JPH0277499A - Detergent composition for use in automatic dishwasher - Google Patents
Detergent composition for use in automatic dishwasherInfo
- Publication number
- JPH0277499A JPH0277499A JP23083488A JP23083488A JPH0277499A JP H0277499 A JPH0277499 A JP H0277499A JP 23083488 A JP23083488 A JP 23083488A JP 23083488 A JP23083488 A JP 23083488A JP H0277499 A JPH0277499 A JP H0277499A
- Authority
- JP
- Japan
- Prior art keywords
- lipase
- weight
- alkali
- pref
- nonionic surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 15
- 239000003599 detergent Substances 0.000 title claims abstract description 9
- 102000004882 Lipase Human genes 0.000 claims abstract description 31
- 239000004367 Lipase Substances 0.000 claims abstract description 31
- 108090001060 Lipase Proteins 0.000 claims abstract description 31
- 235000019421 lipase Nutrition 0.000 claims abstract description 31
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 12
- 239000003513 alkali Substances 0.000 claims abstract description 10
- 235000019626 lipase activity Nutrition 0.000 claims abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 abstract description 14
- 210000002969 egg yolk Anatomy 0.000 abstract description 5
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- -1 polyoxyethylene Polymers 0.000 description 9
- 239000012459 cleaning agent Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 125000004432 carbon atom Chemical group C* 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 125000000217 alkyl group Chemical group 0.000 description 4
- 229920001577 copolymer Polymers 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- 235000013345 egg yolk Nutrition 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 239000004006 olive oil Substances 0.000 description 4
- 235000008390 olive oil Nutrition 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 3
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 3
- 239000013522 chelant Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000223198 Humicola Species 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 229940008309 acetone / ethanol Drugs 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 239000003945 anionic surfactant Substances 0.000 description 2
- 150000007942 carboxylates Chemical class 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920001983 poloxamer Polymers 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 101710098554 Lipase B Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 241001558145 Mucor sp. Species 0.000 description 1
- 229920002048 Pluronic® L 92 Polymers 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 229930182556 Polyacetal Natural products 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 101000968491 Pseudomonas sp. (strain 109) Triacylglycerol lipase Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002359 Tetronic® Polymers 0.000 description 1
- 241000223257 Thermomyces Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 150000001253 acrylic acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 125000003342 alkenyl group Chemical group 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000004067 bulking agent Substances 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- XJRBAMWJDBPFIM-UHFFFAOYSA-N methyl vinyl ether Chemical compound COC=C XJRBAMWJDBPFIM-UHFFFAOYSA-N 0.000 description 1
- 239000010446 mirabilite Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000005518 polymer electrolyte Substances 0.000 description 1
- 229920006324 polyoxymethylene Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- RSIJVJUOQBWMIM-UHFFFAOYSA-L sodium sulfate decahydrate Chemical compound O.O.O.O.O.O.O.O.O.O.[Na+].[Na+].[O-]S([O-])(=O)=O RSIJVJUOQBWMIM-UHFFFAOYSA-L 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical class [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 1
- 150000004044 tetrasaccharides Chemical class 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Landscapes
- Detergent Compositions (AREA)
Abstract
Description
【発明の詳細な説明】
産 土の
本発明は、酵素としてリパーゼを含有する自動食器洗い
機巧洗浄剤組成物に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an automatic dishwasher detergent composition containing lipase as an enzyme.
丈来夏致亙 自動食器洗い機は,食事後の片付けの簡潔さ。From summer to summer Automatic dishwashers simplify cleaning up after meals.
食器の仕上りの−良さ、あるいは洗浄による手荒れの無
さ等から,一般家庭においてもよく用いられるようにな
ってきている。Due to the good finish of the tableware and the fact that it does not cause roughness during washing, it is becoming more and more commonly used in households.
自動食器洗い機用洗浄剤に酵素を利用しようとする試み
は古くからなされており,特公昭47−20235号公
報にはアミラーゼ、プロテアーゼ、リパーゼを含有する
洗浄剤組成物が開示されている。Attempts to use enzymes in detergents for automatic dishwashers have been made for a long time, and Japanese Patent Publication No. 47-20235 discloses a detergent composition containing amylase, protease, and lipase.
