JPH02231023A - Raising seedling of genus pinelliae or genus angelic plant - Google Patents
Raising seedling of genus pinelliae or genus angelic plantInfo
- Publication number
- JPH02231023A JPH02231023A JP1053215A JP5321589A JPH02231023A JP H02231023 A JPH02231023 A JP H02231023A JP 1053215 A JP1053215 A JP 1053215A JP 5321589 A JP5321589 A JP 5321589A JP H02231023 A JPH02231023 A JP H02231023A
- Authority
- JP
- Japan
- Prior art keywords
- callus
- genus
- medium
- plant
- pinelliae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- UIERETOOQGIECD-ARJAWSKDSA-N angelic acid group Chemical group C(\C(\C)=C/C)(=O)O UIERETOOQGIECD-ARJAWSKDSA-N 0.000 title description 2
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 58
- 241000196324 Embryophyta Species 0.000 claims abstract description 38
- 229930192334 Auxin Natural products 0.000 claims abstract description 15
- 239000002363 auxin Substances 0.000 claims abstract description 15
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004062 cytokinin Substances 0.000 claims abstract description 13
- 235000001287 Guettarda speciosa Nutrition 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 14
- 230000006698 induction Effects 0.000 claims description 11
- 230000008929 regeneration Effects 0.000 claims description 10
- 238000011069 regeneration method Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 244000061520 Angelica archangelica Species 0.000 claims 1
- 241000125175 Angelica Species 0.000 abstract description 12
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 abstract description 6
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 abstract description 6
- 229960001669 kinetin Drugs 0.000 abstract description 6
- 241000544270 Angelica acutiloba Species 0.000 abstract 1
- 241001522232 Pinellia ternata Species 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 11
- 241000411851 herbal medicine Species 0.000 description 7
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 7
- 229940079593 drug Drugs 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical group C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000237858 Gastropoda Species 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- IGMNYECMUMZDDF-UHFFFAOYSA-N homogentisic acid Chemical compound OC(=O)CC1=CC(O)=CC=C1O IGMNYECMUMZDDF-UHFFFAOYSA-N 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- 235000003840 Amygdalus nana Nutrition 0.000 description 1
- 244000263228 Angelica sp Species 0.000 description 1
- 235000008490 Angelica sp Nutrition 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000004356 Hysteria Diseases 0.000 description 1
- 206010027304 Menopausal symptoms Diseases 0.000 description 1
- 208000009233 Morning Sickness Diseases 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 206010033546 Pallor Diseases 0.000 description 1
- 235000011432 Prunus Nutrition 0.000 description 1
- 241000220299 Prunus Species 0.000 description 1
- 235000011158 Prunus mume Nutrition 0.000 description 1
- 244000018795 Prunus mume Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 208000034850 Vomiting in pregnancy Diseases 0.000 description 1
- 239000010175 Yi-Gan San Substances 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
- 230000003460 anti-nuclear Effects 0.000 description 1
- FXNFHKRTJBSTCS-UHFFFAOYSA-N baicalein Chemical compound C=1C(=O)C=2C(O)=C(O)C(O)=CC=2OC=1C1=CC=CC=C1 FXNFHKRTJBSTCS-UHFFFAOYSA-N 0.000 description 1
- 229940076810 beta sitosterol Drugs 0.000 description 1
- LGJMUZUPVCAVPU-UHFFFAOYSA-N beta-Sitostanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 LGJMUZUPVCAVPU-UHFFFAOYSA-N 0.000 description 1
- NJKOMDUNNDKEAI-UHFFFAOYSA-N beta-sitosterol Natural products CCC(CCC(C)C1CCC2(C)C3CC=C4CC(O)CCC4C3CCC12C)C(C)C NJKOMDUNNDKEAI-UHFFFAOYSA-N 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000000812 cholinergic antagonist Substances 0.000 description 1
- 230000035601 cold sensitivity Effects 0.000 description 1
- 208000012839 conversion disease Diseases 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000551 menstrual abnormality Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 235000014774 prunus Nutrition 0.000 description 1
- 239000008651 saiko-keishi-to Substances 0.000 description 1
- 239000008535 sairei-to Substances 0.000 description 1
- 239000008485 shosaiko-to Substances 0.000 description 1
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 description 1
- 229950005143 sitosterol Drugs 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は．漢方薬として利用されるカラスビシャク，オ
オハシゲなどのＰｉｎｅｌｌｉａｅ属植物．およびトウ
キ，シシウドなどのＡｎｇｅｌｉｃａ属植物の効果的な
育苗方法に関する。[Detailed Description of the Invention] (Industrial Application Field) The present invention... Plants of the genus Pinelliae, such as crow's snail and snail, which are used as Chinese medicine. The present invention also relates to an effective method for raising seedlings of plants belonging to the genus Angelica, such as Angelica sp.
