JPH0272863A - Culture of bifidobacterium - Google Patents
Culture of bifidobacteriumInfo
- Publication number
- JPH0272863A JPH0272863A JP22143688A JP22143688A JPH0272863A JP H0272863 A JPH0272863 A JP H0272863A JP 22143688 A JP22143688 A JP 22143688A JP 22143688 A JP22143688 A JP 22143688A JP H0272863 A JPH0272863 A JP H0272863A
- Authority
- JP
- Japan
- Prior art keywords
- bifidobacterium
- rice bran
- koji
- decomposition product
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 29
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 24
- 235000009566 rice Nutrition 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 239000003125 aqueous solvent Substances 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims 1
- 238000012136 culture method Methods 0.000 claims 1
- 241000209094 Oryza Species 0.000 abstract description 23
- 238000000354 decomposition reaction Methods 0.000 abstract description 20
- 239000000047 product Substances 0.000 abstract description 18
- 241000894006 Bacteria Species 0.000 abstract description 7
- 239000000706 filtrate Substances 0.000 abstract description 7
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 239000002609 medium Substances 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 230000029087 digestion Effects 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000012258 culturing Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 240000006439 Aspergillus oryzae Species 0.000 description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 3
- 241001608472 Bifidobacterium longum Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 229940009291 bifidobacterium longum Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 235000020183 skimmed milk Nutrition 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- DJWYOLJPSHDSAL-UHFFFAOYSA-N Pantethine Natural products OCC(C)(C)C(O)C(=O)NCCC(=O)NCCSSCCNC(=O)CCNC(=O)C(O)C(C)(C)CO DJWYOLJPSHDSAL-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229960000903 pantethine Drugs 0.000 description 2
- DJWYOLJPSHDSAL-ROUUACIJSA-N pantethine Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSSCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)CO DJWYOLJPSHDSAL-ROUUACIJSA-N 0.000 description 2
- 235000008975 pantethine Nutrition 0.000 description 2
- 239000011581 pantethine Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000009127 Glutaminase Human genes 0.000 description 1
- 108010073324 Glutaminase Proteins 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229940008396 carrot extract Drugs 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- -1 for example Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、ビフィドバクテリウム菌の培養法に関する。[Detailed description of the invention] [Industrial application field] The present invention relates to a method for culturing Bifidobacterium.
ビフィドバクテリウム菌は、古くから乳幼児より成人に
至るまで、その健康維持に寄与している有用細菌である
ことが知られており、該ビフィドバクテリウム菌体もし
くはその含有物の投与によって・下痢症・便秘症をはじ
めとする消化器疾患や感染症等の予防、治療、腸内腐敗
発酵の抑制、皮膚疾患への治療等、広範囲の臨床面での
利用が試みられ、その有効性が実証されつつある。Bifidobacterium has long been known to be a useful bacterium that has contributed to maintaining the health of everyone from infants to adults. A wide range of clinical uses have been attempted, including the prevention and treatment of digestive diseases such as diarrhea and constipation and infectious diseases, suppression of intestinal putrefaction and fermentation, and treatment of skin diseases, and its effectiveness has been demonstrated. It is being proven.
従来より、ビフィドバクテリウム菌の培養は牛乳、還元
脱脂乳等を主成分とする培養培地に接種、培養していた
が、該菌の増殖促進に関し、これまで種々該閑の増殖促
進物質についての検討がなされている。Traditionally, Bifidobacterium was cultured by inoculating and culturing a culture medium mainly composed of cow's milk, reconstituted skim milk, etc.; are being considered.
上記したビフィドバクテリウム菌の増殖促進物質として
は、例えば人参エキス本体の一部であるパンテチン、あ
るいは麦芽エキス、酵母エキス等が知られている。As the above-mentioned Bifidobacterium growth-promoting substance, for example, pantethine, which is a part of the carrot extract itself, malt extract, yeast extract, etc. are known.
上記増殖促進物質として挙げられるパンテチンは、一部
のビフィドバクテリウム菌にしか増殖促進効果を示さな
いことから普遍性がなく、又麦芽エキス、酵母エキス等
は、上記の如き使用上の制限はないものの、該菌の増殖
促進効果が十分でない等の弱点を有する。Pantethine, which is mentioned as the above-mentioned growth-promoting substance, is not universal because it only shows a growth-promoting effect on some Bifidobacterium bacteria, and malt extract, yeast extract, etc. do not have the above-mentioned restrictions on use. However, it does have weaknesses, such as the fact that the effect of promoting the growth of the bacteria is not sufficient.
