JPH02231073A - Cultivation of basidiomycetes - Google Patents
Cultivation of basidiomycetesInfo
- Publication number
- JPH02231073A JPH02231073A JP1048620A JP4862089A JPH02231073A JP H02231073 A JPH02231073 A JP H02231073A JP 1048620 A JP1048620 A JP 1048620A JP 4862089 A JP4862089 A JP 4862089A JP H02231073 A JPH02231073 A JP H02231073A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- container
- basidiomycetes
- culture
- inoculated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000221198 Basidiomycota Species 0.000 title claims abstract description 29
- 238000009423 ventilation Methods 0.000 claims abstract description 9
- 239000002609 medium Substances 0.000 claims description 35
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 14
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 10
- 240000000599 Lentinula edodes Species 0.000 abstract description 9
- 238000012258 culturing Methods 0.000 abstract description 7
- 235000001715 Lentinula edodes Nutrition 0.000 abstract description 5
- 238000003892 spreading Methods 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 11
- 229920006395 saturated elastomer Polymers 0.000 description 9
- 238000003756 stirring Methods 0.000 description 6
- 238000001816 cooling Methods 0.000 description 5
- 238000004891 communication Methods 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 240000000731 Fagus sylvatica Species 0.000 description 2
- 235000010099 Fagus sylvatica Nutrition 0.000 description 2
- 240000006499 Flammulina velutipes Species 0.000 description 2
- 235000016640 Flammulina velutipes Nutrition 0.000 description 2
- 244000168667 Pholiota nameko Species 0.000 description 2
- 235000014528 Pholiota nameko Nutrition 0.000 description 2
- 241000219492 Quercus Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000218645 Cedrus Species 0.000 description 1
- 244000077995 Coix lacryma jobi Species 0.000 description 1
- 235000007354 Coix lacryma jobi Nutrition 0.000 description 1
- 240000001080 Grifola frondosa Species 0.000 description 1
- 235000007710 Grifola frondosa Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000190020 Zelkova serrata Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
Description
【発明の詳細な説明】 （１）産業上の利用分野 本願発明は担子菌類の培養方法に関する。[Detailed description of the invention] (1) Industrial application fields The present invention relates to a method for culturing basidiomycetes.
（２）従来の技術
ナメコ、シイタケ、エノキタケ等の担子菌類の培養は、
オガ粉等の木質原料にコメヌカ等の栄養源を配合した培
地を小型容器に移し、オートクレープ等で加熱殺菌後冷
却し、これに担子菌を接種して好適な環境下で培養する
人工培養方法が一般的に行なわれている。(2) Conventional technology Cultivation of basidiomycetes such as nameko, shiitake, and enokitake is as follows:
An artificial culture method in which a medium containing a wood material such as sawdust and a nutrient source such as rice bran is transferred to a small container, heat sterilized using an autoclave, etc., cooled, and basidiomycetes are inoculated into the medium and cultured in a suitable environment. is commonly practiced.
（３）発明が解決しようとする問題点
しかしながら前記従来例において、■培地調製、■容器
充填、■殺菌、■冷却、■種菌の接種、■培養等の各工
程は、各々別々の装置で行なわれており、各装置間の移
送に多大な設備と労力を要していた。(3) Problems to be Solved by the Invention However, in the conventional example described above, each process such as (1) culture medium preparation, (2) container filling, (2) sterilization, (2) cooling, (2) seed inoculation, and (4) culture is performed in separate devices. Therefore, it required a great deal of equipment and labor to transfer between each device.
また場所の移動が多いことはそれだけ雑菌に汚染される
確率も高く、好ましいことではない。Also, the more you move from place to place, the more likely you are to be contaminated with germs, which is not a good thing.
かかる現状に鑑み本願発明者は鋭意研究の結果、密閉容
器に培地を投入し殺菌後、そこに種菌を接種し、酸欠防
止のため空気の供給を行なえば、培地の殺菌から担子菌
の蔓延すなわち菌糸塊の製造までを同一容器内で行なう
ことができ、担子菌培．養の効率化が可能であるという
こと、さらに前記工程を撹拌しながら行なえばより一層
効果的であることを知見し本願発明を完成させた。In view of the current situation, the inventor of the present application has conducted intensive research and found that if a culture medium is placed in a sealed container, sterilized, seed bacteria is inoculated there, and air is supplied to prevent oxygen deficiency, the sterilization of the culture medium and the spread of basidiomycetes can be prevented. In other words, all steps up to the production of mycelial masses can be carried out in the same container, allowing the production of basidiomycete cultures. The present invention was completed based on the findings that it is possible to improve the efficiency of the nurturing process, and that it is even more effective if the above steps are performed while stirring.
