JPH0215552Y2 - - Google Patents
Info
- Publication number
- JPH0215552Y2 JPH0215552Y2 JP8883685U JP8883685U JPH0215552Y2 JP H0215552 Y2 JPH0215552 Y2 JP H0215552Y2 JP 8883685 U JP8883685 U JP 8883685U JP 8883685 U JP8883685 U JP 8883685U JP H0215552 Y2 JPH0215552 Y2 JP H0215552Y2
- Authority
- JP
- Japan
- Prior art keywords
- temperature sensor
- container
- holder
- thin diaphragm
- tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 claims description 7
- 239000003566 sealing material Substances 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 230000007797 corrosion Effects 0.000 claims description 2
- 238000005260 corrosion Methods 0.000 claims description 2
- 239000013013 elastic material Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 239000007788 liquid Substances 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000010409 thin film Substances 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Description
【考案の詳細な説明】
〔産業上の利用分野〕
本考案は、主として動植物細胞、その他の微生
物の培養に際して用いられるフラスコ等の容器内
の温度を、容器外から検出し、測定する温度検出
器付き容器に関する。[Detailed description of the invention] [Industrial application field] The present invention is a temperature detector that detects and measures the temperature inside a container such as a flask, which is mainly used for culturing animal and plant cells and other microorganisms, from outside the container. Containers with attached containers.
一般に、動植物の細胞やその他の微生物の培養
に際して用いられるフラスコ等の容器内の温度を
検出するには、容器の蓋体から容器内に吊設され
た温度センサーを培養液中に浸漬し、培養液の温
度を直接検出する方法が行われている。
Generally, to detect the temperature inside a container such as a flask used for culturing animal or plant cells or other microorganisms, a temperature sensor suspended from the lid of the container inside the container is immersed in the culture solution. A method of directly detecting the temperature of the liquid is being used.
しかしながら、この方法によるときは、直接検
出のため、検出温度が培養液温度と一致する確度
が高いが、動植物細胞やその他の微生物の培養に
際して多く行われる振盪培養や撹拌培養には好ま
しい方法とはいえない。 However, when using this method, there is a high probability that the detected temperature will match the temperature of the culture medium due to direct detection, but it is not a preferable method for shaking culture or stirring culture, which is often used when culturing animal, plant, or other microorganisms. I can't say that.
すなわち、振盪培養や撹拌培養においては、容
器内の培養液が均一に振盪され、撹拌されること
は勿論、容器内に設置される撹拌羽根やセンサー
その他の部材に振盪、撹拌による液流が衝突して
細胞が破損されないためにも、液中に障害物がな
るべく少ない方が好ましい。 In other words, in shaking culture or stirring culture, the culture solution in the container is not only shaken and stirred evenly, but also the liquid flow due to shaking and stirring collides with stirring blades, sensors, and other components installed in the container. It is preferable that there be as few obstacles as possible in the liquid so that the cells are not damaged.
したがつて、従来法のように、温度センサーを
培養液中に浸漬して温度を直接検出する方法は、
それだけ培養のための環境条件を悪くする問題点
があつた。 Therefore, the conventional method of directly detecting the temperature by immersing the temperature sensor in the culture solution is
There was a problem that the environmental conditions for culturing were worsened accordingly.
本考案は、このような従来の問題点を解決し、
外部から容器内の液温を正確に検出し、しかも容
器内を閉塞した状態のまま温度センサーの取外し
が可能な温度検出器付き容器を提供しようとする
もので、筒状のホルダーと、該ホルダーの先端開
口面を閉塞する薄隔膜と、ホルダーの後端開口面
から全体を介して挿入され、先端がデイスク状に
形成された温度センサーとからなり、かつ温度セ
ンサー先端面が薄隔膜に圧接された温度検出器
が、側面に検出器取付口が形成された容器の、該
検出器取付口に、Ｏリング状のシール材を介して
挿脱自在に設けられたことを特徴とする。
This invention solves these conventional problems and
The purpose of the present invention is to provide a container equipped with a temperature sensor that can accurately detect the temperature of the liquid inside the container from the outside, and also allows the temperature sensor to be removed while the inside of the container is closed. a thin diaphragm that closes the opening at the tip of the holder, and a temperature sensor that is inserted through the entire rear opening of the holder and has a disk-shaped tip, and the tip of the temperature sensor is pressed against the thin diaphragm. The present invention is characterized in that the temperature sensor is removably inserted into the detector mounting opening of a container having the detector mounting opening formed on the side surface via an O-ring-shaped sealing material.