Ｃ問題点を解決するための手段〕
本発明者等は、上記従来技術の欠点を解決すべく、ビフ
ィドバクテリウム菌の培養法に関し鋭意検討を重ねた結
果、予め脱脂した米糠を製麹した麹を、水の存在下で酵
素分解した分解物の濾液に、ビフィドバクテリウム菌を
接種、培養すれば、原菌を著しく効率良く増殖させ得る
という新知見を得、本発明を完成した。Means for Solving Problem C] In order to solve the drawbacks of the above-mentioned conventional techniques, the present inventors have conducted extensive studies on the cultivation method of Bifidobacterium, and as a result, they have developed a method for making koji from pre-defatted rice bran. The present invention was completed based on the new finding that if Bifidobacterium is inoculated and cultured in the filtrate of the decomposed product obtained by enzymatically decomposing koji in the presence of water, the original bacteria can be grown extremely efficiently.
即ち、本発明は米糠を麹としたものに、水性溶媒を加え
て酵素分解した分解物又は該分解物を通常のビフィドバ
クテリウム菌培養培地に加えたものに、ビフィドバクテ
リウム菌を接種、培養することを特徴とするビフィドバ
クテリウム菌の培養法である。That is, the present invention involves inoculating Bifidobacterium into a decomposed product obtained by enzymatically decomposing rice bran as koji by adding an aqueous solvent, or adding the decomposed product to a normal Bifidobacterium culture medium. This is a method for culturing Bifidobacterium, which is characterized by culturing.
以下、本発明の詳細な説明する。The present invention will be explained in detail below.
先ず本発明に用いられる米糠としては、通常米糠と称さ
れるものならば何れを用いても良く、好ましくは予め米
糠を脱脂処理して得られる脱脂米糠を用いるのが培養効
率の点で好ましい。First, as the rice bran used in the present invention, any rice bran that is normally called rice bran may be used, and it is preferable to use defatted rice bran obtained by defatting rice bran in advance from the viewpoint of culture efficiency.
上記した米糠、好ましくは脱脂米糠に対し４０〜７０％
（Ｖ／Ｗ＞程度撒水し、これを１〜５ｋｇ／ｃ４・Ｇ程
度で５〜１２０分程度常程度より加圧、加熱蒸煮した後
、冷却し、これにアスペルギルス属、ムコール属、リゾ
ープス属等の糸状菌を接種し、次いで３０〜４５°Ｃ１
３０〜８０時間程度、通常の製麹管理を行なって米ｎ麹
を得る。40 to 70% of the above rice bran, preferably defatted rice bran
(Water is sprinkled to an extent of V/W>, and this is steamed under normal pressure and heat at a rate of about 1 to 5 kg/c4・G for about 5 to 120 minutes, then cooled and added to Aspergillus, Mucor, Rhizopus, etc. of filamentous fungi and then heated to 30-45°C1
Normal koji production management is carried out for about 30 to 80 hours to obtain rice n koji.
次いで米糠麹に、水、リン酸緩衝液、低濃度のアルコー
ル含有水溶液等の水性溶媒を２〜６倍量（Ｗ／Ｗ）程度
加え、前記米糠麹に含まれる糸状菌の産生ずる酵素によ
り４〜ＩＯ時間程度、４０〜７０゛Ｃ程度、酵素分解（
自己消化）を行なうか、又は前記酵素分解の際、別に用
意した酵素剤、例えばプロテアーゼ、アミラーゼ、セル
ラーゼ、ペクチナーゼ、グルタミナーゼ、ヌクレアーゼ
、フィターゼ、フォスホリパーゼ等の群より選ばれる少
なくとも１種以上の酵素剤を加え４〜１５時間程度、３
０〜７０ ”Ｃ程度の条件で分解し、米糠麹の酵素分解
物を得る。Next, an aqueous solvent such as water, a phosphate buffer, or a low-concentration alcohol-containing aqueous solution is added to the rice bran koji in an amount of 2 to 6 times (W/W), and an enzyme produced by the filamentous fungi contained in the rice bran koji is added to the rice bran koji. - About IO time, about 40-70°C, enzymatic decomposition (
or during the enzymatic decomposition, at least one enzyme selected from the group of separately prepared enzymes, such as protease, amylase, cellulase, pectinase, glutaminase, nuclease, phytase, phospholipase, etc. Add the agent and leave for about 4 to 15 hours, 3
It is decomposed under conditions of about 0 to 70''C to obtain an enzymatic decomposition product of rice bran koji.
