JPH0235065A - Growth and propagation inhibitor for yeast or bacteria - Google Patents
Growth and propagation inhibitor for yeast or bacteriaInfo
- Publication number
- JPH0235065A JPH0235065A JP63186553A JP18655388A JPH0235065A JP H0235065 A JPH0235065 A JP H0235065A JP 63186553 A JP63186553 A JP 63186553A JP 18655388 A JP18655388 A JP 18655388A JP H0235065 A JPH0235065 A JP H0235065A
- Authority
- JP
- Japan
- Prior art keywords
- chitosan
- yeast
- ascorbic acid
- bacteria
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 22
- 239000003112 inhibitor Substances 0.000 title claims abstract description 14
- 241000894006 Bacteria Species 0.000 title abstract description 12
- 229920001661 Chitosan Polymers 0.000 claims abstract description 38
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 34
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 23
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 19
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 13
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 230000035755 proliferation Effects 0.000 claims description 16
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 230000002401 inhibitory effect Effects 0.000 abstract description 8
- 230000006196 deacetylation Effects 0.000 abstract description 4
- 238000003381 deacetylation reaction Methods 0.000 abstract description 4
- 241000251468 Actinopterygii Species 0.000 abstract description 2
- 235000013372 meat Nutrition 0.000 abstract description 2
- 235000013311 vegetables Nutrition 0.000 abstract description 2
- 241000235389 Absidia Species 0.000 abstract 1
- 230000000845 anti-microbial effect Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 230000002070 germicidal effect Effects 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 18
- 239000007864 aqueous solution Substances 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 229920002101 Chitin Polymers 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 235000000069 L-ascorbic acid Nutrition 0.000 description 4
- 239000002211 L-ascorbic acid Substances 0.000 description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 235000011121 sodium hydroxide Nutrition 0.000 description 3
- 239000005711 Benzoic acid Substances 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 235000010233 benzoic acid Nutrition 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 230000000850 deacetylating effect Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 235000010199 sorbic acid Nutrition 0.000 description 2
- 239000004334 sorbic acid Substances 0.000 description 2
- 229940075582 sorbic acid Drugs 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical group C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000473391 Archosargus rhomboidalis Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000606125 Bacteroides Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 206010007134 Candida infections Diseases 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-isoascorbic acid Chemical compound OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 241000235036 Debaryomyces hansenii Species 0.000 description 1
- 241001235027 Elysia Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 150000000996 L-ascorbic acids Chemical class 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229960004365 benzoic acid Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000003984 candidiasis Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000002328 demineralizing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000010350 erythorbic acid Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940026239 isoascorbic acid Drugs 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 229940095574 propionic acid Drugs 0.000 description 1
- 229940007042 proteus vulgaris Drugs 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Cosmetics (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は酵母あるいは細菌の生育および増殖抑制剤に関
し、詳しくはキトサンとアスコルビン酸を有効成分とす
る酵母あるいは細菌の生育および増殖抑制剤に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a yeast or bacterial growth and proliferation inhibitor, and more particularly to a yeast or bacterial growth and proliferation inhibitor containing chitosan and ascorbic acid as active ingredients.
食品、化粧品などの抗菌剤、保存剤、殺菌剤としては安
息香酸、ソルビン酸、プロピオン酸、サリチル酸などの
多数の合成品が知られているが。Many synthetic products such as benzoic acid, sorbic acid, propionic acid, and salicylic acid are known as antibacterial agents, preservatives, and disinfectants for foods and cosmetics.
これらの合成品は人工品であるため安全性に問題を生じ
ることがあった。Since these synthetic products are artificial products, they sometimes pose safety problems.
この点を改良するために、最近、エビやカニなどの甲殻
類の殻の中に含まれるキチンを脱アセチル化して得られ
るキトサンを有効成分とじた抗菌剤や殺菌剤が提案され
ている（特開昭５９−４６２２３号。To improve this point, antibacterial and bactericidal agents containing chitosan, which is obtained by deacetylating the chitin contained in the shells of crustaceans such as shrimp and crabs, as an active ingredient have recently been proposed (especially Kaisho 59-46223.