しかしながら、これら酵素の中でリパーゼを含有する自
動食器洗い機用洗浄剤は、実用化には至っていない。こ
れは、食器に残留する油性汚れを分解するにはリパーゼ
は有効であるが、これまでのリパーゼはアルカリ側で失
活し、効力は発揮できないという欠点を有するためであ
る。However, cleaning agents for automatic dishwashers containing lipase among these enzymes have not yet been put into practical use. This is because while lipase is effective in decomposing oily stains remaining on tableware, conventional lipases have the disadvantage that they are deactivated in alkaline environments and cannot exhibit their effectiveness.
また、卵黄汚れが共存する場合には、市販されている洗
浄剤は発泡してしまい、洗浄力の低下や、泡によるポン
プの故障を引き起こすという欠点を有している。In addition, when egg yolk stains coexist, commercially available cleaning agents foam, which has the drawback of reducing cleaning power and causing pump failure due to the foam.
升朋が ゛ しようとする課胛
本発明は、油汚れ洗浄力に勝れ,かつ、卵黄汚れが共存
する場合においても勝れた性能を発揮する自動食器洗い
搬用洗浄剤を提供するものである。The objective of the present invention is to provide a cleaning agent for automatic dishwashing that has excellent oil stain cleaning ability and also exhibits excellent performance even when egg yolk stains coexist.
光11ソ」暖
本発明は、以下の(a)、(b)および(c)成分を含
有することを特徴とする。The present invention is characterized by containing the following components (a), (b), and (c).
(a)1重量%水溶液における曇点が35℃以下である
非イオン系界面活性剤:1〜15重量%。(a) Nonionic surfactant having a cloud point of 35° C. or less in a 1% by weight aqueous solution: 1 to 15% by weight.
(b)アル−カリビルダー二20〜70重量%。(b) 20-70% by weight of alkali builder.
(c) pH= 9におけるリパーゼ活性がpH=7に
おけるリパーゼ活性の30%以上を有するアルカリリパ
ーゼ。(c) An alkaline lipase whose lipase activity at pH=9 is 30% or more of the lipase activity at pH=7.
以下1本発明についてさらに詳細に説明する。The present invention will be explained in more detail below.
本発明において(a)成分として用いる非イオン系界面
活性剤は、その1重量%水溶液における曇点が35℃以
下、好ましくは30℃以下のものである。この様な非イ
オン系界面活性剤は従来公知の非イオン系界面活性剤の
中から適当に選ぶことができるが、一般には次のような
種類の中から選ぶのが好ましい。The nonionic surfactant used as component (a) in the present invention has a cloud point of 35° C. or lower, preferably 30° C. or lower in a 1% by weight aqueous solution. Such a nonionic surfactant can be appropriately selected from conventionally known nonionic surfactants, but it is generally preferable to select it from the following types.
(1)分子量1000〜約10000を有する、ポリオ
キシエチレンとポリオキシプロピレンとのブロック重合
体。プルロニックという商品名のちとにアメリカのワイ
アンドット社で最初に開発されたものであり、一般に「
プルロニック」と呼ばれる。(1) A block polymer of polyoxyethylene and polyoxypropylene having a molecular weight of 1,000 to about 10,000. It was first developed by the American Wyandotte Company under the trade name Pluronic, and is generally known as "
It's called "Pluronic."
(2)分子量1000〜約10000を有する、エチレ
ンジアミンのポリオキシエチレンとポリオキシプロピレ
ン付加体。テトロニックという商品名のちとにアメリカ
のワイアンドット社で最初に開発されたものであり、一
般に「テトロニック」と呼ばれる。(2) A polyoxyethylene and polyoxypropylene adduct of ethylenediamine having a molecular weight of 1,000 to about 10,000. It was first developed by the American company Wyandotte, and is commonly referred to as Tetronic.
(3)下記−船人(1)で表されるアルコールのエチレ
ンオキシド(EO)とプロピレンオキシド(PO)付加
体。(3) Ethylene oxide (EO) and propylene oxide (PO) adduct of alcohol represented by the following - Shipman (1).
