JPH0240643B2 - - Google Patents
Info
- Publication number
- JPH0240643B2 JPH0240643B2 JP55052153A JP5215380A JPH0240643B2 JP H0240643 B2 JPH0240643 B2 JP H0240643B2 JP 55052153 A JP55052153 A JP 55052153A JP 5215380 A JP5215380 A JP 5215380A JP H0240643 B2 JPH0240643 B2 JP H0240643B2
- Authority
- JP
- Japan
- Prior art keywords
- milk
- moisturizing
- lactic acid
- acid bacteria
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 21
- 235000013336 milk Nutrition 0.000 claims description 12
- 239000008267 milk Substances 0.000 claims description 12
- 210000004080 milk Anatomy 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- 239000004909 Moisturizer Substances 0.000 claims description 11
- 230000001333 moisturizer Effects 0.000 claims description 11
- 239000004310 lactic acid Substances 0.000 claims description 10
- 235000014655 lactic acid Nutrition 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 8
- 102000035195 Peptidases Human genes 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000003020 moisturizing effect Effects 0.000 description 19
- 239000003795 chemical substances by application Substances 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 239000003906 humectant Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 6
- 239000002537 cosmetic Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000003205 fragrance Substances 0.000 description 5
- 239000006210 lotion Substances 0.000 description 5
- 239000008213 purified water Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 4
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 102000014171 Milk Proteins Human genes 0.000 description 3
- 108010011756 Milk Proteins Proteins 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 229960000458 allantoin Drugs 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 235000021239 milk protein Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 235000021355 Stearic acid Nutrition 0.000 description 2
- 241000194020 Streptococcus thermophilus Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 235000014048 cultured milk product Nutrition 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229940057995 liquid paraffin Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 2
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 2
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 2
