の培養方
法（例えば、園芸学会雑誌 第４０巻 第４号 ｌ１真
〜ｌ７頁， １９７１年）によって培養して得られるカ
ルスを用いることができる。In the present invention, any plant belonging to the genus Asparagus can be used. Specifically, the plant is Asparagus officinalis, which is an edible asparagus.
L. var. altjlis L. It is preferable to use this in the present invention. In the present invention, first, tissue or callus of a plant of the genus Asparagus is cultured to form a tuber-like tissue. Examples of tissues used for tissue culture in this case include tissues such as embryos, shoot tips, stems, pseudoleaves, and rhizomes. Regarding callus, in the present invention, callus obtained by culturing the tissue by a known culture method (for example, Horticultural Society Journal, Vol. 40, No. 4, pp. 11-17, 1971) can be used.
ここで塊茎状組織とは地下茎（クラウン）の一部が肥大
戒長し、貯蔵物質を蓄積したものをいう。Here, the tuber-like tissue refers to a part of the underground stem (crown) that has enlarged and lengthened and accumulated storage substances.
この塊茎状組織を形成させるための組織としては、上述
の組織又はカルスを組織培養して得たクラウンであるの
が好ましい。The tissue for forming this tuber-like tissue is preferably a crown obtained by culturing the above-mentioned tissue or callus.
この場合の本発明で言うところのクラウンとは全植物体
の地下茎に相当し、形態的には短縮茎にあたる貯蔵組織
であり、Ｍｉ織又はカルスを組織培養して得られる、塊
状ないしは冠状の形状をした細胞の塊であって幼芽ある
いは幼芽の基になる幼芽原基を含んでいるものを指す．
本発明の塊茎状組織形成において使用される培地は、無
機戒分及び炭素源を必須戊分とし、これに植物ホルモン
類、ビタミン類を添加し、更に必要に応してアミノ酸類
を添加した培地である。該培地の無機成分としては、窒
素、リン、カリウム、ナトリウム、カルシウム、マグネ
シウム、イオウ、鉄、マンガン、亜鉛、ホウ素、モリブ
デン、塩素、ヨウ素、コバルト等の元素を含む無機塩を
あげることができ、具体的には、硝酸カリウム、硝酸ナ
トリウム、硝酸アンモニウム、塩化カリウム、塩化カル
シウム、リン酸１水素カリウム、リン酸２水素ナトリウ
ム、硫酸マグネシウム、塩化マグネシウム、硫酸ナトリ
ウム、硫酸第１銖、硫酸第２鉄、硫酸マンガン、硫酸銅
、モリブデン酸ナトリウム、三酸化モリブデン、ヨウ化
カリウム、硫酸亜鉛、ホウ酸、塩化コバルト等の化合物
を例示できる。In this case, the crown as used in the present invention corresponds to the underground stem of the whole plant, and is a storage tissue that is morphologically equivalent to a shortened stem, and has a lump-like or crown-like shape obtained by tissue culturing Mi weave or callus. It refers to a mass of cells that contain buds or bud primordia that form the basis of buds. The medium used for tuber-like tissue formation of the present invention contains inorganic substances and carbon sources as essential components, to which plant hormones and vitamins are added, and amino acids are further added as necessary. It is. Inorganic components of the medium include inorganic salts containing elements such as nitrogen, phosphorus, potassium, sodium, calcium, magnesium, sulfur, iron, manganese, zinc, boron, molybdenum, chlorine, iodine, cobalt, etc. Specifically, potassium nitrate, sodium nitrate, ammonium nitrate, potassium chloride, calcium chloride, potassium monohydrogen phosphate, sodium dihydrogen phosphate, magnesium sulfate, magnesium chloride, sodium sulfate, ferrous sulfate, ferric sulfate, sulfuric acid. Examples include compounds such as manganese, copper sulfate, sodium molybdate, molybdenum trioxide, potassium iodide, zinc sulfate, boric acid, and cobalt chloride.
該培地の炭素源としては、シュクロースやグルコース等
炭水化物、その誘導体、脂肪酸などの有機酸及びエタノ
ール等の１級アルコールなどが挙げられる。Examples of carbon sources for the medium include carbohydrates such as sucrose and glucose, derivatives thereof, organic acids such as fatty acids, and primary alcohols such as ethanol.