すなわち密閉容器に担子菌の培地を投入し、次いで該培
地を加熱殺菌して冷却後、担子菌を接種して通風しつつ
同一容器内で担子菌の培養を行なうことを特徴とする担
子菌の培養方法である。以下本願発明を具体的に説明す
る。That is, a basidiomycete culture medium is introduced into a closed container, and then the medium is heat sterilized and cooled, after which the basidiomycete is inoculated and the basidiomycete is cultured in the same container with ventilation. This is a culture method. The present invention will be specifically explained below.
（４）問題点を解決するための具体的手段本願発明の対
象としている担子菌類としてはナメコ、ヒラタケ、マイ
タケ、エノキタケ、およびシイタケ等が挙げられ、培地
としてはスギ、ナラ、ブナ、クヌギ、ラワン、ケヤキ、
およびカエテ等ｆ）木質原料の粉砕物にフメヌカ、コー
ンプラン、ハトムギヌカ、およびオオムギヌカ等の栄養
源を添加したものを利用することができる。(4) Specific means for solving the problems Basidiomycetes targeted by the present invention include nameko, oyster mushroom, maitake, enokitake, and shiitake, and culture media include cedar, oak, beech, oak, and lauan. , Keyaki,
and kaete, etc. f) A pulverized product of woody raw materials to which a nutrient source such as Japanese bran, corn plan, adlay bran, and barley bran can be added can be used.
このような培地を密閉容器に投入後、１１０〜１３０゜
Ｃの飽和水蒸気で６０〜１２０分間加熱殺菌し冷却後、
担子菌を接種し、通風さらには撹拌しながら担子菌を培
養する。培養には例えば第１図に示すような装置を用い
ることができる。After putting such a culture medium into a closed container, heat sterilize it with saturated steam at 110 to 130 °C for 60 to 120 minutes, and after cooling,
Basidiomycetes are inoculated and cultured with ventilation and stirring. For example, an apparatus as shown in FIG. 1 can be used for culturing.
第１図において１は水平円筒型の容器で、左右の側板２
，３の中心部には支持軸４．５が突設されており、また
周胴部６には培地の投入口７が配置されていて、該投入
口７は開閉自在のカバー８にて密閉される。そして容器
１内には、その軸と平行状に多孔板９が敷設されており
、培地床を形成する。In Figure 1, 1 is a horizontal cylindrical container, with left and right side plates 2
, 3 has a support shaft 4.5 protruding from the center thereof, and a culture medium inlet 7 is arranged in the circumferential body 6, and the inlet 7 is sealed with a cover 8 that can be opened and closed. . A perforated plate 9 is placed inside the container 1 parallel to its axis to form a culture medium bed.
支持軸４，５は左右の軸受１ｏで軸支され一方の支持軸
４は中空状に形成されており、その内部に空気あるいは
飽和水蒸気が流通する連通管１１が挿通されて、一端は
前記多孔板９と容器の周胴部６で形成されるカマボコ状
ｆ７）室２７Ｅ臨み、他端は外部に開口している。連通
管１１は支持軸４の中心部において、軸受１２を介して
軸支されている。The support shafts 4 and 5 are supported by left and right bearings 1o, and one of the support shafts 4 is formed in a hollow shape, into which a communication pipe 11 through which air or saturated steam flows is inserted, and one end is connected to the porous hole. A semicylindrical chamber 27E formed by the plate 9 and the circumferential body 6 of the container faces the chamber 27E, and the other end is open to the outside. The communication pipe 11 is pivotally supported at the center of the support shaft 4 via a bearing 12 .
そして連通管１１には送風機１３がロータリージ３イン
｝１４を介して連通されており、送風機１３とロータリ
ージョイント１４０間には除菌フィルター１５、送風空
気を加湿する調湿器１６が介装されている。A blower 13 is connected to the communication pipe 11 via a rotary joint 14, and a sterilization filter 15 and a humidifier 16 for humidifying the blown air are interposed between the blower 13 and the rotary joint 140. ing.