以下、図に基づいて本考案に係る温度検出器付
き容器の実施例を説明する。
DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of a container with a temperature sensor according to the present invention will be described based on the drawings.
容器としてのフラスコ１の下側には、円筒状の
検出器取付口２が一体に形成される。 A cylindrical detector attachment port 2 is integrally formed on the lower side of the flask 1 as a container.
この検出器取付口２に挿脱可能な大きさからな
るステンレス製で円筒状のホルダー３の後側に
は、フラスコ１の検出器取付口２の端部と衝合
し、ホルダー３の挿入長さを規制するストツパー
を兼ねたフランジ３ａが一体に形成され、このフ
ランジ３ａの後方のホルダー３外周には、螺条部
３ｂが形成されると共に、ホルダー３の先端部
と、略中央部の周囲には、それぞれＯリング嵌入
溝３ｃ，３ｄが鑿設されている。 The rear side of a cylindrical holder 3 made of stainless steel is large enough to be inserted into and removed from the detector mounting port 2, and the end of the detector mounting port 2 of the flask 1 abuts against the end of the holder 3. A flange 3a that also serves as a stopper for regulating the holder 3 is integrally formed, and a threaded portion 3b is formed on the outer periphery of the holder 3 behind the flange 3a, and a threaded portion 3b is formed around the tip and approximately the center of the holder 3. O-ring fitting grooves 3c and 3d are respectively provided in the holes.
各Ｏリング嵌入溝３ｃ，３ｄには、それぞれテ
トラフルオルエチレン製のＯリング４，５が嵌入
され、検出器取付口２の内面と、ホルダー３とを
圧接し、フラスコ１の内外を気密に維持し、かつ
検出器取付口２に対してホルダー３が挿脱自在に
構成される。 O-rings 4 and 5 made of tetrafluoroethylene are fitted into the O-ring fitting grooves 3c and 3d, respectively, and press the inner surface of the detector mounting port 2 and the holder 3, making the inside and outside of the flask 1 airtight. The holder 3 is configured to be able to be inserted into and removed from the detector mounting port 2.
ホルダー３の先端開口面は、１ミル程度の薄膜
からなるテトラフルオルエチレン製の薄隔膜６に
より覆われ、該薄隔膜６の周囲は前記Ｏリング嵌
入溝３ｃに、Ｏリング４により固定される。 The open end surface of the holder 3 is covered with a thin diaphragm 6 made of tetrafluoroethylene made of a thin film of about 1 mil, and the periphery of the thin diaphragm 6 is fixed in the O-ring fitting groove 3c by the O-ring 4. .
ホルダー３の後端開口面からは、先端がデイス
ク状に形成され、そのロツド部７ａがテトラフル
オルエチレン製の栓体８の中心部に摺動自在に圧
入された温度センサー７が、挿入され、温度セン
サー７の先端面が薄隔膜６と圧接されるように、
栓体８がホルダー３の後端開口部に嵌入される。 A temperature sensor 7, which has a disk-shaped tip and whose rod portion 7a is slidably press-fitted into the center of a stopper 8 made of tetrafluoroethylene, is inserted from the rear opening surface of the holder 3. , so that the tip surface of the temperature sensor 7 is brought into pressure contact with the thin diaphragm 6.
The plug body 8 is fitted into the rear end opening of the holder 3.
ホルダー３の螺条部３ｂには、中心部に温度セ
ンサー７のロツド７ａが貫通される貫通孔９ａを
有する袋ナツト９が螺合され、その内面で栓体８
の端面を押圧して、栓体８に対する温度センサー
７のロツド部７ａの摺動抵抗を増大させ、その摺
動が阻止されるようにしたものが用いられる。 A cap nut 9 having a through hole 9a in the center through which the rod 7a of the temperature sensor 7 is passed is screwed into the threaded portion 3b of the holder 3, and the plug 8 is inserted into the cap nut 9 on its inner surface.