（従来の技術）
属に属する植物であり，日本，中国本土，朝鮮半島など
に自生する多年草である。これらの植物は．地下茎とし
て直径１〜３ ｃｍの球茎をつける。この球茎の外皮を
除去して乾燥したものはハンゲ（半夏）と呼ばれ，古く
から．漢方薬に利用されている。(Prior art) It is a perennial plant that belongs to the genus Prunus genus and grows naturally in Japan, mainland China, the Korean Peninsula, etc. These plants are. A corm with a diameter of 1 to 3 cm is produced as an underground rhizome. The dried product after removing the outer skin of this corm is called hange (half summer) and has been used since ancient times. Used in Chinese medicine.
この半夏は，漢方では，悪心嘔吐．つわり．胃部停水，
心痛．咳徹．儂中雷鳴．ａｍ痛などに対する処方の生薬
として利用されている。半夏の有効成分は明らかにされ
てはいないが，ホモゲンチジン酸，β−シトステロール
，トリテルペン順，各種配糖体．グルコン酸誘導体など
が知られている。In Chinese medicine, this half-summer is associated with nausea and vomiting. Morning sickness. Gastric hydration,
Heartache. Cough. My middle thunder. It is used as a herbal medicine for prescriptions such as aching pain. The active ingredients of Hanka are not known, but they include homogentisic acid, β-sitosterol, triterpenes, and various glycosides. Gluconic acid derivatives are known.
半夏を用いた漢方薬としては，半夏厚朴湯，半夏瀉心湯
，半夏白求麻蘂，茨苓飲加半夏，小半夏加茨苓湯．半夏
敗料，半夏楡湯，大柴胡湯．小柴胡湯，紫陥湯，柴胡加
竜骨物密湯，柴胡桂枝湯，柴苓湯などがある。Chinese herbal medicines using Hanka include Hanka Kobokuto, Hankashashinto, Hanka Shiragu Maya, Ibarei Drinka Hanka, and Shohanka Bareito. Han-ka-rei, Han-ka-el-to, O-saiko-to. These include Shosaiko-to, Shiwa-to, Saikokaryu-kotsumitsutou, Saiko-keishi-to, and Sairei-to.
のＡｎｇｅｌｉｃａ属植物も，その根部を乾燥し，漢方
薬として利用されている。特にトウキの根部を湯通しし
て乾燥したもの（当帰）は．婦人病薬として利用され，
あるいは各種漢方薬に配合されている。The roots of plants belonging to the genus Angelica are also dried and used as Chinese medicine. Especially those made by blanching and drying the roots of the Japanese Angelica (dōki). Used as a medicine for women's diseases,
It is also included in various Chinese herbal medicines.
例えば，貧血，諸庸瘍．冷え症，月経異常，不妊症，腹
部の冷痛，更年期障害，ヒステリー，頭痛などの生薬と
して用いられている。婦人病薬以外の漢方薬としては，
鎮痛鎮痙薬として当帰建中湯，当帰四逆湯．当帰荀薬敗
などに配合され；精神神経薬として逍遥散，加味逍遥敗
，抑肝散，帰牌湯などに配合され；鎮核去療薬として蘇
子降気湯，滋陰至宝湯，清肺湯などに配合され；健胃消
化薬として正積散に配合され；あるいは瀉千薬とじて潤
腸湯，通導敗などに配合される。For example, anemia, various ulcers. It is used as a herbal medicine for cold sensitivity, menstrual abnormalities, infertility, abdominal cold pain, menopausal symptoms, hysteria, headaches, etc. Other than gynecological medicines, Chinese herbal medicines include:
Tokikenchuto and Tokishigyakuto are used as analgesic and antispasmodic drugs. It is used in medicines such as Dangkishuan medicine; it is used as psychoneurotic drugs in Shoyosan, Kamishoyobei, Yokukansan, Kipaito, etc.; it is used as antinuclear castration medicines in Sozifurikito, Shiinshihoto, and Seihinshihoto. It is added to rento, etc.; it is added to seisekisan as a digestive aid for the stomach; or it is added to junchoto, toudou, etc. as a shasenyaku.