特開昭６２−８３８７７号）。JP-A No. 62-83877).
しかしながら、このようなキトサンを主成分とした抗菌
剤や殺菌剤は、その抗菌効果が不充分であって、抗菌効
果を発現させるためには多量に用いなければならず、ま
た酵母の生育および増殖に対する抑制効果がほとんどな
いといった欠点があった・
本発明は上記従来技術の事情に鑑みてなされたものであ
って、その目的とするところは低濃度で抗菌性を示し、
しかも細菌のみならず酵母に対しても著しい生育及び増
殖抑制効果を発揮する酵母あるいは細菌の生育および増
殖抑制剤を提供することにある。However, such antibacterial agents and bactericidal agents that have chitosan as their main ingredient have insufficient antibacterial effects and must be used in large quantities in order to exhibit their antibacterial effects, and they also have a negative effect on the growth and proliferation of yeast. The present invention was made in view of the above-mentioned circumstances of the prior art, and its purpose is to exhibit antibacterial properties at low concentrations,
Moreover, it is an object of the present invention to provide a yeast or bacterial growth and proliferation inhibitor that exhibits a remarkable growth and proliferation inhibiting effect not only on bacteria but also on yeast.
本発明者等らはキトサンを主体とする酵母あるいは細菌
の生育および増殖抑制剤に関する研究を鋭意重ねた結果
、キトサンにアスコルビン酸を組み合わせると優れた相
乗効果を奏することを見い出し、本発明を完成するに至
った。As a result of intensive research into yeast or bacterial growth and proliferation inhibitors mainly composed of chitosan, the present inventors discovered that a combination of chitosan and ascorbic acid produces an excellent synergistic effect, thereby completing the present invention. reached.
即ち、本発明の酵母あるいは細菌の生育及び増殖抑制剤
はキトサンとアスコルビン酸を有効成分としたことを特
徴とする。That is, the yeast or bacterial growth and proliferation inhibitor of the present invention is characterized by containing chitosan and ascorbic acid as active ingredients.
本発明で用いるキトサンは、従来公知のものが適用でき
、例えば市販されているキチンまたは天然に存在するキ
チンを常法により脱アセチル化して得られるキトサン等
が挙げられる。後者の例としては、例えば、カニ殻を脱
灰、脱タンパクして得られたキチンを濃度４０〜５０％
の水酸化ナトリウム水溶液に浸漬し、５０〜１３０℃で
反応させた後、アルカリを除去し、次いで水洗乾燥して
得られたフレーク状、又はさらに粉砕工程を経た粉末状
のキトサンがある。As the chitosan used in the present invention, conventionally known chitosan can be used, such as chitosan obtained by deacetylating commercially available chitin or naturally occurring chitin by a conventional method. An example of the latter is, for example, chitin obtained by demineralizing and deproteinizing crab shells at a concentration of 40 to 50%.
There are chitosan in the form of flakes obtained by immersing the chitosan in an aqueous solution of sodium hydroxide, reacting at 50 to 130°C, removing the alkali, washing with water and drying, or in the form of a powder which is further subjected to a pulverization process.
キトサンは、脱アセチル化率が７０％未満のものでは溶
解性に乏しいことから少なくとも脱アセチル化率が７０
％以上であることが望ましい。Chitosan has poor solubility when the deacetylation rate is less than 70%, so chitosan must have a deacetylation rate of at least 70%.
% or more is desirable.
また、本発明においてはキトサンの分子量が小さいと細
菌、および酵母に対する生育、増殖の抑制効果が小さく
なるので、キトサンの１％水溶液の２５℃における粘度
（Ｂ型粘度計による粘度）が５０ｃｐ。Furthermore, in the present invention, if the molecular weight of chitosan is small, the effect of inhibiting the growth and proliferation of bacteria and yeast will be reduced, so the viscosity (viscosity by B-type viscometer) of a 1% aqueous solution of chitosan at 25° C. is 50 cp.