またロータリージョイント１４と調湿器１６の間の接続
ラインにはパルブ１７を介して飽和水蒸気源１８が接続
されている。Further, a saturated steam source 18 is connected to the connection line between the rotary joint 14 and the humidifier 16 via a valve 17.
１９は排気口で、通風された空気の排気作用をするが、
そこにはバルブ２ｏを介しテ除菌フィルター２１を設け
ることが好ましい。19 is an exhaust port, which functions to exhaust the ventilated air.
It is preferable to provide a sterilization filter 21 there via a valve 2o.
２２は回転ハンドルで、容器１を１８０度回転させ菌糸
が蔓延した培地を外部へ投入口７を介して排出させる作
用をする。Reference numeral 22 denotes a rotary handle that rotates the container 1 by 180 degrees and discharges the medium infested with mycelia to the outside through the input port 7.
この装置を用いて担子菌を培養する場合、まず培地の投
入口７より培地を投入し、次いでバルブ１７を開き飽和
水蒸気を通気させ容器１内を高温加圧状態に保持し殺菌
を行なう。なおこのときバルブ２０．２３は閉にしてお
く。When culturing basidiomycetes using this device, a medium is first introduced through the medium inlet 7, and then the valve 17 is opened to allow saturated steam to pass through and the inside of the container 1 is maintained at a high temperature and pressurized state for sterilization. At this time, the valves 20 and 23 are kept closed.
次にバルブ１７を閉に、バルプ２０．２３を開にして容
器１へ送風機１３により冷却空気を送気して培地の冷却
をするが、調湿機１６は停止しておく。Next, the valve 17 is closed, the valves 20.23 are opened, and the blower 13 blows cooling air into the container 1 to cool the culture medium, but the humidity controller 16 is stopped.
培地冷却後投入口７より担子菌を接種後、高湿空気を通
風しつつ担子菌の培養を該菌が培地に蔓延するまでおよ
そ７〜１０日間程度行なう。After cooling the medium, the basidiomycetes are inoculated through the inlet 7, and the basidiomycetes are cultured for about 7 to 10 days while ventilation with high humidity air is carried out until the bacteria spread in the medium.
次の工程である子実体の育成は容器１を１８０度回転さ
せ投入口７から培地を排出し、通常の方法で培養すれば
よく、例えばベッド栽培、小型容器栽培等を挙げること
ができる。The next step, the cultivation of fruiting bodies, can be carried out by rotating the container 1 180 degrees, discharging the medium from the inlet 7, and culturing in a conventional manner, such as bed cultivation or small container cultivation.
担子菌培養中の通風量であるが、菌の種類、培地の粒度
、培地の量等で異なるが、要は担子菌の生育に必要な酸
素が供給され、かつ菌の培養にともない発生する炭酸ガ
スを培地中から飛散させればよいのであって、例えばシ
イタケ菌を２５Ｌ程度の培地で培養する場合で、ＩＯＬ
／ｍｉｎ程度の通気量で十分である。The amount of ventilation during basidiomycete culture varies depending on the type of bacteria, the particle size of the medium, the amount of medium, etc., but the key is to supply the oxygen necessary for the growth of basidiomycetes, and to maintain the carbon dioxide generated as the bacterium grows. It is sufficient to disperse the gas from the medium. For example, when culturing Shiitake mushrooms in a medium of about 25 L, IOL
A ventilation amount of about 1/min is sufficient.
容器１への種菌の接種手段であるが、第５図に示す如く
真空冷却機３ｏおよび種菌供給装置３１を容器１に連通
設置し、真空冷却機３ｏで吸引冷却して該冷却＠３ｏを
停止後、種菌供給装置３１のバルブ３２を開き、除菌フ
ィルター３３を介して吸引される空気とともに種菌を接
種してもよい。As for the means for inoculating the seed bacteria into the container 1, as shown in FIG. Thereafter, the valve 32 of the seed culture supply device 31 may be opened, and the seed bacteria may be inoculated together with the air sucked through the sterilization filter 33.