R,−CH○(C,H40)、(C,H,○)、)i
・−(s )(式中、R□およびR2はC□〜cisの
アルキル基またはアルケニル基を示し、かつR□+R2
の合計の炭素数が7〜19である。また、mは5〜15
の正数であり、nは3〜15の正数であり、かつm/n
の比は3/1−1/2の範囲である。エチレンオキシド
とプロピレンオキシドの付加順序、付加形式は特に間す
ず、ランダム付加でも、ブロック付加でもよい。)
本発明で用いられる非イオン系界面活性剤の含有量は、
洗浄剤組成物中1〜15重量%、好ましくは3〜lO重
致の範囲である。非イオン系界面活性剤の含有量が1重
量%未満では良好な洗浄性能は得られず、また、15重
量%を超えると制泡が困難になり、好ましくない。35
℃より低い曇点を示すものを使用すると、さらに良好な
洗浄性能が得られる。R, -CH○(C,H40), (C,H,○), )i
・-(s) (wherein R□ and R2 represent an alkyl group or alkenyl group of C□ to cis, and R□+R2
The total number of carbon atoms is 7 to 19. Also, m is 5 to 15
is a positive number, n is a positive number from 3 to 15, and m/n
The ratio is in the range 3/1-1/2. The order and form of addition of ethylene oxide and propylene oxide may be interstitial, random addition, or block addition. ) The content of the nonionic surfactant used in the present invention is
It ranges from 1 to 15% by weight in the detergent composition, preferably from 3 to 10% by weight. If the content of the nonionic surfactant is less than 1% by weight, good cleaning performance cannot be obtained, and if it exceeds 15% by weight, foam control becomes difficult, which is not preferable. 35
Even better cleaning performance can be obtained by using one that exhibits a cloud point lower than °C.
本発明で(b)成分として用いるアルカリビルターとし
ては、メタ珪酸塩、珪酸塩、炭酸塩、炭酸水素塩などが
1種または組合せて用いられる。これらは通常ナトリウ
ム、カリウム等のアルカリ金属塩であることが好ましい
。As the alkali bilter used as component (b) in the present invention, metasilicates, silicates, carbonates, hydrogen carbonates, and the like can be used alone or in combination. These are usually preferably alkali metal salts such as sodium and potassium.
本発明で用いるアルカリビルグーの含有量は洗浄剤組成
物中20〜70重量%、好ましくは30〜60重量%の
範囲である。アルカリビルグーの含有量が20重量%未
満では良好な洗浄性能を得ることができず、また、70
重量%を超えてもそれほどの性能向上は望めず好ましく
ない。The content of alkali virgo used in the present invention is in the range of 20 to 70% by weight, preferably 30 to 60% by weight in the cleaning composition. If the content of alkaline bilgu is less than 20% by weight, good cleaning performance cannot be obtained;
Even if it exceeds the weight percentage, it is not preferable because no significant improvement in performance can be expected.
本発明において(C)成分として用いるアルカリリパー
ゼは、pl(=9におけるリパーゼ活性がpl+=7に
おけるリパーゼ活性の30%以上、好ましくは50%以
上を有するものである。pH7およびρ119における
リパーゼ活性は、以下のようにして測定される。The alkaline lipase used as component (C) in the present invention has a lipase activity at pl(=9) of 30% or more, preferably 50% or more of the lipase activity at pl+=7.The lipase activity at pH 7 and ρ119 is , is measured as follows.
リパーゼ“ Z
基質としてオリーブ油を用い、リパーゼ作用によって遊
離した脂肪酸をアルカリ滴定で定量し、その数値からリ
パーゼ活性を求める。Lipase "Z" Using olive oil as a substrate, the fatty acids liberated by lipase action are quantified by alkaline titration, and the lipase activity is determined from this value.
酵素単位としては、37℃で基質オリーブ油から1分間
に1μMの脂肪酸を遊離する酵素を1単位とする。One enzyme unit is an enzyme that releases 1 μM fatty acid per minute from the substrate olive oil at 37°C.