これらのＰｉｎｅｌｌｉａｅ属およびＡｎｇｅｌ ｉｃ
ａ属植物の植物体のうち生薬として使用されるのは．い
ずれも球茎あるいは根部といった地下に存在する栄養貯
留部分である。例えば，半夏の母植物であるカラスビシ
ャク（Ｐｉｎｅｌｌｉａｅ属植物）の栽培は比較的容易
であるが，球茎は，１草あたり．年間に通常３〜６個の
割合でしか得られない。植物をほり起こして球茎部を採
取すると該植物体は枯死するため．毎年球茎を採取する
には，球茎を保存して，これを播種しなければならない
。そのため，球茎を多量に得ることができない。トウキ
（Ａｎｇｅｌｉｃａ属）についても．薬用部分である根
部が生育するには３〜４年を要し，これを収穫すると植
物が枯死する。そのため，毎年収穫を行なうには発芽育
成用の畑ふよび収穫用の畑をわけて栽培を行なう必要が
あり，多大な労力と広大な耕地とを必要とする。さらに
，これら植物が栽培され得る季節および地域には制限が
あるうえ，薬用部分である球茎部や根邪に含有される有
効成分の比率は．植物が栽培される地域や気候により異
なり，品質が一定しないという欠点がある。所定の品質
の生薬を多量に得るために，これら植物の幼苗を短期間
に効果的に生産する方法が望まれている。These Pinelliae genus and Angelic
Among plants belonging to the genus A, which are used as herbal medicines. Both are nutrient storage parts that exist underground, such as corms or roots. For example, it is relatively easy to cultivate the mid-summer mother plant, a plant of the genus Pinelliae, but the number of corms per plant is small. Usually only 3 to 6 pieces are obtained per year. If you dig up the plant and collect the corm, the plant will wither. To collect corms each year, you must save them and sow them. Therefore, it is not possible to obtain large quantities of corms. Regarding Angelica (genus Angelica). It takes three to four years for the medicinal part, the root, to grow, and when it is harvested, the plant dies. Therefore, in order to harvest each year, it is necessary to cultivate separate fields for germination and cultivation and for harvesting, which requires a great deal of labor and vast amounts of cultivated land. Furthermore, there are restrictions on the seasons and regions in which these plants can be cultivated, and the ratio of active ingredients contained in the medicinal parts of the corms and roots. The drawback is that the quality is inconsistent, depending on the region and climate where the plant is grown. In order to obtain a large quantity of crude drugs of a given quality, a method for effectively producing seedlings of these plants in a short period of time is desired.
（発明が解決しようとする課題）
本発明は上記従来の問題点を解決するものであり，その
目的とするところは，気候，土壌，季節などに関係なく
，短時間でｐｉｎ６１１ｉａｅ属またはＡｎｇｅｌｉｃ
ａ属植物の幼苗を効果的に得る方法を提供することにあ
る。(Problems to be Solved by the Invention) The present invention solves the above-mentioned conventional problems, and its purpose is to quickly eliminate the genus pin611iae or Angelicus, regardless of the climate, soil, season, etc.
An object of the present invention is to provide a method for effectively obtaining seedlings of plants of the genus A.
（課題を解決するための手段）
の組織をオーキシン類およびサイトヵイニン類を含有す
るカルス誘導用培地を用いて培養しカルスを誘導する工
程，該カルスをカルス増殖用培地で増殖させる工程，お
よび該カルスを再分化用培地で光を照射して分化させ幼
苗とする工程を包含し，そのことにより上記目的が達成
される。(Means for Solving the Problems) A step of inducing callus by culturing the tissue of the above using a callus induction medium containing auxins and cytokinins, a step of growing the callus in a callus growth medium, and a step of growing the callus in a callus growth medium. The above-mentioned objective is achieved by irradiating light in a redifferentiation medium to differentiate into seedlings.