The end face of the temperature sensor 7 is pressed to increase the sliding resistance of the rod portion 7a of the temperature sensor 7 against the plug body 8, thereby preventing the rod portion 7a from sliding.
第２図示のように組立てられたフラスコ１の検
出器取付口２から、温度センサー７が取りつけら
れたホルダー３を取り外すには、片方の手でフラ
スコ１を押え、他方の手でホルダー３のフランジ
３ａを摘んでＯリング４，５の圧接力に抗して側
方に引出せば取り外すことができ、逆に上記ホル
ダー３をフラスコ１の検出器取付口２に取りつけ
るには、前記とは逆にホルダー３の前方部を検出
器取付口２内に圧入すればよい。
To remove the holder 3 on which the temperature sensor 7 is attached from the detector attachment port 2 of the flask 1 assembled as shown in the second figure, hold the flask 1 with one hand and press the flange of the holder 3 with the other hand. It can be removed by pinching the holder 3a and pulling it out to the side against the pressure of the O-rings 4 and 5.On the other hand, to attach the holder 3 to the detector mounting port 2 of the flask 1, do the opposite of the above. The front part of the holder 3 may be press-fitted into the detector mounting port 2.
また、温度検出の必要がないか、あるいは培養
が完了して、図示しない振盪機等からフラスコ１
を取り外すには、温度センサー７のみを取り外せ
ばよいから、このときは、袋ナツト９をホルダー
３から外し、栓体８をホルダー３から取り外せ
ば、培養液がフラスコ１から洩れることなく、温
度センサー７のみをフラスコ１から取り外すこと
ができる。 In addition, if there is no need to detect the temperature, or if the culture is completed and the flask is removed from a shaker (not shown), etc.
To remove the temperature sensor 7, only the temperature sensor 7 needs to be removed. At this time, by removing the cap nut 9 from the holder 3 and the stopper 8 from the holder 3, the temperature sensor can be removed without the culture solution leaking from the flask 1. 7 can be removed from flask 1.
上記したように、本考案における温度センサー
の先端がデイスク状であることと、薄隔膜が薄膜
であること、ならびに温度センサーを薄隔膜に圧
接して設けられていることにより、温度センサー
と薄隔膜との密着性が高く、両者間の気相部分は
ほとんど無視でき、容器内の液温を直接液内で検
出すると同様に正確な温度検出が容器外からでき
る。したがつて、容器内に培養の支障となる障害
物を少なくすることができて、振盪培養や撹拌培
養に際しての細胞の破損を可及的に少なくするこ
とができる。
As mentioned above, the tip of the temperature sensor in the present invention is disk-shaped, the thin diaphragm is a thin film, and the temperature sensor is placed in pressure contact with the thin diaphragm. Because of the high adhesion between the two, the gas phase between them can be almost ignored, and the temperature of the liquid in the container can be detected as accurately from outside the container as by directly detecting the temperature inside the liquid. Therefore, it is possible to reduce the number of obstacles that may hinder culture in the container, and to minimize damage to cells during shaking culture or agitation culture.
また、容器と温度検出器、容器と温度センサー
との着脱がきわめて容易に行うことができ、取扱
上も至便である。 Further, the container and the temperature sensor and the container and the temperature sensor can be attached and detached very easily, and handling is convenient.
さらに、筒状のホルダーが、その外周に挿入長
さ規制用のフランジを有するものにおいては、検
出器の容器内への挿入長さが常に一定であるか
ら、容器内で細胞培養を行う場合など、その培養
に支障を生じない適正な位置に温度検出器を細心
の注意を払わなくても設置できると共に、検体
が、テトラフルオルエチレンその他の軟性樹脂も
しくはゴム等の弾性材からなるものにおいては、
温度センサーと薄隔膜との接触を適宜定めた位置
まで摺動させた後、その弾性で温度センサーを設
定位置に固定できて、薄隔膜と温度センサーとの
気相排除を簡単に行うことができ、薄隔膜および
シール材がテトラフルオルエチレン等の耐蝕性材
からなるものにおいては、容器内の培養物の性状
如何にかかわらず、安定した培養が行われ、かつ
温度検出器の耐久性に役立つ等、温度検出器付き
容器としてすぐれた諸効果を有する。 Furthermore, if the cylindrical holder has a flange on its outer periphery to regulate the insertion length, the insertion length of the detector into the container is always constant, so when culturing cells in the container, etc. The temperature detector can be placed in an appropriate position that does not interfere with the culture without the need for extreme caution, and if the specimen is made of tetrafluoroethylene, other soft resins, or elastic materials such as rubber, ,
After sliding the contact between the temperature sensor and the thin diaphragm to an appropriately determined position, its elasticity allows the temperature sensor to be fixed at the set position, making it easy to eliminate the gas phase between the thin diaphragm and the temperature sensor. If the thin diaphragm and sealing material are made of a corrosion-resistant material such as tetrafluoroethylene, stable cultivation will occur regardless of the nature of the culture in the container, and this will contribute to the durability of the temperature sensor. It has various excellent effects as a container with a temperature sensor.