- 235000011118 potassium hydroxide Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 239000008117 stearic acid Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- ZORQXIQZAOLNGE-UHFFFAOYSA-N 1,1-difluorocyclohexane Chemical compound FC1(F)CCCCC1 ZORQXIQZAOLNGE-UHFFFAOYSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- BGRXBNZMPMGLQI-UHFFFAOYSA-N 2-octyldodecyl tetradecanoate Chemical compound CCCCCCCCCCCCCC(=O)OCC(CCCCCCCC)CCCCCCCCCC BGRXBNZMPMGLQI-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- -1 Polyoxyethylene cetyl ether Polymers 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- SXPWTBGAZSPLHA-UHFFFAOYSA-M cetalkonium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 SXPWTBGAZSPLHA-UHFFFAOYSA-M 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008406 cosmetic ingredient Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 150000002148 esters Chemical group 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229940075507 glyceryl monostearate Drugs 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 1
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 1
- 229940073665 octyldodecyl myristate Drugs 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000020122 reconstituted milk Nutrition 0.000 description 1
- 229940109850 royal jelly Drugs 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dairy Products (AREA)
- Cosmetics (AREA)
Description
本発明は、化粧品等に配合して使用する、人間
の皮膚のための保湿剤に関するものである。
人間の皮膚上には表皮細胞における代謝の産物
が微量存在し、これが適度の保湿性を持つため、
皮膚は乾きすぎが防止され、しなやかさを保つて
いる。ところが、老化した皮膚あるいは洗剤で洗
浄した直後の皮膚には上記代謝産物が乏しく、し
たがつて十分な保湿作用が行われないため、皮膚
はひからび、荒れた感じとなる。
このように自然な保湿作用が十分でない場合、
保湿性を持つ物質を人為的に皮膚に補給して皮膚
の乾きすぎを防ぐことは早くから行われており、
通常は化粧水、乳液、クリーム、シヤンプーリン
ス等に保湿剤を配合しておくことによりこれを行
う。
化粧品に配合するための保湿剤は、空気中の湿
度が低いときは水分を揮散させずに保持し、一方
湿度が高いときも一定限度以上には水分を吸収し
ない特性を持つことが必要である。例えばグリセ
リン、プロピレングリコール、エチレングリコー
ル等は、吸湿性が強すぎて湿度が高いときにべた
つくという欠点があるから使用できない。また保
湿性能がいかにすぐれていても、人間の皮膚に有
害な物質や不快臭を持つ物質もまた使用できな
い。
これらの要件を充足する保湿剤として従来使用
されてきたものの代表例は、ロイヤルゼリー、ア
クアライザー（商品名。アミノ酸と還元糖とのエ
ステルを主成分とするもの。）、及び乳発酵物系の
ものである。このうち乳発酵物系のものは、牛乳
に乳酸菌を接種し培養して得られた培養物から遠
心分離により不溶性物質を除去した後の乳清より
なるものであつて、保湿性のほかに、緩衝作用、
雑菌増殖抑制作用など、化粧品成分としてきわめ
て好ましい性質を持つ。
本発明は、上述のような乳発酵物系保湿剤を改
良し保湿性能を一層向上させた新規な保湿剤、す
なわち牛乳をアルカリ性にしてアルカリ性タンパ
ク質分解酵素で処理したものを主成分とする培地
に乳酸菌を接種して培養し、得られた培養物より
不溶性物質を分離した残液からなる皮膚用保湿剤
を提供するものである。
本発明による保湿剤は、従来の乳発酵物系保湿
剤と同様に化粧品に配合して使用することがで
き、これを配合した化粧品は、保湿性能その他化
粧品としての総合的な性能において一段と向上し
たものとなる。
本発明による保湿剤を製造するに当り原料とす
る牛乳には脱脂乳が最も適しているが、全乳であ
つてもよい。もちろんこれらは粉乳からの還元乳
であつても差支えない。
タンパク質分解酵素による処理を行う場合、牛
乳は乳固形分濃度を４〜15％程度とし、且つPHを
８〜10に調整することが望ましい。PH調整用のア
ルカリとしてはカセイソーダ、カセイカリ等が適
当である。タンパク質分解酵素としては酸性側で
活性を有するものもあるから、乳タンパク質を加
水分解するだけならば酸性で処理してもよいわけ
であるが、処理物がアミン臭の強いものとなり、
この臭いが最終製品にも残るので、酸性タンパク
質分解酵素による酸性側での処理は好ましくな
い。
アルカリ性タンパク質分解酵素としては、アス
ペルギルス属、ペニシリウム属、シユウドモナス
属、ストレプトマイセス属、バチルス属等の微生
物に由来するもの、たとえばアスペルギルス・オ
リゼ、バチルス・ズブチリス、バチルス・サーモ
プロテオリテイカス等に由来するものが、入手も
容易で好ましい。酵素は乳固形分１ｇ当り50〜