本発明方法によれば，まず， Ｐｉｎｅｌｌｉａｅ属ま
たはＡｎｇｅｌｉｃａ属植物の根，茎，葉などの生組織
の一部を切りとり，これを用いてカルス誘導用培地で培
養しカルスの誘導を行う。使用される植物組織はどの部
分でもよいが効率的にカルスを誘導するには，例えば，
Ｐｉｎｅｌｌｉａｅ属植物においては球茎部，Ａｎｇ
ｅ ｌ ｉｃａ属植物においては伸長する芽の先端部や
若い茎部が好適である。カルス誘導用培地としては，植
物組織培養に通常用いられるムラシゲースクーグの培地
，ホワイトの培地，リンスマイヤー−スクーグの培地，
ガウスレットの培地．ヘラーの培地，およびこれらの改
変培地などが用いられ，これにカルスの誘導を促進させ
るために才一キシン類およびサイトカイニン類が添加さ
れる。オーキシン類には例えば，２・４−ジクロロフエ
ノキシ酢酸（２・４Ｄ），インドール酢酸（ＩＡＡ），
ナフタレン酢酸（ＮＡＡ）がある。サイトカイニン類に
は，例えば，カイネチン，ペンジルアデニンがある。According to the method of the present invention, first, a part of the living tissue such as the roots, stems, and leaves of a plant of the genus Pinelliae or Angelica is cut off, and this is used to culture in a callus induction medium to induce callus. Any part of the plant tissue can be used, but in order to efficiently induce callus, for example,
In plants of the genus Pinelliae, the corm, Ang
For plants of the genus Elica, the tips of elongating buds and young stems are suitable. Callus induction media include Murashigeskoog's medium, White's medium, Linsmeyer-Skoog's medium, which are commonly used for plant tissue culture.
Gauslet's medium. Heller's medium and modified media thereof are used, and to this medium, cytoxins and cytokinins are added to promote callus induction. Examples of auxins include 2,4-dichlorophenoxyacetic acid (2,4D), indoleacetic acid (IAA),
There is naphthalene acetic acid (NAA). Examples of cytokinins include kinetin and penzyladenine.
オーキシン類は，通常．０．０１〜１０ｐｐｍ ，好ま
し＜ハｏ．１〜１ ｐｐｍの割合で，サイトカイニン類
は，０．’０１〜ｌＯｐｐｍ ，好ましくは０．１ 〜
ｌｐｐｍの割合で培地中に含有される。これら植物ホル
モン（才一キシン類やサイトカイニン類）の量が過少で
あるとカルスが誘導されに＜＜，過剰であると直接，不
定根が分化したり．成長阻害等が認められる。Auxins are usually. 0.01 to 10 ppm, preferably <hao. At a rate of 1 to 1 ppm, cytokinins are present at a rate of 0. '01~lOppm, preferably 0.1~
It is contained in the medium at a ratio of lppm. If the amount of these plant hormones (cytokinins and cytokinins) is too low, callus will be induced, and if it is in excess, adventitious roots will directly differentiate. Growth inhibition etc. are observed.
カルス誘導培地で１５〜３５℃にて，通常暗所で，培養
を行うと２０〜５０日後には組織切断面にカルスが形成
される。必要に応じてこのカルスを適当な固体培地に移
して適当な量にまで増殖させる。When culturing is carried out in a callus induction medium at 15 to 35° C., usually in the dark, callus will be formed on the cut surface of the tissue after 20 to 50 days. If necessary, this callus is transferred to an appropriate solid medium and grown to an appropriate amount.
カルスを次に．カルス増殖用培地に移植して培養を行う
。カルス増殖用培地としてはムラシゲースクーグの培地
など組織培養に通常用いられる培地（寒天培地などの固
体培地または液体培地）が利用されうる。上記カルス誘
導用培地と同様の培地を用いてもよい。オーキシン類の
濃度は１０ｐｐｍ以下とすることが好ましい。オーキシ
ン類の濃度が高すぎると安定したカルスの増殖が得られ
ず，以下の再分化工程で，再分化しにくくなる。植物ホ
ルモンが全く含有されていなくてもよい。培養は１５〜
３０℃．好ま′シ＜は２０〜２５℃で通常暗所にて行わ
れる。増殖したカルスのうち増殖の速いカルスを選抜す
れば．生長の速い植物の幼苗が得られる。Next is the callus. Transplant to callus growth medium and culture. As the callus growth medium, a medium (a solid medium such as an agar medium or a liquid medium) commonly used for tissue culture, such as Murashige-Skoog's medium, can be used. A medium similar to the above callus induction medium may be used. The concentration of auxins is preferably 10 ppm or less. If the concentration of auxins is too high, stable callus growth cannot be obtained, and regeneration becomes difficult in the following regeneration step. It may not contain any plant hormones at all. Culture is from 15 to
30℃. Preparation is preferably carried out at 20 to 25°C, usually in a dark place. If calli that proliferates quickly are selected from among the proliferated calli. Young seedlings of fast-growing plants can be obtained.