好ましくは１００ｃｐ以上のものが用いられる。Preferably, one having a value of 100 cp or more is used.
アスコルビン酸は、Ｌ−アスコルビン酸及びイソアスコ
ルビン酸が好適に使用される。このアスコルビン酸の使
用形態は、特別に制約されないが、粉末状、あるいは水
溶液として用いることが望ましい。キトサンとアルコル
ビン酸の混合比率は重量比で１：０．９〜３．０、好ま
しくは１：１．Ｏ−２，０である。As the ascorbic acid, L-ascorbic acid and isoascorbic acid are preferably used. The form in which this ascorbic acid is used is not particularly limited, but it is desirable to use it as a powder or an aqueous solution. The mixing ratio of chitosan and ascorbic acid is 1:0.9 to 3.0 by weight, preferably 1:1. It is O-2.0.
こられの成分は必須成分であり、その他の成分を更に配
合することは差支えない。These ingredients are essential ingredients, and there is no problem in further blending other ingredients.
本発明の酵母あるいは細菌の生育および増殖抑制剤は種
々の酵母及び細菌に対して有効性を発揮するが、以下に
これらの具体例を例示する。The yeast or bacterial growth and proliferation inhibitor of the present invention is effective against various yeasts and bacteria, and specific examples thereof are illustrated below.
カンジダアンビカンス、ハンセヌラスワヴエオレンス、
デバリオマイセスハンセニー、サツカロマイセスセレヴ
イシエ、サツ力ロマイセスベイリ〔細菌の例〕
スタフィロコッカスアウレウス、バチルスサブチリス、
バチルスセレウス、ストレプトコッカスフェカリス、ス
トレプトコッカスミュータンス、ニジエリシアコリ、シ
ュードモナスアエルギノーサ、プロテウスヴルガリス、
サルモネラエンテリテイデイス、バクテロイデスジンジ
パリウス、ブリバクテリウムボリモルフユーム、ピチロ
スポラムオヴール、プロピオニバクテリウムアクネス、
ロイコノストックメセンテリオイデス
本発明においては、キトサンとアスコルビン酸の混合物
の使用形態はその用途によって、粉末状、水溶液あるい
はエタノール、イソプロピルアルコール等のアルコール
含有水溶液などの種々の形態とすることができ、必要に
応じて他の成分たとえば安息香酸、ソルビン酸等の合成
系抗菌剤を添加することもできる。Candida ambicans, Hansenula swampaeolens,
Debaryomyces hansenii, Satucharomyces cerevisiae, Satucharomyces beili [Examples of bacteria] Staphylococcus aureus, Bacillus subtilis,
Bacillus cereus, Streptococcus faecalis, Streptococcus mutans, Elysia coli, Pseudomonas aeruginosa, Proteus vulgaris,
Salmonella enteritidis, Bacteroides gingiparius, Bribacterium vorimorphum, Pityrosporum ovule, Propionibacterium acnes,
Leuconostoc mesenterioides In the present invention, the mixture of chitosan and ascorbic acid can be used in various forms depending on the use, such as a powder, an aqueous solution, or an aqueous solution containing alcohol such as ethanol or isopropyl alcohol. Other components such as synthetic antibacterial agents such as benzoic acid and sorbic acid can also be added as required.
また、具体的な使用に当っては、キトサン濃度がｏ、ｏ
ｏｓ％〜３重Ｊｔ％より好適には０．０１％〜１．０重
斌〆となるよう調整しておくことが望ましい。In addition, in specific use, the chitosan concentration is o, o
It is desirable to adjust it so that it is more preferably 0.01% to 1.0 Jt%, more preferably 0.01% to 1.0 Jt%.