2000単位使用し、温度35〜60℃で30分〜４時間程
度処理して、乳タンパク質を十分加水分解する。
酵素処理を終つた牛乳はそのまま乳酸菌培養の
培地としてもよいが、ブドウ糖、シヨ糖など、乳
酸菌の増殖促進に有効な糖類その他の物質を加え
ることが望ましい。
上述のようにして調製された酵素処理牛乳培地
による乳酸菌の培養は、通常の乳酸菌飲料製造の
場合と全く同様にして行うことができる。その際
接種する乳酸菌も特に限定されるものではなく、
ラクトバチルス・アシドフイルス、同ブルガリク
ス、同カゼイ、ストレプトコツカス・サーモフイ
ラスなど、任意の乳酸菌を用いてよい。
培養物は、あらかじめ酵素処理によつてタンパ
ク質が加水分解されているため、普通の牛乳の乳
酸菌培養物の場合のような乳タンパク質の凝固物
を含まず、不溶性物質はほとんどが菌体である。
これを遠心分離器を用いて分離すれば、上澄液と
して本発明の保湿剤が得られる。
化粧品その他の皮膚塗布液に用いる場合、本発
明の保湿剤は固形物として0.05〜１重量％程度含
有させることが望ましい。本発明の保湿剤の代表
的な使用例を次に示す。なお下記の例中、保湿剤
とあるのは後記実施例によつて製造した保湿剤Ａ
であり、数字は重量部を意味する。
なお、エマルジヨン型製品である例１および例
２の製造に当たつてはまず保湿剤等水性成分の混
合物と油性成分の混合物を調製し、次いで水性成
分と油性成分を撹拌しながら混合して水中油型乳
化物にする常法を採用した。本発明の保湿剤を使
用することにより乳化が困難になることはなかつ
た。
例 １
モイスニングクリーム
保湿剤 20.0
ステアリン酸 7.0
ステアリルアルコール 2.0
パラフイン 4.0
流動パラフイン 4.0
ミリスチン酸オクチルドデシル 4.0
モノステアリン酸グリセリン 4.45
ポリオキシエチレンセチルエーテル 0.5
防腐保存剤 0.3
１，３―ブチレングリコール 6.0
クエン酸ナトリウム 0.05
エデト酸二ナトリウム 0.01
香 料 微量
（精製水を加えて全量を100とする。）
例 ２
スキンミルク
ステアリン酸 2.0
セタノール 1.0
硬化油 1.0
精製ラノリン 0.5
流動パラフイン 5.0
ソルビタンモノオレエート 1.8
水酸化カリウム 0.3
アラントイン 0.1
防腐保存剤 0.2
保湿剤 10.0
ポリオキシエチレンソルビタンモノラウレー
ト（20E.O.） 0.3
香 料 微量
（精製水を加えて全量を100とする。）
例 ３
モイスチユアーローシヨン
エタノール 8.0
香 料 微量
クエン酸ナトリウム 1.3
クエン酸 0.5
保湿剤 10.0
アラントイン 0.2
グリセリン 0.5
（精製水を加えて全量を100とする。）
例 ４
モイスニングパツク
ポリビニールアルコール 13.0
エタノール 10.0
グリセリン 2.0
アラントイン 0.1
保湿剤 5.0
香 料 微量
（精製水を加えて全量を100とする。）
以下実施例を示して本発明を説明する。
実施例
脱脂粉乳12Kgを300の水に溶解し、カセイソ
ーダでPHを8.5に調整した後、アルカリ性タンパ
ク質分解酵素・プロテオリクイフエース（オリエ
ンターゼ5N，阪急共栄物産株式会社）1200万単
位を加えて55℃で１時間保持すると、乳白色が消
え、透明感ある液になつた。処理を終つた後、ブ
ドウ糖９Kgを添加し、温度を37℃に調整してから
乳酸菌（ストレプトコツカス・サーモフイルス）
のスターターを接種し、その温度で48時間培養を
行なつた。得られた培養物を4000rpmの遠心分離
機で10分間遠心分離し、ほとんど菌体のみからな
る沈殿物を除去して透明な保湿剤2460を得た
（以下これを保湿剤Ａという）。
また酵素処理を行わなかつた以外は上記と同様
にして、保湿剤Ｂを製造した。
次に保湿剤Ａ又はＢを用いて次のような配合の
化粧水を製造し、使用感の比較評価を行なつた。
保湿剤（Ａ又はＢ） 20％
エタノール ８％
香 料 0.2％
界面活性剤 微量
精製水 71.8％
検査のパネルとしては湘南地区の30〜50才の主
婦53人を用い、両化粧水を１日おきに３日ずつ、
計６日使用させた。なお両サンプルを使う順序
は、パネルを２分して各群ごとに異ならせ、順位
効果が無視できるようにした。
その結果は第１表及び第２表のとおりで、本発
明による保湿剤Ａを配合したもののほうが圧倒的
に高い評価を得た。
TECHNICAL FIELD The present invention relates to a moisturizer for human skin that is used in cosmetics and the like. There are small amounts of metabolic products in epidermal cells on human skin, which have moderate moisturizing properties.
The skin is prevented from becoming too dry and remains supple. However, aged skin or skin that has just been washed with a detergent lacks the above-mentioned metabolites and therefore does not have a sufficient moisturizing effect, making the skin look dry and rough. If the natural moisturizing effect is not sufficient,
Artificially supplying moisturizing substances to the skin to prevent it from drying out has been practiced for a long time.