該培地の植物ホルモンとしては、例えは、ナフタレン酢
酸（ＮＡＡ）、インドール酢酸（ｒＡＡ）、Ｐ−クロロ
フエノキシ酢酸、２．４−ジクロロフェノキシ酢酸（２
．４−Ｄ）、インドール酪酸（ＩＢＡ）、及びこれらの
誘導体等のオーキシン類及びペンジルアデニン（ＢＡ）
、カイネチン、ゼアチン等のサイトカイニン類を例示で
きる。Examples of the plant hormones in the medium include naphthalene acetic acid (NAA), indole acetic acid (rAA), P-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2
．． 4-D), indolebutyric acid (IBA), and auxins such as derivatives thereof, and penzyladenine (BA)
Examples include cytokinins such as , kinetin, and zeatin.
該培地のビタξン類としては、ビオチン、チアミン（ビ
タミン８１）、ビリドキシン（ビタミンＢ．）、ビリド
キサール、ピリドキサミン、バントテン酸カルシウム、
アスコルビン酸くビタミンＣ）、イノシトール、ニコチ
ン酸、ニコチン酸アミド及びリボフラビン（ビタミンＢ
６）などを例示できる．該培地のア主ノ酸類としては、
例えばグリシン、アラニン、グルタミン、システイン、
フエニルアラニン及びリジンなどを例示できる．
本発明の前記培地は、通常は、前記無機或分を約０．１
μＨないし約１００ｍＭ 、前記植物ホルモン類を約０
．０１■／ｌないし約１５０■／ｌ及び前記アミノ酸類
をＯないし約１０００＋ａｇ／ｊ１！含ませて使用され
ることが望ましい．
本発明に係わるＵ織培養に用いられる前記培地として具
体的には、従来から知られている植物の組織培養に用い
られている培地、例えは、ムラシゲ・スクーグ（’６２
） ［Ｍｕｒａｓｈｉｇｅ ＆ Ｓｋｏｏｇｌの培地、
リンスマイヤー・スクーグ（ＲＭ−１９６５） ［Ｌｉ
ｎｓ＠ａｉｅｒ＆ Ｓｋｏｏｇ ］の培地、ホワイト（
’６３） ［Ｗｈｉｔｅ］の培地、ガンボルグ［Ｇａｍ
ｂｏｒｇ］のＢ−５培地、三井の門９培地、二７チ・−
１− ７チの培地［Ｎｉｔｃｈ ＆ Ｎｔｔｃｈｌ等に
前記した炭素源及び植物ホルモンを添加し、更に必要に
応じて前記したビタξン類、アミノ酸類を添加して調整
された培地を例示できるが本発明ではこの中でも特にニ
ッチ・エッチ、リンスマイヤー・スクーグ、ムラシゲ・
スクーグの培地を用いて調整される培地が好ましい。上
記した従来公知の培地の組成に関しては、例えば、竹内
、中嶋、古谷著の「新植物組織培養， ｐ３８６〜ｐ３
９１朝倉書店、１９７９年に記載されている。本発明で
使用できる前記培地は液体培地又は例えば寒天やゲルラ
イトなどのゲル化剤を通常０．１〜２％含有させた固形
培地である．
本発明では前記した培地を用いてアスパラガス属植物の
組織又はカルスを組織培養して塊茎状組織を形成させ、
該塊茎状組織を組織培養して植物体を生育させるか、あ
るいは、該塊茎状１ｌ織を切断して得た切片をさらに組
織培養して塊茎状組織を肥大させ、さらに必要に応じて
同様の塊茎状組織切断→切片組織培養→塊茎状組織肥大
の増殖プロセスを繰り返すことにより増殖させた塊茎状
組織を組織培養して植物体を生育させる．塊茎状組織の
増殖には前述の塊茎状組織形成において用いる培地と同
様なものが用いられる。The vitamins in the medium include biotin, thiamine (vitamin 81), pyridoxin (vitamin B.), pyridoxal, pyridoxamine, calcium bantothenate,
ascorbic acid (vitamin C), inositol, nicotinic acid, nicotinamide and riboflavin (vitamin B
6) etc. can be exemplified. The main amino acids of the medium are:
For example, glycine, alanine, glutamine, cysteine,
Examples include phenylalanine and lysine. The medium of the present invention usually has a mineral content of about 0.1
μH to about 100mM, the plant hormones to about 0
．． 01 ■/l to about 150 ■/l and the above amino acids from O to about 1000+ag/j1! It is preferable to include it in use. Specifically, the medium used in the U-woven culture according to the present invention includes a conventionally known culture medium used in plant tissue culture, such as Murashige Skoog ('62
) [Murashige &Skoogl's medium,
Linsmeyer Skoog (RM-1965) [Li
ns@aier&Skoog] medium, white (
'63) [White] medium, Gamborg [Gam
borg]'s B-5 medium, Mitsui's Mon 9 medium, 27-chi.-