本発明の酵母あるいは細菌の生育および増殖抑制剤はキ
トサンとアスコルビン酸を有効成分としたことから、キ
トサン単独あるいはキトサンとたとえば酢酸や乳酸など
の酸を組合せたものに比べその抗菌作用が低濃度で発現
し、しかも細菌のみならず酵母に対しても著しい生育及
び増殖抑制作用を発揮する。従って、本発明の酵母ある
いは細菌の生育および増殖抑制剤は、例えば、エビ、カ
イ、魚の切身等の水産物の抗菌剤、しゅうまい、かまぼ
こ等の練製品の保存剤、野菜、果実等の消毒剤、肉類、
魚介類等の食品加工用設備の殺菌、消毒剤、シャンプー
・リンス等のフケ止め剤、カンジダ症の治療薬等、食品
、医薬、化粧料等の巾広い分野に好適に利用される。Since the yeast or bacterial growth and proliferation inhibitor of the present invention contains chitosan and ascorbic acid as active ingredients, its antibacterial activity is lower in concentration than chitosan alone or chitosan in combination with an acid such as acetic acid or lactic acid. Furthermore, it exerts a remarkable growth and proliferation inhibitory effect not only on bacteria but also on yeast. Therefore, the yeast or bacterial growth and proliferation inhibitor of the present invention is, for example, an antibacterial agent for marine products such as shrimp, sea bream, and fish fillets, a preservative for processed products such as shumai and kamaboko, and a disinfectant for vegetables and fruits. meat,
It is suitably used in a wide range of fields including food, medicine, cosmetics, sterilization of food processing equipment for seafood, disinfectants, anti-dandruff agents such as shampoos and conditioners, and therapeutic agents for candidiasis.
つぎに、実施例により本発明を更に詳細に説明する。な
お、以下に示す部及び％は特に断わらない限り重量基準
である。Next, the present invention will be explained in more detail with reference to Examples. Note that the parts and percentages shown below are based on weight unless otherwise specified.
実施例１
粉末状のキトサン（キトサン２部を１％酢酸水溶液１９
８部に溶解し２５℃で測定した粘度１２００ｃｐ、脱ア
セチン化度８５％）１部にＬ−アスコルビン酸１．３部
を加え１．１５％濃度のキトサント−アスコルビン酸水
溶液を調整した。１０％カセイソーダ水溶液でキトサン
水溶液のｐＨを６．０〜６．５に調整した後、 ０．４
５μのフィルターを用いて滅菌し、次いでオートクレー
ブ滅菌したミュラーヒントン培地（Ｄｉｆｋｏ Ｌａｂ
ｏｒａｔｏｒｉｅｓ製）で倍々希釈を行って表−１に示
した濃度のキトサンを含有する培地を調整した。この培
地に予め２５℃、４８時間で前々培養、前培養した表−
１に示す酵母を植え、２５℃で４８時間培養を行い、混
濁の生成を観察し、混濁を生じたものを（＋）、混濁が
生じなかったものを（−）とした。混濁を生じたものは
酵母の増殖を示し、混濁を生じなかったものは酵母が増
殖しなかったことを示す。結果を表−１に示す。Example 1 Powdered chitosan (2 parts of chitosan was mixed with 1% aqueous acetic acid solution)
A 1.15% chitosanto-ascorbic acid aqueous solution was prepared by adding 1.3 parts of L-ascorbic acid to 1 part (1200 cp of viscosity measured at 25° C. and 85% degree of deacetylation). After adjusting the pH of the chitosan aqueous solution to 6.0 to 6.5 with a 10% caustic soda aqueous solution, the pH was adjusted to 0.4.
Mueller-Hinton medium (Difko Lab) sterilized using a 5μ filter and then autoclaved.
oratories) to prepare a culture medium containing chitosan at the concentrations shown in Table 1. A table pre-cultured and pre-cultured in this medium at 25°C for 48 hours.
The yeast shown in No. 1 was planted and cultured at 25° C. for 48 hours, and the formation of turbidity was observed. Those with turbidity were rated as (+), and those with no turbidity were rated as (−). A case in which turbidity occurred indicates yeast proliferation, and a case in which turbidity did not occur indicates that yeast did not proliferate. The results are shown in Table-1.