図示するものは、本考案に係る温度検出器付き
容器の実施例を示すもので、第１図は全体の正面
図、第２図は要部の拡大断面図である。
１……フラスコ、２……検出器取付口、３……
ホルダー、３ａ……フランジ、４，５……Ｏリン
グ、６……薄隔膜、７……温度センサー、８……
検体。
What is shown in the drawings shows an embodiment of a container with a temperature sensor according to the present invention, and FIG. 1 is an overall front view, and FIG. 2 is an enlarged sectional view of the main part. 1...Flask, 2...Detector installation port, 3...
Holder, 3a...Flange, 4, 5...O ring, 6...Thin diaphragm, 7...Temperature sensor, 8...
Specimen.
Claims (1)
を閉塞する薄隔膜と、ホルダーの後端開口面か
ら栓体を介して挿入され、先端がデイスク状に
形成された温度センサーとからなり、かつ温度
センサー先端面が薄隔膜に圧接された温度検出
器が、側面に検出器取付口が形成された容器
の、該検出器取付口に、Ｏリング等のシール材
を介して挿脱自在に設けられたことを特徴とす
る温度検出器付き容器。 (2) 筒状のホルダーが、その外周に挿入長さ規制
用のフランジを有するホルダーである実用新案
登録請求の範囲(1)記載の温度検出器付き容器。 (3) 栓体が、テトラフルオルエチレンその他の軟
性樹脂もしくはゴム等の弾性材からなる実用新
案登録請求の範囲(1)および(2)いずれか記載の温
度検出器付き容器。 (4) 薄隔膜およびシール材がテトラフルオルエチ
レン等の耐蝕性材からなる実用新案登録請求の
範囲(1)乃至(3)いずれか記載の温度検出器付き容
器。[Claims for Utility Model Registration] (1) A cylindrical holder, a thin diaphragm that closes the opening at the tip of the holder, and a plug inserted from the opening at the rear end of the holder, with the tip shaped into a disc. A temperature sensor having a temperature sensor formed therein and having a tip end surface pressed against a thin diaphragm is attached to a container having a sensor mounting port formed on the side surface, and an O-ring or the like is attached to the sensor mounting port. A container with a temperature sensor, characterized in that it is installed in a removable manner via a sealing material. (2) The container with a temperature sensor according to claim (1), wherein the cylindrical holder has a flange on its outer periphery for regulating insertion length. (3) A container with a temperature sensor according to any one of claims (1) and (2), in which the stopper is made of tetrafluoroethylene or other soft resin or an elastic material such as rubber. (4) The container with a temperature sensor according to any one of claims (1) to (3), wherein the thin diaphragm and the sealing material are made of a corrosion-resistant material such as tetrafluoroethylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8883685U JPH0215552Y2 (en) | 1985-06-14 | 1985-06-14 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8883685U JPH0215552Y2 (en) | 1985-06-14 | 1985-06-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61204641U JPS61204641U (en) | 1986-12-23 |
| JPH0215552Y2 true JPH0215552Y2 (en) | 1990-04-26 |
Family
ID=30642354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8883685U Expired JPH0215552Y2 (en) | 1985-06-14 | 1985-06-14 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0215552Y2 (en) |
-
1985
- 1985-06-14 JP JP8883685U patent/JPH0215552Y2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61204641U (en) | 1986-12-23 |
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