オリーブ油4mQと0.1Mリン酸緩衝液(pH7,0
) 4 m Qとを50ra Q容共栓三角フラスコに
正確にとり、よく混合し、37℃の恒温水槽を用い10
分間予熱する。これに試料溶液1mQを正確に加え、よ
く混合し、正確に20分間後アセトン・エタノール混合
液20+*Ωを注ぎ、ついでフェノールフタレン試液5
滴を指示薬として、0.05N水酸化ナトリウム試液で
滴定する。4mQ of olive oil and 0.1M phosphate buffer (pH 7.0)
) Accurately place 4 m Q and 50 ra Q volume in a stoppered Erlenmeyer flask, mix well, and use a constant temperature water bath at 37°C for 10 min.
Preheat for a minute. Add exactly 1 mQ of sample solution to this, mix well, and after exactly 20 minutes pour in acetone/ethanol mixed solution 20+*Ω, then phenolphthalene test solution 5
Using the drop as an indicator, titrate with 0.05N sodium hydroxide test solution.
別に、オリーブ油5fflQと0.1Mリン酸緩衝液(
pH7,0) 4 ts Qとを50m Q容共栓三角
フラスコに正確にとり、37℃、30分間加温後アセト
ン・エタノール混合液20m Qを注ぎ、ついで精製水
1++Aを正確に加え、フェタノールフタレン試液5滴
を指示薬として、 0.05N水酸化ナトリウム試液で
滴定し、対照液とする。Separately, add 5fflQ of olive oil and 0.1M phosphate buffer (
Accurately place 4 ts Q (pH 7,0) into a 50 m Q volume stoppered Erlenmeyer flask, heat at 37°C for 30 minutes, then pour in 20 m Q of the acetone/ethanol mixture, then add exactly 1++ A of purified water, and add phethanol phthalene. Using 5 drops of the test solution as an indicator, titrate with 0.05N sodium hydroxide test solution and use as a control solution.
酵素単位の計算
リパーゼ力価(単位/g)
=(試料溶液滴定値一対照液滴定値)
なお、PH=9での測定に当っては、
0.2M トリス緩衝液を用いてpH=9とし、他は上
記方法に準拠して行った。Calculation of enzyme unit Lipase titer (unit/g) = (sample solution titration value - control solution titration value) When measuring at pH = 9, use 0.2M Tris buffer to set pH = 9. , and others were carried out in accordance with the above method.
本発明に好適なアルカリリパーゼとしては、Candi
da cylindracea、 1luIIlico
la lanuginosa。As alkaline lipase suitable for the present invention, Candi
da cylindracea, 1luIIlico
la lanuginosa.
1’sudomonas、 Mucor sp、、 C
hromobacteriumviscosulI1%
Rh1zopus japonics等の微生物起源の
ものがあり、リパーゼMY、リパーゼOF(以上。1'sudomonas, Mucor sp., C
hromobacterium viscosulI 1%
There are lipases derived from microorganisms such as Rh1zopus japonics, including lipase MY and lipase OF.
四糖産業■製、起gCandida cylindra
cea)、リパーゼP、リパーゼCBS(以上、大野製
薬■製、起@ Pseudomonas) 、リパーゼ
sp(ノボ社製、起源Mucor sp、)、リパーゼ
(東洋醸造■製、起源Chron+obacteriu
m viscosum)、リパーゼLPL (オリエン
タル酵母−12)、オリバーゼ(大阪細菌研究所型、起
源Rh1zopus japonics)、リパーゼB
(サラポロビール■製、起源Pseudomonas)
などとして市販されている。また、特公昭53−453
94号公報には、フミコーラ属(サーモマイセス属も包
含する)に属するリパーゼ生産糸状菌を培養し、培養物
からリパーゼを採取する事;および、この生産菌の一例
としてフミコーラ・ランイノ−サス5−38株(微工研
菌寄第1045号)が報告されているが、このようなリ
パーゼも好適である。Candida cylindra, manufactured by Tetrasaccharide Industry ■
cea), Lipase P, Lipase CBS (manufactured by Ohno Pharmaceutical ■, Origin @ Pseudomonas), Lipase SP (manufactured by Novo Co., Ltd., Origin Mucor sp), Lipase (manufactured by Toyo Jozo ■, Origin Chron+obacteriu)
m viscosum), Lipase LPL (Oriental Yeast-12), Olivase (Osaka Bacteria Research Institute type, origin Rh1zopus japonics), Lipase B
(Made by Sara Polo Beer, origin Pseudomonas)
It is commercially available as. In addition, special public service 53-453
Publication No. 94 discloses that a lipase-producing filamentous fungus belonging to the Humicola genus (including the Thermomyces genus) is cultivated, and lipase is collected from the culture; strain (Keikoken Bibori No. 1045) has been reported, and such lipases are also suitable.