次に第２図に容器１を回転すなわち培地を撹拌しながら
、殺菌および通風培養する例について説明する。Next, an example in which sterilization and aeration culture are performed while rotating the container 1, that is, stirring the culture medium, will be described with reference to FIG.
本実施例については駆動軸２４を中空状に形成し、側板
２，３の中心部を貫通させて設けてあり、容器１の内部
に臨む部分には多数の小孔２５が穿孔されている。そし
て駆動軸２４の一端には送風機１３、除菌フィルター１
５、調湿器１６、および飽和水蒸気源１８等が第１図と
同様に連通されており、前記小孔２５より空気あるいは
水蒸気が噴出されるよう構成されている。他端には駆動
輪２６が設けられ、モーターにより駆動軸２４を介して
容器１は回転自在に構成されている。In this embodiment, the drive shaft 24 is formed into a hollow shape and is provided so as to pass through the center of the side plates 2 and 3, and a large number of small holes 25 are bored in the portion facing the inside of the container 1. At one end of the drive shaft 24, a blower 13 and a sterilizing filter 1 are provided.
5, a humidity controller 16, a saturated steam source 18, etc. are connected in the same way as in FIG. A drive wheel 26 is provided at the other end, and the container 1 is configured to be freely rotatable by a motor via a drive shaft 24.
２８は容器１の周胴部６の内壁面に設置された帯状の固
定翼で、撹拌効果向上のために設けられている。Reference numeral 28 denotes a band-shaped fixed blade installed on the inner wall surface of the circumferential body 6 of the container 1, and is provided to improve the stirring effect.
この装置を用いて担子菌を培養する場合、まず培地の投
入口７より培地を投入し、次いでバルブ１７を開き飽和
水蒸気を通気させ容器１内を高温加圧状態に保持し、該
容器１を回転させて培地を撹拌しつつ殺菌を行なう。な
おこのときパルブ２０．２３は閉にしておく。When culturing basidiomycetes using this device, first introduce a medium from the medium inlet 7, then open the valve 17 to vent saturated steam and maintain the inside of the container 1 in a high temperature and pressurized state. Sterilize the medium while stirring it by rotating. At this time, valves 20 and 23 are kept closed.
次にバルブ１７を閉に、バルブ２０．２３を開にして容
器１へ送風機１３により冷却空気を送気して培地の冷却
をする。このとぎも容器１は回転させておくが、調湿機
１６は停止しておく。Next, the valve 17 is closed, the valves 20.23 are opened, and cooling air is blown into the container 1 by the blower 13 to cool the culture medium. The togimo container 1 is kept rotating, but the humidity controller 16 is stopped.
培地冷却後投入口７より担子菌を投入し、容器１を回転
させながら培地と菌の混合を行ない、高湿空気を通風し
つつ担子菌の培養な該菌が培地に蔓延するまでおよそ７
〜１０日間程度行なう。以下は第１図の実施例と同じで
ある。なお排気口１９と除菌フィルター２１は着脱自在
の継ぎ手で接続し、容器１の回転時は外しておく。After the medium has cooled, basidiomycetes are introduced through the inlet 7, and the medium and bacteria are mixed while rotating the container 1, and the basidiomycetes are cultured while ventilating high humidity air until the basidiomycetes are spread in the medium.
Do this for about 10 days. The following is the same as the embodiment shown in FIG. The exhaust port 19 and the sterilizing filter 21 are connected by a removable joint, and are removed when the container 1 is rotated.
本実施例の場合容器１を回転させながら培地の殺菌ある
いは菌の培養をするため、菌も培地に均等に分散され菌
糸の繁殖も良好で、殺菌および通風の効果も一層増大す
る。In this embodiment, since the medium is sterilized or the bacteria are cultured while the container 1 is rotated, the bacteria are evenly dispersed in the medium, the hyphae propagate well, and the effects of sterilization and ventilation are further enhanced.
そして次に第４図に示す実施例は、培地の撹拌手段とし
て容器１の中に回転式撹拌機２９を設けた例である。そ
の他密閉容器としてはリボン型混合機、Ｖ型混合機等の
回転式混合機を利用することができる。Next, the embodiment shown in FIG. 4 is an example in which a rotary stirrer 29 is provided in the container 1 as a means for stirring the medium. Other airtight containers that can be used include rotary mixers such as ribbon mixers and V-type mixers.