Usually, this is done by adding a moisturizing agent to lotions, milky lotions, creams, shampoo rinses, etc. Moisturizers used in cosmetics must have the property of retaining moisture without evaporating when the humidity in the air is low, and at the same time not absorbing moisture beyond a certain limit even when the humidity is high. . For example, glycerin, propylene glycol, ethylene glycol, etc. cannot be used because they are too hygroscopic and become sticky when the humidity is high. Furthermore, no matter how good the moisturizing performance is, substances that are harmful to human skin or have an unpleasant odor cannot be used. Typical examples of moisturizers that have conventionally been used to meet these requirements are royal jelly, Aqualyzer (trade name, a product whose main component is an ester of amino acids and reducing sugars), and fermented milk products. It is something. Among these, fermented milk products are made from whey, which is obtained by removing insoluble substances by centrifugation from the culture obtained by inoculating and culturing lactic acid bacteria in milk, and in addition to moisturizing properties, buffering action,
It has extremely favorable properties as a cosmetic ingredient, such as its ability to inhibit bacterial growth. The present invention provides a novel moisturizing agent that improves the moisturizing performance of the milk fermentation product as described above by improving the moisturizing agent, that is, a culture medium whose main ingredient is milk that has been made alkaline and treated with an alkaline proteolytic enzyme. The present invention provides a skin moisturizer made of a residual liquid obtained by inoculating and culturing lactic acid bacteria and separating insoluble substances from the resulting culture. The moisturizing agent according to the present invention can be used in cosmetics in the same way as conventional milk fermentation moisturizing agents, and cosmetics containing the same can be further improved in moisturizing performance and other overall cosmetic performance. Become something. Skimmed milk is most suitable as the raw material for producing the humectant according to the present invention, but whole milk may also be used. Of course, these may be reconstituted milk from powdered milk. When treating milk with proteolytic enzymes, it is desirable to adjust the milk solids concentration to about 4 to 15% and the pH to 8 to 10. Caustic soda, caustic potash, etc. are suitable as the alkali for pH adjustment. Some proteolytic enzymes are active on the acidic side, so if you just want to hydrolyze milk proteins, you can treat them with acid, but the treated product will have a strong amine odor,
Since this odor remains in the final product, treatment on the acidic side with acidic proteolytic enzymes is not preferred. Examples of alkaline proteolytic enzymes include those derived from microorganisms such as Aspergillus, Penicillium, Pseudomonas, Streptomyces, and Bacillus, such as Aspergillus oryzae, Bacillus subtilis, Bacillus thermoproteoliticus, etc. This is preferable because it is easy to obtain. Enzyme content is 50~ per gram of milk solids.
Use 2000 units and process at a temperature of 35 to 60°C for about 30 minutes to 4 hours to sufficiently hydrolyze milk proteins. Enzyme-treated milk may be used as a medium for culturing lactic acid bacteria, but it is desirable to add sugars and other substances effective in promoting the growth of lactic acid bacteria, such as glucose and sucrose. Cultivation of lactic acid bacteria using the enzyme-treated milk medium prepared as described above can be carried out in exactly the same manner as in the case of ordinary lactic acid bacteria beverage production. The lactic acid bacteria to be inoculated at this time are not particularly limited,
Any lactic acid bacteria may be used, such as Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, and Streptococcus thermophilus. Since the proteins in the culture have been hydrolyzed in advance by enzymatic treatment, the culture does not contain coagulated milk proteins unlike in the case of ordinary milk lactic acid bacteria cultures, and most of the insoluble substances are bacterial cells.
If this is separated using a centrifuge, the humectant of the present invention can be obtained as a supernatant. When used in cosmetics and other skin application liquids, the moisturizing agent of the present invention is desirably contained in an amount of about 0.05 to 1% by weight as a solid substance. Typical usage examples of the humectant of the present invention are shown below. In the examples below, the term "moisturizer" refers to the moisturizer A produced in the example below.
and numbers mean parts by weight. In the production of Example 1 and Example 2, which are emulsion type products, first a mixture of an aqueous component such as a humectant and a mixture of an oil component is prepared, and then the aqueous component and the oil component are mixed with stirring and poured into water. A conventional method of making an oil-type emulsion was adopted. Emulsification did not become difficult by using the humectant of the present invention. Example 1 Moisturizing cream moisturizer 20.0 Stearic acid 7.0 Stearyl alcohol 2.0 Paraffin 4.0 Liquid paraffin 4.0 Octyldodecyl myristate 4.0 Glyceryl monostearate 4.45 Polyoxyethylene cetyl ether 0.5 Preservative 0.3 1,3-butylene glycol 6.0 Sodium citrate 0.05 Disodium edetate 0.01 Fragrance Trace amount (Add purified water to make total volume 100.) Example 2 Skin milk stearic acid 2.0 Cetol 1.0 Hydrogenated oil 1.0 Refined lanolin 0.5 Liquid paraffin 5.0 Sorbitan monooleate 1.8 Potassium hydroxide 0.3 Allantoin 0.1 Preservative 0.2 Humectant 10.0 Polyoxyethylene sorbitan monolaurate (20E.O.) 0.3 Fragrance Trace amount (Add purified water to make the total volume 100.) Example 3 Moisture Lotion Ethanol 8.0 Fragrance Trace amount Citrus Sodium acid 1.3 Citric acid 0.5 Moisturizing agent 10.0 Allantoin 0.2 Glycerin 0.5 (Add purified water to make the total volume 100.) Example 4 Moistening pack Polyvinyl alcohol 13.0 Ethanol 10.0 Glycerin 2.0 Allantoin 0.1 Moisturizing agent 5.0 Fragrance Trace amount (purified water (The total amount is 100.) The present invention will be explained below with reference to Examples. Example: Dissolve 12 kg of skim milk powder in 300 ml of water, adjust the pH to 8.5 with caustic soda, then add 12 million units of alkaline proteolytic enzyme Proteoliquiface (Orientase 5N, Hankyu Kyoei Bussan Co., Ltd.) to 55 ml of water. When kept at ℃ for 1 hour, the milky white color disappeared and the liquid became transparent. After finishing the treatment, add 9 kg of glucose, adjust the temperature to 37℃, and then add lactic acid bacteria (Streptococcus thermophilus).