さらに、遺伝子組換え微生物から生産されるリパーゼも
使用できる。このような−例として、特開昭62−27
2988号には、アスペルギルス・オリザ(Asper
gillus oryzae)を宿主とし、組換えDN
A技術で形質転換し、これを培養基中で培養してリパー
ゼを生産することが報告されている。Furthermore, lipases produced from genetically modified microorganisms can also be used. As an example of this, JP-A-62-27
In No. 2988, Aspergillus oryza (Aspergillus oryzae)
gillus oryzae) as a host, and the recombinant DNA
It has been reported that lipase can be produced by transforming cells using the A technique and culturing them in a culture medium.
(c)成分のアルカリリパーゼは、洗浄剤1kg当り1
万〜100万リパ一ゼ単位(LU)の量で配合すること
が好適である。(c) Component alkaline lipase is 1 kg per 1 kg of cleaning agent.
It is suitable to blend in an amount of 10,000 to 1,000,000 lipase units (LU).
本発明の洗浄剤組成物には上記必須成分に加え、必要に
応じて種々の任意成分を添加することができる。任意成
分としては、(a)成分以外のアニオン界面活性剤、ノ
ニオン界面活性剤、カチオン界面活性剤などの界面活性
剤、ゼオライト、水溶性無リンカルシウム捕捉キレート
ビルダー、(c)成分以外の他の酵素、次亜塩素酸塩、
過炭酸塩等の漂白剤、亜硫酸塩等の還元剤。In addition to the above-mentioned essential components, various optional components can be added to the cleaning composition of the present invention as needed. Optional ingredients include surfactants other than component (a) such as anionic surfactants, nonionic surfactants, and cationic surfactants, zeolite, water-soluble phosphate-free calcium-trapping chelate builders, and other components other than component (c). enzymes, hypochlorite,
Bleaching agents such as percarbonates, reducing agents such as sulfites.
カルボキシメチルセルロース
リコール等の再汚染防止剤,シリコーン等の消泡剤、ホ
ワイトカーボン等の無機物質、芒硝等の増量剤,香料、
色素等の添加剤などが挙げられる。Anti-recontamination agents such as carboxymethylcellulose recall, antifoaming agents such as silicone, inorganic substances such as white carbon, bulking agents such as Glauber's salt, fragrances,
Examples include additives such as pigments.
(a)成分以外の界面活性剤のうち,アニオン界面活性
剤としては、つぎのちのが例示される。Among surfactants other than component (a), examples of anionic surfactants include the following.
・炭素数9〜18のアルキル基を有する直鎖アルキルベ
ンゼンスルホン酸塩。- Straight-chain alkylbenzene sulfonate having an alkyl group having 9 to 18 carbon atoms.
・炭素数8〜24のアルキル基を有するオレフィンスル
ホン酸塩。- Olefin sulfonate having an alkyl group having 8 to 24 carbon atoms.
・炭素数8〜16のアルキル基を有するアルキル硫酸塩
。- Alkyl sulfate having an alkyl group having 8 to 16 carbon atoms.
・炭素数8〜22の脂肪酸残基を有するαースルフォ脂
肪酸エステル塩、または脂肪酸塩。- An α-sulfo fatty acid ester salt or fatty acid salt having a fatty acid residue having 8 to 22 carbon atoms.
・エトキシ化された炭素数8〜18の1級または2級ア
ルコール硫酸塩。- Ethoxylated primary or secondary alcohol sulfate having 8 to 18 carbon atoms.
カルシウム捕捉キレートビルダーとしては。As a calcium-trapping chelate builder.