An example is a medium prepared by adding the above-mentioned carbon sources and plant hormones to Nitch & Nttchl, etc., and further adding the above-mentioned vitamins and amino acids as necessary. In terms of inventions, Niche Ecchi, Linsmeyer Skoog, and Murashige
A medium prepared using Skoog's medium is preferred. Regarding the composition of the above-mentioned conventionally known culture medium, see, for example, "New Plant Tissue Culture," by Takeuchi, Nakajima, and Furuya, p.386-p.3.
91 Asakura Shoten, 1979. The medium that can be used in the present invention is a liquid medium or a solid medium containing usually 0.1 to 2% of a gelling agent such as agar or Gelrite. In the present invention, tissue or callus of an Asparagus plant is tissue cultured using the above-mentioned medium to form a tuber-like tissue,
The tuber-like tissue is tissue cultured to grow a plant body, or the tuber-like tissue is further grown by tissue culture of the cut section obtained by cutting the tuber-like tissue, and if necessary, a similar method is grown. By repeating the multiplication process of cutting tuber-like tissue → section tissue culture → expanding tuber-like tissue, the grown tuber-like tissue is tissue cultured and a plant is grown. For the growth of tuber-like tissue, a medium similar to that used in the above-mentioned tuber-like tissue formation is used.
上記植物体の生育工程においては、塊茎状組織からクラ
ウンを再生させ、このクラウンを組織培養して植物体を
再生することもできる。In the above-mentioned plant growth process, a crown can be regenerated from a tuber-like tissue, and the crown can be tissue cultured to regenerate a plant.
上述のような植物体の生育工程には発根工程が含まれる
．
植物体を生育させるのに用いる培地としては、例えば、
ムラシゲ・スクーグ（’６２） ［Ｍｕｒａｓｈｉｇｅ
＆ Ｓｋｏｏｇｌの培地、リンスマイヤー・スクーグ（
ＲＭ−１９６５） ［Ｌｉｎｓｍａｉｅｒ ＆ Ｓｋｏ
ｏｇｌの培地、ホワイト（’ ６３） ［ Ｗｈ ｉ
ｔｅ ］の培地、ガンボルグ［Ｇａｓａｂｏｒｇ ］
のＢ−５培地、三井のＭ−９培地、ニッチ・エッチの培
地［Ｎｉｔｃｈ ＆ Ｎｉｔｃｈ ］等に前記した炭素
源及び植物ホルモンを添加し、更に必要に応じて前記し
たビタミン類、ア藁ノ酸類を添加して調整された培地が
挙げられる。The plant growth process described above includes the rooting process. Examples of media used to grow plants include:
Murashige Skoog ('62) [Murashige
&Skoogl's medium, Linsmeyer-Skoog (
RM-1965) [Linsmaier & Sko
ogl medium, white ('63) [Wh i
te] medium, Gamborg [Gasaborg]
B-5 medium of Mitsui, M-9 medium of Mitsui, Nitch & Nitch medium, etc., the carbon source and plant hormones described above are added, and if necessary, the vitamins and amino acids described above are added. An example of this is a medium prepared by adding .
さらに本発明においては前記植物体を馴化せしめること
によりアスパラガス属植物の種苗が得られる。Furthermore, in the present invention, seedlings of plants of the genus Asparagus can be obtained by acclimating the plants.