得られた分解物はそのまま培養培地として用いて良く、
又該分解物に必要によりセライト、ラジオライト等の濾
過助剤を加えた後、通常の濾布を用いて濾過して得られ
る分解濾液を用いても良い。The obtained decomposition product can be used as a culture medium as it is,
Alternatively, a decomposition filtrate obtained by adding a filter aid such as celite or radiolite to the decomposition product, if necessary, and then filtering the resultant using an ordinary filter cloth may be used.
更に、上記した分解物もしくはその濾液を、例えば凍結
乾燥、熱風乾燥、真空乾燥等により乾燥して得られるも
のを培養培地として用いても良い。Furthermore, a culture medium obtained by drying the decomposition product or its filtrate, for example, by freeze drying, hot air drying, vacuum drying, etc., may be used as the culture medium.
又、本発明に於いては、上記した酵素分解物を、通常の
ビフィドバクテリウム菌の培養に用いらｈる培地、例え
ば脱脂乳に各種の糖、ビタミン等を添加した培地に加え
たものを培養培地として用いても良く、その際、酵素分
解物もしくはその濾液の場合、通常２〜３０％（Ｖ／Ｖ
）程度、又それらの乾燥物の場合、通常０．３〜３０％
（Ｗ／Ｖ）程度添加したものが培養培地として用いられ
る。In addition, in the present invention, the enzymatic decomposition product described above is added to a medium commonly used for culturing Bifidobacterium, for example, a medium containing skim milk to which various sugars, vitamins, etc. are added. may be used as a culture medium, and in this case, in the case of enzymatic decomposition products or their filtrate, the concentration is usually 2 to 30% (V/V
) degree, and in the case of their dried products, usually 0.3 to 30%
(W/V) is used as a culture medium.
本発明に用いられるビフィドバクテリウム菌トしては、
例えばビフィドバクテリウム・ロンガム、ビフィドバク
テリウム・インファンッ等カ好適な例として挙げられる
。The Bifidobacterium bacteria used in the present invention include:
Preferred examples include Bifidobacterium longum and Bifidobacterium infantum.
次ンこ、上記したビフィドバクテリウム菌を、前記県た
培養培地、即ち酵素分解物もしくは該分解物全通常のビ
フィドバクテリウム菌の培養培地に加えたものに、所望
の菌体量接種し、嫌気的条件下で３０〜４５°Ｃ程度、
６〜８０時間程度培養し、ビフィドバクテリウム菌の培
養物を得る。Next, the above-mentioned Bifidobacterium bacteria are inoculated into the culture medium prepared above, that is, the enzymatically decomposed product or all the decomposed products are added to a normal Bifidobacterium culture medium in a desired amount. and about 30-45°C under anaerobic conditions,
The mixture is cultured for about 6 to 80 hours to obtain a Bifidobacterium culture.
本発明によれば、ビフィドバクテリウム菌ヲ著しく効率
良く増殖させることが出来、本発明は産業上著しく有意
義である。According to the present invention, Bifidobacterium can be grown with remarkable efficiency, and the present invention is of great industrial significance.
以下、実施例により本発明を具体的に示す。Hereinafter, the present invention will be specifically illustrated by examples.
実施例１
脱脂した米糠（築野株式会社製）３０Ｉに２４ ｍｌの
水を撒水し、これを蒸煮缶でｌ ｋｇｌ ｃｒｌ−Ｇ
、 １時間蒸煮、滅菌した後冷却し、これに麹菌として
アスペルギルス・オリゼー４６０ ＦＥＲＭ ＢＰ −
９８３のフスマ培養物１！！（胞子数： １．６ Ｘ１
０６ケ／ｇ）を接種し、３７〜４０℃で４０時間製麹し
、米糠麹を得た。Example 1 24 ml of water was sprinkled on defatted rice bran (manufactured by Tsukino Co., Ltd.) 30I, and this was heated in a steamer to 1 kgl crl-G.