このようにして増殖したカルスを再分化用培地に移して
カルスを再分化させる。再分化用培地としては，植物組
織培養に通常用いられる培地にカイネチン．ベンジルア
デニンなどのサイトカイニン類が添加された，もしくは
無添加の，通常の植物カルス再分化用培地（固体培地）
が利用されうる。再分化用培地にオーキシン類が含有さ
れる場合には，その濃度はカルス増殖用培地よりも低く
設定し，例えば２．４−Ｄでは０．５ｐｐｍ以下，好ま
しくは０．１ｐｐｍ以下とされる。オーキシン類が全く
含有されていなくてもよい。このような再分化用培地に
カルスを置床し，１０〜３０℃．好ましくは１５〜２０
℃の温度条件下で， １０００〜１００００ルクスの光
を照射して培養を行う。光量が過少であると再分化が遅
れ，または再分化が行われず．過剰であると生長阻害が
認められる。このような条件下で培養を行うとカルスは
緑化し，葉緑素を有する組織が形成され出芽が起こる。The calli grown in this way are transferred to a regeneration medium to regenerate the callus. As a medium for redifferentiation, kinetin is added to the medium normally used for plant tissue culture. Ordinary plant callus redifferentiation medium (solid medium) with or without the addition of cytokinins such as benzyladenine
can be used. When the regeneration medium contains auxins, the concentration thereof is set lower than that of the callus growth medium, for example, for 2.4-D, it is 0.5 ppm or less, preferably 0.1 ppm or less. It is not necessary to contain any auxins at all. Callus was placed on such a regeneration medium and heated at 10 to 30°C. Preferably 15-20
Culture is performed under temperature conditions of 1000 to 10000 lux under temperature conditions of 1000 to 10000 lux. If the amount of light is too low, redifferentiation will be delayed or will not occur. If it is in excess, growth inhibition is observed. When cultured under these conditions, the callus turns green, a tissue containing chlorophyll is formed, and budding occurs.
さらに培養を続けると該組織から母植物と同様の葉茎を
伸ばし，次いで，培地との接触面から発根が起こる。再
分化は，オーキシン濃度を低下させること，および光を
照射することにより著しく促進される。４週間にわたり
２〜３代以上にわたり継代培養を行なうと，茎葉および
根を有する幼苗が得られる。If the culture is continued, leaves and stems similar to those of the mother plant will grow from the tissue, and then roots will develop from the contact surface with the medium. Regeneration is significantly promoted by lowering auxin concentrations and by irradiation with light. When subculturing is carried out over 2 to 3 generations over a period of 4 weeks, seedlings with stems, leaves, and roots are obtained.
（作用）
本発明方法では，植物ホルモンを含有する培地でＰｉｎ
ｅｌ ｌｉａｅ属植物またはＡｎｇｅｌｉｃａ植物のカ
ルスが有利に誘導される。これらの植物の生長は比較的
遅いにもかかわらず，このカルスの形態においては速や
かに増殖しうる。増殖したカルスを再分化させると出芽
・発根し幼苗が得られる。このように短期間で大量のＰ
ｉｎｅｌｌｉａｅ属またはＡｎｇｅｌｉｃａ属植物の均
質な幼苗が得られる。カルス増殖時に増殖の速いカルス
を選抜すれば，生長の速い植物の幼苗が得られる。例え
ば， Ｐｉｎｅｌｌｉａｅ属植物のカラスビシャクにお
いては，自然の状態では１個の球茎より苗が１本生じ，
秋には３〜６個の球茎が得られるだけであるが，本法に
よれば６ケ月で，５０〜１００本以上の幼苗を形成する
ことが可能となる。(Effect) In the method of the present invention, Pin is grown in a medium containing plant hormones.
Calli of plants of the genus El liae or Angelica plants are advantageously induced. Although the growth of these plants is relatively slow, they can multiply rapidly in this callus form. When the proliferated callus is redifferentiated, it will sprout and root, producing young seedlings. In this way, a large amount of P in a short period of time
Homogeneous seedlings of plants of the genus Inelliae or Angelica are obtained. If callus that multiplies rapidly during callus multiplication is selected, young plant seedlings that grow quickly can be obtained. For example, in the Pinelliae plant, one seedling grows from one corm in its natural state.
Only 3 to 6 corms are obtained in autumn, but according to this method, it is possible to form 50 to 100 or more seedlings in 6 months.