次に図面に基づいて、本発明の各方法、並びに、各方法
によって得られた塊茎状組織、植物体等について説明す
る。Next, each method of the present invention and the tuber-like tissues, plants, etc. obtained by each method will be explained based on the drawings.
第１図は、本発明の方法によるアスパラガス属植物の塊
茎状組織形成、増殖、植物体の増殖等のプロセスを示す
図である。図の上部には、苗条の茎を切断して得られた
切片をｍ織培養してクラウンが形成される経路が示され
ており、図の下部の左側には、前記クラウンから塊茎状
組織を形成し、増殖する増殖サイクルが示され、図の下
部の右側には、このようにして増殖された塊茎状組織か
ら発根、再生させた植物体が示されている。FIG. 1 is a diagram showing processes such as tuber-like tissue formation, proliferation, and plant growth of plants of the genus Asparagus according to the method of the present invention. The upper part of the figure shows the route by which a crown is formed by culturing the sections obtained by cutting the shoot stem, and the lower left part of the figure shows the path through which the tuber-like tissue is grown from the crown. The growth cycle of formation and multiplication is shown, and the lower right side of the figure shows a plant that has been rooted and regenerated from the tuber tissue grown in this way.
以下、本発明の方法を実施例によって具体的に示す。 Hereinafter, the method of the present invention will be specifically illustrated by examples.
実施例１
アスパラガスの品種“北海１００”の無菌系で維持して
いる発根苗の基部に生じたクラウンを１芽ずつ含むよう
に切り分けたものを材料とした．培地は、無機要素をＭ
Ｓ培地、有機要素をエッチ・ニッチを基本とし、ＮＨ４
ＮＯ３を１／２倍に、ＣａＣ１．を２倍とし、グルタミ
ン１０− ’Ｍを添加したものを基本培地（以後、ＡＳ
Ｐ培地と略記）とし、シ！Ｆ糖６０ｇ／ １、ゲルライ
ト ２．５ｇ／ｌ， ｐＨ５． ７とし、これにオー
キシンとしてｌＢＡを３ ＸＩＯ−’Ｍ 、サイトカイ
ニンとしてＢＡを１０−’Ｍ 、３ ＸＩＯ−”Ｍ、１
０−’Ｍ，抗ジベレリンとしてアンシミドールを１０−
’？Ｉを添加した．これを市販のマヨネーズびん（容積
約２２０ｍｌ’，に各３Ｑａｅ分注した後、上記アスパ
ラガスクラウン切片を５個置床してマヨネーズびんｌＯ
本、２５℃、３０００１ｕｘ下で８週間培養したところ
表１に示す結果を得た。Example 1 The crown of the rooting seedling of the asparagus variety "Hokkai 100" maintained in a sterile system was cut into pieces containing one bud each. The medium contains inorganic elements
S medium, organic elements based on etch/niche, NH4
NO3 was increased to 1/2, CaC1. was doubled and 10-'M glutamine was added to the basal medium (hereinafter referred to as AS
P medium) and shi! F sugar 60g/1, Gelrite 2.5g/l, pH 5. 7, and add lBA as auxin to 3 XIO-'M and BA as cytokinin to 10-'M, 3XIO-'M, 1
0-'M, 10-'M, uncimidol as anti-gibberellin.
'? I was added. After dispensing 3 Qae each into commercially available mayonnaise bottles (volume approximately 220 ml'), place 5 of the above asparagus crown slices and fill the mayonnaise bottles with 100ml.
When the cells were cultured for 8 weeks at 25° C. and 30,001 ux, the results shown in Table 1 were obtained.
実施例２
実施例ｌの手法により作或したアスパラガスの品種“北
海１００”の塊茎状組織を２〜３芽を含むように切り分
けたものを材料とした．培地はＡＳＰ培地を基本とし、
シ＝ｓ ｔ４６ｏｇ７ ｘ、ゲルライ１・７ｇ／ｉ！，
ｐＨ５．７とし、抗ジベレリンとしてアンシミドー
ルを３ ＸＩＯ−’Ｍ添加した．これを市販のマヨネー
ズびん（容積約２２０ｄ）に各３０一分注した後、上記
アスパラガス塊茎状組織切片を各５個置床してマヨネー
ズびんｌＯ本、２５゜Ｃ、３０００１ｕｘ下で８週間培
養したところ表２に示す結果を得た。Example 2 The tuber-like tissue of the asparagus cultivar "Hokkai 100" grown by the method of Example 1 was cut into pieces containing 2 to 3 buds. The medium is based on ASP medium,
Shi = s t46og7 x, gelrai 1.7g/i! ，
The pH was adjusted to 5.7, and 3 XIO-'M of ancymidol was added as an anti-gibberellin. After dispensing 30 pieces of each into commercially available mayonnaise bottles (capacity: approximately 220 d), 5 pieces of each of the above-mentioned asparagus tuber-like tissue sections were placed and cultured in 10 mayonnaise bottles at 25°C under 30,001 ux for 8 weeks. As a result, the results shown in Table 2 were obtained.