また前記第１図の実施例において、容器１を回転しなが
ら培養してもよいことは言うまでもな（）。Furthermore, in the embodiment shown in FIG. 1, it goes without saying that the culture may be carried out while rotating the container 1 ().
（５）発明の効果
本願発明は密閉容器内で担子菌を培養するため、培地の
殺菌から菌の蔓延までを同一容器内で行なうことができ
、製造設備が簡略化され、自動化し無人運転も可能であ
る。(5) Effects of the invention Since the present invention cultivates basidiomycetes in a closed container, everything from sterilizing the culture medium to spreading the bacteria can be carried out in the same container, simplifying the manufacturing equipment and allowing automation and unmanned operation. It is possible.
さらに特に雑菌に汚染され易い初期培養を密閉下で行う
ため、無菌操作が簡単に行なえる。Furthermore, since the initial culture, which is particularly prone to contamination with germs, is carried out under closed conditions, sterile operation can be easily performed.
実施例１
ブナオガ粉にコメヌカを１０％（Ｗ／Ｗ）配合して水分
６０％（Ｗ／Ｗ）になるよう加水したものを培地とし、
外径４Ｑｃｍｓ長さ６０ｃｍの容器を備えた第１図に示
す装置に該培地を２５Ｌ供給した。これを圧力１ｋｇ／
ｃＪＧの飽和水蒸気で１．５時間加熱殺菌し、次いで無
菌空気を容器内に２ ５ Ｌ／ｗｉｎ程度送風しておよ
そ５時間かけて室温近辺まで冷却した。Example 1 A medium was prepared by blending rice bran at 10% (W/W) with beech sawdust powder and adding water to make the water content 60% (W/W).
25 L of the medium was supplied to the apparatus shown in FIG. 1 equipped with a container having an outer diameter of 4 Qcm and a length of 60 cm. Pressure 1kg/
The container was heat sterilized with cJG saturated steam for 1.5 hours, and then sterile air was blown into the container at a rate of about 25 L/win to cool it down to around room temperature over about 5 hours.
そして次に雑菌による汚染防止のため無菌空気を送風し
たまま、シイタケ菌Ｗ４ （森産業株式会社）を接種し
た後、湿度９５％で室温の高湿空気を５Ｌ／ｗｉｎ程度
連続的に送風して、１０日間培地を培養したところ培地
中に菌糸が蔓延した。Next, while sterile air was being blown to prevent contamination by various bacteria, Shiitake fungus W4 (Mori Sangyo Co., Ltd.) was inoculated, and then high-humidity air at room temperature with a humidity of 95% was continuously blown at a rate of about 5 L/win. When the medium was cultured for 10 days, hyphae were spread in the medium.
そして容器を反転させて培地を回収し、ベッド栽培用の
菌床とした。Then, the container was turned over and the medium was collected, which was used as a fungal bed for bed cultivation.
実施例２
ブナオガ粉にコメヌカを１０％（Ｗ／Ｗ）配合して水分
６０％（Ｗ／Ｗ）になるよう加水したものを培地とし、
外径４０ｃｍ，長さ６０ｃｍの容器を備えた第２図に示
す装置に該培地を２５Ｌ供給した。これを圧力１ｋｇ／
ｅＪＧの飽和水蒸気で１時間、容器を２ ｒｐｍ回転さ
せながら加熱殺菌し、次いで容器を回転させたまま無菌
空気を容器内に２５Ｌ／ｗｉｎ程度送風しておよそ３時
間かけて室温近辺まで冷却した。Example 2 A medium was prepared by adding 10% (W/W) of rice bran to Bunaoga flour and adding water to the mixture to give a moisture content of 60% (W/W).
25 L of the medium was supplied to the apparatus shown in FIG. 2, which was equipped with a container having an outer diameter of 40 cm and a length of 60 cm. Pressure 1kg/
The container was heat sterilized with eJG's saturated steam for 1 hour while rotating at 2 rpm, and then sterile air was blown into the container at a rate of about 25 L/win while the container was being rotated, and the container was cooled to around room temperature over about 3 hours.