比較例１
実施例１で使用したキトサン１部に酢酸１部を加え０．
５％濃度のキトサン／酢酸水溶液を調整した。Comparative Example 1 1 part of acetic acid was added to 1 part of chitosan used in Example 1, and 1 part of acetic acid was added.
A 5% concentration chitosan/acetic acid aqueous solution was prepared.
以下、実施例１と同様の方法で行った。結果を表−１に
示す。The following procedure was carried out in the same manner as in Example 1. The results are shown in Table-1.
比較例２
実施例１で使用したキトサン１部に乳酸１．３部を加え
０．５％濃度のキトサン／乳酸水溶液を調整した。Comparative Example 2 1.3 parts of lactic acid was added to 1 part of chitosan used in Example 1 to prepare a 0.5% chitosan/lactic acid aqueous solution.
結果を表−１に示す。以下、実施例１と同様の方法で行
った。The results are shown in Table-1. The following procedure was carried out in the same manner as in Example 1.
表−１からキトサンとアスコルビン酸の混合物は酵母の
生育および増殖に対してすぐれた抑制効果を示すことが
わかる。Table 1 shows that the mixture of chitosan and ascorbic acid exhibits an excellent inhibitory effect on the growth and proliferation of yeast.
実施例２
実施例１で使用したｌ、１５％濃度のキトサンルーアス
コルビン酸水溶液を１０％カセイソーダ水溶液でｐＨを
６．０〜６．５に調整した後、０．４５μのフィルター
を用いて滅菌し、次いでオートクレーブ滅菌してミュラ
ーヒントン培地（Ｄｉｆｋｏ Ｌａｂｏｒａｔｏｒｉｅ
ｓ製）で倍々希釈を行って、キトサン濃度の異なる培地
を調整した。この培地に予め３７℃で２４時間で前々培
養。Example 2 After adjusting the pH of the 15% chitosan-ascorbic acid aqueous solution used in Example 1 to 6.0 to 6.5 with a 10% caustic soda aqueous solution, it was sterilized using a 0.45μ filter. , then autoclaved to sterilize Mueller-Hinton medium (Difko Laboratory).
(manufactured by S. Co., Ltd.) to prepare media with different chitosan concentrations. Preliminary culture was performed in this medium at 37°C for 24 hours.
前培養して表−２に示す細菌を植え、３７℃で２４時間
培養を行い最少発育阻止濃度を求めた。結果を表−２に
示す。Precultured bacteria shown in Table 2 were planted, cultured at 37°C for 24 hours, and the minimum inhibitory concentration was determined. The results are shown in Table-2.
比較例３
実施例２で使用したキトサンルーアスコルビン酸水溶液
に代え、キトサン／酢酸水溶液を用いた以外は実施例２
と同様にして最少発育阻止濃度を求めた。結果を表−２
に示す。Comparative Example 3 Example 2 except that chitosan/acetic acid aqueous solution was used in place of the chitosan-ascorbic acid aqueous solution used in Example 2.
The minimum inhibitory concentration was determined in the same manner as above. Table 2 of the results
Shown below.
比較例４
実施例２で使用したキトサンルーアスコルビン酸水溶液
に代えＬ−アスコルビン酸を用いた以外は実施例２と同
様にして最少発育阻止濃度を求めた。Comparative Example 4 The minimum inhibitory concentration was determined in the same manner as in Example 2, except that L-ascorbic acid was used instead of the chitosan-ascorbic acid aqueous solution used in Example 2.
結果を表−２に示す。The results are shown in Table-2.