（実施例） 以下に本発明を実施例について述べる。(Example) The present invention will be described below with reference to examples.
実施例１
（Ａ）カルスの誘導：
カラスビシャク（Ｐｉｎｅｌｌｉａｅ ｔｅｒｎａｔ
ａ Ｂｒｅｉｔ）の球茎を適当な大きさに切りとり，
これを７０％エタノール水溶液に３０秒間浸漬し，さら
に次亜塩素酸ナ｝ ＩＪウム水溶液（ＣＩ濃度１．５％
）に８分間浸漬して殺菌処理を行った。これを無菌水で
洗浄後０．５〜２．Ｏｃｔの大きさに切断し，カルス誘
導用培地に置床した。カルス誘導用培地としてはオーキ
シン類として２・４Ｄを１ ｐｐｍの割合で，サイトカ
イニン類としてカイネチンを０．１ｐｐｍの割合で含有
するムラシゲースクーグの寒天培地を用いた。Example 1 (A) Induction of callus: Pinelliae ternat
Cut the corm of a Breit into an appropriate size,
This was immersed in a 70% ethanol aqueous solution for 30 seconds, and further soaked in a sodium hypochlorite aqueous solution (CI concentration 1.5%).
) for 8 minutes for sterilization. After washing this with sterile water, 0.5~2. It was cut to a size of Oct. and placed on a callus induction medium. As a callus induction medium, a Murashige-Skoog agar medium containing 2.4D as an auxin at a rate of 1 ppm and kinetin as a cytokinin at a rate of 0.1 ppm was used.
２５℃で暗所にて２０日間培養を行ったところ切口にカ
ルスが形成された。When cultured in the dark at 25°C for 20 days, callus was formed at the cut end.
（Ｂ）カルスの増殖： （Ａ）項で形成されたカルスを
カルス増殖用培地に移植し．２５℃で２週間培養を行っ
た。カルス増殖用培地としては，オーキシン類としてＮ
ＡＡを２ｐｐｍの割合で，そしてサイトカイニン類とし
てペンジルアデニンを０．５ｐｐｍの割合で含有するム
ラシゲースクーグの寒天培地を用いた。(B) Proliferation of callus: The callus formed in section (A) was transplanted to a callus growth medium. Culture was performed at 25°C for 2 weeks. As a medium for callus growth, N is used as an auxin.
Murashige Skoog's agar medium containing AA at a rate of 2 ppm and penzyladenine as a cytokinin at a rate of 0.5 ppm was used.
（Ｃ）カルスの再分化：サイトカイニン類としてカイネ
チンを１，Ｏｐｐｍの割合で含有し，オーキシン類を含
有しないムラシゲースクーグの寒天培地を調製し再分化
用培地とした。（Ｂ）項で得られた増殖カルスをこの再
分化用培地に置床し， ２０００ルクスの光をあてて２
５℃にて培養を行った。置床後約３０日間で出芽・発根
がはじまり約４ケ月後には２〜４ ｃｍに生長した多数
の幼苗が得られた。このように，カラスビシャクの球茎
から発芽させる方法や挿し木による増殖法に比べ極めて
短時間で幼苗が得られる。(C) Regeneration of callus: A Murashige-Skoog agar medium containing kinetin as a cytokinin at a ratio of 1.0 ppm and no auxins was prepared and used as a regeneration medium. The proliferated callus obtained in section (B) was placed on this regeneration medium and exposed to 2000 lux light for 2 hours.
Culture was performed at 5°C. Approximately 30 days after placing the seedlings, germination and rooting began, and approximately 4 months later, many seedlings that had grown to 2 to 4 cm were obtained. In this way, seedlings can be obtained in an extremely short time compared to the method of germinating from the corm of crowfish or propagating by cuttings.
実施例２
（Ａ）カルスの誘導：実施例１ （Ａ）項と同様である
。Example 2 (A) Induction of callus: Same as Example 1 (A).
（Ｂ）カルスの増殖：才一キシン類としてＮＡＡを１
ｐｐｍの割合で，そしてサイトカイニン類としてカイネ
チンをｌｏｐｐｍの割合で含有するムラシゲースクーグ
の液体培地をカルス増殖用培地としたこと以外は実施例
１ （Ｂ）項と同様である。(B) Proliferation of callus: NAA is used as a stimulant.
The procedure was the same as in Example 1 (B) except that the callus growth medium was a Murashige-Skoog liquid medium containing kinetin as a cytokinin at a loppm ratio.