実施例３
実施例２の手法により維持しているアスパラガスの品種
“北海１００”の塊茎状組織を２〜３芽を含むように切
り分けたものを材料とした．培地はＡＳＰ培地を基本と
し、シｇＩ！１０， ３０． ６０ｇ／ ｌ、ｐｔ｛５
．８とし、抗ジベレリンとしてアンシξドールを１０一
吻添加した後、これを市販のマヨネーズびん（容積約２
２０ｍｇ，ふたにフロロカーボン製メンプランフィルタ
ー直径Ｌｏｗを一つ）にポリエステルウール約０．９ｇ
とともに各４０Ｉｄ分注した後、上記アスパラガス塊茎
状＆ｌｌ織切片を各５個置床してマヨネーズびんｌＯ本
、２５℃、３０００１ｕｘ下で８通間培養したところ表
３に示す結果を得た．表 １
表３
ＢＡ濃度
１０−”Ｍ
３Ｘ１０−”門
１０−’Ｍ
塊茎状組織形成率
２６％
５０％
４５％
シッ糖濃度
１０ｇ／ １．
３０ｇ／ １
６０ｇ／ １
発根率
１０％
３５％
４４％
表２
増殖率
４．５倍
〔発明の効果〕
本発明の方法によれば、＆ＩＩＶｊ＆培養により、アス
パラガス属植物から塊茎状組織を形成させ、また、これ
を効率的に大量増殖することができ、さらに得られた塊
茎状組織を経由して植物体及び種苗を大量に効率よく増
殖させることができる。Example 3 The tuber-like tissue of the asparagus cultivar "Hokkai 100" maintained by the method of Example 2 was cut into pieces containing 2 to 3 buds and used as a material. The medium is basically ASP medium, and SigI! 10, 30. 60g/l, pt{5
．． 8, and after adding 10 mg of Anshidole as an anti-gibberellin, this was poured into a commercially available mayonnaise bottle (capacity: approximately 2).
20mg, one fluorocarbon membrane filter diameter Low on the lid) about 0.9g of polyester wool
After dispensing 40 Id each, five of the above asparagus tuber-like &ll tissue sections were placed on a bed and cultured in 10 mayonnaise bottles at 25°C and 30,001 ux for 8 days, and the results shown in Table 3 were obtained. Table 1 Table 3 BA concentration 10-'M 3X10-'10-'M Tuberous tissue formation rate 26% 50% 45% Sitose concentration 10g/1. 30g/ 1 60g/ 1 Rooting rate 10% 35% 44% Table 2 Multiplication rate 4.5 times [Effects of the invention] According to the method of the present invention, tuber-like tissues are formed from Asparagus plants by culturing. Furthermore, this can be efficiently propagated in large quantities, and furthermore, plants and seedlings can be efficiently propagated in large quantities via the obtained tuber-like tissues.