The starter was inoculated and cultured at that temperature for 48 hours. The obtained culture was centrifuged for 10 minutes using a centrifuge at 4000 rpm to remove a precipitate consisting mostly of bacterial cells to obtain a transparent moisturizing agent 2460 (hereinafter referred to as moisturizing agent A). Further, a moisturizer B was produced in the same manner as above except that the enzyme treatment was not performed. Next, a lotion with the following formulation was prepared using humectant A or B, and the feeling of use was compared and evaluated. Moisturizer (A or B) 20% Ethanol 8% Fragrance 0.2% Surfactant Micro-purified water 71.8% A panel of 53 housewives aged 30 to 50 in the Shonan area were used for the test, and both lotions were applied every other day. 3 days each
It was used for a total of 6 days. The order in which both samples were used was divided into two and the order differed for each group, so that rank effects could be ignored. The results are shown in Tables 1 and 2, and the product containing moisturizer A according to the present invention received an overwhelmingly higher evaluation.
【表】【table】
【表】【table】
Claims (1)
質分解酵素で処理したものを主成分とする培地に
乳酸菌を接種して培養し、得られた培養物より不
溶性物質を分離した残液からなる皮膚用保湿剤。1. A skin moisturizer consisting of the residual liquid obtained by inoculating and culturing lactic acid bacteria in a medium whose main component is milk made alkaline and treated with an alkaline proteolytic enzyme, and insoluble substances are separated from the resulting culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5215380A JPS56147706A (en) | 1980-04-18 | 1980-04-18 | Humectant for skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5215380A JPS56147706A (en) | 1980-04-18 | 1980-04-18 | Humectant for skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56147706A JPS56147706A (en) | 1981-11-16 |
| JPH0240643B2 true JPH0240643B2 (en) | 1990-09-12 |
Family
ID=12906905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5215380A Granted JPS56147706A (en) | 1980-04-18 | 1980-04-18 | Humectant for skin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56147706A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04109645U (en) * | 1991-03-07 | 1992-09-22 | 日産デイーゼル工業株式会社 | Vehicle brake light system |
| JPH0672743U (en) * | 1993-03-25 | 1994-10-11 | 靖浩 菊池 | Front brake indicator light of car |
| JPH06298002A (en) * | 1993-02-18 | 1994-10-25 | Isato Kato | Vehicle, its brake lamp, and safety confirmation lamp |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63310811A (en) * | 1987-06-10 | 1988-12-19 | Fumiichiro Hosoda | Make-up cosmetic |
| JPH0242010A (en) * | 1988-08-01 | 1990-02-13 | Sansho Seiyaku Co Ltd | Make-up cosmetic |
-
1980
- 1980-04-18 JP JP5215380A patent/JPS56147706A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04109645U (en) * | 1991-03-07 | 1992-09-22 | 日産デイーゼル工業株式会社 | Vehicle brake light system |
| JPH06298002A (en) * | 1993-02-18 | 1994-10-25 | Isato Kato | Vehicle, its brake lamp, and safety confirmation lamp |
| JPH0672743U (en) * | 1993-03-25 | 1994-10-11 | 靖浩 菊池 | Front brake indicator light of car |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56147706A (en) | 1981-11-16 |
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