（Ｃ）本実施例（Ｂ）項で得られた増殖カルスを用い，
実施例１ （Ｃ）項に準じてカルスを再分化させた。置
床後約３０日間で出芽・発根がはじまり約２ケ月後には
２〜４ｃｍに成長した多数の幼苗が得られた。(C) Using the proliferated callus obtained in section (B) of this example,
Example 1 Callus was redifferentiated according to Section (C). Approximately 30 days after placement, germination and rooting began, and approximately 2 months later, many seedlings that had grown to 2 to 4 cm were obtained.
実施例３
カラスビシャクの球茎の代わりに．二ホントウキ（Ｌｉ
ｇｕｓｔｉｃｕｍ ａｃｕｔｉｌｏｂｕｍ Ｓｉｅ
ｂ Ｚｕｃｃ．）の伸長する芽の先端を用い，実施例１
と同様にカルスを誘導・増殖し，さらに再分化を行なっ
た。実施例１とほぼ同様の経過をたどり，多数の二ホン
トウキの幼苗が得られた。Example 3 In place of the corms of the crow's shank. Nihon Touki (Li
gusticum acutilobum Sie
b Zucc. Example 1
Callus was induced and proliferated in the same manner as described above, and further redifferentiation was performed. The process was almost the same as in Example 1, and a large number of young seedlings of Nihontouki were obtained.
実施例４
カラスビシャクの球茎の代わりに．二ホントウキ（Ｌｉ
ｇｕｓｔｉｃｕｍ ａｃｕｔｉｌｏｂｕｍ Ｓｉｅ
ｂ Ｚｕｃｃ．）の伸長する芽の先端を用い，実施例２
と同様にカルスを誘導・増殖し，さらに再分化を行なっ
た。実施例２とほぼ同様の経過をたどり，多数の二ホン
トウキの幼苗が得られた。Example 4 In place of the corm of the crow's-eye. Nihon Touki (Li
gusticum acutilobum Sie
b Zucc. Example 2
Callus was induced and proliferated in the same manner as described above, and further redifferentiation was performed. The process was almost the same as in Example 2, and a large number of young seedlings of Nihontouki were obtained.
（発明の効果）
本発明によれば．このようにＰｉｎｅｌｌｉａｅ属植物
またはＡｎｇｅｌｉｃａ属植物の幼苗が気候，土壌，季
節などに左右されることなく短期間のうちに効果的に得
られる。カルスを適宜選択することにより成長が速く，
かつ有効成分を多量に含む植物体を形成し得る苗が得ら
れる。本法により，カラスビシャク，トウキなどの漢方
薬として有用な植物が安価に生産され得る。(Effect of the invention) According to the present invention. In this way, young seedlings of plants of the genus Pinelliae or plants of the genus Angelica can be effectively obtained in a short period of time without being affected by climate, soil, season, etc. Proper selection of callus will result in faster growth.
Moreover, seedlings capable of forming plants containing a large amount of the active ingredient can be obtained. By this method, plants useful as Chinese herbal medicines, such as Japanese herbal cucumber and Japanese apricot, can be produced at low cost.
以上that's all
Claims (1)
物の組織をオーキシン類およびサイトカイニン類を含有
するカルス誘導用培地を用いて培養しカルスを誘導する
工程、 該カルスをカルス増殖用培地で増殖させる工程、および 該カルスを再分化用培地で光を照射して分化させ幼苗と
する工程、 を包含するＰｉｎｅｌｌｉａｅ属またはＡｎｇｅｌｉｃ
ａ属植物の育苗方法。 ２、前記再分化用培地のオーキシン類の濃度が前記カル
ス増殖用培地のオーキシン類の濃度以下である特許請求
の範囲第１項に記載の方法。 ３、前記照射光の照度が１０００〜１００００ルクスで
ある特許請求の範囲第１項に記載の方法。[Claims] 1. A step of inducing callus by culturing tissue of a plant of the genus Pinelliae or Angelica using a callus induction medium containing auxins and cytokinins, and growing the callus in a callus growth medium. and a step of irradiating the callus with light in a redifferentiation medium to differentiate it into young seedlings.