, steamed for 1 hour, sterilized, cooled, and added to this as Aspergillus oryzae 460 FERM BP -
983 bran culture 1! ! (Number of spores: 1.6 x1
06 seeds/g) was inoculated and koji was made at 37 to 40°C for 40 hours to obtain rice bran koji.
次いで米ｕｒｓの全量に、３倍量の１％・リン酸緩衝液
（ｐＨ６，８）を加え、６０°Ｃで５時間撹拌しつつ酵
素分解を行なって分解液を得た。Next, three times the amount of 1% phosphate buffer (pH 6,8) was added to the entire amount of rice urs, and enzymatic decomposition was performed while stirring at 60°C for 5 hours to obtain a decomposition solution.
上記分解液に、予めブリックスリバー培地で４８時間液
体培養したビフィドバクテリウム・ロンガムＡＴＣＣ１
５７０７０，１ｍｅ （菌体数： ７，２ ＸＩＯ２ケ
／ｍ／）を添加した後、４８時間、嫌気的に３６〜３８
°Ｃで液体培養しビフィドバクテリウム菌の培養物（菌
体数： ２．３ Ｘ １０４ケ／屑ｅ）を得た。Bifidobacterium longum ATCC1, which had been liquid cultured in Brix River medium for 48 hours, was added to the above decomposition solution.
After adding 57070.1me (number of bacterial cells: 7.2
A culture of Bifidobacterium was obtained by liquid culture at °C (number of bacterial cells: 2.3 x 104 cells/e of scraps).
実施例２
脱脂した米糠（築野株式会社製） １２ ｋｇに７．２
ｋｇの水を撒水し、１２１°Ｃ、ｌ ｋｇ／ｃＪ−Ｇで
４０分間蒸煮、殺菌し、冷却後、これに麹菌としてアス
ペルギルス・オリゼー４６０ ＦＥＲＭ ＢＰ−９８３
のフスマ培養物２５ｇ（胞子数： １．６ ＸＩＯ’ゲ
／、５１）を接種し、３７〜４０°Ｃで４０時間製麹し
て米糠麹１３ｋｇを得た。Example 2 Defatted rice bran (manufactured by Tsukino Co., Ltd.) 7.2 kg per 12 kg
kg of water was sprinkled on it, and it was sterilized by steaming for 40 minutes at 121°C and l kg/cJ-G. After cooling, Aspergillus oryzae 460 FERM BP-983 was added to this as a koji mold.
25 g of bran culture (spore count: 1.6 XIO'ge/, 51) was inoculated and koji was made at 37 to 40°C for 40 hours to obtain 13 kg of rice bran koji.
次いで上記米糠麹の全量に、３倍量の蒸留水を加え、５
８°Ｃで５時間撹拌しつつ酵素分解し、分解液を得た。Next, add 3 times the amount of distilled water to the total amount of rice bran koji, and add 5 times the amount of distilled water.
Enzymatic decomposition was performed while stirring at 8°C for 5 hours to obtain a decomposition solution.
該分解液に、セライトを濾過助剤として加えて濾布を用
いて濾過し、濾液を得、次いで該濾液を常法により凍結
乾燥して乾燥物１．８＆ｇを得た。Celite was added to the decomposition liquid as a filter aid, and the mixture was filtered using a filter cloth to obtain a filtrate.The filtrate was then freeze-dried by a conventional method to obtain 1.8 g of a dry product.