例えば、ニトリロ三酢酸塩、エチレンジアミン四酢酸塩
などのアミノポリ酢酸塩;クエン酸塩、コハク酸塩など
の多価カルボン酸塩;ポリアクリル酸塩、ヒドロキシポ
リアクリル酸塩、ポリアセタールカルボキシレート、ア
クリル酸と無水マレイン酸との共重合体の塩、無水マレ
イン酸とメチルビニルエーテルとの共重合体の塩、無水
マレイン酸とオレフィンとの共重合体の塩、アクリル酸
とメタクリル酸との共重合体の塩のような高分子電解質
などが挙げられる。For example, aminopolyacetates such as nitrilotriacetate and ethylenediaminetetraacetate; polyhydric carboxylates such as citrate and succinate; polyacrylates, hydroxypolyacrylates, polyacetal carboxylates, and acrylic acids. Salts of copolymers with maleic anhydride, salts of copolymers with maleic anhydride and methyl vinyl ether, salts of copolymers with maleic anhydride and olefins, salts of copolymers with acrylic acid and methacrylic acid Examples include polymer electrolytes such as.
上記のキレートビルダーは、それぞれ単独で用いてもよ
いし、2種以上組合せて使用してもよい。The above chelate builders may be used alone or in combination of two or more.
酵素としては、本発明のアルカリリパーゼに加え、プロ
テアーゼ、セルラーゼ、アミラーゼ等の他の酵素を添加
することができる。In addition to the alkaline lipase of the present invention, other enzymes such as protease, cellulase, amylase, etc. can be added as enzymes.
本発明の洗浄剤組成物の形態は、粉末状または粒状が好
ましい。その製造方法としては、アルカリビルダー等に
非イオン系界面活性剤を含浸させ、混合する。またはそ
れらを造粒し、さらに酵素粒子を混合する方法などが挙
げられる。The form of the cleaning composition of the present invention is preferably powder or granules. As for its manufacturing method, a nonionic surfactant is impregnated into an alkali builder or the like and mixed. Alternatively, a method may be mentioned in which they are granulated and further mixed with enzyme particles.
光見Jυケ教 、
本発明の洗浄剤組成物によれば、アルカリビルダーに、
特定性状の非イオン系界面活性剤と特定のリパーゼを組
み合わせることにより自動食器洗浄機で食器を洗浄した
場合に、優れた油汚れ洗浄力を有し、かつ、卵黄汚れが
共存する場合であっても低泡性で発泡によるトラブルを
防止できる。According to the cleaning composition of the present invention, the alkali builder contains
When dishes are washed in an automatic dishwasher by combining a nonionic surfactant with specific properties and a specific lipase, it has excellent oil stain cleaning power, and when egg yolk stains coexist. It also has low foaming properties and can prevent problems caused by foaming.
以下、実施例を挙げて本発明について更に具体的に説明
するが、これに先立って、実施例で採用した評価方法を
説明する。Hereinafter, the present invention will be described in more detail with reference to Examples. Prior to this, the evaluation method employed in the Examples will be explained.
夾1L抜盈
汚れ成分:バタ−5gを均等につけた皿4枚およびサラ
ダ油5gを均等につけ
た皿4枚を用いる。1L of dirt removed: 4 plates evenly coated with 5 g of butter and 4 plates coated evenly with 5 g of salad oil were used.
洗浄条件:ナショナル製自動食器洗浄機NP−600を
用い、標準コースで洗浄を行う。洗浄に供する食器は上
記
皿8枚とする。Washing conditions: Using a National automatic dishwasher NP-600, washing was performed using a standard course. The tableware to be washed is the 8 dishes mentioned above.
判定基準:洗浄終了後、皿8枚を取り出し、5段階評価
(汚れが完全に落ちて
いるのを5とし、全く落ちないの
を1とする)で目視判定を行い。Judgment criteria: After washing, take out the 8 plates and visually evaluate them on a 5-point scale (5 indicates that the dirt is completely removed, and 1 indicates that no dirt is removed at all).
その平均点を洗浄力とする。The average score is taken as the cleaning power.
存立5g薯
半熟卵黄20gを20cmの皿1枚に塗布し、汚れの付
着していない皿3枚および茶碗4個と共に食器洗浄機内
に設置する。洗浄機はナショナル製NP−600を用い
、標準コースで洗浄する。洗浄スタートIO分間経過後
に洗浄機の扉を開け、水面からの名高を81’J定する
。Apply 20 g of half-boiled egg yolk to one 20 cm plate and place it in a dishwasher together with 3 clean plates and 4 bowls. The washing machine used is National NP-600, and the washing was carried out in a standard course. After the cleaning start IO minutes have elapsed, open the door of the washer and set the height from the water surface to 81'J.