A method for raising seedlings of plants of the genus A. 2. The method according to claim 1, wherein the concentration of auxins in the regeneration medium is lower than the concentration of auxins in the callus growth medium. 3. The method according to claim 1, wherein the illuminance of the irradiation light is 1,000 to 10,000 lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1053215A JPH02231023A (en) | 1989-03-06 | 1989-03-06 | Raising seedling of genus pinelliae or genus angelic plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1053215A JPH02231023A (en) | 1989-03-06 | 1989-03-06 | Raising seedling of genus pinelliae or genus angelic plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02231023A true JPH02231023A (en) | 1990-09-13 |
Family
ID=12936609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1053215A Pending JPH02231023A (en) | 1989-03-06 | 1989-03-06 | Raising seedling of genus pinelliae or genus angelic plant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02231023A (en) |
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| CN102919122A (en) * | 2012-10-19 | 2013-02-13 | 遵义市龙驰生物科技有限公司 | High-efficiency method for inducing pinellia in vitro bulb |
| CN104396485A (en) * | 2014-10-24 | 2015-03-11 | 定西市农业科学研究院 | Sunlight greenhouse seedling-raising method for improving Chinese angelica germination rate |
| CN104686335A (en) * | 2015-02-22 | 2015-06-10 | 刘木娇 | Tissue culture and rapid propagation method for pinellia ternate |
| CN104705066A (en) * | 2015-04-14 | 2015-06-17 | 四川新绿色药业科技发展股份有限公司 | Ligusticum wallichii cultivation method capable of reducing plant pest and disease damage and increasing survival rate and yield |
| CN104737763A (en) * | 2015-04-08 | 2015-07-01 | 江苏碧云天农林科技有限公司 | Cutting propagation method for angelica keiskei |
| CN104871765A (en) * | 2015-05-04 | 2015-09-02 | 华容县保丰中药材种植专业合作社 | Angelica dahurica planting technique |
| CN105230285A (en) * | 2015-10-16 | 2016-01-13 | 金寨县绿野中药材专业合作社 | Method for planting pinellia cordata |
| CN105409546A (en) * | 2015-12-02 | 2016-03-23 | 云南希尔康种植有限公司 | Method for interplanting radix angelicae in ginkgo biloba forest |
| CN105493853A (en) * | 2015-12-19 | 2016-04-20 | 界首市众鑫科技服务有限公司 | Method for planting radix angelicae dahuricae |
| CN105900839A (en) * | 2016-04-25 | 2016-08-31 | 沈阳药科大学 | Ligusticum jeholense nakai et kitag callus culture method |
| CN109699496A (en) * | 2019-02-25 | 2019-05-03 | 汉江师范学院 | A kind of chaste tree pinellia tuber excised tuber rapid propagation method |
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-
1989
- 1989-03-06 JP JP1053215A patent/JPH02231023A/en active Pending
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|---|---|---|---|---|
| CN102919122A (en) * | 2012-10-19 | 2013-02-13 | 遵义市龙驰生物科技有限公司 | High-efficiency method for inducing pinellia in vitro bulb |
| CN104396485A (en) * | 2014-10-24 | 2015-03-11 | 定西市农业科学研究院 | Sunlight greenhouse seedling-raising method for improving Chinese angelica germination rate |
| CN104686335A (en) * | 2015-02-22 | 2015-06-10 | 刘木娇 | Tissue culture and rapid propagation method for pinellia ternate |
| CN104737763A (en) * | 2015-04-08 | 2015-07-01 | 江苏碧云天农林科技有限公司 | Cutting propagation method for angelica keiskei |
| CN104705066A (en) * | 2015-04-14 | 2015-06-17 | 四川新绿色药业科技发展股份有限公司 | Ligusticum wallichii cultivation method capable of reducing plant pest and disease damage and increasing survival rate and yield |
| CN104871765A (en) * | 2015-05-04 | 2015-09-02 | 华容县保丰中药材种植专业合作社 | Angelica dahurica planting technique |
| CN105230285A (en) * | 2015-10-16 | 2016-01-13 | 金寨县绿野中药材专业合作社 | Method for planting pinellia cordata |
| CN105409546A (en) * | 2015-12-02 | 2016-03-23 | 云南希尔康种植有限公司 | Method for interplanting radix angelicae in ginkgo biloba forest |
| CN105493853A (en) * | 2015-12-19 | 2016-04-20 | 界首市众鑫科技服务有限公司 | Method for planting radix angelicae dahuricae |
| CN105900839A (en) * | 2016-04-25 | 2016-08-31 | 沈阳药科大学 | Ligusticum jeholense nakai et kitag callus culture method |
| CN109757373A (en) * | 2019-01-11 | 2019-05-17 | 长江大学 | A kind of Jing Banxia quick breeding method for tissue culture |
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