表−２
実施例３
０．４６％のキトサンルーアスコルビン酸水溶液（キト
サン：Ｌ−アスコルビン酸の重量比＝１．０： Ｌ、３
）にあまえび、赤かい、ねぎとろ、鮪を１分間浸せきし
たのち各々を取り出しトレイパックして５〜１０℃の温
度で保存し一般生菌数と大腸菌の有無を調べた。Table 2 Example 3 0.46% chitosan-ascorbic acid aqueous solution (weight ratio of chitosan:L-ascorbic acid = 1.0: L, 3
) were soaked for 1 minute, then each was taken out, packed in a tray, and stored at a temperature of 5 to 10°C, and the number of viable bacteria and the presence of Escherichia coli were examined.
−殺生菌数は、ＩＭ準寒天培養法による。又、大腸菌は
、ＥＣテスト法による。−殺生菌数は、試料１ｇ当り、
大腸菌は試料０．１ｇ当りにて示す、これらの結果を表
−３に示す。- The number of killed bacteria is determined by the IM semi-agar culture method. In addition, E. coli is tested using the EC test method. - The number of killed bacteria is per 1 g of sample,
E. coli is expressed per 0.1 g of sample, and the results are shown in Table 3.
比較例５
実施例３のキトサン化−アスコルビン酸水溶液での処理
を行わないで、同様の保存を行った。この−殺生菌数、
大腸菌についての結果も、合わせて表−３に示す。Comparative Example 5 The same preservation as in Example 3 was carried out without the treatment with the chitosanated-ascorbic acid aqueous solution. This - the number of viable bacteria,
The results for E. coli are also shown in Table 3.
Claims (2)
ることを特徴とする酵母あるいは細菌の生育及び増殖抑
制剤。(1) A yeast or bacterial growth and proliferation inhibitor characterized by containing chitosan and ascorbic acid as active ingredients.
１：０．９〜３．０である特許請求の範囲第１項記載の
酵母あるいは細菌の生育及び増殖抑制剤。(2) The yeast or bacterial growth and proliferation inhibitor according to claim 1, wherein the ratio of chitosan to ascorbic acid used is 1:0.9 to 3.0 by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18655388A JP2681374B2 (en) | 1988-07-25 | 1988-07-25 | Yeast or bacterial growth and growth inhibitor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18655388A JP2681374B2 (en) | 1988-07-25 | 1988-07-25 | Yeast or bacterial growth and growth inhibitor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0235065A true JPH0235065A (en) | 1990-02-05 |
| JP2681374B2 JP2681374B2 (en) | 1997-11-26 |
Family
ID=16190532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18655388A Expired - Lifetime JP2681374B2 (en) | 1988-07-25 | 1988-07-25 | Yeast or bacterial growth and growth inhibitor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2681374B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6352727B1 (en) | 1998-03-12 | 2002-03-05 | Oji Paper Co., Ltd. | Bactericides |
| JP2002069101A (en) * | 2000-09-01 | 2002-03-08 | Kyowa Technos:Kk | Near neutral aqueous chitosan solution, its dried product, and method of producing them |
| KR100711109B1 (en) * | 2005-05-12 | 2007-04-24 | 김순동 | Preparation of chitosan-ascorbate powder enhanced with antioxidant activity, antimicrobe, and heat and pH staility |
| JP2008285430A (en) * | 2007-05-16 | 2008-11-27 | Yeemey Biotech Co Ltd | Germicidal composition |
-
1988
- 1988-07-25 JP JP18655388A patent/JP2681374B2/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6352727B1 (en) | 1998-03-12 | 2002-03-05 | Oji Paper Co., Ltd. | Bactericides |
| JP2002069101A (en) * | 2000-09-01 | 2002-03-08 | Kyowa Technos:Kk | Near neutral aqueous chitosan solution, its dried product, and method of producing them |
| KR100711109B1 (en) * | 2005-05-12 | 2007-04-24 | 김순동 | Preparation of chitosan-ascorbate powder enhanced with antioxidant activity, antimicrobe, and heat and pH staility |
| JP2008285430A (en) * | 2007-05-16 | 2008-11-27 | Yeemey Biotech Co Ltd | Germicidal composition |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2681374B2 (en) | 1997-11-26 |
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