そして次に前記実施例と同様に無菌空気を送風したまま
、シイタケ菌Ｗ４ （森産業株式会社）を接種し容器を
回転させて培地にシイタケ菌を均等に分散させた後、湿
度９５％で室温の高湿空気を５Ｌ／ｗｉｎ程度連続的に
送風して、７日間培地を培養したところ培地中に菌糸が
蔓延した。なお培養中に容器を１日１度数回転させて培
地を撹拌した。そして回収した培地を前記実施例１と同
様に、ベッド栽培の菌床とした。Then, as in the previous example, while blowing sterile air, inoculate the shiitake fungus W4 (Mori Sangyo Co., Ltd.), rotate the container to evenly disperse the shiitake fungus in the medium, and then leave it at room temperature at 95% humidity. When the medium was cultured for 7 days by continuously blowing high-humidity air at a rate of about 5 L/win, hyphae spread in the medium. During the culture, the container was rotated once a day to agitate the medium. The recovered medium was then used as a fungal bed for bed cultivation in the same manner as in Example 1 above.
第１図は担子菌の培養装置の正面断面図、第２図は他の
実施例図、第３図は第２図のＡ部拡大図、第４図は他の
実施例図、第５図は種菌供給手段の他の実施例図をそれ
ぞれ示す。
なお図面において１は容器、７は投入口、９は多孔板、
１１は連通管、１３は送風機、１５は除菌フィルター
１６は調湿器、１８は飽和水蒸気源をそれぞれ示す。Figure 1 is a front sectional view of a basidiomycete culture device, Figure 2 is another example, Figure 3 is an enlarged view of part A in Figure 2, Figure 4 is another example, and Figure 5. 2A and 2B respectively show diagrams of other embodiments of the seed supply means. In the drawings, 1 is a container, 7 is an inlet, 9 is a perforated plate,
11 is a communication pipe, 13 is a blower, and 15 is a sterilization filter.
16 is a humidifier, and 18 is a saturated steam source.
Claims (1)
殺菌して冷却後、担子菌を接種して通風しつつ同一容器
内で担子菌の培養を行なうことを特徴とする担子菌の培
養方法。Cultivation of basidiomycetes, characterized in that a culture medium of basidiomycetes is put into a closed container, then the medium is heat sterilized, cooled, inoculated with basidiomycetes, and basidiomycetes are cultured in the same container with ventilation. Method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1048620A JPH02231073A (en) | 1989-03-02 | 1989-03-02 | Cultivation of basidiomycetes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1048620A JPH02231073A (en) | 1989-03-02 | 1989-03-02 | Cultivation of basidiomycetes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02231073A true JPH02231073A (en) | 1990-09-13 |
Family
ID=12808451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1048620A Pending JPH02231073A (en) | 1989-03-02 | 1989-03-02 | Cultivation of basidiomycetes |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02231073A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013107026A (en) * | 2011-11-18 | 2013-06-06 | Aichi Electric Co Ltd | Mixing apparatus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS513963A (en) * | 1974-07-08 | 1976-01-13 | Choshi Shoyu Kk | Matsutakekinno ekitaibaiyoho |
| JPS5266679A (en) * | 1975-11-26 | 1977-06-02 | Kureha Chem Ind Co Ltd | Method and apparatus for cultivating basidiomycetes |
| JPS52148651A (en) * | 1976-06-02 | 1977-12-10 | Hayashikane Sangyo | Method of making mushroom spawn food |
| JPS6137032A (en) * | 1984-07-31 | 1986-02-21 | 株式会社 塚越食菌研究所 | Culture method and container of mushroom |
-
1989
- 1989-03-02 JP JP1048620A patent/JPH02231073A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS513963A (en) * | 1974-07-08 | 1976-01-13 | Choshi Shoyu Kk | Matsutakekinno ekitaibaiyoho |
| JPS5266679A (en) * | 1975-11-26 | 1977-06-02 | Kureha Chem Ind Co Ltd | Method and apparatus for cultivating basidiomycetes |
| JPS52148651A (en) * | 1976-06-02 | 1977-12-10 | Hayashikane Sangyo | Method of making mushroom spawn food |
| JPS6137032A (en) * | 1984-07-31 | 1986-02-21 | 株式会社 塚越食菌研究所 | Culture method and container of mushroom |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013107026A (en) * | 2011-11-18 | 2013-06-06 | Aichi Electric Co Ltd | Mixing apparatus |
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