第１図は、本発明の方法によるアスパラガス属植物の塊
茎状組織形成、増殖、植物体の増殖等のプロセスを示す
図である。
第２図は、本発明方法によって形成されたアスパラガス
の塊茎状組織を示す写真である．なお、第２図は生物の
形態を示す写真である．第
１
図FIG. 1 is a diagram showing processes such as tuber-like tissue formation, proliferation, and plant growth of plants of the genus Asparagus according to the method of the present invention. FIG. 2 is a photograph showing the tuber-like tissue of asparagus formed by the method of the present invention. Furthermore, Figure 2 is a photograph showing the morphology of living things. Figure 1
Claims (1)
茎状組織を形成させることを特徴とするアスパラガス属
植物の塊茎状組織形成方法。 ２、アスパラガス属植物の組織がアスパラガス属植物の
組織又はカルスを培養して得たクラウンであることを特
徴とする請求項１記載のアスパラガス属植物の塊茎状組
織形成方法。 ３、請求項１又は請求項２記載の方法で形成させた塊茎
状組織を切断して得た切片をさらに培養して塊茎状組織
を肥大させ、さらに必要に応じて同様の塊茎状組織切断
→切片培養→塊茎状組織肥大の増殖プロセスを繰り返す
ことを特徴とするアスパラガス属植物の塊茎状組織増殖
方法。 ４、請求項１〜３のいずれかの項記載の方法で得られた
塊茎状組織を培養して植物体を生育させることを特徴と
するアスパラガス属植物の植物体の増殖方法。 ５、塊茎状組織を培養して植物体を生育させる工程が、
塊茎状組織を培養してクラウンを再生させ、前記クラウ
ンを培養して植物体を生育する工程であることを特徴と
する請求項４記載のアスパラガス属植物の植物体の増殖
方法。 ６、植物体の生育工程に発根工程を含むことを特徴とす
る請求項４又は５記載のアスパラガス属植物の植物体の
増殖方法。 ７、請求項４乃至請求項６記載の方法で得られた植物体
を馴化せしめて種苗とすることを特徴とするアスパラガ
ス属植物の種苗増殖方法。[Scope of Claims] 1. A method for forming a tuber-like tissue of a plant of the genus Asparagus, which comprises culturing tissue or callus of a plant of the genus Asparagus to form a tuber-like tissue. 2. The method for forming a tuber-like tissue of a plant of the genus Asparagus according to claim 1, wherein the tissue of the plant of the genus Asparagus is a crown obtained by culturing tissue or callus of a plant of the genus Asparagus. 3. The sections obtained by cutting the tuber-like tissue formed by the method according to claim 1 or claim 2 are further cultured to enlarge the tuber-like tissue, and if necessary, the same tuber-like tissue is cut → A method for growing tuber-like tissue of plants of the genus Asparagus, which is characterized by repeating the growth process of section culture → tuber-like tissue hypertrophy. 4. A method for propagating a plant of the genus Asparagus, which comprises growing a plant by culturing the tuberous tissue obtained by the method according to any one of claims 1 to 3. 5. The step of culturing the tuber-like tissue and growing the plant body,
5. The method for propagating a plant of the genus Asparagus according to claim 4, comprising the steps of culturing a tuber-like tissue to regenerate a crown, and culturing the crown to grow a plant. 6. The method for propagating a plant of the genus Asparagus according to claim 4 or 5, wherein the growing step of the plant includes a rooting step. 7. A method for propagating seeds and seedlings of plants of the genus Asparagus, which comprises acclimating the plants obtained by the method according to claims 4 to 6 to produce seeds.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15460589A JPH0322928A (en) | 1989-06-19 | 1989-06-19 | Formation and proliferation of tuberous tissue of plant of genus asparagus and proliferation of seedling |
| CA 2004686 CA2004686A1 (en) | 1988-12-08 | 1989-12-06 | Method for multiplying plant belonging to the genus asparagus |
| KR1019890018140A KR900008929A (en) | 1988-12-08 | 1989-12-08 | How to grow aspargas plants |
| EP19890312806 EP0375218A3 (en) | 1988-12-08 | 1989-12-08 | Method of multiplying plant belonging to the genus asparagus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15460589A JPH0322928A (en) | 1989-06-19 | 1989-06-19 | Formation and proliferation of tuberous tissue of plant of genus asparagus and proliferation of seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0322928A true JPH0322928A (en) | 1991-01-31 |
Family
ID=15587835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15460589A Pending JPH0322928A (en) | 1988-12-08 | 1989-06-19 | Formation and proliferation of tuberous tissue of plant of genus asparagus and proliferation of seedling |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0322928A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145359A (en) * | 2015-09-15 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method for asparagus filicinus |
-
1989
- 1989-06-19 JP JP15460589A patent/JPH0322928A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145359A (en) * | 2015-09-15 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method for asparagus filicinus |
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