次に前述の酵素分解液の乾燥物（本発明）又は第１表に
示す添加物（対照−２〜対照−５）を基本培地〔２％（
Ｗ／Ｖ ）脱脂粉乳にシスティン・塩酸を０．０３％（
Ｗ／Ｖ ）を添加した通常のビフィドバクテリウム菌の
培養培地（ｐＨ６，８） ）に添加した培地に、予めブ
リックスリバー培地で４８時間液体培養したビフィドバ
クテリウム・ロンガム ＡＴＣＣ１５７０７を夫々０．
１ｍ／（菌体数ニア、２ ＸＩＯ２ゲ／Ｍ／）づつ、添
加した後３７°Ｃで２１時間嫌気培養してビフィドバク
テリウム菌の培養物を得た。なお、何れの試料も初発菌
数（ビフィドバクテリウム菌）は１．Ｉ ＸＩＯ２ゲ／
ｍｅにｉｔした。Next, the dried enzymatically decomposed solution (invention) or the additives shown in Table 1 (Control-2 to Control-5) were added to the basic medium [2% (
W/V) Skim milk powder with 0.03% cysteine/hydrochloric acid (
Bifidobacterium longum ATCC15707, which had been previously liquid-cultured in Brix River medium for 48 hours, was added to a normal Bifidobacterium culture medium (pH 6, 8) (W/V) (pH 6, 8).
After adding 1 m/(number of bacterial cells, 2 XIO2 g/M/), anaerobic culture was performed at 37°C for 21 hours to obtain a culture of Bifidobacterium. The initial number of bacteria (Bifidobacterium) in each sample was 1. I XIO2 game/
It happened to me.
第
表
第１表より明らかな如く、本発明方法によれば対照−１
〜対照−５に比し、乳酸等の生産性を示す酸度が著しく
上昇し、同時にビフィドバクテリウム菌数の増加も顕著
に認められた。As is clear from Table 1, according to the method of the present invention, Control-1
~Compared to Control-5, the acidity, which indicates productivity of lactic acid, etc., increased significantly, and at the same time, a significant increase in the number of Bifidobacterium bacteria was observed.
なお、第１表中増加酸度（０，Ｉ Ｎ−ＮａＯＨによる
滴定値）は、対照−１の滴定値を１．００とした場合の
各試料滴定値の比で示した。Incidentally, the increased acidity (titration value with 0,IN-NaOH) in Table 1 is shown as the ratio of the titration value of each sample when the titration value of Control-1 is set to 1.00.
Claims (1)
分解物又は該分解物を通常のビフィドバクテリウム菌培
養培地に加えたものに、ビフイドバクテリウム菌を接種
、培養することを特徴とするビフィドバクテリウム菌の
培養法。Bifidobacterium is inoculated and cultured into a decomposed product obtained by enzymatically decomposing rice bran as koji by adding an aqueous solvent, or by adding the decomposed product to a normal Bifidobacterium culture medium. Characteristic culture method of Bifidobacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22143688A JPH0272863A (en) | 1988-09-06 | 1988-09-06 | Culture of bifidobacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22143688A JPH0272863A (en) | 1988-09-06 | 1988-09-06 | Culture of bifidobacterium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0272863A true JPH0272863A (en) | 1990-03-13 |
Family
ID=16766712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22143688A Pending JPH0272863A (en) | 1988-09-06 | 1988-09-06 | Culture of bifidobacterium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0272863A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5469616A (en) * | 1990-11-15 | 1995-11-28 | Teikoku Piston Ring Co., Ltd. | Method of manufacturing a side rail of a combined oil ring |
| JP2004275189A (en) * | 2003-02-28 | 2004-10-07 | Nichimo Co Ltd | Method for co-cultivating a plurality of microorganisms, method for producing microorganism-derived preparation and microorganism-derived preparation |
| US20090035399A1 (en) * | 2005-03-30 | 2009-02-05 | Navam Hettiarachchy | Yeast Fermentation of Rice Bran Extracts |
-
1988
- 1988-09-06 JP JP22143688A patent/JPH0272863A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5469616A (en) * | 1990-11-15 | 1995-11-28 | Teikoku Piston Ring Co., Ltd. | Method of manufacturing a side rail of a combined oil ring |
| JP2004275189A (en) * | 2003-02-28 | 2004-10-07 | Nichimo Co Ltd | Method for co-cultivating a plurality of microorganisms, method for producing microorganism-derived preparation and microorganism-derived preparation |
| US20090035399A1 (en) * | 2005-03-30 | 2009-02-05 | Navam Hettiarachchy | Yeast Fermentation of Rice Bran Extracts |
| US9179687B2 (en) * | 2005-03-30 | 2015-11-10 | Board Of Trustees Of The University Of Arkansas | Yeast fermentation of rice bran extracts |
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