実施例
表−1に示した洗浄剤成分をV型混合器で混合し1粒状
洗浄剤(洗浄剤N11l〜4)を調製した。The cleaning agent components shown in Example Table 1 were mixed in a V-type mixer to prepare one granular cleaning agent (cleaning agent N11l-4).
得られた各粒状洗浄剤5gを採取し、さらに既定量にな
るように顆粒状の酵素を加え、試験に供した。酵素無添
加系と比較した試験結果を表−2に示した。5 g of each of the obtained granular detergents was collected, granular enzyme was added to a predetermined amount, and the mixture was subjected to a test. The test results compared with the enzyme-free system are shown in Table 2.
ここで、リパーゼはフミコーラ・ランギノーサスのDN
A組換えによる菌から生産されたリパーゼ〔活性比=7
0%(pH= 9 /pH= 7 ))を用いた。Here, lipase is the DN of Humicola langinosa.
Lipase produced from A recombinant bacteria [activity ratio = 7
0% (pH=9/pH=7)) was used.
表−1:粒状洗浄剤組成物
量20%、1重量%水溶液における曇点27℃、旭電化
■製×2)プルロニックL92;平均分子量3500.
1重量%水溶液における曇点26℃、旭電化@製Table-1: Particulate detergent composition amount 20%, cloud point 27°C in 1 wt% aqueous solution, Asahi Denka ■×2) Pluronic L92; average molecular weight 3500.
Cloud point of 1% by weight aqueous solution: 26°C, manufactured by Asahi Denka@
Claims (1)
ある非イオン系界面活性剤:1〜15重量%、 (b)アルカリビルダー:20〜70重量%、 (c)pH=9におけるリパーゼ活性がpH=7におけ
るリパーゼ活性の30%以上を有するアルカリリパーゼ
、 を含有することを特徴とする自動食器洗い機用洗浄剤組
成物。[Claims] 1. (a) Nonionic surfactant whose cloud point is 35°C or less in a 1% by weight aqueous solution: 1 to 15% by weight, (b) Alkali builder: 20 to 70% by weight, ( c) An alkaline lipase whose lipase activity at pH=9 is 30% or more of the lipase activity at pH=7. A detergent composition for an automatic dishwasher, comprising:
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23083488A JPH0277499A (en) | 1988-09-14 | 1988-09-14 | Detergent composition for use in automatic dishwasher |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23083488A JPH0277499A (en) | 1988-09-14 | 1988-09-14 | Detergent composition for use in automatic dishwasher |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH0277499A true JPH0277499A (en) | 1990-03-16 |
Family
ID=16914006
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP23083488A Pending JPH0277499A (en) | 1988-09-14 | 1988-09-14 | Detergent composition for use in automatic dishwasher |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0277499A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0465494A (en) * | 1990-07-04 | 1992-03-02 | Kao Corp | Cleaner composition for automatic dish washer |
JPH0472397A (en) * | 1990-07-11 | 1992-03-06 | Kao Corp | Cleaner composition for automatic dish washer |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63159498A (en) * | 1986-12-10 | 1988-07-02 | ユニリーバー・ナームローゼ・ベンノートシヤープ | Enzyme-containing tableware washing composition |
JPS63205399A (en) * | 1987-02-20 | 1988-08-24 | ライオン株式会社 | Detergent composition for automatic tableware washer |
-
1988
- 1988-09-14 JP JP23083488A patent/JPH0277499A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63159498A (en) * | 1986-12-10 | 1988-07-02 | ユニリーバー・ナームローゼ・ベンノートシヤープ | Enzyme-containing tableware washing composition |
JPS63205399A (en) * | 1987-02-20 | 1988-08-24 | ライオン株式会社 | Detergent composition for automatic tableware washer |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0465494A (en) * | 1990-07-04 | 1992-03-02 | Kao Corp | Cleaner composition for automatic dish washer |
JPH0472397A (en) * | 1990-07-11 | 1992-03-06 | Kao Corp | Cleaner composition for automatic dish washer |
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